cacl2 solution  (Millipore)


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    Structured Review

    Millipore cacl2 solution
    Cacl2 Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacl2 solution/product/Millipore
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    cacl2 solution - by Bioz Stars, 2020-09
    99/100 stars

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    Incubation:

    Article Title: A Conventional Beagle Dog Model for Acute and Chronic Infection with Helicobacter pylori
    Article Snippet: .. Antral biopsies were incubated for up to 24 h in 1 ml of a 10% urea solution in distilled water to which 2 drops of a 1% phenol red solution (Sigma) in sodium phosphate buffer (pH 6.5) had been added. ..

    Article Title: The Role of Candida albicans NOT5 in Virulence Depends upon Diverse Host Factors In Vivo
    Article Snippet: .. The cell pellet was harvested, washed, resuspended in BugBuster solution (EMD Biosciences, Inc.), and incubated briefly at room temperature. ..

    Centrifugation:

    Article Title: The Activity and Specificity of the Outer Membrane Protein Chaperone SurA Are Modulated by a Proline Isomerase Domain
    Article Snippet: .. As an alternative method, cells were grown to an optical density at 600 nm (OD600 ) of 1, harvested by centrifugation, washed in 20 mM potassium phosphate (pH 7.2) and 150 mM NaCl, resuspended in BugBuster solution (Novagen) containing lysozyme (5 µg/ml), DNase I (50 µg/ml), RNase I (50 µg/ml), and allowed to lyse gently by rocking for 15 min at room temperature. .. SDS-PAGE buffer was added to the samples, which were then boiled for 10 min prior to Western blot analysis as described above.

    Cell Culture:

    Article Title: Antibody humanization by molecular dynamics simulations—in‐silico guided selection of critical backmutations) Antibody humanization by molecular dynamics simulations—in‐silico guided selection of critical backmutations
    Article Snippet: .. To study the effect of BM in heavy chain FR of the superhumanized Ab2/3H6 variants, transient expression was performed in HEK293‐6E cells (NRC biotechnology Research Institute) (Durocher et al. , ) cultured in F17 medium (Invitrogen, Life Technologies, Cat. No. A13835‐01) supplemented with 4 mM L‐glutamine (Biochrom, Cat. No. K0302), 0.1% Kolliphor P188 (Sigma‐Aldrich, Cat. No. K4894), 15 mg/l phenol red (Sigma‐Aldrich, Cat. No. P0290) and 25 µg/ml G418 (Biochrom, Cat. No. A2912). .. Transfection of pCEP4 vector (Invitrogen, Cat. No. V044‐50) was mediated by polyethylenimine (PEI) transfection using linear 25‐kDa PEI (Polysciences, Cat. No. 23966).

    Expressing:

    Article Title: Antibody humanization by molecular dynamics simulations—in‐silico guided selection of critical backmutations) Antibody humanization by molecular dynamics simulations—in‐silico guided selection of critical backmutations
    Article Snippet: .. To study the effect of BM in heavy chain FR of the superhumanized Ab2/3H6 variants, transient expression was performed in HEK293‐6E cells (NRC biotechnology Research Institute) (Durocher et al. , ) cultured in F17 medium (Invitrogen, Life Technologies, Cat. No. A13835‐01) supplemented with 4 mM L‐glutamine (Biochrom, Cat. No. K0302), 0.1% Kolliphor P188 (Sigma‐Aldrich, Cat. No. K4894), 15 mg/l phenol red (Sigma‐Aldrich, Cat. No. P0290) and 25 µg/ml G418 (Biochrom, Cat. No. A2912). .. Transfection of pCEP4 vector (Invitrogen, Cat. No. V044‐50) was mediated by polyethylenimine (PEI) transfection using linear 25‐kDa PEI (Polysciences, Cat. No. 23966).

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    Millipore calpain activation solution
    Schematic representation of the proposed hypothetical cascade for DAergic vulnerability in PD. Given that environmental toxins, such as paraquat, interfere with calcium homeostasis via mitochondrial damage, the resulting increase in <t>calpain</t> activity may
    Calpain Activation Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calpain activation solution/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calpain activation solution - by Bioz Stars, 2020-09
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    84
    Millipore calcium ion solution for ise
    Schematic representation of the proposed hypothetical cascade for DAergic vulnerability in PD. Given that environmental toxins, such as paraquat, interfere with calcium homeostasis via mitochondrial damage, the resulting increase in <t>calpain</t> activity may
    Calcium Ion Solution For Ise, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calcium ion solution for ise/product/Millipore
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    92
    Millipore cacl2 solution
    Schematic representation of the proposed hypothetical cascade for DAergic vulnerability in PD. Given that environmental toxins, such as paraquat, interfere with calcium homeostasis via mitochondrial damage, the resulting increase in <t>calpain</t> activity may
    Cacl2 Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacl2 solution/product/Millipore
    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    cacl2 solution - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Schematic representation of the proposed hypothetical cascade for DAergic vulnerability in PD. Given that environmental toxins, such as paraquat, interfere with calcium homeostasis via mitochondrial damage, the resulting increase in calpain activity may

    Journal: Acta neuropathologica

    Article Title: Environmental neurotoxic challenge of conditional alpha-synuclein transgenic mice predicts a dopaminergic olfactory-striatal interplay in early PD

    doi: 10.1007/s00401-014-1255-5

    Figure Lengend Snippet: Schematic representation of the proposed hypothetical cascade for DAergic vulnerability in PD. Given that environmental toxins, such as paraquat, interfere with calcium homeostasis via mitochondrial damage, the resulting increase in calpain activity may

    Article Snippet: For detection of calpain-resistant α-syn, free-floating sections were incubated in calpain activation solution (20 mM tris/HCl pH 7.8, 3.6 mM CaCl2 , 100 mM KCl2 ), containing 150 μg/ml calpain I (208712, Calbiochem) for 16 h at 37 °C.

    Techniques: Activity Assay

    Effect of paraquat on calpain activation and accumulation of CT α-syn. a , b OB protein extracts from non-transgenic (WT), A30P and paraquat-treated A30P+P and dox-treated control (A30P+D+P, A30P+D) mice were loaded on a SDS-PAGE or c native PAGE.

    Journal: Acta neuropathologica

    Article Title: Environmental neurotoxic challenge of conditional alpha-synuclein transgenic mice predicts a dopaminergic olfactory-striatal interplay in early PD

    doi: 10.1007/s00401-014-1255-5

    Figure Lengend Snippet: Effect of paraquat on calpain activation and accumulation of CT α-syn. a , b OB protein extracts from non-transgenic (WT), A30P and paraquat-treated A30P+P and dox-treated control (A30P+D+P, A30P+D) mice were loaded on a SDS-PAGE or c native PAGE.

    Article Snippet: For detection of calpain-resistant α-syn, free-floating sections were incubated in calpain activation solution (20 mM tris/HCl pH 7.8, 3.6 mM CaCl2 , 100 mM KCl2 ), containing 150 μg/ml calpain I (208712, Calbiochem) for 16 h at 37 °C.

    Techniques: Activation Assay, Transgenic Assay, Mouse Assay, SDS Page, Clear Native PAGE

    A30P+P mice show elevated levels of calpain-resistant α-syn in the GL. a , c Representative sections of GL from A30P and A30P+P mice with and without treatment of calpain-1 and, b , d quantitative results. a After calpain1 digestion, brain sections

    Journal: Acta neuropathologica

    Article Title: Environmental neurotoxic challenge of conditional alpha-synuclein transgenic mice predicts a dopaminergic olfactory-striatal interplay in early PD

    doi: 10.1007/s00401-014-1255-5

    Figure Lengend Snippet: A30P+P mice show elevated levels of calpain-resistant α-syn in the GL. a , c Representative sections of GL from A30P and A30P+P mice with and without treatment of calpain-1 and, b , d quantitative results. a After calpain1 digestion, brain sections

    Article Snippet: For detection of calpain-resistant α-syn, free-floating sections were incubated in calpain activation solution (20 mM tris/HCl pH 7.8, 3.6 mM CaCl2 , 100 mM KCl2 ), containing 150 μg/ml calpain I (208712, Calbiochem) for 16 h at 37 °C.

    Techniques: Mouse Assay