ca2 ionophore a23187  (Millipore)


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    Name:
    Calcium Ionophore A23187
    Description:

    Catalog Number:
    c7522
    Price:
    None
    Applications:
    Ionophore highly selective for Ca2+. Potentiates responses to NMDA but not quisqualate. In cell culture, stimulates nitric oxide production by calmodulin-dependent constitutive nitric oxide synthase.
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    Structured Review

    Millipore ca2 ionophore a23187
    Calcium Ionophore A23187

    https://www.bioz.com/result/ca2 ionophore a23187/product/Millipore
    Average 99 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    ca2 ionophore a23187 - by Bioz Stars, 2020-11
    99/100 stars

    Images

    1) Product Images from "Plasticity between MyoC- and MyoA-Glideosomes: An Example of Functional Compensation in Toxoplasma gondii Invasion"

    Article Title: Plasticity between MyoC- and MyoA-Glideosomes: An Example of Functional Compensation in Toxoplasma gondii Invasion

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1004504

    MyoC-glideosome is not involved in cell division but in invasion. A. Intracellular growth assay performed on GAP45-iKO and GAP45-iKO/GAP80-KO strains by determining the number of parasites per vacuole after 48 hours ± ATc. Data are represented as mean ± SD. B. Gliding assay performed on poly-L-lysine coated coverslips with GAP45-iKO and GAP45-iKO/GAP80-KO strains after 42 hours ± ATc. C. Ionophore-induced egress assay of GAP45-iKO and GAP45-iKO/GAP80-KO strains was performed by treating the parasites with DMSO or Ca2+-ionophore A23187 for 5 min after 56 hours ± ATc before The results are expressed as a percentage of ruptured vacuoles and represented as mean ± SD. D. Invasiveness of GAP45-iKO and GAP45-iKO/GAP80-KO strains was determined using a two-color immunofluorescence assay performed after 42 hours ± ATc. Intracellular: invaded parasites, extracellular: attached parasites. Data are represented as mean ± SD. The significance of the data was evaluated using a parametric paired t-test and the two-tailed p-value is written on the graph. E. Co-IP performed on metabolically labeled wild type and MyoA-KO parasites using anti-MLC1 antibodies. F. In MyoA-KO, MycMyoC-iKO relocalized to the periphery of the parasites up to the apical basal ring in addition to its basal localization. Two exposures are presented for MycMyoC localization. Scale bars: 2 µm. G. Western-blot of total extract of MycMyoC-iKO and MycMyoC-iKO/MyoA-iKO analyzed using anti-MyoA, anti-MLC1 and anti-Myc antibodies. The loading control was done at the same time with anti-PRF and fluorescent secondary antibodies on the same membrane as MLC1 for the upper panel and as Myc for the lower panel. H. Ionophore-induced egress assay of MyoC-iKO and MyoC-iKO/MyoA-KO strains performed by treating the parasites with DMSO or Ca2+-ionophore A23187 for 5 min after 54 hours ± ATc before The results are expressed as a percentage of ruptured vacuoles and represented as mean ± SD. I. Red/green invasion assay performed after 42 hours ± ATc. Intracellular: invaded parasites, extracellular: attached parasites. Data are represented as mean ± SD. The significance of the data was evaluated using a parametric paired t-test and the two-tailed p-value is written on the graph.
    Figure Legend Snippet: MyoC-glideosome is not involved in cell division but in invasion. A. Intracellular growth assay performed on GAP45-iKO and GAP45-iKO/GAP80-KO strains by determining the number of parasites per vacuole after 48 hours ± ATc. Data are represented as mean ± SD. B. Gliding assay performed on poly-L-lysine coated coverslips with GAP45-iKO and GAP45-iKO/GAP80-KO strains after 42 hours ± ATc. C. Ionophore-induced egress assay of GAP45-iKO and GAP45-iKO/GAP80-KO strains was performed by treating the parasites with DMSO or Ca2+-ionophore A23187 for 5 min after 56 hours ± ATc before The results are expressed as a percentage of ruptured vacuoles and represented as mean ± SD. D. Invasiveness of GAP45-iKO and GAP45-iKO/GAP80-KO strains was determined using a two-color immunofluorescence assay performed after 42 hours ± ATc. Intracellular: invaded parasites, extracellular: attached parasites. Data are represented as mean ± SD. The significance of the data was evaluated using a parametric paired t-test and the two-tailed p-value is written on the graph. E. Co-IP performed on metabolically labeled wild type and MyoA-KO parasites using anti-MLC1 antibodies. F. In MyoA-KO, MycMyoC-iKO relocalized to the periphery of the parasites up to the apical basal ring in addition to its basal localization. Two exposures are presented for MycMyoC localization. Scale bars: 2 µm. G. Western-blot of total extract of MycMyoC-iKO and MycMyoC-iKO/MyoA-iKO analyzed using anti-MyoA, anti-MLC1 and anti-Myc antibodies. The loading control was done at the same time with anti-PRF and fluorescent secondary antibodies on the same membrane as MLC1 for the upper panel and as Myc for the lower panel. H. Ionophore-induced egress assay of MyoC-iKO and MyoC-iKO/MyoA-KO strains performed by treating the parasites with DMSO or Ca2+-ionophore A23187 for 5 min after 54 hours ± ATc before The results are expressed as a percentage of ruptured vacuoles and represented as mean ± SD. I. Red/green invasion assay performed after 42 hours ± ATc. Intracellular: invaded parasites, extracellular: attached parasites. Data are represented as mean ± SD. The significance of the data was evaluated using a parametric paired t-test and the two-tailed p-value is written on the graph.

    Techniques Used: Growth Assay, Gliding Assay, Immunofluorescence, Two Tailed Test, Co-Immunoprecipitation Assay, Metabolic Labelling, Labeling, Western Blot, Invasion Assay

    Related Articles

    Positive Control:

    Article Title: Peptidoglycan Induces Mobilization of the Surface Marker for Activation Marker CD66b in Human Neutrophils but Not in Eosinophils
    Article Snippet: .. As a positive control for the mobilization of surface markers, neutrophils were incubated with N -formyl-Met-Leu-Phe (fMLP) (1 μM; Sigma) and eosinophils were incubated with the calcium ionophore A23187 (1 μM; Sigma). .. Thereafter, the cells were incubated with fluorescein isothiocyanate (FITC)-conjugated antibodies (CD11b, CD44, CD63, CD66b, or CD69 [DAKOPATTS, Glostrup, Denmark] or an isotype-matched FITC-conjugated irrelevant monoclonal antibody at the same concentration [Immunotech, Marseille, France]).

    Fluorescence:

    Article Title: Myeloid-Specific Deletion of Peptidylarginine Deiminase 4 Mitigates Atherosclerosis
    Article Snippet: .. Detection of NETs by Immunofluorescence Microscopy Fluorescence-activated cell sorting-sorted peritoneal neutrophils (1 × 106 cells/ml) were pre-incubated in poly-l -lysine coated cover slips for 15 min, supernatants were removed, and indicated stimuli were added [calcium ionophore, A23187 (10 µM), EMD Millipore, Billerica, MA, USA] for 4 h at 37°C. .. Cells were stained with rabbit anti-citrullinated histone 3 (Abcam, ab 5103) for 1 h, followed by staining with Alexa Fluor 488-conjugated donkey anti-rat IgG and Alexa Fluor 555-conjugated donkey anti-rabbit IgG (Life Technologies).

    Microscopy:

    Article Title: Myeloid-Specific Deletion of Peptidylarginine Deiminase 4 Mitigates Atherosclerosis
    Article Snippet: .. Detection of NETs by Immunofluorescence Microscopy Fluorescence-activated cell sorting-sorted peritoneal neutrophils (1 × 106 cells/ml) were pre-incubated in poly-l -lysine coated cover slips for 15 min, supernatants were removed, and indicated stimuli were added [calcium ionophore, A23187 (10 µM), EMD Millipore, Billerica, MA, USA] for 4 h at 37°C. .. Cells were stained with rabbit anti-citrullinated histone 3 (Abcam, ab 5103) for 1 h, followed by staining with Alexa Fluor 488-conjugated donkey anti-rat IgG and Alexa Fluor 555-conjugated donkey anti-rabbit IgG (Life Technologies).

    Concentration Assay:

    Article Title: Labeling and exocytosis of secretory compartments in RBL mastocytes by polystyrene and mesoporous silica nanoparticles
    Article Snippet: .. The calcium ionophore A23187 and the calcium-chelating ligand EGTA (both from Sigma Aldrich) were used at the concentration of 1 μM and 5 mM, respectively. .. Methyl-β-cyclodextrin (MbCD) (cod C4805; Sigma-Aldrich) was used at 5 mM final concentration.

    Incubation:

    Article Title: Peptidoglycan Induces Mobilization of the Surface Marker for Activation Marker CD66b in Human Neutrophils but Not in Eosinophils
    Article Snippet: .. As a positive control for the mobilization of surface markers, neutrophils were incubated with N -formyl-Met-Leu-Phe (fMLP) (1 μM; Sigma) and eosinophils were incubated with the calcium ionophore A23187 (1 μM; Sigma). .. Thereafter, the cells were incubated with fluorescein isothiocyanate (FITC)-conjugated antibodies (CD11b, CD44, CD63, CD66b, or CD69 [DAKOPATTS, Glostrup, Denmark] or an isotype-matched FITC-conjugated irrelevant monoclonal antibody at the same concentration [Immunotech, Marseille, France]).

    Article Title: Functional Characterization of Dense Granule Proteins in Toxoplasma gondii RH Strain Using CRISPR-Cas9 System
    Article Snippet: .. After 30–36 h of incubation, the wells were washed twice with sterile PBS and 3 μM calcium ionophore A23187 (Sigma) diluted in DMSO were added to the HFF cells. .. Live cell microscopy was used to monitor the timing of parasite egress from HFF cells infected with the WT strain compared with HFF cells infected with the mutant strains after addition of 3 μM calcium ionophore A23187.

    other:

    Article Title: Mitochondrial and calcium perturbations in rat CNS neurons induce calpain-cleavage of Parkin: Phosphatase inhibition stabilizes pSer65Parkin reducing its calpain-cleavage
    Article Snippet: Therefore, we established for the first time to our knowledge, that Parkin is cleaved by calpain upon treatment with the ionophore A23187, which disrupts calcium homeostasis.

    Immunofluorescence:

    Article Title: Myeloid-Specific Deletion of Peptidylarginine Deiminase 4 Mitigates Atherosclerosis
    Article Snippet: .. Detection of NETs by Immunofluorescence Microscopy Fluorescence-activated cell sorting-sorted peritoneal neutrophils (1 × 106 cells/ml) were pre-incubated in poly-l -lysine coated cover slips for 15 min, supernatants were removed, and indicated stimuli were added [calcium ionophore, A23187 (10 µM), EMD Millipore, Billerica, MA, USA] for 4 h at 37°C. .. Cells were stained with rabbit anti-citrullinated histone 3 (Abcam, ab 5103) for 1 h, followed by staining with Alexa Fluor 488-conjugated donkey anti-rat IgG and Alexa Fluor 555-conjugated donkey anti-rabbit IgG (Life Technologies).

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  • 99
    Millipore ca2 ionophore a23187
    MyoC-glideosome is not involved in cell division but in invasion. A. Intracellular growth assay performed on GAP45-iKO and GAP45-iKO/GAP80-KO strains by determining the number of parasites per vacuole after 48 hours ± ATc. Data are represented as mean ± SD. B. Gliding assay performed on poly-L-lysine coated coverslips with GAP45-iKO and GAP45-iKO/GAP80-KO strains after 42 hours ± ATc. C. Ionophore-induced egress assay of GAP45-iKO and GAP45-iKO/GAP80-KO strains was performed by treating the parasites with DMSO or <t>Ca2+-ionophore</t> <t>A23187</t> for 5 min after 56 hours ± ATc before The results are expressed as a percentage of ruptured vacuoles and represented as mean ± SD. D. Invasiveness of GAP45-iKO and GAP45-iKO/GAP80-KO strains was determined using a two-color immunofluorescence assay performed after 42 hours ± ATc. Intracellular: invaded parasites, extracellular: attached parasites. Data are represented as mean ± SD. The significance of the data was evaluated using a parametric paired t-test and the two-tailed p-value is written on the graph. E. Co-IP performed on metabolically labeled wild type and MyoA-KO parasites using anti-MLC1 antibodies. F. In MyoA-KO, MycMyoC-iKO relocalized to the periphery of the parasites up to the apical basal ring in addition to its basal localization. Two exposures are presented for MycMyoC localization. Scale bars: 2 µm. G. Western-blot of total extract of MycMyoC-iKO and MycMyoC-iKO/MyoA-iKO analyzed using anti-MyoA, anti-MLC1 and anti-Myc antibodies. The loading control was done at the same time with anti-PRF and fluorescent secondary antibodies on the same membrane as MLC1 for the upper panel and as Myc for the lower panel. H. Ionophore-induced egress assay of MyoC-iKO and MyoC-iKO/MyoA-KO strains performed by treating the parasites with DMSO or Ca2+-ionophore A23187 for 5 min after 54 hours ± ATc before The results are expressed as a percentage of ruptured vacuoles and represented as mean ± SD. I. Red/green invasion assay performed after 42 hours ± ATc. Intracellular: invaded parasites, extracellular: attached parasites. Data are represented as mean ± SD. The significance of the data was evaluated using a parametric paired t-test and the two-tailed p-value is written on the graph.
    Ca2 Ionophore A23187, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ca2 ionophore a23187/product/Millipore
    Average 99 stars, based on 124 article reviews
    Price from $9.99 to $1999.99
    ca2 ionophore a23187 - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

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    MyoC-glideosome is not involved in cell division but in invasion. A. Intracellular growth assay performed on GAP45-iKO and GAP45-iKO/GAP80-KO strains by determining the number of parasites per vacuole after 48 hours ± ATc. Data are represented as mean ± SD. B. Gliding assay performed on poly-L-lysine coated coverslips with GAP45-iKO and GAP45-iKO/GAP80-KO strains after 42 hours ± ATc. C. Ionophore-induced egress assay of GAP45-iKO and GAP45-iKO/GAP80-KO strains was performed by treating the parasites with DMSO or Ca2+-ionophore A23187 for 5 min after 56 hours ± ATc before The results are expressed as a percentage of ruptured vacuoles and represented as mean ± SD. D. Invasiveness of GAP45-iKO and GAP45-iKO/GAP80-KO strains was determined using a two-color immunofluorescence assay performed after 42 hours ± ATc. Intracellular: invaded parasites, extracellular: attached parasites. Data are represented as mean ± SD. The significance of the data was evaluated using a parametric paired t-test and the two-tailed p-value is written on the graph. E. Co-IP performed on metabolically labeled wild type and MyoA-KO parasites using anti-MLC1 antibodies. F. In MyoA-KO, MycMyoC-iKO relocalized to the periphery of the parasites up to the apical basal ring in addition to its basal localization. Two exposures are presented for MycMyoC localization. Scale bars: 2 µm. G. Western-blot of total extract of MycMyoC-iKO and MycMyoC-iKO/MyoA-iKO analyzed using anti-MyoA, anti-MLC1 and anti-Myc antibodies. The loading control was done at the same time with anti-PRF and fluorescent secondary antibodies on the same membrane as MLC1 for the upper panel and as Myc for the lower panel. H. Ionophore-induced egress assay of MyoC-iKO and MyoC-iKO/MyoA-KO strains performed by treating the parasites with DMSO or Ca2+-ionophore A23187 for 5 min after 54 hours ± ATc before The results are expressed as a percentage of ruptured vacuoles and represented as mean ± SD. I. Red/green invasion assay performed after 42 hours ± ATc. Intracellular: invaded parasites, extracellular: attached parasites. Data are represented as mean ± SD. The significance of the data was evaluated using a parametric paired t-test and the two-tailed p-value is written on the graph.

    Journal: PLoS Pathogens

    Article Title: Plasticity between MyoC- and MyoA-Glideosomes: An Example of Functional Compensation in Toxoplasma gondii Invasion

    doi: 10.1371/journal.ppat.1004504

    Figure Lengend Snippet: MyoC-glideosome is not involved in cell division but in invasion. A. Intracellular growth assay performed on GAP45-iKO and GAP45-iKO/GAP80-KO strains by determining the number of parasites per vacuole after 48 hours ± ATc. Data are represented as mean ± SD. B. Gliding assay performed on poly-L-lysine coated coverslips with GAP45-iKO and GAP45-iKO/GAP80-KO strains after 42 hours ± ATc. C. Ionophore-induced egress assay of GAP45-iKO and GAP45-iKO/GAP80-KO strains was performed by treating the parasites with DMSO or Ca2+-ionophore A23187 for 5 min after 56 hours ± ATc before The results are expressed as a percentage of ruptured vacuoles and represented as mean ± SD. D. Invasiveness of GAP45-iKO and GAP45-iKO/GAP80-KO strains was determined using a two-color immunofluorescence assay performed after 42 hours ± ATc. Intracellular: invaded parasites, extracellular: attached parasites. Data are represented as mean ± SD. The significance of the data was evaluated using a parametric paired t-test and the two-tailed p-value is written on the graph. E. Co-IP performed on metabolically labeled wild type and MyoA-KO parasites using anti-MLC1 antibodies. F. In MyoA-KO, MycMyoC-iKO relocalized to the periphery of the parasites up to the apical basal ring in addition to its basal localization. Two exposures are presented for MycMyoC localization. Scale bars: 2 µm. G. Western-blot of total extract of MycMyoC-iKO and MycMyoC-iKO/MyoA-iKO analyzed using anti-MyoA, anti-MLC1 and anti-Myc antibodies. The loading control was done at the same time with anti-PRF and fluorescent secondary antibodies on the same membrane as MLC1 for the upper panel and as Myc for the lower panel. H. Ionophore-induced egress assay of MyoC-iKO and MyoC-iKO/MyoA-KO strains performed by treating the parasites with DMSO or Ca2+-ionophore A23187 for 5 min after 54 hours ± ATc before The results are expressed as a percentage of ruptured vacuoles and represented as mean ± SD. I. Red/green invasion assay performed after 42 hours ± ATc. Intracellular: invaded parasites, extracellular: attached parasites. Data are represented as mean ± SD. The significance of the data was evaluated using a parametric paired t-test and the two-tailed p-value is written on the graph.

    Article Snippet: Parasite-infected host cells were then incubated for 5 min at 37°C with DMEM containing 0.06% DMSO or 3 µM of the Ca2+ ionophore A23187 from Streptomyces chartreusensis (calbiochem) before fixation.

    Techniques: Growth Assay, Gliding Assay, Immunofluorescence, Two Tailed Test, Co-Immunoprecipitation Assay, Metabolic Labelling, Labeling, Western Blot, Invasion Assay