Journal: BMC Nephrology

Article Title: Increasing extracellular matrix collagen level and MMP activity induces cyst development in polycystic kidney disease

doi: 10.1186/1471-2369-13-109

Figure Lengend Snippet: MT1-MMP induces cell proliferation and cyst growth in 3D collagen I gel culture. A and B : RT-PCR ( A ) and western blot ( B ) analyses of the MT1-MMP mRNA and protein expression in parental and MT1-MMP overexpressed MDCK cells. C and D : Western blot analysis of the PCNA expression in parental and MT1-MMP overexpressed MDCK cells that were cultured under 2D ( C ) or 3D ( D ) conditions. E . The diameters of the cysts that were formed from parental MDCK cells or MT1-MMP overexpressing MDCK cells treated with or without Doxycycline for 9 days in 2.5 mg/ml collagen I gels. **p < 0.005 versus MDCK cells. *p < 0.005 versus MDCK MT1-MMP cells.

Article Snippet: MDCK cells were obtained from American Type Culture Collection (Manassas, VA) and grown in Dulbecco’s modified Eagle’s medium (DMEM)/Ham’s F12 (50:50) medium supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 U/ml streptomycin (Mediatech, Manassas, VA). pMSCVGFP-MT1-MMP expression vector is a gift from Dr. Zonghan Dai (Texas Tech University HSC).

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing, Cell Culture

Journal: BMC Evolutionary Biology

Article Title: Whole-proteome phylogeny of large dsDNA viruses and parvoviruses through a composition vector method related to dynamical language model

doi: 10.1186/1471-2148-10-192

Figure Lengend Snippet: The NJ tree of 30 parvovirus genomes based on the all protein sequences using the modified DL method for K = 4 .

Article Snippet: The species in the subfamily Parvovirinae are: Aleutian mink disease virus (ADMV, NC_001662) in the genus Amdovirus ; Minute virus of canines (MVC, NC_004442) in the genus Bocavirus ; Adeno-associated virus 1 (AAV1, NC_002077), Adeno-associated virus 2 (AAV2, NC_001401), Adeno-associated virus 3 (AAV3, NC_001729), Adeno-associated virus 4 (AAV4, NC_001829), Adeno-associated virus 5 (AAV5, NC_006152), Adeno-associated virus 7 (AAV7, NC_006260), Adeno-associated virus 8 (AAV8, NC_006261), Avian adeno-associated virus ATCC VR-865 (AAAVa, NC_004828), Avian adeno-associated virus strain DA-1 (AAAVd, NC_006263), Bovine adeno-associated virus (BAAV, NC_005889), Bovine parvovirus -2 (BPV2, NC_006259), Goose parvovirus (GPV, NC_001701) and Muscovy duck parvovirus (MDPV, NC_006147) in the genus Dependovirus ; B19 virus (B19V, NC_000883) in the genus Erythrovirus ; Canine parvovirus (CPV, NC_001539), LuIII parvovirus (LuIIIV, NC_004713), Mouse parvovirus 3 (MPV, NC_008185) and Porcine parvovirus (PPV, NC_001718) in the genus Parvovirus .

Techniques: Modification