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Promega cationic lipid based transfection reagent
In vitro gene silencing efficiency of various concentrations of siRNA (0.1, 0.2, 0.4 and 0.8 ng/µl) with/without mTat/PEI, INT, and mTat/PEI/INT in HSC-3 cells. In vitro <t>transfection</t> efficiency of siRNA with/without mTat/PEI, INT (0.015, 0.03 and 0.06% v/v), and mTat/PEI/INT (0.015, 0.03, and 0.06% v/v) in HSC-3 cells ( a ). β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( b ). Transfection efficiency of siRNA with mTat/PEI/INT at the different concentration. siRNA concentration was 0.8 ng/µl ( c ). β-actin protein was measured in the cell lysate of HSC-3 cells transfected with siRNA with/without mTat/PEI, INT, and mTat/PEI/INT incubated for 48 h ( d ). siRNA concentration was 0.8 ng/µl and INT concentration was 0.06% v/v. Significant difference between INT/siRNA and mTat/PEI/INT/siRNA, * p
Cationic Lipid Based Transfection Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cationic lipid based transfection reagent/product/Promega
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
cationic lipid based transfection reagent - by Bioz Stars, 2020-08
93/100 stars

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1) Product Images from "Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway"

Article Title: Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway

Journal: Journal of Nanobiotechnology

doi: 10.1186/s12951-019-0444-8

In vitro gene silencing efficiency of various concentrations of siRNA (0.1, 0.2, 0.4 and 0.8 ng/µl) with/without mTat/PEI, INT, and mTat/PEI/INT in HSC-3 cells. In vitro transfection efficiency of siRNA with/without mTat/PEI, INT (0.015, 0.03 and 0.06% v/v), and mTat/PEI/INT (0.015, 0.03, and 0.06% v/v) in HSC-3 cells ( a ). β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( b ). Transfection efficiency of siRNA with mTat/PEI/INT at the different concentration. siRNA concentration was 0.8 ng/µl ( c ). β-actin protein was measured in the cell lysate of HSC-3 cells transfected with siRNA with/without mTat/PEI, INT, and mTat/PEI/INT incubated for 48 h ( d ). siRNA concentration was 0.8 ng/µl and INT concentration was 0.06% v/v. Significant difference between INT/siRNA and mTat/PEI/INT/siRNA, * p
Figure Legend Snippet: In vitro gene silencing efficiency of various concentrations of siRNA (0.1, 0.2, 0.4 and 0.8 ng/µl) with/without mTat/PEI, INT, and mTat/PEI/INT in HSC-3 cells. In vitro transfection efficiency of siRNA with/without mTat/PEI, INT (0.015, 0.03 and 0.06% v/v), and mTat/PEI/INT (0.015, 0.03, and 0.06% v/v) in HSC-3 cells ( a ). β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( b ). Transfection efficiency of siRNA with mTat/PEI/INT at the different concentration. siRNA concentration was 0.8 ng/µl ( c ). β-actin protein was measured in the cell lysate of HSC-3 cells transfected with siRNA with/without mTat/PEI, INT, and mTat/PEI/INT incubated for 48 h ( d ). siRNA concentration was 0.8 ng/µl and INT concentration was 0.06% v/v. Significant difference between INT/siRNA and mTat/PEI/INT/siRNA, * p

Techniques Used: In Vitro, Transfection, Quantitative RT-PCR, Expressing, Concentration Assay, Incubation

Zeta potentials ( a ) and particle sizes (diameter) ( b ) of siRNA, siRNA with Tat/PEI, INT, and siRNA with mTat/PEI/INT. Effects of temperature on siRNA transfection efficiency with mTat/PEI, INT, or mTat/PEI/INT in HSC-3 cells at 4 °C (white bars) or 37 °C (black bars) ( c ). * p
Figure Legend Snippet: Zeta potentials ( a ) and particle sizes (diameter) ( b ) of siRNA, siRNA with Tat/PEI, INT, and siRNA with mTat/PEI/INT. Effects of temperature on siRNA transfection efficiency with mTat/PEI, INT, or mTat/PEI/INT in HSC-3 cells at 4 °C (white bars) or 37 °C (black bars) ( c ). * p

Techniques Used: Transfection

Comparative transfection efficiency of FuGENE HD (FuGENE), X-tremeGENE (X-treme), SuperFect (Super), Lipofectamine 2000 (L-2000), Lipofectamine RNAiMAX (L-iMAX), INT, and mTat/PEI/INT using siRNA targeting β - actin in HSC-3 cells. β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( a ). Cell viability of each of the reagent groups in HaCaT cells were evaluated by MTT assay under the same siRNA and transfection reagent volume ( b ). * p
Figure Legend Snippet: Comparative transfection efficiency of FuGENE HD (FuGENE), X-tremeGENE (X-treme), SuperFect (Super), Lipofectamine 2000 (L-2000), Lipofectamine RNAiMAX (L-iMAX), INT, and mTat/PEI/INT using siRNA targeting β - actin in HSC-3 cells. β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( a ). Cell viability of each of the reagent groups in HaCaT cells were evaluated by MTT assay under the same siRNA and transfection reagent volume ( b ). * p

Techniques Used: Transfection, Quantitative RT-PCR, Expressing, MTT Assay

2) Product Images from "Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway"

Article Title: Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway

Journal: Journal of Nanobiotechnology

doi: 10.1186/s12951-019-0444-8

In vitro gene silencing efficiency of various concentrations of siRNA (0.1, 0.2, 0.4 and 0.8 ng/µl) with/without mTat/PEI, INT, and mTat/PEI/INT in HSC-3 cells. In vitro transfection efficiency of siRNA with/without mTat/PEI, INT (0.015, 0.03 and 0.06% v/v), and mTat/PEI/INT (0.015, 0.03, and 0.06% v/v) in HSC-3 cells ( a ). β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( b ). Transfection efficiency of siRNA with mTat/PEI/INT at the different concentration. siRNA concentration was 0.8 ng/µl ( c ). β-actin protein was measured in the cell lysate of HSC-3 cells transfected with siRNA with/without mTat/PEI, INT, and mTat/PEI/INT incubated for 48 h ( d ). siRNA concentration was 0.8 ng/µl and INT concentration was 0.06% v/v. Significant difference between INT/siRNA and mTat/PEI/INT/siRNA, * p
Figure Legend Snippet: In vitro gene silencing efficiency of various concentrations of siRNA (0.1, 0.2, 0.4 and 0.8 ng/µl) with/without mTat/PEI, INT, and mTat/PEI/INT in HSC-3 cells. In vitro transfection efficiency of siRNA with/without mTat/PEI, INT (0.015, 0.03 and 0.06% v/v), and mTat/PEI/INT (0.015, 0.03, and 0.06% v/v) in HSC-3 cells ( a ). β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( b ). Transfection efficiency of siRNA with mTat/PEI/INT at the different concentration. siRNA concentration was 0.8 ng/µl ( c ). β-actin protein was measured in the cell lysate of HSC-3 cells transfected with siRNA with/without mTat/PEI, INT, and mTat/PEI/INT incubated for 48 h ( d ). siRNA concentration was 0.8 ng/µl and INT concentration was 0.06% v/v. Significant difference between INT/siRNA and mTat/PEI/INT/siRNA, * p

Techniques Used: In Vitro, Transfection, Quantitative RT-PCR, Expressing, Concentration Assay, Incubation

Zeta potentials ( a ) and particle sizes (diameter) ( b ) of siRNA, siRNA with Tat/PEI, INT, and siRNA with mTat/PEI/INT. Effects of temperature on siRNA transfection efficiency with mTat/PEI, INT, or mTat/PEI/INT in HSC-3 cells at 4 °C (white bars) or 37 °C (black bars) ( c ). * p
Figure Legend Snippet: Zeta potentials ( a ) and particle sizes (diameter) ( b ) of siRNA, siRNA with Tat/PEI, INT, and siRNA with mTat/PEI/INT. Effects of temperature on siRNA transfection efficiency with mTat/PEI, INT, or mTat/PEI/INT in HSC-3 cells at 4 °C (white bars) or 37 °C (black bars) ( c ). * p

Techniques Used: Transfection

Comparative transfection efficiency of FuGENE HD (FuGENE), X-tremeGENE (X-treme), SuperFect (Super), Lipofectamine 2000 (L-2000), Lipofectamine RNAiMAX (L-iMAX), INT, and mTat/PEI/INT using siRNA targeting β - actin in HSC-3 cells. β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( a ). Cell viability of each of the reagent groups in HaCaT cells were evaluated by MTT assay under the same siRNA and transfection reagent volume ( b ). * p
Figure Legend Snippet: Comparative transfection efficiency of FuGENE HD (FuGENE), X-tremeGENE (X-treme), SuperFect (Super), Lipofectamine 2000 (L-2000), Lipofectamine RNAiMAX (L-iMAX), INT, and mTat/PEI/INT using siRNA targeting β - actin in HSC-3 cells. β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( a ). Cell viability of each of the reagent groups in HaCaT cells were evaluated by MTT assay under the same siRNA and transfection reagent volume ( b ). * p

Techniques Used: Transfection, Quantitative RT-PCR, Expressing, MTT Assay

Related Articles

Transfection:

Article Title: Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway
Article Snippet: .. Non-viral vectors To compare transfection efficiency of siRNA targeting β -actin (Santa Cruz Biotechnology, Inc. Dallas, TX) with several commercial reagents, Lipofectamine 2000 (Invitrogen, Carlsbad, CA), which is a cationic lipid-based transfection reagent, FuGENE HD (Promega Corp., Fitchburg, WI), which is a blend of lipids and other components, SuperFect (Qiagen, Valencia, CA), which is an activated dendrimer-based reagent, Lipofectamine RNAiMAX (Invitrogen), which is a cationic lipid-based transfection reagent, X-tremeGENE (Roche, Switzerland), which is a blend of lipids and other components, and INT (Polyplus-transfection, France) were used. .. Transfections of siRNA into cells were performed according to each manufacturer’s instructions.

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    Promega cationic lipid based transfection reagent
    In vitro gene silencing efficiency of various concentrations of siRNA (0.1, 0.2, 0.4 and 0.8 ng/µl) with/without mTat/PEI, INT, and mTat/PEI/INT in HSC-3 cells. In vitro <t>transfection</t> efficiency of siRNA with/without mTat/PEI, INT (0.015, 0.03 and 0.06% v/v), and mTat/PEI/INT (0.015, 0.03, and 0.06% v/v) in HSC-3 cells ( a ). β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( b ). Transfection efficiency of siRNA with mTat/PEI/INT at the different concentration. siRNA concentration was 0.8 ng/µl ( c ). β-actin protein was measured in the cell lysate of HSC-3 cells transfected with siRNA with/without mTat/PEI, INT, and mTat/PEI/INT incubated for 48 h ( d ). siRNA concentration was 0.8 ng/µl and INT concentration was 0.06% v/v. Significant difference between INT/siRNA and mTat/PEI/INT/siRNA, * p
    Cationic Lipid Based Transfection Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cationic lipid based transfection reagent/product/Promega
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cationic lipid based transfection reagent - by Bioz Stars, 2020-08
    93/100 stars
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    In vitro gene silencing efficiency of various concentrations of siRNA (0.1, 0.2, 0.4 and 0.8 ng/µl) with/without mTat/PEI, INT, and mTat/PEI/INT in HSC-3 cells. In vitro transfection efficiency of siRNA with/without mTat/PEI, INT (0.015, 0.03 and 0.06% v/v), and mTat/PEI/INT (0.015, 0.03, and 0.06% v/v) in HSC-3 cells ( a ). β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( b ). Transfection efficiency of siRNA with mTat/PEI/INT at the different concentration. siRNA concentration was 0.8 ng/µl ( c ). β-actin protein was measured in the cell lysate of HSC-3 cells transfected with siRNA with/without mTat/PEI, INT, and mTat/PEI/INT incubated for 48 h ( d ). siRNA concentration was 0.8 ng/µl and INT concentration was 0.06% v/v. Significant difference between INT/siRNA and mTat/PEI/INT/siRNA, * p

    Journal: Journal of Nanobiotechnology

    Article Title: Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway

    doi: 10.1186/s12951-019-0444-8

    Figure Lengend Snippet: In vitro gene silencing efficiency of various concentrations of siRNA (0.1, 0.2, 0.4 and 0.8 ng/µl) with/without mTat/PEI, INT, and mTat/PEI/INT in HSC-3 cells. In vitro transfection efficiency of siRNA with/without mTat/PEI, INT (0.015, 0.03 and 0.06% v/v), and mTat/PEI/INT (0.015, 0.03, and 0.06% v/v) in HSC-3 cells ( a ). β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( b ). Transfection efficiency of siRNA with mTat/PEI/INT at the different concentration. siRNA concentration was 0.8 ng/µl ( c ). β-actin protein was measured in the cell lysate of HSC-3 cells transfected with siRNA with/without mTat/PEI, INT, and mTat/PEI/INT incubated for 48 h ( d ). siRNA concentration was 0.8 ng/µl and INT concentration was 0.06% v/v. Significant difference between INT/siRNA and mTat/PEI/INT/siRNA, * p

    Article Snippet: Non-viral vectors To compare transfection efficiency of siRNA targeting β -actin (Santa Cruz Biotechnology, Inc. Dallas, TX) with several commercial reagents, Lipofectamine 2000 (Invitrogen, Carlsbad, CA), which is a cationic lipid-based transfection reagent, FuGENE HD (Promega Corp., Fitchburg, WI), which is a blend of lipids and other components, SuperFect (Qiagen, Valencia, CA), which is an activated dendrimer-based reagent, Lipofectamine RNAiMAX (Invitrogen), which is a cationic lipid-based transfection reagent, X-tremeGENE (Roche, Switzerland), which is a blend of lipids and other components, and INT (Polyplus-transfection, France) were used.

    Techniques: In Vitro, Transfection, Quantitative RT-PCR, Expressing, Concentration Assay, Incubation

    Zeta potentials ( a ) and particle sizes (diameter) ( b ) of siRNA, siRNA with Tat/PEI, INT, and siRNA with mTat/PEI/INT. Effects of temperature on siRNA transfection efficiency with mTat/PEI, INT, or mTat/PEI/INT in HSC-3 cells at 4 °C (white bars) or 37 °C (black bars) ( c ). * p

    Journal: Journal of Nanobiotechnology

    Article Title: Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway

    doi: 10.1186/s12951-019-0444-8

    Figure Lengend Snippet: Zeta potentials ( a ) and particle sizes (diameter) ( b ) of siRNA, siRNA with Tat/PEI, INT, and siRNA with mTat/PEI/INT. Effects of temperature on siRNA transfection efficiency with mTat/PEI, INT, or mTat/PEI/INT in HSC-3 cells at 4 °C (white bars) or 37 °C (black bars) ( c ). * p

    Article Snippet: Non-viral vectors To compare transfection efficiency of siRNA targeting β -actin (Santa Cruz Biotechnology, Inc. Dallas, TX) with several commercial reagents, Lipofectamine 2000 (Invitrogen, Carlsbad, CA), which is a cationic lipid-based transfection reagent, FuGENE HD (Promega Corp., Fitchburg, WI), which is a blend of lipids and other components, SuperFect (Qiagen, Valencia, CA), which is an activated dendrimer-based reagent, Lipofectamine RNAiMAX (Invitrogen), which is a cationic lipid-based transfection reagent, X-tremeGENE (Roche, Switzerland), which is a blend of lipids and other components, and INT (Polyplus-transfection, France) were used.

    Techniques: Transfection

    Comparative transfection efficiency of FuGENE HD (FuGENE), X-tremeGENE (X-treme), SuperFect (Super), Lipofectamine 2000 (L-2000), Lipofectamine RNAiMAX (L-iMAX), INT, and mTat/PEI/INT using siRNA targeting β - actin in HSC-3 cells. β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( a ). Cell viability of each of the reagent groups in HaCaT cells were evaluated by MTT assay under the same siRNA and transfection reagent volume ( b ). * p

    Journal: Journal of Nanobiotechnology

    Article Title: Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway

    doi: 10.1186/s12951-019-0444-8

    Figure Lengend Snippet: Comparative transfection efficiency of FuGENE HD (FuGENE), X-tremeGENE (X-treme), SuperFect (Super), Lipofectamine 2000 (L-2000), Lipofectamine RNAiMAX (L-iMAX), INT, and mTat/PEI/INT using siRNA targeting β - actin in HSC-3 cells. β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( a ). Cell viability of each of the reagent groups in HaCaT cells were evaluated by MTT assay under the same siRNA and transfection reagent volume ( b ). * p

    Article Snippet: Non-viral vectors To compare transfection efficiency of siRNA targeting β -actin (Santa Cruz Biotechnology, Inc. Dallas, TX) with several commercial reagents, Lipofectamine 2000 (Invitrogen, Carlsbad, CA), which is a cationic lipid-based transfection reagent, FuGENE HD (Promega Corp., Fitchburg, WI), which is a blend of lipids and other components, SuperFect (Qiagen, Valencia, CA), which is an activated dendrimer-based reagent, Lipofectamine RNAiMAX (Invitrogen), which is a cationic lipid-based transfection reagent, X-tremeGENE (Roche, Switzerland), which is a blend of lipids and other components, and INT (Polyplus-transfection, France) were used.

    Techniques: Transfection, Quantitative RT-PCR, Expressing, MTT Assay