cacl2 powder  (Millipore)


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  • 88

    Structured Review

    Millipore cacl2 powder
    Cacl2 Powder, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacl2 powder/product/Millipore
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cacl2 powder - by Bioz Stars, 2020-02
    88/100 stars

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    Related Articles

    Functional Assay:

    Article Title: Mechanistic Insights of BHT-Mg-Catalyzed Ethylene Phosphate’s Coordination Ring-Opening Polymerization: DFT Modeling and Experimental Data
    Article Snippet: The B3PW91 functional was previously used to successfully model ROP [ , , , , , ]. .. CH2 Cl2 was washed with aqueous Na2 CO3 , stirred with CaCl2 powder, refluxed over CaH2 for 8 h and distilled. l -lactide and rac -lactide (Sigma-Aldrich/Merck, Darmstadt, Germany) were purified by recrystallization and subsequent sublimation. ε-Caprolactone was distilled prior to use under argon over CaH2 .

    Synthesized:

    Article Title: Mechanistic Insights of BHT-Mg-Catalyzed Ethylene Phosphate’s Coordination Ring-Opening Polymerization: DFT Modeling and Experimental Data
    Article Snippet: CH2 Cl2 was washed with aqueous Na2 CO3 , stirred with CaCl2 powder, refluxed over CaH2 for 8 h and distilled. l -lactide and rac -lactide (Sigma-Aldrich/Merck, Darmstadt, Germany) were purified by recrystallization and subsequent sublimation. ε-Caprolactone was distilled prior to use under argon over CaH2 . .. Methyl ethylene phosphate (MeOEP) [ ] and [(BHT)Mg(μ-OBn)(THF)]2 ( Mg1 ) [ ] were synthesized according to the literature procedures.

    Sublimation:

    Article Title: Mechanistic Insights of BHT-Mg-Catalyzed Ethylene Phosphate’s Coordination Ring-Opening Polymerization: DFT Modeling and Experimental Data
    Article Snippet: .. CH2 Cl2 was washed with aqueous Na2 CO3 , stirred with CaCl2 powder, refluxed over CaH2 for 8 h and distilled. l -lactide and rac -lactide (Sigma-Aldrich/Merck, Darmstadt, Germany) were purified by recrystallization and subsequent sublimation. ε-Caprolactone was distilled prior to use under argon over CaH2 . .. Methyl ethylene phosphate (MeOEP) [ ] and [(BHT)Mg(μ-OBn)(THF)]2 ( Mg1 ) [ ] were synthesized according to the literature procedures.

    Nuclear Magnetic Resonance:

    Article Title: Mechanistic Insights of BHT-Mg-Catalyzed Ethylene Phosphate’s Coordination Ring-Opening Polymerization: DFT Modeling and Experimental Data
    Article Snippet: CH2 Cl2 was washed with aqueous Na2 CO3 , stirred with CaCl2 powder, refluxed over CaH2 for 8 h and distilled. l -lactide and rac -lactide (Sigma-Aldrich/Merck, Darmstadt, Germany) were purified by recrystallization and subsequent sublimation. ε-Caprolactone was distilled prior to use under argon over CaH2 . .. The 1 H and 31 P NMR spectra were recorded on a Bruker AVANCE 400 spectrometer (400 MHz) (Bruker Corporation, Billerica, MA, USA) at 20 °C.

    Purification:

    Article Title: Mechanistic Insights of BHT-Mg-Catalyzed Ethylene Phosphate’s Coordination Ring-Opening Polymerization: DFT Modeling and Experimental Data
    Article Snippet: .. CH2 Cl2 was washed with aqueous Na2 CO3 , stirred with CaCl2 powder, refluxed over CaH2 for 8 h and distilled. l -lactide and rac -lactide (Sigma-Aldrich/Merck, Darmstadt, Germany) were purified by recrystallization and subsequent sublimation. ε-Caprolactone was distilled prior to use under argon over CaH2 . .. Methyl ethylene phosphate (MeOEP) [ ] and [(BHT)Mg(μ-OBn)(THF)]2 ( Mg1 ) [ ] were synthesized according to the literature procedures.

    Concentration Assay:

    Article Title: Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue
    Article Snippet: .. Crosslinking was obtained by diluting CaCl2 powder (Sigma Aldrich, Italy) in sterile distilled water at a final concentration of 2% and maintained for 5 min on the 3D printed structure. ..

    Article Title: Control of nacre biomineralization by Pif80 in pearl oyster
    Article Snippet: A CaCl2 aqueous solution was prepared by dissolving CaCl2 powder (Sigma-Aldrich) in DW. .. The Ca2+ concentration of the supersaturated CaCO3 solution was measured with QuantiChrom Calcium Assay Kit (BioAssay Systems).

    Calcium Assay:

    Article Title: Control of nacre biomineralization by Pif80 in pearl oyster
    Article Snippet: A CaCl2 aqueous solution was prepared by dissolving CaCl2 powder (Sigma-Aldrich) in DW. .. The Ca2+ concentration of the supersaturated CaCO3 solution was measured with QuantiChrom Calcium Assay Kit (BioAssay Systems).

    Recrystallization:

    Article Title: Mechanistic Insights of BHT-Mg-Catalyzed Ethylene Phosphate’s Coordination Ring-Opening Polymerization: DFT Modeling and Experimental Data
    Article Snippet: .. CH2 Cl2 was washed with aqueous Na2 CO3 , stirred with CaCl2 powder, refluxed over CaH2 for 8 h and distilled. l -lactide and rac -lactide (Sigma-Aldrich/Merck, Darmstadt, Germany) were purified by recrystallization and subsequent sublimation. ε-Caprolactone was distilled prior to use under argon over CaH2 . .. Methyl ethylene phosphate (MeOEP) [ ] and [(BHT)Mg(μ-OBn)(THF)]2 ( Mg1 ) [ ] were synthesized according to the literature procedures.

    Injection:

    Article Title: A dose-finding, long-term study on the use of calcium chloride in saline solution as a method of nonsurgical sterilization in dogs: evaluation of the most effective concentration with the lowest risk
    Article Snippet: Paragraph title: Preparation and intratesticular injection of CaCl2 solution ... For each experimental group, 10 g (Group A), 20 g (Group B), 30 g (Group C), or 60 g (Group D) of CaCl2 powder (Sigma Aldrich Corporation) was dissolved in a physiological saline solution (Sodio Cloruro Bieffe Medital 0.9%, Grosotto [SO], Italy) to a final volume of 100 ml, mixed, and sterilized in Falcon tubes.

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  • 79
    Millipore tppe digestion buffer
    <t>TPPE</t> cleaves the reduced pro-BTH6 in the thionin domain. LC-ESI-MS analysis of the digestion of <t>carboxymethylated</t> myc-proBTH6-strep with recombinant TPPE. The acidic domain is cleaved several times with additional sites compared with the oxidized proprotein.
    Tppe Digestion Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tppe digestion buffer/product/Millipore
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tppe digestion buffer - by Bioz Stars, 2020-02
    79/100 stars
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    79
    Millipore cytokine homogenate buffer
    Leptin and <t>cytokine</t> levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.
    Cytokine Homogenate Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokine homogenate buffer/product/Millipore
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cytokine homogenate buffer - by Bioz Stars, 2020-02
    79/100 stars
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    77
    Millipore glucose free artificial csf
    Native human Aß stimulated glucose uptake in neurons. (A) Summary of the cases used in this study. <t>CSF</t> samples were from the Department of Neurological and Psychiatric Sciences, University of Florence. Clinical diagnosis of AD was defined according to the Diagnostic and Statistical Manual of Mental Disorders criteria (DSM-IV). Clinical diagnosis of MCI was defined according to Petersen's validated criteria (Petersen et al., 2001 ). CSF Aß 1−42 was quantitated by INNOTEST ß-amyloid (1–42) from Innogenetics. (B) Contribution of CSF Aß monomers to depolarization-induced neuronal glucose uptake both in the absence (IgG2b isotype control) and in the presence (4G8) of a neutralizing anti-Aß antibody. Neurons were pre-exposed to the γ-secretase inhibitor IX and underwent a 15 min depolarization pulse with 400 mM <t>KCl</t> (γ-Sec Inh + KCl). Mean values of glucose uptake (mg/dl) after the pulse were the following: 47.75 ± 4.77 vs. 33.87 ± 4.54 * ( * different at p
    Glucose Free Artificial Csf, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glucose free artificial csf/product/Millipore
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glucose free artificial csf - by Bioz Stars, 2020-02
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    99
    Millipore cacl2
    Cross‐linking of fibrin by full fength rFXIIIA and fragments. Clots were formed with fibrinogen, thrombin, <t>CaCl2,</t> and either full length rFXIIIA (lane 2), fragment TB 1 (lacking barrel 2; lane 3), TCC (lacking barrels 1 and 2; lane 4), TBS (lacking catalytic core and barrels 1 and 2; lane 5), or a control without any form of FXIII (lane 6). After 30 min, samples were run on an SDS ‐ PAGE gel under reducing conditions (A). The mean density of the different bands was determined relative to the control for that peptide chain or cross‐linked structure (B). FXIIIA , full length FXIII A subunit; TB 1, full length FXIII A subunit lacking barrel 2; TBS , full length FXIII A subunit lacking barrels 1 and 2, and catalytic core; TCC , full length FXIII A subunit lacking barrels 1 and 2.
    Cacl2, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacl2/product/Millipore
    Average 99 stars, based on 50 article reviews
    Price from $9.99 to $1999.99
    cacl2 - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    Image Search Results


    TPPE cleaves the reduced pro-BTH6 in the thionin domain. LC-ESI-MS analysis of the digestion of carboxymethylated myc-proBTH6-strep with recombinant TPPE. The acidic domain is cleaved several times with additional sites compared with the oxidized proprotein.

    Journal: The Journal of Biological Chemistry

    Article Title: Isolation and Characterization of a Thionin Proprotein-processing Enzyme from Barley *

    doi: 10.1074/jbc.M115.647859

    Figure Lengend Snippet: TPPE cleaves the reduced pro-BTH6 in the thionin domain. LC-ESI-MS analysis of the digestion of carboxymethylated myc-proBTH6-strep with recombinant TPPE. The acidic domain is cleaved several times with additional sites compared with the oxidized proprotein.

    Article Snippet: The reduced and cysteine-carboxymethylated protein samples were dialyzed against TPPE digestion buffer (25 m m MES, 100 m m NaCl, 10 m m CaCl2 , pH 6.5) using Amicon Ultra 10K ultrafiltration centrifugal filters (Millipore) according to the recommendations of the manufacturer.

    Techniques: Mass Spectrometry, Recombinant

    Leptin and cytokine levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.

    Journal: BioMed Research International

    Article Title: Advanced Glycation End Products Induce Obesity and Hepatosteatosis in CD-1 Wild-Type Mice

    doi: 10.1155/2016/7867852

    Figure Lengend Snippet: Leptin and cytokine levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.

    Article Snippet: The remaining liver was washed with cold HBSS and suspended in cytokine homogenate buffer (150 mM NaCl, 15 mM Tris, 1 mM CaCl2 ·2H2 O, and 1 mM MgCl2 ·6H2 O, adjusted to pH 7.4) plus 100x protease inhibitor cocktail 1 (Calbiochem, La Jolla, California) to a total volume of 10 mL and homogenized on ice using a Polytron® Homogenizer (Kinematica Inc., Bohemia, NY).

    Techniques:

    Native human Aß stimulated glucose uptake in neurons. (A) Summary of the cases used in this study. CSF samples were from the Department of Neurological and Psychiatric Sciences, University of Florence. Clinical diagnosis of AD was defined according to the Diagnostic and Statistical Manual of Mental Disorders criteria (DSM-IV). Clinical diagnosis of MCI was defined according to Petersen's validated criteria (Petersen et al., 2001 ). CSF Aß 1−42 was quantitated by INNOTEST ß-amyloid (1–42) from Innogenetics. (B) Contribution of CSF Aß monomers to depolarization-induced neuronal glucose uptake both in the absence (IgG2b isotype control) and in the presence (4G8) of a neutralizing anti-Aß antibody. Neurons were pre-exposed to the γ-secretase inhibitor IX and underwent a 15 min depolarization pulse with 400 mM KCl (γ-Sec Inh + KCl). Mean values of glucose uptake (mg/dl) after the pulse were the following: 47.75 ± 4.77 vs. 33.87 ± 4.54 * ( * different at p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Monomeric ß-amyloid interacts with type-1 insulin-like growth factor receptors to provide energy supply to neurons

    doi: 10.3389/fncel.2015.00297

    Figure Lengend Snippet: Native human Aß stimulated glucose uptake in neurons. (A) Summary of the cases used in this study. CSF samples were from the Department of Neurological and Psychiatric Sciences, University of Florence. Clinical diagnosis of AD was defined according to the Diagnostic and Statistical Manual of Mental Disorders criteria (DSM-IV). Clinical diagnosis of MCI was defined according to Petersen's validated criteria (Petersen et al., 2001 ). CSF Aß 1−42 was quantitated by INNOTEST ß-amyloid (1–42) from Innogenetics. (B) Contribution of CSF Aß monomers to depolarization-induced neuronal glucose uptake both in the absence (IgG2b isotype control) and in the presence (4G8) of a neutralizing anti-Aß antibody. Neurons were pre-exposed to the γ-secretase inhibitor IX and underwent a 15 min depolarization pulse with 400 mM KCl (γ-Sec Inh + KCl). Mean values of glucose uptake (mg/dl) after the pulse were the following: 47.75 ± 4.77 vs. 33.87 ± 4.54 * ( * different at p

    Article Snippet: For the experiment, cultures were rinsed in glucose-free artificial CSF (124 mM NaCl, 2.5 mM KCl, 2 mM MgSO4 , 1.25 mM KH2 PO4 , 26 mM NaHCO3, 2.5 mM CaCl2 ) and maintained for 45 min under glucose deprivation. γ-Sec-inhibitor IX (Calbiochem, 100 nM) was added 2 h before glucose deprivation and maintained troughout the experiment.

    Techniques: Diagnostic Assay, Size-exclusion Chromatography

    Cross‐linking of fibrin by full fength rFXIIIA and fragments. Clots were formed with fibrinogen, thrombin, CaCl2, and either full length rFXIIIA (lane 2), fragment TB 1 (lacking barrel 2; lane 3), TCC (lacking barrels 1 and 2; lane 4), TBS (lacking catalytic core and barrels 1 and 2; lane 5), or a control without any form of FXIII (lane 6). After 30 min, samples were run on an SDS ‐ PAGE gel under reducing conditions (A). The mean density of the different bands was determined relative to the control for that peptide chain or cross‐linked structure (B). FXIIIA , full length FXIII A subunit; TB 1, full length FXIII A subunit lacking barrel 2; TBS , full length FXIII A subunit lacking barrels 1 and 2, and catalytic core; TCC , full length FXIII A subunit lacking barrels 1 and 2.

    Journal: Journal of Thrombosis and Haemostasis

    Article Title: The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit. The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit

    doi: 10.1111/jth.14128

    Figure Lengend Snippet: Cross‐linking of fibrin by full fength rFXIIIA and fragments. Clots were formed with fibrinogen, thrombin, CaCl2, and either full length rFXIIIA (lane 2), fragment TB 1 (lacking barrel 2; lane 3), TCC (lacking barrels 1 and 2; lane 4), TBS (lacking catalytic core and barrels 1 and 2; lane 5), or a control without any form of FXIII (lane 6). After 30 min, samples were run on an SDS ‐ PAGE gel under reducing conditions (A). The mean density of the different bands was determined relative to the control for that peptide chain or cross‐linked structure (B). FXIIIA , full length FXIII A subunit; TB 1, full length FXIII A subunit lacking barrel 2; TBS , full length FXIII A subunit lacking barrels 1 and 2, and catalytic core; TCC , full length FXIII A subunit lacking barrels 1 and 2.

    Article Snippet: Briefly, microtiter plates were coated with 40 μg mL−1 human fibrinogen (Enzyme Research Laboratories) at 37 °C for 1 h. After blocking with 1% BSA, plates were incubated with 1 U mL−1 human thrombin (Calbiochem) and 5 mm CaCl2 to convert fibrinogen to fibrin, and then treated in triplicate with 3.5 nm recombinant FXIII‐A sample, 10 μg mL−1 α2 ‐antiplasmin (Calbiochem), 1 U mL−1 human thrombin, 0.1 mm DTT, and 1 mm CaCl2 .

    Techniques: SDS Page

    Effect of recombinant Factor XIIIA and fragments on turbidity and clot lysis. Polymerisation of FXIIIA depleted fibrinogen was initiated by the addition of thrombin, CaCl2, and either full length rFXIIIA (black square), fragment TB 1 (lacking barrel 2; dark grey diamond), TCC (lacking barrels 1 and 2; mid‐grey triangle), TBS (lacking catalytic core and barrels 1 and 2; light grey circles), or a control (buffer; very light grey crosses). Generation of turbidity was measured every 12 s for 60 min and results shown are mean values of triplicate experiments (A). Only clots with full‐length FXIII ‐A had a significant decrease in final maximum absorbance compared to control ( n = 3, P

    Journal: Journal of Thrombosis and Haemostasis

    Article Title: The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit. The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit

    doi: 10.1111/jth.14128

    Figure Lengend Snippet: Effect of recombinant Factor XIIIA and fragments on turbidity and clot lysis. Polymerisation of FXIIIA depleted fibrinogen was initiated by the addition of thrombin, CaCl2, and either full length rFXIIIA (black square), fragment TB 1 (lacking barrel 2; dark grey diamond), TCC (lacking barrels 1 and 2; mid‐grey triangle), TBS (lacking catalytic core and barrels 1 and 2; light grey circles), or a control (buffer; very light grey crosses). Generation of turbidity was measured every 12 s for 60 min and results shown are mean values of triplicate experiments (A). Only clots with full‐length FXIII ‐A had a significant decrease in final maximum absorbance compared to control ( n = 3, P

    Article Snippet: Briefly, microtiter plates were coated with 40 μg mL−1 human fibrinogen (Enzyme Research Laboratories) at 37 °C for 1 h. After blocking with 1% BSA, plates were incubated with 1 U mL−1 human thrombin (Calbiochem) and 5 mm CaCl2 to convert fibrinogen to fibrin, and then treated in triplicate with 3.5 nm recombinant FXIII‐A sample, 10 μg mL−1 α2 ‐antiplasmin (Calbiochem), 1 U mL−1 human thrombin, 0.1 mm DTT, and 1 mm CaCl2 .

    Techniques: Recombinant, Lysis