Structured Review

Thermo Fisher canx
Canx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/canx/product/Thermo Fisher
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
canx - by Bioz Stars, 2020-10
88/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Acetylation of p65 at lysine 314 is important for late NF-κB-dependent gene expression
Article Snippet: .. Real-time PCR was performed using the Rotor-Gene 3000 (Corbett Life Science, now Qiagen) and TaqMan assays from Applied Biosystems for the following genes: Cfb, Mpa2l, Mmp10, Mmp13, Rps6 and CanX , according to Applied Biosystems' protocol. ..

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  • 94
    Thermo Fisher anti cnx
    CD8 TMD* is integrated into the membrane with the correct topology. (A) Cartoon of CD8 chimeras used. (B) Stable HeLa cell lines expressing CD8 WT or CD8 TMD* were fixed and labelled for total (permeabilised cells, anti-HA antibodies) and cell surface (intact cells, anti-CD8 antibodies) CD8. Images were collected in parallel using equal exposure times. (C) Cells expressing CD8 TMD* were lysed and subjected to carbonate extraction. Equivalent amounts of supernatant 1 (initial lysate supernatant), 2 and 3 (from sequential carbonate extractions) and the final membrane pellet were analysed by <t>immunoblotting</t> (IB) with antibodies against HA, calnexin <t>(CNX),</t> calrecticulin (CRT) and Hsp70. (D) Lysates of cells expressing CD8 TMD* or CD8 TMD*VN were treated with or without EndoH and analysed by immunoblotting with antibodies against HA, STT3B and actin. Closed arrowhead indicates N-glycosylated protein, open arrowhead indicates deglycosylated protein. (E) Cells expressing CD8 TMD* were permeabilised with digitonin or Triton-X 100 and labelled with antibodies against the extracellular (luminal) domain of CD8 and the cytoplasmic HA epitope. DNA was stained with DAPI. Scale bars: 10 µm.
    Anti Cnx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cnx/product/Thermo Fisher
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti cnx - by Bioz Stars, 2020-10
    94/100 stars
      Buy from Supplier

    85
    Thermo Fisher full length human calnexin canx coding sequence
    Association of erlins with the SREBP regulatory machinery. (A) CoIP of SREBP pathway proteins with endogenous erlin-2 in lysates of HeLa cells grown with complete (F) or LD medium. Samples were probed by Western blotting as indicated. <t>CANX,</t> <t>calnexin.</t> 10 times more cell equivalents were loaded in IP lanes compared with input, except for erlin-2 blot (1 equivalent). Western blot images are typical of three separate repeats. Numbers below the panel indicate LD/F ratios of IP target proteins averaged from three experiments. P-values for LD versus complete (F): Scap, Srebp-1, and Srebp-2,
    Full Length Human Calnexin Canx Coding Sequence, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/full length human calnexin canx coding sequence/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    full length human calnexin canx coding sequence - by Bioz Stars, 2020-10
    85/100 stars
      Buy from Supplier

    85
    Thermo Fisher sirna transfection cnx depletion
    <t>CNX</t> knockdown partially inhibits CFTR folding. (A) Selective down-regulation of CNX with 100 nM <t>siRNA</t> in CFTR-expressing HeLa cells. Equal amounts of nonspecific (n.s.) and CNX siRNA–treated lysates were analyzed by immunoblotting. C and B indicate complex-glycosylated CFTR and core-glycosylated CFTR, respectively. (B) Folding yield of wt CFTR in CNX-depleted HeLa cells was measured by pulse-chase experiments ( n = 3). (C) Effect of CNX knockdown on the cell surface density of CFTR in HeLa cells ( n = 3). Error bars indicate mean ± SEM.
    Sirna Transfection Cnx Depletion, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sirna transfection cnx depletion/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sirna transfection cnx depletion - by Bioz Stars, 2020-10
    85/100 stars
      Buy from Supplier

    Image Search Results


    CD8 TMD* is integrated into the membrane with the correct topology. (A) Cartoon of CD8 chimeras used. (B) Stable HeLa cell lines expressing CD8 WT or CD8 TMD* were fixed and labelled for total (permeabilised cells, anti-HA antibodies) and cell surface (intact cells, anti-CD8 antibodies) CD8. Images were collected in parallel using equal exposure times. (C) Cells expressing CD8 TMD* were lysed and subjected to carbonate extraction. Equivalent amounts of supernatant 1 (initial lysate supernatant), 2 and 3 (from sequential carbonate extractions) and the final membrane pellet were analysed by immunoblotting (IB) with antibodies against HA, calnexin (CNX), calrecticulin (CRT) and Hsp70. (D) Lysates of cells expressing CD8 TMD* or CD8 TMD*VN were treated with or without EndoH and analysed by immunoblotting with antibodies against HA, STT3B and actin. Closed arrowhead indicates N-glycosylated protein, open arrowhead indicates deglycosylated protein. (E) Cells expressing CD8 TMD* were permeabilised with digitonin or Triton-X 100 and labelled with antibodies against the extracellular (luminal) domain of CD8 and the cytoplasmic HA epitope. DNA was stained with DAPI. Scale bars: 10 µm.

    Journal: Journal of Cell Science

    Article Title: ERAD of proteins containing aberrant transmembrane domains requires ubiquitylation of cytoplasmic lysine residues

    doi: 10.1242/jcs.171215

    Figure Lengend Snippet: CD8 TMD* is integrated into the membrane with the correct topology. (A) Cartoon of CD8 chimeras used. (B) Stable HeLa cell lines expressing CD8 WT or CD8 TMD* were fixed and labelled for total (permeabilised cells, anti-HA antibodies) and cell surface (intact cells, anti-CD8 antibodies) CD8. Images were collected in parallel using equal exposure times. (C) Cells expressing CD8 TMD* were lysed and subjected to carbonate extraction. Equivalent amounts of supernatant 1 (initial lysate supernatant), 2 and 3 (from sequential carbonate extractions) and the final membrane pellet were analysed by immunoblotting (IB) with antibodies against HA, calnexin (CNX), calrecticulin (CRT) and Hsp70. (D) Lysates of cells expressing CD8 TMD* or CD8 TMD*VN were treated with or without EndoH and analysed by immunoblotting with antibodies against HA, STT3B and actin. Closed arrowhead indicates N-glycosylated protein, open arrowhead indicates deglycosylated protein. (E) Cells expressing CD8 TMD* were permeabilised with digitonin or Triton-X 100 and labelled with antibodies against the extracellular (luminal) domain of CD8 and the cytoplasmic HA epitope. DNA was stained with DAPI. Scale bars: 10 µm.

    Article Snippet: Reagents and antibodies Antibodies against CD8 and rabbit HA were from Sigma, antibodies against BAP31, LAMP1, actin, Hsp70 and β-tubulin were from AbCam, anti-ERGIC53 antibody was from Alexis, mouse anti-HA antibody was from Santa Cruz Biotechnology, anti-BiP antibody was from Cell Signaling, anti-CNX and -CRT antibodies for immunoblotting were from Stressgen, anti-CRT antibody for immunofluorescence was from Thermo Scientific and anti-GM130 antibody was from BD Biosciences.

    Techniques: Expressing, Staining

    Association of erlins with the SREBP regulatory machinery. (A) CoIP of SREBP pathway proteins with endogenous erlin-2 in lysates of HeLa cells grown with complete (F) or LD medium. Samples were probed by Western blotting as indicated. CANX, calnexin. 10 times more cell equivalents were loaded in IP lanes compared with input, except for erlin-2 blot (1 equivalent). Western blot images are typical of three separate repeats. Numbers below the panel indicate LD/F ratios of IP target proteins averaged from three experiments. P-values for LD versus complete (F): Scap, Srebp-1, and Srebp-2,

    Journal: The Journal of Cell Biology

    Article Title: Erlins restrict SREBP activation in the ER and regulate cellular cholesterol homeostasis

    doi: 10.1083/jcb.201305076

    Figure Lengend Snippet: Association of erlins with the SREBP regulatory machinery. (A) CoIP of SREBP pathway proteins with endogenous erlin-2 in lysates of HeLa cells grown with complete (F) or LD medium. Samples were probed by Western blotting as indicated. CANX, calnexin. 10 times more cell equivalents were loaded in IP lanes compared with input, except for erlin-2 blot (1 equivalent). Western blot images are typical of three separate repeats. Numbers below the panel indicate LD/F ratios of IP target proteins averaged from three experiments. P-values for LD versus complete (F): Scap, Srebp-1, and Srebp-2,

    Article Snippet: The resulting construct, pcDNA-Insig-1-mCherry, allowed expression of the red fluorescent fusion protein under control of a CMV promoter. pcDNA-CANX-mCherry was constructed by PCR amplifying full-length human calnexin (CANX) coding sequence from clone #4342723 (Thermo Fisher Scientific) with specific primers, introducing a SalI restriction site immediately after the last codon of the ORF for CANX.

    Techniques: Co-Immunoprecipitation Assay, Western Blot

    CNX knockdown partially inhibits CFTR folding. (A) Selective down-regulation of CNX with 100 nM siRNA in CFTR-expressing HeLa cells. Equal amounts of nonspecific (n.s.) and CNX siRNA–treated lysates were analyzed by immunoblotting. C and B indicate complex-glycosylated CFTR and core-glycosylated CFTR, respectively. (B) Folding yield of wt CFTR in CNX-depleted HeLa cells was measured by pulse-chase experiments ( n = 3). (C) Effect of CNX knockdown on the cell surface density of CFTR in HeLa cells ( n = 3). Error bars indicate mean ± SEM.

    Journal: The Journal of Cell Biology

    Article Title: N-glycans are direct determinants of CFTR folding and stability in secretory and endocytic membrane traffic

    doi: 10.1083/jcb.200808124

    Figure Lengend Snippet: CNX knockdown partially inhibits CFTR folding. (A) Selective down-regulation of CNX with 100 nM siRNA in CFTR-expressing HeLa cells. Equal amounts of nonspecific (n.s.) and CNX siRNA–treated lysates were analyzed by immunoblotting. C and B indicate complex-glycosylated CFTR and core-glycosylated CFTR, respectively. (B) Folding yield of wt CFTR in CNX-depleted HeLa cells was measured by pulse-chase experiments ( n = 3). (C) Effect of CNX knockdown on the cell surface density of CFTR in HeLa cells ( n = 3). Error bars indicate mean ± SEM.

    Article Snippet: siRNA transfection CNX depletion of HeLa cells stably expressing CFTR-3HA was achieved by the CNX-specific ON-TARGET plus SMARTpool siRNA (100 nM final concentration; Thermo Fisher Scientific) using Oligofectamine (Invitrogen) transfection.

    Techniques: Expressing, Pulse Chase