cacl2 6h2 o  (Millipore)


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    Structured Review

    Millipore cacl2 6h2 o
    Cacl2 6h2 O, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacl2 6h2 o/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    cacl2 6h2 o - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Selection:

    Article Title: Engineering of a high lipid producing Yarrowia lipolytica strain
    Article Snippet: For routine maintenance and genetic transformation, strains were cultured in YPD (10 g/L yeast extract, 20 g/L bacto peptone, 20 g/L glucose) with the addition of 20 g/L agar for solid media and 300 μg/mL hygromycin B (Corning), 500 μg/mL nourseothricin (Werner Bioreagents) or 1 mg/mL zeocin (Invitrogen) for antibiotic selection. .. Initial concentrations of medium for 1 L batch and fed-batch bioreactors was: glucose T0 (150 g/L), sensient amberex 1003 yeast extract (3 g/L), sensient amberferm 4500 corn peptone (0.1 g/L), KH2 PO4 (4 g/L), MgSO4 ·7H2 O (2 g/L), CaCl2 ·6H2 O (0.8 g/L), NaCl (0.4 g/L), thiamine (12 mg/L), biotin (1 mg/L), trace elements [Na2 MoO4 ·2H2 O (160 mg/L), CuSO4 ·5H2 O (0.2 mg/L), H3 BO3 (40 mg/L), MnSO4 ·H2 O (180 mg/L), FeCl2 ·6H2 O (75 mg/L), ZnSO4 ·7H2 O (20 mg/L)], and Antifoam 204 (Sigma-Aldrich) (1 mL/L).

    In Vitro:

    Article Title: In Vitro Fermentation Patterns of Rice Bran Components by Human Gut Microbiota
    Article Snippet: Paragraph title: 2.3. In Vitro Fermentation ... In short, 50 mg each of FAXO, RBPP, their combination (MIX-FAXO with RBPP), and FOS used as control were mixed in 10 mL of sterile fermentation medium consisting of (per liter) peptone (2 g; Fisher Scientific), yeast extract (2 g; Alfa Aesar, Ward Hill, MA, USA), bile salts (0.5 g; Oxoid, Hampshire, UK), NaHCO3 (2 g), NaCl (0.1 g), K2 HPO4 (0.08 g), MgSO4 ·7H2 O (0.01 g), CaCl2 ·6H2 O (0.01 g), l -cysteine hydrochloride (0.5 g; Sigma-Aldrich, St. Louis, MO, USA), bovine hemin (50 mg; Sigma-Aldrich), Tween 80 (2 mL), vitamin K (10 μL; Sigma-Aldrich), and 0.025% (wt/vol) resazurin solution (4 mL).

    Article Title: Some putative prebiotics increase the severity of Salmonella enterica serovar Typhimurium infection in mice
    Article Snippet: .. In vitro fermentation of non-digestible dietary carbohydrates The fermentation study was performed using a basal medium containing: peptone water (2 g/L, Oxoid), yeast extract (2 g/L, Oxoid), NaCl (0.1 g/L, Merck), K2 HPO4 (0.04 g/L, Merck), KH2 PO4 (0.04 g/L, Merck), MgSO4 ·7H2 O (0.01 g/L, Merck), CaCl2 ·6H2 O (0.01 g/L, Sigma-Aldrich), NaHCO3 (2 g/L, Merck), haemin (0.005 g/L, Sigma-Aldrich), L-cystein HCL (0.5 g/L, Sigma-Aldrich), bile salts (0.5 g/L, Oxoid), Tween 80 (2 ml/L, Merck), vitamin K1 (10 μl/L, Sigma-Aldrich), resazurin (0.001 g/L, Sigma-Aldrich) and 1% (wt/vol) test carbohydrate (inulin, FOS, XOS, GOS, beta-glucan, apple pectin, polydextrose and glucose) [ ]. ..

    Article Title: Comparative prebiotic activity of mixtures of cereal grain polysaccharides
    Article Snippet: Paragraph title: In -vitro fermentation ... The medium contained per litre: 2 g of peptone water (Oxoid Ltd., Basingstoke, United Kingdom), 2 g of yeast extract (Oxoid), 0.1 g of NaCl, 0.04 g of K2 HPO4 , 0.01 g of MgSO4 ·7H2 O, 0.01 g of CaCl2 ·6H2 O, 2 g of NaHCO3 , 0.005 g of hemein (Sigma), 0.5 g of l -cysteine HCl (Sigma), 0.5 g of bile salts (Oxoid), 2 mL of Tween 80, 10 µL of vitamin K (Sigma).

    Fluorescence:

    Article Title: Engineering of a high lipid producing Yarrowia lipolytica strain
    Article Snippet: Medium used for fluorescence assay in 96-well or 48-well plates, and shake flasks was: urea (0.5 g/L), yeast extract (1.5 g/L), casamino acids (0.85 g/L), YNB without amino acids and ammonium sulfate (1.7 g/L), glucose (100 g/L), potassium hydrogen phthalate buffer adjusted to pH 5.5 (5.1 g/L). .. Initial concentrations of medium for 1 L batch and fed-batch bioreactors was: glucose T0 (150 g/L), sensient amberex 1003 yeast extract (3 g/L), sensient amberferm 4500 corn peptone (0.1 g/L), KH2 PO4 (4 g/L), MgSO4 ·7H2 O (2 g/L), CaCl2 ·6H2 O (0.8 g/L), NaCl (0.4 g/L), thiamine (12 mg/L), biotin (1 mg/L), trace elements [Na2 MoO4 ·2H2 O (160 mg/L), CuSO4 ·5H2 O (0.2 mg/L), H3 BO3 (40 mg/L), MnSO4 ·H2 O (180 mg/L), FeCl2 ·6H2 O (75 mg/L), ZnSO4 ·7H2 O (20 mg/L)], and Antifoam 204 (Sigma-Aldrich) (1 mL/L).

    Article Title: Engineered Coatings for Titanium Implants To Present Ultralow Doses of BMP-7
    Article Snippet: Afterward, 100 μL was transferred from each well into a black 96-well plate (Greiner Bio-One), and fluorescence was measured immediately at 590 nm (with excitation at 560 nm) (Infinite M200 PRO plate reader, Tecan Group Ltd.). .. A standard calibration curve was established to detect dilutions of 0−1600 μg/mL CaCl2 ·6H2 O (Sigma-Aldrich).

    Isolation:

    Article Title: The chimeric gene CHRFAM7A, a partial duplication of the CHRNA7 gene, is a dominant negative regulator of α7*nAChR function
    Article Snippet: Oocytes were isolated by mechanical and enzymatic action using type I collagenase (Sigma, Basel Switzerland) at 0.2% in a medium deprived of calcium. .. Stage V and VI oocytes were placed in 96 wells microtiter plates (NUNC) in BARTH medium that contained 88 mM NaCl, 1 mM KCl, 2.4 mM NaHCO3 , 10 mM HEPES, 0.82 mM MgSO4 ·7H2 O, 0.33 mM Ca(NO3 )2 ·4H2 O, 0.41 mM CaCl2 ·6H2 O, adjusted to pH 7.4 with NaOH and supplemented with kanamycin (20 mg/ml), penicillin (100 U/ml) and streptomycin (100 U/ml) (Sigma).

    Dissection:

    Article Title: Isolation and culture of endothelial cells, pericytes and perivascular resident macrophage-like melanocytes from the young mouse ear
    Article Snippet: Paragraph title: Surgery and dissection reagents ... Ketamine hydrochloride, injectable, 100 mg ml−1 (JHP Pharmaceuticals, cat. no. 074666) Xylazine, sterile solution, 100 mg ml−1 (Lloyd, cat. no. LPFL04821) Euthanasia solution, 100 ml (Virbac Animal Health, cat. no. 710101) Ethanol, 70% (vol/vol; Sigma, cat. no. 459844) Sodium chloride, 500 g (NaCl; Fisher Scientific, cat. no. S271-500) Potassium chloride, 500 g (KCl; Fisher Scientific, cat. no. P217-500) Calcium chloride hexahydrate, 1 kg (CaCl2 ·6H2 O; Sigma, cat. no. 442909) Magnesium chloride hexahydrate, 500 g (MgCl2 ·6H2 O; Fisher Scientific, cat. no. M33-500) Sodium phosphate monobasic, 500 g (NaH2 PO4·H2 O; Fisher Scientific, cat. no. S369-500) HEPES, 500 g (Fisher Scientific, cat. no. BP310-500) d -(+)-Glucose, 1 kg (Sigma, cat. no. G7528) Lidocaine HCl

    Cell Culture:

    Article Title: Engineering of a high lipid producing Yarrowia lipolytica strain
    Article Snippet: All Yarrowia strains were cultured at 30 °C. .. Initial concentrations of medium for 1 L batch and fed-batch bioreactors was: glucose T0 (150 g/L), sensient amberex 1003 yeast extract (3 g/L), sensient amberferm 4500 corn peptone (0.1 g/L), KH2 PO4 (4 g/L), MgSO4 ·7H2 O (2 g/L), CaCl2 ·6H2 O (0.8 g/L), NaCl (0.4 g/L), thiamine (12 mg/L), biotin (1 mg/L), trace elements [Na2 MoO4 ·2H2 O (160 mg/L), CuSO4 ·5H2 O (0.2 mg/L), H3 BO3 (40 mg/L), MnSO4 ·H2 O (180 mg/L), FeCl2 ·6H2 O (75 mg/L), ZnSO4 ·7H2 O (20 mg/L)], and Antifoam 204 (Sigma-Aldrich) (1 mL/L).

    Mouse Assay:

    Article Title: Isolation and culture of endothelial cells, pericytes and perivascular resident macrophage-like melanocytes from the young mouse ear
    Article Snippet: Mice aged 10–15 d (C57BL/6J; either sex can be used) CAUTION Experiments involving live rodents must conform to all relevant governmental and institutional regulations. .. Ketamine hydrochloride, injectable, 100 mg ml−1 (JHP Pharmaceuticals, cat. no. 074666) Xylazine, sterile solution, 100 mg ml−1 (Lloyd, cat. no. LPFL04821) Euthanasia solution, 100 ml (Virbac Animal Health, cat. no. 710101) Ethanol, 70% (vol/vol; Sigma, cat. no. 459844) Sodium chloride, 500 g (NaCl; Fisher Scientific, cat. no. S271-500) Potassium chloride, 500 g (KCl; Fisher Scientific, cat. no. P217-500) Calcium chloride hexahydrate, 1 kg (CaCl2 ·6H2 O; Sigma, cat. no. 442909) Magnesium chloride hexahydrate, 500 g (MgCl2 ·6H2 O; Fisher Scientific, cat. no. M33-500) Sodium phosphate monobasic, 500 g (NaH2 PO4·H2 O; Fisher Scientific, cat. no. S369-500) HEPES, 500 g (Fisher Scientific, cat. no. BP310-500) d -(+)-Glucose, 1 kg (Sigma, cat. no. G7528) Lidocaine HCl

    Produced:

    Article Title: Engineered Coatings for Titanium Implants To Present Ultralow Doses of BMP-7
    Article Snippet: Matrix mineralization was determined by a direct assay of total calcium produced by the seeded cells under the same conditions as for the proliferation assay. .. A standard calibration curve was established to detect dilutions of 0−1600 μg/mL CaCl2 ·6H2 O (Sigma-Aldrich).

    Concentration Assay:

    Article Title: Sol–Gel Synthesis of Silica-Based Materials with Different Percentages of PEG or PCL and High Chlorogenic Acid Content
    Article Snippet: The SBF solution contained an ion concentration almost equal to those of human blood plasma ( ). .. The SBF was prepared by dissolving reagent grade chemicals NaCl, NaHCO3 , KCl, MgCl2 , 1 M HCl, CaCl2 ·6H2 O, and Na2 SO4 (Sigma-Aldrich, St. Louis, MO, USA).

    Incubation:

    Article Title: Engineered Coatings for Titanium Implants To Present Ultralow Doses of BMP-7
    Article Snippet: Prior the assay, samples were transferred into a new 24-well plate and a fresh low serum nonosteogenic medium was added to all wells. alamarBlue reagent was then added at 50 μL/well followed by incubation for exactly 2.5 h at 37 °C. .. A standard calibration curve was established to detect dilutions of 0−1600 μg/mL CaCl2 ·6H2 O (Sigma-Aldrich).

    Article Title: Properties of carbon nanotube-dispersed Sr-hydroxyapatite injectable material for bone defects
    Article Snippet: The solution was prepared by dissolving each component NaCl, NaHCO3 , KCl, MgCl2 , HCl 1M, CaCl2 ·6H2 O and Na2 SO4 (ACS reagent grade, Sigma-Aldrich) in distilled water at 37°C and buffered at pH 7.4 using tris(hydroxymethyl)-aminoethane (Sigma-Aldrich, Italy). .. After 7days of incubation time, the 15SrHA/CNT gel material was washed with distilled water (3 times) and dried in the oven at 40°C overnight.

    Proliferation Assay:

    Article Title: Engineered Coatings for Titanium Implants To Present Ultralow Doses of BMP-7
    Article Snippet: Matrix mineralization was determined by a direct assay of total calcium produced by the seeded cells under the same conditions as for the proliferation assay. .. A standard calibration curve was established to detect dilutions of 0−1600 μg/mL CaCl2 ·6H2 O (Sigma-Aldrich).

    Expressing:

    Article Title: The chimeric gene CHRFAM7A, a partial duplication of the CHRNA7 gene, is a dominant negative regulator of α7*nAChR function
    Article Snippet: Paragraph title: 2.3 Oocyte expression ... Stage V and VI oocytes were placed in 96 wells microtiter plates (NUNC) in BARTH medium that contained 88 mM NaCl, 1 mM KCl, 2.4 mM NaHCO3 , 10 mM HEPES, 0.82 mM MgSO4 ·7H2 O, 0.33 mM Ca(NO3 )2 ·4H2 O, 0.41 mM CaCl2 ·6H2 O, adjusted to pH 7.4 with NaOH and supplemented with kanamycin (20 mg/ml), penicillin (100 U/ml) and streptomycin (100 U/ml) (Sigma).

    Injection:

    Article Title: The chimeric gene CHRFAM7A, a partial duplication of the CHRNA7 gene, is a dominant negative regulator of α7*nAChR function
    Article Snippet: Stage V and VI oocytes were placed in 96 wells microtiter plates (NUNC) in BARTH medium that contained 88 mM NaCl, 1 mM KCl, 2.4 mM NaHCO3 , 10 mM HEPES, 0.82 mM MgSO4 ·7H2 O, 0.33 mM Ca(NO3 )2 ·4H2 O, 0.41 mM CaCl2 ·6H2 O, adjusted to pH 7.4 with NaOH and supplemented with kanamycin (20 mg/ml), penicillin (100 U/ml) and streptomycin (100 U/ml) (Sigma). .. Nuclear injections of 2 ng of cDNA expression vectors, containing equal molar ratios of the genes of interest were performed with an automatic injection system [ ].

    Transformation Assay:

    Article Title: Engineering of a high lipid producing Yarrowia lipolytica strain
    Article Snippet: For routine maintenance and genetic transformation, strains were cultured in YPD (10 g/L yeast extract, 20 g/L bacto peptone, 20 g/L glucose) with the addition of 20 g/L agar for solid media and 300 μg/mL hygromycin B (Corning), 500 μg/mL nourseothricin (Werner Bioreagents) or 1 mg/mL zeocin (Invitrogen) for antibiotic selection. .. Initial concentrations of medium for 1 L batch and fed-batch bioreactors was: glucose T0 (150 g/L), sensient amberex 1003 yeast extract (3 g/L), sensient amberferm 4500 corn peptone (0.1 g/L), KH2 PO4 (4 g/L), MgSO4 ·7H2 O (2 g/L), CaCl2 ·6H2 O (0.8 g/L), NaCl (0.4 g/L), thiamine (12 mg/L), biotin (1 mg/L), trace elements [Na2 MoO4 ·2H2 O (160 mg/L), CuSO4 ·5H2 O (0.2 mg/L), H3 BO3 (40 mg/L), MnSO4 ·H2 O (180 mg/L), FeCl2 ·6H2 O (75 mg/L), ZnSO4 ·7H2 O (20 mg/L)], and Antifoam 204 (Sigma-Aldrich) (1 mL/L).

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    Millipore dulbecco s phosphate buffered saline
    Dulbecco S Phosphate Buffered Saline, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbecco s phosphate buffered saline/product/Millipore
    Average 95 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    dulbecco s phosphate buffered saline - by Bioz Stars, 2020-02
    95/100 stars
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    79
    Millipore cytokine homogenate buffer
    Leptin and <t>cytokine</t> levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.
    Cytokine Homogenate Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokine homogenate buffer/product/Millipore
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cytokine homogenate buffer - by Bioz Stars, 2020-02
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    84
    Millipore homogenate buffer
    Leptin and <t>cytokine</t> levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.
    Homogenate Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/homogenate buffer/product/Millipore
    Average 84 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    homogenate buffer - by Bioz Stars, 2020-02
    84/100 stars
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    Leptin and cytokine levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.

    Journal: BioMed Research International

    Article Title: Advanced Glycation End Products Induce Obesity and Hepatosteatosis in CD-1 Wild-Type Mice

    doi: 10.1155/2016/7867852

    Figure Lengend Snippet: Leptin and cytokine levels (IL-6 and TNF- α ) after 4 and 6 weeks on the diets. Consistent with the weight gain and epididymal fat pad weights, leptin levels were significantly higher after 4 and 6 weeks in the HAGE-HF group compared to the other groups. Also consistent with the liver histology and presence of inflammation after 6 weeks on the diet, IL-6 and TNF- α levels were significantly higher in the HAGE-HF group after 6 weeks on the diet.

    Article Snippet: The remaining liver was washed with cold HBSS and suspended in cytokine homogenate buffer (150 mM NaCl, 15 mM Tris, 1 mM CaCl2 ·2H2 O, and 1 mM MgCl2 ·6H2 O, adjusted to pH 7.4) plus 100x protease inhibitor cocktail 1 (Calbiochem, La Jolla, California) to a total volume of 10 mL and homogenized on ice using a Polytron® Homogenizer (Kinematica Inc., Bohemia, NY).

    Techniques: