cationic lipid transfections  (Thermo Fisher)


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    Name:
    Lipofectamine Transfection Reagent
    Description:
    Lipofectamine Transfection Reagent is one of our first generation cationic lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells Lipofectamine Transfection Reagent is a trusted value reagent with many years of cited use in thousands of publications and with many cell lines With Lipofectamine Transfection Reagent you ll get • A reagent proven to work in high throughput applications• A reliable reagent for establishing stable cell lines• A reagent that works well with PLUS Reagent for higher protein expressionA proven technologyOur Lipofectamine brand reagents have been recognized as the most cited transfection reagent family with tens of thousands of citations since launch in 1993 Lipofectamine Transfection Reagent is no exception as a trusted reagent for a wide range of cell lines When used in combination with PLUS Reagent Lipofectamine Transfection Reagent has been shown to work well with cells such as BHK 21 NIH 3T3 COS 1 fibroblasts keratinocytes HT 29 MRC 5 and SK BR3 A value alternative for plasmid DNAWith such a legacy comes a commitment to providing the best reagents for all scientists and labs regardless of their funding capabilities Lipofectamine Transfection Reagent is a value alternative for transfection of plasmid DNA into eukaryotic cells with a simplified protocol For optimal results in a wider range of plasmid DNA transfection conditions we recommend Lipofectamine LTX Reagent due to its optimal balance of potency low cytotoxicity Lipofectamine 2000 Transfection Reagent is recommended if you are transfecting a broad range of cell lines or want to transfect RNA
    Catalog Number:
    18324010
    Price:
    None
    Applications:
    Cell Culture|Plasmid Transfection|Stem Cell & Primary Cell Transfections|Transfection
    Category:
    Cell Culture Transfection Reagents
    Buy from Supplier


    Structured Review

    Thermo Fisher cationic lipid transfections
    SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following <t>transfection</t> with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P
    Lipofectamine Transfection Reagent is one of our first generation cationic lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells Lipofectamine Transfection Reagent is a trusted value reagent with many years of cited use in thousands of publications and with many cell lines With Lipofectamine Transfection Reagent you ll get • A reagent proven to work in high throughput applications• A reliable reagent for establishing stable cell lines• A reagent that works well with PLUS Reagent for higher protein expressionA proven technologyOur Lipofectamine brand reagents have been recognized as the most cited transfection reagent family with tens of thousands of citations since launch in 1993 Lipofectamine Transfection Reagent is no exception as a trusted reagent for a wide range of cell lines When used in combination with PLUS Reagent Lipofectamine Transfection Reagent has been shown to work well with cells such as BHK 21 NIH 3T3 COS 1 fibroblasts keratinocytes HT 29 MRC 5 and SK BR3 A value alternative for plasmid DNAWith such a legacy comes a commitment to providing the best reagents for all scientists and labs regardless of their funding capabilities Lipofectamine Transfection Reagent is a value alternative for transfection of plasmid DNA into eukaryotic cells with a simplified protocol For optimal results in a wider range of plasmid DNA transfection conditions we recommend Lipofectamine LTX Reagent due to its optimal balance of potency low cytotoxicity Lipofectamine 2000 Transfection Reagent is recommended if you are transfecting a broad range of cell lines or want to transfect RNA
    https://www.bioz.com/result/cationic lipid transfections/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    cationic lipid transfections - by Bioz Stars, 2021-01
    99/100 stars

    Images

    1) Product Images from "Knockdown of spindle pole body component 25 homolog inhibits cell proliferation and cycle progression in prostate cancer"

    Article Title: Knockdown of spindle pole body component 25 homolog inhibits cell proliferation and cycle progression in prostate cancer

    Journal: Oncology Letters

    doi: 10.3892/ol.2018.8003

    SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P
    Figure Legend Snippet: SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P

    Techniques Used: Expressing, Transfection, MTT Assay

    2) Product Images from "Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer. Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer"

    Article Title: Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer. Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer

    Journal: The Prostate

    doi: 10.1002/pros.23703

    MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P
    Figure Legend Snippet: MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Techniques Used: Expressing, Polymerase Chain Reaction, Transfection, Western Blot

    3) Product Images from "Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer. Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer"

    Article Title: Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer. Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer

    Journal: The Prostate

    doi: 10.1002/pros.23703

    MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P
    Figure Legend Snippet: MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Techniques Used: Expressing, Polymerase Chain Reaction, Transfection, Western Blot

    4) Product Images from "Knockdown of spindle pole body component 25 homolog inhibits cell proliferation and cycle progression in prostate cancer"

    Article Title: Knockdown of spindle pole body component 25 homolog inhibits cell proliferation and cycle progression in prostate cancer

    Journal: Oncology Letters

    doi: 10.3892/ol.2018.8003

    SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P
    Figure Legend Snippet: SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P

    Techniques Used: Expressing, Transfection, MTT Assay

    Related Articles

    Transfection:

    Article Title: Effect of silencing lncRNATUG1 on rapamycin-induced inhibition of endothelial cell proliferation and migration
    Article Snippet: .. Once HUVECs reached 50–70% confluence, they were seeded in 6-well plates and transfected with 100 nM siTUG1 and siNC using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.), according to the manufacturer's protocol. .. At 24 h post-transfection, DMEM and 100 ng/ml rapamycin (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) were added.

    Article Title: MiR-1180-5p regulates apoptosis of Wilms’ tumor by targeting p73
    Article Snippet: .. Cell transfection was performed using Lipofectamine RNAiMax (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. .. A colorimetric assay with tetrazolium salt was used to assess the antiproliferative effects.

    Article Title: Immunogene therapy with fusogenic nanoparticles modulates macrophage response to Staphylococcus aureus
    Article Snippet: .. Fluorescent dyes Calcein (Sigma-Aldrich) and hydrophobic DiI (Life Technologies) were used and Lipofectamine® 2000 Transfection Reagent was obtained from Thermo Fisher Scientific. .. Custom siRNAs were purchased from Dharmacon (Lafayette, CO) and primers were purchased from IDT DNA (San Diego, CA).

    Article Title: Transformation of human liver L-O2 cells mediated by stable HBx transfection
    Article Snippet: .. NIH/3T3 cells were transfected with Lipofectamine (Invitrogen, USA) as described previously . .. The plasmids pcDNA3 and pCMV-X were used in the transfection experiments.

    Article Title: Interferon-Inducible Cholesterol-25-Hydroxylase Inhibits Hepatitis C Virus Replication via Distinct Mechanisms
    Article Snippet: .. Transfection Plasmid transfections were performed using Lipofectamine 2000 following the manufacturer's instructions (Invitrogen). .. Immunofluorescence staining Cells were fixed in 4% paraformaldehyde, permeabilized with 0.2% Triton X-100/PBS before incubation with primary antibodies (anti-NS3, anti-HA or anti-Flag) for 1 hour at room temperature (RT).

    Article Title: Inhibition of mTORC2/RICTOR Impairs Melanoma Hepatic Metastasis
    Article Snippet: .. Briefly, pre-plated cells were incubated with transfection mixture [Opti-MEM® (Gibco, Waltham, MA, USA), Lipofectamine 2.5% vol/vol, siRNA 50 nM] for 6 hours. .. 48 hours after transfection knock-down efficiency was confirmed by Western blotting and tumor cells were processed for further experiments.

    Article Title: The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer
    Article Snippet: .. Cell transfection was performed with Lipofectamine TM 2000 (Invitrogen) as described in the manufacturer’s protocol. .. Each group of isolated tumour cells was seeded onto 96-well plates in triplicate at a density of 103 /well and incubated for 48 h. Subsequently, the cells were incubated for an additional 2 h in the respective media containing 50 μM EdU (RiboBio, Guangzhou, China).

    Article Title: MicroRNA-17-5p aggravates lipopolysaccharide-induced injury in nasal epithelial cells by targeting Smad7
    Article Snippet: .. Cell transfections were conducted using Lipofectamine 3000 reagent (Invitrogen) as per the manufacturer’s protocol. .. RNAs from the cultured cells were extracted using RNA pure Rapid Extraction Kit (Bioteke Corporation, Beijing, China) according to the manufacturer’s instructions.

    Incubation:

    Article Title: Inhibition of mTORC2/RICTOR Impairs Melanoma Hepatic Metastasis
    Article Snippet: .. Briefly, pre-plated cells were incubated with transfection mixture [Opti-MEM® (Gibco, Waltham, MA, USA), Lipofectamine 2.5% vol/vol, siRNA 50 nM] for 6 hours. .. 48 hours after transfection knock-down efficiency was confirmed by Western blotting and tumor cells were processed for further experiments.

    Plasmid Preparation:

    Article Title: Interferon-Inducible Cholesterol-25-Hydroxylase Inhibits Hepatitis C Virus Replication via Distinct Mechanisms
    Article Snippet: .. Transfection Plasmid transfections were performed using Lipofectamine 2000 following the manufacturer's instructions (Invitrogen). .. Immunofluorescence staining Cells were fixed in 4% paraformaldehyde, permeabilized with 0.2% Triton X-100/PBS before incubation with primary antibodies (anti-NS3, anti-HA or anti-Flag) for 1 hour at room temperature (RT).

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  • 99
    Thermo Fisher cationic lipid transfections
    SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following <t>transfection</t> with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P
    Cationic Lipid Transfections, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cationic lipid transfections/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    cationic lipid transfections - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    Image Search Results


    SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P

    Journal: Oncology Letters

    Article Title: Knockdown of spindle pole body component 25 homolog inhibits cell proliferation and cycle progression in prostate cancer

    doi: 10.3892/ol.2018.8003

    Figure Lengend Snippet: SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P

    Article Snippet: Opti-modified Eagle's medium (Opti-MEM) which was ideal for use during cationic lipid transfections especially Lipofectamine 2000 transfection reagents was purchased from Thermo Fisher Scientific, Inc (cat. no. 31985062).

    Techniques: Expressing, Transfection, MTT Assay

    MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Journal: The Prostate

    Article Title: Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer. Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer

    doi: 10.1002/pros.23703

    Figure Lengend Snippet: MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Article Snippet: Opti‐modified Eagle's medium (Opti‐MEM) which was ideal for use during cationic lipid transfections especially Lipofectamine™ transfection reagents was purchased from Thermo Fisher Scientific, Inc. (Catalog number: 31985062).

    Techniques: Expressing, Polymerase Chain Reaction, Transfection, Western Blot

    MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Journal: The Prostate

    Article Title: Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer. Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer

    doi: 10.1002/pros.23703

    Figure Lengend Snippet: MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Article Snippet: Opti‐modified Eagle's medium (Opti‐MEM) which was ideal for use during cationic lipid transfections especially Lipofectamine™ transfection reagents was purchased from Thermo Fisher Scientific, Inc. (Catalog number: 31985062).

    Techniques: Expressing, Polymerase Chain Reaction, Transfection, Western Blot

    HPV16 evades cGAS/STING responses in HaCaTs during initial infection. cGAS/STING responses to pGL3 and HPV PsV in HaCaTs. (A, B) Cells were either transfected for 90 min with 250 ng pGL3 or infected for the duration of the experiment with 950 ng L1 (equivalent to 250 ng DNA) of HPV16 virions. Cells were harvested at various times post-treatment and cGAS/STING responses assessed via western blotting. (A) IRF3 was phosphorylated, most prominently at 90 min and 4 hr post pGL3 transfection, while IRF3 was not phosphorylated at any time post HPV16 infection, representative blot of n = 4 biological replicates. (B) Densitometric quantification of western blots, n = 4 biological replicates. Statistics calculated by two-way ANOVA ( P interaction = 0.0138) followed by Sidak’s multiple comparison test (*** P

    Journal: PLoS Pathogens

    Article Title: Vesicular trafficking permits evasion of cGAS/STING surveillance during initial human papillomavirus infection

    doi: 10.1371/journal.ppat.1009028

    Figure Lengend Snippet: HPV16 evades cGAS/STING responses in HaCaTs during initial infection. cGAS/STING responses to pGL3 and HPV PsV in HaCaTs. (A, B) Cells were either transfected for 90 min with 250 ng pGL3 or infected for the duration of the experiment with 950 ng L1 (equivalent to 250 ng DNA) of HPV16 virions. Cells were harvested at various times post-treatment and cGAS/STING responses assessed via western blotting. (A) IRF3 was phosphorylated, most prominently at 90 min and 4 hr post pGL3 transfection, while IRF3 was not phosphorylated at any time post HPV16 infection, representative blot of n = 4 biological replicates. (B) Densitometric quantification of western blots, n = 4 biological replicates. Statistics calculated by two-way ANOVA ( P interaction = 0.0138) followed by Sidak’s multiple comparison test (*** P

    Article Snippet: The calculated packaging ratio of 11.16 was therefore used to normalize luciferase data in experiments comparing pGL3 delivery via cationic lipid transfection to wildtype HPV infection (Figs and ).

    Techniques: Infection, Transfection, Western Blot

    HPV-related DNA-responsive cellular genes are not induced by virion infection. Normalized RNA-seq count data were plotted for a subset of 15 DNA-responsive cellular genes selected based on prior literature indicating a relationship with HPV. Error bars represent 95% confidence intervals ( n = 2 for pGL3 transfection, red, and n = 3 for HPV virion infection, black).

    Journal: PLoS Pathogens

    Article Title: Vesicular trafficking permits evasion of cGAS/STING surveillance during initial human papillomavirus infection

    doi: 10.1371/journal.ppat.1009028

    Figure Lengend Snippet: HPV-related DNA-responsive cellular genes are not induced by virion infection. Normalized RNA-seq count data were plotted for a subset of 15 DNA-responsive cellular genes selected based on prior literature indicating a relationship with HPV. Error bars represent 95% confidence intervals ( n = 2 for pGL3 transfection, red, and n = 3 for HPV virion infection, black).

    Article Snippet: The calculated packaging ratio of 11.16 was therefore used to normalize luciferase data in experiments comparing pGL3 delivery via cationic lipid transfection to wildtype HPV infection (Figs and ).

    Techniques: Infection, RNA Sequencing Assay, Transfection

    HPV16 virion infection does not activate cellular responses to dsDNA. RNA-seq was used to transcriptionally profile cellular responses to pGL3 DNA introduced via liposome transfection or HPV virion infection. (A) Relative to H 2 O mock-transfection, there were 142 significantly ( P -adj

    Journal: PLoS Pathogens

    Article Title: Vesicular trafficking permits evasion of cGAS/STING surveillance during initial human papillomavirus infection

    doi: 10.1371/journal.ppat.1009028

    Figure Lengend Snippet: HPV16 virion infection does not activate cellular responses to dsDNA. RNA-seq was used to transcriptionally profile cellular responses to pGL3 DNA introduced via liposome transfection or HPV virion infection. (A) Relative to H 2 O mock-transfection, there were 142 significantly ( P -adj

    Article Snippet: The calculated packaging ratio of 11.16 was therefore used to normalize luciferase data in experiments comparing pGL3 delivery via cationic lipid transfection to wildtype HPV infection (Figs and ).

    Techniques: Infection, RNA Sequencing Assay, Transfection

    HPV16 evades cGAS/STING responses in HFKs during initial infection. cGAS/STING responses to pGL3 and wildtype HPV in primary HFKs. (A) HFKs were either transfected for 90 min with 500 ng pGL3 or infected for the duration of the experiment with 1900 ng L1 (equivalent to 500 ng DNA) of HPV virions. Cells were harvested at various times post-treatment and cGAS/STING responses assessed via western blotting. (A) IRF3 was phosphorylated 4 hr post pGL3 transfection, while IRF3 was not phosphorylated at any time post HPV infection with HPV PsV. representative blot of n = 3 biological replicates. (B) Cells were either transfected for 90 min with 250 ng pGL3 or infected for the duration of the experiment with an equivalent amount of HPV virions. Luciferase activity was measured 24 hr post-treatment, normalized to GAPDH, and corrected for pGL3 encapsidation as described in Materials and Methods . * P

    Journal: PLoS Pathogens

    Article Title: Vesicular trafficking permits evasion of cGAS/STING surveillance during initial human papillomavirus infection

    doi: 10.1371/journal.ppat.1009028

    Figure Lengend Snippet: HPV16 evades cGAS/STING responses in HFKs during initial infection. cGAS/STING responses to pGL3 and wildtype HPV in primary HFKs. (A) HFKs were either transfected for 90 min with 500 ng pGL3 or infected for the duration of the experiment with 1900 ng L1 (equivalent to 500 ng DNA) of HPV virions. Cells were harvested at various times post-treatment and cGAS/STING responses assessed via western blotting. (A) IRF3 was phosphorylated 4 hr post pGL3 transfection, while IRF3 was not phosphorylated at any time post HPV infection with HPV PsV. representative blot of n = 3 biological replicates. (B) Cells were either transfected for 90 min with 250 ng pGL3 or infected for the duration of the experiment with an equivalent amount of HPV virions. Luciferase activity was measured 24 hr post-treatment, normalized to GAPDH, and corrected for pGL3 encapsidation as described in Materials and Methods . * P

    Article Snippet: The calculated packaging ratio of 11.16 was therefore used to normalize luciferase data in experiments comparing pGL3 delivery via cationic lipid transfection to wildtype HPV infection (Figs and ).

    Techniques: Infection, Transfection, Western Blot, Luciferase, Activity Assay