c2c12 cell line  (ATCC)

Journal: Molecular Metabolism

Article Title: Training-induced plasma miR-29a-3p is secreted by skeletal muscle and contributes to metabolic adaptations to resistance exercise in mice

doi: 10.1016/j.molmet.2025.102173

Figure Lengend Snippet: In vitro study of the impact of EPS in miRNA secretion and the modulatory effect of miR-29a-3p inhibition in training-associated miRNAs in mouse myotubes. A . Schematic representation of electrostimulation protocol on C2C12 cells and isolation of miRNAs from EVs and cells. Created with BioRender. B-D. Relative expression levels of miR-30a-5p, let-7f-5p, and miR-29a-3p in cells and EVs at 0, 3, and 20 h after electrostimulation in non-electrostimulated (C, n = 4) and electrostimulated (EPS, n = 4) cells. E-G. Relative expression of cluster 1 miRNAs ( E ), let-7i-5p ( F ), and miR-143-3p ( G ) in C2C12 myotubes where miR-29a-3p expression was inhibited by 70% (miR29dKD, n = 4) and in control myotubes (Control, n = 4). H–I. Relative expression of miR-30a-5p and miR-143-3p in non-electrostimulated (C, n = 4) and electrostimulated (EPS, n = 4) miR-29a-3p dKD cells at 0, 3, and 20 h after electrostimulation. Statistical significance was determined using unpaired, two-tailed Student’s t -test and p -values are indicated in the figures. Data are represented as mean ± SEM. Each dot represents the mean technical duplicate of each cell replicate.

Article Snippet: The C2C12 cell line was cultured in DMEM (Dulbecco’s Modified Eagle Medium, 11965092, Gibco, Thermo Fisher Scientific, Inc., MA, USA).

Techniques: In Vitro, Inhibition, Isolation, Expressing, Control, Two Tailed Test

Journal: Molecular Metabolism

Article Title: Training-induced plasma miR-29a-3p is secreted by skeletal muscle and contributes to metabolic adaptations to resistance exercise in mice

doi: 10.1016/j.molmet.2025.102173

Figure Lengend Snippet: In vitro study of the impact of EPS in miRNA secretion and the modulatory effect of miR-29a-3p inhibition in training-associated miRNAs in mouse myotubes. A . Schematic representation of electrostimulation protocol on C2C12 cells and isolation of miRNAs from EVs and cells. Created with BioRender. B-D. Relative expression levels of miR-30a-5p, let-7f-5p, and miR-29a-3p in cells and EVs at 0, 3, and 20 h after electrostimulation in non-electrostimulated (C, n = 4) and electrostimulated (EPS, n = 4) cells. E-G. Relative expression of cluster 1 miRNAs ( E ), let-7i-5p ( F ), and miR-143-3p ( G ) in C2C12 myotubes where miR-29a-3p expression was inhibited by 70% (miR29dKD, n = 4) and in control myotubes (Control, n = 4). H–I. Relative expression of miR-30a-5p and miR-143-3p in non-electrostimulated (C, n = 4) and electrostimulated (EPS, n = 4) miR-29a-3p dKD cells at 0, 3, and 20 h after electrostimulation. Statistical significance was determined using unpaired, two-tailed Student’s t -test and p -values are indicated in the figures. Data are represented as mean ± SEM. Each dot represents the mean technical duplicate of each cell replicate.

Article Snippet: The mouse skeletal muscle myoblast cell line C2C12 were obtained from the American Type Culture Collection (ATCC CRL-1772).

Techniques: In Vitro, Inhibition, Isolation, Expressing, Control, Two Tailed Test

Journal: FASEB BioAdvances

Article Title: Irisin Prevents the Effects of Simulated Microgravity on Bone and Muscle Differentiation Markers

doi: 10.1096/fba.2025-00085

Figure Lengend Snippet: Representative images of myoblasts (C2C12), under different culture conditions. (a) myoblasts cultured in ground control conditions; (b) myoblasts exposed to simulated microgravity for 5 days; (c) myoblasts exposed to simulated microgravity for 5 days and treated with irisin during the last 8 h of RPM exposure (irisin‐RPM).

Article Snippet: The cell line C2C12 ( Mus Musculus ) was purchased by ATCC (ATCC, Manassas, VA, USA) and amplified up to passage P3.

Techniques: Cell Culture, Control

Journal: FASEB BioAdvances

Article Title: Irisin Prevents the Effects of Simulated Microgravity on Bone and Muscle Differentiation Markers

doi: 10.1096/fba.2025-00085

Figure Lengend Snippet: qPCR shows mRNA expression levels of Pax7 , Myf5 and MyoD in myoblasts (C2C12) cells. Data indicate that irisin treatment prevents the increase of the expression of Pax7 (a) and MyoD (c) and brings the expression to values of control condition, while a partial recovery is observed in Myf5 (b) expression. Ctrl, control condition; RPM, random position machine.

Article Snippet: The cell line C2C12 ( Mus Musculus ) was purchased by ATCC (ATCC, Manassas, VA, USA) and amplified up to passage P3.

Techniques: Expressing, Control

Journal: FASEB BioAdvances

Article Title: Irisin Prevents the Effects of Simulated Microgravity on Bone and Muscle Differentiation Markers

doi: 10.1096/fba.2025-00085

Figure Lengend Snippet: qPCR shows mRNA expression levels of Adam10 and Fndc5 in myoblasts (C2C12) cells. (a) irisin treatment increases the gene expression of Adam10 in RPM condition compared with untreated RPM cell cultures and Ctrl cell cultures. (b) irisin treatment increases the gene expression of Fndc5 in RPM condition compared with control condition. Ctrl, control condition; RPM, random position machine.

Article Snippet: The cell line C2C12 ( Mus Musculus ) was purchased by ATCC (ATCC, Manassas, VA, USA) and amplified up to passage P3.

Techniques: Expressing, Gene Expression, Control

Journal: bioRxiv

Article Title: Aqueous two-phase bioinks for discrete packing and compartmentalisation of 3D bioprinted cells

doi: 10.1101/2025.06.27.661968

Figure Lengend Snippet: Cell viability of C2C12 in ATPS systems. a-i) Confocal images with C2C12 labelled with Calcein to show live cells (green) and cells labelled with propidium iodide to show dead cells (red). Quantification of cell viability in ATPS systems where a-ii) GelMA was cross-linked and in samples where a-iii) GelMA was removed. Scale bars: (a) 100 µm. Statistical significances were assessed by two-way ANOVA. Mean ± S.D. n=3, *p<0.05.

Article Snippet: The A549 human lung carcinoma epithelial-like cell line, C2C12 mouse pre-myoblast cell line and MG63 human osteosarcoma cell line were obtained from ATCC (USA).

Techniques:

Journal: bioRxiv

Article Title: Aqueous two-phase bioinks for discrete packing and compartmentalisation of 3D bioprinted cells

doi: 10.1101/2025.06.27.661968

Figure Lengend Snippet: Spatial distribution of C2C12 in ATPS systems. a-b-c) Confocal images with C2C12 labelled with DAPI to show nuclei (blue) and cells marked with phalloidin to show f-actin (magenta). d) Orthogonal view of image c-i. Circularity analysis conducted on the formulation e-i) NaCl 0 g/L with GelMA, e-ii) NaCl 9 g/L with GelMA, e-iii) NaCl 36 g/L with GelMA, f-i) NaCl 0 g/L without GelMA, f-ii) NaCl 9 g/L without GelMA and f-iii) NaCl 36 g/L without GelMA. g) Cell circularity as a function of salt concentration. g-i) Cell circularity for each NaCl composition within the ATPS samples is shown. The pink contour marks samples with + GelMA; the blue contour marks samples with - GelMA. g-ii) Cell circularity is inversely proportional to the salt concentration in the hydrogels. When the amount of NaCl in the scaffolds is higher, the cells are more elongated. Scale bars: (c) 100 µm. Statistical significances were assessed by two-way ANOVA. Mean ± S.D. n=3, ****p<0.0001, ***p<0.001, **p<0.01, *p<0.05.

Article Snippet: The A549 human lung carcinoma epithelial-like cell line, C2C12 mouse pre-myoblast cell line and MG63 human osteosarcoma cell line were obtained from ATCC (USA).

Techniques: Formulation, Concentration Assay

Journal: bioRxiv

Article Title: Aqueous two-phase bioinks for discrete packing and compartmentalisation of 3D bioprinted cells

doi: 10.1101/2025.06.27.661968

Figure Lengend Snippet: Partitioning of different cell types within the various ATPS scaffold formulations. Brightfield and confocal (merged) images representative of cells labelled with DAPI to show the nucleus (blue) of the different cell types (A549, C2C12, MG63, HBMSCs), and GelMA marked with rhodamine B to show the inner phase (red) of the scaffolds at 0 - 9 - 36 g/L. In all cell types, images were taken both under conditions where GelMA was not chemically cross-linked (-GelMA) and under conditions where GelMA was chemically cross-linked (+GelMA). Scale bars: (a, b, c, d) 100 μm. Mean ± S.D. n=3.

Article Snippet: The A549 human lung carcinoma epithelial-like cell line, C2C12 mouse pre-myoblast cell line and MG63 human osteosarcoma cell line were obtained from ATCC (USA).

Techniques: