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Waters Corporation cartridge
Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cartridge/product/Waters Corporation
Average 94 stars, based on 3 article reviews
Price from $9.99 to $1999.99
cartridge - by Bioz Stars, 2020-04
94/100 stars

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Stable Transfection:

Article Title: Probing the Production of Amidated Peptides following Genetic and Dietary Copper Manipulations
Article Snippet: Synthetic ACTH(1-13)-Gly [ACTH(1-14)] (AnaSpec, Fremont, CA, USA) was converted into ACTH(1-13)-Gly-OH using PHM catalytic core purified from stably transfected Chinese hamster ovary cell medium , , . .. The peptide was adsorbed to a SepPak C18 μBondapak cartridge (Waters Corporation, Milford, MA, USA), which was then washed with 0.1% TFA and eluted with 80% ACN in 0.1% TFA.

Synthesized:

Article Title: Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells
Article Snippet: Peptides 9-fluorenylmethoxycarbonyl (Fmoc)–protected dimethoxy-nitrobenzyl (DMNB) Ser was synthesized essentially as previously described ( ). .. After allyl deprotection with palladium and purification by silica gel chromatography, the Fmoc-Ser(DMNB)-OH was further purified over a cartridge (Sep-Pak C18; Waters) using a reversed-phase solvent system: 0.1% aqueous trifluoroacetic acid (solvent A) versus 90% acetonitrile plus 0.1% trifluoroacetic acid (solvent B).

Incubation:

Article Title: KSHV oral shedding and plasma viremia result in significant changes in the extracellular tumorigenic miRNA expression profile in individuals infected with the malaria parasite
Article Snippet: One of 10 TMT™ mass tag labeling reagents, 0.8mg (Thermo Scientific, Waltham, MA) was then added in 40 μL of acetonitrile to each vial, and the 10 vials were incubated at room temperature for 1 hour. .. The pH was adjusted to less than 3 with 2% TFA right before desalting using Oasis HLB 10mg cartridge (Waters, Milford, MA) and stored at -20°C until fractionation by strong cation-exchange (SCX) chromatography.

Article Title: GPR158/179 regulate G protein signaling by controlling localization and activity of the RGS7 complexes
Article Snippet: Samples were centrifuged (30,000 g for 20 min) to remove debris, and supernatants were incubated for 45 min with 5 mg anti-RGS7 antibody covalently coupled to protein G beads as previously described ( ). .. Proteins were digested with 20 µg of modified porcine trypsin (Promega) in the presence of 3 mM CaCl2 at 37°C for 16 h. Peptide mixtures were vacuum dried, reconstituted in 0.2% formic acid (Thermo Fisher Scientific), and applied to a cartridge (Oasis MCX; Waters) preequilibrated with methanol/water (1:1 vol/vol).

Article Title: EPSIN 3, A Novel p53 Target, Regulates the Apoptotic Pathway and Gastric Carcinogenesis
Article Snippet: .. Trypsin GOLD solution was added at a ratio of 1/50 (w/w) enzyme to protein and incubated at 37°C for 16 h. The resulting peptides were desalted with an Oasis HLB 1 ml cartridge (Waters) and dried with a vacuum spin dryer. .. To enrich glycopeptides, peptides were dissolved in PBS and incubated with 50 μl of Con-A agarose beads (Seikagaku Corporation, Japan) at 25°C for 60 min.

Article Title: Early Responses to Severe Drought Stress in the Arabidopsis thaliana Cell Suspension Culture Proteome
Article Snippet: Reduction with 10 mM dithiothreitol in HEPES (pH 8.0) was applied and incubation took place for one hour at 37 °C, followed by alkylation with 25 mM iodoacetamide in HEPES (pH 8.0), incubation for two hours in dark and precipitation with 10 volumes of cold acetone overnight −20 °C. .. In a final step, the pooled TMT labelled peptides were desalted using Sep-Pak Vac tC18 100 mg cartridge (Waters, Milford, MA, USA).

Article Title: Proteomics and Phosphoproteomics Analysis of Human Lens Fiber Cell Membranes
Article Snippet: The remaining pellets were suspended in 100 μL 10 mM of HCl and 2 μg of sequencing-grade pepsin (Princeton Separation) was added and the sample was incubated at 37°C for 6 hours. .. The ACN extracts and the initially collected supernatants were pooled together and diluted 5 times by 0.1% formic acid and loaded onto a cartridge (SepPak C18; Waters, Milford, MA).

Mass Spectrometry:

Article Title: GPR158/179 regulate G protein signaling by controlling localization and activity of the RGS7 complexes
Article Snippet: Paragraph title: Preparative immunoprecipitation and mass spectrometry ... Proteins were digested with 20 µg of modified porcine trypsin (Promega) in the presence of 3 mM CaCl2 at 37°C for 16 h. Peptide mixtures were vacuum dried, reconstituted in 0.2% formic acid (Thermo Fisher Scientific), and applied to a cartridge (Oasis MCX; Waters) preequilibrated with methanol/water (1:1 vol/vol).

Article Title: A Comparative Proteomic Analysis of the KATP channel Complex in Different Tissue Types
Article Snippet: For all LC-MSE experiments, a QTof-Premier (Waters) coupled to a nanoAcquity Ultra Performance LC (Waters) with a 5 µm Acclaim PepMap100 C18 µ-precolumn cartridge (300 µm × 5 mm; LC Packings) and a 1.7 µm BEH130 C18 analytical column (100 µm × 100 µm; Waters). .. For other LC-MS/MS experiments, an LTQ-Orbitrap hybrid mass spectrometer (Thermo Fisher Scientific) with a nanoelectrospray ionization source (Jamie Hill Instrument Services) coupled to an Eksigent nanoLC system (Eksigent Technologies) with a self-packed 100 mm × 15 cm reverse phase column (Reprosil C18, 3 mm, Dr. Maisch GmbH, Germany) was used.

Article Title: Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells
Article Snippet: After allyl deprotection with palladium and purification by silica gel chromatography, the Fmoc-Ser(DMNB)-OH was further purified over a cartridge (Sep-Pak C18; Waters) using a reversed-phase solvent system: 0.1% aqueous trifluoroacetic acid (solvent A) versus 90% acetonitrile plus 0.1% trifluoroacetic acid (solvent B). .. Purified Fmoc-Ser(DMNB)-OH was validated by electrospray mass spectrometry and nuclear magnetic resonance, and it was then incorporated into a modified importin-α peptide at the p8 position by solid-phase peptide synthesis.

Modification:

Article Title: GPR158/179 regulate G protein signaling by controlling localization and activity of the RGS7 complexes
Article Snippet: .. Proteins were digested with 20 µg of modified porcine trypsin (Promega) in the presence of 3 mM CaCl2 at 37°C for 16 h. Peptide mixtures were vacuum dried, reconstituted in 0.2% formic acid (Thermo Fisher Scientific), and applied to a cartridge (Oasis MCX; Waters) preequilibrated with methanol/water (1:1 vol/vol). ..

Article Title: EPSIN 3, A Novel p53 Target, Regulates the Apoptotic Pathway and Gastric Carcinogenesis
Article Snippet: Trypsin GOLD solution was added at a ratio of 1/50 (w/w) enzyme to protein and incubated at 37°C for 16 h. The resulting peptides were desalted with an Oasis HLB 1 ml cartridge (Waters) and dried with a vacuum spin dryer. .. Following 4 washes with PBS, N-glycosylated peptides were specifically eluted by incubation with 1 unit of PNGase-F solution (Sigma) at 37°C for 6 h. The released de-glycosylated peptides were finally analyzed with an LTQ-Orbitrap-Velos LC/MS system and subjected to database search analysis in which amino acid substitution (Asn > Asp) was added to the dynamic modification setting in Proteome Discoverer software to determine de-glycosylation sites generated by PNGase-F.

Article Title: Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells
Article Snippet: After allyl deprotection with palladium and purification by silica gel chromatography, the Fmoc-Ser(DMNB)-OH was further purified over a cartridge (Sep-Pak C18; Waters) using a reversed-phase solvent system: 0.1% aqueous trifluoroacetic acid (solvent A) versus 90% acetonitrile plus 0.1% trifluoroacetic acid (solvent B). .. Purified Fmoc-Ser(DMNB)-OH was validated by electrospray mass spectrometry and nuclear magnetic resonance, and it was then incorporated into a modified importin-α peptide at the p8 position by solid-phase peptide synthesis.

High Performance Liquid Chromatography:

Article Title: Probing the Production of Amidated Peptides following Genetic and Dietary Copper Manipulations
Article Snippet: Materials HPLC grade solvents such as water and acetonitrile (ACN) with 0.1% formic acid (FA) and 0.01% trifluoroacetic acid (TFA) were purchased from Fisher Scientific (Pittsburgh, PA, USA). .. The peptide was adsorbed to a SepPak C18 μBondapak cartridge (Waters Corporation, Milford, MA, USA), which was then washed with 0.1% TFA and eluted with 80% ACN in 0.1% TFA.

Article Title: Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells
Article Snippet: After allyl deprotection with palladium and purification by silica gel chromatography, the Fmoc-Ser(DMNB)-OH was further purified over a cartridge (Sep-Pak C18; Waters) using a reversed-phase solvent system: 0.1% aqueous trifluoroacetic acid (solvent A) versus 90% acetonitrile plus 0.1% trifluoroacetic acid (solvent B). .. After trifluoroacetic acid–mediated cleavage from the resin, the caged peptide was purified by reversed-phase HPLC using a C18 column in the absence of UV detection.

Flow Cytometry:

Article Title: An assessment of the concentrations of pharmaceutical compounds in wastewater treatment plants on the island of Gran Canaria (Spain)
Article Snippet: The cartridges were dried under vacuum for 10 minutes, and the analytes were eluted at an approximate flow rate of 1 mL · min-1 . .. For Groups 1 and 2, the SPE cartridge used was an OASIS HLB 6 mL/200 mg cartridge (Waters, Spain).

Article Title: KSHV oral shedding and plasma viremia result in significant changes in the extracellular tumorigenic miRNA expression profile in individuals infected with the malaria parasite
Article Snippet: The pH was adjusted to less than 3 with 2% TFA right before desalting using Oasis HLB 10mg cartridge (Waters, Milford, MA) and stored at -20°C until fractionation by strong cation-exchange (SCX) chromatography. .. The pooled sample was dissolved in buffer A (7 mM potassium phosphate, 30% acetonitrile, pH 2.65) immediately before fractionation with a SCX column (PolySulfoethy A, 5 μm, 2.1 × 100 mm, PolyLC Inc., Columbia, MD) The fractions were collected every minute at a flow rate of 200 μL/min from 1% solvent B (7 mM potassium phosphate, 500 mM KCl, 30% acetonitrile, pH 2.65) to 60% over 40 minutes (1% B for 7 minutes, 6–15% B for 18 minutes, 15–34% B for 10 minutes, and 34–60% B for 5 minutes) as well as during column washing with 98% solvent B for 10 minutes.

Chromatography:

Article Title: KSHV oral shedding and plasma viremia result in significant changes in the extracellular tumorigenic miRNA expression profile in individuals infected with the malaria parasite
Article Snippet: .. The pH was adjusted to less than 3 with 2% TFA right before desalting using Oasis HLB 10mg cartridge (Waters, Milford, MA) and stored at -20°C until fractionation by strong cation-exchange (SCX) chromatography. .. The pooled sample was dissolved in buffer A (7 mM potassium phosphate, 30% acetonitrile, pH 2.65) immediately before fractionation with a SCX column (PolySulfoethy A, 5 μm, 2.1 × 100 mm, PolyLC Inc., Columbia, MD) The fractions were collected every minute at a flow rate of 200 μL/min from 1% solvent B (7 mM potassium phosphate, 500 mM KCl, 30% acetonitrile, pH 2.65) to 60% over 40 minutes (1% B for 7 minutes, 6–15% B for 18 minutes, 15–34% B for 10 minutes, and 34–60% B for 5 minutes) as well as during column washing with 98% solvent B for 10 minutes.

Article Title: Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells
Article Snippet: .. After allyl deprotection with palladium and purification by silica gel chromatography, the Fmoc-Ser(DMNB)-OH was further purified over a cartridge (Sep-Pak C18; Waters) using a reversed-phase solvent system: 0.1% aqueous trifluoroacetic acid (solvent A) versus 90% acetonitrile plus 0.1% trifluoroacetic acid (solvent B). .. Purified Fmoc-Ser(DMNB)-OH was validated by electrospray mass spectrometry and nuclear magnetic resonance, and it was then incorporated into a modified importin-α peptide at the p8 position by solid-phase peptide synthesis.

Immunoprecipitation:

Article Title: GPR158/179 regulate G protein signaling by controlling localization and activity of the RGS7 complexes
Article Snippet: Paragraph title: Preparative immunoprecipitation and mass spectrometry ... Proteins were digested with 20 µg of modified porcine trypsin (Promega) in the presence of 3 mM CaCl2 at 37°C for 16 h. Peptide mixtures were vacuum dried, reconstituted in 0.2% formic acid (Thermo Fisher Scientific), and applied to a cartridge (Oasis MCX; Waters) preequilibrated with methanol/water (1:1 vol/vol).

Protease Inhibitor:

Article Title: GPR158/179 regulate G protein signaling by controlling localization and activity of the RGS7 complexes
Article Snippet: Preparative immunoprecipitation and mass spectrometry Whole brains were removed from mice, homogenized in immunoprecipitation buffer (PBS, 0.3 mM NaCl, 1% Triton X-100, and protease inhibitor cocktail [Roche]) with a series of needles with increasing gauges, and lysed for 30 min at 4°C. .. Proteins were digested with 20 µg of modified porcine trypsin (Promega) in the presence of 3 mM CaCl2 at 37°C for 16 h. Peptide mixtures were vacuum dried, reconstituted in 0.2% formic acid (Thermo Fisher Scientific), and applied to a cartridge (Oasis MCX; Waters) preequilibrated with methanol/water (1:1 vol/vol).

Generated:

Article Title: EPSIN 3, A Novel p53 Target, Regulates the Apoptotic Pathway and Gastric Carcinogenesis
Article Snippet: Trypsin GOLD solution was added at a ratio of 1/50 (w/w) enzyme to protein and incubated at 37°C for 16 h. The resulting peptides were desalted with an Oasis HLB 1 ml cartridge (Waters) and dried with a vacuum spin dryer. .. Following 4 washes with PBS, N-glycosylated peptides were specifically eluted by incubation with 1 unit of PNGase-F solution (Sigma) at 37°C for 6 h. The released de-glycosylated peptides were finally analyzed with an LTQ-Orbitrap-Velos LC/MS system and subjected to database search analysis in which amino acid substitution (Asn > Asp) was added to the dynamic modification setting in Proteome Discoverer software to determine de-glycosylation sites generated by PNGase-F.

Sequencing:

Article Title: Proteomics and Phosphoproteomics Analysis of Human Lens Fiber Cell Membranes
Article Snippet: The remaining pellets were suspended in 100 μL 10 mM of HCl and 2 μg of sequencing-grade pepsin (Princeton Separation) was added and the sample was incubated at 37°C for 6 hours. .. The ACN extracts and the initially collected supernatants were pooled together and diluted 5 times by 0.1% formic acid and loaded onto a cartridge (SepPak C18; Waters, Milford, MA).

Sonication:

Article Title: Early Responses to Severe Drought Stress in the Arabidopsis thaliana Cell Suspension Culture Proteome
Article Snippet: The samples were then centrifuged at 16,000 g , supernatant was decanted, and pallets were re-suspended in 100 mM HEPES (pH 8.5) and sonicated. .. In a final step, the pooled TMT labelled peptides were desalted using Sep-Pak Vac tC18 100 mg cartridge (Waters, Milford, MA, USA).

Isolation:

Article Title: GPR158/179 regulate G protein signaling by controlling localization and activity of the RGS7 complexes
Article Snippet: Proteins were digested with 20 µg of modified porcine trypsin (Promega) in the presence of 3 mM CaCl2 at 37°C for 16 h. Peptide mixtures were vacuum dried, reconstituted in 0.2% formic acid (Thermo Fisher Scientific), and applied to a cartridge (Oasis MCX; Waters) preequilibrated with methanol/water (1:1 vol/vol). .. Full scans were performed with the orbital trap over a range of mass/charge 360–1,800 at 60,000 resolution using a target value of 106 ions or 500 ms. Tandem mass spectra were obtained using the linear ion trap using collision-induced dissociation with a normalized collision energy of 35%, an isolation width of 2.0 mass/charge, and target values of 10,000 ions or 100 ms.

Transfection:

Article Title: Probing the Production of Amidated Peptides following Genetic and Dietary Copper Manipulations
Article Snippet: Synthetic ACTH(1-13)-Gly [ACTH(1-14)] (AnaSpec, Fremont, CA, USA) was converted into ACTH(1-13)-Gly-OH using PHM catalytic core purified from stably transfected Chinese hamster ovary cell medium , , . .. The peptide was adsorbed to a SepPak C18 μBondapak cartridge (Waters Corporation, Milford, MA, USA), which was then washed with 0.1% TFA and eluted with 80% ACN in 0.1% TFA.

Article Title: EPSIN 3, A Novel p53 Target, Regulates the Apoptotic Pathway and Gastric Carcinogenesis
Article Snippet: Quantification of Membrane Proteins by Mass Spectrometric Analysis The siEPN3-b or siEPN3-d vector was transfected into HCT116 cells. .. Trypsin GOLD solution was added at a ratio of 1/50 (w/w) enzyme to protein and incubated at 37°C for 16 h. The resulting peptides were desalted with an Oasis HLB 1 ml cartridge (Waters) and dried with a vacuum spin dryer.

Labeling:

Article Title: KSHV oral shedding and plasma viremia result in significant changes in the extracellular tumorigenic miRNA expression profile in individuals infected with the malaria parasite
Article Snippet: The labeled peptides from 10 samples were dissolved in 50 μL 0.1% TFA in each vial and combined. .. The pH was adjusted to less than 3 with 2% TFA right before desalting using Oasis HLB 10mg cartridge (Waters, Milford, MA) and stored at -20°C until fractionation by strong cation-exchange (SCX) chromatography.

Purification:

Article Title: Probing the Production of Amidated Peptides following Genetic and Dietary Copper Manipulations
Article Snippet: Synthetic ACTH(1-13)-Gly [ACTH(1-14)] (AnaSpec, Fremont, CA, USA) was converted into ACTH(1-13)-Gly-OH using PHM catalytic core purified from stably transfected Chinese hamster ovary cell medium , , . .. The peptide was adsorbed to a SepPak C18 μBondapak cartridge (Waters Corporation, Milford, MA, USA), which was then washed with 0.1% TFA and eluted with 80% ACN in 0.1% TFA.

Article Title: Ultra-Performance Liquid Chromatography and Time-of-Flight Mass Spectrometry Analysis of Ginsenoside Metabolites in Human Plasma
Article Snippet: .. The homogenate was purified by solid-phase extraction (SPE) with a Sep-Pak C8 Vac 3cc 500 mg cartridge (Waters, Milford, MA). ..

Article Title: Anti-inflammatory Activity and Phytochemical Profile of Galinsoga Parviflora Cav.
Article Snippet: The cartridge (Oasis HLB Plus Short Cartridge, Waters, Milford, MA, USA) was conditioned with 5 mL of methanol followed by 5 mL of grade water. .. The organic eluate was collected in a flask and then concentrated under the vacuum at 40°C to dryness, diluted with mixture methanol-water (1:1, v /v ) to 2.0 mL, and then purified through a syringe filter (GHP, 25 mm, 0.2 µm, Waters).

Article Title: Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells
Article Snippet: .. After allyl deprotection with palladium and purification by silica gel chromatography, the Fmoc-Ser(DMNB)-OH was further purified over a cartridge (Sep-Pak C18; Waters) using a reversed-phase solvent system: 0.1% aqueous trifluoroacetic acid (solvent A) versus 90% acetonitrile plus 0.1% trifluoroacetic acid (solvent B). .. Purified Fmoc-Ser(DMNB)-OH was validated by electrospray mass spectrometry and nuclear magnetic resonance, and it was then incorporated into a modified importin-α peptide at the p8 position by solid-phase peptide synthesis.

Mouse Assay:

Article Title: GPR158/179 regulate G protein signaling by controlling localization and activity of the RGS7 complexes
Article Snippet: Preparative immunoprecipitation and mass spectrometry Whole brains were removed from mice, homogenized in immunoprecipitation buffer (PBS, 0.3 mM NaCl, 1% Triton X-100, and protease inhibitor cocktail [Roche]) with a series of needles with increasing gauges, and lysed for 30 min at 4°C. .. Proteins were digested with 20 µg of modified porcine trypsin (Promega) in the presence of 3 mM CaCl2 at 37°C for 16 h. Peptide mixtures were vacuum dried, reconstituted in 0.2% formic acid (Thermo Fisher Scientific), and applied to a cartridge (Oasis MCX; Waters) preequilibrated with methanol/water (1:1 vol/vol).

Liquid Chromatography:

Article Title: GPR158/179 regulate G protein signaling by controlling localization and activity of the RGS7 complexes
Article Snippet: Proteins were digested with 20 µg of modified porcine trypsin (Promega) in the presence of 3 mM CaCl2 at 37°C for 16 h. Peptide mixtures were vacuum dried, reconstituted in 0.2% formic acid (Thermo Fisher Scientific), and applied to a cartridge (Oasis MCX; Waters) preequilibrated with methanol/water (1:1 vol/vol). .. The resulting peptide mixtures were desalted, resolved by liquid chromatography, and analyzed by mass spectrometry (LTQ Orbitrap XL; Thermo Fisher Scientific) as previously described ( ).

Plasmid Preparation:

Article Title: EPSIN 3, A Novel p53 Target, Regulates the Apoptotic Pathway and Gastric Carcinogenesis
Article Snippet: Quantification of Membrane Proteins by Mass Spectrometric Analysis The siEPN3-b or siEPN3-d vector was transfected into HCT116 cells. .. Trypsin GOLD solution was added at a ratio of 1/50 (w/w) enzyme to protein and incubated at 37°C for 16 h. The resulting peptides were desalted with an Oasis HLB 1 ml cartridge (Waters) and dried with a vacuum spin dryer.

Software:

Article Title: EPSIN 3, A Novel p53 Target, Regulates the Apoptotic Pathway and Gastric Carcinogenesis
Article Snippet: Trypsin GOLD solution was added at a ratio of 1/50 (w/w) enzyme to protein and incubated at 37°C for 16 h. The resulting peptides were desalted with an Oasis HLB 1 ml cartridge (Waters) and dried with a vacuum spin dryer. .. Following 4 washes with PBS, N-glycosylated peptides were specifically eluted by incubation with 1 unit of PNGase-F solution (Sigma) at 37°C for 6 h. The released de-glycosylated peptides were finally analyzed with an LTQ-Orbitrap-Velos LC/MS system and subjected to database search analysis in which amino acid substitution (Asn > Asp) was added to the dynamic modification setting in Proteome Discoverer software to determine de-glycosylation sites generated by PNGase-F.

Article Title: A Comparative Proteomic Analysis of the KATP channel Complex in Different Tissue Types
Article Snippet: For all LC-MSE experiments, a QTof-Premier (Waters) coupled to a nanoAcquity Ultra Performance LC (Waters) with a 5 µm Acclaim PepMap100 C18 µ-precolumn cartridge (300 µm × 5 mm; LC Packings) and a 1.7 µm BEH130 C18 analytical column (100 µm × 100 µm; Waters). .. Data acquired via LC-MSE were processed using ProteinLynx Global Server (PLGS) software (version 2.4, Waters).

Hydrophilic Interaction Liquid Chromatography:

Article Title: Comparison of Enrichment Methods for Intact N- and O-Linked Glycopeptides Using Strong Anion Exchange and Hydrophilic Interaction Liquid Chromatography
Article Snippet: After C18 desalting, peptides eluted in 60% ACN (v/v), 0.1% TFA (v/v) were adjusted to 80% ACN (v/v), 1% TFA (v/v) for glycopeptide enrichment using ZIC-HILIC (The Nest Group, Inc. or 95% ACN (v/v), 1% TFA (v/v) or using SAX (HyperSep Retain AX Cartridges (RAX), particle size 30-50 μ m, 30 mg sorbent per cartridge (Thermo Fisher Scientific) and Oasis MAX Cartridge particle size 30 μ m, 30 mg sorbent per cartridge (Waters)). .. For HILIC, cartridges were conditioned in water for three times followed by 1 mL of 80% ACN (v/v), 1% TFA (v/v) for three times, the samples were then loaded, washed for three times in 1 mL of 80% ACN (v/v), 1% TFA (v/v) and eluted in 400 μ L of 0.1% TFA.

Sample Prep:

Article Title: KSHV oral shedding and plasma viremia result in significant changes in the extracellular tumorigenic miRNA expression profile in individuals infected with the malaria parasite
Article Snippet: Paragraph title: Sample preparation and protein fractionation ... The pH was adjusted to less than 3 with 2% TFA right before desalting using Oasis HLB 10mg cartridge (Waters, Milford, MA) and stored at -20°C until fractionation by strong cation-exchange (SCX) chromatography.

Article Title: Ultra-Performance Liquid Chromatography and Time-of-Flight Mass Spectrometry Analysis of Ginsenoside Metabolites in Human Plasma
Article Snippet: Paragraph title: Sample Preparation and Analysis ... The homogenate was purified by solid-phase extraction (SPE) with a Sep-Pak C8 Vac 3cc 500 mg cartridge (Waters, Milford, MA).

Article Title: Anti-inflammatory Activity and Phytochemical Profile of Galinsoga Parviflora Cav.
Article Snippet: Paragraph title: Sample Preparation ... The cartridge (Oasis HLB Plus Short Cartridge, Waters, Milford, MA, USA) was conditioned with 5 mL of methanol followed by 5 mL of grade water.

Nuclear Magnetic Resonance:

Article Title: Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells
Article Snippet: After allyl deprotection with palladium and purification by silica gel chromatography, the Fmoc-Ser(DMNB)-OH was further purified over a cartridge (Sep-Pak C18; Waters) using a reversed-phase solvent system: 0.1% aqueous trifluoroacetic acid (solvent A) versus 90% acetonitrile plus 0.1% trifluoroacetic acid (solvent B). .. Purified Fmoc-Ser(DMNB)-OH was validated by electrospray mass spectrometry and nuclear magnetic resonance, and it was then incorporated into a modified importin-α peptide at the p8 position by solid-phase peptide synthesis.

Concentration Assay:

Article Title: Proteomics and Phosphoproteomics Analysis of Human Lens Fiber Cell Membranes
Article Snippet: The ACN extracts were dried by vacuum concentration and reconstituted in 20 μL 5% ACN, 0.1% formic acid. .. The ACN extracts and the initially collected supernatants were pooled together and diluted 5 times by 0.1% formic acid and loaded onto a cartridge (SepPak C18; Waters, Milford, MA).

Fractionation:

Article Title: KSHV oral shedding and plasma viremia result in significant changes in the extracellular tumorigenic miRNA expression profile in individuals infected with the malaria parasite
Article Snippet: .. The pH was adjusted to less than 3 with 2% TFA right before desalting using Oasis HLB 10mg cartridge (Waters, Milford, MA) and stored at -20°C until fractionation by strong cation-exchange (SCX) chromatography. .. The pooled sample was dissolved in buffer A (7 mM potassium phosphate, 30% acetonitrile, pH 2.65) immediately before fractionation with a SCX column (PolySulfoethy A, 5 μm, 2.1 × 100 mm, PolyLC Inc., Columbia, MD) The fractions were collected every minute at a flow rate of 200 μL/min from 1% solvent B (7 mM potassium phosphate, 500 mM KCl, 30% acetonitrile, pH 2.65) to 60% over 40 minutes (1% B for 7 minutes, 6–15% B for 18 minutes, 15–34% B for 10 minutes, and 34–60% B for 5 minutes) as well as during column washing with 98% solvent B for 10 minutes.

Liquid Chromatography with Mass Spectroscopy:

Article Title: An assessment of the concentrations of pharmaceutical compounds in wastewater treatment plants on the island of Gran Canaria (Spain)
Article Snippet: For Groups 1 and 2, the SPE cartridge used was an OASIS HLB 6 mL/200 mg cartridge (Waters, Spain). .. The eluents were then evaporated under a gentle nitrogen stream and reconstituted with 1 ± 0.01 mL of LC-MS grade water.

Article Title: EPSIN 3, A Novel p53 Target, Regulates the Apoptotic Pathway and Gastric Carcinogenesis
Article Snippet: Trypsin GOLD solution was added at a ratio of 1/50 (w/w) enzyme to protein and incubated at 37°C for 16 h. The resulting peptides were desalted with an Oasis HLB 1 ml cartridge (Waters) and dried with a vacuum spin dryer. .. Following 4 washes with PBS, N-glycosylated peptides were specifically eluted by incubation with 1 unit of PNGase-F solution (Sigma) at 37°C for 6 h. The released de-glycosylated peptides were finally analyzed with an LTQ-Orbitrap-Velos LC/MS system and subjected to database search analysis in which amino acid substitution (Asn > Asp) was added to the dynamic modification setting in Proteome Discoverer software to determine de-glycosylation sites generated by PNGase-F.

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    Waters Corporation 100 mm silica cartridge
    Opposing effects of lipin 1 and lipin 2 depletion in lipid droplet biogenesis of 3T3-L1 cells. A , 3T3-L1 adipocytes were transfected with nontargeting (control, ctr ) or lipin 1 and lipin 2 ( L1 + 2 ) siRNA at days 4 and 6 after induction of differentiation. Day 8 adipocytes were fixed, stained with BODIPY 493/503, and imaged with an LSM 710 confocal microscope. Three-dimensional reconstruction from Z-stacks to visualize lipid droplets was performed as described under “Experimental Procedures.” Representative three-dimensional reconstructions from control or L1 + 2 adipocytes are shown. Rectangle side size was <t>100</t> μm. B , scatter plot of lipid droplet number ( x axis ) versus average lipid droplet volume ( y axis) per cell in control or L1 + 2 3T3-L1 day 8 cells. Representative data from three separate experiments are shown. C , distribution of lipid droplet numbers in 3T3-L1 day 8 cells transfected with nontargeting (control, ctr ), lipin 1 and lipin 2 ( L1 + 2 ), lipin 1 only ( L1 ), or lipin 2 only ( L2 ) siRNA at days 4 and 6. D , average total lipid droplet volumes per cell for the experiments shown in C . The values are normalized to the lipid droplet volumes per cell of the control ( ctr ) 3T3-L1 transfectants, set at 1. The experiments in C and D represent means ± S.D. of at least three (control and L1 + 2) or two (L1 and L2) experiments. The total number of cells analyzed: control, 423; L1 + 2, 334; L1 203; and L2, 235. *, p
    100 Mm Silica Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/100 mm silica cartridge/product/Waters Corporation
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    100 mm silica cartridge - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    93
    Waters Corporation tc2 sep pak cartridge
    Analysis of 99m Tc-HYNIC-IL2 stability in human serum, saline, and DTPA solution after purification by <t>tC2</t> <t>Sep-Pak.</t>
    Tc2 Sep Pak Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tc2 sep pak cartridge/product/Waters Corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tc2 sep pak cartridge - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    94
    Waters Corporation c18 cartridges
    Selection of iRT-peptides and definition of <t>iRT-C18</t> scale
    C18 Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 cartridges/product/Waters Corporation
    Average 94 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    c18 cartridges - by Bioz Stars, 2020-04
    94/100 stars
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    97
    Waters Corporation sep pak light c18 cartridge
    (a) Purification of 89 Zr-DFO-LEM2/15 in a PD-10 column; the antibody elutes between volume fractions 2.5 and 5.0 ml. Inset: ITLC image illustrating RQP of the 89 Zr-DFO-LEM2/15 peak. 1 corresponds to the radioimmunoconjugate and 2 to the free radionuclide. (b) Percentage of bound radioactivity to LEM2/15 after 7-day incubation of 89 Zr-DFO-LEM2/15 with human serum at 37°C ( ) and 4°C ( ) and human plasma at 37°C ( ) and 4°C ( ); data expressed as mean ± SD. ( n =4). Representative HPLC chromatogram of 68 Ga-DOTA-AF7p-1 before (c) or after (d) to <t>Sep-Pak</t> Light <t>C18</t> cartridge purification. 68 Ga-DOTA-AF7p-1 is detected at 8.7 min by UV absorbance at 280 nm ( ) and radioactivity detector ( ).
    Sep Pak Light C18 Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 97/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sep pak light c18 cartridge/product/Waters Corporation
    Average 97 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    sep pak light c18 cartridge - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

    Image Search Results


    Opposing effects of lipin 1 and lipin 2 depletion in lipid droplet biogenesis of 3T3-L1 cells. A , 3T3-L1 adipocytes were transfected with nontargeting (control, ctr ) or lipin 1 and lipin 2 ( L1 + 2 ) siRNA at days 4 and 6 after induction of differentiation. Day 8 adipocytes were fixed, stained with BODIPY 493/503, and imaged with an LSM 710 confocal microscope. Three-dimensional reconstruction from Z-stacks to visualize lipid droplets was performed as described under “Experimental Procedures.” Representative three-dimensional reconstructions from control or L1 + 2 adipocytes are shown. Rectangle side size was 100 μm. B , scatter plot of lipid droplet number ( x axis ) versus average lipid droplet volume ( y axis) per cell in control or L1 + 2 3T3-L1 day 8 cells. Representative data from three separate experiments are shown. C , distribution of lipid droplet numbers in 3T3-L1 day 8 cells transfected with nontargeting (control, ctr ), lipin 1 and lipin 2 ( L1 + 2 ), lipin 1 only ( L1 ), or lipin 2 only ( L2 ) siRNA at days 4 and 6. D , average total lipid droplet volumes per cell for the experiments shown in C . The values are normalized to the lipid droplet volumes per cell of the control ( ctr ) 3T3-L1 transfectants, set at 1. The experiments in C and D represent means ± S.D. of at least three (control and L1 + 2) or two (L1 and L2) experiments. The total number of cells analyzed: control, 423; L1 + 2, 334; L1 203; and L2, 235. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Distinct Roles of the Phosphatidate Phosphatases Lipin 1 and 2 during Adipogenesis and Lipid Droplet Biogenesis in 3T3-L1 Cells *

    doi: 10.1074/jbc.M113.488445

    Figure Lengend Snippet: Opposing effects of lipin 1 and lipin 2 depletion in lipid droplet biogenesis of 3T3-L1 cells. A , 3T3-L1 adipocytes were transfected with nontargeting (control, ctr ) or lipin 1 and lipin 2 ( L1 + 2 ) siRNA at days 4 and 6 after induction of differentiation. Day 8 adipocytes were fixed, stained with BODIPY 493/503, and imaged with an LSM 710 confocal microscope. Three-dimensional reconstruction from Z-stacks to visualize lipid droplets was performed as described under “Experimental Procedures.” Representative three-dimensional reconstructions from control or L1 + 2 adipocytes are shown. Rectangle side size was 100 μm. B , scatter plot of lipid droplet number ( x axis ) versus average lipid droplet volume ( y axis) per cell in control or L1 + 2 3T3-L1 day 8 cells. Representative data from three separate experiments are shown. C , distribution of lipid droplet numbers in 3T3-L1 day 8 cells transfected with nontargeting (control, ctr ), lipin 1 and lipin 2 ( L1 + 2 ), lipin 1 only ( L1 ), or lipin 2 only ( L2 ) siRNA at days 4 and 6. D , average total lipid droplet volumes per cell for the experiments shown in C . The values are normalized to the lipid droplet volumes per cell of the control ( ctr ) 3T3-L1 transfectants, set at 1. The experiments in C and D represent means ± S.D. of at least three (control and L1 + 2) or two (L1 and L2) experiments. The total number of cells analyzed: control, 423; L1 + 2, 334; L1 203; and L2, 235. *, p

    Article Snippet: The high performance liquid chromatography column was a Waters SpherisorbS5W 4.6 × 100-mm silica cartridge, 5-μm particle size, with a Waters Spherisorb S5W 4.6 × 10-mm guard cartridge.

    Techniques: Transfection, Staining, Microscopy

    Analysis of 99m Tc-HYNIC-IL2 stability in human serum, saline, and DTPA solution after purification by tC2 Sep-Pak.

    Journal: Molecular Imaging and Biology

    Article Title: Synthesis and Optimization of the Labeling Procedure of 99mTc-Hynic-Interleukin-2 for In vivo Imaging of Activated T lymphocytes

    doi: 10.1007/s11307-009-0285-1

    Figure Lengend Snippet: Analysis of 99m Tc-HYNIC-IL2 stability in human serum, saline, and DTPA solution after purification by tC2 Sep-Pak.

    Article Snippet: The IL2 conjugate purified by tC2 Sep-Pak cartridge and stored in liquid nitrogen showed a good stability for at least 30 days.

    Techniques: Purification

    Selection of iRT-peptides and definition of iRT-C18 scale

    Journal: Proteomics

    Article Title: Using iRT, a normalized retention time for more targeted measurement of peptides

    doi: 10.1002/pmic.201100463

    Figure Lengend Snippet: Selection of iRT-peptides and definition of iRT-C18 scale

    Article Snippet: Peptides were acidified with 1% trifluoroacetic acid (TFA, Thermo Scientific) and purified by solid-phase extraction using C18 cartridges (Sep-Pak, Waters).

    Techniques: Selection

    (a) Purification of 89 Zr-DFO-LEM2/15 in a PD-10 column; the antibody elutes between volume fractions 2.5 and 5.0 ml. Inset: ITLC image illustrating RQP of the 89 Zr-DFO-LEM2/15 peak. 1 corresponds to the radioimmunoconjugate and 2 to the free radionuclide. (b) Percentage of bound radioactivity to LEM2/15 after 7-day incubation of 89 Zr-DFO-LEM2/15 with human serum at 37°C ( ) and 4°C ( ) and human plasma at 37°C ( ) and 4°C ( ); data expressed as mean ± SD. ( n =4). Representative HPLC chromatogram of 68 Ga-DOTA-AF7p-1 before (c) or after (d) to Sep-Pak Light C18 cartridge purification. 68 Ga-DOTA-AF7p-1 is detected at 8.7 min by UV absorbance at 280 nm ( ) and radioactivity detector ( ).

    Journal: Contrast Media & Molecular Imaging

    Article Title: MT1-MMP as a PET Imaging Biomarker for Pancreas Cancer Management

    doi: 10.1155/2018/8382148

    Figure Lengend Snippet: (a) Purification of 89 Zr-DFO-LEM2/15 in a PD-10 column; the antibody elutes between volume fractions 2.5 and 5.0 ml. Inset: ITLC image illustrating RQP of the 89 Zr-DFO-LEM2/15 peak. 1 corresponds to the radioimmunoconjugate and 2 to the free radionuclide. (b) Percentage of bound radioactivity to LEM2/15 after 7-day incubation of 89 Zr-DFO-LEM2/15 with human serum at 37°C ( ) and 4°C ( ) and human plasma at 37°C ( ) and 4°C ( ); data expressed as mean ± SD. ( n =4). Representative HPLC chromatogram of 68 Ga-DOTA-AF7p-1 before (c) or after (d) to Sep-Pak Light C18 cartridge purification. 68 Ga-DOTA-AF7p-1 is detected at 8.7 min by UV absorbance at 280 nm ( ) and radioactivity detector ( ).

    Article Snippet: The radiochromatogram of the reaction mixture prior to passing through the Sep-Pak Light C18 cartridge showed two distinct peaks corresponding to free 68 Ga and 68 Ga-DOTA-AF7p-1 with retention times of 2.5 and 8.7 min, respectively. shows a representative chromatogram of peptide labelling with a low yield aiming at demonstrating that even in those cases, a final product can be successfully obtained.

    Techniques: Purification, Radioactivity, Incubation, High Performance Liquid Chromatography