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casp9  (OriGene)
94
OriGene casp9
Long-term exposure to 5.0% ethanol triggers apoptosis, which undermines cell growth. A . An equal number of HEEC cells was seeded in 6-well plates and incubated with the complete medium containing DMSO (control), the medium containing 5.0% ethanol (EtOH), the medium containing 2 µM Z-LEHD-FMK (LEHD), or the medium containing 5.0% ethanol plus 2 µM LEHD for 30 min. Protein was extracted and analyzed by Western blotting. The membranes were probed for CASP8, <t>CASP9,</t> CASP3, or GAPDH. B . Quantitative analyses of CASP8, CASP9, and CASP3 expression against GAPDH in HEEC cells. * indicates a significant change compared to the control (DMSO). C . An equal number of HEEC cells was seeded on the coverslips and incubated with the complete medium (control) or the medium containing 5.0% ethanol for 5–30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for endoG. An FITC-conjugated secondary antibody was used to develop the signal. Cell nuclei were counterstained with DAPI. Scale bar = 5 μm. D . Quantitative analyses of endoG nuclear translocation in HEEC cells (%). * indicates a significant change compared to the control. E . An equal number of HEEC cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD. F . An equal number of Het1A cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD
Casp9, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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casp9  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc casp9
Long-term exposure to 5.0% ethanol triggers apoptosis, which undermines cell growth. A . An equal number of HEEC cells was seeded in 6-well plates and incubated with the complete medium containing DMSO (control), the medium containing 5.0% ethanol (EtOH), the medium containing 2 µM Z-LEHD-FMK (LEHD), or the medium containing 5.0% ethanol plus 2 µM LEHD for 30 min. Protein was extracted and analyzed by Western blotting. The membranes were probed for CASP8, <t>CASP9,</t> CASP3, or GAPDH. B . Quantitative analyses of CASP8, CASP9, and CASP3 expression against GAPDH in HEEC cells. * indicates a significant change compared to the control (DMSO). C . An equal number of HEEC cells was seeded on the coverslips and incubated with the complete medium (control) or the medium containing 5.0% ethanol for 5–30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for endoG. An FITC-conjugated secondary antibody was used to develop the signal. Cell nuclei were counterstained with DAPI. Scale bar = 5 μm. D . Quantitative analyses of endoG nuclear translocation in HEEC cells (%). * indicates a significant change compared to the control. E . An equal number of HEEC cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD. F . An equal number of Het1A cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD
Casp9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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casp9  (Abmart Inc)
86
Abmart Inc casp9
Long-term exposure to 5.0% ethanol triggers apoptosis, which undermines cell growth. A . An equal number of HEEC cells was seeded in 6-well plates and incubated with the complete medium containing DMSO (control), the medium containing 5.0% ethanol (EtOH), the medium containing 2 µM Z-LEHD-FMK (LEHD), or the medium containing 5.0% ethanol plus 2 µM LEHD for 30 min. Protein was extracted and analyzed by Western blotting. The membranes were probed for CASP8, <t>CASP9,</t> CASP3, or GAPDH. B . Quantitative analyses of CASP8, CASP9, and CASP3 expression against GAPDH in HEEC cells. * indicates a significant change compared to the control (DMSO). C . An equal number of HEEC cells was seeded on the coverslips and incubated with the complete medium (control) or the medium containing 5.0% ethanol for 5–30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for endoG. An FITC-conjugated secondary antibody was used to develop the signal. Cell nuclei were counterstained with DAPI. Scale bar = 5 μm. D . Quantitative analyses of endoG nuclear translocation in HEEC cells (%). * indicates a significant change compared to the control. E . An equal number of HEEC cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD. F . An equal number of Het1A cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD
Casp9, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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pmscv f del casp9 ires gfp  (Addgene inc)
93
Addgene inc pmscv f del casp9 ires gfp
Long-term exposure to 5.0% ethanol triggers apoptosis, which undermines cell growth. A . An equal number of HEEC cells was seeded in 6-well plates and incubated with the complete medium containing DMSO (control), the medium containing 5.0% ethanol (EtOH), the medium containing 2 µM Z-LEHD-FMK (LEHD), or the medium containing 5.0% ethanol plus 2 µM LEHD for 30 min. Protein was extracted and analyzed by Western blotting. The membranes were probed for CASP8, <t>CASP9,</t> CASP3, or GAPDH. B . Quantitative analyses of CASP8, CASP9, and CASP3 expression against GAPDH in HEEC cells. * indicates a significant change compared to the control (DMSO). C . An equal number of HEEC cells was seeded on the coverslips and incubated with the complete medium (control) or the medium containing 5.0% ethanol for 5–30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for endoG. An FITC-conjugated secondary antibody was used to develop the signal. Cell nuclei were counterstained with DAPI. Scale bar = 5 μm. D . Quantitative analyses of endoG nuclear translocation in HEEC cells (%). * indicates a significant change compared to the control. E . An equal number of HEEC cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD. F . An equal number of Het1A cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD
Pmscv F Del Casp9 Ires Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
pmscv f del casp9 ires gfp - by Bioz Stars, 2026-08
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icasp9 ha sequence  (Addgene inc)
93
Addgene inc icasp9 ha sequence
Long-term exposure to 5.0% ethanol triggers apoptosis, which undermines cell growth. A . An equal number of HEEC cells was seeded in 6-well plates and incubated with the complete medium containing DMSO (control), the medium containing 5.0% ethanol (EtOH), the medium containing 2 µM Z-LEHD-FMK (LEHD), or the medium containing 5.0% ethanol plus 2 µM LEHD for 30 min. Protein was extracted and analyzed by Western blotting. The membranes were probed for CASP8, <t>CASP9,</t> CASP3, or GAPDH. B . Quantitative analyses of CASP8, CASP9, and CASP3 expression against GAPDH in HEEC cells. * indicates a significant change compared to the control (DMSO). C . An equal number of HEEC cells was seeded on the coverslips and incubated with the complete medium (control) or the medium containing 5.0% ethanol for 5–30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for endoG. An FITC-conjugated secondary antibody was used to develop the signal. Cell nuclei were counterstained with DAPI. Scale bar = 5 μm. D . Quantitative analyses of endoG nuclear translocation in HEEC cells (%). * indicates a significant change compared to the control. E . An equal number of HEEC cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD. F . An equal number of Het1A cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD
Icasp9 Ha Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/casp9/pm41819100-326-24-30?v=Addgene+inc
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icasp9 ha sequence - by Bioz Stars, 2026-08
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addgene pmscv f del casp9 ires gfp  (Addgene inc)
93
Addgene inc addgene pmscv f del casp9 ires gfp
Long-term exposure to 5.0% ethanol triggers apoptosis, which undermines cell growth. A . An equal number of HEEC cells was seeded in 6-well plates and incubated with the complete medium containing DMSO (control), the medium containing 5.0% ethanol (EtOH), the medium containing 2 µM Z-LEHD-FMK (LEHD), or the medium containing 5.0% ethanol plus 2 µM LEHD for 30 min. Protein was extracted and analyzed by Western blotting. The membranes were probed for CASP8, <t>CASP9,</t> CASP3, or GAPDH. B . Quantitative analyses of CASP8, CASP9, and CASP3 expression against GAPDH in HEEC cells. * indicates a significant change compared to the control (DMSO). C . An equal number of HEEC cells was seeded on the coverslips and incubated with the complete medium (control) or the medium containing 5.0% ethanol for 5–30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for endoG. An FITC-conjugated secondary antibody was used to develop the signal. Cell nuclei were counterstained with DAPI. Scale bar = 5 μm. D . Quantitative analyses of endoG nuclear translocation in HEEC cells (%). * indicates a significant change compared to the control. E . An equal number of HEEC cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD. F . An equal number of Het1A cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD
Addgene Pmscv F Del Casp9 Ires Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/casp9/pm41819100-325-6-6?v=Addgene+inc
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addgene pmscv f del casp9 ires gfp - by Bioz Stars, 2026-08
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addgene plasmid  (Addgene inc)
93
Addgene inc addgene plasmid
Long-term exposure to 5.0% ethanol triggers apoptosis, which undermines cell growth. A . An equal number of HEEC cells was seeded in 6-well plates and incubated with the complete medium containing DMSO (control), the medium containing 5.0% ethanol (EtOH), the medium containing 2 µM Z-LEHD-FMK (LEHD), or the medium containing 5.0% ethanol plus 2 µM LEHD for 30 min. Protein was extracted and analyzed by Western blotting. The membranes were probed for CASP8, <t>CASP9,</t> CASP3, or GAPDH. B . Quantitative analyses of CASP8, CASP9, and CASP3 expression against GAPDH in HEEC cells. * indicates a significant change compared to the control (DMSO). C . An equal number of HEEC cells was seeded on the coverslips and incubated with the complete medium (control) or the medium containing 5.0% ethanol for 5–30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for endoG. An FITC-conjugated secondary antibody was used to develop the signal. Cell nuclei were counterstained with DAPI. Scale bar = 5 μm. D . Quantitative analyses of endoG nuclear translocation in HEEC cells (%). * indicates a significant change compared to the control. E . An equal number of HEEC cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD. F . An equal number of Het1A cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD
Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/casp9/pm41819100-261-30-30?v=Addgene+inc
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Long-term exposure to 5.0% ethanol triggers apoptosis, which undermines cell growth. A . An equal number of HEEC cells was seeded in 6-well plates and incubated with the complete medium containing DMSO (control), the medium containing 5.0% ethanol (EtOH), the medium containing 2 µM Z-LEHD-FMK (LEHD), or the medium containing 5.0% ethanol plus 2 µM LEHD for 30 min. Protein was extracted and analyzed by Western blotting. The membranes were probed for CASP8, CASP9, CASP3, or GAPDH. B . Quantitative analyses of CASP8, CASP9, and CASP3 expression against GAPDH in HEEC cells. * indicates a significant change compared to the control (DMSO). C . An equal number of HEEC cells was seeded on the coverslips and incubated with the complete medium (control) or the medium containing 5.0% ethanol for 5–30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for endoG. An FITC-conjugated secondary antibody was used to develop the signal. Cell nuclei were counterstained with DAPI. Scale bar = 5 μm. D . Quantitative analyses of endoG nuclear translocation in HEEC cells (%). * indicates a significant change compared to the control. E . An equal number of HEEC cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD. F . An equal number of Het1A cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD

Journal: BMC Molecular and Cell Biology

Article Title: Alcohol exposure induces ferroptosis-dominated programmed cell death in esophageal epithelial cells

doi: 10.1186/s12860-026-00589-5

Figure Lengend Snippet: Long-term exposure to 5.0% ethanol triggers apoptosis, which undermines cell growth. A . An equal number of HEEC cells was seeded in 6-well plates and incubated with the complete medium containing DMSO (control), the medium containing 5.0% ethanol (EtOH), the medium containing 2 µM Z-LEHD-FMK (LEHD), or the medium containing 5.0% ethanol plus 2 µM LEHD for 30 min. Protein was extracted and analyzed by Western blotting. The membranes were probed for CASP8, CASP9, CASP3, or GAPDH. B . Quantitative analyses of CASP8, CASP9, and CASP3 expression against GAPDH in HEEC cells. * indicates a significant change compared to the control (DMSO). C . An equal number of HEEC cells was seeded on the coverslips and incubated with the complete medium (control) or the medium containing 5.0% ethanol for 5–30 min, or 2, 6, and 12 h. Cells were fixed in 4% paraformaldehyde and stained for endoG. An FITC-conjugated secondary antibody was used to develop the signal. Cell nuclei were counterstained with DAPI. Scale bar = 5 μm. D . Quantitative analyses of endoG nuclear translocation in HEEC cells (%). * indicates a significant change compared to the control. E . An equal number of HEEC cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD. F . An equal number of Het1A cells was seeded in 96-well plates and incubated with the medium containing 5.0% ethanol (EtOH) or the medium containing 5.0% ethanol plus 2 µM LEHD for 5 min, 30 min, 2 h, 6 h, or 12 h. Cell viability was assessed using CCK-8 kits. * indicates a significant change between EtOH and EtOH/LEHD

Article Snippet: The following primary antibodies were used: Beclin-1 (Origene, #TA502643), PCNA, CASP1 (Abcam, #ab179515), CASP3 (Santa Cruz Biotechnology, #sc-271759), CASP8 (Origene, #TA374288), CASP9 (Origene, #TA4227045), endoG, GPX4 (Origene, #TA423164M), GSDMD, IL-1β (Santa Cruz Biotechnology, #sc-12742), MLKL, phosphor-MLKL (Abcam, #ab196436), SLC7A11 (Origene, #TA423232), LC3B, BAX, GAPDH (OriGene, #TA800894), and β-actin (Santa Cruz Biotechnology, Inc. #sc-69879).

Techniques: Incubation, Control, Western Blot, Expressing, Staining, Translocation Assay, CCK-8 Assay