cationic lipid reagent mediated transfection technique  (Thermo Fisher)


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    Name:
    DMRIE C Transfection Reagent
    Description:
    DMRIE C Transfection Reagent is suitable for transfecting DNA and RNA into eukaryotic cells and is particularly effective for transfecting suspension cells e g Jurkat and other lymphoid derived cell lines Refer to the Cell Lines database at www invitrogen com for a list of cell types successfully transfected DMRIE C can also be used for in vivo delivery of DNA DMRIE C is a 1 1 M M liposome formulation of the cationic lipid DMRIE 1 2 dimyristyloxy propyl 3 dimethyl hydroxy ethyl ammonium bromide and cholesterol in membrane filtered water
    Catalog Number:
    10459014
    Price:
    None
    Applications:
    Cell Culture|Plasmid Transfection|Transfection
    Category:
    Cell Culture Transfection Reagents
    Buy from Supplier


    Structured Review

    Thermo Fisher cationic lipid reagent mediated transfection technique
    (a) Overexpression of ADARs by cotransfection of either an ADAR1 (pSS151) or ADAR2 (pSKW005) plasmid with pNL4-3 enhanced HIV-1 p24 production, as shown by p24 ELISA of the culture supernatant of transfected COS-7 cells. The <t>transfection</t> efficiencies,
    DMRIE C Transfection Reagent is suitable for transfecting DNA and RNA into eukaryotic cells and is particularly effective for transfecting suspension cells e g Jurkat and other lymphoid derived cell lines Refer to the Cell Lines database at www invitrogen com for a list of cell types successfully transfected DMRIE C can also be used for in vivo delivery of DNA DMRIE C is a 1 1 M M liposome formulation of the cationic lipid DMRIE 1 2 dimyristyloxy propyl 3 dimethyl hydroxy ethyl ammonium bromide and cholesterol in membrane filtered water
    https://www.bioz.com/result/cationic lipid reagent mediated transfection technique/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cationic lipid reagent mediated transfection technique - by Bioz Stars, 2020-09
    99/100 stars

    Related Products / Commonly Used Together

    pnl4-3
    pss43
    pskw005
    pss151

    Images

    1) Product Images from "Double-Stranded RNA Adenosine Deaminases Enhance Expression of Human Immunodeficiency Virus Type 1 Proteins ▿"

    Article Title: Double-Stranded RNA Adenosine Deaminases Enhance Expression of Human Immunodeficiency Virus Type 1 Proteins ▿

    Journal:

    doi: 10.1128/JVI.00238-08

    (a) Overexpression of ADARs by cotransfection of either an ADAR1 (pSS151) or ADAR2 (pSKW005) plasmid with pNL4-3 enhanced HIV-1 p24 production, as shown by p24 ELISA of the culture supernatant of transfected COS-7 cells. The transfection efficiencies,
    Figure Legend Snippet: (a) Overexpression of ADARs by cotransfection of either an ADAR1 (pSS151) or ADAR2 (pSKW005) plasmid with pNL4-3 enhanced HIV-1 p24 production, as shown by p24 ELISA of the culture supernatant of transfected COS-7 cells. The transfection efficiencies,

    Techniques Used: Over Expression, Cotransfection, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Transfection

    Related Articles

    Transfection:

    Article Title: Cell Culture-Adaptive Mutations Promote Viral Protein-Protein Interactions and Morphogenesis of Infectious Hepatitis C Virus
    Article Snippet: .. To produce infectious HCV, HCV RNAs were transfected into Huh-7.5 cells using DMRIE-C reagent (Invitrogen), following the manufacturer's instructions. .. At 72 h posttransfection (p.t.), Huh-7.5 cells were either lysed for detection of HCV proteins or used for isolation of total RNAs and preparation of intracellular HCV.

    Article Title: Differential Upregulation of p53-Responsive Genes by Genotoxic Stress in Hematopoietic Cells Containing Wild-Type and Mutant p53
    Article Snippet: .. MOLT-4 and U266 cells were transfected using the DMRIE-C reagent, a lipofectine derivative using the manufacturer’s instructions (Life Technologies Inc). ..

    Article Title: A generic RNA-pulsed dendritic cell vaccine strategy for renal cell carcinoma
    Article Snippet: .. Transfection of DCs Transfection of DCs with RNA was carried out using the cationic lipid reagent DMRIE-C (Invitrogen, Karlsruhe, Germany). .. On day 7, iDCs were harvested and resuspended in OptiMEM® I (Invitrogen) at a final concentration of 1.0 × 106 DCs/ml.

    Article Title: Interference of ribosomal frameshifting by antisense peptide nucleic acids suppresses SARS coronavirus replication
    Article Snippet: .. Vero cells grown in a 24-well plate were transfected with 0.5 μg pJD464 or pJD502 plasmids using Fugene 6 (Roche Applied Science) or DMRIE-C (Invitrogen) transfection reagents. .. For transfection of BHK-21 with a SARS-CoV replicon plasmid, cells (2.5 × 106 ) in 400 μl Opti-MEM I reduced-serum medium (Gibco BRL) were electroporated with SARS-CoV replicon plasmids (16 μg) at 260 V and 950 μF in a 4 mm electrode gap cuvette (Bio-Rad Laboratories) using a Gene Pulser II electroporator (Bio-Rad Laboratories).

    Article Title: Targeted transcriptional repression of Gfi1 by GFI1 and GFI1B in lymphoid cells
    Article Snippet: .. Transient transfections of Jurkat cells were performed with DMRIE-C (Invitrogen) according to the manufacturer’s protocol. ..

    Article Title: Anoxia-Induced Up-Regulation of Interleukin-8 in Human Malignant Melanoma
    Article Snippet: .. Melanoma cell lines MV3 and 530 were transfected with 2 μg of appropriate plasmid DNA using the DMRIE-C (1,2-dimyristyloxypropyl-3-dimethyl-hydroxyethyl ammonium bromide) reagent (Gibco, Life Technologies, Eggenstein, Germany) according to the manufacturer’s specifications. ..

    Article Title: Identification of dispensable nucleotide sequence in 3′ untranslated region of porcine reproductive and respiratory syndrome virus
    Article Snippet: .. About 2 μl of the synthetic RNA was transfected into MARC-145 cell monolayer in duplicated 6-well plates with DMRIE-C (Invitrogen). ..

    Plasmid Preparation:

    Article Title: Anoxia-Induced Up-Regulation of Interleukin-8 in Human Malignant Melanoma
    Article Snippet: .. Melanoma cell lines MV3 and 530 were transfected with 2 μg of appropriate plasmid DNA using the DMRIE-C (1,2-dimyristyloxypropyl-3-dimethyl-hydroxyethyl ammonium bromide) reagent (Gibco, Life Technologies, Eggenstein, Germany) according to the manufacturer’s specifications. ..

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    Thermo Fisher cationic lipid transfections
    SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following <t>transfection</t> with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P
    Cationic Lipid Transfections, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cationic lipid transfections/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    cationic lipid transfections - by Bioz Stars, 2020-09
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    85
    Thermo Fisher lipid based transient transfection
    Measurements of PKA activation using the AKAR FRET biosensor for RI α −/− cells expressing the various RI α constructs. (A, B) Examples of RI α −/− cells expressing the indicated constructs stimulated with 8-CPT-cAMP or 8-CPT-cGMP at t = 0 (minutes). Cell images as shown were pseudocolored according to the calculated YFP/CFP FRET ratios, with the range displayed indicated on the color bars at right. Left panels show the tracings of the FRET ratios as % change over time. Scale bars: 10 μ M. (C) Box plots of the data showing all 8-CPT-cAMP and 8-CPT-cGMP cell stimulations plotted as % change of the normalized FRET ratios. Total number of individual cells analyzed for each condition is between 20 and 26, encompassing multiple independently conducted stimulations and <t>transfections.</t>
    Lipid Based Transient Transfection, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transient transfection/product/Thermo Fisher
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lipid based transient transfection - by Bioz Stars, 2020-09
    85/100 stars
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    Image Search Results


    SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P

    Journal: Oncology Letters

    Article Title: Knockdown of spindle pole body component 25 homolog inhibits cell proliferation and cycle progression in prostate cancer

    doi: 10.3892/ol.2018.8003

    Figure Lengend Snippet: SPC25 knockdown inhibits the proliferation of PC-3 cells. (A) Expression of SPC25 mRNA following transfection with the indicated shRNAs in PC-3 cells. (B) An MTT assay demonstrated that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (C) The Celigo ® system revealed that knockdown of SPC25 inhibited cell proliferation in PC-3 cells. (D) Cell number of shCtrl or shSPC25 group in each day was calculated using the Celigo ® system. P

    Article Snippet: Opti-modified Eagle's medium (Opti-MEM) which was ideal for use during cationic lipid transfections especially Lipofectamine 2000 transfection reagents was purchased from Thermo Fisher Scientific, Inc (cat. no. 31985062).

    Techniques: Expressing, Transfection, MTT Assay

    MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Journal: The Prostate

    Article Title: Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer. Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer

    doi: 10.1002/pros.23703

    Figure Lengend Snippet: MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Article Snippet: Opti‐modified Eagle's medium (Opti‐MEM) which was ideal for use during cationic lipid transfections especially Lipofectamine™ transfection reagents was purchased from Thermo Fisher Scientific, Inc. (Catalog number: 31985062).

    Techniques: Expressing, Polymerase Chain Reaction, Transfection, Western Blot

    MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Journal: The Prostate

    Article Title: Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer. Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer

    doi: 10.1002/pros.23703

    Figure Lengend Snippet: MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Article Snippet: Opti‐modified Eagle's medium (Opti‐MEM) which was ideal for use during cationic lipid transfections especially Lipofectamine™ transfection reagents was purchased from Thermo Fisher Scientific, Inc. (Catalog number: 31985062).

    Techniques: Expressing, Polymerase Chain Reaction, Transfection, Western Blot

    Measurements of PKA activation using the AKAR FRET biosensor for RI α −/− cells expressing the various RI α constructs. (A, B) Examples of RI α −/− cells expressing the indicated constructs stimulated with 8-CPT-cAMP or 8-CPT-cGMP at t = 0 (minutes). Cell images as shown were pseudocolored according to the calculated YFP/CFP FRET ratios, with the range displayed indicated on the color bars at right. Left panels show the tracings of the FRET ratios as % change over time. Scale bars: 10 μ M. (C) Box plots of the data showing all 8-CPT-cAMP and 8-CPT-cGMP cell stimulations plotted as % change of the normalized FRET ratios. Total number of individual cells analyzed for each condition is between 20 and 26, encompassing multiple independently conducted stimulations and transfections.

    Journal: ACS chemical biology

    Article Title: Switching Cyclic Nucleotide-Selective Activation of Cyclic Adenosine Monophosphate-Dependent Protein Kinase Holoenzyme Reveals Distinct Roles of Tandem Cyclic Nucleotide-Binding Domains

    doi: 10.1021/acschembio.7b00732

    Figure Lengend Snippet: Measurements of PKA activation using the AKAR FRET biosensor for RI α −/− cells expressing the various RI α constructs. (A, B) Examples of RI α −/− cells expressing the indicated constructs stimulated with 8-CPT-cAMP or 8-CPT-cGMP at t = 0 (minutes). Cell images as shown were pseudocolored according to the calculated YFP/CFP FRET ratios, with the range displayed indicated on the color bars at right. Left panels show the tracings of the FRET ratios as % change over time. Scale bars: 10 μ M. (C) Box plots of the data showing all 8-CPT-cAMP and 8-CPT-cGMP cell stimulations plotted as % change of the normalized FRET ratios. Total number of individual cells analyzed for each condition is between 20 and 26, encompassing multiple independently conducted stimulations and transfections.

    Article Snippet: In additional experiments, lipid-based transient transfection was carried out using Lipofectamine 2000 (Thermo-Fisher).

    Techniques: Activation Assay, Expressing, Construct, Cycling Probe Technology, Transfection