calprotectin elisa  (Immundiagnostik AG)


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    Immundiagnostik AG calprotectin elisa
    Increased inflammation and decreased ILCs in DJ-1 −/− mice compared with WT. (a) <t>Calprotectin</t> level (mean±SEM) measured by <t>ELISA</t> in WT and DJ-1 −/− mice fecal samples (n=5). (b) DJ-1 −/− mice have significantly higher production of the MCP-1 (mean±SEM) cytokine measurement from the feces. p values (0.04 Student’s t-test). * represents the p value of
    Calprotectin Elisa, supplied by Immundiagnostik AG, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 3 article reviews
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    calprotectin elisa - by Bioz Stars, 2022-09
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    Images

    1) Product Images from "DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)"

    Article Title: DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)

    Journal: bioRxiv

    doi: 10.1101/776005

    Increased inflammation and decreased ILCs in DJ-1 −/− mice compared with WT. (a) Calprotectin level (mean±SEM) measured by ELISA in WT and DJ-1 −/− mice fecal samples (n=5). (b) DJ-1 −/− mice have significantly higher production of the MCP-1 (mean±SEM) cytokine measurement from the feces. p values (0.04 Student’s t-test). * represents the p value of
    Figure Legend Snippet: Increased inflammation and decreased ILCs in DJ-1 −/− mice compared with WT. (a) Calprotectin level (mean±SEM) measured by ELISA in WT and DJ-1 −/− mice fecal samples (n=5). (b) DJ-1 −/− mice have significantly higher production of the MCP-1 (mean±SEM) cytokine measurement from the feces. p values (0.04 Student’s t-test). * represents the p value of

    Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay

    2) Product Images from "Enriched Environmental Conditions Modify the Gut Microbiome Composition and Fecal Markers of Inflammation in Parkinson’s Disease"

    Article Title: Enriched Environmental Conditions Modify the Gut Microbiome Composition and Fecal Markers of Inflammation in Parkinson’s Disease

    Journal: Frontiers in Neuroscience

    doi: 10.3389/fnins.2019.01032

    Enriched environment condition dampens the production of inflammatory calprotectin protein and pro-inflammatory cytokines in the feces. (A) The colon fecal S100A8/S100A9 (Calprotectin, MRP 8/14) protein was estimated using ELISA. Calprotectin levels were significantly different between SE and EE for the WT and SNCA-TG mice, respectively. One-way ANOVA and a post hoc Tukey test was performed to find the significance levels between different groups (SE and EE for WT and SNCA-TG). (B) The pro-inflammatory cytokines IFN-γ, IL-12p70, IL-1β, and MCP-1 were estimated using the flow cytometry based-multiplex cytokine assay. IFN-γ and IL-12p70 were significantly different between SE and EE for the WT and SNCA-TG mice, respectively. IL-1β was significantly different between WT SE and EE condition, however, SNCA-TG EE condition tended to have lower IL-1β cytokine, however, no statistical significance was reached. MCP-1 was statistically significant in the SE state between WT and SNCA-TG, however, in EE state it was tended to higher (no statistical difference). All the data are presented in means ± SEM of n = 4–5 feces samples per group. p -Value was considered significant if it was less than or equal to 0.05 ( ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001) using One-way ANOVA and a post hoc Tukey test or/and Student’s t -test unpaired t -test.
    Figure Legend Snippet: Enriched environment condition dampens the production of inflammatory calprotectin protein and pro-inflammatory cytokines in the feces. (A) The colon fecal S100A8/S100A9 (Calprotectin, MRP 8/14) protein was estimated using ELISA. Calprotectin levels were significantly different between SE and EE for the WT and SNCA-TG mice, respectively. One-way ANOVA and a post hoc Tukey test was performed to find the significance levels between different groups (SE and EE for WT and SNCA-TG). (B) The pro-inflammatory cytokines IFN-γ, IL-12p70, IL-1β, and MCP-1 were estimated using the flow cytometry based-multiplex cytokine assay. IFN-γ and IL-12p70 were significantly different between SE and EE for the WT and SNCA-TG mice, respectively. IL-1β was significantly different between WT SE and EE condition, however, SNCA-TG EE condition tended to have lower IL-1β cytokine, however, no statistical significance was reached. MCP-1 was statistically significant in the SE state between WT and SNCA-TG, however, in EE state it was tended to higher (no statistical difference). All the data are presented in means ± SEM of n = 4–5 feces samples per group. p -Value was considered significant if it was less than or equal to 0.05 ( ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001) using One-way ANOVA and a post hoc Tukey test or/and Student’s t -test unpaired t -test.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Mouse Assay, Flow Cytometry, Cytometry, Multiplex Assay, Cytokine Assay

    3) Product Images from "DJ-1 (Park7) affects the gut microbiome, metabolites and the development of innate lymphoid cells (ILCs)"

    Article Title: DJ-1 (Park7) affects the gut microbiome, metabolites and the development of innate lymphoid cells (ILCs)

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-72903-w

    Increased inflammation and decreased ILCs in DJ-1 −/− mice. (a) Calprotectin level (mean ± SEM) measured by ELISA in DJ-1 +/+ and DJ-1 −/− mice fecal samples (n = 5). (b) DJ-1 −/− mice have significantly higher production of the MCP-1 (mean ± SEM) cytokine measurement from the feces. * represents the p value of
    Figure Legend Snippet: Increased inflammation and decreased ILCs in DJ-1 −/− mice. (a) Calprotectin level (mean ± SEM) measured by ELISA in DJ-1 +/+ and DJ-1 −/− mice fecal samples (n = 5). (b) DJ-1 −/− mice have significantly higher production of the MCP-1 (mean ± SEM) cytokine measurement from the feces. * represents the p value of

    Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay

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    Immundiagnostik AG calprotectin
    Pearson correlation analysis of S100A12, fecal <t>calprotectin,</t> and hBD2 with fecal microbiota. (a)-(b) Correlation between fCP and total bacterial CFU/g feces (a) and E. coli CFU/g feces (b). (c)-(d) Correlation between S100A12 and total bacterial CFU/g feces (c) and E. coli CFU/g feces (d). (e)-(f) Correlation between hBD2 and total bacterial CFU/g feces (e) and E. coli CFU/g feces (f). 221, 156, and 57 fecal samples were analyzed for fCP, S100A12, and hBD2, respectively.
    Calprotectin, supplied by Immundiagnostik AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
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    86
    Immundiagnostik AG rat calprotectin
    Time course of AMP expression in rat serum ( n = 6/time point). Secretome MNC s derived from irradiated MNC s after 24 h were injected into healthy male rats. Rat serum samples were obtained at 2, 12 and 24 h after injection. AMP levels were measured by rat‐specific ELISA s. A highly significant increase in rat <t>calprotectin</t> 24 h after injection of irradiated MNC ‐sec compared to medium was detected. All values are shown as mean ± SD . P values
    Rat Calprotectin, supplied by Immundiagnostik AG, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat calprotectin/product/Immundiagnostik AG
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Pearson correlation analysis of S100A12, fecal calprotectin, and hBD2 with fecal microbiota. (a)-(b) Correlation between fCP and total bacterial CFU/g feces (a) and E. coli CFU/g feces (b). (c)-(d) Correlation between S100A12 and total bacterial CFU/g feces (c) and E. coli CFU/g feces (d). (e)-(f) Correlation between hBD2 and total bacterial CFU/g feces (e) and E. coli CFU/g feces (f). 221, 156, and 57 fecal samples were analyzed for fCP, S100A12, and hBD2, respectively.

    Journal: BioMed Research International

    Article Title: S100A12 and hBD2 Correlate with the Composition of the Fecal Microflora in ELBW Infants and Expansion of E. coli Is Associated with NEC

    doi: 10.1155/2013/150372

    Figure Lengend Snippet: Pearson correlation analysis of S100A12, fecal calprotectin, and hBD2 with fecal microbiota. (a)-(b) Correlation between fCP and total bacterial CFU/g feces (a) and E. coli CFU/g feces (b). (c)-(d) Correlation between S100A12 and total bacterial CFU/g feces (c) and E. coli CFU/g feces (d). (e)-(f) Correlation between hBD2 and total bacterial CFU/g feces (e) and E. coli CFU/g feces (f). 221, 156, and 57 fecal samples were analyzed for fCP, S100A12, and hBD2, respectively.

    Article Snippet: Calprotectin, hBD2, and S100A12 Analysis in Fecal Samples Fecal calprotectin (Immundiagnostik, Bensheim, Germany) and hBD2 (Immundiagnostik, Bensheim, Germany) concentrations were determined by enzyme-linked immunosorbent assays (ELISA) [ , ] and S100A12 concentrations by double-sandwich ELISA as described previously [ ].

    Techniques:

    Changes in fecal calprotectin concentration in prospective study. * P = 0.012.

    Journal: BioMed Research International

    Article Title: Therapeutic Efficacy of pH-Dependent Release Formulation of Mesalazine on Active Ulcerative Colitis Resistant to Time-Dependent Release Formulation: Analysis of Fecal Calprotectin Concentration

    doi: 10.1155/2014/342751

    Figure Lengend Snippet: Changes in fecal calprotectin concentration in prospective study. * P = 0.012.

    Article Snippet: The calprotectin concentration was determined using a quantitative enzyme-linked immunosorbent assay (PhiCal, Immundiagnostik, Germany).

    Techniques: Concentration Assay

    Individual measurement results of urinary calprotectin and calprotectin/creatinine ratio of healthy controls, prerenal acute kidney injury (AKI), and intrinsic AKI of the complete study population (A) and all subjects without urinary tract infection (UTI) (B). The data are presented as scatter plots (logarithmic y axis; medians are indicated by horizontal lines). Calprotectin concentrations and calprotectin/creatinine ratios did not significantly differ between healthy controls and prerenal AKI but were significantly higher in intrinsic AKI than in prerenal AKI and controls ( P

    Journal: Clinical Journal of the American Society of Nephrology : CJASN

    Article Title: Urinary Calprotectin and the Distinction between Prerenal and Intrinsic Acute Kidney Injury

    doi: 10.2215/CJN.02490311

    Figure Lengend Snippet: Individual measurement results of urinary calprotectin and calprotectin/creatinine ratio of healthy controls, prerenal acute kidney injury (AKI), and intrinsic AKI of the complete study population (A) and all subjects without urinary tract infection (UTI) (B). The data are presented as scatter plots (logarithmic y axis; medians are indicated by horizontal lines). Calprotectin concentrations and calprotectin/creatinine ratios did not significantly differ between healthy controls and prerenal AKI but were significantly higher in intrinsic AKI than in prerenal AKI and controls ( P

    Article Snippet: Urine concentrations of calprotectin were quantified using an ELISA kit (PhiCal® Calprotectin, catalog number K 6935; Immundiagnostik AG, Bensheim, Germany) according to the manufacturer's protocol.

    Techniques: Infection

    Histologic findings in periodic acid Schiff reaction (PAS) staining (×100; scale bars, 100 μm) and immunohistochemistry with an antibody to the S100A8/A9 heterocomplex (×200, brown; scale bars, 100 μm) in pyelonephritis (A and B; urinary calprotectin, 8232 ng/ml), lupus nephritis (C and D; urinary calprotectin, 1422 ng/ml), and a patient fulfilling the clinical criteria of prerenal disease (E and F; urinary calprotectin 3 ng/ml). (A) PAS staining in pyelonephritis reveals marked tubulointerstitial nephritis, patchy interstitial inflammation by polymorphonuclear leukocytes (black arrow), and acute tubular injury with a dense inflammatory infiltrate (white arrow). (B) Neutrophils occurring in tubular microabscesses are strongly positive in the calprotectin staining (black arrow). (C) PAS staining in lupus nephritis shows two glomeruli with mild to moderate mesangial hypercellularity segmentally involving the glomerular tuft and increasing the mesangial matrix. Moreover, there is a small segmental lesion with endocapillary proliferation (black arrow). Tubular injury is mild with focal atrophy of tubular epithelial cells. (D) Calprotectin staining evoked positive signals in both the intraglomerular space (black arrow) and, to a lesser extent, in the interstitium (white arrows). (E) PAS staining in the kidney fulfilling the clinical criteria of prerenal acute kidney injury displays glomeruli without any histologic abnormalities. The glomerular tuft is normocellular, the glomerular capillaries are fully patent. Discrete tubular findings with mild widening of the tubular lumina (black arrow). (F) Calprotectin staining is negative.

    Journal: Clinical Journal of the American Society of Nephrology : CJASN

    Article Title: Urinary Calprotectin and the Distinction between Prerenal and Intrinsic Acute Kidney Injury

    doi: 10.2215/CJN.02490311

    Figure Lengend Snippet: Histologic findings in periodic acid Schiff reaction (PAS) staining (×100; scale bars, 100 μm) and immunohistochemistry with an antibody to the S100A8/A9 heterocomplex (×200, brown; scale bars, 100 μm) in pyelonephritis (A and B; urinary calprotectin, 8232 ng/ml), lupus nephritis (C and D; urinary calprotectin, 1422 ng/ml), and a patient fulfilling the clinical criteria of prerenal disease (E and F; urinary calprotectin 3 ng/ml). (A) PAS staining in pyelonephritis reveals marked tubulointerstitial nephritis, patchy interstitial inflammation by polymorphonuclear leukocytes (black arrow), and acute tubular injury with a dense inflammatory infiltrate (white arrow). (B) Neutrophils occurring in tubular microabscesses are strongly positive in the calprotectin staining (black arrow). (C) PAS staining in lupus nephritis shows two glomeruli with mild to moderate mesangial hypercellularity segmentally involving the glomerular tuft and increasing the mesangial matrix. Moreover, there is a small segmental lesion with endocapillary proliferation (black arrow). Tubular injury is mild with focal atrophy of tubular epithelial cells. (D) Calprotectin staining evoked positive signals in both the intraglomerular space (black arrow) and, to a lesser extent, in the interstitium (white arrows). (E) PAS staining in the kidney fulfilling the clinical criteria of prerenal acute kidney injury displays glomeruli without any histologic abnormalities. The glomerular tuft is normocellular, the glomerular capillaries are fully patent. Discrete tubular findings with mild widening of the tubular lumina (black arrow). (F) Calprotectin staining is negative.

    Article Snippet: Urine concentrations of calprotectin were quantified using an ELISA kit (PhiCal® Calprotectin, catalog number K 6935; Immundiagnostik AG, Bensheim, Germany) according to the manufacturer's protocol.

    Techniques: Staining, Immunohistochemistry

    Study flow diagram. Diagnostic performance of urinary calprotectin using a cut off level of 300 ng/ml for the assignment to prerenal and intrinsic acute kidney injury (AKI).

    Journal: Clinical Journal of the American Society of Nephrology : CJASN

    Article Title: Urinary Calprotectin and the Distinction between Prerenal and Intrinsic Acute Kidney Injury

    doi: 10.2215/CJN.02490311

    Figure Lengend Snippet: Study flow diagram. Diagnostic performance of urinary calprotectin using a cut off level of 300 ng/ml for the assignment to prerenal and intrinsic acute kidney injury (AKI).

    Article Snippet: Urine concentrations of calprotectin were quantified using an ELISA kit (PhiCal® Calprotectin, catalog number K 6935; Immundiagnostik AG, Bensheim, Germany) according to the manufacturer's protocol.

    Techniques: Flow Cytometry, Diagnostic Assay

    Time course of AMP expression in rat serum ( n = 6/time point). Secretome MNC s derived from irradiated MNC s after 24 h were injected into healthy male rats. Rat serum samples were obtained at 2, 12 and 24 h after injection. AMP levels were measured by rat‐specific ELISA s. A highly significant increase in rat calprotectin 24 h after injection of irradiated MNC ‐sec compared to medium was detected. All values are shown as mean ± SD . P values

    Journal: European Journal of Clinical Investigation

    Article Title: Dying blood mononuclear cell secretome exerts antimicrobial activity

    doi: 10.1111/eci.12667

    Figure Lengend Snippet: Time course of AMP expression in rat serum ( n = 6/time point). Secretome MNC s derived from irradiated MNC s after 24 h were injected into healthy male rats. Rat serum samples were obtained at 2, 12 and 24 h after injection. AMP levels were measured by rat‐specific ELISA s. A highly significant increase in rat calprotectin 24 h after injection of irradiated MNC ‐sec compared to medium was detected. All values are shown as mean ± SD . P values

    Article Snippet: Our findings revealed that naive rats exposed to xenogeneic GMP MNC‐sec showed a marked increase in rat calprotectin in a time‐dependent fashion.

    Techniques: Expressing, Derivative Assay, Irradiation, Injection, Enzyme-linked Immunosorbent Assay, Size-exclusion Chromatography