Structured Review

Waters Corporation c18 sep pak cartridge
Representative radiochromatograms of [ 99m Tc(CO) 3 - 1 ] 3+ (A) and 99m Tc-HYNIC- 1 (B) before <t>C18</t> <t>Sep-Pak</t> purification.
C18 Sep Pak Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c18 sep pak cartridge/product/Waters Corporation
Average 93 stars, based on 119 article reviews
Price from $9.99 to $1999.99
c18 sep pak cartridge - by Bioz Stars, 2020-09
93/100 stars

Images

1) Product Images from "Synthesis and preliminary evaluation of radiolabeled bis(zinc(II)-dipicolylamine) coordination complexes as cell death imaging agents"

Article Title: Synthesis and preliminary evaluation of radiolabeled bis(zinc(II)-dipicolylamine) coordination complexes as cell death imaging agents

Journal: Bioorganic & medicinal chemistry

doi: 10.1016/j.bmc.2011.04.029

Representative radiochromatograms of [ 99m Tc(CO) 3 - 1 ] 3+ (A) and 99m Tc-HYNIC- 1 (B) before C18 Sep-Pak purification.
Figure Legend Snippet: Representative radiochromatograms of [ 99m Tc(CO) 3 - 1 ] 3+ (A) and 99m Tc-HYNIC- 1 (B) before C18 Sep-Pak purification.

Techniques Used: Purification

2) Product Images from "Renal cell carcinoma-derived gangliosides suppress nuclear factor-?B activation in T cells"

Article Title: Renal cell carcinoma-derived gangliosides suppress nuclear factor-?B activation in T cells

Journal: Journal of Clinical Investigation

doi:

The product in tumor supernatant responsible for inhibiting κB binding activity weighs less than 3 kDa and is water soluble ( a ). Normal T cells were incubated with 1 of the following for 18 hours: media, unfractionated RCC supernatant, or the fractions that were less than 3 kDa or more than 3 kDa after passage through a YM3 Diaflo membrane. T cells were then stimulated for 2 hours with PMA (10 ng/mL) and ionomycin (0.75 μg/mL) before preparation of nuclear extract for EMSA using the κB probe. Similar findings were noted in 7 additional experiments ( b ). The less than 3-kDa fraction of the RCC supernatant was loaded onto a C18 Sep-Pak cartridge. Eluate was collected (fraction 1), and bound material was eluted with 15% ethanol (fraction 2), petroleum ether (fraction 3), and ethyl acetate (fraction 4). T cells were then incubated with medium, unfractionated RCC supernatant, or C18 fractions for 18 hours before stimulation with PMA/ionomycin for 2 hours. Nuclear extracts were harvested, and EMSA was performed. The results with fractions 2 and 3 were not shown because they were similar to those of fraction 4. Similar results were obtained in 3 additional experiments.
Figure Legend Snippet: The product in tumor supernatant responsible for inhibiting κB binding activity weighs less than 3 kDa and is water soluble ( a ). Normal T cells were incubated with 1 of the following for 18 hours: media, unfractionated RCC supernatant, or the fractions that were less than 3 kDa or more than 3 kDa after passage through a YM3 Diaflo membrane. T cells were then stimulated for 2 hours with PMA (10 ng/mL) and ionomycin (0.75 μg/mL) before preparation of nuclear extract for EMSA using the κB probe. Similar findings were noted in 7 additional experiments ( b ). The less than 3-kDa fraction of the RCC supernatant was loaded onto a C18 Sep-Pak cartridge. Eluate was collected (fraction 1), and bound material was eluted with 15% ethanol (fraction 2), petroleum ether (fraction 3), and ethyl acetate (fraction 4). T cells were then incubated with medium, unfractionated RCC supernatant, or C18 fractions for 18 hours before stimulation with PMA/ionomycin for 2 hours. Nuclear extracts were harvested, and EMSA was performed. The results with fractions 2 and 3 were not shown because they were similar to those of fraction 4. Similar results were obtained in 3 additional experiments.

Techniques Used: Binding Assay, Activity Assay, Incubation

3) Product Images from "Synthesis and preliminary evaluation of radiolabeled bis(zinc(II)-dipicolylamine) coordination complexes as cell death imaging agents"

Article Title: Synthesis and preliminary evaluation of radiolabeled bis(zinc(II)-dipicolylamine) coordination complexes as cell death imaging agents

Journal: Bioorganic & medicinal chemistry

doi: 10.1016/j.bmc.2011.04.029

Representative radiochromatograms of [ 99m Tc(CO) 3 - 1 ] 3+ (A) and 99m Tc-HYNIC- 1 (B) before C18 Sep-Pak purification.
Figure Legend Snippet: Representative radiochromatograms of [ 99m Tc(CO) 3 - 1 ] 3+ (A) and 99m Tc-HYNIC- 1 (B) before C18 Sep-Pak purification.

Techniques Used: Purification

4) Product Images from "Renal cell carcinoma-derived gangliosides suppress nuclear factor-?B activation in T cells"

Article Title: Renal cell carcinoma-derived gangliosides suppress nuclear factor-?B activation in T cells

Journal: Journal of Clinical Investigation

doi:

The product in tumor supernatant responsible for inhibiting κB binding activity weighs less than 3 kDa and is water soluble ( a ). Normal T cells were incubated with 1 of the following for 18 hours: media, unfractionated RCC supernatant, or the fractions that were less than 3 kDa or more than 3 kDa after passage through a YM3 Diaflo membrane. T cells were then stimulated for 2 hours with PMA (10 ng/mL) and ionomycin (0.75 μg/mL) before preparation of nuclear extract for EMSA using the κB probe. Similar findings were noted in 7 additional experiments ( b ). The less than 3-kDa fraction of the RCC supernatant was loaded onto a C18 Sep-Pak cartridge. Eluate was collected (fraction 1), and bound material was eluted with 15% ethanol (fraction 2), petroleum ether (fraction 3), and ethyl acetate (fraction 4). T cells were then incubated with medium, unfractionated RCC supernatant, or C18 fractions for 18 hours before stimulation with PMA/ionomycin for 2 hours. Nuclear extracts were harvested, and EMSA was performed. The results with fractions 2 and 3 were not shown because they were similar to those of fraction 4. Similar results were obtained in 3 additional experiments.
Figure Legend Snippet: The product in tumor supernatant responsible for inhibiting κB binding activity weighs less than 3 kDa and is water soluble ( a ). Normal T cells were incubated with 1 of the following for 18 hours: media, unfractionated RCC supernatant, or the fractions that were less than 3 kDa or more than 3 kDa after passage through a YM3 Diaflo membrane. T cells were then stimulated for 2 hours with PMA (10 ng/mL) and ionomycin (0.75 μg/mL) before preparation of nuclear extract for EMSA using the κB probe. Similar findings were noted in 7 additional experiments ( b ). The less than 3-kDa fraction of the RCC supernatant was loaded onto a C18 Sep-Pak cartridge. Eluate was collected (fraction 1), and bound material was eluted with 15% ethanol (fraction 2), petroleum ether (fraction 3), and ethyl acetate (fraction 4). T cells were then incubated with medium, unfractionated RCC supernatant, or C18 fractions for 18 hours before stimulation with PMA/ionomycin for 2 hours. Nuclear extracts were harvested, and EMSA was performed. The results with fractions 2 and 3 were not shown because they were similar to those of fraction 4. Similar results were obtained in 3 additional experiments.

Techniques Used: Binding Assay, Activity Assay, Incubation

5) Product Images from "Synthesis and preliminary evaluation of radiolabeled bis(zinc(II)-dipicolylamine) coordination complexes as cell death imaging agents"

Article Title: Synthesis and preliminary evaluation of radiolabeled bis(zinc(II)-dipicolylamine) coordination complexes as cell death imaging agents

Journal: Bioorganic & medicinal chemistry

doi: 10.1016/j.bmc.2011.04.029

Representative radiochromatograms of [ 99m Tc(CO) 3 - 1 ] 3+ (A) and 99m Tc-HYNIC- 1 (B) before C18 Sep-Pak purification.
Figure Legend Snippet: Representative radiochromatograms of [ 99m Tc(CO) 3 - 1 ] 3+ (A) and 99m Tc-HYNIC- 1 (B) before C18 Sep-Pak purification.

Techniques Used: Purification

Related Articles

Incubation:

Article Title: DNA repair of myeloma plasma cells correlates with clinical outcome: the effect of the nonhomologous end-joining inhibitor SCR7
Article Snippet: .. Briefly, melphalan (Sigma) was partially hydrolyzed by incubation in HCl, and reaction products were separated by chromatography using a C18 Sep-pak cartridge (Vac 35cm3 , 10-g capacity; Waters, Millipore Corp). .. Human MM cell lines (melphalan-sensitive, RPMI8226, and melphalan-resistant, LR5) were cultured in RPMI 1640 medium (Sigma-Aldrich) containing 10% (vol/vol) fetal bovine serum (Gibco), 1% (vol/vol) of 200 mM l -glutamine, 1% (vol/vol) of 10 mg/mL gentamicin (Gibco), and 1% (vol/vol) of 250 μg/mL amphotericin B (Sigma-Aldrich).

other:

Article Title: Fresh-Cut Pineapple as a New Carrier of Probiotic Lactic Acid Bacteria
Article Snippet: The homogenate was centrifuged at 10,000 rpm for 5 min and the supernatant was recovered, filtered through a C18 Sep-Pak cartridge (Waters, Milford, MA, USA) and then through a 0.2 μ m cellulose acetate filter.

Article Title: Renal cell carcinoma-derived gangliosides suppress nuclear factor-?B activation in T cells
Article Snippet: After acidification, the supernatants were loaded on a C18 Sep-Pak cartridge and subjected to stepwise elution.

Chromatography:

Article Title: DNA repair of myeloma plasma cells correlates with clinical outcome: the effect of the nonhomologous end-joining inhibitor SCR7
Article Snippet: .. Briefly, melphalan (Sigma) was partially hydrolyzed by incubation in HCl, and reaction products were separated by chromatography using a C18 Sep-pak cartridge (Vac 35cm3 , 10-g capacity; Waters, Millipore Corp). .. Human MM cell lines (melphalan-sensitive, RPMI8226, and melphalan-resistant, LR5) were cultured in RPMI 1640 medium (Sigma-Aldrich) containing 10% (vol/vol) fetal bovine serum (Gibco), 1% (vol/vol) of 200 mM l -glutamine, 1% (vol/vol) of 10 mg/mL gentamicin (Gibco), and 1% (vol/vol) of 250 μg/mL amphotericin B (Sigma-Aldrich).

Radioactivity:

Article Title: Synthesis and preliminary evaluation of radiolabeled bis(zinc(II)-dipicolylamine) coordination complexes as cell death imaging agents
Article Snippet: .. To obtain an injectable solution, the radiolabeling mixture was passed through a C18 Sep-Pak cartridge. ..

Purification:

Article Title: Synthesis and preliminary evaluation of radiolabeled bis(zinc(II)-dipicolylamine) coordination complexes as cell death imaging agents
Article Snippet: .. After cooling to room temperature, the reaction mixture was purified by passing it through a C18-Sep-Pak cartridge. ..

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  • 93
    Waters Corporation c18 cartridges
    Selection of iRT-peptides and definition of <t>iRT-C18</t> scale
    C18 Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 cartridges/product/Waters Corporation
    Average 93 stars, based on 148 article reviews
    Price from $9.99 to $1999.99
    c18 cartridges - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    89
    Waters Corporation sep pak light c18 cartridge
    (a) Purification of 89 Zr-DFO-LEM2/15 in a PD-10 column; the antibody elutes between volume fractions 2.5 and 5.0 ml. Inset: ITLC image illustrating RQP of the 89 Zr-DFO-LEM2/15 peak. 1 corresponds to the radioimmunoconjugate and 2 to the free radionuclide. (b) Percentage of bound radioactivity to LEM2/15 after 7-day incubation of 89 Zr-DFO-LEM2/15 with human serum at 37°C ( ) and 4°C ( ) and human plasma at 37°C ( ) and 4°C ( ); data expressed as mean ± SD. ( n =4). Representative HPLC chromatogram of 68 Ga-DOTA-AF7p-1 before (c) or after (d) to <t>Sep-Pak</t> Light <t>C18</t> cartridge purification. 68 Ga-DOTA-AF7p-1 is detected at 8.7 min by UV absorbance at 280 nm ( ) and radioactivity detector ( ).
    Sep Pak Light C18 Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 89/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sep pak light c18 cartridge/product/Waters Corporation
    Average 89 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    sep pak light c18 cartridge - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    91
    Waters Corporation sep pak c18
    Comparison of recoveries obtained for elution with three consecutive fractions of 2.5 mL methanol and total recovery from all fractions for 100-fold preconcentration of PFCAs from 250 mL solution containing 3.0 μg L −1 each analyte. Preconcentration carried out on 500 mg <t>Sep-Pak</t> <t>C18</t> sorbent bed
    Sep Pak C18, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sep pak c18/product/Waters Corporation
    Average 91 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    sep pak c18 - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    Selection of iRT-peptides and definition of iRT-C18 scale

    Journal: Proteomics

    Article Title: Using iRT, a normalized retention time for more targeted measurement of peptides

    doi: 10.1002/pmic.201100463

    Figure Lengend Snippet: Selection of iRT-peptides and definition of iRT-C18 scale

    Article Snippet: Peptides were acidified with 1% trifluoroacetic acid (TFA, Thermo Scientific) and purified by solid-phase extraction using C18 cartridges (Sep-Pak, Waters).

    Techniques: Selection

    Representative radiochromatograms of [ 99m Tc(CO) 3 - 1 ] 3+ (A) and 99m Tc-HYNIC- 1 (B) before C18 Sep-Pak purification.

    Journal: Bioorganic & medicinal chemistry

    Article Title: Synthesis and preliminary evaluation of radiolabeled bis(zinc(II)-dipicolylamine) coordination complexes as cell death imaging agents

    doi: 10.1016/j.bmc.2011.04.029

    Figure Lengend Snippet: Representative radiochromatograms of [ 99m Tc(CO) 3 - 1 ] 3+ (A) and 99m Tc-HYNIC- 1 (B) before C18 Sep-Pak purification.

    Article Snippet: The reaction was stopped by addition of 1 mL H2 O and the reaction mixture was sent over a C18 Sep-Pak cartridge (Waters Corp., Milford, MA) washed with 5 mL of H2 O, and eluted with 1 mL of ethanol.

    Techniques: Purification

    (a) Purification of 89 Zr-DFO-LEM2/15 in a PD-10 column; the antibody elutes between volume fractions 2.5 and 5.0 ml. Inset: ITLC image illustrating RQP of the 89 Zr-DFO-LEM2/15 peak. 1 corresponds to the radioimmunoconjugate and 2 to the free radionuclide. (b) Percentage of bound radioactivity to LEM2/15 after 7-day incubation of 89 Zr-DFO-LEM2/15 with human serum at 37°C ( ) and 4°C ( ) and human plasma at 37°C ( ) and 4°C ( ); data expressed as mean ± SD. ( n =4). Representative HPLC chromatogram of 68 Ga-DOTA-AF7p-1 before (c) or after (d) to Sep-Pak Light C18 cartridge purification. 68 Ga-DOTA-AF7p-1 is detected at 8.7 min by UV absorbance at 280 nm ( ) and radioactivity detector ( ).

    Journal: Contrast Media & Molecular Imaging

    Article Title: MT1-MMP as a PET Imaging Biomarker for Pancreas Cancer Management

    doi: 10.1155/2018/8382148

    Figure Lengend Snippet: (a) Purification of 89 Zr-DFO-LEM2/15 in a PD-10 column; the antibody elutes between volume fractions 2.5 and 5.0 ml. Inset: ITLC image illustrating RQP of the 89 Zr-DFO-LEM2/15 peak. 1 corresponds to the radioimmunoconjugate and 2 to the free radionuclide. (b) Percentage of bound radioactivity to LEM2/15 after 7-day incubation of 89 Zr-DFO-LEM2/15 with human serum at 37°C ( ) and 4°C ( ) and human plasma at 37°C ( ) and 4°C ( ); data expressed as mean ± SD. ( n =4). Representative HPLC chromatogram of 68 Ga-DOTA-AF7p-1 before (c) or after (d) to Sep-Pak Light C18 cartridge purification. 68 Ga-DOTA-AF7p-1 is detected at 8.7 min by UV absorbance at 280 nm ( ) and radioactivity detector ( ).

    Article Snippet: The radiochromatogram of the reaction mixture prior to passing through the Sep-Pak Light C18 cartridge showed two distinct peaks corresponding to free 68 Ga and 68 Ga-DOTA-AF7p-1 with retention times of 2.5 and 8.7 min, respectively. shows a representative chromatogram of peptide labelling with a low yield aiming at demonstrating that even in those cases, a final product can be successfully obtained.

    Techniques: Purification, Radioactivity, Incubation, High Performance Liquid Chromatography

    Comparison of recoveries obtained for elution with three consecutive fractions of 2.5 mL methanol and total recovery from all fractions for 100-fold preconcentration of PFCAs from 250 mL solution containing 3.0 μg L −1 each analyte. Preconcentration carried out on 500 mg Sep-Pak C18 sorbent bed

    Journal: Mikrochimica Acta

    Article Title: HPLC determination of perfluorinated carboxylic acids with fluorescence detection

    doi: 10.1007/s00604-010-0513-z

    Figure Lengend Snippet: Comparison of recoveries obtained for elution with three consecutive fractions of 2.5 mL methanol and total recovery from all fractions for 100-fold preconcentration of PFCAs from 250 mL solution containing 3.0 μg L −1 each analyte. Preconcentration carried out on 500 mg Sep-Pak C18 sorbent bed

    Article Snippet: Solid phase extraction procedures PFCAs were preconcentrated using Oasis-WAX (30, 60 and 120 mg, 30 μm) and Sep-Pak C18 (500 mg) cartridges purchased from Waters (Miliford, USA).

    Techniques: