cationic lipid transfections  (Thermo Fisher)


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    • 99
    Name:
    Lipofectamine Transfection Reagent
    Description:
    Lipofectamine Transfection Reagent is one of our first generation cationic lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells Lipofectamine Transfection Reagent is a trusted value reagent with many years of cited use in thousands of publications and with many cell lines With Lipofectamine Transfection Reagent you ll get • A reagent proven to work in high throughput applications• A reliable reagent for establishing stable cell lines• A reagent that works well with PLUS Reagent for higher protein expressionA proven technologyOur Lipofectamine brand reagents have been recognized as the most cited transfection reagent family with tens of thousands of citations since launch in 1993 Lipofectamine Transfection Reagent is no exception as a trusted reagent for a wide range of cell lines When used in combination with PLUS Reagent Lipofectamine Transfection Reagent has been shown to work well with cells such as BHK 21 NIH 3T3 COS 1 fibroblasts keratinocytes HT 29 MRC 5 and SK BR3 A value alternative for plasmid DNAWith such a legacy comes a commitment to providing the best reagents for all scientists and labs regardless of their funding capabilities Lipofectamine Transfection Reagent is a value alternative for transfection of plasmid DNA into eukaryotic cells with a simplified protocol For optimal results in a wider range of plasmid DNA transfection conditions we recommend Lipofectamine LTX Reagent due to its optimal balance of potency low cytotoxicity Lipofectamine 2000 Transfection Reagent is recommended if you are transfecting a broad range of cell lines or want to transfect RNA
    Catalog Number:
    18324010
    Price:
    None
    Applications:
    Cell Culture|Plasmid Transfection|Stem Cell & Primary Cell Transfections|Transfection
    Category:
    Cell Culture Transfection Reagents
    		Buy from Supplier

    Structured Review

    Thermo Fisher cationic lipid transfections
    SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following <t>transfection</t> with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P
    Lipofectamine Transfection Reagent is one of our first generation cationic lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells Lipofectamine Transfection Reagent is a trusted value reagent with many years of cited use in thousands of publications and with many cell lines With Lipofectamine Transfection Reagent you ll get • A reagent proven to work in high throughput applications• A reliable reagent for establishing stable cell lines• A reagent that works well with PLUS Reagent for higher protein expressionA proven technologyOur Lipofectamine brand reagents have been recognized as the most cited transfection reagent family with tens of thousands of citations since launch in 1993 Lipofectamine Transfection Reagent is no exception as a trusted reagent for a wide range of cell lines When used in combination with PLUS Reagent Lipofectamine Transfection Reagent has been shown to work well with cells such as BHK 21 NIH 3T3 COS 1 fibroblasts keratinocytes HT 29 MRC 5 and SK BR3 A value alternative for plasmid DNAWith such a legacy comes a commitment to providing the best reagents for all scientists and labs regardless of their funding capabilities Lipofectamine Transfection Reagent is a value alternative for transfection of plasmid DNA into eukaryotic cells with a simplified protocol For optimal results in a wider range of plasmid DNA transfection conditions we recommend Lipofectamine LTX Reagent due to its optimal balance of potency low cytotoxicity Lipofectamine 2000 Transfection Reagent is recommended if you are transfecting a broad range of cell lines or want to transfect RNA
    https://www.bioz.com/result/cationic lipid transfections/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    cationic lipid transfections - by Bioz Stars, 2020-08
    99/100 stars

    Related Products / Commonly Used Together

    lipofectamine transfection reagents
    opti‐modified eagle 's medium
    opti-modified eagle 's medium
    lipofectamine 2000 transfection reagents

    Images

    1) Product Images from "Knockdown of spindle pole body component 25 homolog inhibits cell proliferation and cycle progression in prostate cancer"

    Article Title: Knockdown of spindle pole body component 25 homolog inhibits cell proliferation and cycle progression in prostate cancer

    Journal: Oncology Letters

    doi: 10.3892/ol.2018.8003

    SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P
    Figure Legend Snippet: SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P

    Techniques Used: Expressing, Transfection, MTT Assay

    2) Product Images from "Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer. Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer"

    Article Title: Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer. Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer

    Journal: The Prostate

    doi: 10.1002/pros.23703

    MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P
    Figure Legend Snippet: MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Techniques Used: Expressing, Polymerase Chain Reaction, Transfection, Western Blot

    3) Product Images from "Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer. Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer"

    Article Title: Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer. Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer

    Journal: The Prostate

    doi: 10.1002/pros.23703

    MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P
    Figure Legend Snippet: MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Techniques Used: Expressing, Polymerase Chain Reaction, Transfection, Western Blot

    4) Product Images from "Knockdown of spindle pole body component 25 homolog inhibits cell proliferation and cycle progression in prostate cancer"

    Article Title: Knockdown of spindle pole body component 25 homolog inhibits cell proliferation and cycle progression in prostate cancer

    Journal: Oncology Letters

    doi: 10.3892/ol.2018.8003

    SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P
    Figure Legend Snippet: SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P

    Techniques Used: Expressing, Transfection, MTT Assay

    Related Articles

    Electroporation:

    Article Title: An Efficient Method for Electroporation of Small Interfering RNAs into ENCODE Project Tier 1 GM12878 and K562 Cell Lines
    Article Snippet: .. K562 cells, a myelogenous leukemia cell line that also belongs to Tier 1 of the ENCODE cell line, are readily transfectable via cationic, lipid-based transfection reagents, , such as Lipofectamine 2000 or RNAiMax (Invitrogen, Life Technologies), as well as electroporation. .. In contrast, numerous lipid-based transfection reagents and conditions tested in GM12878 cells were not successful at different cell densities (5 × 105 , 1 × 106 –4 × 106 GM12878 cells) by use of 150 pmol DDX5 or hnRNP A1 siRNAs (data not shown).

    Transfection:

    Article Title: Human DDX3 Interacts with the HIV-1 Tat Protein to Facilitate Viral mRNA Translation
    Article Snippet: .. Cell transfection was performed using Lipofectamine 2000 (Invitrogen), essentially according to the manufacturer’s instructions. .. To measure HIV-1 production efficiency, HeLa cells were seeded in 6-well culture dishes (2 × 105 cells/well) and transfected with indicated shRNA-expressing vectors (2 µg).

    Article Title: Upregulation of the constitutively expressed HSC70 by KLF4
    Article Snippet: .. Morpholino oligonucleotides were transfected into C2C12 myogenic cells with lipofectamine according to the manufacturer’s instructions (Invitrogen) 24 h after plating. ..

    Article Title: Modulation of miR-10a-mediated TGF-β1/Smads signaling affects atrial fibrillation-induced cardiac fibrosis and cardiac fibroblast proliferation
    Article Snippet: .. Cell transfection was performed with Lipofectamine 2000 (Invitrogen, U.S.A.). .. HE staining and Masson’s trichrome staining Atrial tissues were collected and fixed in Davidson’s solution for 24 h before routine dehydration, hyalinization, wax-dipping, and paraffin embedment.

    Article Title: An Efficient Method for Electroporation of Small Interfering RNAs into ENCODE Project Tier 1 GM12878 and K562 Cell Lines
    Article Snippet: .. K562 cells, a myelogenous leukemia cell line that also belongs to Tier 1 of the ENCODE cell line, are readily transfectable via cationic, lipid-based transfection reagents, , such as Lipofectamine 2000 or RNAiMax (Invitrogen, Life Technologies), as well as electroporation. .. In contrast, numerous lipid-based transfection reagents and conditions tested in GM12878 cells were not successful at different cell densities (5 × 105 , 1 × 106 –4 × 106 GM12878 cells) by use of 150 pmol DDX5 or hnRNP A1 siRNAs (data not shown).

    Article Title: EPB41L3 is a potential tumor suppressor gene and prognostic indicator in esophageal squamous cell carcinoma
    Article Snippet: .. Cell transfection was performed with Lipofectamine 2000 and KYSE-150 and KYSE-510 were selected with 500 µ g/ml G418 sulfate (both from Thermo Fisher Scientific, Inc.) for 2–3 weeks, according to the manufacturer's protocol for stable transfection. .. The KYSE-150 and KYSE-510 cells transfected with the EPB41L3 overexpression plasmid were termed vec-150-epb and vec-510-epb respectively.

    Article Title: MAGI3 negatively regulates Wnt/β-catenin signaling and suppresses malignant phenotypes of glioma cells
    Article Snippet: .. Cell transfection was performed using Lipofectamine 2000 (Invitrogen). .. For siRNA knockdown and transient transfection experiments, RNA or protein extraction was performed 48 h post-transfection.

    Article Title: Antitumor Activity of a Novel Antisense Oligonucleotide Against Akt1
    Article Snippet: .. All media and reagents including Lipofectamine Plus and Lipofectamine 2000 used for transfection and M-MLV enzyme kit were obtained from Invitrogen (Carlsbad, CA). .. RNA-STAT kit was purchased from TEL-TEST, Inc. (Friendswood, TX).

    Cotransfection:

    Article Title: Identification of Critical Amino Acid Residues in Human Immunodeficiency Virus Type 1 IN Required for Efficient Proviral DNA Formation at Steps prior to Integration in Dividing and Nondividing Cells
    Article Snippet: .. Pseudotype viruses were generated by cotransfection of COS cells with the pNL43lucΔenv vector, containing each IN mutation, and an amphotropic Moloney murine leukemia virus (MuLV) envelope expression vector (pJD-1) or a macrophage-tropic HIV envelope expression vector (pJR-FL), using Lipofectamine (GIBCO BRL). .. The culture supernatants (5 ml) of the transfected COS cells were harvested 48 h posttransfection, filtered through 0.45-μm-pore-size filters, and used as virus preparations.

    Stable Transfection:

    Article Title: EPB41L3 is a potential tumor suppressor gene and prognostic indicator in esophageal squamous cell carcinoma
    Article Snippet: .. Cell transfection was performed with Lipofectamine 2000 and KYSE-150 and KYSE-510 were selected with 500 µ g/ml G418 sulfate (both from Thermo Fisher Scientific, Inc.) for 2–3 weeks, according to the manufacturer's protocol for stable transfection. .. The KYSE-150 and KYSE-510 cells transfected with the EPB41L3 overexpression plasmid were termed vec-150-epb and vec-510-epb respectively.

    Mutagenesis:

    Article Title: Identification of Critical Amino Acid Residues in Human Immunodeficiency Virus Type 1 IN Required for Efficient Proviral DNA Formation at Steps prior to Integration in Dividing and Nondividing Cells
    Article Snippet: .. Pseudotype viruses were generated by cotransfection of COS cells with the pNL43lucΔenv vector, containing each IN mutation, and an amphotropic Moloney murine leukemia virus (MuLV) envelope expression vector (pJD-1) or a macrophage-tropic HIV envelope expression vector (pJR-FL), using Lipofectamine (GIBCO BRL). .. The culture supernatants (5 ml) of the transfected COS cells were harvested 48 h posttransfection, filtered through 0.45-μm-pore-size filters, and used as virus preparations.

    Generated:

    Article Title: Identification of Critical Amino Acid Residues in Human Immunodeficiency Virus Type 1 IN Required for Efficient Proviral DNA Formation at Steps prior to Integration in Dividing and Nondividing Cells
    Article Snippet: .. Pseudotype viruses were generated by cotransfection of COS cells with the pNL43lucΔenv vector, containing each IN mutation, and an amphotropic Moloney murine leukemia virus (MuLV) envelope expression vector (pJD-1) or a macrophage-tropic HIV envelope expression vector (pJR-FL), using Lipofectamine (GIBCO BRL). .. The culture supernatants (5 ml) of the transfected COS cells were harvested 48 h posttransfection, filtered through 0.45-μm-pore-size filters, and used as virus preparations.

    Expressing:

    Article Title: Identification of Critical Amino Acid Residues in Human Immunodeficiency Virus Type 1 IN Required for Efficient Proviral DNA Formation at Steps prior to Integration in Dividing and Nondividing Cells
    Article Snippet: .. Pseudotype viruses were generated by cotransfection of COS cells with the pNL43lucΔenv vector, containing each IN mutation, and an amphotropic Moloney murine leukemia virus (MuLV) envelope expression vector (pJD-1) or a macrophage-tropic HIV envelope expression vector (pJR-FL), using Lipofectamine (GIBCO BRL). .. The culture supernatants (5 ml) of the transfected COS cells were harvested 48 h posttransfection, filtered through 0.45-μm-pore-size filters, and used as virus preparations.

    Plasmid Preparation:

    Article Title: Identification of Critical Amino Acid Residues in Human Immunodeficiency Virus Type 1 IN Required for Efficient Proviral DNA Formation at Steps prior to Integration in Dividing and Nondividing Cells
    Article Snippet: .. Pseudotype viruses were generated by cotransfection of COS cells with the pNL43lucΔenv vector, containing each IN mutation, and an amphotropic Moloney murine leukemia virus (MuLV) envelope expression vector (pJD-1) or a macrophage-tropic HIV envelope expression vector (pJR-FL), using Lipofectamine (GIBCO BRL). .. The culture supernatants (5 ml) of the transfected COS cells were harvested 48 h posttransfection, filtered through 0.45-μm-pore-size filters, and used as virus preparations.

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    • cationic lipid transfections  (Thermo Fisher)
    99
    Thermo Fisher cationic lipid transfections
    SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following <t>transfection</t> with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P
    Cationic Lipid Transfections, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cationic lipid transfections/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    cationic lipid transfections - by Bioz Stars, 2020-08
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P

    Journal: Oncology Letters

    Article Title: Knockdown of spindle pole body component 25 homolog inhibits cell proliferation and cycle progression in prostate cancer

    doi: 10.3892/ol.2018.8003

    Figure Lengend Snippet: SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P

    Article Snippet: Opti-modified Eagle's medium (Opti-MEM) which was ideal for use during cationic lipid transfections especially Lipofectamine 2000 transfection reagents was purchased from Thermo Fisher Scientific, Inc (cat. no. 31985062).

    Techniques: Expressing, Transfection, MTT Assay

    MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Journal: The Prostate

    Article Title: Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer. Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer

    doi: 10.1002/pros.23703

    Figure Lengend Snippet: MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Article Snippet: Opti‐modified Eagle's medium (Opti‐MEM) which was ideal for use during cationic lipid transfections especially Lipofectamine™ transfection reagents was purchased from Thermo Fisher Scientific, Inc. (Catalog number: 31985062).

    Techniques: Expressing, Polymerase Chain Reaction, Transfection, Western Blot

    MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Journal: The Prostate

    Article Title: Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer. Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer

    doi: 10.1002/pros.23703

    Figure Lengend Snippet: MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Article Snippet: Opti‐modified Eagle's medium (Opti‐MEM) which was ideal for use during cationic lipid transfections especially Lipofectamine™ transfection reagents was purchased from Thermo Fisher Scientific, Inc. (Catalog number: 31985062).

    Techniques: Expressing, Polymerase Chain Reaction, Transfection, Western Blot

    SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P

    Journal: Oncology Letters

    Article Title: Knockdown of spindle pole body component 25 homolog inhibits cell proliferation and cycle progression in prostate cancer

    doi: 10.3892/ol.2018.8003

    Figure Lengend Snippet: SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P

    Article Snippet: Opti-modified Eagle's medium (Opti-MEM) which was ideal for use during cationic lipid transfections especially Lipofectamine 2000 transfection reagents was purchased from Thermo Fisher Scientific, Inc (cat. no. 31985062).

    Techniques: Expressing, Transfection, MTT Assay