Journal: Pharmacology Research & Perspectives
Article Title: Cyp1b1‐mediated suppression of lymphoid progenitors in bone marrow by polycyclic aromatic hydrocarbons coordinately impacts spleen and thymus: a selective role for the Ah Receptor. Cyp1b1‐mediated suppression of lymphoid progenitors in bone marrow by polycyclic aromatic hydrocarbons coordinately impacts spleen and thymus: a selective role for the Ah Receptor
Figure Lengend Snippet: Experimental design to evaluate polycyclic aromatic hydrocarbon ( PAH ) effects on cell populations in bone marrow (BM), spleen, and thymus . (A) The scheme for development of cell lineages from BM hematopoietic stem cells (HSC). Standard abbreviations are used for progenitors (long‐term HSC, LT‐HSC; short‐term HSC, ST‐HSC; common lymphoid progenitor, CLP; common myeloid progenitor, CMP; megakaryocyte‐erythroid progenitor, MEP; granulocyte‐macrophage progenitor, GMP) as defined in the Introduction. B220+ cells are B‐lymphocytes that migrate to the spleen for further maturation. CLP T are lymphoid progenitors modified for export to the thymus as circulating thymic progenitors (CTP). (B) Time‐dependent clearance of circulating dimethylbenz(a)anthracene ( DMBA), administered to wild type (WT) mice by Intraperitoneal (IP) injection or oral gavage (50 mg/kg in olive oil). Blood was collected by cardiac puncture and DMBA concentration measured by HPLC. The results represent the average of 2–3 experiments with n = 3–4 mice per assay.
Article Snippet: Residual PAH was extracted (2:1, acetone:acetonitrile) and separated by reverse‐phase HPLC (Beckman C18 Ultrasphere column), using a Waters 2695 Separation module interfaced to a Waters 470 scanning Fluorescence Detector, incorporating a 50–100% methanol gradient over a 30 min period.
Techniques: Modification, Mouse Assay, Injection, Concentration Assay, High Performance Liquid Chromatography