calcium free phosphate buffered saline pbs  (Bio-Rad)

 
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  • 99
    Name:
    Phosphate Buffered Saline PBS
    Description:
    11 34 g makes 1 L reconstituted buffer
    Catalog Number:
    31098
    Price:
    None
    Category:
    Systemic Autoimmune Testing
    Buy from Supplier


    Structured Review

    Bio-Rad calcium free phosphate buffered saline pbs
    Phosphate Buffered Saline PBS
    11 34 g makes 1 L reconstituted buffer
    https://www.bioz.com/result/calcium free phosphate buffered saline pbs/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calcium free phosphate buffered saline pbs - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Red blood cells are dynamic reservoirs of cytokines
    Article Snippet: .. Binding of recombinant cytokines to RBCs Bio-Plex recombinant cytokine standards (27-plex and 21-plex human cytokine panels, Bio-Rad, Hercules, CA) contain cytokines at variable concentrations so these were reconstituted and diluted in PBS according to manufacturers instructions to produce standard-3 (27-plex and 21-plex human cytokine panels, Bio-Rad). .. Bio-Plex standard-3 was then diluted 1:8 in PBS with RBCs at a final concentration of 400 × 106 cells/mL and the cell suspension was incubated for 24 hours (37 °C).

    Purification:

    Article Title: Mapping Protective Regions on a Three-Dimensional Model of the Moraxella catarrhalis Vaccine Antigen Oligopeptide Permease A
    Article Snippet: .. In brief, 1 mg of His-tagged OppA was purified, buffer exchanged into 1 ml of 1× PBS (Bio-Rad, Hercules, CA), and sent to Covance (Denver, PA) to perform a 59-day immunization protocol. .. Each of 2 animals was given an initial subcutaneous injection of 250 μg OppA emulsified (1:1 [vol/vol]) in complete Freund's adjuvant.

    Article Title: LidA, a Translocated Substrate of the Legionella pneumophila Type IV Secretion System, Interferes with the Early Secretory Pathway
    Article Snippet: .. Sixty microliters of postexponential L. pneumophila expressing green fluorescent protein (GFP) was washed once and resuspended in 5 ml of phosphate-buffered saline (PBS) containing either 1% Triton X-100 (Bio-Rad), 1% CHAPS {3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate; Sigma}, 1% octylglucoside (Calbiochem), or 1% purified digitonin (Calbiochem) and incubated for 20 min at room temperature with intermittent vortexing. ..

    Incubation:

    Article Title: LidA, a Translocated Substrate of the Legionella pneumophila Type IV Secretion System, Interferes with the Early Secretory Pathway
    Article Snippet: .. Sixty microliters of postexponential L. pneumophila expressing green fluorescent protein (GFP) was washed once and resuspended in 5 ml of phosphate-buffered saline (PBS) containing either 1% Triton X-100 (Bio-Rad), 1% CHAPS {3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate; Sigma}, 1% octylglucoside (Calbiochem), or 1% purified digitonin (Calbiochem) and incubated for 20 min at room temperature with intermittent vortexing. ..

    Article Title: The development and use of an ELISA-based method to follow the distribution of cellulase monocomponents during the hydrolysis of pretreated corn stover
    Article Snippet: .. Following another washing step, the third antibody, a commercial GAR-AP (Bio-Rad) diluted in PBS with 1% (w/v) BSA, was added to the wells and incubated for another hour. .. After a final washing step, 35 mg/ml of p -nitrophenylphosphate (Bio-Rad), a substrate for alkaline phosphatase (AP), was added to the wells and the plate was incubated at room temperature for 30 minutes or until sufficient colour had developed.

    Expressing:

    Article Title: LidA, a Translocated Substrate of the Legionella pneumophila Type IV Secretion System, Interferes with the Early Secretory Pathway
    Article Snippet: .. Sixty microliters of postexponential L. pneumophila expressing green fluorescent protein (GFP) was washed once and resuspended in 5 ml of phosphate-buffered saline (PBS) containing either 1% Triton X-100 (Bio-Rad), 1% CHAPS {3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate; Sigma}, 1% octylglucoside (Calbiochem), or 1% purified digitonin (Calbiochem) and incubated for 20 min at room temperature with intermittent vortexing. ..

    Recombinant:

    Article Title: Red blood cells are dynamic reservoirs of cytokines
    Article Snippet: .. Bio-Plex recombinant cytokine standards (27-plex and 21-plex human cytokine panels, Bio-Rad, Hercules, CA) contain cytokines at variable concentrations so these were reconstituted and diluted in PBS according to manufacturers instructions to produce standard-3 (27-plex and 21-plex human cytokine panels, Bio-Rad). .. Bio-Plex standard-3 was then diluted 1:8 in PBS with RBCs at a final concentration of 400 × 106 cells/mL and the cell suspension was incubated for 24 hours (37 °C).

    Article Title: Red blood cells are dynamic reservoirs of cytokines
    Article Snippet: .. Binding of recombinant cytokines to RBCs Bio-Plex recombinant cytokine standards (27-plex and 21-plex human cytokine panels, Bio-Rad, Hercules, CA) contain cytokines at variable concentrations so these were reconstituted and diluted in PBS according to manufacturers instructions to produce standard-3 (27-plex and 21-plex human cytokine panels, Bio-Rad). .. Bio-Plex standard-3 was then diluted 1:8 in PBS with RBCs at a final concentration of 400 × 106 cells/mL and the cell suspension was incubated for 24 hours (37 °C).

    DC Protein Assay:

    Article Title: Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research
    Article Snippet: .. A dilution series of 0 to 10% of Optiprep in PBS was obtained as a standard curve for performing absorbance readings with DC Protein assay (Bio-Rad, Hercules, California, USA), according to manufacturer’s instructions. ..

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