Structured Review

Millipore cacl2
Effect of Divalent Crosslinks in ASC Microbead on Growth Factor mRNA Levels and Production. (A) mRNA levels and (B) growth factor secretion/DNA content on day 7 from ASC microbeads crosslinked in <t>CaCl2</t> Ca++) or BaCl2 (Ba++), cultured in growth medium and normalized to ASCs on tissue culture polystyrene (TCPS). (C) Growth factor retained within microbeads normalized to DNA content (n = 6 samples, mean±SE, *p
Cacl2, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Adipose Stem Cell Microbeads as Production Sources for Chondrogenic Growth Factors"

Article Title: Adipose Stem Cell Microbeads as Production Sources for Chondrogenic Growth Factors

Journal: Journal of Stem Cells & Regenerative Medicine

doi:

Effect of Divalent Crosslinks in ASC Microbead on Growth Factor mRNA Levels and Production. (A) mRNA levels and (B) growth factor secretion/DNA content on day 7 from ASC microbeads crosslinked in CaCl2 Ca++) or BaCl2 (Ba++), cultured in growth medium and normalized to ASCs on tissue culture polystyrene (TCPS). (C) Growth factor retained within microbeads normalized to DNA content (n = 6 samples, mean±SE, *p
Figure Legend Snippet: Effect of Divalent Crosslinks in ASC Microbead on Growth Factor mRNA Levels and Production. (A) mRNA levels and (B) growth factor secretion/DNA content on day 7 from ASC microbeads crosslinked in CaCl2 Ca++) or BaCl2 (Ba++), cultured in growth medium and normalized to ASCs on tissue culture polystyrene (TCPS). (C) Growth factor retained within microbeads normalized to DNA content (n = 6 samples, mean±SE, *p

Techniques Used: Cell Culture

2) Product Images from "The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit. The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit"

Article Title: The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit. The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit

Journal: Journal of Thrombosis and Haemostasis

doi: 10.1111/jth.14128

Cross‐linking of fibrin by full fength rFXIIIA and fragments. Clots were formed with fibrinogen, thrombin, CaCl2, and either full length rFXIIIA (lane 2), fragment TB 1 (lacking barrel 2; lane 3), TCC (lacking barrels 1 and 2; lane 4), TBS (lacking catalytic core and barrels 1 and 2; lane 5), or a control without any form of FXIII (lane 6). After 30 min, samples were run on an SDS ‐ PAGE gel under reducing conditions (A). The mean density of the different bands was determined relative to the control for that peptide chain or cross‐linked structure (B). FXIIIA , full length FXIII A subunit; TB 1, full length FXIII A subunit lacking barrel 2; TBS , full length FXIII A subunit lacking barrels 1 and 2, and catalytic core; TCC , full length FXIII A subunit lacking barrels 1 and 2.
Figure Legend Snippet: Cross‐linking of fibrin by full fength rFXIIIA and fragments. Clots were formed with fibrinogen, thrombin, CaCl2, and either full length rFXIIIA (lane 2), fragment TB 1 (lacking barrel 2; lane 3), TCC (lacking barrels 1 and 2; lane 4), TBS (lacking catalytic core and barrels 1 and 2; lane 5), or a control without any form of FXIII (lane 6). After 30 min, samples were run on an SDS ‐ PAGE gel under reducing conditions (A). The mean density of the different bands was determined relative to the control for that peptide chain or cross‐linked structure (B). FXIIIA , full length FXIII A subunit; TB 1, full length FXIII A subunit lacking barrel 2; TBS , full length FXIII A subunit lacking barrels 1 and 2, and catalytic core; TCC , full length FXIII A subunit lacking barrels 1 and 2.

Techniques Used: SDS Page

Effect of recombinant Factor XIIIA and fragments on turbidity and clot lysis. Polymerisation of FXIIIA depleted fibrinogen was initiated by the addition of thrombin, CaCl2, and either full length rFXIIIA (black square), fragment TB 1 (lacking barrel 2; dark grey diamond), TCC (lacking barrels 1 and 2; mid‐grey triangle), TBS (lacking catalytic core and barrels 1 and 2; light grey circles), or a control (buffer; very light grey crosses). Generation of turbidity was measured every 12 s for 60 min and results shown are mean values of triplicate experiments (A). Only clots with full‐length FXIII ‐A had a significant decrease in final maximum absorbance compared to control ( n = 3, P
Figure Legend Snippet: Effect of recombinant Factor XIIIA and fragments on turbidity and clot lysis. Polymerisation of FXIIIA depleted fibrinogen was initiated by the addition of thrombin, CaCl2, and either full length rFXIIIA (black square), fragment TB 1 (lacking barrel 2; dark grey diamond), TCC (lacking barrels 1 and 2; mid‐grey triangle), TBS (lacking catalytic core and barrels 1 and 2; light grey circles), or a control (buffer; very light grey crosses). Generation of turbidity was measured every 12 s for 60 min and results shown are mean values of triplicate experiments (A). Only clots with full‐length FXIII ‐A had a significant decrease in final maximum absorbance compared to control ( n = 3, P

Techniques Used: Recombinant, Lysis

3) Product Images from "The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit. The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit"

Article Title: The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit. The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit

Journal: Journal of Thrombosis and Haemostasis

doi: 10.1111/jth.14128

Cross‐linking of fibrin by full fength rFXIIIA and fragments. Clots were formed with fibrinogen, thrombin, CaCl2, and either full length rFXIIIA (lane 2), fragment TB 1 (lacking barrel 2; lane 3), TCC (lacking barrels 1 and 2; lane 4), TBS (lacking catalytic core and barrels 1 and 2; lane 5), or a control without any form of FXIII (lane 6). After 30 min, samples were run on an SDS ‐ PAGE gel under reducing conditions (A). The mean density of the different bands was determined relative to the control for that peptide chain or cross‐linked structure (B). FXIIIA , full length FXIII A subunit; TB 1, full length FXIII A subunit lacking barrel 2; TBS , full length FXIII A subunit lacking barrels 1 and 2, and catalytic core; TCC , full length FXIII A subunit lacking barrels 1 and 2.
Figure Legend Snippet: Cross‐linking of fibrin by full fength rFXIIIA and fragments. Clots were formed with fibrinogen, thrombin, CaCl2, and either full length rFXIIIA (lane 2), fragment TB 1 (lacking barrel 2; lane 3), TCC (lacking barrels 1 and 2; lane 4), TBS (lacking catalytic core and barrels 1 and 2; lane 5), or a control without any form of FXIII (lane 6). After 30 min, samples were run on an SDS ‐ PAGE gel under reducing conditions (A). The mean density of the different bands was determined relative to the control for that peptide chain or cross‐linked structure (B). FXIIIA , full length FXIII A subunit; TB 1, full length FXIII A subunit lacking barrel 2; TBS , full length FXIII A subunit lacking barrels 1 and 2, and catalytic core; TCC , full length FXIII A subunit lacking barrels 1 and 2.

Techniques Used: SDS Page

Effect of recombinant Factor XIIIA and fragments on turbidity and clot lysis. Polymerisation of FXIIIA depleted fibrinogen was initiated by the addition of thrombin, CaCl2, and either full length rFXIIIA (black square), fragment TB 1 (lacking barrel 2; dark grey diamond), TCC (lacking barrels 1 and 2; mid‐grey triangle), TBS (lacking catalytic core and barrels 1 and 2; light grey circles), or a control (buffer; very light grey crosses). Generation of turbidity was measured every 12 s for 60 min and results shown are mean values of triplicate experiments (A). Only clots with full‐length FXIII ‐A had a significant decrease in final maximum absorbance compared to control ( n = 3, P
Figure Legend Snippet: Effect of recombinant Factor XIIIA and fragments on turbidity and clot lysis. Polymerisation of FXIIIA depleted fibrinogen was initiated by the addition of thrombin, CaCl2, and either full length rFXIIIA (black square), fragment TB 1 (lacking barrel 2; dark grey diamond), TCC (lacking barrels 1 and 2; mid‐grey triangle), TBS (lacking catalytic core and barrels 1 and 2; light grey circles), or a control (buffer; very light grey crosses). Generation of turbidity was measured every 12 s for 60 min and results shown are mean values of triplicate experiments (A). Only clots with full‐length FXIII ‐A had a significant decrease in final maximum absorbance compared to control ( n = 3, P

Techniques Used: Recombinant, Lysis

4) Product Images from "Adipose Stem Cell Microbeads as Production Sources for Chondrogenic Growth Factors"

Article Title: Adipose Stem Cell Microbeads as Production Sources for Chondrogenic Growth Factors

Journal: Journal of Stem Cells & Regenerative Medicine

doi:

Effect of Divalent Crosslinks in ASC Microbead on Growth Factor mRNA Levels and Production. (A) mRNA levels and (B) growth factor secretion/DNA content on day 7 from ASC microbeads crosslinked in CaCl2 Ca++) or BaCl2 (Ba++), cultured in growth medium and normalized to ASCs on tissue culture polystyrene (TCPS). (C) Growth factor retained within microbeads normalized to DNA content (n = 6 samples, mean±SE, *p
Figure Legend Snippet: Effect of Divalent Crosslinks in ASC Microbead on Growth Factor mRNA Levels and Production. (A) mRNA levels and (B) growth factor secretion/DNA content on day 7 from ASC microbeads crosslinked in CaCl2 Ca++) or BaCl2 (Ba++), cultured in growth medium and normalized to ASCs on tissue culture polystyrene (TCPS). (C) Growth factor retained within microbeads normalized to DNA content (n = 6 samples, mean±SE, *p

Techniques Used: Cell Culture

5) Product Images from "Mover Is a Homomeric Phospho-Protein Present on Synaptic Vesicles"

Article Title: Mover Is a Homomeric Phospho-Protein Present on Synaptic Vesicles

Journal: PLoS ONE

doi: 10.1371/journal.pone.0063474

Mover does not dissociate from synaptic vesicles in response to depolarization. (A) Assay of glutamate release from synaptosomes using the fluorescence-based NADPH assay [26] to verify that SVs in synaptosomes undergo calcium-dependent fusion and exocytosis to release glutamate. Depolarization with 50 mM KCl induces glutamate release in the presence of CaCl2, but not of EGTA. (B) Synaptosomal preparations were incubated for 10 min at 37°C in control conditions, in the presence of 1 mM EGTA to chelate calcium, in 1 µM okadaic acid to phosphorylate proteins, or in depolarizing conditions. Following treatment, each synaptosomal fraction was further fractionated to obtain a crude SV fraction. Equal volumes of the crude SV fractions were then subjected to Western blot analysis to test for Mover and synapsin protein levels associated with vesicles. Mover did not dissociate from vesicles in response to depolarization, whereas synapsin did. N = 2 experiments.
Figure Legend Snippet: Mover does not dissociate from synaptic vesicles in response to depolarization. (A) Assay of glutamate release from synaptosomes using the fluorescence-based NADPH assay [26] to verify that SVs in synaptosomes undergo calcium-dependent fusion and exocytosis to release glutamate. Depolarization with 50 mM KCl induces glutamate release in the presence of CaCl2, but not of EGTA. (B) Synaptosomal preparations were incubated for 10 min at 37°C in control conditions, in the presence of 1 mM EGTA to chelate calcium, in 1 µM okadaic acid to phosphorylate proteins, or in depolarizing conditions. Following treatment, each synaptosomal fraction was further fractionated to obtain a crude SV fraction. Equal volumes of the crude SV fractions were then subjected to Western blot analysis to test for Mover and synapsin protein levels associated with vesicles. Mover did not dissociate from vesicles in response to depolarization, whereas synapsin did. N = 2 experiments.

Techniques Used: Fluorescence, Incubation, Western Blot

6) Product Images from "Tonic NMDA receptor signalling shapes endosomal organisation in mammalian cells"

Article Title: Tonic NMDA receptor signalling shapes endosomal organisation in mammalian cells

Journal: Scientific Reports

doi: 10.1038/s41598-020-66071-0

Ca 2+ influx through NMDARs rapidly regulates EEs. ( A ) Representative image of primary human fibroblasts incubated for 10 min in PBS or PBS supplemented with 1.8 mM CaCl 2 . ( B ) Left, quantification of EEA1 immunostaining levels in cells incubated in PBS with or without added CaCl2. Right, quantification of the EEA1 area in cells incubated in PBS with or without added CaCl 2 . N = 50–250 EEs/image, 20 images/condition, 4 independent experiments. ( C ) Quantification of the EEA1 immunostaining levels in cells following incubation for 10 min in DMEM + 10% FBS in presence of indicated drugs. N = 50–250 EEs/image, 25 images/condition, 5 independent experiments. ( D ) Immunostaining for endogenous GRIN1 in fibroblasts. Live cells were incubated with fluorescent Transferrin (Tf) for 1 h at 37 °C, fixed and immunostained for GRIN1 and EEA1. Arrows denote GRIN1 signal colocalising with EEA1- and Tf-positive puncta. ( E ) Dissociated rat hippocampal neurons (21 days in vitro ) were treated with MK801 for 30 min at 37 °C. ( F ) Quantification of the EEA1 immunostaining levels in neurons treated with MK801. N = 20–100 EEs/image, 15 images/condition, 3 independent experiments. ***P
Figure Legend Snippet: Ca 2+ influx through NMDARs rapidly regulates EEs. ( A ) Representative image of primary human fibroblasts incubated for 10 min in PBS or PBS supplemented with 1.8 mM CaCl 2 . ( B ) Left, quantification of EEA1 immunostaining levels in cells incubated in PBS with or without added CaCl2. Right, quantification of the EEA1 area in cells incubated in PBS with or without added CaCl 2 . N = 50–250 EEs/image, 20 images/condition, 4 independent experiments. ( C ) Quantification of the EEA1 immunostaining levels in cells following incubation for 10 min in DMEM + 10% FBS in presence of indicated drugs. N = 50–250 EEs/image, 25 images/condition, 5 independent experiments. ( D ) Immunostaining for endogenous GRIN1 in fibroblasts. Live cells were incubated with fluorescent Transferrin (Tf) for 1 h at 37 °C, fixed and immunostained for GRIN1 and EEA1. Arrows denote GRIN1 signal colocalising with EEA1- and Tf-positive puncta. ( E ) Dissociated rat hippocampal neurons (21 days in vitro ) were treated with MK801 for 30 min at 37 °C. ( F ) Quantification of the EEA1 immunostaining levels in neurons treated with MK801. N = 20–100 EEs/image, 15 images/condition, 3 independent experiments. ***P

Techniques Used: Incubation, Immunostaining, In Vitro

Related Articles

Isolation:

Article Title: Adipose Stem Cell Microbeads as Production Sources for Chondrogenic Growth Factors
Article Snippet: .. RNA Isolation Alginate microbeads crosslinked in CaCl2 (Ca microbeads) were uncrosslinked in 82.5 mM sodium citrate (Sigma) whereas alginate microbeads crosslinked in BaCl2 (Ba microbeads) were uncrosslinked in 30 mM ethylenediaminetetraacetic acid (EDTA, Sigma) and 135 mM NaCl (Sigma). ..

Incubation:

Article Title: Mover Is a Homomeric Phospho-Protein Present on Synaptic Vesicles
Article Snippet: .. Subsequently, 1.3 mM CaCl2 or 0.5 mM EGTA was added, with glutamate dehydrogenase (Sigma type II, 34 U) and 1 mM NADP, and solutions were incubated for 4 min. A final concentration of 50 mM KCl was then added as indicated. ..

Article Title: The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit. The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit
Article Snippet: .. Briefly, microtiter plates were coated with 40 μg mL−1 human fibrinogen (Enzyme Research Laboratories) at 37 °C for 1 h. After blocking with 1% BSA, plates were incubated with 1 U mL−1 human thrombin (Calbiochem) and 5 mm CaCl2 to convert fibrinogen to fibrin, and then treated in triplicate with 3.5 nm recombinant FXIII‐A sample, 10 μg mL−1 α2 ‐antiplasmin (Calbiochem), 1 U mL−1 human thrombin, 0.1 mm DTT, and 1 mm CaCl2 . .. Incorporation of α2 ‐antiplasmin was stopped with 133 mm EDTA over a time course of 50 min. Crosslinking of the α2 ‐antiplasmin into the fibrin by recombinant FXIII was detected by the use of goat anti‐human α2 ‐antiplasmin antibody with a horseradish peroxidase conjugate (Enzyme Research Laboratories) and o ‐phenylenediamine (OPD) (Dako, Ely, UK).

Blocking Assay:

Article Title: The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit. The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit
Article Snippet: .. Briefly, microtiter plates were coated with 40 μg mL−1 human fibrinogen (Enzyme Research Laboratories) at 37 °C for 1 h. After blocking with 1% BSA, plates were incubated with 1 U mL−1 human thrombin (Calbiochem) and 5 mm CaCl2 to convert fibrinogen to fibrin, and then treated in triplicate with 3.5 nm recombinant FXIII‐A sample, 10 μg mL−1 α2 ‐antiplasmin (Calbiochem), 1 U mL−1 human thrombin, 0.1 mm DTT, and 1 mm CaCl2 . .. Incorporation of α2 ‐antiplasmin was stopped with 133 mm EDTA over a time course of 50 min. Crosslinking of the α2 ‐antiplasmin into the fibrin by recombinant FXIII was detected by the use of goat anti‐human α2 ‐antiplasmin antibody with a horseradish peroxidase conjugate (Enzyme Research Laboratories) and o ‐phenylenediamine (OPD) (Dako, Ely, UK).

Concentration Assay:

Article Title: Mover Is a Homomeric Phospho-Protein Present on Synaptic Vesicles
Article Snippet: .. Subsequently, 1.3 mM CaCl2 or 0.5 mM EGTA was added, with glutamate dehydrogenase (Sigma type II, 34 U) and 1 mM NADP, and solutions were incubated for 4 min. A final concentration of 50 mM KCl was then added as indicated. ..

Generated:

Article Title: Effect of IAPP on the proteome of cultured Rin-5F cells
Article Snippet: .. Proteins were stained using 0.5% Coomassie Brilliant Blue G250 (Sigma, UK), 40% ethanol and 10% acetic acid for 1 h, and destained in 20% ethanol and 10% acetic acid for 2 h. To digest the proteins and isolate the resulting tryptic peptides generated from OFFGEL™ fractionation, the larger of the acetone precipitated pellets obtained from each OFFGEL™ fraction were reconstituted in 150 μl of 1X digestion buffer containing 1 M ammonium bicarbonate, 1 M CaCl2 and 1.2 g urea (all from Sigma, UK). ..

other:

Article Title: Unraveling essential cellulosomal components of the (Pseudo)Bacteroides cellulosolvens reveals an extensive reservoir of novel catalytic enzymes
Article Snippet: Anaerobic fermentation Bacteroides cellulosolvens was grown on 315 medium (DSMZ) containing (per liter distilled water): 0.68 g NH4 Cl, 0.30 g K2 HPO4 , 0.18 g KH2 PO4 , 0.15 g (NH4 )2 SO4 , 0.12 g MgSO4 × 7H2 O, 0.06 g CaCl2 × 2H2 O, 0.02 g FeSO4 × 7H2 O, 10 ml trace element solution (see below), 10 ml BME vitamin solution (Sigma), 5 g cellobiose or 5 g cellulose, 1 mg resazurin, 2 g NaHCO3 , 0.25 g cysteine-HCl × H2 O, and 0.25 g Na2 S × 9H2 O.

Article Title: Tonic NMDA receptor signalling shapes endosomal organisation in mammalian cells
Article Snippet: CdCl2 , CaCl2 and FITC-cholera toxin B subunit were from Sigma-Aldrich (UK).

Fractionation:

Article Title: Effect of IAPP on the proteome of cultured Rin-5F cells
Article Snippet: .. Proteins were stained using 0.5% Coomassie Brilliant Blue G250 (Sigma, UK), 40% ethanol and 10% acetic acid for 1 h, and destained in 20% ethanol and 10% acetic acid for 2 h. To digest the proteins and isolate the resulting tryptic peptides generated from OFFGEL™ fractionation, the larger of the acetone precipitated pellets obtained from each OFFGEL™ fraction were reconstituted in 150 μl of 1X digestion buffer containing 1 M ammonium bicarbonate, 1 M CaCl2 and 1.2 g urea (all from Sigma, UK). ..

Staining:

Article Title: Effect of IAPP on the proteome of cultured Rin-5F cells
Article Snippet: .. Proteins were stained using 0.5% Coomassie Brilliant Blue G250 (Sigma, UK), 40% ethanol and 10% acetic acid for 1 h, and destained in 20% ethanol and 10% acetic acid for 2 h. To digest the proteins and isolate the resulting tryptic peptides generated from OFFGEL™ fractionation, the larger of the acetone precipitated pellets obtained from each OFFGEL™ fraction were reconstituted in 150 μl of 1X digestion buffer containing 1 M ammonium bicarbonate, 1 M CaCl2 and 1.2 g urea (all from Sigma, UK). ..

Recombinant:

Article Title: The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit. The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit
Article Snippet: .. Briefly, microtiter plates were coated with 40 μg mL−1 human fibrinogen (Enzyme Research Laboratories) at 37 °C for 1 h. After blocking with 1% BSA, plates were incubated with 1 U mL−1 human thrombin (Calbiochem) and 5 mm CaCl2 to convert fibrinogen to fibrin, and then treated in triplicate with 3.5 nm recombinant FXIII‐A sample, 10 μg mL−1 α2 ‐antiplasmin (Calbiochem), 1 U mL−1 human thrombin, 0.1 mm DTT, and 1 mm CaCl2 . .. Incorporation of α2 ‐antiplasmin was stopped with 133 mm EDTA over a time course of 50 min. Crosslinking of the α2 ‐antiplasmin into the fibrin by recombinant FXIII was detected by the use of goat anti‐human α2 ‐antiplasmin antibody with a horseradish peroxidase conjugate (Enzyme Research Laboratories) and o ‐phenylenediamine (OPD) (Dako, Ely, UK).

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  • 88
    Millipore tppe digestion buffer
    <t>TPPE</t> cleaves the reduced pro-BTH6 in the thionin domain. LC-ESI-MS analysis of the digestion of <t>carboxymethylated</t> myc-proBTH6-strep with recombinant TPPE. The acidic domain is cleaved several times with additional sites compared with the oxidized proprotein.
    Tppe Digestion Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tppe digestion buffer/product/Millipore
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    90
    Millipore cytokine homogenate buffer
    Leptin and <t>cytokine</t> levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.
    Cytokine Homogenate Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokine homogenate buffer/product/Millipore
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    Price from $9.99 to $1999.99
    cytokine homogenate buffer - by Bioz Stars, 2020-07
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    88
    Millipore glucose free artificial csf
    Native human Aß stimulated glucose uptake in neurons. (A) Summary of the cases used in this study. <t>CSF</t> samples were from the Department of Neurological and Psychiatric Sciences, University of Florence. Clinical diagnosis of AD was defined according to the Diagnostic and Statistical Manual of Mental Disorders criteria (DSM-IV). Clinical diagnosis of MCI was defined according to Petersen's validated criteria (Petersen et al., 2001 ). CSF Aß 1−42 was quantitated by INNOTEST ß-amyloid (1–42) from Innogenetics. (B) Contribution of CSF Aß monomers to depolarization-induced neuronal glucose uptake both in the absence (IgG2b isotype control) and in the presence (4G8) of a neutralizing anti-Aß antibody. Neurons were pre-exposed to the γ-secretase inhibitor IX and underwent a 15 min depolarization pulse with 400 mM <t>KCl</t> (γ-Sec Inh + KCl). Mean values of glucose uptake (mg/dl) after the pulse were the following: 47.75 ± 4.77 vs. 33.87 ± 4.54 * ( * different at p
    Glucose Free Artificial Csf, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glucose free artificial csf/product/Millipore
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glucose free artificial csf - by Bioz Stars, 2020-07
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    94
    Millipore cacl2
    Cross‐linking of fibrin by full fength rFXIIIA and fragments. Clots were formed with fibrinogen, thrombin, <t>CaCl2,</t> and either full length rFXIIIA (lane 2), fragment TB 1 (lacking barrel 2; lane 3), TCC (lacking barrels 1 and 2; lane 4), TBS (lacking catalytic core and barrels 1 and 2; lane 5), or a control without any form of FXIII (lane 6). After 30 min, samples were run on an SDS ‐ PAGE gel under reducing conditions (A). The mean density of the different bands was determined relative to the control for that peptide chain or cross‐linked structure (B). FXIIIA , full length FXIII A subunit; TB 1, full length FXIII A subunit lacking barrel 2; TBS , full length FXIII A subunit lacking barrels 1 and 2, and catalytic core; TCC , full length FXIII A subunit lacking barrels 1 and 2.
    Cacl2, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacl2/product/Millipore
    Average 94 stars, based on 447 article reviews
    Price from $9.99 to $1999.99
    cacl2 - by Bioz Stars, 2020-07
    94/100 stars
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    Image Search Results


    TPPE cleaves the reduced pro-BTH6 in the thionin domain. LC-ESI-MS analysis of the digestion of carboxymethylated myc-proBTH6-strep with recombinant TPPE. The acidic domain is cleaved several times with additional sites compared with the oxidized proprotein.

    Journal: The Journal of Biological Chemistry

    Article Title: Isolation and Characterization of a Thionin Proprotein-processing Enzyme from Barley *

    doi: 10.1074/jbc.M115.647859

    Figure Lengend Snippet: TPPE cleaves the reduced pro-BTH6 in the thionin domain. LC-ESI-MS analysis of the digestion of carboxymethylated myc-proBTH6-strep with recombinant TPPE. The acidic domain is cleaved several times with additional sites compared with the oxidized proprotein.

    Article Snippet: The reduced and cysteine-carboxymethylated protein samples were dialyzed against TPPE digestion buffer (25 m m MES, 100 m m NaCl, 10 m m CaCl2 , pH 6.5) using Amicon Ultra 10K ultrafiltration centrifugal filters (Millipore) according to the recommendations of the manufacturer.

    Techniques: Mass Spectrometry, Recombinant

    Leptin and cytokine levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.

    Journal: BioMed Research International

    Article Title: Advanced Glycation End Products Induce Obesity and Hepatosteatosis in CD-1 Wild-Type Mice

    doi: 10.1155/2016/7867852

    Figure Lengend Snippet: Leptin and cytokine levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.

    Article Snippet: The remaining liver was washed with cold HBSS and suspended in cytokine homogenate buffer (150 mM NaCl, 15 mM Tris, 1 mM CaCl2 ·2H2 O, and 1 mM MgCl2 ·6H2 O, adjusted to pH 7.4) plus 100x protease inhibitor cocktail 1 (Calbiochem, La Jolla, California) to a total volume of 10 mL and homogenized on ice using a Polytron® Homogenizer (Kinematica Inc., Bohemia, NY).

    Techniques:

    Native human Aß stimulated glucose uptake in neurons. (A) Summary of the cases used in this study. CSF samples were from the Department of Neurological and Psychiatric Sciences, University of Florence. Clinical diagnosis of AD was defined according to the Diagnostic and Statistical Manual of Mental Disorders criteria (DSM-IV). Clinical diagnosis of MCI was defined according to Petersen's validated criteria (Petersen et al., 2001 ). CSF Aß 1−42 was quantitated by INNOTEST ß-amyloid (1–42) from Innogenetics. (B) Contribution of CSF Aß monomers to depolarization-induced neuronal glucose uptake both in the absence (IgG2b isotype control) and in the presence (4G8) of a neutralizing anti-Aß antibody. Neurons were pre-exposed to the γ-secretase inhibitor IX and underwent a 15 min depolarization pulse with 400 mM KCl (γ-Sec Inh + KCl). Mean values of glucose uptake (mg/dl) after the pulse were the following: 47.75 ± 4.77 vs. 33.87 ± 4.54 * ( * different at p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Monomeric ß-amyloid interacts with type-1 insulin-like growth factor receptors to provide energy supply to neurons

    doi: 10.3389/fncel.2015.00297

    Figure Lengend Snippet: Native human Aß stimulated glucose uptake in neurons. (A) Summary of the cases used in this study. CSF samples were from the Department of Neurological and Psychiatric Sciences, University of Florence. Clinical diagnosis of AD was defined according to the Diagnostic and Statistical Manual of Mental Disorders criteria (DSM-IV). Clinical diagnosis of MCI was defined according to Petersen's validated criteria (Petersen et al., 2001 ). CSF Aß 1−42 was quantitated by INNOTEST ß-amyloid (1–42) from Innogenetics. (B) Contribution of CSF Aß monomers to depolarization-induced neuronal glucose uptake both in the absence (IgG2b isotype control) and in the presence (4G8) of a neutralizing anti-Aß antibody. Neurons were pre-exposed to the γ-secretase inhibitor IX and underwent a 15 min depolarization pulse with 400 mM KCl (γ-Sec Inh + KCl). Mean values of glucose uptake (mg/dl) after the pulse were the following: 47.75 ± 4.77 vs. 33.87 ± 4.54 * ( * different at p

    Article Snippet: For the experiment, cultures were rinsed in glucose-free artificial CSF (124 mM NaCl, 2.5 mM KCl, 2 mM MgSO4 , 1.25 mM KH2 PO4 , 26 mM NaHCO3, 2.5 mM CaCl2 ) and maintained for 45 min under glucose deprivation. γ-Sec-inhibitor IX (Calbiochem, 100 nM) was added 2 h before glucose deprivation and maintained troughout the experiment.

    Techniques: Diagnostic Assay, Size-exclusion Chromatography

    Cross‐linking of fibrin by full fength rFXIIIA and fragments. Clots were formed with fibrinogen, thrombin, CaCl2, and either full length rFXIIIA (lane 2), fragment TB 1 (lacking barrel 2; lane 3), TCC (lacking barrels 1 and 2; lane 4), TBS (lacking catalytic core and barrels 1 and 2; lane 5), or a control without any form of FXIII (lane 6). After 30 min, samples were run on an SDS ‐ PAGE gel under reducing conditions (A). The mean density of the different bands was determined relative to the control for that peptide chain or cross‐linked structure (B). FXIIIA , full length FXIII A subunit; TB 1, full length FXIII A subunit lacking barrel 2; TBS , full length FXIII A subunit lacking barrels 1 and 2, and catalytic core; TCC , full length FXIII A subunit lacking barrels 1 and 2.

    Journal: Journal of Thrombosis and Haemostasis

    Article Title: The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit. The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit

    doi: 10.1111/jth.14128

    Figure Lengend Snippet: Cross‐linking of fibrin by full fength rFXIIIA and fragments. Clots were formed with fibrinogen, thrombin, CaCl2, and either full length rFXIIIA (lane 2), fragment TB 1 (lacking barrel 2; lane 3), TCC (lacking barrels 1 and 2; lane 4), TBS (lacking catalytic core and barrels 1 and 2; lane 5), or a control without any form of FXIII (lane 6). After 30 min, samples were run on an SDS ‐ PAGE gel under reducing conditions (A). The mean density of the different bands was determined relative to the control for that peptide chain or cross‐linked structure (B). FXIIIA , full length FXIII A subunit; TB 1, full length FXIII A subunit lacking barrel 2; TBS , full length FXIII A subunit lacking barrels 1 and 2, and catalytic core; TCC , full length FXIII A subunit lacking barrels 1 and 2.

    Article Snippet: Briefly, microtiter plates were coated with 40 μg mL−1 human fibrinogen (Enzyme Research Laboratories) at 37 °C for 1 h. After blocking with 1% BSA, plates were incubated with 1 U mL−1 human thrombin (Calbiochem) and 5 mm CaCl2 to convert fibrinogen to fibrin, and then treated in triplicate with 3.5 nm recombinant FXIII‐A sample, 10 μg mL−1 α2 ‐antiplasmin (Calbiochem), 1 U mL−1 human thrombin, 0.1 mm DTT, and 1 mm CaCl2 .

    Techniques: SDS Page

    Effect of recombinant Factor XIIIA and fragments on turbidity and clot lysis. Polymerisation of FXIIIA depleted fibrinogen was initiated by the addition of thrombin, CaCl2, and either full length rFXIIIA (black square), fragment TB 1 (lacking barrel 2; dark grey diamond), TCC (lacking barrels 1 and 2; mid‐grey triangle), TBS (lacking catalytic core and barrels 1 and 2; light grey circles), or a control (buffer; very light grey crosses). Generation of turbidity was measured every 12 s for 60 min and results shown are mean values of triplicate experiments (A). Only clots with full‐length FXIII ‐A had a significant decrease in final maximum absorbance compared to control ( n = 3, P

    Journal: Journal of Thrombosis and Haemostasis

    Article Title: The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit. The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit

    doi: 10.1111/jth.14128

    Figure Lengend Snippet: Effect of recombinant Factor XIIIA and fragments on turbidity and clot lysis. Polymerisation of FXIIIA depleted fibrinogen was initiated by the addition of thrombin, CaCl2, and either full length rFXIIIA (black square), fragment TB 1 (lacking barrel 2; dark grey diamond), TCC (lacking barrels 1 and 2; mid‐grey triangle), TBS (lacking catalytic core and barrels 1 and 2; light grey circles), or a control (buffer; very light grey crosses). Generation of turbidity was measured every 12 s for 60 min and results shown are mean values of triplicate experiments (A). Only clots with full‐length FXIII ‐A had a significant decrease in final maximum absorbance compared to control ( n = 3, P

    Article Snippet: Briefly, microtiter plates were coated with 40 μg mL−1 human fibrinogen (Enzyme Research Laboratories) at 37 °C for 1 h. After blocking with 1% BSA, plates were incubated with 1 U mL−1 human thrombin (Calbiochem) and 5 mm CaCl2 to convert fibrinogen to fibrin, and then treated in triplicate with 3.5 nm recombinant FXIII‐A sample, 10 μg mL−1 α2 ‐antiplasmin (Calbiochem), 1 U mL−1 human thrombin, 0.1 mm DTT, and 1 mm CaCl2 .

    Techniques: Recombinant, Lysis