anti caspase 3 antibody  (Abcam)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Name:
    Anti Caspase 3 antibody
    Description:

    Catalog Number:
    AB13847
    Price:
    None
    Buy from Supplier


    Structured Review

    Abcam anti caspase 3 antibody
    a. <t>Caspase-3</t> immunostaining (control group). The control group caspase-3 results showed that caspase-3 expression was negative in germinal epithelial cells, granular cells of preantral and antral follicles, and endothelial cells. However, it was positive in the stromal cells around the follicle. b. Caspase-3 immunostaining (ischemia group). The positive expression of caspase-3 was observed in degenerated granular cells of preantral and antral follicles, luteal cells of the corpus luteum, and many inflammatory cells in the stromal region ( red arrow ). c. Caspase-3 immunostaining (ischemia-reperfusion group). Caspase-3 was positively expressed in granular cells in mature antral follicles and inflammatory cells in the stromal region ( red arrow ). d. Caspase-3 immunostaining (ischemia-reperfusion + RA group). Caspase-3 expression was negative in the preantral follicle cells and granular cells around the antral follicle ( red arrow ), whereas it was positive in some stromal cells and corpus luteum cells. Scale bar = 50 μm.

    https://www.bioz.com/result/anti caspase 3 antibody/product/Abcam
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti caspase 3 antibody - by Bioz Stars, 2021-05
    97/100 stars

    Images

    1) Product Images from "Investigation of the role of rosmarinic acid treatment in regulating inflammation, cell damage, and angiogenesis in rat ovarian torsion and detorsion models 1"

    Article Title: Investigation of the role of rosmarinic acid treatment in regulating inflammation, cell damage, and angiogenesis in rat ovarian torsion and detorsion models 1

    Journal: Acta Cirúrgica Brasileira

    doi: 10.1590/s0102-865020200030000004

    a. Caspase-3 immunostaining (control group). The control group caspase-3 results showed that caspase-3 expression was negative in germinal epithelial cells, granular cells of preantral and antral follicles, and endothelial cells. However, it was positive in the stromal cells around the follicle. b. Caspase-3 immunostaining (ischemia group). The positive expression of caspase-3 was observed in degenerated granular cells of preantral and antral follicles, luteal cells of the corpus luteum, and many inflammatory cells in the stromal region ( red arrow ). c. Caspase-3 immunostaining (ischemia-reperfusion group). Caspase-3 was positively expressed in granular cells in mature antral follicles and inflammatory cells in the stromal region ( red arrow ). d. Caspase-3 immunostaining (ischemia-reperfusion + RA group). Caspase-3 expression was negative in the preantral follicle cells and granular cells around the antral follicle ( red arrow ), whereas it was positive in some stromal cells and corpus luteum cells. Scale bar = 50 μm.
    Figure Legend Snippet: a. Caspase-3 immunostaining (control group). The control group caspase-3 results showed that caspase-3 expression was negative in germinal epithelial cells, granular cells of preantral and antral follicles, and endothelial cells. However, it was positive in the stromal cells around the follicle. b. Caspase-3 immunostaining (ischemia group). The positive expression of caspase-3 was observed in degenerated granular cells of preantral and antral follicles, luteal cells of the corpus luteum, and many inflammatory cells in the stromal region ( red arrow ). c. Caspase-3 immunostaining (ischemia-reperfusion group). Caspase-3 was positively expressed in granular cells in mature antral follicles and inflammatory cells in the stromal region ( red arrow ). d. Caspase-3 immunostaining (ischemia-reperfusion + RA group). Caspase-3 expression was negative in the preantral follicle cells and granular cells around the antral follicle ( red arrow ), whereas it was positive in some stromal cells and corpus luteum cells. Scale bar = 50 μm.

    Techniques Used: Immunostaining, Expressing

    2) Product Images from "Sulforaphane regulates apoptosis- and proliferation-related signaling pathways and synergizes with cisplatin to suppress human ovarian cancer"

    Article Title: Sulforaphane regulates apoptosis- and proliferation-related signaling pathways and synergizes with cisplatin to suppress human ovarian cancer

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2018.3860

    Sulforaphane synergizes with cisplatin in suppressing cancer cell proliferation and promoting apoptosis of ovarian cancer cells. (A) Synergism between sulforaphane and cisplatin. A2780 cells were treated with sulforaphane and cisplatin at the indicated concentrations. After 24 h, WST-1 reagent was administrated to the culture medium and then incubated for 1 h. The WST-1 activities were determined at 440 nm. (B) Colony formation assay. Subconfluent A2780 cells were seeded in plates and treated with sulforaphane and cisplatin at the indicated concentrations. After 48 h, the cancer cells were then fixed and stained with crystal violet for the colony formation assay. (C) Colony-forming ability of ovarian cancer cells following sulforaphane and cisplatin treatment was quantified for optical absorbance. (D) Western blot assays with (E) quantification were performed to determine protein levels of Bcl-2, Caspase-3, P53, and Cyclin-D1 in A2780 cells treated with or without sulforaphane and cisplatin. The values are presented as the mean ± standard deviation (n=8-10) of the samples. + P
    Figure Legend Snippet: Sulforaphane synergizes with cisplatin in suppressing cancer cell proliferation and promoting apoptosis of ovarian cancer cells. (A) Synergism between sulforaphane and cisplatin. A2780 cells were treated with sulforaphane and cisplatin at the indicated concentrations. After 24 h, WST-1 reagent was administrated to the culture medium and then incubated for 1 h. The WST-1 activities were determined at 440 nm. (B) Colony formation assay. Subconfluent A2780 cells were seeded in plates and treated with sulforaphane and cisplatin at the indicated concentrations. After 48 h, the cancer cells were then fixed and stained with crystal violet for the colony formation assay. (C) Colony-forming ability of ovarian cancer cells following sulforaphane and cisplatin treatment was quantified for optical absorbance. (D) Western blot assays with (E) quantification were performed to determine protein levels of Bcl-2, Caspase-3, P53, and Cyclin-D1 in A2780 cells treated with or without sulforaphane and cisplatin. The values are presented as the mean ± standard deviation (n=8-10) of the samples. + P

    Techniques Used: Incubation, Colony Assay, Staining, Western Blot, Standard Deviation

    Sulforaphane inhibits multiple cancer development-associated signaling pathways in A2780 cells. (A) Effect of sulforaphane on intracellular levels of Her2 through immunofluorescence assays (magnification, ×100). (B) Western blot assays were performed to examine the effect of sulforaphane on the 10 cancer-associated signaling pathway reporter activities, including Bcl-2, Bax, Cyto-c, Caspase-3, p-AKT, p-NF-κB, P53, P27, cyclin-D1 and cMyc. The values are presented as the mean ± standard deviation (n=8-10) of the samples. + P
    Figure Legend Snippet: Sulforaphane inhibits multiple cancer development-associated signaling pathways in A2780 cells. (A) Effect of sulforaphane on intracellular levels of Her2 through immunofluorescence assays (magnification, ×100). (B) Western blot assays were performed to examine the effect of sulforaphane on the 10 cancer-associated signaling pathway reporter activities, including Bcl-2, Bax, Cyto-c, Caspase-3, p-AKT, p-NF-κB, P53, P27, cyclin-D1 and cMyc. The values are presented as the mean ± standard deviation (n=8-10) of the samples. + P

    Techniques Used: Immunofluorescence, Western Blot, Standard Deviation

    3) Product Images from "Improved Vascular Survival and Growth in the Mouse Model of Hindlimb Ischemia by a Remote Signaling Mechanism"

    Article Title: Improved Vascular Survival and Growth in the Mouse Model of Hindlimb Ischemia by a Remote Signaling Mechanism

    Journal: The American Journal of Pathology

    doi: 10.1016/j.ajpath.2013.11.032

    Reduced ischemia injury and improved arterial growth in thigh muscles of k Phd2 KO mice. A and B: Detection of apoptotic myocytes (brown) by anti–activated caspase 3 IHC staining. Adductor muscle cross-sections used in this assay were prepared 3
    Figure Legend Snippet: Reduced ischemia injury and improved arterial growth in thigh muscles of k Phd2 KO mice. A and B: Detection of apoptotic myocytes (brown) by anti–activated caspase 3 IHC staining. Adductor muscle cross-sections used in this assay were prepared 3

    Techniques Used: Mouse Assay, Immunohistochemistry, Staining

    4) Product Images from "Subcutaneous Administration of PDGF-AA Improves the Functional Recovery After Spinal Cord Injury"

    Article Title: Subcutaneous Administration of PDGF-AA Improves the Functional Recovery After Spinal Cord Injury

    Journal: Frontiers in Neuroscience

    doi: 10.3389/fnins.2019.00006

    Survival of OLs in the injured spinal cord after SCI, as determined by activated caspase-3 staining. (A,B) Representative photomicrographs showing caspase-3+cells (red) co-localized with CNP+OLs (green) in the circumambience of the injured spinal cord of rats at 2 weeks post-SCI. (C,D) Quantitative analysis of OL survival showing that both the number and percentage of caspase-3+OLs were higher in rats that received PDGF-AA versus controls. Data represent the mean ± SD ( n = 8). ∗ P
    Figure Legend Snippet: Survival of OLs in the injured spinal cord after SCI, as determined by activated caspase-3 staining. (A,B) Representative photomicrographs showing caspase-3+cells (red) co-localized with CNP+OLs (green) in the circumambience of the injured spinal cord of rats at 2 weeks post-SCI. (C,D) Quantitative analysis of OL survival showing that both the number and percentage of caspase-3+OLs were higher in rats that received PDGF-AA versus controls. Data represent the mean ± SD ( n = 8). ∗ P

    Techniques Used: Staining

    Survival of OPCs in the injured spinal cord after SCI, as determined by activated caspase-3 staining. (A,B) Representative photomicrographs showing caspase-3+cells (red) co-localized with NG2+OPCs (green) in the circumambience of the injured spinal cord of rats at 2 weeks post-SCI. (C,D) Quantitative analysis of OPC survival showed that both the number and percentage of caspase-3+ OPCs were higher in rats that received PDGF-AA versus controls. Data represent the mean ± SD ( n = 8). ∗ P
    Figure Legend Snippet: Survival of OPCs in the injured spinal cord after SCI, as determined by activated caspase-3 staining. (A,B) Representative photomicrographs showing caspase-3+cells (red) co-localized with NG2+OPCs (green) in the circumambience of the injured spinal cord of rats at 2 weeks post-SCI. (C,D) Quantitative analysis of OPC survival showed that both the number and percentage of caspase-3+ OPCs were higher in rats that received PDGF-AA versus controls. Data represent the mean ± SD ( n = 8). ∗ P

    Techniques Used: Staining

    5) Product Images from "CCL2 and IL18 expressions may associate with the anti-proliferative effect of noncontact electro capacitive cancer therapyin vivo"

    Article Title: CCL2 and IL18 expressions may associate with the anti-proliferative effect of noncontact electro capacitive cancer therapyin vivo

    Journal: F1000Research

    doi: 10.12688/f1000research.20727.1

    Immunostaining of breast adenocarcinoma tissue sections after ECCT treatment. ( A and B ) Anti-PCNA, ( C and D ) anti-ErbB2, ( E and F ) anti-Caspase-3, ( G and H ) anti-CD68. Percentage of positive cells of ( I ) PCNA, ( J ) ErbB2, and ( K ) Caspase-3 in 50 fields of view. ( L ) Count of total macrophages in 50 fields of view. Observation of histological slide was performed using Leica CC50 E at 0.5 µm/pixel resolution at 400x. Bar=100 µm. The mean, standard deviation of the data experiment show *, p
    Figure Legend Snippet: Immunostaining of breast adenocarcinoma tissue sections after ECCT treatment. ( A and B ) Anti-PCNA, ( C and D ) anti-ErbB2, ( E and F ) anti-Caspase-3, ( G and H ) anti-CD68. Percentage of positive cells of ( I ) PCNA, ( J ) ErbB2, and ( K ) Caspase-3 in 50 fields of view. ( L ) Count of total macrophages in 50 fields of view. Observation of histological slide was performed using Leica CC50 E at 0.5 µm/pixel resolution at 400x. Bar=100 µm. The mean, standard deviation of the data experiment show *, p

    Techniques Used: Immunostaining, Standard Deviation

    6) Product Images from "An ATF24 peptide-functionalized β-elemene-nanostructured lipid carrier combined with cisplatin for bladder cancer treatment"

    Article Title: An ATF24 peptide-functionalized β-elemene-nanostructured lipid carrier combined with cisplatin for bladder cancer treatment

    Journal: Cancer Biology & Medicine

    doi: 10.20892/j.issn.2095-3941.2020.0454

    The safety of various formulations and the tumor progression level were evaluated by hematoxylin and eosin (H E) staining and immunohistochemical staining. (A) H E staining of the 3 organs including the liver, spleen, and kidneys (20×). (B) Tumor tissues were evaluated qualitatively to detect the urokinase-type plasminogen activator receptor, Ki-67, and cleaved caspase-3 expression using immunohistochemistry (20×).
    Figure Legend Snippet: The safety of various formulations and the tumor progression level were evaluated by hematoxylin and eosin (H E) staining and immunohistochemical staining. (A) H E staining of the 3 organs including the liver, spleen, and kidneys (20×). (B) Tumor tissues were evaluated qualitatively to detect the urokinase-type plasminogen activator receptor, Ki-67, and cleaved caspase-3 expression using immunohistochemistry (20×).

    Techniques Used: Staining, Immunohistochemistry, Expressing

    7) Product Images from "Protective Effects of Total Saponins of Aralia elata (Miq.) on Endothelial Cell Injury Induced by TNF-α via Modulation of the PI3K/Akt and NF-κB Signalling Pathways"

    Article Title: Protective Effects of Total Saponins of Aralia elata (Miq.) on Endothelial Cell Injury Induced by TNF-α via Modulation of the PI3K/Akt and NF-κB Signalling Pathways

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms20010036

    TAS suppressed TNF-α-induced apoptosis . HUVECs were preincubated with TAS (5 μg/mL) for 2 h, followed by treatment with TNF-α (50 ng/mL) for 24 h. HUVEC apoptosis was assessed by ( A ) TUNEL staining and ( B ) annexin V/propidium iodide (PI) staining. ( C ) Quantitative analysis of the ratio of TUNEL-positive cells. ( D ) Quantitative analysis of the ratio of apoptotic cells. ( E ) Cells were preincubated with TAS (5 μg/mL) for 2 h, followed by treatment with TNF-α for 24 h. Caspase-3 activity was examined with a fluorescent labelling kit using a microplate reader. The data are expressed as the mean ± SD of three independent experiments. ## p
    Figure Legend Snippet: TAS suppressed TNF-α-induced apoptosis . HUVECs were preincubated with TAS (5 μg/mL) for 2 h, followed by treatment with TNF-α (50 ng/mL) for 24 h. HUVEC apoptosis was assessed by ( A ) TUNEL staining and ( B ) annexin V/propidium iodide (PI) staining. ( C ) Quantitative analysis of the ratio of TUNEL-positive cells. ( D ) Quantitative analysis of the ratio of apoptotic cells. ( E ) Cells were preincubated with TAS (5 μg/mL) for 2 h, followed by treatment with TNF-α for 24 h. Caspase-3 activity was examined with a fluorescent labelling kit using a microplate reader. The data are expressed as the mean ± SD of three independent experiments. ## p

    Techniques Used: TUNEL Assay, Staining, Activity Assay

    8) Product Images from "Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide-induced acute pneumonia in rats"

    Article Title: Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide-induced acute pneumonia in rats

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2020.11410

    Anti-apoptotic effect of UCF-101 in pneumonia. The condition of apoptosis was detected and (A) the amount of apoptotic cells in lung tissue was determined by TUNEL assay. Magnification, ×400. The expression of apoptosis-related proteins (B) Bcl-2, (C) Bax, (D) cleaved caspase-3, and (E) cleaved caspase-9 were determined using western blotting. Data are expressed as mean ± standard deviation (n=8). ***P
    Figure Legend Snippet: Anti-apoptotic effect of UCF-101 in pneumonia. The condition of apoptosis was detected and (A) the amount of apoptotic cells in lung tissue was determined by TUNEL assay. Magnification, ×400. The expression of apoptosis-related proteins (B) Bcl-2, (C) Bax, (D) cleaved caspase-3, and (E) cleaved caspase-9 were determined using western blotting. Data are expressed as mean ± standard deviation (n=8). ***P

    Techniques Used: TUNEL Assay, Expressing, Western Blot, Standard Deviation

    9) Product Images from "P4HB knockdown induces human HT29 colon cancer cell apoptosis through the generation of reactive oxygen species and inactivation of STAT3 signaling"

    Article Title: P4HB knockdown induces human HT29 colon cancer cell apoptosis through the generation of reactive oxygen species and inactivation of STAT3 signaling

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2018.9660

    Effects of P4HB knockdown on markers of apoptosis. (A) Representative images of the protein levels of p53, Bcl-2, cleaved caspase-3, p-STAT3, t-STAT3 and c-Myc as assessed by western blot analysis. (B) P4HB knockdown increased the generation of ROS. Images are representative of three independent experiments. Data are presented as the means ± standard deviation. *P
    Figure Legend Snippet: Effects of P4HB knockdown on markers of apoptosis. (A) Representative images of the protein levels of p53, Bcl-2, cleaved caspase-3, p-STAT3, t-STAT3 and c-Myc as assessed by western blot analysis. (B) P4HB knockdown increased the generation of ROS. Images are representative of three independent experiments. Data are presented as the means ± standard deviation. *P

    Techniques Used: Western Blot, Standard Deviation

    Inhibiting accumulation of ROS reduces apoptosis induced by P4HB knockdown. (A) ROS generation, as measured by flow cytometry. (B and C) Protein levels of Bcl-2, cleaved caspase-3, p-STAT3 and t-STAT3 were assessed by western blot analyses. (D) Annexin V/PI staining was performed to assess cell apoptosis. Images are representative of three independent experiments. All data are presented as the means ± standard deviation. *P
    Figure Legend Snippet: Inhibiting accumulation of ROS reduces apoptosis induced by P4HB knockdown. (A) ROS generation, as measured by flow cytometry. (B and C) Protein levels of Bcl-2, cleaved caspase-3, p-STAT3 and t-STAT3 were assessed by western blot analyses. (D) Annexin V/PI staining was performed to assess cell apoptosis. Images are representative of three independent experiments. All data are presented as the means ± standard deviation. *P

    Techniques Used: Flow Cytometry, Cytometry, Western Blot, Staining, Standard Deviation

    Related Articles

    Staining:

    Article Title: Improved Vascular Survival and Growth in the Mouse Model of Hindlimb Ischemia by a Remote Signaling Mechanism
    Article Snippet: Signals were detected by staining with biotinylated secondary antibodies in conjunction with avidin-biotin complex and diaminobenzidine kits (Vector Laboratories, Burlingame, CA). .. For IF staining, sections were incubated with each of the following pairs of primary and secondary antibodies: anti-CD31 (Santa Cruz Biotechnology; sc-1506) and Alexa Fluor 488–conjugated anti-goat IgG (Life Technologies, Carlsbad, CA; A-11055), anti-Ki67 (BioLegend; number 652401) and cyanine 3–conjugated anti-rat IgG (Jackson ImmunoResearch Laboratories Inc., West Grove, PA; 712-166-153), and anti–activated caspase 3 (Abcam, Cambridge, MA; ab13847) and Alexa Fluor 594–conjugated anti-rabbit IgG (Life Technologies; A-11072). .. IHC- or IF-stained sections were visualized by bright field light microscopy or laser confocal microscopy, respectively, and quantified with the assistance of ImageJ software version 1.46e (NIH, Bethesda, MD).

    Incubation:

    Article Title: Improved Vascular Survival and Growth in the Mouse Model of Hindlimb Ischemia by a Remote Signaling Mechanism
    Article Snippet: Signals were detected by staining with biotinylated secondary antibodies in conjunction with avidin-biotin complex and diaminobenzidine kits (Vector Laboratories, Burlingame, CA). .. For IF staining, sections were incubated with each of the following pairs of primary and secondary antibodies: anti-CD31 (Santa Cruz Biotechnology; sc-1506) and Alexa Fluor 488–conjugated anti-goat IgG (Life Technologies, Carlsbad, CA; A-11055), anti-Ki67 (BioLegend; number 652401) and cyanine 3–conjugated anti-rat IgG (Jackson ImmunoResearch Laboratories Inc., West Grove, PA; 712-166-153), and anti–activated caspase 3 (Abcam, Cambridge, MA; ab13847) and Alexa Fluor 594–conjugated anti-rabbit IgG (Life Technologies; A-11072). .. IHC- or IF-stained sections were visualized by bright field light microscopy or laser confocal microscopy, respectively, and quantified with the assistance of ImageJ software version 1.46e (NIH, Bethesda, MD).

    Article Title: An ATF24 peptide-functionalized β-elemene-nanostructured lipid carrier combined with cisplatin for bladder cancer treatment
    Article Snippet: Western blot analysis The cells were treated with ATF24 -PEG-Lipo-β-E and/or DDP for the indicated times. .. The PVDF membranes were incubated with anti-cyclin B1 (1:1,000, Cell Signaling Technology, anti-Bcl-2 (1:1,000; Cell Signaling Technology), anti-Bax (1:1,000; Cell Signaling Technology), anti-cleaved PARP (1:1,000; Cell Signaling Technology), anti-Cdc25C (1:200; Santa Cruz Biotechnology, Dallas, TX, USA), anti-Cdc2 p34 (1:200; Santa Cruz Biotechnology), anti-cleaved caspase-3 (1:400; Abcam, Cambridge, UK), and anti-GAPDH (1:1,000; Cell Signaling Technology) primary antibodies at 4 °C overnight. .. In vivo antitumor efficacy The antitumor efficacy of ATF24 -PEG-Lipo-β-E and the combined treatment of ATF24 -PEG-Lipo-β-E and DDP injection were evaluated in tumor-bearing xenografts.

    Article Title: CCL2 and IL18 expressions may associate with the anti-proliferative effect of noncontact electro capacitive cancer therapyin vivo
    Article Snippet: The samples were soaked with 3% H2 O2 (Sigma-Aldrich) in PBS for 5 min to block endogenous peroxidase and subsequently treated with Background Sniper for 20 minutes for suppressing nonspecific binding. .. Afterwards, samples were separately incubated with anti-PCNA (ABCAM; cat.no. ab18197), anti-caspase-3 (ABCAM; cat.no. ab13847), anti-CD68 (ABCAM; cat.no. ab201340), and anti-ErbB 2 (ABCAM; cat.no. ab16901) antibodies overnight at 4 °C, followed by Trekkie Universal Link incubation for 60 minutes. .. Then, the samples were incubated with Trek-avidin HRP Label for staining development and then counterstained with hematoxylin.

    Article Title: Investigation of the role of rosmarinic acid treatment in regulating inflammation, cell damage, and angiogenesis in rat ovarian torsion and detorsion models 1
    Article Snippet: To unmask antigen sites, the slides were incubated with EDTA solution in a microwave for 110 minutes at 3 x 90oC. .. The sections were washed three times for 5 min in PBS and were incubated with hydrogen peroxide (catalogue # TA-015-HP, Thermo Fisher Scientific, US) for 20 min. Ultra V block (TA-125-UB, Thermo Fisher Scientific, US) was applied to the sections for 8 min prior to the addition of the primary antibodies, which were left on overnight in VEGF antibody (catalogue # ab1316, 1:100), TNF-α antibody (catalogue # ab6671, 1:100), and caspase-3 antibody (catalogue # ab4051, 1:100), all from Abcam, US. .. The sections were washed three times for 5 min in PBS and then were incubated with biotinylated secondary antibody (catalogue # TP-125-BN, Thermo Fisher Scientific, US) for 14 min. After washing with PBS, streptavidin peroxidase (catalogue # TS-125-HR, Thermo Fisher Scientific, US) was applied to the sections for 15 min.

    Blocking Assay:

    Article Title: Investigation of the role of rosmarinic acid treatment in regulating inflammation, cell damage, and angiogenesis in rat ovarian torsion and detorsion models 1
    Article Snippet: To unmask antigen sites, the slides were incubated with EDTA solution in a microwave for 110 minutes at 3 x 90oC. .. The sections were washed three times for 5 min in PBS and were incubated with hydrogen peroxide (catalogue # TA-015-HP, Thermo Fisher Scientific, US) for 20 min. Ultra V block (TA-125-UB, Thermo Fisher Scientific, US) was applied to the sections for 8 min prior to the addition of the primary antibodies, which were left on overnight in VEGF antibody (catalogue # ab1316, 1:100), TNF-α antibody (catalogue # ab6671, 1:100), and caspase-3 antibody (catalogue # ab4051, 1:100), all from Abcam, US. .. The sections were washed three times for 5 min in PBS and then were incubated with biotinylated secondary antibody (catalogue # TP-125-BN, Thermo Fisher Scientific, US) for 14 min. After washing with PBS, streptavidin peroxidase (catalogue # TS-125-HR, Thermo Fisher Scientific, US) was applied to the sections for 15 min.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Abcam anti active caspase 3 antibody
    Knockdown VEGFA inhibited autophagy and increased apoptosis. ( A ) Western blotting analysis of autophagy biomarkers LC3I-II, Beclin-1 and p62 in SKOV3 and SKOV3/DDP cells. ( B ) Western blotting analysis of Beclin-1 and LC3I-II in SKVOK3 cells treated with DDP (50µM), PTX (40µM), and ADM (20µM) for 48 hours. ( C ) Western blotting analysis of autophagy biomarkers LC3I-II and p62 in SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. ( D ) Western blotting analysis of apoptotic biomarkers <t>caspase-3</t> and cleaved caspase-3 in SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. ( E ) Flow cytometry analysis of apoptosis of SKOV3/DDP cells after transfected with shVEGFA or control treated with 3-MA. ( F ) Statistical analysis of apoptosis ratio of SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. Results are representative of three independent experiments. ** P
    Anti Active Caspase 3 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti active caspase 3 antibody/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti active caspase 3 antibody - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    95
    Abcam rabbit anti lmo4
    Growth pattern of <t>LMO4</t> knockout UB/OC1 cells. A, Growth curve of the cells, determined by using MTT calorimetric assay, indicated that the growth rate of the LMO4 knockout cells is similar to that of the control cells during the initial 4 days and is relatively slower on day 5. The results are expressed as mean ± standard deviation, n = 6. B, Representative image of LMO4 knockout UB/OC1 cell colonies, 2 weeks after plating, indicates the survival potential of the cells and their ability to form colonies. The image is representative of three biological replicates.
    Rabbit Anti Lmo4, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti lmo4/product/Abcam
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti lmo4 - by Bioz Stars, 2021-05
    95/100 stars
      Buy from Supplier


    Image Search Results


    Knockdown VEGFA inhibited autophagy and increased apoptosis. ( A ) Western blotting analysis of autophagy biomarkers LC3I-II, Beclin-1 and p62 in SKOV3 and SKOV3/DDP cells. ( B ) Western blotting analysis of Beclin-1 and LC3I-II in SKVOK3 cells treated with DDP (50µM), PTX (40µM), and ADM (20µM) for 48 hours. ( C ) Western blotting analysis of autophagy biomarkers LC3I-II and p62 in SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. ( D ) Western blotting analysis of apoptotic biomarkers caspase-3 and cleaved caspase-3 in SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. ( E ) Flow cytometry analysis of apoptosis of SKOV3/DDP cells after transfected with shVEGFA or control treated with 3-MA. ( F ) Statistical analysis of apoptosis ratio of SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. Results are representative of three independent experiments. ** P

    Journal: OncoTargets and therapy

    Article Title: Inhibition of VEGFA Increases the Sensitivity of Ovarian Cancer Cells to Chemotherapy by Suppressing VEGFA-Mediated Autophagy

    doi: 10.2147/OTT.S250392

    Figure Lengend Snippet: Knockdown VEGFA inhibited autophagy and increased apoptosis. ( A ) Western blotting analysis of autophagy biomarkers LC3I-II, Beclin-1 and p62 in SKOV3 and SKOV3/DDP cells. ( B ) Western blotting analysis of Beclin-1 and LC3I-II in SKVOK3 cells treated with DDP (50µM), PTX (40µM), and ADM (20µM) for 48 hours. ( C ) Western blotting analysis of autophagy biomarkers LC3I-II and p62 in SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. ( D ) Western blotting analysis of apoptotic biomarkers caspase-3 and cleaved caspase-3 in SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. ( E ) Flow cytometry analysis of apoptosis of SKOV3/DDP cells after transfected with shVEGFA or control treated with 3-MA. ( F ) Statistical analysis of apoptosis ratio of SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. Results are representative of three independent experiments. ** P

    Article Snippet: AntibodiesAnti-VEGFA (ab1316), anti-caspase-3 (ab179517) antibodies, and anti-cleaved caspase-3 (ab2302) were purchased from Abcam (USA).

    Techniques: Western Blot, Transfection, Flow Cytometry

    Growth pattern of LMO4 knockout UB/OC1 cells. A, Growth curve of the cells, determined by using MTT calorimetric assay, indicated that the growth rate of the LMO4 knockout cells is similar to that of the control cells during the initial 4 days and is relatively slower on day 5. The results are expressed as mean ± standard deviation, n = 6. B, Representative image of LMO4 knockout UB/OC1 cell colonies, 2 weeks after plating, indicates the survival potential of the cells and their ability to form colonies. The image is representative of three biological replicates.

    Journal: Journal of cellular biochemistry

    Article Title: CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth

    doi: 10.1002/jcb.26529

    Figure Lengend Snippet: Growth pattern of LMO4 knockout UB/OC1 cells. A, Growth curve of the cells, determined by using MTT calorimetric assay, indicated that the growth rate of the LMO4 knockout cells is similar to that of the control cells during the initial 4 days and is relatively slower on day 5. The results are expressed as mean ± standard deviation, n = 6. B, Representative image of LMO4 knockout UB/OC1 cell colonies, 2 weeks after plating, indicates the survival potential of the cells and their ability to form colonies. The image is representative of three biological replicates.

    Article Snippet: The separated proteins were transferred on to apolyvinylidene difluoride membrane, blocked with 5% fat-free milk in tris-buffered saline containing 0.05% Tween 20, and incubated overnight with rabbit anti-LMO4 (1:1000, ab39383, Abcam, Cambridge, MA).

    Techniques: Knock-Out, MTT Assay, Standard Deviation

    Knockout of LMO4 in UB/OC1 cells. A, Green fluorescent cells indicate the presence of GFP in UB/OC1 cells transfected with CRISPR/Cas9 knockout plasmid. GFP is not detected in the wild-type cells. The images are representative of three biological replicates. Scale bar represents 200 μm. B, Immunoblots indicate that LMO4 protein is not detected in the knockout cells. Actin is used for normalization. The images are representative of three biological replicates. C, Modified DNA sequence of target locus in the knockout cells indicates a 6 base pair deletion.

    Journal: Journal of cellular biochemistry

    Article Title: CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth

    doi: 10.1002/jcb.26529

    Figure Lengend Snippet: Knockout of LMO4 in UB/OC1 cells. A, Green fluorescent cells indicate the presence of GFP in UB/OC1 cells transfected with CRISPR/Cas9 knockout plasmid. GFP is not detected in the wild-type cells. The images are representative of three biological replicates. Scale bar represents 200 μm. B, Immunoblots indicate that LMO4 protein is not detected in the knockout cells. Actin is used for normalization. The images are representative of three biological replicates. C, Modified DNA sequence of target locus in the knockout cells indicates a 6 base pair deletion.

    Article Snippet: The separated proteins were transferred on to apolyvinylidene difluoride membrane, blocked with 5% fat-free milk in tris-buffered saline containing 0.05% Tween 20, and incubated overnight with rabbit anti-LMO4 (1:1000, ab39383, Abcam, Cambridge, MA).

    Techniques: Knock-Out, Transfection, CRISPR, Plasmid Preparation, Western Blot, Modification, Sequencing

    Migratory potential of LMO4 knockout UB/OC1 cells. Wound healing assay suggests that the migratory potential of LMO4 knockout cells is relatively slower than that of the control cells. The wound closure in control cells after 24 h is considered as 100%. The results are expressed as mean ± standard deviation, n = 3. Significant differences between the groups were determined by one-tailed t -tests, * indicates P

    Journal: Journal of cellular biochemistry

    Article Title: CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth

    doi: 10.1002/jcb.26529

    Figure Lengend Snippet: Migratory potential of LMO4 knockout UB/OC1 cells. Wound healing assay suggests that the migratory potential of LMO4 knockout cells is relatively slower than that of the control cells. The wound closure in control cells after 24 h is considered as 100%. The results are expressed as mean ± standard deviation, n = 3. Significant differences between the groups were determined by one-tailed t -tests, * indicates P

    Article Snippet: The separated proteins were transferred on to apolyvinylidene difluoride membrane, blocked with 5% fat-free milk in tris-buffered saline containing 0.05% Tween 20, and incubated overnight with rabbit anti-LMO4 (1:1000, ab39383, Abcam, Cambridge, MA).

    Techniques: Knock-Out, Wound Healing Assay, Standard Deviation, One-tailed Test

    Differentiation of LMO4 knockout UB/OC1 cells. Myosin Vila, a biomarker of hair cells, is detected in both the wild-type and knockout cells suggesting that the knockout of LMO4 does not alter the differentiation of UB/OC1 cells. Red staining indicates immunoreactivity to antimyosin Vila, green indicates actin staining with phalloidin, while blue indicates nuclear staining with DAPI. The images are representative of three biological replicates. Scale bar represents 20 μm.

    Journal: Journal of cellular biochemistry

    Article Title: CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth

    doi: 10.1002/jcb.26529

    Figure Lengend Snippet: Differentiation of LMO4 knockout UB/OC1 cells. Myosin Vila, a biomarker of hair cells, is detected in both the wild-type and knockout cells suggesting that the knockout of LMO4 does not alter the differentiation of UB/OC1 cells. Red staining indicates immunoreactivity to antimyosin Vila, green indicates actin staining with phalloidin, while blue indicates nuclear staining with DAPI. The images are representative of three biological replicates. Scale bar represents 20 μm.

    Article Snippet: The separated proteins were transferred on to apolyvinylidene difluoride membrane, blocked with 5% fat-free milk in tris-buffered saline containing 0.05% Tween 20, and incubated overnight with rabbit anti-LMO4 (1:1000, ab39383, Abcam, Cambridge, MA).

    Techniques: Knock-Out, Biomarker Assay, Staining

    Viability of LMO4 knockout cells after cisplatin treatment. Cell counts indicate that cisplatin treatment decreases the viability of UB/OC1 cells. However, the number of viable cells was much lower in the LMO4 knockout cells relative to the control and wild-type cells. The results are expressed as mean ± standard deviation, n = 3. Significant differences between the groups were determined by one-tailed i-tests, * indicates P

    Journal: Journal of cellular biochemistry

    Article Title: CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth

    doi: 10.1002/jcb.26529

    Figure Lengend Snippet: Viability of LMO4 knockout cells after cisplatin treatment. Cell counts indicate that cisplatin treatment decreases the viability of UB/OC1 cells. However, the number of viable cells was much lower in the LMO4 knockout cells relative to the control and wild-type cells. The results are expressed as mean ± standard deviation, n = 3. Significant differences between the groups were determined by one-tailed i-tests, * indicates P

    Article Snippet: The separated proteins were transferred on to apolyvinylidene difluoride membrane, blocked with 5% fat-free milk in tris-buffered saline containing 0.05% Tween 20, and incubated overnight with rabbit anti-LMO4 (1:1000, ab39383, Abcam, Cambridge, MA).

    Techniques: Knock-Out, Standard Deviation, One-tailed Test

    Schematic of the experimental design for generating LMO4 knockout UB/OC1 cells. UB/OC1 cells were transfected with LMO4 CRISPR/Cas9 knockout plasmids containing target-specific guide RNAs and GFP. Successful transfection of the plasmid was verified by the expression of GFP. The transfected cells containing GFP were isolated by cell sorting and propagated for further analysis.

    Journal: Journal of cellular biochemistry

    Article Title: CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth

    doi: 10.1002/jcb.26529

    Figure Lengend Snippet: Schematic of the experimental design for generating LMO4 knockout UB/OC1 cells. UB/OC1 cells were transfected with LMO4 CRISPR/Cas9 knockout plasmids containing target-specific guide RNAs and GFP. Successful transfection of the plasmid was verified by the expression of GFP. The transfected cells containing GFP were isolated by cell sorting and propagated for further analysis.

    Article Snippet: The separated proteins were transferred on to apolyvinylidene difluoride membrane, blocked with 5% fat-free milk in tris-buffered saline containing 0.05% Tween 20, and incubated overnight with rabbit anti-LMO4 (1:1000, ab39383, Abcam, Cambridge, MA).

    Techniques: Knock-Out, Transfection, CRISPR, Plasmid Preparation, Expressing, Isolation, FACS