Journal: Molecular Neurobiology
Article Title: Involvement of the Voltage-Gated Calcium Channels L- P/Q- and N-Types in Synapse Elimination During Neuromuscular Junction Development
Figure Lengend Snippet: a Representative confocal image of a nerve terminal arborization. Singly, dually, and innervated by three or more axons NMJs from YFP muscles and also images of the morphologic maturation (S1, the most inmature, and S4, almost fully differentiated, stages) of the postsynaptic clusters from P9 mice. The bar indicates 10 μm. b Confocal immunofluorescence location of α 1D L-, N-, and P/Q-type voltage-dependent calcium channels (VDCCs) at the NMJ. Triple labeling of VDCCs (green fluorescence) with syntaxin (blue fluorescence) and nAChR-α-bungarotoxin (red fluorescence) in merge images. Figure shows the presence of α 1D L-, N-, and P/Q-type-VDCC (in green) in the nerve terminal of P9 Levator auris longus (LAL) muscle endplates. The bar indicates 10 μm
Article Snippet: Muscles were incubated overnight at 4 °C with anti-CaV 1.3 (CACNA1D) antibody voltage-dependent L-type calcium channel subunit α1D (1/100; ACC-005, Alomone Labs, Jerusalem, Israel); anti-CaV 2.1 (CACNA1A) antibody voltage-dependent P/Q-type calcium channel subunit α1A (1/100; ACC-001, Alomone Labs, Jerusalem, Israel); anti-CaV 2.2 (CACNA1B) antibody voltage-dependent N-type calcium channel subunit α1B (1/100; ACC1-002, Alomone Labs, Jerusalem, Israel), and anti-mouse syntaxin (1/1000, S066, Sigma, St Louis, MO, USA).
Techniques: Mouse Assay, Immunofluorescence, Labeling, Fluorescence