cacl2 solution  (Millipore)


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    Structured Review

    Millipore cacl2 solution
    Cacl2 Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacl2 solution/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cacl2 solution - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Uncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen
    Article Snippet: The codon-optimized sequence the YFV structural genes was synthesized and cloned in pcDNA3.1(+) vector (Life Technologies, Carlsbad, CA, USA). .. Plasmid DNA was mixed with H2 O and 1 M CaCl2 solution (Sigma-Aldrich, St. Louis, MO, USA) and then an equal volume of 2x HEPES-buffered saline (pH 7.1, 140 mM NaCl (Sigma-Aldrich, St. Louis, MO, USA), 1.5 mM Na2 HPO4 (Sigma-Aldrich, St. Louis, MO, USA) and 50 mM HEPES (Sigma-Aldrich, St. Louis, MO, USA) was added to the mixture drop-wise with simultaneous vortexing.

    Cell Isolation:

    Article Title: Mesenchymal stromal cells lower platelet activation and assist in platelet formation in vitro
    Article Snippet: Paragraph title: Cell isolation from human UC tissue, cord blood, peripheral blood, and BM. ... To obtain a single cell suspension for subsequent cell sorting or flow cytometry analysis, artery segments were sequentially digested for 1.5 hours with PBS containing collagenase type 1 (562 U/ml; Invitrogen) and hyaluronidase (1 mg/ml; Thermo Fisher Scientific) and were supplemented with 1 M CaCl2 solution (MilliporeSigma) ( ).

    Amplification:

    Article Title: Uncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen
    Article Snippet: The resulting GFP-puromycin fusion construct was amplified by PCR using forward 5′-GCCCTACGCGTATGGTGAGCAAGGGC-3′ and reverse 5′-GCCCTACGCGTGGCACCGGGCTTGC-3′ primers and ligated into the MluI site of pWNVIIRep. .. Plasmid DNA was mixed with H2 O and 1 M CaCl2 solution (Sigma-Aldrich, St. Louis, MO, USA) and then an equal volume of 2x HEPES-buffered saline (pH 7.1, 140 mM NaCl (Sigma-Aldrich, St. Louis, MO, USA), 1.5 mM Na2 HPO4 (Sigma-Aldrich, St. Louis, MO, USA) and 50 mM HEPES (Sigma-Aldrich, St. Louis, MO, USA) was added to the mixture drop-wise with simultaneous vortexing.

    Synthesized:

    Article Title: Uncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen
    Article Snippet: The codon-optimized sequence the YFV structural genes was synthesized and cloned in pcDNA3.1(+) vector (Life Technologies, Carlsbad, CA, USA). .. Plasmid DNA was mixed with H2 O and 1 M CaCl2 solution (Sigma-Aldrich, St. Louis, MO, USA) and then an equal volume of 2x HEPES-buffered saline (pH 7.1, 140 mM NaCl (Sigma-Aldrich, St. Louis, MO, USA), 1.5 mM Na2 HPO4 (Sigma-Aldrich, St. Louis, MO, USA) and 50 mM HEPES (Sigma-Aldrich, St. Louis, MO, USA) was added to the mixture drop-wise with simultaneous vortexing.

    Article Title: Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells
    Article Snippet: LN from murine, CaCl2 solution, NaOH beads, acetone, absolute ethanol, and glutaraldehyde were obtained from Sigma-Aldrich (St Louis, MO, USA). .. Fluorescein isothiocyanate-conjugated LN (FITC-LN) was synthesized by Solarbio (Beijing, People’s Republic of China).

    BIA-KA:

    Article Title: Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells
    Article Snippet: Materials Dulbecco’s phosphate-buffered saline (DPBS, without calcium and magnesium), phosphate-buffered saline (PBS), accutase cell dissociation reagent, micro BCA protein assay kit (mBCA), serum albumin and live/dead viability/cytotoxicity kit were purchased from Thermo Fisher Scientific (Waltham, MA, USA). .. LN from murine, CaCl2 solution, NaOH beads, acetone, absolute ethanol, and glutaraldehyde were obtained from Sigma-Aldrich (St Louis, MO, USA).

    Cytometry:

    Article Title: Mesenchymal stromal cells lower platelet activation and assist in platelet formation in vitro
    Article Snippet: .. To obtain a single cell suspension for subsequent cell sorting or flow cytometry analysis, artery segments were sequentially digested for 1.5 hours with PBS containing collagenase type 1 (562 U/ml; Invitrogen) and hyaluronidase (1 mg/ml; Thermo Fisher Scientific) and were supplemented with 1 M CaCl2 solution (MilliporeSigma) ( ). .. Prior to cell culture, plates were coated with fibronectin (20 μg/ml; Stemcell Technologies) for 1 hour.

    Construct:

    Article Title: Uncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen
    Article Snippet: The resulting GFP-puromycin fusion construct was amplified by PCR using forward 5′-GCCCTACGCGTATGGTGAGCAAGGGC-3′ and reverse 5′-GCCCTACGCGTGGCACCGGGCTTGC-3′ primers and ligated into the MluI site of pWNVIIRep. .. Plasmid DNA was mixed with H2 O and 1 M CaCl2 solution (Sigma-Aldrich, St. Louis, MO, USA) and then an equal volume of 2x HEPES-buffered saline (pH 7.1, 140 mM NaCl (Sigma-Aldrich, St. Louis, MO, USA), 1.5 mM Na2 HPO4 (Sigma-Aldrich, St. Louis, MO, USA) and 50 mM HEPES (Sigma-Aldrich, St. Louis, MO, USA) was added to the mixture drop-wise with simultaneous vortexing.

    Incubation:

    Article Title: Uncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen
    Article Snippet: Plasmid DNA was mixed with H2 O and 1 M CaCl2 solution (Sigma-Aldrich, St. Louis, MO, USA) and then an equal volume of 2x HEPES-buffered saline (pH 7.1, 140 mM NaCl (Sigma-Aldrich, St. Louis, MO, USA), 1.5 mM Na2 HPO4 (Sigma-Aldrich, St. Louis, MO, USA) and 50 mM HEPES (Sigma-Aldrich, St. Louis, MO, USA) was added to the mixture drop-wise with simultaneous vortexing. .. The final concentration of CaCl2 in the transfection mix was 0.25 M. The resulting transfection mixture was incubated at room temperature for 20 min and then added to the cells.

    Article Title: Chitosan Enriched Three-Dimensional Matrix Reduces Inflammatory and Catabolic Mediators Production by Human Chondrocytes
    Article Snippet: This mixture was slowly passed dropwise through a syringe into a 102 mM CaCl2 solution (Sigma). .. After instantaneous gelation the beads were incubated for 10 min in the CaCl2 solution and washed with saline solution.

    Article Title: Localization of a bacterial protein in starch granules of transgenic maize kernels
    Article Snippet: .. To remove exogenous proteins from purified starch or ground maize kernel, samples were incubated with 5 mM CaCl2 solution containing 100 μg/ml thermolysin (Sigma) at 64°C for ≈16 h ( ). ..

    Article Title: The role of TGF‐β1/Smad2/3 pathway in platelet‐rich plasma in retarding intervertebral disc degeneration
    Article Snippet: .. The PRP was mixed well and incubated with 110 μl of 10% CaCl2 solution containing 100 U of bovine thrombin (Sigma‐Aldrich) at room temperature for 60 min. and then centrifuged at 500 × g for 10 min. ..

    Article Title: Effect of activated autologous platelet-rich plasma on proliferation and osteogenic differentiation of human adipose-derived stem cells in vitro
    Article Snippet: The PRP was activated with 10% CaCl2 solution (Sigma, St. Louis, MO, USA), 1 part CaCl2 stock solution was added to 9 parts PRP to mixing together. .. Each PRP sample was incubated for 1 hr at 37°C [ ].

    Expressing:

    Article Title: Dynamic Hydrostatic Pressure Regulates Nucleus Pulposus Phenotypic Expression and Metabolism in a Cell Density-Dependent Manner
    Article Snippet: Nucleus pulposus cells were resuspended in 1.2% w/v alginate solution (Sigma Aldrich, St. Louis, MO) at a density of either 1 M/mL or 4 M/mL and dispensed in 102 mM CaCl2 solution (Sigma Aldrich) via a single-needle infusion pump (Cole-Parmer, Vernon Hills, IL) at a constant flow rate of 6 mL/h through a 21G needle and syringe to yield ∼30 beads/mL of alginate solution. .. For cytotoxicity, gene expression, GAG, and collagen measurements, we utilized three beads per well and n = 3 wells per group were evaluated.

    Bradford Assay:

    Article Title: Localization of a bacterial protein in starch granules of transgenic maize kernels
    Article Snippet: Aqueous extractable protein was determined in all samples (drilled kernels, crushed endosperms, and starch) by using the Bradford assay ( ). .. To remove exogenous proteins from purified starch or ground maize kernel, samples were incubated with 5 mM CaCl2 solution containing 100 μg/ml thermolysin (Sigma) at 64°C for ≈16 h ( ).

    Western Blot:

    Article Title: Localization of a bacterial protein in starch granules of transgenic maize kernels
    Article Snippet: To remove exogenous proteins from purified starch or ground maize kernel, samples were incubated with 5 mM CaCl2 solution containing 100 μg/ml thermolysin (Sigma) at 64°C for ≈16 h ( ). .. Samples were subsequently washed five times in distilled water to remove thermolysin and were then boiled in SDS sample buffer ( ) (1 ml per 50 mg of starch) for 7 min at 95°C for Western analysis ( ).

    Derivative Assay:

    Article Title: Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells
    Article Snippet: LN from murine, CaCl2 solution, NaOH beads, acetone, absolute ethanol, and glutaraldehyde were obtained from Sigma-Aldrich (St Louis, MO, USA). .. NSC derived from brain tissues of Sprague Dawley (SD) rat embryos and NSC growth medium were obtained from Cyagen Biosciences (Soochow, People’s Republic of China).

    Flow Cytometry:

    Article Title: Mesenchymal stromal cells lower platelet activation and assist in platelet formation in vitro
    Article Snippet: .. To obtain a single cell suspension for subsequent cell sorting or flow cytometry analysis, artery segments were sequentially digested for 1.5 hours with PBS containing collagenase type 1 (562 U/ml; Invitrogen) and hyaluronidase (1 mg/ml; Thermo Fisher Scientific) and were supplemented with 1 M CaCl2 solution (MilliporeSigma) ( ). .. Prior to cell culture, plates were coated with fibronectin (20 μg/ml; Stemcell Technologies) for 1 hour.

    Article Title: Dynamic Hydrostatic Pressure Regulates Nucleus Pulposus Phenotypic Expression and Metabolism in a Cell Density-Dependent Manner
    Article Snippet: .. Nucleus pulposus cells were resuspended in 1.2% w/v alginate solution (Sigma Aldrich, St. Louis, MO) at a density of either 1 M/mL or 4 M/mL and dispensed in 102 mM CaCl2 solution (Sigma Aldrich) via a single-needle infusion pump (Cole-Parmer, Vernon Hills, IL) at a constant flow rate of 6 mL/h through a 21G needle and syringe to yield ∼30 beads/mL of alginate solution. .. Beads were cultured in complete media for 24 h and were then moved to multiwell tissue culture plates for loading studies.

    Article Title: Fabrication of 3-dimensional multicellular microvascular structures
    Article Snippet: In brief, 1.5 weight-percent alginate (Sigma-Aldrich) was in-line mixed 2 weight-percent fibrinogen (Sigma-Aldrich) at flow rates of 2 and 1 ml/h, respectively. .. The solution jet was collected in a grounded, rotating bath (30–45 rotations per minute) containing 50 mM CaCl2 solution with 10 U/ml thrombin (Sigma-Aldrich) for 35 minutes.

    Protease Inhibitor:

    Article Title: Localization of a bacterial protein in starch granules of transgenic maize kernels
    Article Snippet: Total proteins from kernel materials were extracted by using the following buffer: 25 mM sodium phosphate (pH 6.6)/100 mM NaCl/0.1% Triton X-100 (vol/vol)/1 mM EDTA/10 μg/ml of leupeptin (wt/vol)/0.25 mM serine protease inhibitor Perfabloc SC (Fluka), at room temperature. .. To remove exogenous proteins from purified starch or ground maize kernel, samples were incubated with 5 mM CaCl2 solution containing 100 μg/ml thermolysin (Sigma) at 64°C for ≈16 h ( ).

    Article Title: A new picture of cell wall protein dynamics in elongating cells of Arabidopsis thaliana: Confirmed actors and newcomers
    Article Snippet: .. Proteins were extracted from the cell wall fraction in two successive steps, first with a CaCl2 solution (5 mM sodium acetate buffer, pH 4.6, 0.2 M CaCl2 and 10 μL protease inhibitor cocktail, Sigma), followed by two extractions with a LiCl solution (5 mM sodium acetate buffer, pH 4.6, 2 M LiCl and 10 μL protease inhibitor cocktail). .. Protein separation by cation exchange chromatography Lyophilized proteins were dissolved in a total volume of 2 mL of water.

    Transferring:

    Article Title: Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage
    Article Snippet: .. The cell suspension was then added dropwise into 102 mmol/l CaCl2 solution (5 mmol/l HEPES, pH 7.4; Sigma-Aldrich) using a 5-ml pipette, allowing the condensed beads to gather in the CaCl2 solution for ~10 min so that each condensed bead would contain ~10,000 BMSCs. .. A five-fold NaCl solution (0.15 mol/l) was used to wash the suspension three times; the beads were then inoculated into six 75-cm2 culture flasks and cultured at 37°C with 5% CO2 .

    Cell Culture:

    Article Title: Mesenchymal stromal cells lower platelet activation and assist in platelet formation in vitro
    Article Snippet: Cells differentiated into megakaryocytes were cultured in media containing TPO only. .. To obtain a single cell suspension for subsequent cell sorting or flow cytometry analysis, artery segments were sequentially digested for 1.5 hours with PBS containing collagenase type 1 (562 U/ml; Invitrogen) and hyaluronidase (1 mg/ml; Thermo Fisher Scientific) and were supplemented with 1 M CaCl2 solution (MilliporeSigma) ( ).

    Article Title: Dynamic Hydrostatic Pressure Regulates Nucleus Pulposus Phenotypic Expression and Metabolism in a Cell Density-Dependent Manner
    Article Snippet: Nucleus pulposus cells were resuspended in 1.2% w/v alginate solution (Sigma Aldrich, St. Louis, MO) at a density of either 1 M/mL or 4 M/mL and dispensed in 102 mM CaCl2 solution (Sigma Aldrich) via a single-needle infusion pump (Cole-Parmer, Vernon Hills, IL) at a constant flow rate of 6 mL/h through a 21G needle and syringe to yield ∼30 beads/mL of alginate solution. .. Beads were cultured in complete media for 24 h and were then moved to multiwell tissue culture plates for loading studies.

    Article Title: Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage
    Article Snippet: The cell suspension was then added dropwise into 102 mmol/l CaCl2 solution (5 mmol/l HEPES, pH 7.4; Sigma-Aldrich) using a 5-ml pipette, allowing the condensed beads to gather in the CaCl2 solution for ~10 min so that each condensed bead would contain ~10,000 BMSCs. .. A five-fold NaCl solution (0.15 mol/l) was used to wash the suspension three times; the beads were then inoculated into six 75-cm2 culture flasks and cultured at 37°C with 5% CO2 .

    Generated:

    Article Title: A Novel In Vitro Model for Microvasculature Reveals Regulation of Circumferential ECM Organization by Curvature
    Article Snippet: Preparation of 3D fibrin hydrogel microfiber Fibrin hydrogel microfibers were generated by a new electrostretching method ( ) . .. The fibrinogen-alginate solution jet was collected at a distance of 3–5 cm from the needle tip, in a grounded, rotating bath (20 cm diameter, 20–40 rotation/min) containing 50 mM CaCl2 solution with 5 units/ml thrombin (Sigma-Aldrich).

    Article Title: Fabrication of 3-dimensional multicellular microvascular structures
    Article Snippet: Fibrin hydrogel microfibers were generated by the electrospun hydrogel method described previously ( ). .. The solution jet was collected in a grounded, rotating bath (30–45 rotations per minute) containing 50 mM CaCl2 solution with 10 U/ml thrombin (Sigma-Aldrich) for 35 minutes.

    Polymerase Chain Reaction:

    Article Title: Uncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen
    Article Snippet: The resulting GFP-puromycin fusion construct was amplified by PCR using forward 5′-GCCCTACGCGTATGGTGAGCAAGGGC-3′ and reverse 5′-GCCCTACGCGTGGCACCGGGCTTGC-3′ primers and ligated into the MluI site of pWNVIIRep. .. Plasmid DNA was mixed with H2 O and 1 M CaCl2 solution (Sigma-Aldrich, St. Louis, MO, USA) and then an equal volume of 2x HEPES-buffered saline (pH 7.1, 140 mM NaCl (Sigma-Aldrich, St. Louis, MO, USA), 1.5 mM Na2 HPO4 (Sigma-Aldrich, St. Louis, MO, USA) and 50 mM HEPES (Sigma-Aldrich, St. Louis, MO, USA) was added to the mixture drop-wise with simultaneous vortexing.

    Injection:

    Article Title: Dynamic assembly of liquid crystalline graphene oxide gel fibers for ion transport
    Article Snippet: In a typical spinning process, the GOLC dispersion was ejected into a coagulation bath containing 5 wt % CaCl2 solution with 0.25 M NH4 OH (30% NH3 solution, Sigma-Aldrich) at a constant rate (v 1 ) of 17 mm s−1 . .. The coagulated gel fiber was drawn at a constant rate (v 2 ) by controlling the revolutions per minute of a take-up roller. v 2 was set to 68 mm s−1 for obtaining 4v 1 -GO gel fibers. v 1 -GO gel fibers mean that the fibers are assembled by only the injection force of GO dispersion without the aid of the roller.

    Article Title: Alcohol diluent provides the optimal formulation for calcium chloride non-surgical sterilization in dogs
    Article Snippet: .. Preparation and intratesticular injection of CaCl2 solution To prepare the solution containing 20% CaCl2 and 1% lidocaine, 20 g of CaCl2 dihydrate powder (Sigma Aldrich Corporation) was added to a final volume of 100 mL with a 1% solution of lidocaine chlorhydrate (Salp spa, Italy), mixed, and sterilized in Falcon tubes. .. The alcohol solution of 20% CaCl2 dihydrate was prepared as follows: 20 g of CaCl2 dihydrate powder (Sigma Aldrich Corporation) was brought to a final volume of 100 mL of 95% ethanol (Baker Analyzed ACS, JT Baker), mixed, and sterilized in Falcon tubes.

    Molecular Weight:

    Article Title: Fabrication of 3-dimensional multicellular microvascular structures
    Article Snippet: Both solutions were dissolved in 0.2 weight-percent polyethylene oxide (molecular weight, 4 million; Sigma-Aldrich) before electrospinning. .. The solution jet was collected in a grounded, rotating bath (30–45 rotations per minute) containing 50 mM CaCl2 solution with 10 U/ml thrombin (Sigma-Aldrich) for 35 minutes.

    Fluorescence:

    Article Title: Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage
    Article Snippet: The cellular morphological changes and proliferation conditions were observed under an inverted fluorescence phase contrast microscope (Olympus Corp., Tokyo, Japan). .. The cell suspension was then added dropwise into 102 mmol/l CaCl2 solution (5 mmol/l HEPES, pH 7.4; Sigma-Aldrich) using a 5-ml pipette, allowing the condensed beads to gather in the CaCl2 solution for ~10 min so that each condensed bead would contain ~10,000 BMSCs.

    Transfection:

    Article Title: Uncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen
    Article Snippet: One to two hours prior transfection, the cells’ medium was changed with low-glucose DMEM medium (Life Technologies, Carlsbad, CA, USA) supplemented with 10% FBS and 1% P/S. .. Plasmid DNA was mixed with H2 O and 1 M CaCl2 solution (Sigma-Aldrich, St. Louis, MO, USA) and then an equal volume of 2x HEPES-buffered saline (pH 7.1, 140 mM NaCl (Sigma-Aldrich, St. Louis, MO, USA), 1.5 mM Na2 HPO4 (Sigma-Aldrich, St. Louis, MO, USA) and 50 mM HEPES (Sigma-Aldrich, St. Louis, MO, USA) was added to the mixture drop-wise with simultaneous vortexing.

    Labeling:

    Article Title: Chitosan Enriched Three-Dimensional Matrix Reduces Inflammatory and Catabolic Mediators Production by Human Chondrocytes
    Article Snippet: This mixture was slowly passed dropwise through a syringe into a 102 mM CaCl2 solution (Sigma). .. To visualize the chitosan distribution, some beads were prepared using similar chitosan labeled with fluorescein isothiocyanate (chitosan-FITC; generously provided by KitoZyme).

    Purification:

    Article Title: Localization of a bacterial protein in starch granules of transgenic maize kernels
    Article Snippet: .. To remove exogenous proteins from purified starch or ground maize kernel, samples were incubated with 5 mM CaCl2 solution containing 100 μg/ml thermolysin (Sigma) at 64°C for ≈16 h ( ). ..

    Sequencing:

    Article Title: Uncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen
    Article Snippet: The codon-optimized sequence the YFV structural genes was synthesized and cloned in pcDNA3.1(+) vector (Life Technologies, Carlsbad, CA, USA). .. Plasmid DNA was mixed with H2 O and 1 M CaCl2 solution (Sigma-Aldrich, St. Louis, MO, USA) and then an equal volume of 2x HEPES-buffered saline (pH 7.1, 140 mM NaCl (Sigma-Aldrich, St. Louis, MO, USA), 1.5 mM Na2 HPO4 (Sigma-Aldrich, St. Louis, MO, USA) and 50 mM HEPES (Sigma-Aldrich, St. Louis, MO, USA) was added to the mixture drop-wise with simultaneous vortexing.

    Protein Extraction:

    Article Title: A new picture of cell wall protein dynamics in elongating cells of Arabidopsis thaliana: Confirmed actors and newcomers
    Article Snippet: Paragraph title: Preparation of a cell wall fraction and protein extraction ... Proteins were extracted from the cell wall fraction in two successive steps, first with a CaCl2 solution (5 mM sodium acetate buffer, pH 4.6, 0.2 M CaCl2 and 10 μL protease inhibitor cocktail, Sigma), followed by two extractions with a LiCl solution (5 mM sodium acetate buffer, pH 4.6, 2 M LiCl and 10 μL protease inhibitor cocktail).

    Coagulation:

    Article Title: Dynamic assembly of liquid crystalline graphene oxide gel fibers for ion transport
    Article Snippet: .. In a typical spinning process, the GOLC dispersion was ejected into a coagulation bath containing 5 wt % CaCl2 solution with 0.25 M NH4 OH (30% NH3 solution, Sigma-Aldrich) at a constant rate (v 1 ) of 17 mm s−1 . .. The coagulated gel fiber was drawn at a constant rate (v 2 ) by controlling the revolutions per minute of a take-up roller. v 2 was set to 68 mm s−1 for obtaining 4v 1 -GO gel fibers. v 1 -GO gel fibers mean that the fibers are assembled by only the injection force of GO dispersion without the aid of the roller.

    Microscopy:

    Article Title: Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage
    Article Snippet: The cellular morphological changes and proliferation conditions were observed under an inverted fluorescence phase contrast microscope (Olympus Corp., Tokyo, Japan). .. The cell suspension was then added dropwise into 102 mmol/l CaCl2 solution (5 mmol/l HEPES, pH 7.4; Sigma-Aldrich) using a 5-ml pipette, allowing the condensed beads to gather in the CaCl2 solution for ~10 min so that each condensed bead would contain ~10,000 BMSCs.

    FACS:

    Article Title: Mesenchymal stromal cells lower platelet activation and assist in platelet formation in vitro
    Article Snippet: .. To obtain a single cell suspension for subsequent cell sorting or flow cytometry analysis, artery segments were sequentially digested for 1.5 hours with PBS containing collagenase type 1 (562 U/ml; Invitrogen) and hyaluronidase (1 mg/ml; Thermo Fisher Scientific) and were supplemented with 1 M CaCl2 solution (MilliporeSigma) ( ). .. Prior to cell culture, plates were coated with fibronectin (20 μg/ml; Stemcell Technologies) for 1 hour.

    Centrifugation:

    Article Title: Effect of activated autologous platelet-rich plasma on proliferation and osteogenic differentiation of human adipose-derived stem cells in vitro
    Article Snippet: After the second centrifugation at 200× g for 15 minutes, approximately 2 ml was plasma rich from platelets at bottom of Vacutainer and the upper rest was plasma poor from platelets. .. The PRP was activated with 10% CaCl2 solution (Sigma, St. Louis, MO, USA), 1 part CaCl2 stock solution was added to 9 parts PRP to mixing together.

    Titration:

    Article Title: Uncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen
    Article Snippet: Paragraph title: 2.3. Production and Titration of Reporter Virus Particles (RVPs) ... Plasmid DNA was mixed with H2 O and 1 M CaCl2 solution (Sigma-Aldrich, St. Louis, MO, USA) and then an equal volume of 2x HEPES-buffered saline (pH 7.1, 140 mM NaCl (Sigma-Aldrich, St. Louis, MO, USA), 1.5 mM Na2 HPO4 (Sigma-Aldrich, St. Louis, MO, USA) and 50 mM HEPES (Sigma-Aldrich, St. Louis, MO, USA) was added to the mixture drop-wise with simultaneous vortexing.

    Plasmid Preparation:

    Article Title: Uncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen
    Article Snippet: .. Plasmid DNA was mixed with H2 O and 1 M CaCl2 solution (Sigma-Aldrich, St. Louis, MO, USA) and then an equal volume of 2x HEPES-buffered saline (pH 7.1, 140 mM NaCl (Sigma-Aldrich, St. Louis, MO, USA), 1.5 mM Na2 HPO4 (Sigma-Aldrich, St. Louis, MO, USA) and 50 mM HEPES (Sigma-Aldrich, St. Louis, MO, USA) was added to the mixture drop-wise with simultaneous vortexing. .. The final concentration of CaCl2 in the transfection mix was 0.25 M. The resulting transfection mixture was incubated at room temperature for 20 min and then added to the cells.

    Produced:

    Article Title: Uncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen
    Article Snippet: RVPs were produced by transfecting HEK293T cells with pWNVIIRep-G/Puro and pcDNA3.1(+)-YFV CprME vectors at a molar ratio of 1:1 by the calcium phosphate method. .. Plasmid DNA was mixed with H2 O and 1 M CaCl2 solution (Sigma-Aldrich, St. Louis, MO, USA) and then an equal volume of 2x HEPES-buffered saline (pH 7.1, 140 mM NaCl (Sigma-Aldrich, St. Louis, MO, USA), 1.5 mM Na2 HPO4 (Sigma-Aldrich, St. Louis, MO, USA) and 50 mM HEPES (Sigma-Aldrich, St. Louis, MO, USA) was added to the mixture drop-wise with simultaneous vortexing.

    Concentration Assay:

    Article Title: Uncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen
    Article Snippet: Plasmid DNA was mixed with H2 O and 1 M CaCl2 solution (Sigma-Aldrich, St. Louis, MO, USA) and then an equal volume of 2x HEPES-buffered saline (pH 7.1, 140 mM NaCl (Sigma-Aldrich, St. Louis, MO, USA), 1.5 mM Na2 HPO4 (Sigma-Aldrich, St. Louis, MO, USA) and 50 mM HEPES (Sigma-Aldrich, St. Louis, MO, USA) was added to the mixture drop-wise with simultaneous vortexing. .. The final concentration of CaCl2 in the transfection mix was 0.25 M. The resulting transfection mixture was incubated at room temperature for 20 min and then added to the cells.

    Article Title: Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage
    Article Snippet: The cell pellets were suspended in low-viscosity sodium alginate (Sigma-Aldrich) solution (12.5 g/l) to a concentration of 2×105 cells/ml. .. The cell suspension was then added dropwise into 102 mmol/l CaCl2 solution (5 mmol/l HEPES, pH 7.4; Sigma-Aldrich) using a 5-ml pipette, allowing the condensed beads to gather in the CaCl2 solution for ~10 min so that each condensed bead would contain ~10,000 BMSCs.

    Staining:

    Article Title: Dynamic Hydrostatic Pressure Regulates Nucleus Pulposus Phenotypic Expression and Metabolism in a Cell Density-Dependent Manner
    Article Snippet: Nucleus pulposus cells were resuspended in 1.2% w/v alginate solution (Sigma Aldrich, St. Louis, MO) at a density of either 1 M/mL or 4 M/mL and dispensed in 102 mM CaCl2 solution (Sigma Aldrich) via a single-needle infusion pump (Cole-Parmer, Vernon Hills, IL) at a constant flow rate of 6 mL/h through a 21G needle and syringe to yield ∼30 beads/mL of alginate solution. .. For live/dead staining and adenosine triphosphate (ATP) measurements, we utilized 1 bead per well, and n = 3 wells per group.

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    Millipore affinity buffer a
    Affinity Buffer A, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore calpain activation solution
    Schematic representation of the proposed hypothetical cascade for DAergic vulnerability in PD. Given that environmental toxins, such as paraquat, interfere with calcium homeostasis via mitochondrial damage, the resulting increase in <t>calpain</t> activity may
    Calpain Activation Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore cacl2
    Schematic representation of the proposed hypothetical cascade for DAergic vulnerability in PD. Given that environmental toxins, such as paraquat, interfere with calcium homeostasis via mitochondrial damage, the resulting increase in <t>calpain</t> activity may
    Cacl2, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacl2/product/Millipore
    Average 99 stars, based on 50 article reviews
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    cacl2 - by Bioz Stars, 2020-02
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    Schematic representation of the proposed hypothetical cascade for DAergic vulnerability in PD. Given that environmental toxins, such as paraquat, interfere with calcium homeostasis via mitochondrial damage, the resulting increase in calpain activity may

    Journal: Acta neuropathologica

    Article Title: Environmental neurotoxic challenge of conditional alpha-synuclein transgenic mice predicts a dopaminergic olfactory-striatal interplay in early PD

    doi: 10.1007/s00401-014-1255-5

    Figure Lengend Snippet: Schematic representation of the proposed hypothetical cascade for DAergic vulnerability in PD. Given that environmental toxins, such as paraquat, interfere with calcium homeostasis via mitochondrial damage, the resulting increase in calpain activity may

    Article Snippet: For detection of calpain-resistant α-syn, free-floating sections were incubated in calpain activation solution (20 mM tris/HCl pH 7.8, 3.6 mM CaCl2 , 100 mM KCl2 ), containing 150 μg/ml calpain I (208712, Calbiochem) for 16 h at 37 °C.

    Techniques: Activity Assay

    Effect of paraquat on calpain activation and accumulation of CT α-syn. a , b OB protein extracts from non-transgenic (WT), A30P and paraquat-treated A30P+P and dox-treated control (A30P+D+P, A30P+D) mice were loaded on a SDS-PAGE or c native PAGE.

    Journal: Acta neuropathologica

    Article Title: Environmental neurotoxic challenge of conditional alpha-synuclein transgenic mice predicts a dopaminergic olfactory-striatal interplay in early PD

    doi: 10.1007/s00401-014-1255-5

    Figure Lengend Snippet: Effect of paraquat on calpain activation and accumulation of CT α-syn. a , b OB protein extracts from non-transgenic (WT), A30P and paraquat-treated A30P+P and dox-treated control (A30P+D+P, A30P+D) mice were loaded on a SDS-PAGE or c native PAGE.

    Article Snippet: For detection of calpain-resistant α-syn, free-floating sections were incubated in calpain activation solution (20 mM tris/HCl pH 7.8, 3.6 mM CaCl2 , 100 mM KCl2 ), containing 150 μg/ml calpain I (208712, Calbiochem) for 16 h at 37 °C.

    Techniques: Activation Assay, Transgenic Assay, Mouse Assay, SDS Page, Clear Native PAGE

    A30P+P mice show elevated levels of calpain-resistant α-syn in the GL. a , c Representative sections of GL from A30P and A30P+P mice with and without treatment of calpain-1 and, b , d quantitative results. a After calpain1 digestion, brain sections

    Journal: Acta neuropathologica

    Article Title: Environmental neurotoxic challenge of conditional alpha-synuclein transgenic mice predicts a dopaminergic olfactory-striatal interplay in early PD

    doi: 10.1007/s00401-014-1255-5

    Figure Lengend Snippet: A30P+P mice show elevated levels of calpain-resistant α-syn in the GL. a , c Representative sections of GL from A30P and A30P+P mice with and without treatment of calpain-1 and, b , d quantitative results. a After calpain1 digestion, brain sections

    Article Snippet: For detection of calpain-resistant α-syn, free-floating sections were incubated in calpain activation solution (20 mM tris/HCl pH 7.8, 3.6 mM CaCl2 , 100 mM KCl2 ), containing 150 μg/ml calpain I (208712, Calbiochem) for 16 h at 37 °C.

    Techniques: Mouse Assay