cadherin 11  (Thermo Fisher)


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    Name:
    TII Type 2 Water System Smart Distribution pump
    Description:
    Integrate the accessories for the Thermo Scientific Barnstear TII Type 2 Water System such as distribution pumps and UV lamps with the system itself to create a more complete lab solution
    Catalog Number:
    AY1406X2
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    Category:
    Instruments and Equipment
    Applications:
    Lab Equipment|Water Purification
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    Structured Review

    Thermo Fisher cadherin 11
    The distribution of <t>cadherin</t> 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Integrate the accessories for the Thermo Scientific Barnstear TII Type 2 Water System such as distribution pumps and UV lamps with the system itself to create a more complete lab solution
    https://www.bioz.com/result/cadherin 11/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cadherin 11 - by Bioz Stars, 2021-05
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    Images

    1) Product Images from "Genomic Locus Modulating IOP in the BXD RI Mouse Strains"

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200190

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Figure Legend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Techniques Used: Staining

    2) Product Images from "Genomic Locus Modulating IOP in the BXD RI Mouse Strains"

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200190

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Figure Legend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Techniques Used: Staining

    3) Product Images from "Epiblast cells that express MyoD recruit pluripotent cells to the skeletal muscle lineage"

    Article Title: Epiblast cells that express MyoD recruit pluripotent cells to the skeletal muscle lineage

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.200309152

    Immunofluorescence localization of cadherins and β -catenin in G8 po s and G8 neg cultures. G8 pos and G8 neg cells were isolated from stages 3 to 5 epiblasts, cultured for 5 d, and double labeled with antibodies to N- and E-cadherin (A–D), or single labeled with antibodies to cadherin 11 and β-catenin. Most G8 pos cells expressed N- but not E-cadherin (A and B). G8 neg cultures contained cells with E- but not N-cadherin (C and D). G8 neg cells stained with antibodies to β-catenin (E) and cadherin 11 (F). Bar, 10 μm.
    Figure Legend Snippet: Immunofluorescence localization of cadherins and β -catenin in G8 po s and G8 neg cultures. G8 pos and G8 neg cells were isolated from stages 3 to 5 epiblasts, cultured for 5 d, and double labeled with antibodies to N- and E-cadherin (A–D), or single labeled with antibodies to cadherin 11 and β-catenin. Most G8 pos cells expressed N- but not E-cadherin (A and B). G8 neg cultures contained cells with E- but not N-cadherin (C and D). G8 neg cells stained with antibodies to β-catenin (E) and cadherin 11 (F). Bar, 10 μm.

    Techniques Used: Immunofluorescence, Isolation, Cell Culture, Labeling, Staining

    4) Product Images from "Genomic Locus Modulating IOP in the BXD RI Mouse Strains"

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200190

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Figure Legend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Techniques Used: Staining

    5) Product Images from "Genomic Locus Modulating IOP in the BXD RI Mouse Strains"

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200190

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Figure Legend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Techniques Used: Staining

    6) Product Images from "Genomic Locus Modulating IOP in the BXD RI Mouse Strains"

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200190

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Figure Legend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Techniques Used: Staining

    7) Product Images from "Genomic Locus Modulating IOP in the BXD RI Mouse Strains"

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200190

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Figure Legend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Techniques Used: Staining

    8) Product Images from "Genomic Locus Modulating IOP in the BXD RI Mouse Strains"

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200190

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Figure Legend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Techniques Used: Staining

    9) Product Images from "Co-culture with mesenchymal stromal cells increases proliferation and maintenance of haematopoietic progenitor cells"

    Article Title: Co-culture with mesenchymal stromal cells increases proliferation and maintenance of haematopoietic progenitor cells

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/j.1582-4934.2009.00776.x

    Specific knockdown of adhesion proteins by siRNA. Knockdown of N-cadherin (N-CDH), cadherin-11 (CDH11), integrin beta 1 (ITGB1), CD44 and MAPK1 in MSC was verified after 2 days by Western blot analysis (A). Knockdown of ITGB1, VCAM1 and Jagged1 was validated after 2 days by quantitative RT-PCR (B; **= P
    Figure Legend Snippet: Specific knockdown of adhesion proteins by siRNA. Knockdown of N-cadherin (N-CDH), cadherin-11 (CDH11), integrin beta 1 (ITGB1), CD44 and MAPK1 in MSC was verified after 2 days by Western blot analysis (A). Knockdown of ITGB1, VCAM1 and Jagged1 was validated after 2 days by quantitative RT-PCR (B; **= P

    Techniques Used: Western Blot, Quantitative RT-PCR

    10) Product Images from "Genomic Locus Modulating IOP in the BXD RI Mouse Strains"

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200190

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Figure Legend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Techniques Used: Staining

    11) Product Images from "Cadherin-11 regulates cell-cell tension necessary for calcific nodule formation by valvular myofibroblasts"

    Article Title: Cadherin-11 regulates cell-cell tension necessary for calcific nodule formation by valvular myofibroblasts

    Journal: Arteriosclerosis, thrombosis, and vascular biology

    doi: 10.1161/ATVBAHA.112.300278

    TGF-β1 incubation for 24 h increases cadherin-11 expression in AVICs. (A) pPCR reveals a 1.58 fold increase in cadherin-11 mRNA in samples treated with TGF-β1 and a decrease in cadherin-11 mRNA with inhibition of MEK1/2. (B) Immunostaining shows cadherin-11 in TGF-β1 groups with minimal to no stain in other treatment groups. Scalebar = 10 µm. All error bars indicate standard error of the mean. * indicates significant difference (p
    Figure Legend Snippet: TGF-β1 incubation for 24 h increases cadherin-11 expression in AVICs. (A) pPCR reveals a 1.58 fold increase in cadherin-11 mRNA in samples treated with TGF-β1 and a decrease in cadherin-11 mRNA with inhibition of MEK1/2. (B) Immunostaining shows cadherin-11 in TGF-β1 groups with minimal to no stain in other treatment groups. Scalebar = 10 µm. All error bars indicate standard error of the mean. * indicates significant difference (p

    Techniques Used: Incubation, Expressing, Inhibition, Immunostaining, Staining

    Cadherin-11 generates intercellular tension through αSMA that enables calcific nodule morphogenesis. (A) Wound assay reveals strength of intercellular connection which correlates to wound area. At normal Ca 2+ levels, with all cadherins functional, TGF-β1, U0126, and U0126 + TGF-β1 treatments all increased wound area size due to increased αSMA expression. However, at low Ca 2+ levels, where cadherin-11 is still functional and others are not, TGF-β1 treated cells created a large wound. (B) siRNA knockdown of cadherin-11 in physiologic Ca 2+ media decreases TGF-β1 initiated wound area to control levels. (C) siRNA knockdown of cadherin-11 prevents calcific nodules. Inset Western blots show that cadherin-11 is knocked down at the time of the wound assay and calcific nodule experiments. All error bars indicate standard error of the mean. * indicates significant difference (p
    Figure Legend Snippet: Cadherin-11 generates intercellular tension through αSMA that enables calcific nodule morphogenesis. (A) Wound assay reveals strength of intercellular connection which correlates to wound area. At normal Ca 2+ levels, with all cadherins functional, TGF-β1, U0126, and U0126 + TGF-β1 treatments all increased wound area size due to increased αSMA expression. However, at low Ca 2+ levels, where cadherin-11 is still functional and others are not, TGF-β1 treated cells created a large wound. (B) siRNA knockdown of cadherin-11 in physiologic Ca 2+ media decreases TGF-β1 initiated wound area to control levels. (C) siRNA knockdown of cadherin-11 prevents calcific nodules. Inset Western blots show that cadherin-11 is knocked down at the time of the wound assay and calcific nodule experiments. All error bars indicate standard error of the mean. * indicates significant difference (p

    Techniques Used: Functional Assay, Expressing, Western Blot

    Cadherin-11 and αSMA expression are increased in calcified human aortic valve leaflets. (A) Immunostaining of a non-calcified leaflet reveals cadherin-11 expression along the periphery of the leaflet, sparse αSMA staining, and very little calcification indicated by the von Kossa stain. (B) Calcified leaflet shows enriched cadherin-11 and αSMA co-localization in areas of significant calcification (panels a and b), as seen in the von Kossa stain, but not in areas where calcification is less intense (panel c). (C) mRNA for cadherin-11 and αSMA are increased in the calcified leaflet (n=1) compared to the non-calcified leaflets (n=2). Scalebar = 500 µm for von Kossa; = 100 µm for immunofluorescence.
    Figure Legend Snippet: Cadherin-11 and αSMA expression are increased in calcified human aortic valve leaflets. (A) Immunostaining of a non-calcified leaflet reveals cadherin-11 expression along the periphery of the leaflet, sparse αSMA staining, and very little calcification indicated by the von Kossa stain. (B) Calcified leaflet shows enriched cadherin-11 and αSMA co-localization in areas of significant calcification (panels a and b), as seen in the von Kossa stain, but not in areas where calcification is less intense (panel c). (C) mRNA for cadherin-11 and αSMA are increased in the calcified leaflet (n=1) compared to the non-calcified leaflets (n=2). Scalebar = 500 µm for von Kossa; = 100 µm for immunofluorescence.

    Techniques Used: Expressing, Immunostaining, Staining, Immunofluorescence

    12) Product Images from "Genomic Locus Modulating IOP in the BXD RI Mouse Strains"

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200190

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Figure Legend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Techniques Used: Staining

    13) Product Images from "Cadherin-11, a Marker of the Mesenchymal Phenotype, Regulates Glioblastoma Cell Migration and Survival In Vivo"

    Article Title: Cadherin-11, a Marker of the Mesenchymal Phenotype, Regulates Glioblastoma Cell Migration and Survival In Vivo

    Journal: Molecular Cancer Research

    doi: 10.1158/1541-7786.MCR-11-0457

    Knockdown of cadherin-11 inhibits U-87 MG glioma cell survival in a heterotopic xenograft in vivo . A, U-87 MG cells expressing control or cadherin-11 shRNA and GFP were injected into the flank of athymic nude mice and imaged for brightfield and GFP fluorescence
    Figure Legend Snippet: Knockdown of cadherin-11 inhibits U-87 MG glioma cell survival in a heterotopic xenograft in vivo . A, U-87 MG cells expressing control or cadherin-11 shRNA and GFP were injected into the flank of athymic nude mice and imaged for brightfield and GFP fluorescence

    Techniques Used: In Vivo, Expressing, shRNA, Injection, Mouse Assay, Fluorescence

    Knockdown of cadherin-11 reduces survival of glioma cells at an orthotopic site in vivo . U-87 MG (A) and LN-229 cells (C) co-expressing control or cadherin-11 shRNA and GFP were injected intracranially into the striatum of athymic nude mice. 3 weeks following
    Figure Legend Snippet: Knockdown of cadherin-11 reduces survival of glioma cells at an orthotopic site in vivo . U-87 MG (A) and LN-229 cells (C) co-expressing control or cadherin-11 shRNA and GFP were injected intracranially into the striatum of athymic nude mice. 3 weeks following

    Techniques Used: In Vivo, Expressing, shRNA, Injection, Mouse Assay

    Knockdown of cadherin-11 inhibits migration of glioma cells in scratch wound assays in vitro . Data represent mean ± SE of at least 3 independent experiments. U-87 MG (A) and LN-229 (B) cells infected with lentivirus encoding control or cadherin-11
    Figure Legend Snippet: Knockdown of cadherin-11 inhibits migration of glioma cells in scratch wound assays in vitro . Data represent mean ± SE of at least 3 independent experiments. U-87 MG (A) and LN-229 (B) cells infected with lentivirus encoding control or cadherin-11

    Techniques Used: Migration, In Vitro, Infection

    Knockdown of cadherin-11 inhibits migration of glioma cells in spot assays in vitro . Data represent mean ± SE of at least 3 independent experiments. U-87 MG (A) and LN-229 (B) cells infected with lentivirus encoding control or cadherin-11 shRNA
    Figure Legend Snippet: Knockdown of cadherin-11 inhibits migration of glioma cells in spot assays in vitro . Data represent mean ± SE of at least 3 independent experiments. U-87 MG (A) and LN-229 (B) cells infected with lentivirus encoding control or cadherin-11 shRNA

    Techniques Used: Migration, In Vitro, Infection, shRNA

    Knockdown of cadherin-11 reduces growth factor-independent survival of glioma cells in vitro . U-87 MG or LN-229 glioma cells infected with lentivirus encoding control or cadherin-11 shRNA were plated at low density and allowed to form colonies under growth
    Figure Legend Snippet: Knockdown of cadherin-11 reduces growth factor-independent survival of glioma cells in vitro . U-87 MG or LN-229 glioma cells infected with lentivirus encoding control or cadherin-11 shRNA were plated at low density and allowed to form colonies under growth

    Techniques Used: In Vitro, Infection, shRNA

    Knockdown of cadherin-11 inhibits LN-229 glioma cell survival in a heterotopic xenograft in vivo . A, LN-229 cells expressing control or cadherin-11 shRNA and GFP were injected into the flank of athymic nude mice and imaged for bright field and GFP fluorescence
    Figure Legend Snippet: Knockdown of cadherin-11 inhibits LN-229 glioma cell survival in a heterotopic xenograft in vivo . A, LN-229 cells expressing control or cadherin-11 shRNA and GFP were injected into the flank of athymic nude mice and imaged for bright field and GFP fluorescence

    Techniques Used: In Vivo, Expressing, shRNA, Injection, Mouse Assay, Fluorescence

    Analysis of cadherin-11 expression in human GBM tumors, human GBM stem-like cells, glioma cell lines and shRNA mediated knockdown of cadherin-11. A, Cadherin-11 is highly expressed in human GBM tumors (GBM Center and GBM Edge) compared to normal human
    Figure Legend Snippet: Analysis of cadherin-11 expression in human GBM tumors, human GBM stem-like cells, glioma cell lines and shRNA mediated knockdown of cadherin-11. A, Cadherin-11 is highly expressed in human GBM tumors (GBM Center and GBM Edge) compared to normal human

    Techniques Used: Expressing, shRNA

    14) Product Images from "Genomic Locus Modulating IOP in the BXD RI Mouse Strains"

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200190

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Figure Legend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Techniques Used: Staining

    15) Product Images from "Genomic Locus Modulating IOP in the BXD RI Mouse Strains"

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200190

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Figure Legend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Techniques Used: Staining

    16) Product Images from "Genomic Locus Modulating IOP in the BXD RI Mouse Strains"

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200190

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Figure Legend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Techniques Used: Staining

    17) Product Images from "Lactic acid promotes metastatic niche formation in bone metastasis of colorectal cancer"

    Article Title: Lactic acid promotes metastatic niche formation in bone metastasis of colorectal cancer

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-020-00667-x

    LA induces the overexpression of Cadherin-11 in osteoclast precursors through PI3K-AKT-mTOR pathway. a KEGG pathway analysis of differentially regulated targets (left) and the expression of Cdh11 (right). b RT-PCR analysis showed the mRNA expression of Cadherin-11 in CD115(+) precursors treated by LA with/without each antagonist of PI3K-AKT pathway in vitro for 72 h. c Western blots analysis showed the protein level of Cadherin-11 in CD115(+) precursors after treated by LA as well as each antagonist of PI3K-AKT-mTOR pathway, respectively, and the quantification of relative intensity. d Flow cytometry analysis showed the percentage of Cadherin-11(+) cells in CD115(+) precursors at specified timepoints after injection of MC-38 cells. e After intratibially injecting MC-38 cells, LA with/without each antagonist of PI3K-AKT-mTOR pathway was injected. After 5 days, the percentage of Cadherin-11(+) cells in CD115(+) precursors was analyzed by flow cytometry, and the quantification of the percentage of Cadherin-11(+) precursors. * p
    Figure Legend Snippet: LA induces the overexpression of Cadherin-11 in osteoclast precursors through PI3K-AKT-mTOR pathway. a KEGG pathway analysis of differentially regulated targets (left) and the expression of Cdh11 (right). b RT-PCR analysis showed the mRNA expression of Cadherin-11 in CD115(+) precursors treated by LA with/without each antagonist of PI3K-AKT pathway in vitro for 72 h. c Western blots analysis showed the protein level of Cadherin-11 in CD115(+) precursors after treated by LA as well as each antagonist of PI3K-AKT-mTOR pathway, respectively, and the quantification of relative intensity. d Flow cytometry analysis showed the percentage of Cadherin-11(+) cells in CD115(+) precursors at specified timepoints after injection of MC-38 cells. e After intratibially injecting MC-38 cells, LA with/without each antagonist of PI3K-AKT-mTOR pathway was injected. After 5 days, the percentage of Cadherin-11(+) cells in CD115(+) precursors was analyzed by flow cytometry, and the quantification of the percentage of Cadherin-11(+) precursors. * p

    Techniques Used: Over Expression, Expressing, Reverse Transcription Polymerase Chain Reaction, In Vitro, Western Blot, Flow Cytometry, Injection

    The overexpression of Cadherin-11 enhances the adhesion ability of osteoclast precursors. a RT-PCR analysis tested the mRNA level of Cadherin-11 in CD115(+) precursors to verify the efficiency of Cadherin-11 siRNA. b Transwell assay showed the mobility of CD115(+) precursors after transfected with Cadherin-11 siRNA or negative control in the presence of LA or not for 12 h. c The quantification of Transwell assay in ( b ) by using absorbency analysis. d Immunofluorescence analysis showed Cadherin-11 expression in CD115(+) precursors after treated by LA for 72 h (left), the percentage of Cadherin-11 and CD115 dual-positive cells in total cells was calculated (middle) and the quantification of adhesive CD115(+) precursors (right). * p
    Figure Legend Snippet: The overexpression of Cadherin-11 enhances the adhesion ability of osteoclast precursors. a RT-PCR analysis tested the mRNA level of Cadherin-11 in CD115(+) precursors to verify the efficiency of Cadherin-11 siRNA. b Transwell assay showed the mobility of CD115(+) precursors after transfected with Cadherin-11 siRNA or negative control in the presence of LA or not for 12 h. c The quantification of Transwell assay in ( b ) by using absorbency analysis. d Immunofluorescence analysis showed Cadherin-11 expression in CD115(+) precursors after treated by LA for 72 h (left), the percentage of Cadherin-11 and CD115 dual-positive cells in total cells was calculated (middle) and the quantification of adhesive CD115(+) precursors (right). * p

    Techniques Used: Over Expression, Reverse Transcription Polymerase Chain Reaction, Transwell Assay, Transfection, Negative Control, Immunofluorescence, Expressing

    LA facilitates the fibrosis through upregulating the expression of Cadherin-11. a Go enrichment analysis showed pathways related to Cadherin-11 in LA-treated CD115(+) precursors. b RT-PCR analysis detected the mRNA levels of specific fibrotic markers in bone marrow at different timepoints after intratibially injection of MC-38 cells. c - g The primary CD115(+) precursors were isolated and transfected with Cadherin-11 siRNA or negative controls, then the cells were treated by LA for 72 h. The mRNA expression of each fibrotic marker was detected by RT-PCR. h After injecting MC-38 cells, LA was injected intraperitoneally for 5 days and Cadherin-11 siRNA was injected every 2 days. Then the primary CD115(+) precursors were isolated and fixed. Immunofluorescence analysis revealed the expression of Col5a2 in CD115(+) precursors sorted from mice in each group (left) and the quantification of percentage of Col5a2(+) cells (right) (Scale bar = 50 μm). * p
    Figure Legend Snippet: LA facilitates the fibrosis through upregulating the expression of Cadherin-11. a Go enrichment analysis showed pathways related to Cadherin-11 in LA-treated CD115(+) precursors. b RT-PCR analysis detected the mRNA levels of specific fibrotic markers in bone marrow at different timepoints after intratibially injection of MC-38 cells. c - g The primary CD115(+) precursors were isolated and transfected with Cadherin-11 siRNA or negative controls, then the cells were treated by LA for 72 h. The mRNA expression of each fibrotic marker was detected by RT-PCR. h After injecting MC-38 cells, LA was injected intraperitoneally for 5 days and Cadherin-11 siRNA was injected every 2 days. Then the primary CD115(+) precursors were isolated and fixed. Immunofluorescence analysis revealed the expression of Col5a2 in CD115(+) precursors sorted from mice in each group (left) and the quantification of percentage of Col5a2(+) cells (right) (Scale bar = 50 μm). * p

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Injection, Isolation, Transfection, Marker, Immunofluorescence, Mouse Assay

    18) Product Images from "Genomic Locus Modulating IOP in the BXD RI Mouse Strains"

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200190

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Figure Legend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Techniques Used: Staining

    19) Product Images from "Interactions between cadherin-11 and platelet-derived growth factor receptor-alpha signaling link cell adhesion and proliferation"

    Article Title: Interactions between cadherin-11 and platelet-derived growth factor receptor-alpha signaling link cell adhesion and proliferation

    Journal: Biochimica et biophysica acta. Molecular basis of disease

    doi: 10.1016/j.bbadis.2019.03.001

    Synovial fibroblast PDGFRα activated by cadherin-11. ( A ) RTK phosphorylation was compared in cell lysates made from serum-starved human synovial fibroblasts stimulated with or without cad11Fc for 10 minutes using a western blot array. ( B-C ) PDGFR phosphorylation after cad11Fc stimulation was confirmed using ELISA. ( B ) A representative experiment is shown demonstrating both phosphorylated (left y-axis) and total (right y-axis) PDGFR expression in serum-starved fibroblasts stimulated for 5 minutes with media alone, 40 μg/ml human IgG 1 isotype control, 40 μg/ml cad11Fc, 50 ng/ml PDGF-AA, 50 ng/ml PDGF-BB, or 10 ng/ml TNF-α. ( C ) Mean PDGFRα or PDGFRβ phosphorylation induced by cad11Fc was calculated as a percent of total PDGFRα or PDGFRβ receptor expression (n=8 experiments with five RA fibroblast lines). Comparison cad11Fc v. control: * p
    Figure Legend Snippet: Synovial fibroblast PDGFRα activated by cadherin-11. ( A ) RTK phosphorylation was compared in cell lysates made from serum-starved human synovial fibroblasts stimulated with or without cad11Fc for 10 minutes using a western blot array. ( B-C ) PDGFR phosphorylation after cad11Fc stimulation was confirmed using ELISA. ( B ) A representative experiment is shown demonstrating both phosphorylated (left y-axis) and total (right y-axis) PDGFR expression in serum-starved fibroblasts stimulated for 5 minutes with media alone, 40 μg/ml human IgG 1 isotype control, 40 μg/ml cad11Fc, 50 ng/ml PDGF-AA, 50 ng/ml PDGF-BB, or 10 ng/ml TNF-α. ( C ) Mean PDGFRα or PDGFRβ phosphorylation induced by cad11Fc was calculated as a percent of total PDGFRα or PDGFRβ receptor expression (n=8 experiments with five RA fibroblast lines). Comparison cad11Fc v. control: * p

    Techniques Used: Western Blot, Enzyme-linked Immunosorbent Assay, Expressing

    Cadherin-11 regulates proliferation through PDGFRα activation. ( A ) Synovial fibroblasts were pretreated with the multi-kinase inhibitor axitinib or vehicle control for one hour before stimulation by indicated methods. Proliferation was measured one day later by 3 H-thymidine incorporation (n= 6 experiments, three RA fibroblast lines). Comparison DMSO to axitinib-treated: *p=0.042, repeated measured one-way ANOVA. ( B ) PDGFRα and PDGFRβ gene expression was silenced by siRNA transfection before measuring proliferation after cad11Fc stimulation using 3 H-thymidine incorporation (n=6 experiments, five RA fibroblast lines). Comparison isotype to cad11Fc: **No siRNA p=0.028; PDGFRα siRNA p=0.83; ***PDGFRβ siRNA p=0.014, paired t-test. ( C ) PDGFRα or PDGFRβ surface expression was measured by flow cytometry in fibroblasts transfected with the indicated siRNA and percent expression of each receptor compared to control was calculated (n=4 RA fibroblasts).
    Figure Legend Snippet: Cadherin-11 regulates proliferation through PDGFRα activation. ( A ) Synovial fibroblasts were pretreated with the multi-kinase inhibitor axitinib or vehicle control for one hour before stimulation by indicated methods. Proliferation was measured one day later by 3 H-thymidine incorporation (n= 6 experiments, three RA fibroblast lines). Comparison DMSO to axitinib-treated: *p=0.042, repeated measured one-way ANOVA. ( B ) PDGFRα and PDGFRβ gene expression was silenced by siRNA transfection before measuring proliferation after cad11Fc stimulation using 3 H-thymidine incorporation (n=6 experiments, five RA fibroblast lines). Comparison isotype to cad11Fc: **No siRNA p=0.028; PDGFRα siRNA p=0.83; ***PDGFRβ siRNA p=0.014, paired t-test. ( C ) PDGFRα or PDGFRβ surface expression was measured by flow cytometry in fibroblasts transfected with the indicated siRNA and percent expression of each receptor compared to control was calculated (n=4 RA fibroblasts).

    Techniques Used: Activation Assay, Expressing, Transfection, Flow Cytometry

    PDGFRα mediates downstream Akt and MAPK activation by cadherin-11 signaling. ( A ) Serum-starved synovial fibroblasts were pretreated with the indicated inhibitors or vehicle control (DMSO) for 1 hour and then incubated overnight with or without 20 μg/ml cad11Fc or 50 ng/ml PDGF-BB. Proliferation was measured by 3 H-thymidine incorporation. The results are expressed as percent change in proliferation from baseline (media alone). The data summarizes five experiments with four RA fibroblast lines. Comparison to DMSO control: *p ≤0.017 and **p=0.025, paired t-test. ( B-C ) Synovial fibroblast PDGFRα or PDGFRβ expression was silenced by siRNA transfection. Lysates generated after a 15-minute stimulation with cad11Fc or isotype control antibody were analyzed for the indicated signaling molecules or loading controls. ( B ) A representative western blot experiment demonstrates changes in Akt, ERK, JNK, and p38 phosphorylation after cad11Fc stimulation in control, PDGFRα, and PDGFRβ siRNA silenced fibroblasts. Isotype and Cad11Fc lanes are from the same gels but are separated because non-relevant lanes were removed. ( C ) Pixel density for phosphorylation Akt and MAPKs bands was measured using Image J. Fold change in pixel density after cad11Fc stimulation was calculated by comparing the increase in pixel density in PDGFRα or PDGFRβ silenced cells with that in control siRNA treated cells (Experimental cad11Fc – Experimental isotype ) /(Control cad11Fc – Control isotype’ where experimental values used include control, PDGFRα, and PDGFRα siRNA pixel density). Data summarizes four independent experiments for pAkt, pERK, and pJNK and two independent experiments for p-p38. Comparison control to PDGFRα siRNA: *p=0.0009 and **p=0.038, paired t-test.
    Figure Legend Snippet: PDGFRα mediates downstream Akt and MAPK activation by cadherin-11 signaling. ( A ) Serum-starved synovial fibroblasts were pretreated with the indicated inhibitors or vehicle control (DMSO) for 1 hour and then incubated overnight with or without 20 μg/ml cad11Fc or 50 ng/ml PDGF-BB. Proliferation was measured by 3 H-thymidine incorporation. The results are expressed as percent change in proliferation from baseline (media alone). The data summarizes five experiments with four RA fibroblast lines. Comparison to DMSO control: *p ≤0.017 and **p=0.025, paired t-test. ( B-C ) Synovial fibroblast PDGFRα or PDGFRβ expression was silenced by siRNA transfection. Lysates generated after a 15-minute stimulation with cad11Fc or isotype control antibody were analyzed for the indicated signaling molecules or loading controls. ( B ) A representative western blot experiment demonstrates changes in Akt, ERK, JNK, and p38 phosphorylation after cad11Fc stimulation in control, PDGFRα, and PDGFRβ siRNA silenced fibroblasts. Isotype and Cad11Fc lanes are from the same gels but are separated because non-relevant lanes were removed. ( C ) Pixel density for phosphorylation Akt and MAPKs bands was measured using Image J. Fold change in pixel density after cad11Fc stimulation was calculated by comparing the increase in pixel density in PDGFRα or PDGFRβ silenced cells with that in control siRNA treated cells (Experimental cad11Fc – Experimental isotype ) /(Control cad11Fc – Control isotype’ where experimental values used include control, PDGFRα, and PDGFRα siRNA pixel density). Data summarizes four independent experiments for pAkt, pERK, and pJNK and two independent experiments for p-p38. Comparison control to PDGFRα siRNA: *p=0.0009 and **p=0.038, paired t-test.

    Techniques Used: Activation Assay, Incubation, Expressing, Transfection, Generated, Western Blot

    20) Product Images from "MicroRNA-145 suppresses cell invasion and metastasis by directly targeting mucin 1"

    Article Title: MicroRNA-145 suppresses cell invasion and metastasis by directly targeting mucin 1

    Journal: Cancer research

    doi: 10.1158/0008-5472.CAN-09-2021

    Effect of miR-145 on β-catenin and cadherin 11
    Figure Legend Snippet: Effect of miR-145 on β-catenin and cadherin 11

    Techniques Used:

    Suppression of MUC1 by RNAi reduces levels of β-catenin, cyclin D1 and cadherin 11 as well as invasiveness
    Figure Legend Snippet: Suppression of MUC1 by RNAi reduces levels of β-catenin, cyclin D1 and cadherin 11 as well as invasiveness

    Techniques Used:

    21) Product Images from "Regulation of cadherin expression in the chicken neural crest by the Wnt/?-catenin signaling pathway"

    Article Title: Regulation of cadherin expression in the chicken neural crest by the Wnt/?-catenin signaling pathway

    Journal: Cell Adhesion & Migration

    doi: 10.4161/cam.4.3.12138

    Cadherin-7 and cadherin-11 expression is upregulated by Wnt3a in neural crest cells. Cadherin-7, cadherin-11 and Sox10 expression in neural crest cells after 24 h of culture with Wnt-conditioned medium (dark bars) or control-conditioned medium (light
    Figure Legend Snippet: Cadherin-7 and cadherin-11 expression is upregulated by Wnt3a in neural crest cells. Cadherin-7, cadherin-11 and Sox10 expression in neural crest cells after 24 h of culture with Wnt-conditioned medium (dark bars) or control-conditioned medium (light

    Techniques Used: Expressing

    Analysis of cadherin-11 expression in neural crest cell culture by real-time RT-PCR and immunostaining. (A) Cadherin-11 transcript is detected by real-time RT-PCR in both the neuroepithelium (NT) and the neural crest (NC) cells in 24 h neural tube explant
    Figure Legend Snippet: Analysis of cadherin-11 expression in neural crest cell culture by real-time RT-PCR and immunostaining. (A) Cadherin-11 transcript is detected by real-time RT-PCR in both the neuroepithelium (NT) and the neural crest (NC) cells in 24 h neural tube explant

    Techniques Used: Expressing, Cell Culture, Quantitative RT-PCR, Immunostaining

    Wnt increases the cadherin-7 and cadherin-11 protein at cell-cell interfaces of the neural crest.
    Figure Legend Snippet: Wnt increases the cadherin-7 and cadherin-11 protein at cell-cell interfaces of the neural crest.

    Techniques Used:

    Cadherin-7 and cadherin-11 protein is upregulated by Wnt3a at cell-cell interfaces in neural crest cells. Neural crest cells cultured with Wnt-conditioned medium (A and C) or control-conditioned medium (B and D) were immunostained for cadherin-7 (A and
    Figure Legend Snippet: Cadherin-7 and cadherin-11 protein is upregulated by Wnt3a at cell-cell interfaces in neural crest cells. Neural crest cells cultured with Wnt-conditioned medium (A and C) or control-conditioned medium (B and D) were immunostained for cadherin-7 (A and

    Techniques Used: Cell Culture

    Cadherin-11 expression in the chicken embryo. (A–D) At the hindlimb level of a st 17 embryo, cadherin-11 (A–C, Cy3, red) is expressed in the mesenchyme (white asterisks in B) and in migrating neural crest cells (white arrows indicating
    Figure Legend Snippet: Cadherin-11 expression in the chicken embryo. (A–D) At the hindlimb level of a st 17 embryo, cadherin-11 (A–C, Cy3, red) is expressed in the mesenchyme (white asterisks in B) and in migrating neural crest cells (white arrows indicating

    Techniques Used: Expressing

    22) Product Images from "Interactions between cadherin-11 and platelet-derived growth factor receptor-alpha signaling link cell adhesion and proliferation"

    Article Title: Interactions between cadherin-11 and platelet-derived growth factor receptor-alpha signaling link cell adhesion and proliferation

    Journal: Biochimica et biophysica acta. Molecular basis of disease

    doi: 10.1016/j.bbadis.2019.03.001

    Synovial fibroblast PDGFRα activated by cadherin-11. ( A ) RTK phosphorylation was compared in cell lysates made from serum-starved human synovial fibroblasts stimulated with or without cad11Fc for 10 minutes using a western blot array. ( B-C ) PDGFR phosphorylation after cad11Fc stimulation was confirmed using ELISA. ( B ) A representative experiment is shown demonstrating both phosphorylated (left y-axis) and total (right y-axis) PDGFR expression in serum-starved fibroblasts stimulated for 5 minutes with media alone, 40 μg/ml human IgG 1 isotype control, 40 μg/ml cad11Fc, 50 ng/ml PDGF-AA, 50 ng/ml PDGF-BB, or 10 ng/ml TNF-α. ( C ) Mean PDGFRα or PDGFRβ phosphorylation induced by cad11Fc was calculated as a percent of total PDGFRα or PDGFRβ receptor expression (n=8 experiments with five RA fibroblast lines). Comparison cad11Fc v. control: * p
    Figure Legend Snippet: Synovial fibroblast PDGFRα activated by cadherin-11. ( A ) RTK phosphorylation was compared in cell lysates made from serum-starved human synovial fibroblasts stimulated with or without cad11Fc for 10 minutes using a western blot array. ( B-C ) PDGFR phosphorylation after cad11Fc stimulation was confirmed using ELISA. ( B ) A representative experiment is shown demonstrating both phosphorylated (left y-axis) and total (right y-axis) PDGFR expression in serum-starved fibroblasts stimulated for 5 minutes with media alone, 40 μg/ml human IgG 1 isotype control, 40 μg/ml cad11Fc, 50 ng/ml PDGF-AA, 50 ng/ml PDGF-BB, or 10 ng/ml TNF-α. ( C ) Mean PDGFRα or PDGFRβ phosphorylation induced by cad11Fc was calculated as a percent of total PDGFRα or PDGFRβ receptor expression (n=8 experiments with five RA fibroblast lines). Comparison cad11Fc v. control: * p

    Techniques Used: Western Blot, Enzyme-linked Immunosorbent Assay, Expressing

    Cadherin-11 regulates proliferation through PDGFRα activation. ( A ) Synovial fibroblasts were pretreated with the multi-kinase inhibitor axitinib or vehicle control for one hour before stimulation by indicated methods. Proliferation was measured one day later by 3 H-thymidine incorporation (n= 6 experiments, three RA fibroblast lines). Comparison DMSO to axitinib-treated: *p=0.042, repeated measured one-way ANOVA. ( B ) PDGFRα and PDGFRβ gene expression was silenced by siRNA transfection before measuring proliferation after cad11Fc stimulation using 3 H-thymidine incorporation (n=6 experiments, five RA fibroblast lines). Comparison isotype to cad11Fc: **No siRNA p=0.028; PDGFRα siRNA p=0.83; ***PDGFRβ siRNA p=0.014, paired t-test. ( C ) PDGFRα or PDGFRβ surface expression was measured by flow cytometry in fibroblasts transfected with the indicated siRNA and percent expression of each receptor compared to control was calculated (n=4 RA fibroblasts).
    Figure Legend Snippet: Cadherin-11 regulates proliferation through PDGFRα activation. ( A ) Synovial fibroblasts were pretreated with the multi-kinase inhibitor axitinib or vehicle control for one hour before stimulation by indicated methods. Proliferation was measured one day later by 3 H-thymidine incorporation (n= 6 experiments, three RA fibroblast lines). Comparison DMSO to axitinib-treated: *p=0.042, repeated measured one-way ANOVA. ( B ) PDGFRα and PDGFRβ gene expression was silenced by siRNA transfection before measuring proliferation after cad11Fc stimulation using 3 H-thymidine incorporation (n=6 experiments, five RA fibroblast lines). Comparison isotype to cad11Fc: **No siRNA p=0.028; PDGFRα siRNA p=0.83; ***PDGFRβ siRNA p=0.014, paired t-test. ( C ) PDGFRα or PDGFRβ surface expression was measured by flow cytometry in fibroblasts transfected with the indicated siRNA and percent expression of each receptor compared to control was calculated (n=4 RA fibroblasts).

    Techniques Used: Activation Assay, Expressing, Transfection, Flow Cytometry

    PDGFRα mediates downstream Akt and MAPK activation by cadherin-11 signaling. ( A ) Serum-starved synovial fibroblasts were pretreated with the indicated inhibitors or vehicle control (DMSO) for 1 hour and then incubated overnight with or without 20 μg/ml cad11Fc or 50 ng/ml PDGF-BB. Proliferation was measured by 3 H-thymidine incorporation. The results are expressed as percent change in proliferation from baseline (media alone). The data summarizes five experiments with four RA fibroblast lines. Comparison to DMSO control: *p ≤0.017 and **p=0.025, paired t-test. ( B-C ) Synovial fibroblast PDGFRα or PDGFRβ expression was silenced by siRNA transfection. Lysates generated after a 15-minute stimulation with cad11Fc or isotype control antibody were analyzed for the indicated signaling molecules or loading controls. ( B ) A representative western blot experiment demonstrates changes in Akt, ERK, JNK, and p38 phosphorylation after cad11Fc stimulation in control, PDGFRα, and PDGFRβ siRNA silenced fibroblasts. Isotype and Cad11Fc lanes are from the same gels but are separated because non-relevant lanes were removed. ( C ) Pixel density for phosphorylation Akt and MAPKs bands was measured using Image J. Fold change in pixel density after cad11Fc stimulation was calculated by comparing the increase in pixel density in PDGFRα or PDGFRβ silenced cells with that in control siRNA treated cells (Experimental cad11Fc – Experimental isotype ) /(Control cad11Fc – Control isotype’ where experimental values used include control, PDGFRα, and PDGFRα siRNA pixel density). Data summarizes four independent experiments for pAkt, pERK, and pJNK and two independent experiments for p-p38. Comparison control to PDGFRα siRNA: *p=0.0009 and **p=0.038, paired t-test.
    Figure Legend Snippet: PDGFRα mediates downstream Akt and MAPK activation by cadherin-11 signaling. ( A ) Serum-starved synovial fibroblasts were pretreated with the indicated inhibitors or vehicle control (DMSO) for 1 hour and then incubated overnight with or without 20 μg/ml cad11Fc or 50 ng/ml PDGF-BB. Proliferation was measured by 3 H-thymidine incorporation. The results are expressed as percent change in proliferation from baseline (media alone). The data summarizes five experiments with four RA fibroblast lines. Comparison to DMSO control: *p ≤0.017 and **p=0.025, paired t-test. ( B-C ) Synovial fibroblast PDGFRα or PDGFRβ expression was silenced by siRNA transfection. Lysates generated after a 15-minute stimulation with cad11Fc or isotype control antibody were analyzed for the indicated signaling molecules or loading controls. ( B ) A representative western blot experiment demonstrates changes in Akt, ERK, JNK, and p38 phosphorylation after cad11Fc stimulation in control, PDGFRα, and PDGFRβ siRNA silenced fibroblasts. Isotype and Cad11Fc lanes are from the same gels but are separated because non-relevant lanes were removed. ( C ) Pixel density for phosphorylation Akt and MAPKs bands was measured using Image J. Fold change in pixel density after cad11Fc stimulation was calculated by comparing the increase in pixel density in PDGFRα or PDGFRβ silenced cells with that in control siRNA treated cells (Experimental cad11Fc – Experimental isotype ) /(Control cad11Fc – Control isotype’ where experimental values used include control, PDGFRα, and PDGFRα siRNA pixel density). Data summarizes four independent experiments for pAkt, pERK, and pJNK and two independent experiments for p-p38. Comparison control to PDGFRα siRNA: *p=0.0009 and **p=0.038, paired t-test.

    Techniques Used: Activation Assay, Incubation, Expressing, Transfection, Generated, Western Blot

    23) Product Images from "Genomic Locus Modulating IOP in the BXD RI Mouse Strains"

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200190

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Figure Legend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Techniques Used: Staining

    24) Product Images from "Androgen Depletion Upregulates Cadherin-11 Expression in Prostate Cancer"

    Article Title: Androgen Depletion Upregulates Cadherin-11 Expression in Prostate Cancer

    Journal: The Journal of pathology

    doi: 10.1002/path.2687

    Expression of cadherin-11 and AR in PCa cells. (a) The levels of cadherin-11 and AR transcripts were examined by semi-quantitative RT-PCR. GAPDH was used as a loading control. (b) The protein levels of cadherin-11 and AR in the PCa cell lines as indicated
    Figure Legend Snippet: Expression of cadherin-11 and AR in PCa cells. (a) The levels of cadherin-11 and AR transcripts were examined by semi-quantitative RT-PCR. GAPDH was used as a loading control. (b) The protein levels of cadherin-11 and AR in the PCa cell lines as indicated

    Techniques Used: Expressing, Quantitative RT-PCR

    Expression of cadherin-11 in MDA-PCa-2b tumors before and after castration. Tumors collected before castration, one week post-castration, two weeks post-castration, and 15 weeks post-castration were immunostained with a goat anti-cadherin-11 polyclonal
    Figure Legend Snippet: Expression of cadherin-11 in MDA-PCa-2b tumors before and after castration. Tumors collected before castration, one week post-castration, two weeks post-castration, and 15 weeks post-castration were immunostained with a goat anti-cadherin-11 polyclonal

    Techniques Used: Expressing, Multiple Displacement Amplification

    Cadherin-11 Expression in Recurrent Tumors After Androgen-Deprivation Therapy
    Figure Legend Snippet: Cadherin-11 Expression in Recurrent Tumors After Androgen-Deprivation Therapy

    Techniques Used: Expressing

    Induction of cadherin-11 expression by inactivation of AR activity. (a) VCaP cells were cultured in 10% fetal bovine serum (FBS), charcoal-stripped FBS (cFBS), or cFBS supplemented with 1nM DHT for 72 h, and cadherin-11 transcripts were detected by real-time
    Figure Legend Snippet: Induction of cadherin-11 expression by inactivation of AR activity. (a) VCaP cells were cultured in 10% fetal bovine serum (FBS), charcoal-stripped FBS (cFBS), or cFBS supplemented with 1nM DHT for 72 h, and cadherin-11 transcripts were detected by real-time

    Techniques Used: Expressing, Activity Assay, Cell Culture

    Suppression of cadherin-11 expression by re-expressing AR in PC3 and PC3-mm2 cells. (a) PC3-AR(A2) cells grown in charcoal-stripped fetal bovine serum or treated with DHT or R1881 at the concentrations as indicated. RNAs were prepared and cadherin-11
    Figure Legend Snippet: Suppression of cadherin-11 expression by re-expressing AR in PC3 and PC3-mm2 cells. (a) PC3-AR(A2) cells grown in charcoal-stripped fetal bovine serum or treated with DHT or R1881 at the concentrations as indicated. RNAs were prepared and cadherin-11

    Techniques Used: Expressing

    Promoter activity of human cadherin-11. (a) Genomic organization of the human cadherin-11 gene. (b) Cloning of a 2-kb fragment of the cadherin-11 promoter from PC-3 DNA. (Note: the predicted sizes of introns and exons are not drawn to scale.) (c) Transcriptional
    Figure Legend Snippet: Promoter activity of human cadherin-11. (a) Genomic organization of the human cadherin-11 gene. (b) Cloning of a 2-kb fragment of the cadherin-11 promoter from PC-3 DNA. (Note: the predicted sizes of introns and exons are not drawn to scale.) (c) Transcriptional

    Techniques Used: Activity Assay, Clone Assay

    Cadherin-11 expression in human prostate tumors from patients with androgen-dependent and androgen-independent disease status. The immunohistochemical staining patterns of three representative tumors from each group are shown. The majority of androgen-independent
    Figure Legend Snippet: Cadherin-11 expression in human prostate tumors from patients with androgen-dependent and androgen-independent disease status. The immunohistochemical staining patterns of three representative tumors from each group are shown. The majority of androgen-independent

    Techniques Used: Expressing, Immunohistochemistry, Staining

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  • 93
    Thermo Fisher cdh 11 antibody
    Fresh and cryopreserved UCT expresses <t>CDH-11</t> mRNA. (A) Fresh and (B) frozen UCT from 4 different donors were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager.
    Cdh 11 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cadherin 11
    The distribution of <t>cadherin</t> 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Cadherin 11, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp cdh11 mm00515462 m1
    pRb deletion leads to abnormal cadherin expression. ( A ) Immunoblot of confluent cultures showing decreased levels of <t>OB-cadherin</t> (top panel) and β-catenin (bottom panel) and increased levels of N-cadherin (middle panel) in pRb-deficient cells relative to pRb-expressing controls. In the β-catenin panel, the top band corresponds to β-catenin while the lower bands are irrelevant background bands. Representative blots of at least 3 independent experiments are shown. Equal loading of the lanes is shown by GAPDH levels for each individual immunoblot. ( B ) qRT-PCR showing levels of OB-cadherin, N-cadherin, and β-catenin mRNAs in pRb-deficient osteoblasts relative to pRb-expressing osteoblasts (control bar). Each bar represents the average of at least 3 independent experiments ± SEM. Values were normalized against GAPDH. **, P
    Gene Exp Cdh11 Mm00515462 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fresh and cryopreserved UCT expresses CDH-11 mRNA. (A) Fresh and (B) frozen UCT from 4 different donors were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager.

    Journal: International Journal of Stem Cells

    Article Title: CD73, CD90, CD105 and Cadherin-11 RT-PCR Screening for Mesenchymal Stem Cells from Cryopreserved Human Cord Tissue

    doi: 10.15283/ijsc17015

    Figure Lengend Snippet: Fresh and cryopreserved UCT expresses CDH-11 mRNA. (A) Fresh and (B) frozen UCT from 4 different donors were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager.

    Article Snippet: CDH-11 antibody (Life Technologies, Carlsbad, CA), horseradish peroxidase-conjugated anti-rabbit IgG or anti-mouse IgG and ECL reagents were acquired from Thermo Fisher Scientific (Waltham, MA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Agarose Gel Electrophoresis

    MSC expansion from cryopreserved UCT expresses CDH-11. MSCs expanded from (A) fresh and (B) cryopreserved UCT sections were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager. (C) MSCs were processed for protein, denatured in loading buffer containing SDS, resolved on a 4~20% pre-cast gel and transferred onto nitrocellulose membranes. Membranes were probed with CDH-11 or GAPDH antibodies, visualized by ECL and captured on an imager.

    Journal: International Journal of Stem Cells

    Article Title: CD73, CD90, CD105 and Cadherin-11 RT-PCR Screening for Mesenchymal Stem Cells from Cryopreserved Human Cord Tissue

    doi: 10.15283/ijsc17015

    Figure Lengend Snippet: MSC expansion from cryopreserved UCT expresses CDH-11. MSCs expanded from (A) fresh and (B) cryopreserved UCT sections were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager. (C) MSCs were processed for protein, denatured in loading buffer containing SDS, resolved on a 4~20% pre-cast gel and transferred onto nitrocellulose membranes. Membranes were probed with CDH-11 or GAPDH antibodies, visualized by ECL and captured on an imager.

    Article Snippet: CDH-11 antibody (Life Technologies, Carlsbad, CA), horseradish peroxidase-conjugated anti-rabbit IgG or anti-mouse IgG and ECL reagents were acquired from Thermo Fisher Scientific (Waltham, MA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Agarose Gel Electrophoresis

    LA induces the overexpression of Cadherin-11 in osteoclast precursors through PI3K-AKT-mTOR pathway. a KEGG pathway analysis of differentially regulated targets (left) and the expression of Cdh11 (right). b RT-PCR analysis showed the mRNA expression of Cadherin-11 in CD115(+) precursors treated by LA with/without each antagonist of PI3K-AKT pathway in vitro for 72 h. c Western blots analysis showed the protein level of Cadherin-11 in CD115(+) precursors after treated by LA as well as each antagonist of PI3K-AKT-mTOR pathway, respectively, and the quantification of relative intensity. d Flow cytometry analysis showed the percentage of Cadherin-11(+) cells in CD115(+) precursors at specified timepoints after injection of MC-38 cells. e After intratibially injecting MC-38 cells, LA with/without each antagonist of PI3K-AKT-mTOR pathway was injected. After 5 days, the percentage of Cadherin-11(+) cells in CD115(+) precursors was analyzed by flow cytometry, and the quantification of the percentage of Cadherin-11(+) precursors. * p

    Journal: Cell Communication and Signaling : CCS

    Article Title: Lactic acid promotes metastatic niche formation in bone metastasis of colorectal cancer

    doi: 10.1186/s12964-020-00667-x

    Figure Lengend Snippet: LA induces the overexpression of Cadherin-11 in osteoclast precursors through PI3K-AKT-mTOR pathway. a KEGG pathway analysis of differentially regulated targets (left) and the expression of Cdh11 (right). b RT-PCR analysis showed the mRNA expression of Cadherin-11 in CD115(+) precursors treated by LA with/without each antagonist of PI3K-AKT pathway in vitro for 72 h. c Western blots analysis showed the protein level of Cadherin-11 in CD115(+) precursors after treated by LA as well as each antagonist of PI3K-AKT-mTOR pathway, respectively, and the quantification of relative intensity. d Flow cytometry analysis showed the percentage of Cadherin-11(+) cells in CD115(+) precursors at specified timepoints after injection of MC-38 cells. e After intratibially injecting MC-38 cells, LA with/without each antagonist of PI3K-AKT-mTOR pathway was injected. After 5 days, the percentage of Cadherin-11(+) cells in CD115(+) precursors was analyzed by flow cytometry, and the quantification of the percentage of Cadherin-11(+) precursors. * p

    Article Snippet: A CD115 antibody diluted 1:100 (Novus Biologicals), Ki67 antibody diluted 1:100 (Abcam), TRITC-conjugated phalloidin diluted 1:200 (Yeason), cadherin-11 antibody diluted 1:50 (Invitrogen), and collagen 5 antibody diluted 1:100 (Abcam) were used.

    Techniques: Over Expression, Expressing, Reverse Transcription Polymerase Chain Reaction, In Vitro, Western Blot, Flow Cytometry, Injection

    The overexpression of Cadherin-11 enhances the adhesion ability of osteoclast precursors. a RT-PCR analysis tested the mRNA level of Cadherin-11 in CD115(+) precursors to verify the efficiency of Cadherin-11 siRNA. b Transwell assay showed the mobility of CD115(+) precursors after transfected with Cadherin-11 siRNA or negative control in the presence of LA or not for 12 h. c The quantification of Transwell assay in ( b ) by using absorbency analysis. d Immunofluorescence analysis showed Cadherin-11 expression in CD115(+) precursors after treated by LA for 72 h (left), the percentage of Cadherin-11 and CD115 dual-positive cells in total cells was calculated (middle) and the quantification of adhesive CD115(+) precursors (right). * p

    Journal: Cell Communication and Signaling : CCS

    Article Title: Lactic acid promotes metastatic niche formation in bone metastasis of colorectal cancer

    doi: 10.1186/s12964-020-00667-x

    Figure Lengend Snippet: The overexpression of Cadherin-11 enhances the adhesion ability of osteoclast precursors. a RT-PCR analysis tested the mRNA level of Cadherin-11 in CD115(+) precursors to verify the efficiency of Cadherin-11 siRNA. b Transwell assay showed the mobility of CD115(+) precursors after transfected with Cadherin-11 siRNA or negative control in the presence of LA or not for 12 h. c The quantification of Transwell assay in ( b ) by using absorbency analysis. d Immunofluorescence analysis showed Cadherin-11 expression in CD115(+) precursors after treated by LA for 72 h (left), the percentage of Cadherin-11 and CD115 dual-positive cells in total cells was calculated (middle) and the quantification of adhesive CD115(+) precursors (right). * p

    Article Snippet: A CD115 antibody diluted 1:100 (Novus Biologicals), Ki67 antibody diluted 1:100 (Abcam), TRITC-conjugated phalloidin diluted 1:200 (Yeason), cadherin-11 antibody diluted 1:50 (Invitrogen), and collagen 5 antibody diluted 1:100 (Abcam) were used.

    Techniques: Over Expression, Reverse Transcription Polymerase Chain Reaction, Transwell Assay, Transfection, Negative Control, Immunofluorescence, Expressing

    LA facilitates the fibrosis through upregulating the expression of Cadherin-11. a Go enrichment analysis showed pathways related to Cadherin-11 in LA-treated CD115(+) precursors. b RT-PCR analysis detected the mRNA levels of specific fibrotic markers in bone marrow at different timepoints after intratibially injection of MC-38 cells. c - g The primary CD115(+) precursors were isolated and transfected with Cadherin-11 siRNA or negative controls, then the cells were treated by LA for 72 h. The mRNA expression of each fibrotic marker was detected by RT-PCR. h After injecting MC-38 cells, LA was injected intraperitoneally for 5 days and Cadherin-11 siRNA was injected every 2 days. Then the primary CD115(+) precursors were isolated and fixed. Immunofluorescence analysis revealed the expression of Col5a2 in CD115(+) precursors sorted from mice in each group (left) and the quantification of percentage of Col5a2(+) cells (right) (Scale bar = 50 μm). * p

    Journal: Cell Communication and Signaling : CCS

    Article Title: Lactic acid promotes metastatic niche formation in bone metastasis of colorectal cancer

    doi: 10.1186/s12964-020-00667-x

    Figure Lengend Snippet: LA facilitates the fibrosis through upregulating the expression of Cadherin-11. a Go enrichment analysis showed pathways related to Cadherin-11 in LA-treated CD115(+) precursors. b RT-PCR analysis detected the mRNA levels of specific fibrotic markers in bone marrow at different timepoints after intratibially injection of MC-38 cells. c - g The primary CD115(+) precursors were isolated and transfected with Cadherin-11 siRNA or negative controls, then the cells were treated by LA for 72 h. The mRNA expression of each fibrotic marker was detected by RT-PCR. h After injecting MC-38 cells, LA was injected intraperitoneally for 5 days and Cadherin-11 siRNA was injected every 2 days. Then the primary CD115(+) precursors were isolated and fixed. Immunofluorescence analysis revealed the expression of Col5a2 in CD115(+) precursors sorted from mice in each group (left) and the quantification of percentage of Col5a2(+) cells (right) (Scale bar = 50 μm). * p

    Article Snippet: A CD115 antibody diluted 1:100 (Novus Biologicals), Ki67 antibody diluted 1:100 (Abcam), TRITC-conjugated phalloidin diluted 1:200 (Yeason), cadherin-11 antibody diluted 1:50 (Invitrogen), and collagen 5 antibody diluted 1:100 (Abcam) were used.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Injection, Isolation, Transfection, Marker, Immunofluorescence, Mouse Assay

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Journal: G3: Genes|Genomes|Genetics

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    doi: 10.1534/g3.118.200190

    Figure Lengend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Article Snippet: The sections were incubated in primary antibodies (1:500) against Cadherin 11 (Thermofisher, Cat. #71-7600, Waltham, MA) overnight at 4°.

    Techniques: Staining

    pRb deletion leads to abnormal cadherin expression. ( A ) Immunoblot of confluent cultures showing decreased levels of OB-cadherin (top panel) and β-catenin (bottom panel) and increased levels of N-cadherin (middle panel) in pRb-deficient cells relative to pRb-expressing controls. In the β-catenin panel, the top band corresponds to β-catenin while the lower bands are irrelevant background bands. Representative blots of at least 3 independent experiments are shown. Equal loading of the lanes is shown by GAPDH levels for each individual immunoblot. ( B ) qRT-PCR showing levels of OB-cadherin, N-cadherin, and β-catenin mRNAs in pRb-deficient osteoblasts relative to pRb-expressing osteoblasts (control bar). Each bar represents the average of at least 3 independent experiments ± SEM. Values were normalized against GAPDH. **, P

    Journal: PLoS ONE

    Article Title: A Role for the Retinoblastoma Protein As a Regulator of Mouse Osteoblast Cell Adhesion: Implications for Osteogenesis and Osteosarcoma Formation

    doi: 10.1371/journal.pone.0013954

    Figure Lengend Snippet: pRb deletion leads to abnormal cadherin expression. ( A ) Immunoblot of confluent cultures showing decreased levels of OB-cadherin (top panel) and β-catenin (bottom panel) and increased levels of N-cadherin (middle panel) in pRb-deficient cells relative to pRb-expressing controls. In the β-catenin panel, the top band corresponds to β-catenin while the lower bands are irrelevant background bands. Representative blots of at least 3 independent experiments are shown. Equal loading of the lanes is shown by GAPDH levels for each individual immunoblot. ( B ) qRT-PCR showing levels of OB-cadherin, N-cadherin, and β-catenin mRNAs in pRb-deficient osteoblasts relative to pRb-expressing osteoblasts (control bar). Each bar represents the average of at least 3 independent experiments ± SEM. Values were normalized against GAPDH. **, P

    Article Snippet: TaqMan® assays used for these studies were: Pak1 (Mm0044612_m1), Rac1 (Mm00488847_m1), OB-cadherin (Mm00515462_m1), N-cadherin (Mm00483213_m1), β-catenin (Mm00499427_s1), and GAPDH (Mm00483213_m1).

    Techniques: Expressing, Quantitative RT-PCR