caspase 3 assay kit  (Thermo Fisher)


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    EnzChek Caspase 3 Assay Kit 2
    Description:
    The EnzChek Caspase 3 Assay Kit 2 enables detection of apoptosis by providing a simple and reliable method for assaying caspase 3 7 activity The kit can be used to continuously measure the activity of caspase 3 7 in cell extracts and purified enzyme preparations using a fluorometer or fluorescence microplate reader See our complete line of Fluorescence Microplate assays • Fluorescent assay using standard fluorescein FITC excitation emission settings • Format allows for continuous measurement of caspase 3 7 activity in cell extracts • Fluorescent and enzymatic controls included The rhodamine 110 derived substrate Z DEVD R110 used in this assay is a non fluorescent bisamide compound that upon enzymatic cleavage is converted in a two step process to the fluorescent monoamide and then to the even more fluorescent R110 product Both of these hydrolysis products exhibit spectral properties similar to those of fluorescein with peak excitation and emission wavelengths of 496 nm and 520 nm respectively In addition to the Z DEVD R110 substrate the EnzChek Caspase Assay Kit 2 contains the reversible aldehyde inhibitor Ac DEVD CHO as well as the reference standard R110 The Ac DEVD CHO inhibitor confirms that the fluorescence signals in both induced and control cell populations are due to the activity of caspase 3 7 The reference standard allows for quantification of the amount of R110 released in the reaction
    Catalog Number:
    e13184
    Price:
    None
    Applications:
    Apoptosis|Apoptosis Related Factors|Caspase Activation|Cell Analysis|Enzyme & Protein Activity Assays|Protease⁄Peptidase Activity|Protein Assays and Analysis|Protein Biology|Cell Viability, Proliferation & Function
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher caspase 3 assay kit
    MIOX gene disruption inhibits, whereas its overexpression accentuates, renal tubular cell apoptosis. Expression of proapoptogenic Bax was increased after cisplatin treatment in kidney tubules of WT mice (D versus A, and M). It was highly accentuated in MIOX-TG mice (E versus B, and M). No increase was observed in MIOX-KO mice (F versus C, and M). Intriguingly, at times a mild decrease in BAX expression was noted in KO mice (M), whereas antiapoptogenic Bcl-2 expression drastically decreased in both the WT and MIOX-TG mice, and it was unchanged in KO mice (M). An increased expression of cleaved <t>caspase-3</t> was observed in kidneys of WT mice after cisplatin treatment (M), which was accentuated in MIOX-TG mice. No expression of cleaved caspase-3 was observed in treated or untreated MIOX-KO mice (M). After cisplatin treatment, a marked increase in caspase-3 activity was observed in MIOX-TG mice, whereas a moderate increase was also observed in WT mice (N). No significant increase in caspase-3 was observed in kidneys of KO mice. Along these lines, a fulminant degree of apoptosis was observed in kidneys of MIOX-TG mice (K versus H), although a mild-to-moderate degree of apoptosis was also observed in WT mice (J versus G), and no apoptosis was detected in MIOX-KO mice (I and L).
    The EnzChek Caspase 3 Assay Kit 2 enables detection of apoptosis by providing a simple and reliable method for assaying caspase 3 7 activity The kit can be used to continuously measure the activity of caspase 3 7 in cell extracts and purified enzyme preparations using a fluorometer or fluorescence microplate reader See our complete line of Fluorescence Microplate assays • Fluorescent assay using standard fluorescein FITC excitation emission settings • Format allows for continuous measurement of caspase 3 7 activity in cell extracts • Fluorescent and enzymatic controls included The rhodamine 110 derived substrate Z DEVD R110 used in this assay is a non fluorescent bisamide compound that upon enzymatic cleavage is converted in a two step process to the fluorescent monoamide and then to the even more fluorescent R110 product Both of these hydrolysis products exhibit spectral properties similar to those of fluorescein with peak excitation and emission wavelengths of 496 nm and 520 nm respectively In addition to the Z DEVD R110 substrate the EnzChek Caspase Assay Kit 2 contains the reversible aldehyde inhibitor Ac DEVD CHO as well as the reference standard R110 The Ac DEVD CHO inhibitor confirms that the fluorescence signals in both induced and control cell populations are due to the activity of caspase 3 7 The reference standard allows for quantification of the amount of R110 released in the reaction
    https://www.bioz.com/result/caspase 3 assay kit/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    caspase 3 assay kit - by Bioz Stars, 2021-01
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    bafa1
    fitc-labeled mouse-secondary antibody
    anti-mouse - secondary
    anti-rabbit secondary antibody
    phosphate buffer saline
    caspase-3 activity assay caspase-3 activity

    Images

    1) Product Images from "Beneficial Effects of Myo-Inositol Oxygenase Deficiency in Cisplatin-Induced AKI"

    Article Title: Beneficial Effects of Myo-Inositol Oxygenase Deficiency in Cisplatin-Induced AKI

    Journal: Journal of the American Society of Nephrology : JASN

    doi: 10.1681/ASN.2016070744

    MIOX gene disruption inhibits, whereas its overexpression accentuates, renal tubular cell apoptosis. Expression of proapoptogenic Bax was increased after cisplatin treatment in kidney tubules of WT mice (D versus A, and M). It was highly accentuated in MIOX-TG mice (E versus B, and M). No increase was observed in MIOX-KO mice (F versus C, and M). Intriguingly, at times a mild decrease in BAX expression was noted in KO mice (M), whereas antiapoptogenic Bcl-2 expression drastically decreased in both the WT and MIOX-TG mice, and it was unchanged in KO mice (M). An increased expression of cleaved caspase-3 was observed in kidneys of WT mice after cisplatin treatment (M), which was accentuated in MIOX-TG mice. No expression of cleaved caspase-3 was observed in treated or untreated MIOX-KO mice (M). After cisplatin treatment, a marked increase in caspase-3 activity was observed in MIOX-TG mice, whereas a moderate increase was also observed in WT mice (N). No significant increase in caspase-3 was observed in kidneys of KO mice. Along these lines, a fulminant degree of apoptosis was observed in kidneys of MIOX-TG mice (K versus H), although a mild-to-moderate degree of apoptosis was also observed in WT mice (J versus G), and no apoptosis was detected in MIOX-KO mice (I and L).
    Figure Legend Snippet: MIOX gene disruption inhibits, whereas its overexpression accentuates, renal tubular cell apoptosis. Expression of proapoptogenic Bax was increased after cisplatin treatment in kidney tubules of WT mice (D versus A, and M). It was highly accentuated in MIOX-TG mice (E versus B, and M). No increase was observed in MIOX-KO mice (F versus C, and M). Intriguingly, at times a mild decrease in BAX expression was noted in KO mice (M), whereas antiapoptogenic Bcl-2 expression drastically decreased in both the WT and MIOX-TG mice, and it was unchanged in KO mice (M). An increased expression of cleaved caspase-3 was observed in kidneys of WT mice after cisplatin treatment (M), which was accentuated in MIOX-TG mice. No expression of cleaved caspase-3 was observed in treated or untreated MIOX-KO mice (M). After cisplatin treatment, a marked increase in caspase-3 activity was observed in MIOX-TG mice, whereas a moderate increase was also observed in WT mice (N). No significant increase in caspase-3 was observed in kidneys of KO mice. Along these lines, a fulminant degree of apoptosis was observed in kidneys of MIOX-TG mice (K versus H), although a mild-to-moderate degree of apoptosis was also observed in WT mice (J versus G), and no apoptosis was detected in MIOX-KO mice (I and L).

    Techniques Used: Over Expression, Expressing, Mouse Assay, Activity Assay

    2) Product Images from "ROCK inhibitor reduces Myc-induced apoptosis and mediates immortalization of human keratinocytes"

    Article Title: ROCK inhibitor reduces Myc-induced apoptosis and mediates immortalization of human keratinocytes

    Journal: Oncotarget

    doi: 10.18632/oncotarget.11458

    Myc increases apoptosis and detachment of cultured cells ( A and B ). Myc-transduced keratinocytes exhibit higher levels of apoptosis as measured by active caspase-3 in attached cells. ( C ) Myc transduced keratinocyte cultures exhibit more detached cells. ( D ). A majority of floaters are apoptotic.
    Figure Legend Snippet: Myc increases apoptosis and detachment of cultured cells ( A and B ). Myc-transduced keratinocytes exhibit higher levels of apoptosis as measured by active caspase-3 in attached cells. ( C ) Myc transduced keratinocyte cultures exhibit more detached cells. ( D ). A majority of floaters are apoptotic.

    Techniques Used: Cell Culture

    Y-27632 inhibits apoptosis of Myc-expressing cells ( A and B ) Y-27632 reduces caspase-3 activity in Myc-expressing HFK (attached cells). The level of caspase-3 activity in attached Myc cells was evaluated by immunofluorescence using Ab against active caspase-3 (Cell Signaling). Cells were stained and positive caspase 3 cells were counted. Apoptosis was significantly inhibited from control in the presence of Y-27632 (* P
    Figure Legend Snippet: Y-27632 inhibits apoptosis of Myc-expressing cells ( A and B ) Y-27632 reduces caspase-3 activity in Myc-expressing HFK (attached cells). The level of caspase-3 activity in attached Myc cells was evaluated by immunofluorescence using Ab against active caspase-3 (Cell Signaling). Cells were stained and positive caspase 3 cells were counted. Apoptosis was significantly inhibited from control in the presence of Y-27632 (* P

    Techniques Used: Expressing, Activity Assay, Immunofluorescence, Staining

    Y-27632 suppresses caspase-3 in response to inducers of extrinsic or intrinsic apoptosis Primary keratinocytes were established and unexposed or exposed to Y-27632 for 24 h, then treated with α-Fas agonist Ab ( A ), TNFα ( B ), etoposide ( C ), or camptothecin ( D ) for an additional 24 h. Cytosolic extracts were derived and subjected to fluorometric caspase-3 activity assays using fluorescent tetrapeptide substrate specific for caspases-3 as described in Materials and Methods. Free AMC, generated as a result of cleavage of the aspartate-AMC bond, was monitored over 30 min. Emission at 460nm from each sample was plotted against time, and linear regression analysis was used to determine the initial velocity (slope) for each curve, which yielded the activity ( E ). Inhibitor of caspase-3 (Z-DEVD-CHO) (BioMol, Plymouth Meeting, PA) was added to cell extracts at a concentration of 50 μM, 10 min prior to addition of the Ac-DEVD-AMC as a control.
    Figure Legend Snippet: Y-27632 suppresses caspase-3 in response to inducers of extrinsic or intrinsic apoptosis Primary keratinocytes were established and unexposed or exposed to Y-27632 for 24 h, then treated with α-Fas agonist Ab ( A ), TNFα ( B ), etoposide ( C ), or camptothecin ( D ) for an additional 24 h. Cytosolic extracts were derived and subjected to fluorometric caspase-3 activity assays using fluorescent tetrapeptide substrate specific for caspases-3 as described in Materials and Methods. Free AMC, generated as a result of cleavage of the aspartate-AMC bond, was monitored over 30 min. Emission at 460nm from each sample was plotted against time, and linear regression analysis was used to determine the initial velocity (slope) for each curve, which yielded the activity ( E ). Inhibitor of caspase-3 (Z-DEVD-CHO) (BioMol, Plymouth Meeting, PA) was added to cell extracts at a concentration of 50 μM, 10 min prior to addition of the Ac-DEVD-AMC as a control.

    Techniques Used: Derivative Assay, Activity Assay, Generated, Concentration Assay

    3) Product Images from "Essential oil of Curcuma wenyujin induces apoptosis in human hepatoma cells"

    Article Title: Essential oil of Curcuma wenyujin induces apoptosis in human hepatoma cells

    Journal: World Journal of Gastroenterology : WJG

    doi: 10.3748/wjg.14.4309

    Protein expression levels of active caspase-3 and cleaved PARP in CWO-induced apoptosis. HepG2 cells were treated with medium alone (control) or different concentration of CWO for 48 h. Cells of each sample were counted to 1.0 × 10 6 and all the
    Figure Legend Snippet: Protein expression levels of active caspase-3 and cleaved PARP in CWO-induced apoptosis. HepG2 cells were treated with medium alone (control) or different concentration of CWO for 48 h. Cells of each sample were counted to 1.0 × 10 6 and all the

    Techniques Used: Expressing, Concentration Assay

    The fluorescence was increased in a dose-dependant manner after 24 h treatment with CWO. Caspase-3 cleaves substrate Ac-DEVD-R110 to emit green fluorescence. Higher fluorescent intensity indicates higher caspase-3 enzymatic activity. Ac-DEVE-CHO inhibitor
    Figure Legend Snippet: The fluorescence was increased in a dose-dependant manner after 24 h treatment with CWO. Caspase-3 cleaves substrate Ac-DEVD-R110 to emit green fluorescence. Higher fluorescent intensity indicates higher caspase-3 enzymatic activity. Ac-DEVE-CHO inhibitor

    Techniques Used: Fluorescence, Activity Assay

    4) Product Images from "In vitro effect of progesterone on human melanoma (BLM) cell growth"

    Article Title: In vitro effect of progesterone on human melanoma (BLM) cell growth

    Journal: International Journal of Clinical and Experimental Medicine

    doi:

    Mechanism of cell death-Apoptosis. In order to check whether the mechanism of cell death was due to apoptosis, cells after incubation with progesterone for 48 hrs. were subjected to DAPI staining, DNA analysis by agarose gel electrophoresis, and caspase-3
    Figure Legend Snippet: Mechanism of cell death-Apoptosis. In order to check whether the mechanism of cell death was due to apoptosis, cells after incubation with progesterone for 48 hrs. were subjected to DAPI staining, DNA analysis by agarose gel electrophoresis, and caspase-3

    Techniques Used: Incubation, Staining, Agarose Gel Electrophoresis

    5) Product Images from "Remodeling of the tight junction during recovery from exposure to hydrogen peroxide in kidney epithelial cells"

    Article Title: Remodeling of the tight junction during recovery from exposure to hydrogen peroxide in kidney epithelial cells

    Journal: Free radical biology & medicine

    doi: 10.1016/j.freeradbiomed.2009.08.024

    ERK inhibition potentiates caspase-3 activity during recovery from H 2 O 2 exposure. Cells were grown in 24-well culture plates and exposed to 3mM H 2 O 2 for 60 minutes, then recovered for up to 6 hours. U0126 (10μM) was added 30 minutes prior to H
    Figure Legend Snippet: ERK inhibition potentiates caspase-3 activity during recovery from H 2 O 2 exposure. Cells were grown in 24-well culture plates and exposed to 3mM H 2 O 2 for 60 minutes, then recovered for up to 6 hours. U0126 (10μM) was added 30 minutes prior to H

    Techniques Used: Inhibition, Activity Assay

    6) Product Images from "FADD regulates NF-κB activation and promotes ubiquitination of cFLIPL to induce apoptosis"

    Article Title: FADD regulates NF-κB activation and promotes ubiquitination of cFLIPL to induce apoptosis

    Journal: Scientific Reports

    doi: 10.1038/srep22787

    FADD augments downstream apoptosis signaling in TNF-α stimulated cells. TNF-α (10 ng/ml) was subjected to HEK 293T cells and 48 h of pcDNA-FADD transfected HEK 293T cells. Control represents HEK 293T cells without TNF-α treatment (black bar) and 48 h pcDNA-FADD transfcted HEK 293T cells (white bar) (shown as 0 h time point). ( a ) Percent cell viability, ( b ) Percent cell proliferation, ( c ) Images of Propidium iodide (PI) staining (pEYFP-FADD construct was used in this experiment) (scale bar-2 μm), ( d ) Apoptotic cell death monitored by Flow cytometric analysis using (BD FACSAria 3, BD Biosciences, San Jose, CA, USA BD), the result represents in contour plots with quadrant gates showing early apoptotic shown in quadrant 4 (Q4) and late apoptotic in quadrant 2 (Q2), ( e ) Percent apoptotic death (Annexin-V-FITC + /PI + ) by Tali TM image based cytometer, ( f) Expression of of cell death regulatory proteins examined by Western blotting, (g) caspase-8 activity, ( h ) caspase-3 activity. The uncropped full-length blot of Procaspase-8, processed caspase-3 and cytochrome c are presented in supplementary Fig. S10 . Next, HEK 293T cells were treated as mentioned in Figure legend 2i, TNF-α untreated and non targeting siRNA transfected cells were taken as control. Here results illustrate ( i) Quantitative analysis of loss of mitochondrial membrane potential (Ψ), ( j ) Percent apoptotic death (Annexin-V-FITC + /PI + ) by Tali TM image based cytometer, ( k ) Caspase-3 activity, ( l ) Representative images of Western blot for cell death regulatory proteins. The uncropped full-length blot of FADD is presented in supplementary Fig. S10 . Note that, the y axis break in ( a , b ) indicates the scale has been compressed between 97–99% and in ( e ) scale has been compressed between 7–10%. Error bars represent mean ± SD; In ( a , b , e , g , h ), *P ≤ 0.05 **P ≤ 0.001, TNF-α treatment in non-transfected vs FADD transfected cells (One way ANOVA followed by Student Newman-Keuls test, n = 4), In ( i – k ), *P ≤ 0.05, control vs TNF-α or cFLIP L KD or TNF-α + cFLIP L KD , (student t-test, n = 4), where n is the number of independent experiments.
    Figure Legend Snippet: FADD augments downstream apoptosis signaling in TNF-α stimulated cells. TNF-α (10 ng/ml) was subjected to HEK 293T cells and 48 h of pcDNA-FADD transfected HEK 293T cells. Control represents HEK 293T cells without TNF-α treatment (black bar) and 48 h pcDNA-FADD transfcted HEK 293T cells (white bar) (shown as 0 h time point). ( a ) Percent cell viability, ( b ) Percent cell proliferation, ( c ) Images of Propidium iodide (PI) staining (pEYFP-FADD construct was used in this experiment) (scale bar-2 μm), ( d ) Apoptotic cell death monitored by Flow cytometric analysis using (BD FACSAria 3, BD Biosciences, San Jose, CA, USA BD), the result represents in contour plots with quadrant gates showing early apoptotic shown in quadrant 4 (Q4) and late apoptotic in quadrant 2 (Q2), ( e ) Percent apoptotic death (Annexin-V-FITC + /PI + ) by Tali TM image based cytometer, ( f) Expression of of cell death regulatory proteins examined by Western blotting, (g) caspase-8 activity, ( h ) caspase-3 activity. The uncropped full-length blot of Procaspase-8, processed caspase-3 and cytochrome c are presented in supplementary Fig. S10 . Next, HEK 293T cells were treated as mentioned in Figure legend 2i, TNF-α untreated and non targeting siRNA transfected cells were taken as control. Here results illustrate ( i) Quantitative analysis of loss of mitochondrial membrane potential (Ψ), ( j ) Percent apoptotic death (Annexin-V-FITC + /PI + ) by Tali TM image based cytometer, ( k ) Caspase-3 activity, ( l ) Representative images of Western blot for cell death regulatory proteins. The uncropped full-length blot of FADD is presented in supplementary Fig. S10 . Note that, the y axis break in ( a , b ) indicates the scale has been compressed between 97–99% and in ( e ) scale has been compressed between 7–10%. Error bars represent mean ± SD; In ( a , b , e , g , h ), *P ≤ 0.05 **P ≤ 0.001, TNF-α treatment in non-transfected vs FADD transfected cells (One way ANOVA followed by Student Newman-Keuls test, n = 4), In ( i – k ), *P ≤ 0.05, control vs TNF-α or cFLIP L KD or TNF-α + cFLIP L KD , (student t-test, n = 4), where n is the number of independent experiments.

    Techniques Used: Transfection, Staining, Construct, Flow Cytometry, Cytometry, Expressing, Western Blot, Activity Assay

    7) Product Images from "Involvement of Dynamin-Related Protein 1 in Free Fatty Acid-Induced INS-1-Derived Cell Apoptosis"

    Article Title: Involvement of Dynamin-Related Protein 1 in Free Fatty Acid-Induced INS-1-Derived Cell Apoptosis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0049258

    The effect of DRP-1 on ROS production and caspase-3 activity in DRP-1 WT and DRP-1 K38A cells. Data are presented as mean ± S.E. of three different experiments conducted in triplicate (A, B) The effect of DRP-1 on ROS production in DRP-1 WT and DRP-1 K38A cells (* p
    Figure Legend Snippet: The effect of DRP-1 on ROS production and caspase-3 activity in DRP-1 WT and DRP-1 K38A cells. Data are presented as mean ± S.E. of three different experiments conducted in triplicate (A, B) The effect of DRP-1 on ROS production in DRP-1 WT and DRP-1 K38A cells (* p

    Techniques Used: Activity Assay

    8) Product Images from "Recognition of G-quadruplex topology through hybrid binding with implications in cancer theranostics"

    Article Title: Recognition of G-quadruplex topology through hybrid binding with implications in cancer theranostics

    Journal: Theranostics

    doi: 10.7150/thno.48675

    In vitro antiproliferative effect of TGP18 through apoptotic pathway. ( A ) Antiproliferative effect of TGP18 on different cancer (HeLa, MDA-MB-231, A549, and MCF-7) and non-tumorogenic (HEK293T) cell lines treated for 24 h. ( B ) Cell cycle histogram of A549 cells after 24 h treatment with TGP18 at 2, 5 and 10 μM. Cells were analyzed by fluorescence-activated cell sorting (FACS) with the intensity of DAPI recorded. ( C ) The representative FACS profile shows the mean percentage of the cell population in G0/G1, S and G2/M phase (with 95% CIs) under different concentrations (1, 2, 5 and 10 μM) of TGP18; confidence intervals (CIs). ( D ) Effect of TGP18 (5 and 10 μM) and camptothecin (10 μM) treatment on caspase-3 activity in A549 cells for 24 h. ( E ) Transcription expression profile of C-MYC, KRAS, and BCL-2 upon treatment of TGP18 with increasing concentrations. Quantification of mRNA expression level relative to control (GAPDH) by RT-PCR in A549 cells. Error bars represent mean ± SE (N = 3). Statistical differences are determined compared to the control by two-tailed student's t -test (* P
    Figure Legend Snippet: In vitro antiproliferative effect of TGP18 through apoptotic pathway. ( A ) Antiproliferative effect of TGP18 on different cancer (HeLa, MDA-MB-231, A549, and MCF-7) and non-tumorogenic (HEK293T) cell lines treated for 24 h. ( B ) Cell cycle histogram of A549 cells after 24 h treatment with TGP18 at 2, 5 and 10 μM. Cells were analyzed by fluorescence-activated cell sorting (FACS) with the intensity of DAPI recorded. ( C ) The representative FACS profile shows the mean percentage of the cell population in G0/G1, S and G2/M phase (with 95% CIs) under different concentrations (1, 2, 5 and 10 μM) of TGP18; confidence intervals (CIs). ( D ) Effect of TGP18 (5 and 10 μM) and camptothecin (10 μM) treatment on caspase-3 activity in A549 cells for 24 h. ( E ) Transcription expression profile of C-MYC, KRAS, and BCL-2 upon treatment of TGP18 with increasing concentrations. Quantification of mRNA expression level relative to control (GAPDH) by RT-PCR in A549 cells. Error bars represent mean ± SE (N = 3). Statistical differences are determined compared to the control by two-tailed student's t -test (* P

    Techniques Used: In Vitro, Multiple Displacement Amplification, Fluorescence, FACS, Activity Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Two Tailed Test

    9) Product Images from "TRB3 Is Involved in Free Fatty Acid-Induced INS-1-Derived Cell Apoptosis via the Protein Kinase C ? Pathway"

    Article Title: TRB3 Is Involved in Free Fatty Acid-Induced INS-1-Derived Cell Apoptosis via the Protein Kinase C ? Pathway

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0096089

    The effect of TRB3 knockdown on palmitate-induced apoptosis. Western blot was used to confirm TRB3 silencing efficiency in INA-1 cells (A). (B and C) TRB3 knockdown inhibited palmitate-induced apoptosis in INS-1 cells. After transfection with TRB3 siRNA for 2 days, INS-1 cells were treated with or without 0.2 mM palmitate for 24 hours. Cell apoptosis was assessed by TUNEL staining (B) and caspase-3 activity assay (C). Columns represent mean ± S.E.M. of three independent experiments conducted in triplicate (* p
    Figure Legend Snippet: The effect of TRB3 knockdown on palmitate-induced apoptosis. Western blot was used to confirm TRB3 silencing efficiency in INA-1 cells (A). (B and C) TRB3 knockdown inhibited palmitate-induced apoptosis in INS-1 cells. After transfection with TRB3 siRNA for 2 days, INS-1 cells were treated with or without 0.2 mM palmitate for 24 hours. Cell apoptosis was assessed by TUNEL staining (B) and caspase-3 activity assay (C). Columns represent mean ± S.E.M. of three independent experiments conducted in triplicate (* p

    Techniques Used: Western Blot, Transfection, TUNEL Assay, Staining, Caspase-3 Activity Assay

    10) Product Images from "Resveratrol-loaded PLGA nanoparticles mediated programmed cell death in prostate cancer cells"

    Article Title: Resveratrol-loaded PLGA nanoparticles mediated programmed cell death in prostate cancer cells

    Journal: Saudi Pharmaceutical Journal : SPJ

    doi: 10.1016/j.jsps.2018.03.009

    Effects of different concentrations of RL and RLPLGA nanoparticles on caspase-3 activity within LNCaP cells.
    Figure Legend Snippet: Effects of different concentrations of RL and RLPLGA nanoparticles on caspase-3 activity within LNCaP cells.

    Techniques Used: Activity Assay

    11) Product Images from "The Alleviative Effect of Vitamin B2 on Potassium Bromate-Induced Hepatotoxicity in Male Rats"

    Article Title: The Alleviative Effect of Vitamin B2 on Potassium Bromate-Induced Hepatotoxicity in Male Rats

    Journal: BioMed Research International

    doi: 10.1155/2020/8274261

    Bar graphs showing the activity of caspase-3 and lactate dehydrogenase (LDH) in the liver samples of indicated animal groups. All data has been expressed in the mean ± SD of six independent experiments. The marks “a, b, and c” were used as asterisk marks to show significance difference from the negative control (CN − , group I) at p less than 0.05, 0.005, and 0.001 while the marks “x, y, and z” were used as asterisk marks to show significance difference from the positive control (CN + , group II) at p less than 0.05, 0.005, and 0.001.
    Figure Legend Snippet: Bar graphs showing the activity of caspase-3 and lactate dehydrogenase (LDH) in the liver samples of indicated animal groups. All data has been expressed in the mean ± SD of six independent experiments. The marks “a, b, and c” were used as asterisk marks to show significance difference from the negative control (CN − , group I) at p less than 0.05, 0.005, and 0.001 while the marks “x, y, and z” were used as asterisk marks to show significance difference from the positive control (CN + , group II) at p less than 0.05, 0.005, and 0.001.

    Techniques Used: Activity Assay, Negative Control, Positive Control

    12) Product Images from "Essential oil of Curcuma wenyujin induces apoptosis in human hepatoma cells"

    Article Title: Essential oil of Curcuma wenyujin induces apoptosis in human hepatoma cells

    Journal: World Journal of Gastroenterology : WJG

    doi: 10.3748/wjg.14.4309

    Protein expression levels of active caspase-3 and cleaved PARP in CWO-induced apoptosis. HepG2 cells were treated with medium alone (control) or different concentration of CWO for 48 h. Cells of each sample were counted to 1.0 × 10 6 and all the
    Figure Legend Snippet: Protein expression levels of active caspase-3 and cleaved PARP in CWO-induced apoptosis. HepG2 cells were treated with medium alone (control) or different concentration of CWO for 48 h. Cells of each sample were counted to 1.0 × 10 6 and all the

    Techniques Used: Expressing, Concentration Assay

    The fluorescence was increased in a dose-dependant manner after 24 h treatment with CWO. Caspase-3 cleaves substrate Ac-DEVD-R110 to emit green fluorescence. Higher fluorescent intensity indicates higher caspase-3 enzymatic activity. Ac-DEVE-CHO inhibitor
    Figure Legend Snippet: The fluorescence was increased in a dose-dependant manner after 24 h treatment with CWO. Caspase-3 cleaves substrate Ac-DEVD-R110 to emit green fluorescence. Higher fluorescent intensity indicates higher caspase-3 enzymatic activity. Ac-DEVE-CHO inhibitor

    Techniques Used: Fluorescence, Activity Assay

    Related Articles

    TUNEL Assay:

    Article Title: Isoflavone genistein protects human vascular endothelial cells against tumor necrosis factor-?-induced apoptosis through the p38? mitogen-activated protein kinase
    Article Snippet: .. Primary HAECs, fetal bovine serum (FBS) and endothelial growth supplements (EGM2) were purchased from Lonza Walkersville ( Walkersville, MD); M199 media, caspase-3 assay kits and competent cells for plasmid transformation were from Invitrogen (Carlsbad, CA); antibodies against p38, phospho-p38 (Thrl80/Tyrl82), p38α, p38β, Bcl-2 and β-actin were from Cell Signaling Technology (Beverly, MA); supersignal chemiluminescence detection system and Protein A beads were from Pierce (Rockford, IL); nitrocellulose membranes and protein assay kits were from Bio-Rad (Hercules, CA); Bcl-2 promoter-driven luciferase (Bcl-2-Luc) reporter construct was a kind gift from Dr. Linda M. Boxer, Stanford University, Stanford, CA); plasmid purification kit was from Qiagen (Valencia, CA); transfection reagents were from Targeting System (Santee, CA); dual luciferase reporter assay kits were from Promega (Madison, WI); terminal deoxynucleotidyltransferase dUTP nick-end labeling (TUNEL) and apoptotic DNA ladder kits were from Roche Applied Science (Indianapolis, IN); ICI182,780 was from Tocris (St. Louis, MO); genistein, TNF-α, SB203580, SB203474, H89, PD098059, protease and phosphatase inhibitor cocktails, 7-amino actinomycin D (7-AAD) and other general chemicals were from Sigma (St. Louis, MO); stock solutions of genistein, at 20 mM in dimethylsulfoxide (DMSO), were stored at −80°C before use. .. Primary HAECs were cultured in M199 medium containing 2% FBS and endothelial growth supplements-EGM2 at 37°C in a 5% CO2 /95% air environment.

    Transfection:

    Article Title: Isoflavone genistein protects human vascular endothelial cells against tumor necrosis factor-?-induced apoptosis through the p38? mitogen-activated protein kinase
    Article Snippet: .. Primary HAECs, fetal bovine serum (FBS) and endothelial growth supplements (EGM2) were purchased from Lonza Walkersville ( Walkersville, MD); M199 media, caspase-3 assay kits and competent cells for plasmid transformation were from Invitrogen (Carlsbad, CA); antibodies against p38, phospho-p38 (Thrl80/Tyrl82), p38α, p38β, Bcl-2 and β-actin were from Cell Signaling Technology (Beverly, MA); supersignal chemiluminescence detection system and Protein A beads were from Pierce (Rockford, IL); nitrocellulose membranes and protein assay kits were from Bio-Rad (Hercules, CA); Bcl-2 promoter-driven luciferase (Bcl-2-Luc) reporter construct was a kind gift from Dr. Linda M. Boxer, Stanford University, Stanford, CA); plasmid purification kit was from Qiagen (Valencia, CA); transfection reagents were from Targeting System (Santee, CA); dual luciferase reporter assay kits were from Promega (Madison, WI); terminal deoxynucleotidyltransferase dUTP nick-end labeling (TUNEL) and apoptotic DNA ladder kits were from Roche Applied Science (Indianapolis, IN); ICI182,780 was from Tocris (St. Louis, MO); genistein, TNF-α, SB203580, SB203474, H89, PD098059, protease and phosphatase inhibitor cocktails, 7-amino actinomycin D (7-AAD) and other general chemicals were from Sigma (St. Louis, MO); stock solutions of genistein, at 20 mM in dimethylsulfoxide (DMSO), were stored at −80°C before use. .. Primary HAECs were cultured in M199 medium containing 2% FBS and endothelial growth supplements-EGM2 at 37°C in a 5% CO2 /95% air environment.

    Luciferase:

    Article Title: Isoflavone genistein protects human vascular endothelial cells against tumor necrosis factor-?-induced apoptosis through the p38? mitogen-activated protein kinase
    Article Snippet: .. Primary HAECs, fetal bovine serum (FBS) and endothelial growth supplements (EGM2) were purchased from Lonza Walkersville ( Walkersville, MD); M199 media, caspase-3 assay kits and competent cells for plasmid transformation were from Invitrogen (Carlsbad, CA); antibodies against p38, phospho-p38 (Thrl80/Tyrl82), p38α, p38β, Bcl-2 and β-actin were from Cell Signaling Technology (Beverly, MA); supersignal chemiluminescence detection system and Protein A beads were from Pierce (Rockford, IL); nitrocellulose membranes and protein assay kits were from Bio-Rad (Hercules, CA); Bcl-2 promoter-driven luciferase (Bcl-2-Luc) reporter construct was a kind gift from Dr. Linda M. Boxer, Stanford University, Stanford, CA); plasmid purification kit was from Qiagen (Valencia, CA); transfection reagents were from Targeting System (Santee, CA); dual luciferase reporter assay kits were from Promega (Madison, WI); terminal deoxynucleotidyltransferase dUTP nick-end labeling (TUNEL) and apoptotic DNA ladder kits were from Roche Applied Science (Indianapolis, IN); ICI182,780 was from Tocris (St. Louis, MO); genistein, TNF-α, SB203580, SB203474, H89, PD098059, protease and phosphatase inhibitor cocktails, 7-amino actinomycin D (7-AAD) and other general chemicals were from Sigma (St. Louis, MO); stock solutions of genistein, at 20 mM in dimethylsulfoxide (DMSO), were stored at −80°C before use. .. Primary HAECs were cultured in M199 medium containing 2% FBS and endothelial growth supplements-EGM2 at 37°C in a 5% CO2 /95% air environment.

    Reporter Assay:

    Article Title: Isoflavone genistein protects human vascular endothelial cells against tumor necrosis factor-?-induced apoptosis through the p38? mitogen-activated protein kinase
    Article Snippet: .. Primary HAECs, fetal bovine serum (FBS) and endothelial growth supplements (EGM2) were purchased from Lonza Walkersville ( Walkersville, MD); M199 media, caspase-3 assay kits and competent cells for plasmid transformation were from Invitrogen (Carlsbad, CA); antibodies against p38, phospho-p38 (Thrl80/Tyrl82), p38α, p38β, Bcl-2 and β-actin were from Cell Signaling Technology (Beverly, MA); supersignal chemiluminescence detection system and Protein A beads were from Pierce (Rockford, IL); nitrocellulose membranes and protein assay kits were from Bio-Rad (Hercules, CA); Bcl-2 promoter-driven luciferase (Bcl-2-Luc) reporter construct was a kind gift from Dr. Linda M. Boxer, Stanford University, Stanford, CA); plasmid purification kit was from Qiagen (Valencia, CA); transfection reagents were from Targeting System (Santee, CA); dual luciferase reporter assay kits were from Promega (Madison, WI); terminal deoxynucleotidyltransferase dUTP nick-end labeling (TUNEL) and apoptotic DNA ladder kits were from Roche Applied Science (Indianapolis, IN); ICI182,780 was from Tocris (St. Louis, MO); genistein, TNF-α, SB203580, SB203474, H89, PD098059, protease and phosphatase inhibitor cocktails, 7-amino actinomycin D (7-AAD) and other general chemicals were from Sigma (St. Louis, MO); stock solutions of genistein, at 20 mM in dimethylsulfoxide (DMSO), were stored at −80°C before use. .. Primary HAECs were cultured in M199 medium containing 2% FBS and endothelial growth supplements-EGM2 at 37°C in a 5% CO2 /95% air environment.

    Protease Assay:

    Article Title: Antioxidant Activity of Zein Hydrolysates from Zea Species and Their Cytotoxic Effects in a Hepatic Cell Culture
    Article Snippet: .. We used 3 kits to evaluate caspase expression: caspase 3: EnzChek Caspase-3 Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA); caspase 8: ApoTarget Caspase-8 Fluorometric Protease Assay (Invitrogen, Frederick, MD, USA); and caspase 9: ApoTarget Caspase-9 Colorimetric Protease Assay (Invitrogen). ..

    Caspase-3 Assay:

    Article Title: Role of Glucuronidation for Hepatic Detoxification and Urinary Elimination of Toxic Bile Acids during Biliary Obstruction
    Article Snippet: .. The Enzchek® caspase-3 assay kit 2 was from Invitrogen (Grand Island, NY). .. Absorbance and fluorescence were quantified using a Tecan Infinite M1000 series device.

    Article Title: Pandemic Influenza A (H1N1) Virus Infection Increases Apoptosis and HIV-1 Replication in HIV-1 Infected Jurkat Cells
    Article Snippet: .. Caspase-3 Assay EnzChek® Caspase-3 Assay Kit #1, Z-DEVD-AMC Substrate from Invitrogen (Grand Island, NY, USA) was used to test caspase-3 activity, which was performed according to the manufacturer’s instruction. .. Cell Viability Assay Cell viability was determined by Trypan blue exclusion analysis (Life Technologies, Waltham, MA, USA).

    Article Title: Remodeling of the tight junction during recovery from exposure to hydrogen peroxide in kidney epithelial cells
    Article Snippet: .. The caspase-3 assay kit, polyclonal rabbit anti-occludin, anti-claudin-1, and anti-claudin-2, and Alexa 488-conjugated anti-rabbit IgG antibodies were purchased from Invitrogen (Carlsbad, CA). .. Polyclonal rabbit anti-MAP Kinase 1/2 (ERK 1/2) was purchased from Upstate Cell Signaling Solutions (Lake Placid, NY).

    Article Title: Isoflavone genistein protects human vascular endothelial cells against tumor necrosis factor-?-induced apoptosis through the p38? mitogen-activated protein kinase
    Article Snippet: .. Primary HAECs, fetal bovine serum (FBS) and endothelial growth supplements (EGM2) were purchased from Lonza Walkersville ( Walkersville, MD); M199 media, caspase-3 assay kits and competent cells for plasmid transformation were from Invitrogen (Carlsbad, CA); antibodies against p38, phospho-p38 (Thrl80/Tyrl82), p38α, p38β, Bcl-2 and β-actin were from Cell Signaling Technology (Beverly, MA); supersignal chemiluminescence detection system and Protein A beads were from Pierce (Rockford, IL); nitrocellulose membranes and protein assay kits were from Bio-Rad (Hercules, CA); Bcl-2 promoter-driven luciferase (Bcl-2-Luc) reporter construct was a kind gift from Dr. Linda M. Boxer, Stanford University, Stanford, CA); plasmid purification kit was from Qiagen (Valencia, CA); transfection reagents were from Targeting System (Santee, CA); dual luciferase reporter assay kits were from Promega (Madison, WI); terminal deoxynucleotidyltransferase dUTP nick-end labeling (TUNEL) and apoptotic DNA ladder kits were from Roche Applied Science (Indianapolis, IN); ICI182,780 was from Tocris (St. Louis, MO); genistein, TNF-α, SB203580, SB203474, H89, PD098059, protease and phosphatase inhibitor cocktails, 7-amino actinomycin D (7-AAD) and other general chemicals were from Sigma (St. Louis, MO); stock solutions of genistein, at 20 mM in dimethylsulfoxide (DMSO), were stored at −80°C before use. .. Primary HAECs were cultured in M199 medium containing 2% FBS and endothelial growth supplements-EGM2 at 37°C in a 5% CO2 /95% air environment.

    Article Title: Antioxidant Activity of Zein Hydrolysates from Zea Species and Their Cytotoxic Effects in a Hepatic Cell Culture
    Article Snippet: .. We used 3 kits to evaluate caspase expression: caspase 3: EnzChek Caspase-3 Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA); caspase 8: ApoTarget Caspase-8 Fluorometric Protease Assay (Invitrogen, Frederick, MD, USA); and caspase 9: ApoTarget Caspase-9 Colorimetric Protease Assay (Invitrogen). ..

    Article Title: Homotype-Targeted Biogenic Nanoparticles to Kill Multidrug-Resistant Cancer Cells
    Article Snippet: .. The underlying mechanism of apoptosis was studied by employing caspase-3 assay kits. .. Briefly, cells were treated with different NPs for 24 h, washed two times with PBS, and collected, followed by the addition of 200 μL of lysate buffer for the caspase-3 assay and 100 μL for the caspase-9 assay and incubation for 20 min on ice.

    Construct:

    Article Title: Isoflavone genistein protects human vascular endothelial cells against tumor necrosis factor-?-induced apoptosis through the p38? mitogen-activated protein kinase
    Article Snippet: .. Primary HAECs, fetal bovine serum (FBS) and endothelial growth supplements (EGM2) were purchased from Lonza Walkersville ( Walkersville, MD); M199 media, caspase-3 assay kits and competent cells for plasmid transformation were from Invitrogen (Carlsbad, CA); antibodies against p38, phospho-p38 (Thrl80/Tyrl82), p38α, p38β, Bcl-2 and β-actin were from Cell Signaling Technology (Beverly, MA); supersignal chemiluminescence detection system and Protein A beads were from Pierce (Rockford, IL); nitrocellulose membranes and protein assay kits were from Bio-Rad (Hercules, CA); Bcl-2 promoter-driven luciferase (Bcl-2-Luc) reporter construct was a kind gift from Dr. Linda M. Boxer, Stanford University, Stanford, CA); plasmid purification kit was from Qiagen (Valencia, CA); transfection reagents were from Targeting System (Santee, CA); dual luciferase reporter assay kits were from Promega (Madison, WI); terminal deoxynucleotidyltransferase dUTP nick-end labeling (TUNEL) and apoptotic DNA ladder kits were from Roche Applied Science (Indianapolis, IN); ICI182,780 was from Tocris (St. Louis, MO); genistein, TNF-α, SB203580, SB203474, H89, PD098059, protease and phosphatase inhibitor cocktails, 7-amino actinomycin D (7-AAD) and other general chemicals were from Sigma (St. Louis, MO); stock solutions of genistein, at 20 mM in dimethylsulfoxide (DMSO), were stored at −80°C before use. .. Primary HAECs were cultured in M199 medium containing 2% FBS and endothelial growth supplements-EGM2 at 37°C in a 5% CO2 /95% air environment.

    Purification:

    Article Title: Isoflavone genistein protects human vascular endothelial cells against tumor necrosis factor-?-induced apoptosis through the p38? mitogen-activated protein kinase
    Article Snippet: .. Primary HAECs, fetal bovine serum (FBS) and endothelial growth supplements (EGM2) were purchased from Lonza Walkersville ( Walkersville, MD); M199 media, caspase-3 assay kits and competent cells for plasmid transformation were from Invitrogen (Carlsbad, CA); antibodies against p38, phospho-p38 (Thrl80/Tyrl82), p38α, p38β, Bcl-2 and β-actin were from Cell Signaling Technology (Beverly, MA); supersignal chemiluminescence detection system and Protein A beads were from Pierce (Rockford, IL); nitrocellulose membranes and protein assay kits were from Bio-Rad (Hercules, CA); Bcl-2 promoter-driven luciferase (Bcl-2-Luc) reporter construct was a kind gift from Dr. Linda M. Boxer, Stanford University, Stanford, CA); plasmid purification kit was from Qiagen (Valencia, CA); transfection reagents were from Targeting System (Santee, CA); dual luciferase reporter assay kits were from Promega (Madison, WI); terminal deoxynucleotidyltransferase dUTP nick-end labeling (TUNEL) and apoptotic DNA ladder kits were from Roche Applied Science (Indianapolis, IN); ICI182,780 was from Tocris (St. Louis, MO); genistein, TNF-α, SB203580, SB203474, H89, PD098059, protease and phosphatase inhibitor cocktails, 7-amino actinomycin D (7-AAD) and other general chemicals were from Sigma (St. Louis, MO); stock solutions of genistein, at 20 mM in dimethylsulfoxide (DMSO), were stored at −80°C before use. .. Primary HAECs were cultured in M199 medium containing 2% FBS and endothelial growth supplements-EGM2 at 37°C in a 5% CO2 /95% air environment.

    Activity Assay:

    Article Title: Pandemic Influenza A (H1N1) Virus Infection Increases Apoptosis and HIV-1 Replication in HIV-1 Infected Jurkat Cells
    Article Snippet: .. Caspase-3 Assay EnzChek® Caspase-3 Assay Kit #1, Z-DEVD-AMC Substrate from Invitrogen (Grand Island, NY, USA) was used to test caspase-3 activity, which was performed according to the manufacturer’s instruction. .. Cell Viability Assay Cell viability was determined by Trypan blue exclusion analysis (Life Technologies, Waltham, MA, USA).

    Labeling:

    Article Title: Isoflavone genistein protects human vascular endothelial cells against tumor necrosis factor-?-induced apoptosis through the p38? mitogen-activated protein kinase
    Article Snippet: .. Primary HAECs, fetal bovine serum (FBS) and endothelial growth supplements (EGM2) were purchased from Lonza Walkersville ( Walkersville, MD); M199 media, caspase-3 assay kits and competent cells for plasmid transformation were from Invitrogen (Carlsbad, CA); antibodies against p38, phospho-p38 (Thrl80/Tyrl82), p38α, p38β, Bcl-2 and β-actin were from Cell Signaling Technology (Beverly, MA); supersignal chemiluminescence detection system and Protein A beads were from Pierce (Rockford, IL); nitrocellulose membranes and protein assay kits were from Bio-Rad (Hercules, CA); Bcl-2 promoter-driven luciferase (Bcl-2-Luc) reporter construct was a kind gift from Dr. Linda M. Boxer, Stanford University, Stanford, CA); plasmid purification kit was from Qiagen (Valencia, CA); transfection reagents were from Targeting System (Santee, CA); dual luciferase reporter assay kits were from Promega (Madison, WI); terminal deoxynucleotidyltransferase dUTP nick-end labeling (TUNEL) and apoptotic DNA ladder kits were from Roche Applied Science (Indianapolis, IN); ICI182,780 was from Tocris (St. Louis, MO); genistein, TNF-α, SB203580, SB203474, H89, PD098059, protease and phosphatase inhibitor cocktails, 7-amino actinomycin D (7-AAD) and other general chemicals were from Sigma (St. Louis, MO); stock solutions of genistein, at 20 mM in dimethylsulfoxide (DMSO), were stored at −80°C before use. .. Primary HAECs were cultured in M199 medium containing 2% FBS and endothelial growth supplements-EGM2 at 37°C in a 5% CO2 /95% air environment.

    Expressing:

    Article Title: Antioxidant Activity of Zein Hydrolysates from Zea Species and Their Cytotoxic Effects in a Hepatic Cell Culture
    Article Snippet: .. We used 3 kits to evaluate caspase expression: caspase 3: EnzChek Caspase-3 Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA); caspase 8: ApoTarget Caspase-8 Fluorometric Protease Assay (Invitrogen, Frederick, MD, USA); and caspase 9: ApoTarget Caspase-9 Colorimetric Protease Assay (Invitrogen). ..

    Transformation Assay:

    Article Title: Isoflavone genistein protects human vascular endothelial cells against tumor necrosis factor-?-induced apoptosis through the p38? mitogen-activated protein kinase
    Article Snippet: .. Primary HAECs, fetal bovine serum (FBS) and endothelial growth supplements (EGM2) were purchased from Lonza Walkersville ( Walkersville, MD); M199 media, caspase-3 assay kits and competent cells for plasmid transformation were from Invitrogen (Carlsbad, CA); antibodies against p38, phospho-p38 (Thrl80/Tyrl82), p38α, p38β, Bcl-2 and β-actin were from Cell Signaling Technology (Beverly, MA); supersignal chemiluminescence detection system and Protein A beads were from Pierce (Rockford, IL); nitrocellulose membranes and protein assay kits were from Bio-Rad (Hercules, CA); Bcl-2 promoter-driven luciferase (Bcl-2-Luc) reporter construct was a kind gift from Dr. Linda M. Boxer, Stanford University, Stanford, CA); plasmid purification kit was from Qiagen (Valencia, CA); transfection reagents were from Targeting System (Santee, CA); dual luciferase reporter assay kits were from Promega (Madison, WI); terminal deoxynucleotidyltransferase dUTP nick-end labeling (TUNEL) and apoptotic DNA ladder kits were from Roche Applied Science (Indianapolis, IN); ICI182,780 was from Tocris (St. Louis, MO); genistein, TNF-α, SB203580, SB203474, H89, PD098059, protease and phosphatase inhibitor cocktails, 7-amino actinomycin D (7-AAD) and other general chemicals were from Sigma (St. Louis, MO); stock solutions of genistein, at 20 mM in dimethylsulfoxide (DMSO), were stored at −80°C before use. .. Primary HAECs were cultured in M199 medium containing 2% FBS and endothelial growth supplements-EGM2 at 37°C in a 5% CO2 /95% air environment.

    Plasmid Preparation:

    Article Title: Isoflavone genistein protects human vascular endothelial cells against tumor necrosis factor-?-induced apoptosis through the p38? mitogen-activated protein kinase
    Article Snippet: .. Primary HAECs, fetal bovine serum (FBS) and endothelial growth supplements (EGM2) were purchased from Lonza Walkersville ( Walkersville, MD); M199 media, caspase-3 assay kits and competent cells for plasmid transformation were from Invitrogen (Carlsbad, CA); antibodies against p38, phospho-p38 (Thrl80/Tyrl82), p38α, p38β, Bcl-2 and β-actin were from Cell Signaling Technology (Beverly, MA); supersignal chemiluminescence detection system and Protein A beads were from Pierce (Rockford, IL); nitrocellulose membranes and protein assay kits were from Bio-Rad (Hercules, CA); Bcl-2 promoter-driven luciferase (Bcl-2-Luc) reporter construct was a kind gift from Dr. Linda M. Boxer, Stanford University, Stanford, CA); plasmid purification kit was from Qiagen (Valencia, CA); transfection reagents were from Targeting System (Santee, CA); dual luciferase reporter assay kits were from Promega (Madison, WI); terminal deoxynucleotidyltransferase dUTP nick-end labeling (TUNEL) and apoptotic DNA ladder kits were from Roche Applied Science (Indianapolis, IN); ICI182,780 was from Tocris (St. Louis, MO); genistein, TNF-α, SB203580, SB203474, H89, PD098059, protease and phosphatase inhibitor cocktails, 7-amino actinomycin D (7-AAD) and other general chemicals were from Sigma (St. Louis, MO); stock solutions of genistein, at 20 mM in dimethylsulfoxide (DMSO), were stored at −80°C before use. .. Primary HAECs were cultured in M199 medium containing 2% FBS and endothelial growth supplements-EGM2 at 37°C in a 5% CO2 /95% air environment.

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    • cellevent caspase 3 7 green flow cytometry assay kit  (Thermo Fisher)
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    Thermo Fisher cellevent caspase 3 7 green flow cytometry assay kit
    AVN-C treatment activates caspases 3/7. a Representative sample of flow cytometric analysis of cells treated with <t>CellEvent</t> <t>Caspase-3/7</t> Green Flow Cytometric Assay Kit to detect caspase activity (Comp-FL1-A) and dead cells (Comp-FL3-A) after treatment
    Cellevent Caspase 3 7 Green Flow Cytometry Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    caspase 3 cleaved multispecies in cell elisa kit  (Thermo Fisher)
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    Thermo Fisher caspase 3 cleaved multispecies in cell elisa kit
    The combination of miR-542-3p and paclitaxel significantly downregulates Survivin, inhibits proliferation, and induces apoptosis of HCC1954 breast cancer cells in vivo The tumors obtained from the animal experiments were evaluated by IHC analysis of Ki67, Survivin and cleaved <t>caspase-3.</t> A , Data show the representative images of representative tumors with hematoxylin and eosin (H E) staining and immunostaining of Ki67, Survivin and cleaved caspase-3 (Cleaved Casp-3). B , The IHC slides were observed by two independent personnel. The tumor cells with positive staining of Ki67, Survivin, or cleaved caspase-3 were counted from three randomly selected areas in each slide. The three areas were first identified by scanning the entire slide at ×10 magnification, and then the positively stained cells were counted at ×20 magnification using an Olympus B×40 microscope (Tokyo, Japan). A minimum of 500 tumor cells were evaluated. If differences occurred between spot intensities, the most positive spot was considered. The evaluations were recorded as percentages of positively stained target cells in each field. The bar graphs show the mean percentages of positively stained cells in each field. Bars , SD.
    Caspase 3 Cleaved Multispecies In Cell Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    caspase 3 active elisa kit  (Thermo Fisher)
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    Thermo Fisher caspase 3 active elisa kit
    Apoptosis induction in HCT116 and SW620 cells by PS-PDT The apoptotic rate in HCT116 and SW620 cells was determined 24 h after treatment with various doses of light irradiation with a fixed PS-II (10 μg/ml) ( A ) or different concentrations of PS-II with a fixed intensity (5 J/cm 2 ) ( B ). ( C ) FACS analysis of apoptosis in HCT116 and SW620 cells after treated with 10 μg/ml PS-II and 5 J/cm 2 light irradiation. ( D ) Hoechst staining and analysis of the condensed nuclei by fluorescence microscopy (200 × magnification). Apoptotic cells are shown in blue and the peripherally clumped or fragmented chromatin is indicated by arrows. ( E ) Western blot analysis of cleaved <t>Caspase-3,</t> Bax, cleaved PARP and Cyto-c (cytochrome C). β-actin was used as a control. ( F ) The relative activity of caspase-3 was detected in different groups by using <t>ELISA</t> kit. Data are expressed as mean ± sd of three independent experiments. *p
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    Image Search Results


    AVN-C treatment activates caspases 3/7. a Representative sample of flow cytometric analysis of cells treated with CellEvent Caspase-3/7 Green Flow Cytometric Assay Kit to detect caspase activity (Comp-FL1-A) and dead cells (Comp-FL3-A) after treatment

    Journal: Cancer Cell International

    Article Title: Avenanthramide-C reduces the viability of MDA-MB-231 breast cancer cells through an apoptotic mechanism

    doi: 10.1186/s12935-017-0464-0

    Figure Lengend Snippet: AVN-C treatment activates caspases 3/7. a Representative sample of flow cytometric analysis of cells treated with CellEvent Caspase-3/7 Green Flow Cytometric Assay Kit to detect caspase activity (Comp-FL1-A) and dead cells (Comp-FL3-A) after treatment

    Article Snippet: CellEvent Caspase 3/7 Green Flow Cytometry Assay Kit was purchased from Thermo Fisher Scientific (Waltham, MA) ( ).

    Techniques: Flow Cytometry, Activity Assay

    The combination of miR-542-3p and paclitaxel significantly downregulates Survivin, inhibits proliferation, and induces apoptosis of HCC1954 breast cancer cells in vivo The tumors obtained from the animal experiments were evaluated by IHC analysis of Ki67, Survivin and cleaved caspase-3. A , Data show the representative images of representative tumors with hematoxylin and eosin (H E) staining and immunostaining of Ki67, Survivin and cleaved caspase-3 (Cleaved Casp-3). B , The IHC slides were observed by two independent personnel. The tumor cells with positive staining of Ki67, Survivin, or cleaved caspase-3 were counted from three randomly selected areas in each slide. The three areas were first identified by scanning the entire slide at ×10 magnification, and then the positively stained cells were counted at ×20 magnification using an Olympus B×40 microscope (Tokyo, Japan). A minimum of 500 tumor cells were evaluated. If differences occurred between spot intensities, the most positive spot was considered. The evaluations were recorded as percentages of positively stained target cells in each field. The bar graphs show the mean percentages of positively stained cells in each field. Bars , SD.

    Journal: Cancer letters

    Article Title: Survivin-targeting miR-542-3p overcomes HER3 signaling-induced chemoresistance and enhances the antitumor activity of paclitaxel against HER2-overexpressing breast cancer

    doi: 10.1016/j.canlet.2018.01.065

    Figure Lengend Snippet: The combination of miR-542-3p and paclitaxel significantly downregulates Survivin, inhibits proliferation, and induces apoptosis of HCC1954 breast cancer cells in vivo The tumors obtained from the animal experiments were evaluated by IHC analysis of Ki67, Survivin and cleaved caspase-3. A , Data show the representative images of representative tumors with hematoxylin and eosin (H E) staining and immunostaining of Ki67, Survivin and cleaved caspase-3 (Cleaved Casp-3). B , The IHC slides were observed by two independent personnel. The tumor cells with positive staining of Ki67, Survivin, or cleaved caspase-3 were counted from three randomly selected areas in each slide. The three areas were first identified by scanning the entire slide at ×10 magnification, and then the positively stained cells were counted at ×20 magnification using an Olympus B×40 microscope (Tokyo, Japan). A minimum of 500 tumor cells were evaluated. If differences occurred between spot intensities, the most positive spot was considered. The evaluations were recorded as percentages of positively stained target cells in each field. The bar graphs show the mean percentages of positively stained cells in each field. Bars , SD.

    Article Snippet: For Ki67 and cleaved caspase-3, human tonsil tissues were used as positive controls.

    Techniques: In Vivo, Immunohistochemistry, Staining, Immunostaining, Microscopy

    The mimic of miR-542-3p effectively downregulates Survivin and significantly enhances paclitaxel-induced apoptosis in HER2-positive breast cancer cells SKBR3.B3.1 or SKBR3.B3.2 cells were transfected with the negative control miRNA mimic (Control, 40 nmol/L) or the mimic of miR-203 (40 nmol/L) or miR-542-3p (10 nmol/L) for 24 h. A , Cells were collected for western blot analyses of Survivin, Mcl-1, Bcl-xL, or β-actin. B C , The cells were then untreated or treated with paclitaxel (3 nmol/L) for another 24 h and subjected to western blot analyses of PARP, cleaved caspase-3 (C-Casp-3), or β-actin ( B ) or a specific apoptosis ELISA ( C ). Bars , S.D. Data represent the results of three independent experiments.

    Journal: Cancer letters

    Article Title: Survivin-targeting miR-542-3p overcomes HER3 signaling-induced chemoresistance and enhances the antitumor activity of paclitaxel against HER2-overexpressing breast cancer

    doi: 10.1016/j.canlet.2018.01.065

    Figure Lengend Snippet: The mimic of miR-542-3p effectively downregulates Survivin and significantly enhances paclitaxel-induced apoptosis in HER2-positive breast cancer cells SKBR3.B3.1 or SKBR3.B3.2 cells were transfected with the negative control miRNA mimic (Control, 40 nmol/L) or the mimic of miR-203 (40 nmol/L) or miR-542-3p (10 nmol/L) for 24 h. A , Cells were collected for western blot analyses of Survivin, Mcl-1, Bcl-xL, or β-actin. B C , The cells were then untreated or treated with paclitaxel (3 nmol/L) for another 24 h and subjected to western blot analyses of PARP, cleaved caspase-3 (C-Casp-3), or β-actin ( B ) or a specific apoptosis ELISA ( C ). Bars , S.D. Data represent the results of three independent experiments.

    Article Snippet: For Ki67 and cleaved caspase-3, human tonsil tissues were used as positive controls.

    Techniques: Transfection, Negative Control, Western Blot, Enzyme-linked Immunosorbent Assay

    Apoptosis induction in HCT116 and SW620 cells by PS-PDT The apoptotic rate in HCT116 and SW620 cells was determined 24 h after treatment with various doses of light irradiation with a fixed PS-II (10 μg/ml) ( A ) or different concentrations of PS-II with a fixed intensity (5 J/cm 2 ) ( B ). ( C ) FACS analysis of apoptosis in HCT116 and SW620 cells after treated with 10 μg/ml PS-II and 5 J/cm 2 light irradiation. ( D ) Hoechst staining and analysis of the condensed nuclei by fluorescence microscopy (200 × magnification). Apoptotic cells are shown in blue and the peripherally clumped or fragmented chromatin is indicated by arrows. ( E ) Western blot analysis of cleaved Caspase-3, Bax, cleaved PARP and Cyto-c (cytochrome C). β-actin was used as a control. ( F ) The relative activity of caspase-3 was detected in different groups by using ELISA kit. Data are expressed as mean ± sd of three independent experiments. *p

    Journal: Oncotarget

    Article Title: Autophagy inhibition enhances photocytotoxicity of Photosan-II in human colorectal cancer cells

    doi: 10.18632/oncotarget.14117

    Figure Lengend Snippet: Apoptosis induction in HCT116 and SW620 cells by PS-PDT The apoptotic rate in HCT116 and SW620 cells was determined 24 h after treatment with various doses of light irradiation with a fixed PS-II (10 μg/ml) ( A ) or different concentrations of PS-II with a fixed intensity (5 J/cm 2 ) ( B ). ( C ) FACS analysis of apoptosis in HCT116 and SW620 cells after treated with 10 μg/ml PS-II and 5 J/cm 2 light irradiation. ( D ) Hoechst staining and analysis of the condensed nuclei by fluorescence microscopy (200 × magnification). Apoptotic cells are shown in blue and the peripherally clumped or fragmented chromatin is indicated by arrows. ( E ) Western blot analysis of cleaved Caspase-3, Bax, cleaved PARP and Cyto-c (cytochrome C). β-actin was used as a control. ( F ) The relative activity of caspase-3 was detected in different groups by using ELISA kit. Data are expressed as mean ± sd of three independent experiments. *p

    Article Snippet: Caspase-3 (active) ELISA Kit, Human was purchased from thermofisher scientific (KHO1091).

    Techniques: Irradiation, FACS, Staining, Fluorescence, Microscopy, Western Blot, Activity Assay, Enzyme-linked Immunosorbent Assay