caco 2  (ATCC)

Journal: Poultry Science

Article Title: Houttuynia cordata extract protects against Salmonella infection by targeting type III secretion system 1

doi: 10.1016/j.psj.2026.106937

Figure Lengend Snippet: HCE suppresses the adhesion and invasion of Salmonella in Caco-2 cells. A CCK-8 assay (A) was conducted to evaluate the cytotoxicity of HCE in Caco-2 cells. The effects of HCE on the adhesion (B, D) and invasion (C, E) of ST (B-C) and SP (D-E) to Caco-2 cells were examined at an MOI of 100 (n = 5). Immunofluorescence microscopy was performed to visualize the inhibitory effect of HCE (150 µg/mL) on the invasion of ST (F) and SP (G) in Caco-2 cells. Caco-2 cells infected with the ST Δ invA strain, which was used as a negative control because of its T3SS-1 deficiency showed significantly reduced invasion. ST, Salmonella Typhimurium. SP, Salmonella Pullorum . HCE, Houttuynia cordata extract.

Article Snippet: Caco-2 cells (American Type Culture Collection, ATCC, USA) were cultured in DMEM and seeded into culture plates.

Techniques: CCK-8 Assay, Immunofluorescence, Microscopy, Infection, Negative Control

Journal: Bioactive Materials

Article Title: Harnessing the gut–immune–joint axis: Oral microalgae-based thermoresponsive microspheres enhance intra-articular therapy for rheumatoid arthritis

doi: 10.1016/j.bioactmat.2026.01.037

Figure Lengend Snippet: Evaluation of the protective effects of CG@GelMA on FSN-induced intestinal epithelial barrier disruption in vitro . (A) Immunofluorescence staining of Claudin-1 in Caco-2 monolayers under different treatments. Scale bar: 100 μm. (B) Relative fluorescence intensity of Claudin-1. (C) Immunofluorescence staining of Occludin in Caco-2 monolayers. Scale bar: 100 μm. (D) Relative fluorescence intensity of Occludin. (E) Immunofluorescence staining of ZO-1 in Caco-2 monolayers. Scale bar: 100 μm. (F) Relative fluorescence intensity of ZO-1. (G) Schematic diagram of the Caco-2/RAW 264.7 Transwell co-culture system. (H) Relative TEER of Caco-2 cell monolayers after different treatments. (I) Relative fluorescence intensity of FD4 across Caco-2 monolayers under different treatments. Data are presented as means ± SD. Statistical significance: ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: RAW 264.7 macrophages (Procell, Wuhan, China), Caco-2 intestinal epithelial cells (Pricella, Wuhan, China), and IEC-6 small intestinal epithelial cells (ATCC, USA) were maintained in high-glucose DMEM supplemented with 10 % fetal bovine serum (AiTing, Hangzhou, China) and 1 % penicillin-streptomycin (Gibco, USA), with the medium for IEC-6 cells additionally containing 0.1 U/mL human insulin.

Techniques: Disruption, In Vitro, Immunofluorescence, Staining, Fluorescence, Co-Culture Assay