cation exchange proteinchip array (Bio-Rad)

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Bio-Rad cation exchange proteinchip array
Cation Exchange Proteinchip Array, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cation+exchange+proteinchip+array/ProteinChip+CM10+Arrays/us09459254-176-12-16
Average 93 stars, based on 91 article reviews

cation exchange proteinchip array - by Bioz Stars, 2026-09

93/100 stars

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Article Title: Method for determining sensitivity to an anticancer agent
Article Snippet: (a) ProteinChip Array and Buffer Conditions Employed in the Study For a cation-exchange ProteinChip array (CM10, Bio-Rad), the following 15 types of buffers were used: pH: 3.0 (50 mM glycine-HCl buffer), pH: 3.5 (50 mM sodium acetate buffer), pH: 4.0 (50 mM sodium acetate buffer), pH: 4.5 (50 mM sodium acetate buffer), pH: 5.0 (50 mM sodium acetate buffer), pH: 5.5 (50 mM sodium acetate buffer), pH: 6.0 (50 mM phosphate buffer), pH: 6.5 (50 mM phosphate buffer), pH: 7.0 (50 mM phosphate buffer), pH: 7.5 (50 mM phosphate buffer), pH: 8.0 (50 mM Tris-HCl buffer), pH: 8.5 (50 mM Tris-HCl buffer), pH: 9.0 (50 mM glycine-NaOH buffer), pH: 9.5 (50 mM glycine-NaOH buffer), and pH: 10.0 (50 mM glycine-NaOH buffer). (b) Preparation of Samples for CM10 Chip Array Analysis and Analysis Conditions Preparation of samples for CM10 chip array analysis, and production of protein chips were performed by use of buffers of (a) in accordance with (f) of method (1) of Example 1.

Article Title: Method for determination of sensitivity to anti-cancer agent
Article Snippet: The features of the peaks found in Example 1 were further investigated in terms of variation in peak intensity associated with change in pH. (1) Method (a) Proteinchip Array and Buffer Conditions Employed in the Study For a cation-exchange ProteinChip array (CM10, Bio-Rad), the following 15 types of buffers were used: pH: 3.0 (50 mM glycine-HCl buffer), pH: 3.5 (50 mM sodium acetate buffer), pH: 4.0 (50 mM sodium acetate buffer), pH: 4.5 (50 mM sodium acetate buffer), pH: 5.0 (50 mM sodium acetate buffer), pH: 5.5 (50 mM sodium acetate buffer), pH: 6.0 (50 mM phosphate buffer), pH: 6.5 (50 mM phosphate buffer), pH: 7.0 (50 mM phosphate buffer), pH: 7.5 (50 mM phosphate buffer), pH: 8.0 (50 mM Tris-HCl buffer), pH: 8.5 (50 mM Tris-HCl buffer), pH: 9.0 (50 mM glycine-NaOH buffer), pH: 9.5 (50 mM glycine-NaOH buffer), and pH: 10.0 (50 mM glycine-NaOH buffer). (b) Preparation of Samples for CM10 Chip Array Analysis and Analysis Conditions Preparation of samples for CM10 chip array analysis, and production of protein chips were performed by use of buffers of (a) in accordance with (f) of method (1) of Example 1. (2) Results In the CM10 chip array analysis, the pH at which the relevant peak intensity drops is thought to be the isoelectric point (pI) of the protein, where an ionized form is neutralized.

Incubation:

Article Title: Method for determination of sensitivity to anti-cancer agent
Article Snippet: A part of the supernatant was used to perform protein quantification (DC Protein Assay Kit, Bio-Rad). (e) Sample Preparation for Protein Expression Analysis with ProteinChip and Expression Analysis of Intracellular Protein A sample was adjusted to have a protein concentration of 5 mg/mL with a cell lysis buffer (excluding protease inhibitor) and then adjusted to have a concentration of 1 mg/mL with a dilution/wash buffer (50 mM sodium acetate buffer) (hereinafter, referred to as buffer) having a pH of 4.5. .. 100 μl of the adjusted sample were applied to each spot of a cation-exchange Proteinchip array (CM10, Bio-Rad) which had been pretreated with the same buffer, followed by reaction by incubation for 1 hour. ..

Article Title: Alterations in Cerebrospinal Fluid Proteins in a Presymptomatic Primary Glioma Model
Article Snippet: .. An aliquot of denatured CSF (10 μl) was then added to 90 μl 0.1 M acetate buffer, pH 4, and incubated on a negatively charged weak cation exchange ProteinChip array (CM10, Bio-Rad, Hercules, CA). ..

Clinical Proteomics:

Modification:

Article Title: Method for determination of sensitivity to anti-cancer agent
Article Snippet: .. In order to study the property of the protein detected as a peak at m/z of 11,000 to 11,100 (protein A1) in Example 1, there were examined change in the peak intensity associated with change in pH, and whether the protein A1 could be detected or not in a ProteinChip array whose chip surface was subjected to a chemical modification different from that of a CM10 chip array. (1) Method (a) Proteinchip Array and Buffer Conditions Used for Examination For the cation-exchange ProteinChip array (CM10, Bio-Rad) and the anion-exchange ProteinChip array (Q10, Bio-Rad), the following 15 kinds of buffers were used: pH3.0 (50 mM glycine-HCl buffer); pH 3.5 (50 mM sodium acetate buffer); pH 4.0 (50 mM sodium acetate buffer); pH 4.5 (50 mM sodium acetate buffer); pH 5.0 (50 mM sodium acetate buffer); pH 5.5 (50 mM sodium acetate buffer); pH 6.0 (50 mM phosphate buffer); pH 6.5 (50 mM phosphate buffer); pH 7.0 (50 mM phosphate buffer); pH 7.5 (50 mM phosphate buffer); pH 8.0 (50 mM Tris-HCl buffer); pH 8.5 (50 mM Tris-HCl buffer); pH 9.0 (50 mM glycine-NaOH buffer); pH 9.5 (50 mM glycine-NaOH buffer); and pH 10.0 (50 mM glycine-NaOH buffer). .. For the immobilized metal affinity capture ProteinChip array (IMAC30, Bio-Rad), phosphate buffered saline (PBS) was used. (b) Sample Preparation for Analysis Using CM10 and Q10 Arrays and Analysis Condition The sample preparation for analysis using CM10 or Q10 arrays and production of protein chip arrays were conducted using each buffer in the item (a) and according to the item (e) of the section “(1) Method” in Example 1.

Binding Assay:

Article Title: Mass spectrometry measurement of plasma hepcidin for the prediction of iron overload
Article Snippet: Background: Hepcidin has emerged as the primary regulator of iron homeostasis.. Previous studies on assessing urinary hepcidin are limited.. We developed a method for quantifying hepcidin-25 (Hep-25) in plasma using surface-enhanced laser-desorption-ionization time-of-flight mass spectrometry (SELDI-TOF/MS) and a 25-AA peptide as reference standard.

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