Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Efficient stimulus-secretion coupling at ribbon synapses requires RIM-binding protein tethering of L-type Ca 2+ channels

doi: 10.1073/pnas.1702991114

Figure Lengend Snippet: Antibodies used in the current study with corresponding dilutions and origin

Article Snippet: A list of antibodies used in the current study with corresponding dilutions and origin are presented in . table ft1 table-wrap mode="anchored" t5 Table S1. caption a7 Antigen Antibody Dilution Active zone proteins RBP-1 (RIM-binding protein-1) 316003 (SySy) 1:1,000 RBP-2 (RIM-binding protein-2) 316103 (SySy) 1:1,000 RBP-2 (RIM-binding protein-2) 4193 (custom) 1:1,000 RIM 1 (RIM1 central domains) R809 (custom) 1:2,000 RIM 1/2 (1α/2α N terminus + rabphillin) U1565 (custom) 1:2,000 Liprin α3 4396 (custom) 1:5,000 ELKS 1/2aB 4790 (custom) 1:500 CASK 75–000 (Neuromab) 1:1,000 Mint1 P932 (custom) 1;1000 Veli1,2,3 T813 (custom) 1:1,000 Munc13-1 126103 (SySy) 1:1,000 Ribeye Maxeiner et al. ( 32 ) 1:1,000 Ca 2+ channels Ca 2+ v1.2-α1C voltage-gated channel ACC-003 (Alomone) 1:200 Ca 2+ v1.3-α1D voltage-gated channel ACC-005 (Alomone) 1:200 Ca 2+ v1.4-α1D voltage-gated channel Gift from Frank Schmitz 1:1,000 Ca 2+ v-α2δ1 voltage-gated channel ACC-015 (Alomone) 1:500 Ca 2+ v-α2δ2 voltage-gated channel ACC-102 (Alomone) 1:500 Ca 2+ v-α2δ3 voltage-gated channel ACC-103 (Alomone) 1:500 Ca 2+ v-α2δ4 voltage-gated channel ACC-104 (Alomone) 1:500 Ca 2+ β1 voltage-gated channel ACC-106 (Alomone) 1:250 Ca 2+ β2 voltage-gated channel ACC-105 (Alomone) 1:250 Ca 2+ β3 voltage-gated channel ACC-108 (Alomone) 1:250 Ca 2+ β4 voltage-gated channel 75–054 (Neuromab) 1:250 Exocytosis proteins Syntaxin 1 HPC-1 (custom) 1:500 SNAP 25 71.1 (custom) 1:2,000 Synaptobrevin 2 69.1 (custom) 1:2,500 Synaptotagmin 1 41.1 (custom) 1:1,000 Loading control TUJ1 T2200 (Sigma) 1:5,000 Open in a separate window Antibodies used in the current study with corresponding dilutions and origin

Techniques:

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Efficient stimulus-secretion coupling at ribbon synapses requires RIM-binding protein tethering of L-type Ca 2+ channels

doi: 10.1073/pnas.1702991114

Figure Lengend Snippet: Antibodies used in the current study with corresponding dilutions and origin

Article Snippet: A list of antibodies used in the current study with corresponding dilutions and origin are presented in . table ft1 table-wrap mode="anchored" t5 Table S1. caption a7 Antigen Antibody Dilution Active zone proteins RBP-1 (RIM-binding protein-1) 316003 (SySy) 1:1,000 RBP-2 (RIM-binding protein-2) 316103 (SySy) 1:1,000 RBP-2 (RIM-binding protein-2) 4193 (custom) 1:1,000 RIM 1 (RIM1 central domains) R809 (custom) 1:2,000 RIM 1/2 (1α/2α N terminus + rabphillin) U1565 (custom) 1:2,000 Liprin α3 4396 (custom) 1:5,000 ELKS 1/2aB 4790 (custom) 1:500 CASK 75–000 (Neuromab) 1:1,000 Mint1 P932 (custom) 1;1000 Veli1,2,3 T813 (custom) 1:1,000 Munc13-1 126103 (SySy) 1:1,000 Ribeye Maxeiner et al. ( 32 ) 1:1,000 Ca 2+ channels Ca 2+ v1.2-α1C voltage-gated channel ACC-003 (Alomone) 1:200 Ca 2+ v1.3-α1D voltage-gated channel ACC-005 (Alomone) 1:200 Ca 2+ v1.4-α1D voltage-gated channel Gift from Frank Schmitz 1:1,000 Ca 2+ v-α2δ1 voltage-gated channel ACC-015 (Alomone) 1:500 Ca 2+ v-α2δ2 voltage-gated channel ACC-102 (Alomone) 1:500 Ca 2+ v-α2δ3 voltage-gated channel ACC-103 (Alomone) 1:500 Ca 2+ v-α2δ4 voltage-gated channel ACC-104 (Alomone) 1:500 Ca 2+ β1 voltage-gated channel ACC-106 (Alomone) 1:250 Ca 2+ β2 voltage-gated channel ACC-105 (Alomone) 1:250 Ca 2+ β3 voltage-gated channel ACC-108 (Alomone) 1:250 Ca 2+ β4 voltage-gated channel 75–054 (Neuromab) 1:250 Exocytosis proteins Syntaxin 1 HPC-1 (custom) 1:500 SNAP 25 71.1 (custom) 1:2,000 Synaptobrevin 2 69.1 (custom) 1:2,500 Synaptotagmin 1 41.1 (custom) 1:1,000 Loading control TUJ1 T2200 (Sigma) 1:5,000 Open in a separate window Antibodies used in the current study with corresponding dilutions and origin Electron Microscopy.

Techniques: