Journal: Frontiers in Pharmacology

Article Title: Anti-Myocardial Ischemia Reperfusion Injury Mechanism of Dried Ginger-Aconite Decoction Based on Network Pharmacology

doi: 10.3389/fphar.2021.609702

Figure Lengend Snippet: Experimental validation of key signaling pathways and mitochondrial key targets in vivo . Dried ginger-aconite decoction(DAD) activates the PI3k/Akt/GSK-3β signaling pathway and inhibits the expression of Cyt-C and CASP9. Data were presented as mean standard deviation (SD) of three independent experiments. # p < 0.05, ## p < 0.01 vs. sham group. * p < 0.05, ** p < 0.01 vs. MI/RI group.

Article Snippet: Cyt-C, β -action, GADPH, Casp9, PI3K, AKT, Bax, Bcl-2 and phosphorylated( P )-AKT, GSK3β were also purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Biomarker Discovery, Protein-Protein interactions, In Vivo, Expressing, Standard Deviation

Journal: Cancer Cell International

Article Title: Silencing Uracil-DNA glycosylase inhibits colorectal cancer progression

doi: 10.1186/s12935-025-04089-y

Figure Lengend Snippet: UNG activated several signaling pathways in CRC cells. Western blot was performed to detect the levels of mTOR, p-mTOR, p70 S6K, p-P70 S6K, AKT, p-AKT, AMPK, p-AMPK, ERK, p-ERK, Bax, Bcl2, cleavages of caspase-9, and caspase-3 in UNG-knockdown CRC cells. Fold changes (Fc) are shown below the bars

Article Snippet: The membranes were incubated overnight at 4 °C with the following primary antibodies: UNG, P70 S6K, p-P70 S6K(S424) (1:1000, Bioworld), β-actin (1:1000, Abcam), cleaved caspase-3, AMPKα1/AMPKα2, p-AMPKα1(Thr183)/AMPKα2(Thr172) (1:1000, Beyotime), BAX, BCL2, AKT1/2/3, mTOR, and p-mTOR (Ser2448) (1:1000; BOSTER), cleaved caspase-9, p-AKT(Ser473), ERK1/2, and p-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) (1:1000; ZEN BIO), and GAPDH (1:1000; Proteintech).

Techniques: Protein-Protein interactions, Western Blot, Knockdown

Journal: International Journal of Molecular Sciences

Article Title: Therapeutic Efficiency of Proteins Secreted by Glial Progenitor Cells in a Rat Model of Traumatic Brain Injury

doi: 10.3390/ijms241512341

Figure Lengend Snippet: Immunoblotting for caspase-9: ( A ) representative blots, ( B ) semi-quantitation data presented as means ± SD; * p < 0.05 ( t -test).

Article Snippet: The membranes were pre-blocked overnight with 3% BSA in PBS and incubated with primary antibodies to p-Tau Ser396 (1:1000, ab32057), p-Tau Thr205 (1:1000, ab254410), APP (1:1000, ab32136) (all by Abcam, UK), Tau (1:1000, 46687) (Cell Signaling, Danvers, MA, USA), and β-actin (1:1000, 8457) (Cell Signaling) for neurodegeneration marker analysis and β-actin (1:2000, A1978) (Sigma-Aldrich) and cleaved-CASP9 (D353) antibody (1:500, CSB-PA000010) (Cusabio, Wuhan, China) for caspase-9 analysis in PBS with 0.1% Tween-20 and 1% BSA.

Techniques: Western Blot, Quantitation Assay

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: The Regulation of Trophoblastic p53 Homeostasis by the p38-Wip1 Feedback Loop is Disturbed in Placentas from Pregnancies Complicated by Preeclampsia.

doi: 10.33594/000000023

Figure Lengend Snippet: Fig. 2. Wip1 activates p38 and dephosphorylates p53 in trophoblasts under conditions of hypoxia. A: Lentiviral transfection efficiency was examined by FCM and IF. Scale bar 400 µm; B: Wip1 protein expression was confirmed by western blotting, n=4, *P<0.05 vs. scrambled shRNA, one-way ANOVA, Dunnett-t multiple comparison test; C-D. Representative Western blots of p-p38Thr180/Tyr182, p38, cl-cas9, Mdm2, p-p53Ser15, and p53 in HTR8/SVneo cells after 24 h of HII (A) or SIB (B) in the presence of 20 µM GSK2830371 (GSK), DMSO (0.1%), shWip1, or scrambled shRNA (MOI=50). n=4, (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, one- way ANOVA, Sidak’s multiple comparisons test. Experiments were performed in triplicate.

Article Snippet: KG Tan et al.: p38-Wip1 Loop Regulates p53 in Trophoblast p38 (1:1000), and cleaved caspase-9 (cl-cas9, 1:1000) were purchased from Cell Signaling Technology (Danvers, USA); p53 (1:2000) and β-actin (1:1000) were purchased from Proteintech (Rosemont, USA); Wip1 (1:1000) and Mdm2 (1:1000) were purchased from Abcam (Cambridge, UK) and Invitrogen (Waltham, USA), respectively.

Techniques: Transfection, Expressing, Western Blot, shRNA, Comparison