Journal: Poultry Science

Article Title: Effect of selenium-enriched Ganoderma lucidum powder in testicular tissues, oxidative stress biomarkers and antiapoptotic capacities in roosters

doi: 10.1016/j.psj.2025.106185

Figure Lengend Snippet: SeGL attenuates the level of apoptosis in the rooster testes. A–B . Relative mRNA expression of Bax, Bcl-2, Caspase3 and the Bax/Bcl-2 ratio in the testes of the control, LSe, MSe, or HSe group (n = 3). C . Western blot of Bax, Bcl-2, and Caspase3 proteins in the testes of each group. D–E . Quantitative analysis of Bax, Bcl-2, Caspase3 protein expression and the Bax/Bcl-2 ratio in the testes of each group (n = 3). Data are presented as means ± SD; different lowercase letters indicate significant differences between groups (P < 0.05).

Article Snippet: After blocking with 5 % non-fat milk (A600669, Sangon, Shanghai, China) in TBST (Tris-buffered saline with 0.1 % Tween-20) for 1 h at room temperature, membranes were incubated overnight at 4°C with primary antibodies against target proteins [Bax (50599-2-lg, Proteintech, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Caspase3 (PB9188, Boster, Wuhan, China), NF-κB (PB9149, Boster, Wuhan, China), p53 (PB9008, Boster, Wuhan, China), StAR (A00051-1, Boster, Wuhan, China), Cyp11a1 (BA3699, Boster, Wuhan, China), Hsd3b1 (A02856-2, Boster, Wuhan, China)] and β-tubulin (internal control; bsm-33034 M, Bioss Antibodies, Beijing, China).

Techniques: Expressing, Control, Western Blot