ca2 indicator fluo 3 am  (Thermo Fisher)


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    Name:
    Fluo 3 AM Calcium Indicator
    Description:
    Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2 Fluo 3 has been used to image the spatial dynamics of Ca2 signaling in flow cytometry experiments involving photoactivation of caged chelators second messengers and neurotransmitters and for cell based pharmacological screening Fluo 4 is an analog of fluo 3 with the two chlorine substituents replaced by fluorines which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells These indicators are useful for fluorescence and confocal microscopy flow cytometry and microplate screening applications Calcium Indicator AM Ester Specifications • Label Ex Em of Ca2 bound form Fluo 3 506 526 nm • Fluorescence intensity increase upon binding Ca2 100 fold• Kd for Ca2 in buffer 335 nM• Exhibit fluorescence increase upon binding Ca2 with little shift in wavelengthUsing TPEN to Control Heavy Metal Cations In addition BAPTA based indicators such as these bind various heavy metal cations e g Mn2 Zn2 Pb2 with substantially higher affinity than Ca2 Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal selective chelator TPEN More Choices for Fluorescent Calcium Indicators We offer a large selection of Molecular Probes calcium indicators for use in various experimental scenarios For more information review Fluorescent Ca2 Indicators Excited with Visible Light Section 19 3 in the Molecular Probes Handbook For UV excitable Ca2 indicators protein based Ca2 indicators conjugates of Ca2 indicators and for fluorescence based indicators of other metal ions i e Mg2 Zn2 review Indicators for Ca2 Mg2 Zn2 and Other Metal Ions Chapter 19 in the Molecular Probes Handbook For Research Use Only Not for human or animal therapeutic or diagnostic use
    Catalog Number:
    f1241
    Price:
    None
    Applications:
    Calcium Detection|Calcium Indicator Assays|Cell Analysis|Cell-Based Ion Channel Assays|Cell-Based Reporter Assays|Cell-Based Second Messenger Assays|Cellular Imaging|G-Protein Coupled Receptor Biology|Industrial & Applied Science|Ion Channel Biology|Ionic Homeostasis & Signaling|Pharma & Biopharma|Target & Lead Identification & Validation|Cell Viability, Proliferation & Function
    Category:
    Labeling Detection Products
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    Structured Review

    Thermo Fisher ca2 indicator fluo 3 am
    Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2 Fluo 3 has been used to image the spatial dynamics of Ca2 signaling in flow cytometry experiments involving photoactivation of caged chelators second messengers and neurotransmitters and for cell based pharmacological screening Fluo 4 is an analog of fluo 3 with the two chlorine substituents replaced by fluorines which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells These indicators are useful for fluorescence and confocal microscopy flow cytometry and microplate screening applications Calcium Indicator AM Ester Specifications • Label Ex Em of Ca2 bound form Fluo 3 506 526 nm • Fluorescence intensity increase upon binding Ca2 100 fold• Kd for Ca2 in buffer 335 nM• Exhibit fluorescence increase upon binding Ca2 with little shift in wavelengthUsing TPEN to Control Heavy Metal Cations In addition BAPTA based indicators such as these bind various heavy metal cations e g Mn2 Zn2 Pb2 with substantially higher affinity than Ca2 Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal selective chelator TPEN More Choices for Fluorescent Calcium Indicators We offer a large selection of Molecular Probes calcium indicators for use in various experimental scenarios For more information review Fluorescent Ca2 Indicators Excited with Visible Light Section 19 3 in the Molecular Probes Handbook For UV excitable Ca2 indicators protein based Ca2 indicators conjugates of Ca2 indicators and for fluorescence based indicators of other metal ions i e Mg2 Zn2 review Indicators for Ca2 Mg2 Zn2 and Other Metal Ions Chapter 19 in the Molecular Probes Handbook For Research Use Only Not for human or animal therapeutic or diagnostic use
    https://www.bioz.com/result/ca2 indicator fluo 3 am/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ca2 indicator fluo 3 am - by Bioz Stars, 2020-11
    99/100 stars

    Images

    Related Articles

    Flow Cytometry:

    Article Title: The inhibition of KDM2B promotes the differentiation of basal-like breast cancer cells via the posttranslational destabilization of SLUG
    Article Snippet: .. Intracellular calcium levels Cells loaded with Fluo3-AM (Molecular probes, Cat. #F1241) were trypsinized and the intensity of fluorescence induced by Ca2+ binding was measured by flow cytometry. .. Calpain activity assay Cells were lysed in an EDTA-free lysis buffer containing 20 mM Tris-HCl pH=7.5 and supplemented with a protease inhibitor cocktail (Sigma-Aldrich, Cat. # 4693159001).

    Fluorescence:

    Article Title: The inhibition of KDM2B promotes the differentiation of basal-like breast cancer cells via the posttranslational destabilization of SLUG
    Article Snippet: .. Intracellular calcium levels Cells loaded with Fluo3-AM (Molecular probes, Cat. #F1241) were trypsinized and the intensity of fluorescence induced by Ca2+ binding was measured by flow cytometry. .. Calpain activity assay Cells were lysed in an EDTA-free lysis buffer containing 20 mM Tris-HCl pH=7.5 and supplemented with a protease inhibitor cocktail (Sigma-Aldrich, Cat. # 4693159001).

    Stable Transfection:

    Article Title: Differences in the Signaling Pathways of ?1A- and ?1B-Adrenoceptors Are Related to Different Endosomal Targeting
    Article Snippet: .. Real-time imaging of calcium signal To monitor intracellular calcium concentration, HEK293 cells stably expressing the N-terminal VSV-G tagged or untagged human α1A - and α1B -AR subtypes were plated onto poly-L-Lysine coated sterile coverslips 48h before experimentation, washed three times with cold Krebs-Ringer-Hepes buffer (KRH, 120 mM NaCl, 25 mM HEPES, 4.8 mM KCl, 1.2 mM MgSO4 and 1.3 mM CaCl2 at pH 7.4) at 4°C and incubated for 2 hours with the fluorescent calcium chelator FLUO-3-AM (5 µM) (Invitrogen, Carlsbad CA, USA) in KRH at 5% CO2 and 37°C. .. Then, cells were washed once with KRH and mounted into a flow chamber bath placed on the microscope stage in a 95% air and 5% CO2 atmosphere at 37°C as has been described above.

    Concentration Assay:

    Article Title: Differences in the Signaling Pathways of ?1A- and ?1B-Adrenoceptors Are Related to Different Endosomal Targeting
    Article Snippet: .. Real-time imaging of calcium signal To monitor intracellular calcium concentration, HEK293 cells stably expressing the N-terminal VSV-G tagged or untagged human α1A - and α1B -AR subtypes were plated onto poly-L-Lysine coated sterile coverslips 48h before experimentation, washed three times with cold Krebs-Ringer-Hepes buffer (KRH, 120 mM NaCl, 25 mM HEPES, 4.8 mM KCl, 1.2 mM MgSO4 and 1.3 mM CaCl2 at pH 7.4) at 4°C and incubated for 2 hours with the fluorescent calcium chelator FLUO-3-AM (5 µM) (Invitrogen, Carlsbad CA, USA) in KRH at 5% CO2 and 37°C. .. Then, cells were washed once with KRH and mounted into a flow chamber bath placed on the microscope stage in a 95% air and 5% CO2 atmosphere at 37°C as has been described above.

    Article Title: The effect of Shenmai injection on the proliferation of Rat airway smooth muscle cells in asthma and underlying mechanism
    Article Snippet: .. Determination of the intracellular Ca2+ concentration ( [Ca2+ ]i ) The [Ca2+ ]i was measured using the intracellular calcium indicator Fluo-3/AM (Invitrogen, USA). ..

    Incubation:

    Article Title: An apicosome initiates self-organizing morphogenesis of human pluripotent stem cells
    Article Snippet: .. After 16–20 h, cells were rinsed once with DMEM/F12 before incubation in mTeSR containing 1.5 μM Fluo-3-AM (F1242; Invitrogen) for 30 min. .. Cells were then washed with DMEM/F12 to remove any residual membrane bound dyes and incubated for an additional 30 min in mTeSR alone to allow de-esterification.

    Article Title: Differences in the Signaling Pathways of ?1A- and ?1B-Adrenoceptors Are Related to Different Endosomal Targeting
    Article Snippet: .. Real-time imaging of calcium signal To monitor intracellular calcium concentration, HEK293 cells stably expressing the N-terminal VSV-G tagged or untagged human α1A - and α1B -AR subtypes were plated onto poly-L-Lysine coated sterile coverslips 48h before experimentation, washed three times with cold Krebs-Ringer-Hepes buffer (KRH, 120 mM NaCl, 25 mM HEPES, 4.8 mM KCl, 1.2 mM MgSO4 and 1.3 mM CaCl2 at pH 7.4) at 4°C and incubated for 2 hours with the fluorescent calcium chelator FLUO-3-AM (5 µM) (Invitrogen, Carlsbad CA, USA) in KRH at 5% CO2 and 37°C. .. Then, cells were washed once with KRH and mounted into a flow chamber bath placed on the microscope stage in a 95% air and 5% CO2 atmosphere at 37°C as has been described above.

    Article Title: Human Cystic Fibrosis Macrophages Have Defective Calcium-Dependent PKC Activation of the NADPH Oxidase, an Effect Augmented by Burkholderia cenocepacia
    Article Snippet: .. The MDMs were incubated at 37°C for 30 min with 4µg/mL fluorescent dye Fluo-3 AM (Life Technologies, F1242), washed twice and re-suspended in cell loading HBSS medium at 1E7 cells/mL. .. MDMs were stimulated with 1µg/mL Ionomycin (Sigma-Aldrich, 124222), 1µM Platelet Activating Factor (PAF, Sigma-Aldrich, P4904), bacteria (MOI 10) or PMA (Calbiochem, 524400).

    other:

    Article Title: Anti-Inflammatory Effects of Secondary Metabolites of Marine Pseudomonas sp. in Human Neutrophils Are through Inhibiting P38 MAPK, JNK, and Calcium Pathways
    Article Snippet: Reagents Fluo-3/AM was obtained from Molecular Probes (Eugene, OR, USA).

    Imaging:

    Article Title: Differences in the Signaling Pathways of ?1A- and ?1B-Adrenoceptors Are Related to Different Endosomal Targeting
    Article Snippet: .. Real-time imaging of calcium signal To monitor intracellular calcium concentration, HEK293 cells stably expressing the N-terminal VSV-G tagged or untagged human α1A - and α1B -AR subtypes were plated onto poly-L-Lysine coated sterile coverslips 48h before experimentation, washed three times with cold Krebs-Ringer-Hepes buffer (KRH, 120 mM NaCl, 25 mM HEPES, 4.8 mM KCl, 1.2 mM MgSO4 and 1.3 mM CaCl2 at pH 7.4) at 4°C and incubated for 2 hours with the fluorescent calcium chelator FLUO-3-AM (5 µM) (Invitrogen, Carlsbad CA, USA) in KRH at 5% CO2 and 37°C. .. Then, cells were washed once with KRH and mounted into a flow chamber bath placed on the microscope stage in a 95% air and 5% CO2 atmosphere at 37°C as has been described above.

    Expressing:

    Article Title: Differences in the Signaling Pathways of ?1A- and ?1B-Adrenoceptors Are Related to Different Endosomal Targeting
    Article Snippet: .. Real-time imaging of calcium signal To monitor intracellular calcium concentration, HEK293 cells stably expressing the N-terminal VSV-G tagged or untagged human α1A - and α1B -AR subtypes were plated onto poly-L-Lysine coated sterile coverslips 48h before experimentation, washed three times with cold Krebs-Ringer-Hepes buffer (KRH, 120 mM NaCl, 25 mM HEPES, 4.8 mM KCl, 1.2 mM MgSO4 and 1.3 mM CaCl2 at pH 7.4) at 4°C and incubated for 2 hours with the fluorescent calcium chelator FLUO-3-AM (5 µM) (Invitrogen, Carlsbad CA, USA) in KRH at 5% CO2 and 37°C. .. Then, cells were washed once with KRH and mounted into a flow chamber bath placed on the microscope stage in a 95% air and 5% CO2 atmosphere at 37°C as has been described above.

    Binding Assay:

    Article Title: The inhibition of KDM2B promotes the differentiation of basal-like breast cancer cells via the posttranslational destabilization of SLUG
    Article Snippet: .. Intracellular calcium levels Cells loaded with Fluo3-AM (Molecular probes, Cat. #F1241) were trypsinized and the intensity of fluorescence induced by Ca2+ binding was measured by flow cytometry. .. Calpain activity assay Cells were lysed in an EDTA-free lysis buffer containing 20 mM Tris-HCl pH=7.5 and supplemented with a protease inhibitor cocktail (Sigma-Aldrich, Cat. # 4693159001).

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  • 99
    Thermo Fisher ca2
    HS exposure causes Ca 2+ loss. (A) Relative [Ca 2+ ] i levels of RAW264.7 MΦs. Traces of Fura-2–loaded RAW264.7 MΦs stimulated ± HS at t = 10 s (mean ± SD; n = 6). Where indicated, Tg was added (means ± SD; n = 2). (B) As in (A), but RAW264.7 MΦs were stimulated with LPS ± HS at t = 10 s (mean ± SD; n = 5). Where indicated, Tg was added (means ± SD; n = 2). (C) As in (A), but relative [Ca 2+ ] i levels were assessed in Fluo-3/Fura-Red–loaded MΦs (means ± SD; n = 6). (D) As in (B), but relative [Ca 2+ ] i levels were assessed in Fluo-3/Fura-Red–loaded MΦs (means ± SD; n = 8). For numerical raw data, please see S1 Data . <t>[Ca2+]</t> i , intracellular Ca 2+ in situ; HS, high salt; LPS, lipopolysaccharide; MΦ, monocyte/macrophage-like cell; Tg, thapsigargin.
    Ca2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ca2/product/Thermo Fisher
    Average 99 stars, based on 304 article reviews
    Price from $9.99 to $1999.99
    ca2 - by Bioz Stars, 2020-11
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    88
    Thermo Fisher fluo 4 dextran
    HS exposure causes Ca 2+ loss. (A) Relative [Ca 2+ ] i levels of RAW264.7 MΦs. Traces of Fura-2–loaded RAW264.7 MΦs stimulated ± HS at t = 10 s (mean ± SD; n = 6). Where indicated, Tg was added (means ± SD; n = 2). (B) As in (A), but RAW264.7 MΦs were stimulated with LPS ± HS at t = 10 s (mean ± SD; n = 5). Where indicated, Tg was added (means ± SD; n = 2). (C) As in (A), but relative [Ca 2+ ] i levels were assessed in Fluo-3/Fura-Red–loaded MΦs (means ± SD; n = 6). (D) As in (B), but relative [Ca 2+ ] i levels were assessed in Fluo-3/Fura-Red–loaded MΦs (means ± SD; n = 8). For numerical raw data, please see S1 Data . <t>[Ca2+]</t> i , intracellular Ca 2+ in situ; HS, high salt; LPS, lipopolysaccharide; MΦ, monocyte/macrophage-like cell; Tg, thapsigargin.
    Fluo 4 Dextran, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluo 4 dextran/product/Thermo Fisher
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    fluo 4 dextran - by Bioz Stars, 2020-11
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    Image Search Results


    HS exposure causes Ca 2+ loss. (A) Relative [Ca 2+ ] i levels of RAW264.7 MΦs. Traces of Fura-2–loaded RAW264.7 MΦs stimulated ± HS at t = 10 s (mean ± SD; n = 6). Where indicated, Tg was added (means ± SD; n = 2). (B) As in (A), but RAW264.7 MΦs were stimulated with LPS ± HS at t = 10 s (mean ± SD; n = 5). Where indicated, Tg was added (means ± SD; n = 2). (C) As in (A), but relative [Ca 2+ ] i levels were assessed in Fluo-3/Fura-Red–loaded MΦs (means ± SD; n = 6). (D) As in (B), but relative [Ca 2+ ] i levels were assessed in Fluo-3/Fura-Red–loaded MΦs (means ± SD; n = 8). For numerical raw data, please see S1 Data . [Ca2+] i , intracellular Ca 2+ in situ; HS, high salt; LPS, lipopolysaccharide; MΦ, monocyte/macrophage-like cell; Tg, thapsigargin.

    Journal: PLoS Biology

    Article Title: NCX1 represents an ionic Na+ sensing mechanism in macrophages

    doi: 10.1371/journal.pbio.3000722

    Figure Lengend Snippet: HS exposure causes Ca 2+ loss. (A) Relative [Ca 2+ ] i levels of RAW264.7 MΦs. Traces of Fura-2–loaded RAW264.7 MΦs stimulated ± HS at t = 10 s (mean ± SD; n = 6). Where indicated, Tg was added (means ± SD; n = 2). (B) As in (A), but RAW264.7 MΦs were stimulated with LPS ± HS at t = 10 s (mean ± SD; n = 5). Where indicated, Tg was added (means ± SD; n = 2). (C) As in (A), but relative [Ca 2+ ] i levels were assessed in Fluo-3/Fura-Red–loaded MΦs (means ± SD; n = 6). (D) As in (B), but relative [Ca 2+ ] i levels were assessed in Fluo-3/Fura-Red–loaded MΦs (means ± SD; n = 8). For numerical raw data, please see S1 Data . [Ca2+] i , intracellular Ca 2+ in situ; HS, high salt; LPS, lipopolysaccharide; MΦ, monocyte/macrophage-like cell; Tg, thapsigargin.

    Article Snippet: Intracellular Ca2+ measurements using flow cytometry RAW264.7 MΦs were stained with the Ca2+-sensitive dyes Fluo-3 (Thermo Fisher Scientific; #F-1241) and Fura-Red (Thermo Fisher Scientific; #F-3021) [ ] for 20 min at room temperature in Tyrode solution containing Pluronic.

    Techniques: In Situ