capita cytomegalovirus igg enzyme linked immunosorbent assay elisa kit  (Trinity Biotech)

 
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    Trinity Biotech capita cytomegalovirus igg enzyme linked immunosorbent assay elisa kit
    Alveolar macrophages from seropositive individuals express immediate early gene (IE) messenger RNA (mRNA). A , Serum specimens from 10 donor patients (P) were tested for the presence of IE immunoglobulin G <t>(IgG)</t> by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA).</t> B , Alveolar macrophages were isolated directly ex vivo from the same patients and harvested for reverse-transcription quantitative polymerase chain reaction (PCR) analysis for the presence of human <t>cytomegalovirus</t> (HCMV) IE mRNA. A blood specimen from each patient was also assessed by PCR for the presence of HCMV DNA, to determine whether viremia was present, and for the presence of IgM. These data and the data from panels A and B are summarized in Table 1 .
    Capita Cytomegalovirus Igg Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/capita cytomegalovirus igg enzyme linked immunosorbent assay elisa kit/product/Trinity Biotech
    Average 89 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    capita cytomegalovirus igg enzyme linked immunosorbent assay elisa kit - by Bioz Stars, 2020-07
    89/100 stars

    Related Products / Commonly Used Together

    hcmv immunoglobulin g igg serotyping
    donors hcmv-specific igg

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    1) Product Images from "Alveolar Macrophages Isolated Directly From Human Cytomegalovirus (HCMV)–Seropositive Individuals Are Sites of HCMV Reactivation In Vivo"

    Article Title: Alveolar Macrophages Isolated Directly From Human Cytomegalovirus (HCMV)–Seropositive Individuals Are Sites of HCMV Reactivation In Vivo

    Journal: The Journal of Infectious Diseases

    doi: 10.1093/infdis/jiu837

    Alveolar macrophages from seropositive individuals express immediate early gene (IE) messenger RNA (mRNA). A , Serum specimens from 10 donor patients (P) were tested for the presence of IE immunoglobulin G (IgG) by enzyme-linked immunosorbent assay (ELISA). B , Alveolar macrophages were isolated directly ex vivo from the same patients and harvested for reverse-transcription quantitative polymerase chain reaction (PCR) analysis for the presence of human cytomegalovirus (HCMV) IE mRNA. A blood specimen from each patient was also assessed by PCR for the presence of HCMV DNA, to determine whether viremia was present, and for the presence of IgM. These data and the data from panels A and B are summarized in Table 1 .
    Figure Legend Snippet: Alveolar macrophages from seropositive individuals express immediate early gene (IE) messenger RNA (mRNA). A , Serum specimens from 10 donor patients (P) were tested for the presence of IE immunoglobulin G (IgG) by enzyme-linked immunosorbent assay (ELISA). B , Alveolar macrophages were isolated directly ex vivo from the same patients and harvested for reverse-transcription quantitative polymerase chain reaction (PCR) analysis for the presence of human cytomegalovirus (HCMV) IE mRNA. A blood specimen from each patient was also assessed by PCR for the presence of HCMV DNA, to determine whether viremia was present, and for the presence of IgM. These data and the data from panels A and B are summarized in Table 1 .

    Techniques Used: Enzyme-linked Immunosorbent Assay, Isolation, Ex Vivo, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

    Monocytes ex vivo from a human cytomegalovirus (HCMV)-seropositive patient transcribe the HCMV latency gene UL138, and alveolar macrophages from the same donor express the HCMV lytic immediate early gene (IE) protein. Donors were tested for systemic inflammation (as revealed by the presence of C-reactive protein) and local bronchoalveolar fluid (BALF) inflammation (by means of enzyme-linked immunosorbent assay [ELISA] specific for tumor necrosis factor α [TNF-α]). A positive control for the ELISA was supernatant from monocyte-derived dendritic cells (DCs) shown in Table 2 . A , Monocytes were isolated from venous blood and harvested for reverse-transcription quantitative polymerase chain reaction analysis. B , Isolated alveolar macrophages were cytospun and then fixed and stained for detection of the HCMV lytic antigen, IE. Cells were counterstained with Hoechst stain to detect nuclei.
    Figure Legend Snippet: Monocytes ex vivo from a human cytomegalovirus (HCMV)-seropositive patient transcribe the HCMV latency gene UL138, and alveolar macrophages from the same donor express the HCMV lytic immediate early gene (IE) protein. Donors were tested for systemic inflammation (as revealed by the presence of C-reactive protein) and local bronchoalveolar fluid (BALF) inflammation (by means of enzyme-linked immunosorbent assay [ELISA] specific for tumor necrosis factor α [TNF-α]). A positive control for the ELISA was supernatant from monocyte-derived dendritic cells (DCs) shown in Table 2 . A , Monocytes were isolated from venous blood and harvested for reverse-transcription quantitative polymerase chain reaction analysis. B , Isolated alveolar macrophages were cytospun and then fixed and stained for detection of the HCMV lytic antigen, IE. Cells were counterstained with Hoechst stain to detect nuclei.

    Techniques Used: Ex Vivo, Enzyme-linked Immunosorbent Assay, Positive Control, Derivative Assay, Isolation, Real-time Polymerase Chain Reaction, Staining

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Alveolar Macrophages Isolated Directly From Human Cytomegalovirus (HCMV)–Seropositive Individuals Are Sites of HCMV Reactivation In Vivo
    Article Snippet: .. HCMV Immunoglobulin G (IgG) Serotyping of Donors HCMV-specific IgG was assessed in patients’ serum specimens by the Capita Cytomegalovirus IgG enzyme-linked immunosorbent assay (ELISA) kit (Trinity Biotech) according to the manufacturer's instructions. .. HCMV Immunoglobulin M (IgM) Serotyping of Donors Donor serum specimens were tested using the Liaison CMV IgM II chemiluminescence immunoassay (DiaSorin) for the semiquantitative determination of specific IgM antibodies to HCMV in human serum or plasma samples, according to the manufacturer's protocol.

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    Trinity Biotech capita cytomegalovirus igg enzyme linked immunosorbent assay elisa kit
    Alveolar macrophages from seropositive individuals express immediate early gene (IE) messenger RNA (mRNA). A , Serum specimens from 10 donor patients (P) were tested for the presence of IE immunoglobulin G <t>(IgG)</t> by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA).</t> B , Alveolar macrophages were isolated directly ex vivo from the same patients and harvested for reverse-transcription quantitative polymerase chain reaction (PCR) analysis for the presence of human <t>cytomegalovirus</t> (HCMV) IE mRNA. A blood specimen from each patient was also assessed by PCR for the presence of HCMV DNA, to determine whether viremia was present, and for the presence of IgM. These data and the data from panels A and B are summarized in Table 1 .
    Capita Cytomegalovirus Igg Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/capita cytomegalovirus igg enzyme linked immunosorbent assay elisa kit/product/Trinity Biotech
    Average 89 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    capita cytomegalovirus igg enzyme linked immunosorbent assay elisa kit - by Bioz Stars, 2020-07
    89/100 stars
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    Alveolar macrophages from seropositive individuals express immediate early gene (IE) messenger RNA (mRNA). A , Serum specimens from 10 donor patients (P) were tested for the presence of IE immunoglobulin G (IgG) by enzyme-linked immunosorbent assay (ELISA). B , Alveolar macrophages were isolated directly ex vivo from the same patients and harvested for reverse-transcription quantitative polymerase chain reaction (PCR) analysis for the presence of human cytomegalovirus (HCMV) IE mRNA. A blood specimen from each patient was also assessed by PCR for the presence of HCMV DNA, to determine whether viremia was present, and for the presence of IgM. These data and the data from panels A and B are summarized in Table 1 .

    Journal: The Journal of Infectious Diseases

    Article Title: Alveolar Macrophages Isolated Directly From Human Cytomegalovirus (HCMV)–Seropositive Individuals Are Sites of HCMV Reactivation In Vivo

    doi: 10.1093/infdis/jiu837

    Figure Lengend Snippet: Alveolar macrophages from seropositive individuals express immediate early gene (IE) messenger RNA (mRNA). A , Serum specimens from 10 donor patients (P) were tested for the presence of IE immunoglobulin G (IgG) by enzyme-linked immunosorbent assay (ELISA). B , Alveolar macrophages were isolated directly ex vivo from the same patients and harvested for reverse-transcription quantitative polymerase chain reaction (PCR) analysis for the presence of human cytomegalovirus (HCMV) IE mRNA. A blood specimen from each patient was also assessed by PCR for the presence of HCMV DNA, to determine whether viremia was present, and for the presence of IgM. These data and the data from panels A and B are summarized in Table 1 .

    Article Snippet: HCMV Immunoglobulin G (IgG) Serotyping of Donors HCMV-specific IgG was assessed in patients’ serum specimens by the Capita Cytomegalovirus IgG enzyme-linked immunosorbent assay (ELISA) kit (Trinity Biotech) according to the manufacturer's instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Isolation, Ex Vivo, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

    Monocytes ex vivo from a human cytomegalovirus (HCMV)-seropositive patient transcribe the HCMV latency gene UL138, and alveolar macrophages from the same donor express the HCMV lytic immediate early gene (IE) protein. Donors were tested for systemic inflammation (as revealed by the presence of C-reactive protein) and local bronchoalveolar fluid (BALF) inflammation (by means of enzyme-linked immunosorbent assay [ELISA] specific for tumor necrosis factor α [TNF-α]). A positive control for the ELISA was supernatant from monocyte-derived dendritic cells (DCs) shown in Table 2 . A , Monocytes were isolated from venous blood and harvested for reverse-transcription quantitative polymerase chain reaction analysis. B , Isolated alveolar macrophages were cytospun and then fixed and stained for detection of the HCMV lytic antigen, IE. Cells were counterstained with Hoechst stain to detect nuclei.

    Journal: The Journal of Infectious Diseases

    Article Title: Alveolar Macrophages Isolated Directly From Human Cytomegalovirus (HCMV)–Seropositive Individuals Are Sites of HCMV Reactivation In Vivo

    doi: 10.1093/infdis/jiu837

    Figure Lengend Snippet: Monocytes ex vivo from a human cytomegalovirus (HCMV)-seropositive patient transcribe the HCMV latency gene UL138, and alveolar macrophages from the same donor express the HCMV lytic immediate early gene (IE) protein. Donors were tested for systemic inflammation (as revealed by the presence of C-reactive protein) and local bronchoalveolar fluid (BALF) inflammation (by means of enzyme-linked immunosorbent assay [ELISA] specific for tumor necrosis factor α [TNF-α]). A positive control for the ELISA was supernatant from monocyte-derived dendritic cells (DCs) shown in Table 2 . A , Monocytes were isolated from venous blood and harvested for reverse-transcription quantitative polymerase chain reaction analysis. B , Isolated alveolar macrophages were cytospun and then fixed and stained for detection of the HCMV lytic antigen, IE. Cells were counterstained with Hoechst stain to detect nuclei.

    Article Snippet: HCMV Immunoglobulin G (IgG) Serotyping of Donors HCMV-specific IgG was assessed in patients’ serum specimens by the Capita Cytomegalovirus IgG enzyme-linked immunosorbent assay (ELISA) kit (Trinity Biotech) according to the manufacturer's instructions.

    Techniques: Ex Vivo, Enzyme-linked Immunosorbent Assay, Positive Control, Derivative Assay, Isolation, Real-time Polymerase Chain Reaction, Staining