cacl2 acidic phenol chloroform isoamyl alcohol  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cacl2 acidic phenol chloroform isoamyl alcohol
    Cacl2 Acidic Phenol Chloroform Isoamyl Alcohol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacl2 acidic phenol chloroform isoamyl alcohol/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cacl2 acidic phenol chloroform isoamyl alcohol - by Bioz Stars, 2020-09
    86/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: mRNA Display Using Covalent Coupling of mRNA to Translated Proteins
    Article Snippet: .. Expand long template PCR system (Roche) T7 RNA polymerase (NEB) 10× RNA polymerase buffer: 400 mM, Tris-HCl, pH7.9, 60 mM MgCl2 , 100 mM DTT, 20 mM spermidine RNase-free DNase (Promega) 10× DNase buffer: 400 mM Tris-HCl, pH8.0, 100 mM MgSO4 , 10 mM CaCl2 Acidic phenol chloroform : isoamyl alcohol (Ambion) 7.5 M LiCl RNA precipitation solution (Ambion) Puromycin oligo linker: 5′-Psoralen-(TAGCCGGTG)2′-OMe -dA15 C9C9dAdCdC-Puromycin-3′ Retic lysate IVT™ kit (Ambion) [35 S]-L-methionine (Perkin Elmer) Oligo(dT) cellulose (Ambion) 1× Oligo(dT) binding buffer: 100 mM Tris-HCl, pH 8.0, 1 M NaCl, 10 mM EDTA, 0.2% Triton X-100 Oligo(dT) wash buffer: 20 mM Tris-HCl, pH 8.0, 300 mM KCl, 0.1% Tween-20 RNase-free 10 mL poly-prep chromatography column (Biorad) SuperScript II RNase H− reverse transcriptase (Invitrogen) Reverse transcription primer: TTTTTTTTTTNNCCAGATCCAGACATTCCCAT Anti-FLAG M2 affinity gel (Sigma) FLAG peptide (Sigma) 100 mM glycine buffer pH3.5 1× TBST buffer: 20 mM Tris-HCl, pH8.0, 150 mM NaCl, 0.2 % Tween-20 1× TE buffer: 10 mM Tris-HCl, pH8.0, 1 mM EDTA NAP-5 column (GE Healthcare) NAP-10 column (GE Healthcare) QIAquick PCR purification kit (Qiagen) QIAquick gel extraction kit (Qiagen) .. Thermal cycler Nanodrop spectrophotometer UV lamp (Black Ray Lamp 365 nm, 0.16 Amps) Barnstead labquake shaker/rotator Scintillation counter

    Gel Extraction:

    Article Title: mRNA Display Using Covalent Coupling of mRNA to Translated Proteins
    Article Snippet: .. Expand long template PCR system (Roche) T7 RNA polymerase (NEB) 10× RNA polymerase buffer: 400 mM, Tris-HCl, pH7.9, 60 mM MgCl2 , 100 mM DTT, 20 mM spermidine RNase-free DNase (Promega) 10× DNase buffer: 400 mM Tris-HCl, pH8.0, 100 mM MgSO4 , 10 mM CaCl2 Acidic phenol chloroform : isoamyl alcohol (Ambion) 7.5 M LiCl RNA precipitation solution (Ambion) Puromycin oligo linker: 5′-Psoralen-(TAGCCGGTG)2′-OMe -dA15 C9C9dAdCdC-Puromycin-3′ Retic lysate IVT™ kit (Ambion) [35 S]-L-methionine (Perkin Elmer) Oligo(dT) cellulose (Ambion) 1× Oligo(dT) binding buffer: 100 mM Tris-HCl, pH 8.0, 1 M NaCl, 10 mM EDTA, 0.2% Triton X-100 Oligo(dT) wash buffer: 20 mM Tris-HCl, pH 8.0, 300 mM KCl, 0.1% Tween-20 RNase-free 10 mL poly-prep chromatography column (Biorad) SuperScript II RNase H− reverse transcriptase (Invitrogen) Reverse transcription primer: TTTTTTTTTTNNCCAGATCCAGACATTCCCAT Anti-FLAG M2 affinity gel (Sigma) FLAG peptide (Sigma) 100 mM glycine buffer pH3.5 1× TBST buffer: 20 mM Tris-HCl, pH8.0, 150 mM NaCl, 0.2 % Tween-20 1× TE buffer: 10 mM Tris-HCl, pH8.0, 1 mM EDTA NAP-5 column (GE Healthcare) NAP-10 column (GE Healthcare) QIAquick PCR purification kit (Qiagen) QIAquick gel extraction kit (Qiagen) .. Thermal cycler Nanodrop spectrophotometer UV lamp (Black Ray Lamp 365 nm, 0.16 Amps) Barnstead labquake shaker/rotator Scintillation counter

    Binding Assay:

    Article Title: mRNA Display Using Covalent Coupling of mRNA to Translated Proteins
    Article Snippet: .. Expand long template PCR system (Roche) T7 RNA polymerase (NEB) 10× RNA polymerase buffer: 400 mM, Tris-HCl, pH7.9, 60 mM MgCl2 , 100 mM DTT, 20 mM spermidine RNase-free DNase (Promega) 10× DNase buffer: 400 mM Tris-HCl, pH8.0, 100 mM MgSO4 , 10 mM CaCl2 Acidic phenol chloroform : isoamyl alcohol (Ambion) 7.5 M LiCl RNA precipitation solution (Ambion) Puromycin oligo linker: 5′-Psoralen-(TAGCCGGTG)2′-OMe -dA15 C9C9dAdCdC-Puromycin-3′ Retic lysate IVT™ kit (Ambion) [35 S]-L-methionine (Perkin Elmer) Oligo(dT) cellulose (Ambion) 1× Oligo(dT) binding buffer: 100 mM Tris-HCl, pH 8.0, 1 M NaCl, 10 mM EDTA, 0.2% Triton X-100 Oligo(dT) wash buffer: 20 mM Tris-HCl, pH 8.0, 300 mM KCl, 0.1% Tween-20 RNase-free 10 mL poly-prep chromatography column (Biorad) SuperScript II RNase H− reverse transcriptase (Invitrogen) Reverse transcription primer: TTTTTTTTTTNNCCAGATCCAGACATTCCCAT Anti-FLAG M2 affinity gel (Sigma) FLAG peptide (Sigma) 100 mM glycine buffer pH3.5 1× TBST buffer: 20 mM Tris-HCl, pH8.0, 150 mM NaCl, 0.2 % Tween-20 1× TE buffer: 10 mM Tris-HCl, pH8.0, 1 mM EDTA NAP-5 column (GE Healthcare) NAP-10 column (GE Healthcare) QIAquick PCR purification kit (Qiagen) QIAquick gel extraction kit (Qiagen) .. Thermal cycler Nanodrop spectrophotometer UV lamp (Black Ray Lamp 365 nm, 0.16 Amps) Barnstead labquake shaker/rotator Scintillation counter

    Chromatography:

    Article Title: mRNA Display Using Covalent Coupling of mRNA to Translated Proteins
    Article Snippet: .. Expand long template PCR system (Roche) T7 RNA polymerase (NEB) 10× RNA polymerase buffer: 400 mM, Tris-HCl, pH7.9, 60 mM MgCl2 , 100 mM DTT, 20 mM spermidine RNase-free DNase (Promega) 10× DNase buffer: 400 mM Tris-HCl, pH8.0, 100 mM MgSO4 , 10 mM CaCl2 Acidic phenol chloroform : isoamyl alcohol (Ambion) 7.5 M LiCl RNA precipitation solution (Ambion) Puromycin oligo linker: 5′-Psoralen-(TAGCCGGTG)2′-OMe -dA15 C9C9dAdCdC-Puromycin-3′ Retic lysate IVT™ kit (Ambion) [35 S]-L-methionine (Perkin Elmer) Oligo(dT) cellulose (Ambion) 1× Oligo(dT) binding buffer: 100 mM Tris-HCl, pH 8.0, 1 M NaCl, 10 mM EDTA, 0.2% Triton X-100 Oligo(dT) wash buffer: 20 mM Tris-HCl, pH 8.0, 300 mM KCl, 0.1% Tween-20 RNase-free 10 mL poly-prep chromatography column (Biorad) SuperScript II RNase H− reverse transcriptase (Invitrogen) Reverse transcription primer: TTTTTTTTTTNNCCAGATCCAGACATTCCCAT Anti-FLAG M2 affinity gel (Sigma) FLAG peptide (Sigma) 100 mM glycine buffer pH3.5 1× TBST buffer: 20 mM Tris-HCl, pH8.0, 150 mM NaCl, 0.2 % Tween-20 1× TE buffer: 10 mM Tris-HCl, pH8.0, 1 mM EDTA NAP-5 column (GE Healthcare) NAP-10 column (GE Healthcare) QIAquick PCR purification kit (Qiagen) QIAquick gel extraction kit (Qiagen) .. Thermal cycler Nanodrop spectrophotometer UV lamp (Black Ray Lamp 365 nm, 0.16 Amps) Barnstead labquake shaker/rotator Scintillation counter

    Purification:

    Article Title: mRNA Display Using Covalent Coupling of mRNA to Translated Proteins
    Article Snippet: .. Expand long template PCR system (Roche) T7 RNA polymerase (NEB) 10× RNA polymerase buffer: 400 mM, Tris-HCl, pH7.9, 60 mM MgCl2 , 100 mM DTT, 20 mM spermidine RNase-free DNase (Promega) 10× DNase buffer: 400 mM Tris-HCl, pH8.0, 100 mM MgSO4 , 10 mM CaCl2 Acidic phenol chloroform : isoamyl alcohol (Ambion) 7.5 M LiCl RNA precipitation solution (Ambion) Puromycin oligo linker: 5′-Psoralen-(TAGCCGGTG)2′-OMe -dA15 C9C9dAdCdC-Puromycin-3′ Retic lysate IVT™ kit (Ambion) [35 S]-L-methionine (Perkin Elmer) Oligo(dT) cellulose (Ambion) 1× Oligo(dT) binding buffer: 100 mM Tris-HCl, pH 8.0, 1 M NaCl, 10 mM EDTA, 0.2% Triton X-100 Oligo(dT) wash buffer: 20 mM Tris-HCl, pH 8.0, 300 mM KCl, 0.1% Tween-20 RNase-free 10 mL poly-prep chromatography column (Biorad) SuperScript II RNase H− reverse transcriptase (Invitrogen) Reverse transcription primer: TTTTTTTTTTNNCCAGATCCAGACATTCCCAT Anti-FLAG M2 affinity gel (Sigma) FLAG peptide (Sigma) 100 mM glycine buffer pH3.5 1× TBST buffer: 20 mM Tris-HCl, pH8.0, 150 mM NaCl, 0.2 % Tween-20 1× TE buffer: 10 mM Tris-HCl, pH8.0, 1 mM EDTA NAP-5 column (GE Healthcare) NAP-10 column (GE Healthcare) QIAquick PCR purification kit (Qiagen) QIAquick gel extraction kit (Qiagen) .. Thermal cycler Nanodrop spectrophotometer UV lamp (Black Ray Lamp 365 nm, 0.16 Amps) Barnstead labquake shaker/rotator Scintillation counter

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    Thermo Fisher annexin binding buffer
    mTOR inhibition protects from methotrexate and 6-mercaptopurine (A) BV173 and (B) SUP-B15 Ph + B-ALL cells are treated with MTX and 6MP for 48 hours in presence of 100 nM MLN0128. Viability is measured by <t>Annexin-V</t> and PI staining. Unpaired t-tests of were performed, n=3, mean±SD, *p
    Annexin Binding Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 368 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin binding buffer/product/Thermo Fisher
    Average 99 stars, based on 368 article reviews
    Price from $9.99 to $1999.99
    annexin binding buffer - by Bioz Stars, 2020-09
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    92
    Thermo Fisher csf xb buffer
    Characterization of pSer335 <t>XErp1</t> antibody <t>CSF</t> extract was treated with Myc‐XErp1 IVT carrying the indicated combinations of the mutations DSG − (S33N S38N), DSA − (S284N S288N), ZBR − (C583A) and CaMKII − (T195A). Calcium was added, samples were taken at the indicated time points and as indicated treated with λ‐phosphatase. Samples were immunoblotted for the Myc‐tag and cyclin B2. The cyclin B2 membrane was stripped and reprobed for α‐tubulin. Asterisk indicates unspecific bands. Several lanes were removed at the dashed line. CSF extract was treated with Myc‐XErp1 CaMKII − ZBR − (T195A C583A) IVT at the indicated dilutions. An empty IVT not expressing XErp1 and an untreated condition were used as controls. Samples were taken, treated as indicated with λ‐phosphatase and immunoblotted for XErp1, pSer335 XErp1, and α‐tubulin. The XErp1 membrane was stripped and reprobed for the Myc‐tag. Asterisks indicate unspecific bands. CSF extract was treated with Myc‐XErp1 CaMKII − ZBR − (T195A C583A) IVT that was either wild‐type or mutated to alanine at Ser335. An empty IVT reaction not expressing Myc‐XErp1 served as control. Samples were taken, treated as indicated with λ‐phosphatase and immunoblotted for XErp1, the Myc‐tag, pSer335 XErp1, and α‐tubulin. Asterisks indicate unspecific bands. Source data are available online for this figure.
    Csf Xb Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/csf xb buffer/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
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    88
    Thermo Fisher anxa5 binding buffer
    Autophagy-deficiency during myoblast differentiation augments apoptotic signaling, ER-stress responses, and cell death. Quantitative analysis of early apoptotic <t>(ANXA5</t> + PI − ) cells ( a ), late apoptotic (ANXA5 + PI + ) cells ( b ), and CASP3 activity ( c ) in SCR and sh Atg7 cells during differentiation. Representative immunoblots ( d ) and quantitative analysis ( e, f, g, h ) of BAX, BCL2, and HSPA (as well as calculated BAX:BCL2 ratio) in SCR and sh Atg7 cells during differentiation. Quantitative analysis of CAPN activity ( i ) in SCR and sh Atg7 cells during differentiation. *p
    Anxa5 Binding Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anxa5 binding buffer/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anxa5 binding buffer - by Bioz Stars, 2020-09
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    Image Search Results


    mTOR inhibition protects from methotrexate and 6-mercaptopurine (A) BV173 and (B) SUP-B15 Ph + B-ALL cells are treated with MTX and 6MP for 48 hours in presence of 100 nM MLN0128. Viability is measured by Annexin-V and PI staining. Unpaired t-tests of were performed, n=3, mean±SD, *p

    Journal: Molecular cancer therapeutics

    Article Title: mTORC1 Inhibition Induces Resistance to Methotrexate and 6-Mercaptopurine in Ph+ and Ph-like B-ALL

    doi: 10.1158/1535-7163.MCT-17-0024

    Figure Lengend Snippet: mTOR inhibition protects from methotrexate and 6-mercaptopurine (A) BV173 and (B) SUP-B15 Ph + B-ALL cells are treated with MTX and 6MP for 48 hours in presence of 100 nM MLN0128. Viability is measured by Annexin-V and PI staining. Unpaired t-tests of were performed, n=3, mean±SD, *p

    Article Snippet: For harvesting, both cell lines and patient samples were pelleted, resuspended in 150 μL of Annexin Binding Buffer (10 mM HEPES, 140 mM NaCl and 2.5 mM CaCl2 -2H2 O, pH 7.4) containing Annexin-V Alexa Fluor 647 (ThermoFisher) and 0.4 μg/mL propidium iodide (ThermoFisher).

    Techniques: Inhibition, Staining

    ( a ) HRV16 replication is down regulated with inhibition of caspase 8. O-HeLa cells were infected with HRV16 at an M.O.I of 3. At 3 h.p.i., ActoD (5 µg/mL) was added to selected samples. Caspase 8 was inhibited as indicated through addition caspase 8 inhibitor (4 µM) at 4 h.p.i. Cultures were frozen at 24 h.p.i. Once thawed, virus was clarified and titrated. Results are expressed as mean of 3 independent experiments. Error bars are +/− standard error of the mean. *p ≤ 0.05, ns – nonsignificant. ( b ) HRV16 restricts apoptotic pathways. O-HeLa cells were infected and treated with ActoD as in a) above. Cells were collected at 12 h.p.i and the effect of treatment and/or infection analysed by flow cytometry after staining with annexin V-FITC (An) and propidium iodide (Pi). Representative dual-parameter fluorescence density blots were acquired. ( c ) Bars are representative of percentage of viable (An−/Pi−), early apoptotic (An+/Pi−), late apoptotic (An+/Pi+) or necrotic (An−/Pi+) cells of total number of cells within each sample.

    Journal: Scientific Reports

    Article Title: Human Rhinovirus 3C protease cleaves RIPK1, concurrent with caspase 8 activation

    doi: 10.1038/s41598-018-19839-4

    Figure Lengend Snippet: ( a ) HRV16 replication is down regulated with inhibition of caspase 8. O-HeLa cells were infected with HRV16 at an M.O.I of 3. At 3 h.p.i., ActoD (5 µg/mL) was added to selected samples. Caspase 8 was inhibited as indicated through addition caspase 8 inhibitor (4 µM) at 4 h.p.i. Cultures were frozen at 24 h.p.i. Once thawed, virus was clarified and titrated. Results are expressed as mean of 3 independent experiments. Error bars are +/− standard error of the mean. *p ≤ 0.05, ns – nonsignificant. ( b ) HRV16 restricts apoptotic pathways. O-HeLa cells were infected and treated with ActoD as in a) above. Cells were collected at 12 h.p.i and the effect of treatment and/or infection analysed by flow cytometry after staining with annexin V-FITC (An) and propidium iodide (Pi). Representative dual-parameter fluorescence density blots were acquired. ( c ) Bars are representative of percentage of viable (An−/Pi−), early apoptotic (An+/Pi−), late apoptotic (An+/Pi+) or necrotic (An−/Pi+) cells of total number of cells within each sample.

    Article Snippet: Cells were collected and resuspended in Annexin V binding buffer (HEPES; 10 mM, NaCl; 140 mM, CaCl2 ; 2.5 mM) and stained with Annexin V-FITC conjugate (ThermoFisher: ) and Propidium Iodide (Pi) (BD: 556463) before flow cytometric analysis as per manufacturer’s instructions.

    Techniques: Inhibition, Infection, Flow Cytometry, Cytometry, Staining, Fluorescence

    Characterization of pSer335 XErp1 antibody CSF extract was treated with Myc‐XErp1 IVT carrying the indicated combinations of the mutations DSG − (S33N S38N), DSA − (S284N S288N), ZBR − (C583A) and CaMKII − (T195A). Calcium was added, samples were taken at the indicated time points and as indicated treated with λ‐phosphatase. Samples were immunoblotted for the Myc‐tag and cyclin B2. The cyclin B2 membrane was stripped and reprobed for α‐tubulin. Asterisk indicates unspecific bands. Several lanes were removed at the dashed line. CSF extract was treated with Myc‐XErp1 CaMKII − ZBR − (T195A C583A) IVT at the indicated dilutions. An empty IVT not expressing XErp1 and an untreated condition were used as controls. Samples were taken, treated as indicated with λ‐phosphatase and immunoblotted for XErp1, pSer335 XErp1, and α‐tubulin. The XErp1 membrane was stripped and reprobed for the Myc‐tag. Asterisks indicate unspecific bands. CSF extract was treated with Myc‐XErp1 CaMKII − ZBR − (T195A C583A) IVT that was either wild‐type or mutated to alanine at Ser335. An empty IVT reaction not expressing Myc‐XErp1 served as control. Samples were taken, treated as indicated with λ‐phosphatase and immunoblotted for XErp1, the Myc‐tag, pSer335 XErp1, and α‐tubulin. Asterisks indicate unspecific bands. Source data are available online for this figure.

    Journal: EMBO Reports

    Article Title: Calcineurin promotes APC/C activation at meiotic exit by acting on both XErp1 and Cdc20

    doi: 10.15252/embr.201846433

    Figure Lengend Snippet: Characterization of pSer335 XErp1 antibody CSF extract was treated with Myc‐XErp1 IVT carrying the indicated combinations of the mutations DSG − (S33N S38N), DSA − (S284N S288N), ZBR − (C583A) and CaMKII − (T195A). Calcium was added, samples were taken at the indicated time points and as indicated treated with λ‐phosphatase. Samples were immunoblotted for the Myc‐tag and cyclin B2. The cyclin B2 membrane was stripped and reprobed for α‐tubulin. Asterisk indicates unspecific bands. Several lanes were removed at the dashed line. CSF extract was treated with Myc‐XErp1 CaMKII − ZBR − (T195A C583A) IVT at the indicated dilutions. An empty IVT not expressing XErp1 and an untreated condition were used as controls. Samples were taken, treated as indicated with λ‐phosphatase and immunoblotted for XErp1, pSer335 XErp1, and α‐tubulin. The XErp1 membrane was stripped and reprobed for the Myc‐tag. Asterisks indicate unspecific bands. CSF extract was treated with Myc‐XErp1 CaMKII − ZBR − (T195A C583A) IVT that was either wild‐type or mutated to alanine at Ser335. An empty IVT reaction not expressing Myc‐XErp1 served as control. Samples were taken, treated as indicated with λ‐phosphatase and immunoblotted for XErp1, the Myc‐tag, pSer335 XErp1, and α‐tubulin. Asterisks indicate unspecific bands. Source data are available online for this figure.

    Article Snippet: XErp1: 2 μl Myc‐XErp1 IVT were diluted in 8 μl CSF‐XB Buffer (10 mM HEPES; 100 mM KCl; 2 mM MgCl2 ; 0.1 mM CaCl2 ; 50 mM sucrose; 5 mM EGTA; pH = 7.7) and added to 0.5 μg α‐Myc antibodies coupled to Dynabeads® Protein G (Thermo Fisher).

    Techniques: Expressing

    Autophagy-deficiency during myoblast differentiation augments apoptotic signaling, ER-stress responses, and cell death. Quantitative analysis of early apoptotic (ANXA5 + PI − ) cells ( a ), late apoptotic (ANXA5 + PI + ) cells ( b ), and CASP3 activity ( c ) in SCR and sh Atg7 cells during differentiation. Representative immunoblots ( d ) and quantitative analysis ( e, f, g, h ) of BAX, BCL2, and HSPA (as well as calculated BAX:BCL2 ratio) in SCR and sh Atg7 cells during differentiation. Quantitative analysis of CAPN activity ( i ) in SCR and sh Atg7 cells during differentiation. *p

    Journal: Autophagy

    Article Title: Mitophagy regulates mitochondrial network signaling, oxidative stress, and apoptosis during myoblast differentiation

    doi: 10.1080/15548627.2019.1591672

    Figure Lengend Snippet: Autophagy-deficiency during myoblast differentiation augments apoptotic signaling, ER-stress responses, and cell death. Quantitative analysis of early apoptotic (ANXA5 + PI − ) cells ( a ), late apoptotic (ANXA5 + PI + ) cells ( b ), and CASP3 activity ( c ) in SCR and sh Atg7 cells during differentiation. Representative immunoblots ( d ) and quantitative analysis ( e, f, g, h ) of BAX, BCL2, and HSPA (as well as calculated BAX:BCL2 ratio) in SCR and sh Atg7 cells during differentiation. Quantitative analysis of CAPN activity ( i ) in SCR and sh Atg7 cells during differentiation. *p

    Article Snippet: Isolated cells were washed in PBS, resuspended in ANXA5 binding buffer (10 mM HEPES/NaOH, 150 mM NaCl, 1.8 mM CaCl2, pH 7.4) and incubated with 5 µL of ANXA5-FITC (ThermoFisher Scientific; A13199) and 10 µL of 50 µg/mL of PI (Sigma-Aldrich, P4170).

    Techniques: Activity Assay, Western Blot