c57 bl6 mice  (Millipore)


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    Structured Review

    Millipore c57 bl6 mice
    Effect of angiotensin II induced hypertension on plasma ADMA and creatinine levels. Systolic blood pressure (A), ADMA (C) and creatinine (E) in normotensive and hypertensive <t>C57/BL6</t> mice on a control diet. Data are mean±SEM, n=5–12. Systolic blood pressure (B), ADMA (D) and creatinine (F) in normotensive and hypertensive C57/BL6 mice on a celecoxib diet. Data are mean±SEM, n=3–15; *p
    C57 Bl6 Mice, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c57 bl6 mice/product/Millipore
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    c57 bl6 mice - by Bioz Stars, 2020-10
    99/100 stars

    Images

    1) Product Images from "Cyclooxygenase-2, asymmetric dimethylarginine and the cardiovascular hazard from NSAIDs."

    Article Title: Cyclooxygenase-2, asymmetric dimethylarginine and the cardiovascular hazard from NSAIDs.

    Journal: Circulation

    doi: 10.1161/CIRCULATIONAHA.118.033540

    Effect of angiotensin II induced hypertension on plasma ADMA and creatinine levels. Systolic blood pressure (A), ADMA (C) and creatinine (E) in normotensive and hypertensive C57/BL6 mice on a control diet. Data are mean±SEM, n=5–12. Systolic blood pressure (B), ADMA (D) and creatinine (F) in normotensive and hypertensive C57/BL6 mice on a celecoxib diet. Data are mean±SEM, n=3–15; *p
    Figure Legend Snippet: Effect of angiotensin II induced hypertension on plasma ADMA and creatinine levels. Systolic blood pressure (A), ADMA (C) and creatinine (E) in normotensive and hypertensive C57/BL6 mice on a control diet. Data are mean±SEM, n=5–12. Systolic blood pressure (B), ADMA (D) and creatinine (F) in normotensive and hypertensive C57/BL6 mice on a celecoxib diet. Data are mean±SEM, n=3–15; *p

    Techniques Used: Mouse Assay

    2) Product Images from "Effects of In Utero Exposure to Bisphenol A or Diethylstilbestrol on the Adult Male Reproductive System"

    Article Title: Effects of In Utero Exposure to Bisphenol A or Diethylstilbestrol on the Adult Male Reproductive System

    Journal: Birth defects research. Part B, Developmental and reproductive toxicology

    doi: 10.1002/bdrb.20336

    Germ cell apoptosis in adult C57/Bl6 male mice following in utero exposure to sesame oil, 50 μg/kg BPA, 1,000 μg/kg BPA, or 2 μg/kg DES The apoptotic incidence in seminiferous tubules was quantified by using TUNEL staining (a). The percent of TUNEL-positive tubules with greater than 3 apoptotic cells is represented as mean + standard deviation. No significant differences in apoptotic incidence were found for BPA or DES treated testes. Statistical analyses were conducted using one-way ANOVA followed by a Tukey HSD test for BPA and a two-tailed t-test for DES. n=6 per treatment group, with each n representing a different litter. Representative images of TUNEL staining in the testis for sesame oil (b), BPA 50 μg/kg (c), BPA 1,000 μg/kg (d), and DES 2 μg/kg (e). Scale bars indicate 1,000 μm.
    Figure Legend Snippet: Germ cell apoptosis in adult C57/Bl6 male mice following in utero exposure to sesame oil, 50 μg/kg BPA, 1,000 μg/kg BPA, or 2 μg/kg DES The apoptotic incidence in seminiferous tubules was quantified by using TUNEL staining (a). The percent of TUNEL-positive tubules with greater than 3 apoptotic cells is represented as mean + standard deviation. No significant differences in apoptotic incidence were found for BPA or DES treated testes. Statistical analyses were conducted using one-way ANOVA followed by a Tukey HSD test for BPA and a two-tailed t-test for DES. n=6 per treatment group, with each n representing a different litter. Representative images of TUNEL staining in the testis for sesame oil (b), BPA 50 μg/kg (c), BPA 1,000 μg/kg (d), and DES 2 μg/kg (e). Scale bars indicate 1,000 μm.

    Techniques Used: Mouse Assay, In Utero, TUNEL Assay, Staining, Standard Deviation, Two Tailed Test

    3) Product Images from "Impaired chemokine-induced migration during T-cell development in the absence of Jak 3"

    Article Title: Impaired chemokine-induced migration during T-cell development in the absence of Jak 3

    Journal: Immunology

    doi: 10.1111/j.1365-2567.2004.01863.x

    CCL25 and CXCL12 induce tyrosine phosphorylation of Jak 3. A representative experiment of n = 5 is shown. Thymocytes (30 × 10 6 ) from 4–10-week-old C57/BL6 mice were stimulated with CXCL12 or CCL25 for 15 and 60 seconds, and Jak 3 phosphorylation was analysed after immunoprecipitation and blotting with the phosphotyrosine antibody, 4G10 (top panel). As a positive control, thymocytes were stimulated with recombinant interleukin-7 (IL-7) (100 ng/ml) After stripping, the membrane was reprobed with Jak 3 polyclonal antisera (888) (bottom panel). Densitometric analysis was performed on phosphotyrosine and Jak 3 blots, and the phosphorylation level of Jak 3 was calculated as the ratio of the densitometric intensities of the anti-phosphotyrosine signal to the anti-Jak 3 signal of the respective bands. The data were normalized to the values obtained with thymocytes treated in media alone.
    Figure Legend Snippet: CCL25 and CXCL12 induce tyrosine phosphorylation of Jak 3. A representative experiment of n = 5 is shown. Thymocytes (30 × 10 6 ) from 4–10-week-old C57/BL6 mice were stimulated with CXCL12 or CCL25 for 15 and 60 seconds, and Jak 3 phosphorylation was analysed after immunoprecipitation and blotting with the phosphotyrosine antibody, 4G10 (top panel). As a positive control, thymocytes were stimulated with recombinant interleukin-7 (IL-7) (100 ng/ml) After stripping, the membrane was reprobed with Jak 3 polyclonal antisera (888) (bottom panel). Densitometric analysis was performed on phosphotyrosine and Jak 3 blots, and the phosphorylation level of Jak 3 was calculated as the ratio of the densitometric intensities of the anti-phosphotyrosine signal to the anti-Jak 3 signal of the respective bands. The data were normalized to the values obtained with thymocytes treated in media alone.

    Techniques Used: Mouse Assay, Immunoprecipitation, Positive Control, Recombinant, Stripping Membranes

    Haematopoietic bone marrow progenitors respond to CXCL12 and CCL25. Total bone marrow cells from wild-type and Jak 3 –/– mice were stained with Sca-1- and c-kit-specific antibodies to characterize the percentages of haematopoietic bone marrow progenitors in these mice. A representative experiment (out of five) is shown in (a). Total bone marrow cells from C57/BL6 mice were subjected to chemotaxis assays using a transwell technique (b). As shown, a significant percentage of the migrated cells towards CXCL12 and CCL25 (10 ng/ml of chemokine), were positive for Sca-1 and c-kit. A representative experiment (of a total of three) is shown.
    Figure Legend Snippet: Haematopoietic bone marrow progenitors respond to CXCL12 and CCL25. Total bone marrow cells from wild-type and Jak 3 –/– mice were stained with Sca-1- and c-kit-specific antibodies to characterize the percentages of haematopoietic bone marrow progenitors in these mice. A representative experiment (out of five) is shown in (a). Total bone marrow cells from C57/BL6 mice were subjected to chemotaxis assays using a transwell technique (b). As shown, a significant percentage of the migrated cells towards CXCL12 and CCL25 (10 ng/ml of chemokine), were positive for Sca-1 and c-kit. A representative experiment (of a total of three) is shown.

    Techniques Used: Mouse Assay, Staining, Chemotaxis Assay

    4) Product Images from "Cyclooxygenase-2, asymmetric dimethylarginine and the cardiovascular hazard from NSAIDs."

    Article Title: Cyclooxygenase-2, asymmetric dimethylarginine and the cardiovascular hazard from NSAIDs.

    Journal: Circulation

    doi: 10.1161/CIRCULATIONAHA.118.033540

    Effect of angiotensin II induced hypertension on plasma ADMA and creatinine levels. Systolic blood pressure (A), ADMA (C) and creatinine (E) in normotensive and hypertensive C57/BL6 mice on a control diet. Data are mean±SEM, n=5–12. Systolic blood pressure (B), ADMA (D) and creatinine (F) in normotensive and hypertensive C57/BL6 mice on a celecoxib diet. Data are mean±SEM, n=3–15; *p
    Figure Legend Snippet: Effect of angiotensin II induced hypertension on plasma ADMA and creatinine levels. Systolic blood pressure (A), ADMA (C) and creatinine (E) in normotensive and hypertensive C57/BL6 mice on a control diet. Data are mean±SEM, n=5–12. Systolic blood pressure (B), ADMA (D) and creatinine (F) in normotensive and hypertensive C57/BL6 mice on a celecoxib diet. Data are mean±SEM, n=3–15; *p

    Techniques Used: Mouse Assay

    Related Articles

    Transfection:

    Article Title: The Ectodomain of a Novel Member of the Immunoglobulin Subfamily Related to the Poliovirus Receptor Has the Attributes of a Bona Fide Receptor for Herpes Simplex Virus Types 1 and 2 in Human Cells
    Article Snippet: .. J1.1-2 cells in glass coverslips were transfected with pCF18, fixed with methanol 30 h later, incubated with biotinylated recombinant gD-1(Δ290–299t) (0.1 μg in 30 μl/coverslip) ( ) for 1 h at room temperature followed by Extravidin-TRITC (Sigma) (1:100 in phosphate-buffered saline containing 20% fetal calf serum) for 1 h at room temperature. .. For biotinylation, 7 μg of gD was incubated with 1 μg of Immunopure sulfo-NHS-biotin (Pierce) in 50 mM sodium bicarbonate buffer (pH 8.5) for 2 h on ice.

    Binding Assay:

    Article Title: The OmpL37 Surface-Exposed Protein Is Expressed by Pathogenic Leptospira during Infection and Binds Skin and Vascular Elastin
    Article Snippet: .. Enzyme-linked immunosorbent assay (ELISA) of binding to host ligands by OmpL proteins Host ligands included human plasma fibronectin (Sigma-Aldrich), human plasma fibronectin 30-kDa proteolytic fragment (heparin-binding domain, Sigma-Aldrich), human plasma fibronectin 45-kDa proteolytic fragment (gelatin-binding domain, Sigma-Aldrich), human fibroblast fibronectin (Calbiochem, La Jolla, CA), human plasma fibrinogen (HYPHEN BioMed, France), human plasma fibrinogen fragment D (HYPHEN BioMed), murine laminin (Sigma-Aldrich), bovine skin collagen type I (Sigma-Aldrich), human placenta collagen type III (Sigma-Aldrich), human placenta collagen type IV (Sigma-Aldrich), soluble human skin elastin (Elastin Products Company, Owensville, MO), soluble human aorta elastin (Sigma-Aldrich), bovine kidney heparan sulfate (Sigma-Aldrich), shark cartilage chondroitin sulfate (Sigma-Aldrich), fetal calf serum fetuin (Sigma-Aldrich), and bovine serum albumin (BSA, Sigma-Aldrich). .. Ultra-high binding Immulon 4HBX microtiter plates (Thermo Scientific) were coated with 1 µg of host ligand in 0.1 ml of phosphate buffered saline (PBS), pH 7.2, and incubated overnight at 4°C.

    Cell Culture:

    Article Title: HSP90 promotes Burkitt lymphoma cell survival by maintaining tonic B-cell receptor signaling
    Article Snippet: .. Stable isotope labeling with amino acids in cell culture (SILAC) of DG75 and Daudi cells was performed by culturing cells in SILAC-RPMI 1640 medium devoid of arginine and lysine (Pierce) supplemented with 10% (DG75) or 20% (Daudi) dialyzed fetal calf serum (Sigma-Aldrich, Taufkirchen, Germany), penicillin/streptomycin (Invitrogen), and the respective SILAC amino acids (all from Cambridge Isotopes, Tewksbury, MA). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: The OmpL37 Surface-Exposed Protein Is Expressed by Pathogenic Leptospira during Infection and Binds Skin and Vascular Elastin
    Article Snippet: .. Enzyme-linked immunosorbent assay (ELISA) of binding to host ligands by OmpL proteins Host ligands included human plasma fibronectin (Sigma-Aldrich), human plasma fibronectin 30-kDa proteolytic fragment (heparin-binding domain, Sigma-Aldrich), human plasma fibronectin 45-kDa proteolytic fragment (gelatin-binding domain, Sigma-Aldrich), human fibroblast fibronectin (Calbiochem, La Jolla, CA), human plasma fibrinogen (HYPHEN BioMed, France), human plasma fibrinogen fragment D (HYPHEN BioMed), murine laminin (Sigma-Aldrich), bovine skin collagen type I (Sigma-Aldrich), human placenta collagen type III (Sigma-Aldrich), human placenta collagen type IV (Sigma-Aldrich), soluble human skin elastin (Elastin Products Company, Owensville, MO), soluble human aorta elastin (Sigma-Aldrich), bovine kidney heparan sulfate (Sigma-Aldrich), shark cartilage chondroitin sulfate (Sigma-Aldrich), fetal calf serum fetuin (Sigma-Aldrich), and bovine serum albumin (BSA, Sigma-Aldrich). .. Ultra-high binding Immulon 4HBX microtiter plates (Thermo Scientific) were coated with 1 µg of host ligand in 0.1 ml of phosphate buffered saline (PBS), pH 7.2, and incubated overnight at 4°C.

    Incubation:

    Article Title: The Ectodomain of a Novel Member of the Immunoglobulin Subfamily Related to the Poliovirus Receptor Has the Attributes of a Bona Fide Receptor for Herpes Simplex Virus Types 1 and 2 in Human Cells
    Article Snippet: .. J1.1-2 cells in glass coverslips were transfected with pCF18, fixed with methanol 30 h later, incubated with biotinylated recombinant gD-1(Δ290–299t) (0.1 μg in 30 μl/coverslip) ( ) for 1 h at room temperature followed by Extravidin-TRITC (Sigma) (1:100 in phosphate-buffered saline containing 20% fetal calf serum) for 1 h at room temperature. .. For biotinylation, 7 μg of gD was incubated with 1 μg of Immunopure sulfo-NHS-biotin (Pierce) in 50 mM sodium bicarbonate buffer (pH 8.5) for 2 h on ice.

    other:

    Article Title: Correlation between NK function and response to trastuzumab in metastatic breast cancer patients
    Article Snippet: Culture medium was RPMI 1640 (Life Technologies Ltd, Paisley, Scotland, UK) supplemented with 20% (SKBR3 and MCF7) or 10% (K562) heat inactivated fetal calf serum (FCS), 1% L-glutamine, 1% penicillin and streptomycin (Sigma-Aldrich, Milan, Italy).

    Labeling:

    Article Title: HSP90 promotes Burkitt lymphoma cell survival by maintaining tonic B-cell receptor signaling
    Article Snippet: .. Stable isotope labeling with amino acids in cell culture (SILAC) of DG75 and Daudi cells was performed by culturing cells in SILAC-RPMI 1640 medium devoid of arginine and lysine (Pierce) supplemented with 10% (DG75) or 20% (Daudi) dialyzed fetal calf serum (Sigma-Aldrich, Taufkirchen, Germany), penicillin/streptomycin (Invitrogen), and the respective SILAC amino acids (all from Cambridge Isotopes, Tewksbury, MA). ..

    Modification:

    Article Title: In Vivo Imaging of Physiological Angiogenesis from Immature to Preovulatory Ovarian Follicles
    Article Snippet: .. After laparotomy, donor ovaries were aseptically removed and placed in 30-mm-diameter Falcon plastic Petri dishes filled with 37°C warm Dulbecco’s modified Eagle’s medium (10% fetal calf serum, 0.1 mg/ml gentamicin), and the fluorescent vital dye bisbenzimide (200 μg/ml; Sigma). .. After removing the surrounding tissue, the ovaries were microdissected under a stereo microscope using 27-gauge needles.

    Recombinant:

    Article Title: The Ectodomain of a Novel Member of the Immunoglobulin Subfamily Related to the Poliovirus Receptor Has the Attributes of a Bona Fide Receptor for Herpes Simplex Virus Types 1 and 2 in Human Cells
    Article Snippet: .. J1.1-2 cells in glass coverslips were transfected with pCF18, fixed with methanol 30 h later, incubated with biotinylated recombinant gD-1(Δ290–299t) (0.1 μg in 30 μl/coverslip) ( ) for 1 h at room temperature followed by Extravidin-TRITC (Sigma) (1:100 in phosphate-buffered saline containing 20% fetal calf serum) for 1 h at room temperature. .. For biotinylation, 7 μg of gD was incubated with 1 μg of Immunopure sulfo-NHS-biotin (Pierce) in 50 mM sodium bicarbonate buffer (pH 8.5) for 2 h on ice.

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    Millipore c57 bl6 mice
    Effect of angiotensin II induced hypertension on plasma ADMA and creatinine levels. Systolic blood pressure (A), ADMA (C) and creatinine (E) in normotensive and hypertensive <t>C57/BL6</t> mice on a control diet. Data are mean±SEM, n=5–12. Systolic blood pressure (B), ADMA (D) and creatinine (F) in normotensive and hypertensive C57/BL6 mice on a celecoxib diet. Data are mean±SEM, n=3–15; *p
    C57 Bl6 Mice, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c57 bl6 mice/product/Millipore
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    c57 bl6 mice - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    85
    Millipore neurod2 smoa1 mice bearing tumors
    Treatment of medulloblastoma-bearing mice with PPARγ antagonist GW9662 prolongs survival and reduces glucose uptake in vivo. a FDG-PET scan of medulloblastoma-bearing <t>NeuroD2</t> - <t>SmoA1</t> mouse before and after treatment with the PPARγ antagonist GW9662 (10 mg/kg, intraperitoneal injection daily over the 8-study day period). b A representative Oil red O staining of medulloblastoma sections from an untreated mouse ( day 0 ) and a counterpart treated with GW9662 for 8 days. c Kaplan–Meyer survival curve of NeuroD2 -SmoA1 medulloblastoma-bearing mice in days commencing with initiation of treatment with DMSO or GW9662.; * P value
    Neurod2 Smoa1 Mice Bearing Tumors, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neurod2 smoa1 mice bearing tumors/product/Millipore
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    neurod2 smoa1 mice bearing tumors - by Bioz Stars, 2020-10
    85/100 stars
      Buy from Supplier

    Image Search Results


    Effect of angiotensin II induced hypertension on plasma ADMA and creatinine levels. Systolic blood pressure (A), ADMA (C) and creatinine (E) in normotensive and hypertensive C57/BL6 mice on a control diet. Data are mean±SEM, n=5–12. Systolic blood pressure (B), ADMA (D) and creatinine (F) in normotensive and hypertensive C57/BL6 mice on a celecoxib diet. Data are mean±SEM, n=3–15; *p

    Journal: Circulation

    Article Title: Cyclooxygenase-2, asymmetric dimethylarginine and the cardiovascular hazard from NSAIDs.

    doi: 10.1161/CIRCULATIONAHA.118.033540

    Figure Lengend Snippet: Effect of angiotensin II induced hypertension on plasma ADMA and creatinine levels. Systolic blood pressure (A), ADMA (C) and creatinine (E) in normotensive and hypertensive C57/BL6 mice on a control diet. Data are mean±SEM, n=5–12. Systolic blood pressure (B), ADMA (D) and creatinine (F) in normotensive and hypertensive C57/BL6 mice on a celecoxib diet. Data are mean±SEM, n=3–15; *p

    Article Snippet: Ind.Cox-2 KO, WT and C57/BL6 mice (on a control diet or a celecoxib diet for three weeks) at 12–14 week of age received Ang II (1500 μg/Kg per day, Calbiochem, Darmstadt, Germany) in saline by continuous-infusion for 2 weeks.

    Techniques: Mouse Assay

    CCL25 and CXCL12 induce tyrosine phosphorylation of Jak 3. A representative experiment of n = 5 is shown. Thymocytes (30 × 10 6 ) from 4–10-week-old C57/BL6 mice were stimulated with CXCL12 or CCL25 for 15 and 60 seconds, and Jak 3 phosphorylation was analysed after immunoprecipitation and blotting with the phosphotyrosine antibody, 4G10 (top panel). As a positive control, thymocytes were stimulated with recombinant interleukin-7 (IL-7) (100 ng/ml) After stripping, the membrane was reprobed with Jak 3 polyclonal antisera (888) (bottom panel). Densitometric analysis was performed on phosphotyrosine and Jak 3 blots, and the phosphorylation level of Jak 3 was calculated as the ratio of the densitometric intensities of the anti-phosphotyrosine signal to the anti-Jak 3 signal of the respective bands. The data were normalized to the values obtained with thymocytes treated in media alone.

    Journal: Immunology

    Article Title: Impaired chemokine-induced migration during T-cell development in the absence of Jak 3

    doi: 10.1111/j.1365-2567.2004.01863.x

    Figure Lengend Snippet: CCL25 and CXCL12 induce tyrosine phosphorylation of Jak 3. A representative experiment of n = 5 is shown. Thymocytes (30 × 10 6 ) from 4–10-week-old C57/BL6 mice were stimulated with CXCL12 or CCL25 for 15 and 60 seconds, and Jak 3 phosphorylation was analysed after immunoprecipitation and blotting with the phosphotyrosine antibody, 4G10 (top panel). As a positive control, thymocytes were stimulated with recombinant interleukin-7 (IL-7) (100 ng/ml) After stripping, the membrane was reprobed with Jak 3 polyclonal antisera (888) (bottom panel). Densitometric analysis was performed on phosphotyrosine and Jak 3 blots, and the phosphorylation level of Jak 3 was calculated as the ratio of the densitometric intensities of the anti-phosphotyrosine signal to the anti-Jak 3 signal of the respective bands. The data were normalized to the values obtained with thymocytes treated in media alone.

    Article Snippet: For inhibition experiments, thymocytes or bone marrow cells from C57/BL6 mice [8 × 106 cells/ml in RPMI-1640 + 10% fetal calf serum (FCS)] were resuspended in medium containing the specific Jak 3 inhibitor, WH1-P131 (30 µg/ml; Calbiochem , San Diego, CA), pertussis toxin (PTX, 200 ng/ml; Sigma Chemicals, St Louis, MO), or dimethylsulphoxide (DMSO) (3 µl), and incubated for 1 hr at 37°.

    Techniques: Mouse Assay, Immunoprecipitation, Positive Control, Recombinant, Stripping Membranes

    Haematopoietic bone marrow progenitors respond to CXCL12 and CCL25. Total bone marrow cells from wild-type and Jak 3 –/– mice were stained with Sca-1- and c-kit-specific antibodies to characterize the percentages of haematopoietic bone marrow progenitors in these mice. A representative experiment (out of five) is shown in (a). Total bone marrow cells from C57/BL6 mice were subjected to chemotaxis assays using a transwell technique (b). As shown, a significant percentage of the migrated cells towards CXCL12 and CCL25 (10 ng/ml of chemokine), were positive for Sca-1 and c-kit. A representative experiment (of a total of three) is shown.

    Journal: Immunology

    Article Title: Impaired chemokine-induced migration during T-cell development in the absence of Jak 3

    doi: 10.1111/j.1365-2567.2004.01863.x

    Figure Lengend Snippet: Haematopoietic bone marrow progenitors respond to CXCL12 and CCL25. Total bone marrow cells from wild-type and Jak 3 –/– mice were stained with Sca-1- and c-kit-specific antibodies to characterize the percentages of haematopoietic bone marrow progenitors in these mice. A representative experiment (out of five) is shown in (a). Total bone marrow cells from C57/BL6 mice were subjected to chemotaxis assays using a transwell technique (b). As shown, a significant percentage of the migrated cells towards CXCL12 and CCL25 (10 ng/ml of chemokine), were positive for Sca-1 and c-kit. A representative experiment (of a total of three) is shown.

    Article Snippet: For inhibition experiments, thymocytes or bone marrow cells from C57/BL6 mice [8 × 106 cells/ml in RPMI-1640 + 10% fetal calf serum (FCS)] were resuspended in medium containing the specific Jak 3 inhibitor, WH1-P131 (30 µg/ml; Calbiochem , San Diego, CA), pertussis toxin (PTX, 200 ng/ml; Sigma Chemicals, St Louis, MO), or dimethylsulphoxide (DMSO) (3 µl), and incubated for 1 hr at 37°.

    Techniques: Mouse Assay, Staining, Chemotaxis Assay

    Treatment of medulloblastoma-bearing mice with PPARγ antagonist GW9662 prolongs survival and reduces glucose uptake in vivo. a FDG-PET scan of medulloblastoma-bearing NeuroD2 - SmoA1 mouse before and after treatment with the PPARγ antagonist GW9662 (10 mg/kg, intraperitoneal injection daily over the 8-study day period). b A representative Oil red O staining of medulloblastoma sections from an untreated mouse ( day 0 ) and a counterpart treated with GW9662 for 8 days. c Kaplan–Meyer survival curve of NeuroD2 -SmoA1 medulloblastoma-bearing mice in days commencing with initiation of treatment with DMSO or GW9662.; * P value

    Journal: Acta Neuropathologica

    Article Title: Hedgehog-mediated regulation of PPAR? controls metabolic patterns in neural precursors and shh-driven medulloblastoma

    doi: 10.1007/s00401-012-0968-6

    Figure Lengend Snippet: Treatment of medulloblastoma-bearing mice with PPARγ antagonist GW9662 prolongs survival and reduces glucose uptake in vivo. a FDG-PET scan of medulloblastoma-bearing NeuroD2 - SmoA1 mouse before and after treatment with the PPARγ antagonist GW9662 (10 mg/kg, intraperitoneal injection daily over the 8-study day period). b A representative Oil red O staining of medulloblastoma sections from an untreated mouse ( day 0 ) and a counterpart treated with GW9662 for 8 days. c Kaplan–Meyer survival curve of NeuroD2 -SmoA1 medulloblastoma-bearing mice in days commencing with initiation of treatment with DMSO or GW9662.; * P value

    Article Snippet: Animal studies Wild type C57-BL6 mice and NeuroD2-SmoA1 mice bearing tumors were administered 10 % dimethyl sulfoxide (DMSO, control), olomoucine (Calbiochem) at 6 mg/kg daily or GW9662 (Cayman Chemicals) at 10 mg/kg daily by intraperitoneal (i.p.) injection.

    Techniques: Mouse Assay, In Vivo, Positron Emission Tomography, Injection, Staining

    Shh-induced mouse medulloblastomas have robust lipogenesis, increased levels of E2F1 and PPARγ and cell proliferation. a Triglyceride accumulation was analyzed in a NeuroD2 -SmoA1 medulloblastoma using Oil Red O staining; neutral lipids appear as red droplets. NeuroD2 -SmoA1 medulloblastoma ( top row ) and adjacent non-tumorous cerebellum ( bottom row ) were subjected to immunofluorescence analyses for E2F1, PPARγ, and the proliferation marker Ki67. Primary and secondary antibodies were used at concentrations of 1:100. Leftmost panel shows hematoxylin and eosin staining (H E) staining of the tumor and cerebellar molecular layer ( mol ) and inner granule layer ( IGL ). The non-tumorous cerebellum immunofluorescence focuses on the boundary between the IGL and molecular layer demonstrated in the H E staining. Magnification: ×1.25 ( leftmost panel ), ×40 ( other images ). Bars 16 μM. Panels demonstrate the typical outcome from 5 different tumor and adjoining cerebellum specimens all paraffin embedded, sectioned, and subjected to immunofluorescence for the aforementioned antibodies. Additionally, the Oil red oil represents a typical outcome based on the staining of frozen sections from 5 different specimens. b Protein lysates were prepared from NeuroD2 -SmoA1 medulloblastoma and normal cerebellum adjacent to the tumor, and then analyzed by western blotting for PPARγ, lipogenic marker (FASN) and proliferation markers (E2F1 and cyclin D2). 40 μg protein/lane was loaded. Data presented are typical for all 5 sets of tumor and cerebellum samples that were studied. c Western blot analysis of proteins regulating proliferation and lipogenesis in adjacent non-tumor cerebellum or medulloblastomas from NeuroD2 -SmoA1 mice treated with DMSO (−) or cdk inhibitor olomoucine (+). The results shown represent a typical outcome amongst the control and experimental groups of mice

    Journal: Acta Neuropathologica

    Article Title: Hedgehog-mediated regulation of PPAR? controls metabolic patterns in neural precursors and shh-driven medulloblastoma

    doi: 10.1007/s00401-012-0968-6

    Figure Lengend Snippet: Shh-induced mouse medulloblastomas have robust lipogenesis, increased levels of E2F1 and PPARγ and cell proliferation. a Triglyceride accumulation was analyzed in a NeuroD2 -SmoA1 medulloblastoma using Oil Red O staining; neutral lipids appear as red droplets. NeuroD2 -SmoA1 medulloblastoma ( top row ) and adjacent non-tumorous cerebellum ( bottom row ) were subjected to immunofluorescence analyses for E2F1, PPARγ, and the proliferation marker Ki67. Primary and secondary antibodies were used at concentrations of 1:100. Leftmost panel shows hematoxylin and eosin staining (H E) staining of the tumor and cerebellar molecular layer ( mol ) and inner granule layer ( IGL ). The non-tumorous cerebellum immunofluorescence focuses on the boundary between the IGL and molecular layer demonstrated in the H E staining. Magnification: ×1.25 ( leftmost panel ), ×40 ( other images ). Bars 16 μM. Panels demonstrate the typical outcome from 5 different tumor and adjoining cerebellum specimens all paraffin embedded, sectioned, and subjected to immunofluorescence for the aforementioned antibodies. Additionally, the Oil red oil represents a typical outcome based on the staining of frozen sections from 5 different specimens. b Protein lysates were prepared from NeuroD2 -SmoA1 medulloblastoma and normal cerebellum adjacent to the tumor, and then analyzed by western blotting for PPARγ, lipogenic marker (FASN) and proliferation markers (E2F1 and cyclin D2). 40 μg protein/lane was loaded. Data presented are typical for all 5 sets of tumor and cerebellum samples that were studied. c Western blot analysis of proteins regulating proliferation and lipogenesis in adjacent non-tumor cerebellum or medulloblastomas from NeuroD2 -SmoA1 mice treated with DMSO (−) or cdk inhibitor olomoucine (+). The results shown represent a typical outcome amongst the control and experimental groups of mice

    Article Snippet: Animal studies Wild type C57-BL6 mice and NeuroD2-SmoA1 mice bearing tumors were administered 10 % dimethyl sulfoxide (DMSO, control), olomoucine (Calbiochem) at 6 mg/kg daily or GW9662 (Cayman Chemicals) at 10 mg/kg daily by intraperitoneal (i.p.) injection.

    Techniques: Staining, Immunofluorescence, Marker, Western Blot, Mouse Assay

    Shh-induced mouse medulloblastomas have high levels of glycolytic markers of hexokinase II, pyruvate kinase M2, and glucose transporter-4. a NeuroD2 -SmoA1 medulloblastoma ( top row ) and adjacent non-tumor cerebellum ( bottom row ) were subjected to immunofluorescence analyses for glycolysis (HKI, HKII, Glut4, and PKM2). Leftmost panel shows hematoxylin and eosin staining (H E) staining of the tumor and cerebellar molecular layer ( mol ) and inner granule layer ( IGL ). Magnification: ×1.25 ( leftmost panel ), ×40 ( other images ). Bars 16 μM. Results shown are typical for the 5 different tumor and adjoining cerebellum samples used for immunofluorescence in this study. b Protein lysates were prepared from NeuroD2 -SmoA1 medulloblastoma and normal cerebellum adjacent to the tumor, and then analyzed by western blotting for glycolysis. 40 μg protein/lane was loaded. The data shown were repeated with multiple sets of tumor and cerebellum from different NeuroD2 - SmoA1 mice exhibiting tumors. c Western blot analysis of proteins regulating glycolysis in adjacent non-tumor cerebellum or medulloblastomas from NeuroD2 -SmoA1 mice treated with DMSO (−) or cdk inhibitor olomoucine (+). The same protein lysates from Fig. 1 c were blotted for glycolytic markers and this is a typical outcome of those blots

    Journal: Acta Neuropathologica

    Article Title: Hedgehog-mediated regulation of PPAR? controls metabolic patterns in neural precursors and shh-driven medulloblastoma

    doi: 10.1007/s00401-012-0968-6

    Figure Lengend Snippet: Shh-induced mouse medulloblastomas have high levels of glycolytic markers of hexokinase II, pyruvate kinase M2, and glucose transporter-4. a NeuroD2 -SmoA1 medulloblastoma ( top row ) and adjacent non-tumor cerebellum ( bottom row ) were subjected to immunofluorescence analyses for glycolysis (HKI, HKII, Glut4, and PKM2). Leftmost panel shows hematoxylin and eosin staining (H E) staining of the tumor and cerebellar molecular layer ( mol ) and inner granule layer ( IGL ). Magnification: ×1.25 ( leftmost panel ), ×40 ( other images ). Bars 16 μM. Results shown are typical for the 5 different tumor and adjoining cerebellum samples used for immunofluorescence in this study. b Protein lysates were prepared from NeuroD2 -SmoA1 medulloblastoma and normal cerebellum adjacent to the tumor, and then analyzed by western blotting for glycolysis. 40 μg protein/lane was loaded. The data shown were repeated with multiple sets of tumor and cerebellum from different NeuroD2 - SmoA1 mice exhibiting tumors. c Western blot analysis of proteins regulating glycolysis in adjacent non-tumor cerebellum or medulloblastomas from NeuroD2 -SmoA1 mice treated with DMSO (−) or cdk inhibitor olomoucine (+). The same protein lysates from Fig. 1 c were blotted for glycolytic markers and this is a typical outcome of those blots

    Article Snippet: Animal studies Wild type C57-BL6 mice and NeuroD2-SmoA1 mice bearing tumors were administered 10 % dimethyl sulfoxide (DMSO, control), olomoucine (Calbiochem) at 6 mg/kg daily or GW9662 (Cayman Chemicals) at 10 mg/kg daily by intraperitoneal (i.p.) injection.

    Techniques: Immunofluorescence, Staining, Western Blot, Mouse Assay