Journal: bioRxiv

Article Title: Histone succinylation directly inhibits Jumonji domain demethylases and stabilizes repressive chromatin states

doi: 10.64898/2026.05.29.728167

Figure Lengend Snippet: (A) Global histone succinylation measured by LC-MS/MS in HepG2/C3A cells following 48 hours of sodium succinate treatment. (B) Global histone succinylation in 3D cultured HepG2/C3A spheroids following 48 hours and 1 week of sodium succinate treatment. (C) Comparison of the relative abundance of succinyl-lysine residues in H3 and H4, in either treated or untreated, cultured cells (2D) or spheroids (3D). (D) Structure of the nucleosome with H3K64 residues highlighted to demonstrate solvent accessibility. (E) Abundance of succinyl-CoA in the nuclear fraction (left) and non-nuclear fraction (right) following sodium succinate treatment. (F) Total count of HepG2/C3A cells treated with 10 mM sodium succinate continuously for 48 to 144 hours, cell count taken every 48 hours. Data are shown as mean ± SEM; * p-value <0 . 05 in two-tailed Student’s t-test .

Article Snippet: The human hepatocellular carcinoma HepG2/C3A cell line was obtained from the American Type Culture Collection (ATCC, CRL-10741).

Techniques: Liquid Chromatography with Mass Spectroscopy, Cell Culture, Comparison, Solvent, Cell Characterization, Two Tailed Test

Journal: bioRxiv

Article Title: Histone succinylation directly inhibits Jumonji domain demethylases and stabilizes repressive chromatin states

doi: 10.64898/2026.05.29.728167

Figure Lengend Snippet: (A) Schematic of peptide pull-down technique used to identify potential protein interactors with histone succinylation. (B) Volcano plot of fold change and significance of enrichment of nuclear proteins from HepG2/C3A cells to a Ksu synthetic peptide vs. Kac synthetic peptide. (C) Volcano plot of fold change and significance of enrichment of bromodomain proteins to Ksu synthetic histone peptide as compared to the Kac peptide, and (D) Ksu synthetic histone peptide as compared to an unmodified peptide. (E) Volcano plot of fold change and significance of enrichment of JmjC domain demethylases to Ksu synthetic histone peptide as compared to an Kac peptide and (F) Ksu synthetic histone peptide as compared to an unmodified peptide. (G) Crystal structure (PDB 6F6D) of an unmodified histone H3 peptide in the catalytic site of KDM6B. (H) Boltz2 model of a Ksu peptide (AARK(succinyl)A) in the catalytic site of KDM6B. (I) Enrichment of JmjC-domain demethylases to the chromatin-bound proteome of HepG2/C3A cells treated with 10 mM sodium succinate for 48 hours. A -log2 p-value greater than 4 indicates significant enrichment .

Article Snippet: The human hepatocellular carcinoma HepG2/C3A cell line was obtained from the American Type Culture Collection (ATCC, CRL-10741).

Techniques:

Journal: bioRxiv

Article Title: Histone succinylation directly inhibits Jumonji domain demethylases and stabilizes repressive chromatin states

doi: 10.64898/2026.05.29.728167

Figure Lengend Snippet: (A) Known targets of JmjC domain demethylase family enzymes. (B) Relative abundance of methylation on targets of JmjC domain demethylases in HepG2/C3A cells treated with sodium succinate. (C) Relative abundance of methylation on targets of JmjC domain demethylases in HepG2/C3A 3D cells treated with sodium succinate. (D) ChIP-MS shows co-enrichment of succinylated histone peptides with H3K27me3 peptides. Data are shown as mean ± SEM; *p-value <0 . 05, **p-value <0 . 01 in two-tailed Student’s t-test .

Article Snippet: The human hepatocellular carcinoma HepG2/C3A cell line was obtained from the American Type Culture Collection (ATCC, CRL-10741).

Techniques: Methylation, Two Tailed Test

Journal: BioMed Research International

Article Title: In Vitro Antidiabetic Activity and Mechanism of Action of Methanolic Extract of Opuntia stricta Cladodes

doi: 10.1155/bmri/9325356

Figure Lengend Snippet: Cell viability (%). (A) L6 myoblast cells and (B) C3A cells after 24 h of exposure to treatment as indicated.

Article Snippet: The INS‐1 cells ( Rattus norvegicus , male, pancreatic β ‐cell; AddexBio, RRID:CVCL_0351), Caco‐2 cells ( Homo sapiens , male, colon epithelium; American Type Culture Collection [ATCC] HTB‐37, RRID:CVCL_0025), and L6 myoblasts ( Rattus norvegicus , male, skeletal muscle myoblast; RIKEN Cell Bank, RRID:CVCL_0385) were procured from Highveld Biological, South Africa, while the C3A subclone of HepG2 cells ( Homo sapiens , female, hepatocytes, liver tissue; ATCC CRL‐10741, RRID:CVCL_1848) was sourced from the ATCC (Manassas, Virginia, United States).

Techniques:

Journal: BioMed Research International

Article Title: In Vitro Antidiabetic Activity and Mechanism of Action of Methanolic Extract of Opuntia stricta Cladodes

doi: 10.1155/bmri/9325356

Figure Lengend Snippet: Glucose utilization (%) after 24 h of treatment in (A) L6 myoblasts and (B) C3A hepatocytes.

Article Snippet: The INS‐1 cells ( Rattus norvegicus , male, pancreatic β ‐cell; AddexBio, RRID:CVCL_0351), Caco‐2 cells ( Homo sapiens , male, colon epithelium; American Type Culture Collection [ATCC] HTB‐37, RRID:CVCL_0025), and L6 myoblasts ( Rattus norvegicus , male, skeletal muscle myoblast; RIKEN Cell Bank, RRID:CVCL_0385) were procured from Highveld Biological, South Africa, while the C3A subclone of HepG2 cells ( Homo sapiens , female, hepatocytes, liver tissue; ATCC CRL‐10741, RRID:CVCL_1848) was sourced from the ATCC (Manassas, Virginia, United States).

Techniques:

Journal: BioMed Research International

Article Title: In Vitro Antidiabetic Activity and Mechanism of Action of Methanolic Extract of Opuntia stricta Cladodes

doi: 10.1155/bmri/9325356

Figure Lengend Snippet: Glucose uptake (%) after 4 h in (A) L6 myoblasts and (B) C3A hepatocytes following a 24‐h treatment period.

Article Snippet: The INS‐1 cells ( Rattus norvegicus , male, pancreatic β ‐cell; AddexBio, RRID:CVCL_0351), Caco‐2 cells ( Homo sapiens , male, colon epithelium; American Type Culture Collection [ATCC] HTB‐37, RRID:CVCL_0025), and L6 myoblasts ( Rattus norvegicus , male, skeletal muscle myoblast; RIKEN Cell Bank, RRID:CVCL_0385) were procured from Highveld Biological, South Africa, while the C3A subclone of HepG2 cells ( Homo sapiens , female, hepatocytes, liver tissue; ATCC CRL‐10741, RRID:CVCL_1848) was sourced from the ATCC (Manassas, Virginia, United States).

Techniques: