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Phenomenex c18 column
Chromatograms of Medicago truncatula seed extract. A. Injection into a <t>C18</t> column (250 × 25 mm, 5 μm, Phenomenex) on an Agilent 1200 HPLC apparatus. The flow was 3.5 mL.min -1 . The gradient was H 2 O + 0.04%TFA (solvent A) / ACN +0.04% TFA (solvent B) 90/10 for 5 min, then 60% solvent B for 25 min. The elution was monitored using a diode array detector at 210 nm. B. The fraction purified in A was injected into the same column with an elution under isocratic conditions (solvent B 30%). Peaks containing entomotoxic activity are indicated.
C18 Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 94/100, based on 886 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "High toxicity and specificity of the saponin 3-GlcA-28-AraRhaxyl-medicagenate, from Medicago truncatula seeds, for Sitophilus oryzae"

Article Title: High toxicity and specificity of the saponin 3-GlcA-28-AraRhaxyl-medicagenate, from Medicago truncatula seeds, for Sitophilus oryzae

Journal: BMC Chemical Biology

doi: 10.1186/1472-6769-12-3

Chromatograms of Medicago truncatula seed extract. A. Injection into a C18 column (250 × 25 mm, 5 μm, Phenomenex) on an Agilent 1200 HPLC apparatus. The flow was 3.5 mL.min -1 . The gradient was H 2 O + 0.04%TFA (solvent A) / ACN +0.04% TFA (solvent B) 90/10 for 5 min, then 60% solvent B for 25 min. The elution was monitored using a diode array detector at 210 nm. B. The fraction purified in A was injected into the same column with an elution under isocratic conditions (solvent B 30%). Peaks containing entomotoxic activity are indicated.
Figure Legend Snippet: Chromatograms of Medicago truncatula seed extract. A. Injection into a C18 column (250 × 25 mm, 5 μm, Phenomenex) on an Agilent 1200 HPLC apparatus. The flow was 3.5 mL.min -1 . The gradient was H 2 O + 0.04%TFA (solvent A) / ACN +0.04% TFA (solvent B) 90/10 for 5 min, then 60% solvent B for 25 min. The elution was monitored using a diode array detector at 210 nm. B. The fraction purified in A was injected into the same column with an elution under isocratic conditions (solvent B 30%). Peaks containing entomotoxic activity are indicated.

Techniques Used: Injection, High Performance Liquid Chromatography, Flow Cytometry, Purification, Activity Assay

2) Product Images from "Metabolic engineering of Kluyveromyces lactis for L-ascorbic acid (vitamin C) biosynthesis"

Article Title: Metabolic engineering of Kluyveromyces lactis for L-ascorbic acid (vitamin C) biosynthesis

Journal: Microbial Cell Factories

doi: 10.1186/1475-2859-12-59

Intracellular ascorbate-like compounds and L-AA produced by K. lactis strains harboring plant genes. ( A ) Transformed yeast cells were grown on cheese whey, mineral medium (0.67% [w/v] YNB) and on rich (YP) medium (20 g.L -1 peptone, 10 g.L -1 Yeast extract) supplemented with 2% (w/v) D-galactose or lactose, for 48 h (initial OD 600 0.05). ( B ) Measurement of L-AA by HPLC Analysis. The retention time for L-AA and D-DAL were 11.175 and 12.003 min respectively when applying a C18 column with 99:1 H 2 O/acetic acid as mobile phase (upper B ). The strains were grown in YP medium supplemented with 2% (w/v) galactose for 48 hours. CBS2359 parental strain was taken as control.
Figure Legend Snippet: Intracellular ascorbate-like compounds and L-AA produced by K. lactis strains harboring plant genes. ( A ) Transformed yeast cells were grown on cheese whey, mineral medium (0.67% [w/v] YNB) and on rich (YP) medium (20 g.L -1 peptone, 10 g.L -1 Yeast extract) supplemented with 2% (w/v) D-galactose or lactose, for 48 h (initial OD 600 0.05). ( B ) Measurement of L-AA by HPLC Analysis. The retention time for L-AA and D-DAL were 11.175 and 12.003 min respectively when applying a C18 column with 99:1 H 2 O/acetic acid as mobile phase (upper B ). The strains were grown in YP medium supplemented with 2% (w/v) galactose for 48 hours. CBS2359 parental strain was taken as control.

Techniques Used: Produced, Transformation Assay, High Performance Liquid Chromatography

3) Product Images from "Cell migration inhibition activity of a non-RGD disintegrin from Crotalus durissus collilineatus venom"

Article Title: Cell migration inhibition activity of a non-RGD disintegrin from Crotalus durissus collilineatus venom

Journal: The Journal of Venomous Animals and Toxins Including Tropical Diseases

doi: 10.1186/s40409-018-0167-6

Chromatographic profiles of non-RGD disintegrin from C. d. collilineatus venom using RP-FPLC system. a C. d. collilineatus venom (30 mg) was applied on a C18 column (250 × 10 mm, 5 μm particles, 300 Å), at a flow rate of 5 mL/min and ( b ) Fraction 2 (200 μg) on a C18 column (250 × 4.6 mm, 3.6 μm particles), at a flow rate of 0.5 mL/min. Elution in both chromatograms was carried out in a segmented concentration gradient from 6.3 to 100% of solution B (80% ACN in 0.1% TFA, represented by the blue dashed line) and absorbance was monitored at 214 nm. Inset panel – whole chromatographic profile without magnification
Figure Legend Snippet: Chromatographic profiles of non-RGD disintegrin from C. d. collilineatus venom using RP-FPLC system. a C. d. collilineatus venom (30 mg) was applied on a C18 column (250 × 10 mm, 5 μm particles, 300 Å), at a flow rate of 5 mL/min and ( b ) Fraction 2 (200 μg) on a C18 column (250 × 4.6 mm, 3.6 μm particles), at a flow rate of 0.5 mL/min. Elution in both chromatograms was carried out in a segmented concentration gradient from 6.3 to 100% of solution B (80% ACN in 0.1% TFA, represented by the blue dashed line) and absorbance was monitored at 214 nm. Inset panel – whole chromatographic profile without magnification

Techniques Used: Fast Protein Liquid Chromatography, Flow Cytometry, Concentration Assay

4) Product Images from "The Venom of the Spider Selenocosmia Jiafu Contains Various Neurotoxins Acting on Voltage-Gated Ion Channels in Rat Dorsal Root Ganglion Neurons"

Article Title: The Venom of the Spider Selenocosmia Jiafu Contains Various Neurotoxins Acting on Voltage-Gated Ion Channels in Rat Dorsal Root Ganglion Neurons

Journal: Toxins

doi: 10.3390/toxins6030988

The complexity of the venom peptides: ( A ) reversed-phase high-performance liquid chromatography (RP-HPLC) separation of 1 mg of soluble venom from S . jiafu in an analytical C18 column equilibrated with solution A (distilled water in 0.1% TFA), using a gradient from 0% to 50% of solution B (acetonitrile in 0.1% TFA) over 50 min with a flow rate of 1 mL/min. Absorbance was read at 215 nm. ( B ) Matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) of S . jiafu venom. ( C ) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the venom of the spider S . jiafu . 1: Marker; 2 and 3: Venom.
Figure Legend Snippet: The complexity of the venom peptides: ( A ) reversed-phase high-performance liquid chromatography (RP-HPLC) separation of 1 mg of soluble venom from S . jiafu in an analytical C18 column equilibrated with solution A (distilled water in 0.1% TFA), using a gradient from 0% to 50% of solution B (acetonitrile in 0.1% TFA) over 50 min with a flow rate of 1 mL/min. Absorbance was read at 215 nm. ( B ) Matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) of S . jiafu venom. ( C ) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the venom of the spider S . jiafu . 1: Marker; 2 and 3: Venom.

Techniques Used: High Performance Liquid Chromatography, Flow Cytometry, Mass Spectrometry, Polyacrylamide Gel Electrophoresis, SDS Page, Marker

5) Product Images from "Anticonvulsant Effects of Fractions Isolated from Dinoponera quadriceps (Kempt) Ant Venom (Formicidae: Ponerinae)"

Article Title: Anticonvulsant Effects of Fractions Isolated from Dinoponera quadriceps (Kempt) Ant Venom (Formicidae: Ponerinae)

Journal: Toxins

doi: 10.3390/toxins9010005

Dinoponera quadriceps crude venom chromatography. ( A ) Chromatographic profile (reverse-phase high performance liquid chromatography—Hitachi system, Phenomenex C18 column 2.6 × 25 cm, 12 µm, 300 Å) of crude Dinoponera quadriceps venom, showing six major fractions monitored at 210 (gray) and 280 (black) nm and eluted using a linear gradient from acetonitrile containing trifluoroacetic acid at 0.1% (TFA) (100% ACN/H 2 O v / v ) for 100 min; ( B ) ESI mass spectrum (LC/ESI-MS—Waters system, mass range between 200 and 2000 m / z , nitrogen gas flow rate of 4.1 L·h −1 , capillary voltage of 2.3 kV, cone voltage of 32 V, extractor voltage of 8 V, source heater set at 100 °C, solvent heater set at 400 °C, ion voltage of 1.0 V, and a multiplier voltage of 800 V) of the fractions of DqTx1 to DqTx6.
Figure Legend Snippet: Dinoponera quadriceps crude venom chromatography. ( A ) Chromatographic profile (reverse-phase high performance liquid chromatography—Hitachi system, Phenomenex C18 column 2.6 × 25 cm, 12 µm, 300 Å) of crude Dinoponera quadriceps venom, showing six major fractions monitored at 210 (gray) and 280 (black) nm and eluted using a linear gradient from acetonitrile containing trifluoroacetic acid at 0.1% (TFA) (100% ACN/H 2 O v / v ) for 100 min; ( B ) ESI mass spectrum (LC/ESI-MS—Waters system, mass range between 200 and 2000 m / z , nitrogen gas flow rate of 4.1 L·h −1 , capillary voltage of 2.3 kV, cone voltage of 32 V, extractor voltage of 8 V, source heater set at 100 °C, solvent heater set at 400 °C, ion voltage of 1.0 V, and a multiplier voltage of 800 V) of the fractions of DqTx1 to DqTx6.

Techniques Used: Chromatography, High Performance Liquid Chromatography, Mass Spectrometry, Flow Cytometry

6) Product Images from "Nature's Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain"

Article Title: Nature's Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain

Journal: Marine Drugs

doi: 10.3390/md12041699

UV chromatogram of an extract of Streptomyces strain HB202 (at 250 nm) after fermentation in GYM4 medium for three days and extraction with EtOAc. For analysis, a RP-C18 column was used applying an H 2 O/CH 3 CN gradient on a VWR Hitachi Elite LaChrom system. Peak detection was done by DAD/MS. Numbers indicate identified streptophenazines. 1 , 2 , 3 : new streptophenazines (I–K), 4 : streptophenazine A, 5 : streptophenazine B, 6 : streptophenazine C, 7 : streptophenazine D, 8 : streptophenazine F, 9 : streptophenazine G, 10 : streptophenazine H, *: unknown streptophenazines ( > 10 different molecules).
Figure Legend Snippet: UV chromatogram of an extract of Streptomyces strain HB202 (at 250 nm) after fermentation in GYM4 medium for three days and extraction with EtOAc. For analysis, a RP-C18 column was used applying an H 2 O/CH 3 CN gradient on a VWR Hitachi Elite LaChrom system. Peak detection was done by DAD/MS. Numbers indicate identified streptophenazines. 1 , 2 , 3 : new streptophenazines (I–K), 4 : streptophenazine A, 5 : streptophenazine B, 6 : streptophenazine C, 7 : streptophenazine D, 8 : streptophenazine F, 9 : streptophenazine G, 10 : streptophenazine H, *: unknown streptophenazines ( > 10 different molecules).

Techniques Used: Mass Spectrometry

7) Product Images from "Wound Healing Activity and Mechanisms of Action of an Antibacterial Protein from the Venom of the Eastern Diamondback Rattlesnake (Crotalus adamanteus)"

Article Title: Wound Healing Activity and Mechanisms of Action of an Antibacterial Protein from the Venom of the Eastern Diamondback Rattlesnake (Crotalus adamanteus)

Journal: PLoS ONE

doi: 10.1371/journal.pone.0080199

Purification of Crotalus adamanteus toxin-II (CaTx-II) from Eastern Diamondback Rattlesnake venom. (A) High Performance Liquid Chromatography (HPLC) profiles of C. adamanteus crude venom from a Superdex G-75 column, (B–D) Reverse-phase (RP)-HPLC chromatograms from Sepharose C18 and C8 columns, (E) Molecular mass of pure CaTx-II, (F) Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) profile of RP-HPLC fractions, lanes indicate: CA-CV C. adamanteus crude venom (1–2), lane (4) homogeneity of CaTx-II confirmed by SDS-PAGE as 15 kDa of CA-F1 - reverse-phase fraction and (5) marker, 25 µg of protein loaded per lane, respectively.
Figure Legend Snippet: Purification of Crotalus adamanteus toxin-II (CaTx-II) from Eastern Diamondback Rattlesnake venom. (A) High Performance Liquid Chromatography (HPLC) profiles of C. adamanteus crude venom from a Superdex G-75 column, (B–D) Reverse-phase (RP)-HPLC chromatograms from Sepharose C18 and C8 columns, (E) Molecular mass of pure CaTx-II, (F) Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) profile of RP-HPLC fractions, lanes indicate: CA-CV C. adamanteus crude venom (1–2), lane (4) homogeneity of CaTx-II confirmed by SDS-PAGE as 15 kDa of CA-F1 - reverse-phase fraction and (5) marker, 25 µg of protein loaded per lane, respectively.

Techniques Used: Purification, High Performance Liquid Chromatography, Polyacrylamide Gel Electrophoresis, SDS Page, Marker

8) Product Images from "Subtype Specificity of β-Toxin Tf1a from Tityus fasciolatus in Voltage Gated Sodium Channels"

Article Title: Subtype Specificity of β-Toxin Tf1a from Tityus fasciolatus in Voltage Gated Sodium Channels

Journal: Toxins

doi: 10.3390/toxins10090339

Chromatographic profile of Tityus fasciolatus venom. Chromatographic profile of 1 mg of Tityus fasciolatus crude venom using the RP-HPLC C18 column at 1 mL/min flow rate monitored at 216 nm. The fraction of interest, highlighted in the large image as Tf1a, eluted at 41.1 min (~41.1% acetonitrile). ( A ) 0.5%[B]/min. ( B ) Second purification step with 0.5%[B]/min at 45 °C. ( C ) Last purification step with 0.3%[B]/min at 45 °C.
Figure Legend Snippet: Chromatographic profile of Tityus fasciolatus venom. Chromatographic profile of 1 mg of Tityus fasciolatus crude venom using the RP-HPLC C18 column at 1 mL/min flow rate monitored at 216 nm. The fraction of interest, highlighted in the large image as Tf1a, eluted at 41.1 min (~41.1% acetonitrile). ( A ) 0.5%[B]/min. ( B ) Second purification step with 0.5%[B]/min at 45 °C. ( C ) Last purification step with 0.3%[B]/min at 45 °C.

Techniques Used: High Performance Liquid Chromatography, Flow Cytometry, Purification

9) Product Images from "Antibacterial Activity of the Non-Cytotoxic Peptide (p-BthTX-I)2 and Its Serum Degradation Product against Multidrug-Resistant Bacteria"

Article Title: Antibacterial Activity of the Non-Cytotoxic Peptide (p-BthTX-I)2 and Its Serum Degradation Product against Multidrug-Resistant Bacteria

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

doi: 10.3390/molecules22111898

Serum stability assays by analytical high-performance liquid chromatography (HPLC). ( A ) p-BthTX-I and ( B ) (p-BthTX-I) 2 were incubated with human serum for the indicated times. Analytical HPLC was performed on a Shimadzu (Kyoto, Japan) system using a C18 column (4.6 × 150 mm, Phenomenex) with a linear gradient of solvent B (5–95%) at 1 mL/min for 15 min (Solvent A: 0.045% trifluoroacetic acid (TFA) in water. Solvent B: 0.036% TFA in acetonitrile). Marked boxes in the figures show the same degradation product produced by both the peptides.
Figure Legend Snippet: Serum stability assays by analytical high-performance liquid chromatography (HPLC). ( A ) p-BthTX-I and ( B ) (p-BthTX-I) 2 were incubated with human serum for the indicated times. Analytical HPLC was performed on a Shimadzu (Kyoto, Japan) system using a C18 column (4.6 × 150 mm, Phenomenex) with a linear gradient of solvent B (5–95%) at 1 mL/min for 15 min (Solvent A: 0.045% trifluoroacetic acid (TFA) in water. Solvent B: 0.036% TFA in acetonitrile). Marked boxes in the figures show the same degradation product produced by both the peptides.

Techniques Used: High Performance Liquid Chromatography, Incubation, Produced

Analytical HPLC comparing the peaks of the peptides (p-BthTX-I) 2 , des-Lys 12 /Lys 13 -(p-BthTX-I) 2 , and des-Lys 1 /Lys 2 -(p-BthTX-I) 2 to the RT of peaks of the stable serum degradation product of (p-BthTX-I) 2 . Samples were eluted at 5 min and 3 h. Analytical HPLC was performed on a Shimadzu system using a C18 column (4.6 × 150 mm, Phenomenex) with a linear gradient of solvent B (5–95%) at 1 mL/min for 30 min (Solvent A: 0.045% trifluoroacetic acid (TFA) in water. Solvent B: 0.036% TFA in acetonitrile). Marked box indicates the peak of des-Lys 12 /Lys 13 -(p-BthTX-I) 2 and the stable degradation peptide produced.
Figure Legend Snippet: Analytical HPLC comparing the peaks of the peptides (p-BthTX-I) 2 , des-Lys 12 /Lys 13 -(p-BthTX-I) 2 , and des-Lys 1 /Lys 2 -(p-BthTX-I) 2 to the RT of peaks of the stable serum degradation product of (p-BthTX-I) 2 . Samples were eluted at 5 min and 3 h. Analytical HPLC was performed on a Shimadzu system using a C18 column (4.6 × 150 mm, Phenomenex) with a linear gradient of solvent B (5–95%) at 1 mL/min for 30 min (Solvent A: 0.045% trifluoroacetic acid (TFA) in water. Solvent B: 0.036% TFA in acetonitrile). Marked box indicates the peak of des-Lys 12 /Lys 13 -(p-BthTX-I) 2 and the stable degradation peptide produced.

Techniques Used: High Performance Liquid Chromatography, Produced

10) Product Images from "One-Step 18F-Labeling of Estradiol Derivative for PET Imaging of Breast Cancer"

Article Title: One-Step 18F-Labeling of Estradiol Derivative for PET Imaging of Breast Cancer

Journal: Contrast Media & Molecular Imaging

doi: 10.1155/2018/5362329

Radio-HPLC analysis of [ 18 F]AmBF 3 -TEG-ES ( t R = 16.3 min) before purification (a) and after C18 purification (b).
Figure Legend Snippet: Radio-HPLC analysis of [ 18 F]AmBF 3 -TEG-ES ( t R = 16.3 min) before purification (a) and after C18 purification (b).

Techniques Used: High Performance Liquid Chromatography, Purification

11) Product Images from "Vitamin B2 as a virulence factor in Pseudogymnoascus destructans skin infection"

Article Title: Vitamin B2 as a virulence factor in Pseudogymnoascus destructans skin infection

Journal: Scientific Reports

doi: 10.1038/srep33200

Production of riboflavin in Pseudogymnoascus destructans . ( a ) HPLC chromatogram of SPE C18 extract from liquid cultivation medium of P. destructans strain 20631-21 T (see Fig. 6a,b for medium and blank controls). ( b ) Average production curve (±standard error) of riboflavin in six strains of P. destructans ( Fig. 3 ) shows continuous metabolite accumulation. ( c ) Average production curve of [Fe 3+ ] triacetylfusarinine C in six strains of P. destructans peaks after 6 to 8 weeks of culture. The concentration of the metabolite was corrected for biomass content in the fermentation medium.
Figure Legend Snippet: Production of riboflavin in Pseudogymnoascus destructans . ( a ) HPLC chromatogram of SPE C18 extract from liquid cultivation medium of P. destructans strain 20631-21 T (see Fig. 6a,b for medium and blank controls). ( b ) Average production curve (±standard error) of riboflavin in six strains of P. destructans ( Fig. 3 ) shows continuous metabolite accumulation. ( c ) Average production curve of [Fe 3+ ] triacetylfusarinine C in six strains of P. destructans peaks after 6 to 8 weeks of culture. The concentration of the metabolite was corrected for biomass content in the fermentation medium.

Techniques Used: High Performance Liquid Chromatography, Concentration Assay

Production and tolerance of riboflavin in Pseudogymnoascus fungi. ( a ) HPLC chromatogram of SPE C18 extract from liquid cultivation medium of Pseudogymnoascus sp. strain CCF5026. ( b ) Blank liquid cultivation medium HPLC chromatogram. ( c ) Average production curves (±standard error) of riboflavin in non-pathogenic Pseudogymnoascus strains. The concentration of the metabolite was corrected for biomass content in the fermentation medium. ( d ) Tolerance of P. destructans strains to riboflavin in concentrations ranging from 0 to 200 μg ml −1 . Riboflavin was added after autoclaving the medium containing glucose (20 g l −1 ), yeast extract (5 g l −1 ), and agar (20 g l −1 ). Colony diameter was measured after 49 days. Three replicates were used in each experiment, and their values may overlap.
Figure Legend Snippet: Production and tolerance of riboflavin in Pseudogymnoascus fungi. ( a ) HPLC chromatogram of SPE C18 extract from liquid cultivation medium of Pseudogymnoascus sp. strain CCF5026. ( b ) Blank liquid cultivation medium HPLC chromatogram. ( c ) Average production curves (±standard error) of riboflavin in non-pathogenic Pseudogymnoascus strains. The concentration of the metabolite was corrected for biomass content in the fermentation medium. ( d ) Tolerance of P. destructans strains to riboflavin in concentrations ranging from 0 to 200 μg ml −1 . Riboflavin was added after autoclaving the medium containing glucose (20 g l −1 ), yeast extract (5 g l −1 ), and agar (20 g l −1 ). Colony diameter was measured after 49 days. Three replicates were used in each experiment, and their values may overlap.

Techniques Used: High Performance Liquid Chromatography, Concentration Assay

12) Product Images from "High toxicity and specificity of the saponin 3-GlcA-28-AraRhaxyl-medicagenate, from Medicago truncatula seeds, for Sitophilus oryzae"

Article Title: High toxicity and specificity of the saponin 3-GlcA-28-AraRhaxyl-medicagenate, from Medicago truncatula seeds, for Sitophilus oryzae

Journal: BMC Chemical Biology

doi: 10.1186/1472-6769-12-3

Chromatograms of Medicago truncatula seed extract. A. Injection into a C18 column (250 × 25 mm, 5 μm, Phenomenex) on an Agilent 1200 HPLC apparatus. The flow was 3.5 mL.min -1 . The gradient was H 2 O + 0.04%TFA (solvent A) / ACN +0.04% TFA (solvent B) 90/10 for 5 min, then 60% solvent B for 25 min. The elution was monitored using a diode array detector at 210 nm. B. The fraction purified in A was injected into the same column with an elution under isocratic conditions (solvent B 30%). Peaks containing entomotoxic activity are indicated.
Figure Legend Snippet: Chromatograms of Medicago truncatula seed extract. A. Injection into a C18 column (250 × 25 mm, 5 μm, Phenomenex) on an Agilent 1200 HPLC apparatus. The flow was 3.5 mL.min -1 . The gradient was H 2 O + 0.04%TFA (solvent A) / ACN +0.04% TFA (solvent B) 90/10 for 5 min, then 60% solvent B for 25 min. The elution was monitored using a diode array detector at 210 nm. B. The fraction purified in A was injected into the same column with an elution under isocratic conditions (solvent B 30%). Peaks containing entomotoxic activity are indicated.

Techniques Used: Injection, High Performance Liquid Chromatography, Flow Cytometry, Purification, Activity Assay

13) Product Images from "Deep sequencing analysis of toad Rhinella schneideri skin glands and partial biochemical characterization of its cutaneous secretion"

Article Title: Deep sequencing analysis of toad Rhinella schneideri skin glands and partial biochemical characterization of its cutaneous secretion

Journal: The Journal of Venomous Animals and Toxins Including Tropical Diseases

doi: 10.1186/s40409-018-0173-8

Cutaneous secretion chromatogram in C18 column RP-FPLC and the SDS-PAGE profile of each fraction. The blue line represents the absorbance monitored at 214 nm and the green line represents the concentration of solution B. Each fraction was analyzed in SDS-PAGE stained with silver (insert figures). Insert figure a represents fractions CS1 to CS13 and insert figure b shows fractions CS14 to CS26. The wells at left show the low molecular weight marker from GE Healthcare
Figure Legend Snippet: Cutaneous secretion chromatogram in C18 column RP-FPLC and the SDS-PAGE profile of each fraction. The blue line represents the absorbance monitored at 214 nm and the green line represents the concentration of solution B. Each fraction was analyzed in SDS-PAGE stained with silver (insert figures). Insert figure a represents fractions CS1 to CS13 and insert figure b shows fractions CS14 to CS26. The wells at left show the low molecular weight marker from GE Healthcare

Techniques Used: Fast Protein Liquid Chromatography, SDS Page, Concentration Assay, Staining, Molecular Weight, Marker

14) Product Images from "Introgression of Swertia mussotii gene into Bupleurum scorzonerifolium via somatic hybridization"

Article Title: Introgression of Swertia mussotii gene into Bupleurum scorzonerifolium via somatic hybridization

Journal: BMC Plant Biology

doi: 10.1186/1471-2229-11-71

HPLC analysis of hybrid clones . A, Standard preparations of swertiamarin, gentiopicroside and mangiferin; B, S. mussotii ; C, B. scorzonerifolium ; D, Hybrid clone B24; E, Hybrid clone C18. UV spectrum of swertiamarin and mangiferin from samples were indicated in D and E. 1, S. mussotii ; 2, Standard preparations; 3, Hybrid clone B24; 4, Hybrid clone C18.
Figure Legend Snippet: HPLC analysis of hybrid clones . A, Standard preparations of swertiamarin, gentiopicroside and mangiferin; B, S. mussotii ; C, B. scorzonerifolium ; D, Hybrid clone B24; E, Hybrid clone C18. UV spectrum of swertiamarin and mangiferin from samples were indicated in D and E. 1, S. mussotii ; 2, Standard preparations; 3, Hybrid clone B24; 4, Hybrid clone C18.

Techniques Used: High Performance Liquid Chromatography, Clone Assay

15) Product Images from "Deep sequencing analysis of toad Rhinella schneideri skin glands and partial biochemical characterization of its cutaneous secretion"

Article Title: Deep sequencing analysis of toad Rhinella schneideri skin glands and partial biochemical characterization of its cutaneous secretion

Journal: The Journal of Venomous Animals and Toxins Including Tropical Diseases

doi: 10.1186/s40409-018-0173-8

Cutaneous secretion chromatogram in C18 column RP-FPLC and the SDS-PAGE profile of each fraction. The blue line represents the absorbance monitored at 214 nm and the green line represents the concentration of solution B. Each fraction was analyzed in SDS-PAGE stained with silver (insert figures). Insert figure a represents fractions CS1 to CS13 and insert figure b shows fractions CS14 to CS26. The wells at left show the low molecular weight marker from GE Healthcare
Figure Legend Snippet: Cutaneous secretion chromatogram in C18 column RP-FPLC and the SDS-PAGE profile of each fraction. The blue line represents the absorbance monitored at 214 nm and the green line represents the concentration of solution B. Each fraction was analyzed in SDS-PAGE stained with silver (insert figures). Insert figure a represents fractions CS1 to CS13 and insert figure b shows fractions CS14 to CS26. The wells at left show the low molecular weight marker from GE Healthcare

Techniques Used: Fast Protein Liquid Chromatography, SDS Page, Concentration Assay, Staining, Molecular Weight, Marker

16) Product Images from "Cell migration inhibition activity of a non-RGD disintegrin from Crotalus durissus collilineatus venom"

Article Title: Cell migration inhibition activity of a non-RGD disintegrin from Crotalus durissus collilineatus venom

Journal: The Journal of Venomous Animals and Toxins Including Tropical Diseases

doi: 10.1186/s40409-018-0167-6

Chromatographic profiles of non-RGD disintegrin from C. d. collilineatus venom using RP-FPLC system. a C. d. collilineatus venom (30 mg) was applied on a C18 column (250 × 10 mm, 5 μm particles, 300 Å), at a flow rate of 5 mL/min and ( b ) Fraction 2 (200 μg) on a C18 column (250 × 4.6 mm, 3.6 μm particles), at a flow rate of 0.5 mL/min. Elution in both chromatograms was carried out in a segmented concentration gradient from 6.3 to 100% of solution B (80% ACN in 0.1% TFA, represented by the blue dashed line) and absorbance was monitored at 214 nm. Inset panel – whole chromatographic profile without magnification
Figure Legend Snippet: Chromatographic profiles of non-RGD disintegrin from C. d. collilineatus venom using RP-FPLC system. a C. d. collilineatus venom (30 mg) was applied on a C18 column (250 × 10 mm, 5 μm particles, 300 Å), at a flow rate of 5 mL/min and ( b ) Fraction 2 (200 μg) on a C18 column (250 × 4.6 mm, 3.6 μm particles), at a flow rate of 0.5 mL/min. Elution in both chromatograms was carried out in a segmented concentration gradient from 6.3 to 100% of solution B (80% ACN in 0.1% TFA, represented by the blue dashed line) and absorbance was monitored at 214 nm. Inset panel – whole chromatographic profile without magnification

Techniques Used: Fast Protein Liquid Chromatography, Flow Cytometry, Concentration Assay

17) Product Images from "An [18F]-Positron-Emitting, Fluorescent, Cerebrospinal Fluid Probe for Imaging Damage to the Brain and Spine"

Article Title: An [18F]-Positron-Emitting, Fluorescent, Cerebrospinal Fluid Probe for Imaging Damage to the Brain and Spine

Journal: Theranostics

doi: 10.7150/thno.19408

Spectral and radioactive properties of [ 18/19 F]- 2. (A) Excitation and emission spectra of 5-6 μM solutions of fluorescein (black) and [ 18/19 F]- 2 (green) in 1x PBS, pH 7.4 measured on a Cary Eclipse spectrophotometer, with 5 nm slit widths, and excitation at 490 nm. (B) In vitro cell viability tests performed with compound 2 (red line) and fluorescein (control) 24 hour on U87, Hela, MDA-MB-231, and A549 immortal cell lines using a CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS, G3582, Promega). Toxicity is not observed. (C) UV-Vis (280, 350, and 450 nm) UPLC-MS of 2 on a waters Acuity UPLC and a Phenomenex Luna Kinetex 1.7 µm EVO C18 50 x 2.1 mm column (00B-4726-AN), with a 1.5 min, a10-90% H 2 O:ACN (0.05% TFA) elution gradient indicating a pure synthesis of 2 . (D) Reverse phase HPLC of radiolabeled [ 18 F]- 2 on a Varian HPLC, using an Waters SunfireTM C18 3.5 μm 4.6 x 50 mm column (186002551), a10-90% H 2 O:ACN (0.05% TFA) elution gradient and a flow rate of 2 mL/min. (E) 10 µL 3x dilution series of [ 18/19 F]- 2 beginning at 70 µM (1.7 pmols) and 170 µCi of [ 18/19 F]- 2 (first spot). A 10 µl volume was plated onto glass-backed TLC plate model. Imaging was performed using (i) bright-field; (ii) a UV-sight hand-held 4.5 volt LED black-light (fluorescence); (iii) a Bruker Xtreme optical imaging device (fluorescence: 1 sec acquisition using excitation and emission filters set at λex= 450 nm and λem= 535 nm); (iv) and a phosphorimager.
Figure Legend Snippet: Spectral and radioactive properties of [ 18/19 F]- 2. (A) Excitation and emission spectra of 5-6 μM solutions of fluorescein (black) and [ 18/19 F]- 2 (green) in 1x PBS, pH 7.4 measured on a Cary Eclipse spectrophotometer, with 5 nm slit widths, and excitation at 490 nm. (B) In vitro cell viability tests performed with compound 2 (red line) and fluorescein (control) 24 hour on U87, Hela, MDA-MB-231, and A549 immortal cell lines using a CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS, G3582, Promega). Toxicity is not observed. (C) UV-Vis (280, 350, and 450 nm) UPLC-MS of 2 on a waters Acuity UPLC and a Phenomenex Luna Kinetex 1.7 µm EVO C18 50 x 2.1 mm column (00B-4726-AN), with a 1.5 min, a10-90% H 2 O:ACN (0.05% TFA) elution gradient indicating a pure synthesis of 2 . (D) Reverse phase HPLC of radiolabeled [ 18 F]- 2 on a Varian HPLC, using an Waters SunfireTM C18 3.5 μm 4.6 x 50 mm column (186002551), a10-90% H 2 O:ACN (0.05% TFA) elution gradient and a flow rate of 2 mL/min. (E) 10 µL 3x dilution series of [ 18/19 F]- 2 beginning at 70 µM (1.7 pmols) and 170 µCi of [ 18/19 F]- 2 (first spot). A 10 µl volume was plated onto glass-backed TLC plate model. Imaging was performed using (i) bright-field; (ii) a UV-sight hand-held 4.5 volt LED black-light (fluorescence); (iii) a Bruker Xtreme optical imaging device (fluorescence: 1 sec acquisition using excitation and emission filters set at λex= 450 nm and λem= 535 nm); (iv) and a phosphorimager.

Techniques Used: Spectrophotometry, In Vitro, Multiple Displacement Amplification, Proliferation Assay, Mass Spectrometry, High Performance Liquid Chromatography, Flow Cytometry, Thin Layer Chromatography, Imaging, Fluorescence, Optical Imaging, Size-exclusion Chromatography

18) Product Images from "High levels of anti-inflammatory and pro-resolving lipid mediators lipoxins and resolvins and declining docosahexaenoic acid levels in human milk during the first month of lactation"

Article Title: High levels of anti-inflammatory and pro-resolving lipid mediators lipoxins and resolvins and declining docosahexaenoic acid levels in human milk during the first month of lactation

Journal: Lipids in Health and Disease

doi: 10.1186/1476-511X-12-89

Changes in omega-6 and omega-3 fatty acids in human milk over the first month of lactation. Relative amount of the omega-6 PUFA ( E ) AA (C20:4) and its precursors ( A ) C18:2 and ( C ) γC18:3. Relative amount of the omega-3 PUFA ( F) DHA (C22:6) and its precursors ( D ) αC18:3 and ( B ) EPA (C20:5). For an overview on PUFA fatty acid biosynthesis see Figure 3 .
Figure Legend Snippet: Changes in omega-6 and omega-3 fatty acids in human milk over the first month of lactation. Relative amount of the omega-6 PUFA ( E ) AA (C20:4) and its precursors ( A ) C18:2 and ( C ) γC18:3. Relative amount of the omega-3 PUFA ( F) DHA (C22:6) and its precursors ( D ) αC18:3 and ( B ) EPA (C20:5). For an overview on PUFA fatty acid biosynthesis see Figure 3 .

Techniques Used:

19) Product Images from "Skin gland concentrations adapted to different evolutionary pressures in the head and posterior regions of the caecilian Siphonops annulatus"

Article Title: Skin gland concentrations adapted to different evolutionary pressures in the head and posterior regions of the caecilian Siphonops annulatus

Journal: Scientific Reports

doi: 10.1038/s41598-018-22005-5

Biochemical characterization of the secretion extracted from the head and posterior region of Siphonops annulatus . ( a for the original image). The numbers on the left refer to the molecular mass markers (kDa) shown in the left column. Main differences between the two types of secretion are indicated by arrows. ( b ) C18-RP-HPLC profiles of secretions extracted from the head (red) and from the posterior region (black). The insert represents a high magnification of the image.
Figure Legend Snippet: Biochemical characterization of the secretion extracted from the head and posterior region of Siphonops annulatus . ( a for the original image). The numbers on the left refer to the molecular mass markers (kDa) shown in the left column. Main differences between the two types of secretion are indicated by arrows. ( b ) C18-RP-HPLC profiles of secretions extracted from the head (red) and from the posterior region (black). The insert represents a high magnification of the image.

Techniques Used: High Performance Liquid Chromatography

20) Product Images from "Biotransformation of Flavonoid Conjugates with Fatty Acids and Evaluations of Their Functionalities"

Article Title: Biotransformation of Flavonoid Conjugates with Fatty Acids and Evaluations of Their Functionalities

Journal: Frontiers in Pharmacology

doi: 10.3389/fphar.2017.00759

DPPH scavenging activities in naringin, NHDC, and grapefruit extracts and their FA esters; C12-lauric acid, C18:1-oleic acid, C18:3-linolenic acid, and ω-3 PUFA rich esters (each in 0.1 μmol).
Figure Legend Snippet: DPPH scavenging activities in naringin, NHDC, and grapefruit extracts and their FA esters; C12-lauric acid, C18:1-oleic acid, C18:3-linolenic acid, and ω-3 PUFA rich esters (each in 0.1 μmol).

Techniques Used:

21) Product Images from "One-Step 18F-Labeling of Estradiol Derivative for PET Imaging of Breast Cancer"

Article Title: One-Step 18F-Labeling of Estradiol Derivative for PET Imaging of Breast Cancer

Journal: Contrast Media & Molecular Imaging

doi: 10.1155/2018/5362329

Radio-HPLC analysis of [ 18 F]AmBF 3 -TEG-ES ( t R = 16.3 min) before purification (a) and after C18 purification (b).
Figure Legend Snippet: Radio-HPLC analysis of [ 18 F]AmBF 3 -TEG-ES ( t R = 16.3 min) before purification (a) and after C18 purification (b).

Techniques Used: High Performance Liquid Chromatography, Purification

22) Product Images from "Skin gland concentrations adapted to different evolutionary pressures in the head and posterior regions of the caecilian Siphonops annulatus"

Article Title: Skin gland concentrations adapted to different evolutionary pressures in the head and posterior regions of the caecilian Siphonops annulatus

Journal: Scientific Reports

doi: 10.1038/s41598-018-22005-5

Biochemical characterization of the secretion extracted from the head and posterior region of Siphonops annulatus . ( a ) SDS-PAGE of the secretion of the head (H) and of the posterior region (P) (See Fig. 4S for the original image). The numbers on the left refer to the molecular mass markers (kDa) shown in the left column. Main differences between the two types of secretion are indicated by arrows. ( b ) C18-RP-HPLC profiles of secretions extracted from the head (red) and from the posterior region (black). The insert represents a high magnification of the image.
Figure Legend Snippet: Biochemical characterization of the secretion extracted from the head and posterior region of Siphonops annulatus . ( a ) SDS-PAGE of the secretion of the head (H) and of the posterior region (P) (See Fig. 4S for the original image). The numbers on the left refer to the molecular mass markers (kDa) shown in the left column. Main differences between the two types of secretion are indicated by arrows. ( b ) C18-RP-HPLC profiles of secretions extracted from the head (red) and from the posterior region (black). The insert represents a high magnification of the image.

Techniques Used: SDS Page, High Performance Liquid Chromatography

23) Product Images from "The 32 kDa Enamelin Undergoes Conformational Transitions upon Calcium Binding"

Article Title: The 32 kDa Enamelin Undergoes Conformational Transitions upon Calcium Binding

Journal:

doi: 10.1016/j.jsb.2008.04.007

Characterization of the purified 32 kDa enamelin. (A) RP-HPLC elution profile after purification with first a C4 and then a C18 column. SDS-PAGE gel of the purified protein stained with stains-all (SA) and coomassie brilliant blue (CBB). Note that lane
Figure Legend Snippet: Characterization of the purified 32 kDa enamelin. (A) RP-HPLC elution profile after purification with first a C4 and then a C18 column. SDS-PAGE gel of the purified protein stained with stains-all (SA) and coomassie brilliant blue (CBB). Note that lane

Techniques Used: Purification, High Performance Liquid Chromatography, SDS Page, Staining

24) Product Images from "Peptide Nanoparticle Delivery of Charge-Neutral Splice-Switching Morpholino Oligonucleotides"

Article Title: Peptide Nanoparticle Delivery of Charge-Neutral Splice-Switching Morpholino Oligonucleotides

Journal: Nucleic Acid Therapeutics

doi: 10.1089/nat.2014.0511

Exon skipping activity in H2K mdx myotubes by 1 μM PMO complexed with different lipid-conjugated RXR4 at different ratios. Lipid length ranges from C12 (lauroyl) to C18 (stearoyl). Results are from at least three experiments performed in duplicate.
Figure Legend Snippet: Exon skipping activity in H2K mdx myotubes by 1 μM PMO complexed with different lipid-conjugated RXR4 at different ratios. Lipid length ranges from C12 (lauroyl) to C18 (stearoyl). Results are from at least three experiments performed in duplicate.

Techniques Used: Activity Assay

25) Product Images from "Partial purification and functional characterization of Ts19 Frag-I, a novel toxin from Tityus serrulatus scorpion venom"

Article Title: Partial purification and functional characterization of Ts19 Frag-I, a novel toxin from Tityus serrulatus scorpion venom

Journal: The Journal of Venomous Animals and Toxins Including Tropical Diseases

doi: 10.1186/s40409-015-0051-6

Rechromatography of the subfractions eluted from the fractions VIIIA and VIIIB. ( a ) Subfraction 7. ( b ) Subfraction 9. The C18 column (2.1 mm × 250.0 mm, 3.6 μm particles, Phenomenex) was equilibrated with 0.1 % TFA and the proteins were eluted using a concentration gradient from 0 to 100 % of solution B (80 % ACN in 0.1 % TFA), represented by the dashed line. Absorbance was monitored at 214 nm, at 25 °C, using a FPLC Äkta Purifier UPC-10 system. Fractions of 0.3 mL/tube were collected at a flow rate of 0.4 mL/min
Figure Legend Snippet: Rechromatography of the subfractions eluted from the fractions VIIIA and VIIIB. ( a ) Subfraction 7. ( b ) Subfraction 9. The C18 column (2.1 mm × 250.0 mm, 3.6 μm particles, Phenomenex) was equilibrated with 0.1 % TFA and the proteins were eluted using a concentration gradient from 0 to 100 % of solution B (80 % ACN in 0.1 % TFA), represented by the dashed line. Absorbance was monitored at 214 nm, at 25 °C, using a FPLC Äkta Purifier UPC-10 system. Fractions of 0.3 mL/tube were collected at a flow rate of 0.4 mL/min

Techniques Used: Concentration Assay, Fast Protein Liquid Chromatography, Flow Cytometry

Chromatographic profiles of fractions VIIIA and VIIIB from Tsv. ( a ) Fraction VIIIA. ( b ) Fraction VIIIB. Fractions (4 mg, eluted of the cation exchange chromatography from Tityus serrulatus venom) were submitted to RP-FPLC on a C18 column (4.6 mm × 250.0 mm, 5 μm particles, Shimadzu Corp.). The column was equilibrated with 0.1 % trifluoroacetic acid (TFA) and the proteins were eluted using a concentration gradient from 0 to 100 % of solution B (80 % acetonitrile (ACN) in 0.1 % TFA), represented by the dashed line. Absorbance was monitored at 214 nm, at 25 °C, using an FPLC Äkta Purifier UPC-10 system. Fractions of 0.3 mL/tube were collected at a flow rate of 0.7 mL/min
Figure Legend Snippet: Chromatographic profiles of fractions VIIIA and VIIIB from Tsv. ( a ) Fraction VIIIA. ( b ) Fraction VIIIB. Fractions (4 mg, eluted of the cation exchange chromatography from Tityus serrulatus venom) were submitted to RP-FPLC on a C18 column (4.6 mm × 250.0 mm, 5 μm particles, Shimadzu Corp.). The column was equilibrated with 0.1 % trifluoroacetic acid (TFA) and the proteins were eluted using a concentration gradient from 0 to 100 % of solution B (80 % acetonitrile (ACN) in 0.1 % TFA), represented by the dashed line. Absorbance was monitored at 214 nm, at 25 °C, using an FPLC Äkta Purifier UPC-10 system. Fractions of 0.3 mL/tube were collected at a flow rate of 0.7 mL/min

Techniques Used: Chromatography, Fast Protein Liquid Chromatography, Concentration Assay, Flow Cytometry

Related Articles

High Performance Liquid Chromatography:

Article Title: Metabolic engineering of Kluyveromyces lactis for L-ascorbic acid (vitamin C) biosynthesis
Article Snippet: .. The identity and L-AA measurements were achieved by high performance liquid chromatography with Luna 5u C18 column (250 × 4.6 mm, Phenomenex) with 99:1 H2O/acetic acid as eluent, a flow rate of 0.5 mL.min-1 , and UV detection set at 254 nm and the L-AA content was calculated using the L-AA standard curve. .. The L-AA (cat. nº A5960) and D-DAL (cat. nº 58320) standard curve was made using reagents from sigma Aldrich.

Article Title: Subtype Specificity of β-Toxin Tf1a from Tityus fasciolatus in Voltage Gated Sodium Channels
Article Snippet: .. Toxin Purification Crude venom of Tityus fasciolatus was fractioned by RP-HPLC (Reversed Phase High Performance Liquid Chromatography) (Shimadzu Co., Kyoto, Japan), using a C18 column (Synergi Fusion RP 4 μ, 80 Å, 250 × 4.6 mm (Phenomenex, Inc., Torrance, CA, USA). .. Components were separated using a linear gradient of solvent A (0.12% TFA in water) and solvent B (0.10% TFA in acetonitrile) from 0 to 60% for 60 min at a 1 mL/min flow rate as described previously [ ].

Article Title: Nature's Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain
Article Snippet: .. Analytical reversed phase HPLC-DAD/MS experiments were performed using a C18 column (Phenomenex Onyx Monolithic C18, 100 × 3.00 mm) applying an H2 O (A)/CH3 CN (B) gradient with 0.1% formic acid added to both solvents (gradient: 0 min 5% B, 4 min 60% B, 6 min 100% B; flow 2 mL/min) on a VWR Hitachi Elite LaChrom system (DAD-detector: Hitachi L-2450 diode array detector) coupled to an ESI-ion trap detector with positive ionization (Esquire 4000, Bruker Daltonics). .. Semi-preparative HPLC-DAD was carried out using a Phenomenex normal phase column (Luna 5U Silica (2), 100A, 250 × 10.00 mm, 5 micron).

Flow Cytometry:

Article Title: Metabolic engineering of Kluyveromyces lactis for L-ascorbic acid (vitamin C) biosynthesis
Article Snippet: .. The identity and L-AA measurements were achieved by high performance liquid chromatography with Luna 5u C18 column (250 × 4.6 mm, Phenomenex) with 99:1 H2O/acetic acid as eluent, a flow rate of 0.5 mL.min-1 , and UV detection set at 254 nm and the L-AA content was calculated using the L-AA standard curve. .. The L-AA (cat. nº A5960) and D-DAL (cat. nº 58320) standard curve was made using reagents from sigma Aldrich.

Article Title: Wound Healing Activity and Mechanisms of Action of an Antibacterial Protein from the Venom of the Eastern Diamondback Rattlesnake (Crotalus adamanteus)
Article Snippet: .. All the fractions were tested for enzymatic and antibacterial effects, and the fraction that showed the highest enzymatic and antibacterial effects were then subjected to reverse-phased liquid chromatography (RP-HPLC) via a C18 column (4.6×150 mm, Phenomenex, Upsala, Sweden), using a linear gradient of aqueous acetonitrile (80%) in 0.1% trifluoroacetic acid (Sigma Aldrich Co, St Louis, Missouri, USA) at a flow rate of 1.0 ml per min. .. Subsequent purification of active fractions were derived by using a C8 RP-HPLC column (100 Å, 4.6×250 mm) .

Article Title: Cell migration inhibition activity of a non-RGD disintegrin from Crotalus durissus collilineatus venom
Article Snippet: .. The supernatant was fractionated on a C18 column (250 × 10 mm, 5 μm particles, 300 Å, Phenomenex, Torrence, CA, USA) at a flow rate of 5 mL/min, using the concentration gradient described by Calvete et al. [ ]. .. The second step was performed on another C18 column (250 × 4.6 mm, 3.6 μm particles, Phenomenex, Torrence, CA, USA) at a flow rate of 1 mL/min and the proteins were eluted using a segmented concentration gradient from 6.3 to 100% of solution B (80% acetonitrile, ACN, in 0.1% TFA).

Article Title: Nature's Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain
Article Snippet: .. Analytical reversed phase HPLC-DAD/MS experiments were performed using a C18 column (Phenomenex Onyx Monolithic C18, 100 × 3.00 mm) applying an H2 O (A)/CH3 CN (B) gradient with 0.1% formic acid added to both solvents (gradient: 0 min 5% B, 4 min 60% B, 6 min 100% B; flow 2 mL/min) on a VWR Hitachi Elite LaChrom system (DAD-detector: Hitachi L-2450 diode array detector) coupled to an ESI-ion trap detector with positive ionization (Esquire 4000, Bruker Daltonics). .. Semi-preparative HPLC-DAD was carried out using a Phenomenex normal phase column (Luna 5U Silica (2), 100A, 250 × 10.00 mm, 5 micron).

Purification:

Article Title: Subtype Specificity of β-Toxin Tf1a from Tityus fasciolatus in Voltage Gated Sodium Channels
Article Snippet: .. Toxin Purification Crude venom of Tityus fasciolatus was fractioned by RP-HPLC (Reversed Phase High Performance Liquid Chromatography) (Shimadzu Co., Kyoto, Japan), using a C18 column (Synergi Fusion RP 4 μ, 80 Å, 250 × 4.6 mm (Phenomenex, Inc., Torrance, CA, USA). .. Components were separated using a linear gradient of solvent A (0.12% TFA in water) and solvent B (0.10% TFA in acetonitrile) from 0 to 60% for 60 min at a 1 mL/min flow rate as described previously [ ].

Concentration Assay:

Article Title: Cell migration inhibition activity of a non-RGD disintegrin from Crotalus durissus collilineatus venom
Article Snippet: .. The supernatant was fractionated on a C18 column (250 × 10 mm, 5 μm particles, 300 Å, Phenomenex, Torrence, CA, USA) at a flow rate of 5 mL/min, using the concentration gradient described by Calvete et al. [ ]. .. The second step was performed on another C18 column (250 × 4.6 mm, 3.6 μm particles, Phenomenex, Torrence, CA, USA) at a flow rate of 1 mL/min and the proteins were eluted using a segmented concentration gradient from 6.3 to 100% of solution B (80% acetonitrile, ACN, in 0.1% TFA).

Injection:

Article Title: High toxicity and specificity of the saponin 3-GlcA-28-AraRhaxyl-medicagenate, from Medicago truncatula seeds, for Sitophilus oryzae
Article Snippet: .. The extract was injected into a C18 column (250 x 4.1 mm, 3 μm, Phenomenex). ..

Liquid Chromatography:

Article Title: Wound Healing Activity and Mechanisms of Action of an Antibacterial Protein from the Venom of the Eastern Diamondback Rattlesnake (Crotalus adamanteus)
Article Snippet: .. All the fractions were tested for enzymatic and antibacterial effects, and the fraction that showed the highest enzymatic and antibacterial effects were then subjected to reverse-phased liquid chromatography (RP-HPLC) via a C18 column (4.6×150 mm, Phenomenex, Upsala, Sweden), using a linear gradient of aqueous acetonitrile (80%) in 0.1% trifluoroacetic acid (Sigma Aldrich Co, St Louis, Missouri, USA) at a flow rate of 1.0 ml per min. .. Subsequent purification of active fractions were derived by using a C8 RP-HPLC column (100 Å, 4.6×250 mm) .

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  • 94
    Phenomenex c18 column
    Chromatograms of Medicago truncatula seed extract. A. Injection into a <t>C18</t> column (250 × 25 mm, 5 μm, Phenomenex) on an Agilent 1200 HPLC apparatus. The flow was 3.5 mL.min -1 . The gradient was H 2 O + 0.04%TFA (solvent A) / ACN +0.04% TFA (solvent B) 90/10 for 5 min, then 60% solvent B for 25 min. The elution was monitored using a diode array detector at 210 nm. B. The fraction purified in A was injected into the same column with an elution under isocratic conditions (solvent B 30%). Peaks containing entomotoxic activity are indicated.
    C18 Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 94/100, based on 886 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 column/product/Phenomenex
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    86
    Phenomenex c18 reverse phase trap columns
    FOXA2 is an acetylated protein. (A) LC-MS/MS spectrum and the deduced peptide sequence of FOXA2 acetylation at lysine K275 (in red). (B) Peptides containing five in vivo FOXA2 acetylation sites which were identified by LC-MS/MS analyses. FLAG-FOXA2 was expressed in human kidney HEK293T cells, purified and digested with proteases. Peptides were separated and enriched by <t>C18</t> analytical columns and subsequently subjected to LC-MS/MS. Acetylated residues in red and underlined. (C) Schematic representation of the FOXA2 protein structure. Markers illustrate the localization of the five acetylation sites and colored boxes represent functional domains. TAD, Trans Activation Domain . NLS, Nuclear Localization Sequence .
    C18 Reverse Phase Trap Columns, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Phenomenex reverse phase c18 hplc column
    Demonstration of oxidized PS species in apoptotic membranes using negative ion LC/MS/MS. The presence of oxidized PAPS or PLPS species in lipid extracts of apoptotic cells was analyzed by <t>HPLC</t> with on-line negative ion electrospray ionization tandem mass spectrometry. Separation was done on a reverse phase <t>C18</t> column (2.1 × 250 mm, 5μm) using methanol/water as mobile phase at a flow rate of 0.2 ml/min with gradient and oxidized PLPS (a) and PAPS (b) molecular species identified by their characteristic retention time and daughter ions specific for each analyte, as described in Materials and methods.
    Reverse Phase C18 Hplc Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Phenomenex hr esi qtof ms analysis
    Extracted ion counts chromatograms from <t>LC/HR-ESI-QTOF-MS</t> analysis of pepteridines A (left panel) and B (right panel) from the butanol extracts of P-form culture broth (top), standard compounds (middle), and co-injection (bottom). DOI: http://dx.doi.org/10.7554/eLife.25229.027
    Hr Esi Qtof Ms Analysis, supplied by Phenomenex, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Chromatograms of Medicago truncatula seed extract. A. Injection into a C18 column (250 × 25 mm, 5 μm, Phenomenex) on an Agilent 1200 HPLC apparatus. The flow was 3.5 mL.min -1 . The gradient was H 2 O + 0.04%TFA (solvent A) / ACN +0.04% TFA (solvent B) 90/10 for 5 min, then 60% solvent B for 25 min. The elution was monitored using a diode array detector at 210 nm. B. The fraction purified in A was injected into the same column with an elution under isocratic conditions (solvent B 30%). Peaks containing entomotoxic activity are indicated.

    Journal: BMC Chemical Biology

    Article Title: High toxicity and specificity of the saponin 3-GlcA-28-AraRhaxyl-medicagenate, from Medicago truncatula seeds, for Sitophilus oryzae

    doi: 10.1186/1472-6769-12-3

    Figure Lengend Snippet: Chromatograms of Medicago truncatula seed extract. A. Injection into a C18 column (250 × 25 mm, 5 μm, Phenomenex) on an Agilent 1200 HPLC apparatus. The flow was 3.5 mL.min -1 . The gradient was H 2 O + 0.04%TFA (solvent A) / ACN +0.04% TFA (solvent B) 90/10 for 5 min, then 60% solvent B for 25 min. The elution was monitored using a diode array detector at 210 nm. B. The fraction purified in A was injected into the same column with an elution under isocratic conditions (solvent B 30%). Peaks containing entomotoxic activity are indicated.

    Article Snippet: The extract was injected into a C18 column (250 x 4.1 mm, 3 μm, Phenomenex).

    Techniques: Injection, High Performance Liquid Chromatography, Flow Cytometry, Purification, Activity Assay

    FOXA2 is an acetylated protein. (A) LC-MS/MS spectrum and the deduced peptide sequence of FOXA2 acetylation at lysine K275 (in red). (B) Peptides containing five in vivo FOXA2 acetylation sites which were identified by LC-MS/MS analyses. FLAG-FOXA2 was expressed in human kidney HEK293T cells, purified and digested with proteases. Peptides were separated and enriched by C18 analytical columns and subsequently subjected to LC-MS/MS. Acetylated residues in red and underlined. (C) Schematic representation of the FOXA2 protein structure. Markers illustrate the localization of the five acetylation sites and colored boxes represent functional domains. TAD, Trans Activation Domain . NLS, Nuclear Localization Sequence .

    Journal: PLoS ONE

    Article Title: SIRT1 Mediates FOXA2 Breakdown by Deacetylation in a Nutrient-Dependent Manner

    doi: 10.1371/journal.pone.0098438

    Figure Lengend Snippet: FOXA2 is an acetylated protein. (A) LC-MS/MS spectrum and the deduced peptide sequence of FOXA2 acetylation at lysine K275 (in red). (B) Peptides containing five in vivo FOXA2 acetylation sites which were identified by LC-MS/MS analyses. FLAG-FOXA2 was expressed in human kidney HEK293T cells, purified and digested with proteases. Peptides were separated and enriched by C18 analytical columns and subsequently subjected to LC-MS/MS. Acetylated residues in red and underlined. (C) Schematic representation of the FOXA2 protein structure. Markers illustrate the localization of the five acetylation sites and colored boxes represent functional domains. TAD, Trans Activation Domain . NLS, Nuclear Localization Sequence .

    Article Snippet: Samples were subjected to nanoflow LC (Eksigent) using C18 reverse phase trap columns (Synergi 4 µm Hydro-RP 80 Å, Phenomenex; column dimensions 2 cm×100 µm, packed in-house) and subsequently separated on C18 analytical columns (ReproSil-Pur 120 C18-AQ, 5 µm, Dr. Maisch GmbH; column dimensions, 20 cm×50 µm; packed in-house) using a linear gradient from 0 to 40% buffer B (buffer A = 5% (v/v) acetonitrile, 0.1% formic acid (v/v); buffer B = 95% (v/v) acetonitrile, 0.1% formic acid (v/v)) in 60 min at a constant flow rate of 150 nl/min.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Sequencing, In Vivo, Purification, Functional Assay, Activation Assay

    Demonstration of oxidized PS species in apoptotic membranes using negative ion LC/MS/MS. The presence of oxidized PAPS or PLPS species in lipid extracts of apoptotic cells was analyzed by HPLC with on-line negative ion electrospray ionization tandem mass spectrometry. Separation was done on a reverse phase C18 column (2.1 × 250 mm, 5μm) using methanol/water as mobile phase at a flow rate of 0.2 ml/min with gradient and oxidized PLPS (a) and PAPS (b) molecular species identified by their characteristic retention time and daughter ions specific for each analyte, as described in Materials and methods.

    Journal: The Journal of Experimental Medicine

    Article Title: Oxidized phosphatidylserine-CD36 interactions play an essential role in macrophage-dependent phagocytosis of apoptotic cells

    doi: 10.1084/jem.20060370

    Figure Lengend Snippet: Demonstration of oxidized PS species in apoptotic membranes using negative ion LC/MS/MS. The presence of oxidized PAPS or PLPS species in lipid extracts of apoptotic cells was analyzed by HPLC with on-line negative ion electrospray ionization tandem mass spectrometry. Separation was done on a reverse phase C18 column (2.1 × 250 mm, 5μm) using methanol/water as mobile phase at a flow rate of 0.2 ml/min with gradient and oxidized PLPS (a) and PAPS (b) molecular species identified by their characteristic retention time and daughter ions specific for each analyte, as described in Materials and methods.

    Article Snippet: Each sample preparation (in 90% methanol) was injected onto a reverse phase C18 HPLC column (2 × 150 mm, 5 μm; ODS; Phenomenex) at a flow rate of 0.2 ml/min. oxPSes were resolved using a gradient from water containing 0.2% ammonium to methanol containing 0.2% ammonium.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Papanicolaou Stain, High Performance Liquid Chromatography, Flow Cytometry

    Extracted ion counts chromatograms from LC/HR-ESI-QTOF-MS analysis of pepteridines A (left panel) and B (right panel) from the butanol extracts of P-form culture broth (top), standard compounds (middle), and co-injection (bottom). DOI: http://dx.doi.org/10.7554/eLife.25229.027

    Journal: eLife

    Article Title: Genome mining unearths a hybrid nonribosomal peptide synthetase-like-pteridine synthase biosynthetic gene cluster

    doi: 10.7554/eLife.25229

    Figure Lengend Snippet: Extracted ion counts chromatograms from LC/HR-ESI-QTOF-MS analysis of pepteridines A (left panel) and B (right panel) from the butanol extracts of P-form culture broth (top), standard compounds (middle), and co-injection (bottom). DOI: http://dx.doi.org/10.7554/eLife.25229.027

    Article Snippet: The samples were subsequently resuspended in 5 ml methanol and 1–2 µl of each sample was injected for HR-ESI-QTOF-MS analysis (Column: Phenomenex Kinetex C18 (100 Å) 5 µm (4.6 × 250 mm) column; flow rate: 0.7 ml/min; mobile phase composition: water:acetonitrile (ACN) gradient solvent system containing 0.1% formic acid: 0–30 min, 5–100% ACN; hold for 5 min, 100% ACN; 0.1 min, 100–5% ACN; hold for 1.9 min, 5% ACN; 6.1 min re-equilibration post-time, 5% ACN).

    Techniques: Mass Spectrometry, Injection