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Waters Corporation c18 seppak
C18 Seppak, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c18 seppak/product/Waters Corporation
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
c18 seppak - by Bioz Stars, 2020-04
95/100 stars

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Centrifugation:

Article Title: Induction of virulence factors in Giardia duodenalis independent of host attachment
Article Snippet: Each of the 10 samples were then combined, dried down using a vacuum centrifuge, reconstituted in 1% formic acid, and desalted on a 200 mg C18 SepPak (Waters, Massachusetts). .. A total of 36 fractions of varying volumes were collected and dried down by vacuum centrifugation, before being combined to 12 fractions based on peptide content.

Article Title: Spectral library searching to identify cross-linked peptides
Article Snippet: After digestion, peptide lysates were acidified by addition of 0.3% trifluoracetic acid and cleared by centrifugation. .. Peptides were desalted (manufacturers protocol for C18-SepPak, Waters) to be fractionated by SCX chromatography on a Phenomenex Luna SCX column mounted on an Agilent 1200 HPLC from 100% buffer A (30% ACN, 7mM KH2 PO4 , pH 2.5) to 30% buffer B (30% ACN, 7mM KH2 PO4 , 350mM KCl, pH 2.5).

Synthesized:

Article Title: Modeling Considerations for 11C-CUMI-101, an Agonist Radiotracer for Imaging Serotonin 1A Receptor In Vivo with PET
Article Snippet: 11 C-CUMI-101 was synthesized as described by our laboratory ( ). .. The final product was purified by high-performance liquid chromatography (HPLC) and a C18 SepPak (Waters).

Activity Assay:

Article Title: Modeling Considerations for 11C-CUMI-101, an Agonist Radiotracer for Imaging Serotonin 1A Receptor In Vivo with PET
Article Snippet: The final product was purified by high-performance liquid chromatography (HPLC) and a C18 SepPak (Waters). .. A small portion of the product was analyzed with analytic HPLC for chemical and radiochemical purities, specific activity, and other quality control indices.

Mass Spectrometry:

Article Title: In vitro synthesis of the nucleotide loop of cobalamin by Salmonella typhimurium enzymes
Article Snippet: Paragraph title: Mass Spectrometry. ... The HPLC-purified product of the NLA reactions was desalted by adsorbing it onto a C18 SepPak (Waters), washing it with water to remove salt, eluting it with 100% (vol/vol) methanol, and vacuuming it to dryness.

Article Title: Parasitoid Jewel Wasp Mounts Multipronged Neurochemical Attack to Hijack a Host Brain
Article Snippet: All venom samples were desalted using C18 Zip Tip (Millipore Corp., Bedford, MA) or C18 SepPak (Waters, Milford, MA) cartridges, dried and resuspended in 0.1% formic acid. .. Two trypsinized samples and two samples without protease treatment were analyzed at the Smoler Proteomics Center at the Technion Israel Institute of Technology via reverse-phase liquid chromatography on 0.075 × 250-mm fused silica capillaries (J & W Scientific/Agilent, Folsom, CA) packed with Reprosil reversed-phase material and analyzed on a Q-Exactive plus mass spectrometer (Thermo Fisher Scientific, Waltham, MA) in positive mode using repetitively full MS scan followed by high collision dissociation of the 10 most dominant ions selected from the first MS scan.

Article Title: The Tarantula Toxin Psalmotoxin 1 Inhibits Acid-sensing Ion Channel (ASIC) 1a by Increasing Its Apparent H+ Affinity
Article Snippet: The solution was concentrated using a C18 SepPak (Waters) cartridge and finally lyophilized. .. The quality of the product was confirmed by analytical HPLC, matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-MS), and electrospray ionization mass spectrometry (ESI-MS), giving the correct mass of oxidized product.

Article Title: Characterizing the O-glycosylation landscape of human plasma, platelets, and endothelial cells
Article Snippet: 0.5 mL of packed cells or 2 mL plasma was prepared for O -glycoproteomic analysis as previously described., Briefly, samples were sonicated, de-sialylated with 0.1 U/mL Clostridium perfringens neuraminidase Type VI (Sigma) for 1 hour at 37°C, reduced (5 mM dithiothreitol, 60°C, 30 minutes), alkylated (10 mM iodoacetamide, room temperature, 30 minutes), and subjected to overnight digestion with MS-grade trypsin (Roche) or chymotrypsin (Roche) at 1:50 wt/wt. .. Samples were then purified on a C18 SepPak (Waters) and dried by SpeedVac.

BIA-KA:

Article Title: Exoproteome of Staphylococcus aureus reveals putative determinants of nasal carriage
Article Snippet: The extract was further purified by a C18 SepPak (Waters Corporation, MA, USA) column according to the manufacturer’s instructions, except that the final elution was in 80% acetonitrile/ 20% HPLC grade water. .. The total protein concentration of the sample was estimated using Micro BCA™ Protein Assay Kit (Thermo Scientific, Pierce, IL, USA) according to the manufacturer’s instructions.

Article Title: Induction of virulence factors in Giardia duodenalis independent of host attachment
Article Snippet: Samples were acidified with trifluoroacetic acid, and then desalted on a 200 mg C18 SepPak (Waters, Massachusetts). .. Samples were acidified with trifluoroacetic acid, and then desalted on a 200 mg C18 SepPak (Waters, Massachusetts).

Modification:

Article Title: Exoproteome of Staphylococcus aureus reveals putative determinants of nasal carriage
Article Snippet: Based on the growth kinetics of our SA strains, a modified approach from Dreisbach and colleagues was utilized for SA exoproteome preparation. .. The extract was further purified by a C18 SepPak (Waters Corporation, MA, USA) column according to the manufacturer’s instructions, except that the final elution was in 80% acetonitrile/ 20% HPLC grade water.

High Performance Liquid Chromatography:

Article Title: In vitro synthesis of the nucleotide loop of cobalamin by Salmonella typhimurium enzymes
Article Snippet: .. The HPLC-purified product of the NLA reactions was desalted by adsorbing it onto a C18 SepPak (Waters), washing it with water to remove salt, eluting it with 100% (vol/vol) methanol, and vacuuming it to dryness. .. The sample was submitted for analysis to the Mass Spectrometry Facility at the University of Wisconsin-Madison Biotechnology Center.

Article Title: Exoproteome of Staphylococcus aureus reveals putative determinants of nasal carriage
Article Snippet: .. The extract was further purified by a C18 SepPak (Waters Corporation, MA, USA) column according to the manufacturer’s instructions, except that the final elution was in 80% acetonitrile/ 20% HPLC grade water. .. The sample was dehydrated to 10μl and diluted with 100μl of HPLC grade water.

Article Title: Modeling Considerations for 11C-CUMI-101, an Agonist Radiotracer for Imaging Serotonin 1A Receptor In Vivo with PET
Article Snippet: .. The final product was purified by high-performance liquid chromatography (HPLC) and a C18 SepPak (Waters). ..

Article Title: N-Glycomic and -Glycoproteomic Studies in the Social Amoebae
Article Snippet: Acetonitrile (ACN) (HPLC grade), dried over 3-Å molecular sieves (Sigma-Aldrich) ( see ). .. C18 -SepPak (100 mg) cartridge (Waters).

Article Title: Induction of virulence factors in Giardia duodenalis independent of host attachment
Article Snippet: Each of the 10 samples were then combined, dried down using a vacuum centrifuge, reconstituted in 1% formic acid, and desalted on a 200 mg C18 SepPak (Waters, Massachusetts). .. The combined sample of TMT labelled peptides were dried down again and reconstituted in 1% formic acid prior to fractionation by strong cation exchange (SCX) high pressure liquid chromotography (HPLC) using a PolyLC PolySulfoethyl A (200 mm × 2.1 mm × 5 μm, 200 Å) column and UV detection at 210 nm.

Article Title: Spectral library searching to identify cross-linked peptides
Article Snippet: .. Peptides were desalted (manufacturers protocol for C18-SepPak, Waters) to be fractionated by SCX chromatography on a Phenomenex Luna SCX column mounted on an Agilent 1200 HPLC from 100% buffer A (30% ACN, 7mM KH2 PO4 , pH 2.5) to 30% buffer B (30% ACN, 7mM KH2 PO4 , 350mM KCl, pH 2.5). .. SCX fractions were subsequently enriched for biotin containing cross-linked peptide-peptide relationships with monomeric avidin beads (UltraLink, Pierce) and eluted with 70% ACN, 1% trifluoroacetic acid.

Article Title: Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases
Article Snippet: Oligonucleotides were desalted by loading onto on a C18 SepPak (Waters) which had been pre-equilibrated by washing with acetonitrile, H2 O, and 10 mM NH4 OAc. .. Cy5-labeled oligonucleotides which were purchased HPLC and were not gel purified.

Article Title: The Tarantula Toxin Psalmotoxin 1 Inhibits Acid-sensing Ion Channel (ASIC) 1a by Increasing Its Apparent H+ Affinity
Article Snippet: The solution was adjusted to pH 8.0 with aqueous NH4 OH and stirred slowly at 4°C for 3 d. The folding reaction was monitored by analytical HPLC. .. The solution was concentrated using a C18 SepPak (Waters) cartridge and finally lyophilized.

Flow Cytometry:

Article Title: Induction of virulence factors in Giardia duodenalis independent of host attachment
Article Snippet: Each of the 10 samples were then combined, dried down using a vacuum centrifuge, reconstituted in 1% formic acid, and desalted on a 200 mg C18 SepPak (Waters, Massachusetts). .. Samples were resuspended and initially loaded with buffer A (5 mM KH2 PO4 , pH 2.7, 25% ACN), and fractionated with a linear gradient of 10–45% buffer B (5 mM KH2 PO4 , pH 2.72, 350 mM KCl, 25% ACN) for 70 minutes, which was rapidly increased from 45–100% buffer B for 10 minutes at a flow rate of 300 μl/min.

Chromatography:

Article Title: Spectral library searching to identify cross-linked peptides
Article Snippet: .. Peptides were desalted (manufacturers protocol for C18-SepPak, Waters) to be fractionated by SCX chromatography on a Phenomenex Luna SCX column mounted on an Agilent 1200 HPLC from 100% buffer A (30% ACN, 7mM KH2 PO4 , pH 2.5) to 30% buffer B (30% ACN, 7mM KH2 PO4 , 350mM KCl, pH 2.5). .. SCX fractions were subsequently enriched for biotin containing cross-linked peptide-peptide relationships with monomeric avidin beads (UltraLink, Pierce) and eluted with 70% ACN, 1% trifluoroacetic acid.

Article Title: The Tarantula Toxin Psalmotoxin 1 Inhibits Acid-sensing Ion Channel (ASIC) 1a by Increasing Its Apparent H+ Affinity
Article Snippet: The solution was concentrated using a C18 SepPak (Waters) cartridge and finally lyophilized. .. Purification of the oxidized product was achieved first by chromatography on a C8 column using the system above and yielding a purity of ∼90%.

Article Title: Characterizing the O-glycosylation landscape of human plasma, platelets, and endothelial cells
Article Snippet: Samples were then purified on a C18 SepPak (Waters) and dried by SpeedVac. .. Glycopeptides were enriched by lectin chromatography using PNA- or VVA-agarose (VectorLabs) on an AKTA fast protein liquid chromatography system.

Buffer Exchange:

Article Title: Spectral library searching to identify cross-linked peptides
Article Snippet: Cells were lysed by cryo-milling (3x 30Hz, 1 min) with either 8M urea ( S. cerevisiae and A. baumannii ) or with 4% SDS buffered with 100mM Tris-Cl pH 8.0, 150mM NaCl with subsequent detergent removal by buffer exchange. .. Peptides were desalted (manufacturers protocol for C18-SepPak, Waters) to be fractionated by SCX chromatography on a Phenomenex Luna SCX column mounted on an Agilent 1200 HPLC from 100% buffer A (30% ACN, 7mM KH2 PO4 , pH 2.5) to 30% buffer B (30% ACN, 7mM KH2 PO4 , 350mM KCl, pH 2.5).

Cell Culture:

Article Title: Spectral library searching to identify cross-linked peptides
Article Snippet: Paragraph title: Cell Culture, Cell Lysis and Sample Preparation for LC-MS/MS ... Peptides were desalted (manufacturers protocol for C18-SepPak, Waters) to be fractionated by SCX chromatography on a Phenomenex Luna SCX column mounted on an Agilent 1200 HPLC from 100% buffer A (30% ACN, 7mM KH2 PO4 , pH 2.5) to 30% buffer B (30% ACN, 7mM KH2 PO4 , 350mM KCl, pH 2.5).

Protein Concentration:

Article Title: Exoproteome of Staphylococcus aureus reveals putative determinants of nasal carriage
Article Snippet: The extract was further purified by a C18 SepPak (Waters Corporation, MA, USA) column according to the manufacturer’s instructions, except that the final elution was in 80% acetonitrile/ 20% HPLC grade water. .. The total protein concentration of the sample was estimated using Micro BCA™ Protein Assay Kit (Thermo Scientific, Pierce, IL, USA) according to the manufacturer’s instructions.

Sonication:

Article Title: Characterizing the O-glycosylation landscape of human plasma, platelets, and endothelial cells
Article Snippet: 0.5 mL of packed cells or 2 mL plasma was prepared for O -glycoproteomic analysis as previously described., Briefly, samples were sonicated, de-sialylated with 0.1 U/mL Clostridium perfringens neuraminidase Type VI (Sigma) for 1 hour at 37°C, reduced (5 mM dithiothreitol, 60°C, 30 minutes), alkylated (10 mM iodoacetamide, room temperature, 30 minutes), and subjected to overnight digestion with MS-grade trypsin (Roche) or chymotrypsin (Roche) at 1:50 wt/wt. .. Samples were then purified on a C18 SepPak (Waters) and dried by SpeedVac.

Molecular Weight:

Article Title: Spectral library searching to identify cross-linked peptides
Article Snippet: SDS lysates were buffer exchanged to 8M urea buffer using the max volume of 30kDa molecular weight cutoff spin filters (15mL capacity, Millipore) until no detectable SDS was present. .. Peptides were desalted (manufacturers protocol for C18-SepPak, Waters) to be fractionated by SCX chromatography on a Phenomenex Luna SCX column mounted on an Agilent 1200 HPLC from 100% buffer A (30% ACN, 7mM KH2 PO4 , pH 2.5) to 30% buffer B (30% ACN, 7mM KH2 PO4 , 350mM KCl, pH 2.5).

Tandem Mass Spectroscopy:

Article Title: Characterizing the O-glycosylation landscape of human plasma, platelets, and endothelial cells
Article Snippet: Samples were then purified on a C18 SepPak (Waters) and dried by SpeedVac. .. MS/MS spectra were interrogated against the nonredundant human proteome using the SEQUEST-HT search engine in Proteome Discoverer.

Avidin-Biotin Assay:

Article Title: Spectral library searching to identify cross-linked peptides
Article Snippet: Peptides were desalted (manufacturers protocol for C18-SepPak, Waters) to be fractionated by SCX chromatography on a Phenomenex Luna SCX column mounted on an Agilent 1200 HPLC from 100% buffer A (30% ACN, 7mM KH2 PO4 , pH 2.5) to 30% buffer B (30% ACN, 7mM KH2 PO4 , 350mM KCl, pH 2.5). .. SCX fractions were subsequently enriched for biotin containing cross-linked peptide-peptide relationships with monomeric avidin beads (UltraLink, Pierce) and eluted with 70% ACN, 1% trifluoroacetic acid.

Purification:

Article Title: Exoproteome of Staphylococcus aureus reveals putative determinants of nasal carriage
Article Snippet: .. The extract was further purified by a C18 SepPak (Waters Corporation, MA, USA) column according to the manufacturer’s instructions, except that the final elution was in 80% acetonitrile/ 20% HPLC grade water. .. The sample was dehydrated to 10μl and diluted with 100μl of HPLC grade water.

Article Title: Modeling Considerations for 11C-CUMI-101, an Agonist Radiotracer for Imaging Serotonin 1A Receptor In Vivo with PET
Article Snippet: .. The final product was purified by high-performance liquid chromatography (HPLC) and a C18 SepPak (Waters). ..

Article Title: Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases
Article Snippet: Oligonucleotides for assays Oligonucleotides listed in Supplementary Table were purchased from Integrated DNA Technologies and gel purified by denaturing 20% PAGE, visualized by UV shadowing to excise the band, and electroeluted from the acrylamide using an Elutrap (Whatman). .. Oligonucleotides were desalted by loading onto on a C18 SepPak (Waters) which had been pre-equilibrated by washing with acetonitrile, H2 O, and 10 mM NH4 OAc.

Article Title: The Tarantula Toxin Psalmotoxin 1 Inhibits Acid-sensing Ion Channel (ASIC) 1a by Increasing Its Apparent H+ Affinity
Article Snippet: Oxidation of the reduced toxin was achieved by dissolving the purified peptide with 2 M acetic acid, and diluted to a peptide concentration of 0.015 mM in the presence of reduced/oxidized glutathione (molar ratio of peptide/GSH/GSSG was 1:100:10) and 2 M guanidine hydrochloride. .. The solution was concentrated using a C18 SepPak (Waters) cartridge and finally lyophilized.

Article Title: Characterizing the O-glycosylation landscape of human plasma, platelets, and endothelial cells
Article Snippet: .. Samples were then purified on a C18 SepPak (Waters) and dried by SpeedVac. .. Glycopeptides were enriched by lectin chromatography using PNA- or VVA-agarose (VectorLabs) on an AKTA fast protein liquid chromatography system.

Protein Extraction:

Article Title: Induction of virulence factors in Giardia duodenalis independent of host attachment
Article Snippet: Paragraph title: Protein extraction and digestion ... Samples were acidified with trifluoroacetic acid, and then desalted on a 200 mg C18 SepPak (Waters, Massachusetts).

Fast Protein Liquid Chromatography:

Article Title: Characterizing the O-glycosylation landscape of human plasma, platelets, and endothelial cells
Article Snippet: Samples were then purified on a C18 SepPak (Waters) and dried by SpeedVac. .. Glycopeptides were enriched by lectin chromatography using PNA- or VVA-agarose (VectorLabs) on an AKTA fast protein liquid chromatography system.

Polyacrylamide Gel Electrophoresis:

Article Title: Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases
Article Snippet: Oligonucleotides for assays Oligonucleotides listed in Supplementary Table were purchased from Integrated DNA Technologies and gel purified by denaturing 20% PAGE, visualized by UV shadowing to excise the band, and electroeluted from the acrylamide using an Elutrap (Whatman). .. Oligonucleotides were desalted by loading onto on a C18 SepPak (Waters) which had been pre-equilibrated by washing with acetonitrile, H2 O, and 10 mM NH4 OAc.

Liquid Chromatography:

Article Title: Parasitoid Jewel Wasp Mounts Multipronged Neurochemical Attack to Hijack a Host Brain
Article Snippet: All venom samples were desalted using C18 Zip Tip (Millipore Corp., Bedford, MA) or C18 SepPak (Waters, Milford, MA) cartridges, dried and resuspended in 0.1% formic acid. .. Two trypsinized samples and two samples without protease treatment were analyzed at the Smoler Proteomics Center at the Technion Israel Institute of Technology via reverse-phase liquid chromatography on 0.075 × 250-mm fused silica capillaries (J & W Scientific/Agilent, Folsom, CA) packed with Reprosil reversed-phase material and analyzed on a Q-Exactive plus mass spectrometer (Thermo Fisher Scientific, Waltham, MA) in positive mode using repetitively full MS scan followed by high collision dissociation of the 10 most dominant ions selected from the first MS scan.

Sample Prep:

Article Title: Spectral library searching to identify cross-linked peptides
Article Snippet: Paragraph title: Cell Culture, Cell Lysis and Sample Preparation for LC-MS/MS ... Peptides were desalted (manufacturers protocol for C18-SepPak, Waters) to be fractionated by SCX chromatography on a Phenomenex Luna SCX column mounted on an Agilent 1200 HPLC from 100% buffer A (30% ACN, 7mM KH2 PO4 , pH 2.5) to 30% buffer B (30% ACN, 7mM KH2 PO4 , 350mM KCl, pH 2.5).

Concentration Assay:

Article Title: Induction of virulence factors in Giardia duodenalis independent of host attachment
Article Snippet: Samples were acidified with trifluoroacetic acid, and then desalted on a 200 mg C18 SepPak (Waters, Massachusetts). .. Samples were acidified with trifluoroacetic acid, and then desalted on a 200 mg C18 SepPak (Waters, Massachusetts).

Article Title: The Tarantula Toxin Psalmotoxin 1 Inhibits Acid-sensing Ion Channel (ASIC) 1a by Increasing Its Apparent H+ Affinity
Article Snippet: Oxidation of the reduced toxin was achieved by dissolving the purified peptide with 2 M acetic acid, and diluted to a peptide concentration of 0.015 mM in the presence of reduced/oxidized glutathione (molar ratio of peptide/GSH/GSSG was 1:100:10) and 2 M guanidine hydrochloride. .. The solution was concentrated using a C18 SepPak (Waters) cartridge and finally lyophilized.

Fractionation:

Article Title: Induction of virulence factors in Giardia duodenalis independent of host attachment
Article Snippet: Samples were acidified with trifluoroacetic acid, and then desalted on a 200 mg C18 SepPak (Waters, Massachusetts). .. Samples were acidified with trifluoroacetic acid, and then desalted on a 200 mg C18 SepPak (Waters, Massachusetts).

Article Title: Induction of virulence factors in Giardia duodenalis independent of host attachment
Article Snippet: Each of the 10 samples were then combined, dried down using a vacuum centrifuge, reconstituted in 1% formic acid, and desalted on a 200 mg C18 SepPak (Waters, Massachusetts). .. The combined sample of TMT labelled peptides were dried down again and reconstituted in 1% formic acid prior to fractionation by strong cation exchange (SCX) high pressure liquid chromotography (HPLC) using a PolyLC PolySulfoethyl A (200 mm × 2.1 mm × 5 μm, 200 Å) column and UV detection at 210 nm.

Lysis:

Article Title: Spectral library searching to identify cross-linked peptides
Article Snippet: Paragraph title: Cell Culture, Cell Lysis and Sample Preparation for LC-MS/MS ... Peptides were desalted (manufacturers protocol for C18-SepPak, Waters) to be fractionated by SCX chromatography on a Phenomenex Luna SCX column mounted on an Agilent 1200 HPLC from 100% buffer A (30% ACN, 7mM KH2 PO4 , pH 2.5) to 30% buffer B (30% ACN, 7mM KH2 PO4 , 350mM KCl, pH 2.5).

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  • 94
    Waters Corporation spe c18 column
    Accumulation of stress-related hormones during drought. WT (white bars) and transgenic line S-7 (black bars) were subjected to 14-day drought as described in Fig 2 . The level of (A) absicisic acid ABA; (B) sum of active cytokinins, CK; (C) salicylic acid, SA were determined at D0, D6, D14 and RW1. Samples (0.5g of fresh leaf tissue without the midrib) were collected from the first fully developed, undamaged leaf from the top of plant at 4 hours after turning the light. Labeled internal standards were added to the leaf samples before homogenization. Hormones were then extracted, purified using a <t>SPE-C18</t> column and separated on a reverse phase-cation exchange SPE column. Hormones were quantified using a hybrid triple quadruple/linear ion trap mass spectrometer. The level of ABA and SA is expressed as nmol g -1 of fresh weight; the levels of cytokinins–as pmol g -1 of fresh weight. Results are means ±SE (n = 3). Homogenic groups are determined by Tukey HSD (Honestly Significant Differences) test. The same letters designate values belong to the same homogenic group (p
    Spe C18 Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spe c18 column/product/Waters Corporation
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    spe c18 column - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    94
    Waters Corporation c18 seppak purified rgp
    Holothuria scabra oocyte maturation bioassay with recombinant <t>RGP.</t> Ovarian segments were incubated for 3 h in (A) crude supernatant, CS; (B) crude concentrated RGP, CR; (C) <t>SepPak-purified</t> RGP, SR; inset shows higher magnification of mature oocytes from ovarian segment incubated in 96 μg/μL SR; (D) His and Amicon-purified RGP, HR; (E) yeast solution without RGP, YS; (F) filtered artificial sea water, FASW; and (G) radial nerve extract, RNE. Two insets (red boxes) indicate higher magnification of mature oocytes inside ovarian segment incubated in SR (96 μg/μL) and RNE after 3 h. DO indicates the distorted oocyte; GV, germinal vesicle; GVBD, germinal vesicle breakdown. All scale bars indicate 200 μm.
    C18 Seppak Purified Rgp, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 seppak purified rgp/product/Waters Corporation
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c18 seppak purified rgp - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    94
    Waters Corporation cartridge
    Holothuria scabra oocyte maturation bioassay with recombinant <t>RGP.</t> Ovarian segments were incubated for 3 h in (A) crude supernatant, CS; (B) crude concentrated RGP, CR; (C) <t>SepPak-purified</t> RGP, SR; inset shows higher magnification of mature oocytes from ovarian segment incubated in 96 μg/μL SR; (D) His and Amicon-purified RGP, HR; (E) yeast solution without RGP, YS; (F) filtered artificial sea water, FASW; and (G) radial nerve extract, RNE. Two insets (red boxes) indicate higher magnification of mature oocytes inside ovarian segment incubated in SR (96 μg/μL) and RNE after 3 h. DO indicates the distorted oocyte; GV, germinal vesicle; GVBD, germinal vesicle breakdown. All scale bars indicate 200 μm.
    Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cartridge/product/Waters Corporation
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cartridge - by Bioz Stars, 2020-04
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    98
    Waters Corporation solid phase extraction
    Holothuria scabra oocyte maturation bioassay with recombinant <t>RGP.</t> Ovarian segments were incubated for 3 h in (A) crude supernatant, CS; (B) crude concentrated RGP, CR; (C) <t>SepPak-purified</t> RGP, SR; inset shows higher magnification of mature oocytes from ovarian segment incubated in 96 μg/μL SR; (D) His and Amicon-purified RGP, HR; (E) yeast solution without RGP, YS; (F) filtered artificial sea water, FASW; and (G) radial nerve extract, RNE. Two insets (red boxes) indicate higher magnification of mature oocytes inside ovarian segment incubated in SR (96 μg/μL) and RNE after 3 h. DO indicates the distorted oocyte; GV, germinal vesicle; GVBD, germinal vesicle breakdown. All scale bars indicate 200 μm.
    Solid Phase Extraction, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 98/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solid phase extraction/product/Waters Corporation
    Average 98 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    solid phase extraction - by Bioz Stars, 2020-04
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    Image Search Results


    Accumulation of stress-related hormones during drought. WT (white bars) and transgenic line S-7 (black bars) were subjected to 14-day drought as described in Fig 2 . The level of (A) absicisic acid ABA; (B) sum of active cytokinins, CK; (C) salicylic acid, SA were determined at D0, D6, D14 and RW1. Samples (0.5g of fresh leaf tissue without the midrib) were collected from the first fully developed, undamaged leaf from the top of plant at 4 hours after turning the light. Labeled internal standards were added to the leaf samples before homogenization. Hormones were then extracted, purified using a SPE-C18 column and separated on a reverse phase-cation exchange SPE column. Hormones were quantified using a hybrid triple quadruple/linear ion trap mass spectrometer. The level of ABA and SA is expressed as nmol g -1 of fresh weight; the levels of cytokinins–as pmol g -1 of fresh weight. Results are means ±SE (n = 3). Homogenic groups are determined by Tukey HSD (Honestly Significant Differences) test. The same letters designate values belong to the same homogenic group (p

    Journal: PLoS ONE

    Article Title: Potato Annexin STANN1 Promotes Drought Tolerance and Mitigates Light Stress in Transgenic Solanum tuberosum L. Plants

    doi: 10.1371/journal.pone.0132683

    Figure Lengend Snippet: Accumulation of stress-related hormones during drought. WT (white bars) and transgenic line S-7 (black bars) were subjected to 14-day drought as described in Fig 2 . The level of (A) absicisic acid ABA; (B) sum of active cytokinins, CK; (C) salicylic acid, SA were determined at D0, D6, D14 and RW1. Samples (0.5g of fresh leaf tissue without the midrib) were collected from the first fully developed, undamaged leaf from the top of plant at 4 hours after turning the light. Labeled internal standards were added to the leaf samples before homogenization. Hormones were then extracted, purified using a SPE-C18 column and separated on a reverse phase-cation exchange SPE column. Hormones were quantified using a hybrid triple quadruple/linear ion trap mass spectrometer. The level of ABA and SA is expressed as nmol g -1 of fresh weight; the levels of cytokinins–as pmol g -1 of fresh weight. Results are means ±SE (n = 3). Homogenic groups are determined by Tukey HSD (Honestly Significant Differences) test. The same letters designate values belong to the same homogenic group (p

    Article Snippet: Extracts were purified using a SPE-C18 column (SepPak-C18, Waters), and separated on a reverse phase-cation exchange SPE column (Oasis-MCX, Waters).

    Techniques: Transgenic Assay, Labeling, Homogenization, Purification, Mass Spectrometry

    Holothuria scabra oocyte maturation bioassay with recombinant RGP. Ovarian segments were incubated for 3 h in (A) crude supernatant, CS; (B) crude concentrated RGP, CR; (C) SepPak-purified RGP, SR; inset shows higher magnification of mature oocytes from ovarian segment incubated in 96 μg/μL SR; (D) His and Amicon-purified RGP, HR; (E) yeast solution without RGP, YS; (F) filtered artificial sea water, FASW; and (G) radial nerve extract, RNE. Two insets (red boxes) indicate higher magnification of mature oocytes inside ovarian segment incubated in SR (96 μg/μL) and RNE after 3 h. DO indicates the distorted oocyte; GV, germinal vesicle; GVBD, germinal vesicle breakdown. All scale bars indicate 200 μm.

    Journal: Frontiers in Genetics

    Article Title: Aquaculture Breeding Enhancement: Maturation and Spawning in Sea Cucumbers Using a Recombinant Relaxin-Like Gonad-Stimulating Peptide

    doi: 10.3389/fgene.2019.00077

    Figure Lengend Snippet: Holothuria scabra oocyte maturation bioassay with recombinant RGP. Ovarian segments were incubated for 3 h in (A) crude supernatant, CS; (B) crude concentrated RGP, CR; (C) SepPak-purified RGP, SR; inset shows higher magnification of mature oocytes from ovarian segment incubated in 96 μg/μL SR; (D) His and Amicon-purified RGP, HR; (E) yeast solution without RGP, YS; (F) filtered artificial sea water, FASW; and (G) radial nerve extract, RNE. Two insets (red boxes) indicate higher magnification of mature oocytes inside ovarian segment incubated in SR (96 μg/μL) and RNE after 3 h. DO indicates the distorted oocyte; GV, germinal vesicle; GVBD, germinal vesicle breakdown. All scale bars indicate 200 μm.

    Article Snippet: C18 SepPak-purified RGP (SR): CS was purified through C18 SepPak cartridge (Waters Corporation) following the manufacturer's instruction.

    Techniques: Recombinant, Incubation, Purification