c18 column  (Beckman Coulter)


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    Structured Review

    Beckman Coulter c18 column
    C18 Column, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 column/product/Beckman Coulter
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    c18 column - by Bioz Stars, 2020-09
    92/100 stars

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    High Performance Liquid Chromatography:

    Article Title: Sampling of MHC class I-associated peptidome suggests relatively looser global association of HLA-B*5101 with peptides 1
    Article Snippet: .. The lyophilized peptides were resuspended in (A) H2 0/0.1% TFA and analyzed by reverse-phase HPLC using a C18 column (Vydac, Hesperia, CA; 4.6 x 250 mm) and Beckman 126 System Gold, equipped with programmable solvent module and variable wavelength detector 165 (Beckman Coulter, Inc., Fullerton, CA, USA). .. The analog signal was converted to digital signal using a single channel A/D converter and data acquisition software using PeakSimple Chromatography software (SRI Instruments, Torrance, CA, USA).

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    Beckman Coulter c18 reversed phase ultrasphere analytical column
    HPLC chromatograms of a mixture of no-carrier added 64 Cu-SB-TE1A1P and nat Cu-SB-TE1A1P (A) Radiation detector and (B) UV detector RP-HPLC using a <t>C18</t> reversed-phase <t>Ultrasphere</t> analytical column (5 µm, 4.6 mm × 25 cm) at 1 mL/min using the following conditions: 100% H 2 O for 5 min followed by a gradient from that point to 1:9 CH 3 CN:H 2 O over 10 min.
    C18 Reversed Phase Ultrasphere Analytical Column, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 reversed phase ultrasphere analytical column/product/Beckman Coulter
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    88
    Beckman Coulter c18 reversed phase hplc column
    Purification of Kbot21 from scorpion Buthus occitanus tunetanus venom. (A) Chromatography of fraction BotG50 on semi preparative C8 reverse phase <t>HPLC</t> column. (B) Chromatography of fraction B1on <t>C18-RP-HPLC,</t> B’1 was collected at 29min. (C) Kbot21 was purified from the fraction B’1 by C18-RP-HPLC. It is collected at 21min.
    C18 Reversed Phase Hplc Column, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 reversed phase hplc column/product/Beckman Coulter
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    93
    Beckman Coulter reverse phase hplc
    Experimental design to evaluate polycyclic aromatic hydrocarbon ( <t>PAH</t> ) effects on cell populations in bone marrow (BM), spleen, and thymus . (A) The scheme for development of cell lineages from BM hematopoietic stem cells (HSC). Standard abbreviations are used for progenitors (long‐term HSC, LT‐HSC; short‐term HSC, ST‐HSC; common lymphoid progenitor, CLP; common myeloid progenitor, CMP; megakaryocyte‐erythroid progenitor, MEP; granulocyte‐macrophage progenitor, GMP) as defined in the Introduction. B220+ cells are B‐lymphocytes that migrate to the spleen for further maturation. CLP T are lymphoid progenitors modified for export to the thymus as circulating thymic progenitors (CTP). (B) Time‐dependent clearance of circulating dimethylbenz(a)anthracene ( DMBA), administered to wild type (WT) mice by Intraperitoneal (IP) injection or oral gavage (50 mg/kg in olive oil). Blood was collected by cardiac puncture and DMBA concentration measured by <t>HPLC.</t> The results represent the average of 2–3 experiments with n = 3–4 mice per assay.
    Reverse Phase Hplc, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 93/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Beckman Coulter by high performance liquid chromatography hplc
    Representative <t>HPLC</t> chromatograms from in vitro incubations with pooled hepatic microsomal fractions from <t>ellipticine-pretreated</t> WT mice with ellipticine (ELLI) and either NADPH or NADH as cofactor. M1: 9-hydroxyellipticine; M2: 12-hydroxyellipticine; M3: 13-hydroxyellipticine. Phenacetin (PA) was used as internal standard.
    By High Performance Liquid Chromatography Hplc, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HPLC chromatograms of a mixture of no-carrier added 64 Cu-SB-TE1A1P and nat Cu-SB-TE1A1P (A) Radiation detector and (B) UV detector RP-HPLC using a C18 reversed-phase Ultrasphere analytical column (5 µm, 4.6 mm × 25 cm) at 1 mL/min using the following conditions: 100% H 2 O for 5 min followed by a gradient from that point to 1:9 CH 3 CN:H 2 O over 10 min.

    Journal: Bioorganic & medicinal chemistry

    Article Title: A Novel Side-Bridged Hybrid Phosphonate/Acetate Pendant Cyclam: Synthesis, Characterization, and 64Cu Small Animal PET Imaging

    doi: 10.1016/j.bmc.2008.11.073

    Figure Lengend Snippet: HPLC chromatograms of a mixture of no-carrier added 64 Cu-SB-TE1A1P and nat Cu-SB-TE1A1P (A) Radiation detector and (B) UV detector RP-HPLC using a C18 reversed-phase Ultrasphere analytical column (5 µm, 4.6 mm × 25 cm) at 1 mL/min using the following conditions: 100% H 2 O for 5 min followed by a gradient from that point to 1:9 CH 3 CN:H 2 O over 10 min.

    Article Snippet: The compound was resolved using Beckman Coulter C18 reversed-phase Ultrasphere analytical column (5 µm, 4.6 mm × 25 cm) at 1 mL/min using the following conditions: 100% H2 O for 5 min followed by a gradient from that point to 1:9 CH3 CN:H2 O over 10 min. As a reference, the cold (natural) Cu-SB-TE1A1P complex was co-injected under identical conditions.

    Techniques: High Performance Liquid Chromatography

    Purification of Kbot21 from scorpion Buthus occitanus tunetanus venom. (A) Chromatography of fraction BotG50 on semi preparative C8 reverse phase HPLC column. (B) Chromatography of fraction B1on C18-RP-HPLC, B’1 was collected at 29min. (C) Kbot21 was purified from the fraction B’1 by C18-RP-HPLC. It is collected at 21min.

    Journal: PLoS ONE

    Article Title: Characterization of Kbot21 Reveals Novel Side Chain Interactions of Scorpion Toxins Inhibiting Voltage-Gated Potassium Channels

    doi: 10.1371/journal.pone.0137611

    Figure Lengend Snippet: Purification of Kbot21 from scorpion Buthus occitanus tunetanus venom. (A) Chromatography of fraction BotG50 on semi preparative C8 reverse phase HPLC column. (B) Chromatography of fraction B1on C18-RP-HPLC, B’1 was collected at 29min. (C) Kbot21 was purified from the fraction B’1 by C18-RP-HPLC. It is collected at 21min.

    Article Snippet: HPLC purification of the non-retained fraction was performed using an analytical C18 reversed-phase HPLC column (4.6mm x 250 mm, 5 microns Beckman).

    Techniques: Purification, Chromatography, High Performance Liquid Chromatography

    Experimental design to evaluate polycyclic aromatic hydrocarbon ( PAH ) effects on cell populations in bone marrow (BM), spleen, and thymus . (A) The scheme for development of cell lineages from BM hematopoietic stem cells (HSC). Standard abbreviations are used for progenitors (long‐term HSC, LT‐HSC; short‐term HSC, ST‐HSC; common lymphoid progenitor, CLP; common myeloid progenitor, CMP; megakaryocyte‐erythroid progenitor, MEP; granulocyte‐macrophage progenitor, GMP) as defined in the Introduction. B220+ cells are B‐lymphocytes that migrate to the spleen for further maturation. CLP T are lymphoid progenitors modified for export to the thymus as circulating thymic progenitors (CTP). (B) Time‐dependent clearance of circulating dimethylbenz(a)anthracene ( DMBA), administered to wild type (WT) mice by Intraperitoneal (IP) injection or oral gavage (50 mg/kg in olive oil). Blood was collected by cardiac puncture and DMBA concentration measured by HPLC. The results represent the average of 2–3 experiments with n = 3–4 mice per assay.

    Journal: Pharmacology Research & Perspectives

    Article Title: Cyp1b1‐mediated suppression of lymphoid progenitors in bone marrow by polycyclic aromatic hydrocarbons coordinately impacts spleen and thymus: a selective role for the Ah Receptor. Cyp1b1‐mediated suppression of lymphoid progenitors in bone marrow by polycyclic aromatic hydrocarbons coordinately impacts spleen and thymus: a selective role for the Ah Receptor

    doi: 10.1002/prp2.245

    Figure Lengend Snippet: Experimental design to evaluate polycyclic aromatic hydrocarbon ( PAH ) effects on cell populations in bone marrow (BM), spleen, and thymus . (A) The scheme for development of cell lineages from BM hematopoietic stem cells (HSC). Standard abbreviations are used for progenitors (long‐term HSC, LT‐HSC; short‐term HSC, ST‐HSC; common lymphoid progenitor, CLP; common myeloid progenitor, CMP; megakaryocyte‐erythroid progenitor, MEP; granulocyte‐macrophage progenitor, GMP) as defined in the Introduction. B220+ cells are B‐lymphocytes that migrate to the spleen for further maturation. CLP T are lymphoid progenitors modified for export to the thymus as circulating thymic progenitors (CTP). (B) Time‐dependent clearance of circulating dimethylbenz(a)anthracene ( DMBA), administered to wild type (WT) mice by Intraperitoneal (IP) injection or oral gavage (50 mg/kg in olive oil). Blood was collected by cardiac puncture and DMBA concentration measured by HPLC. The results represent the average of 2–3 experiments with n = 3–4 mice per assay.

    Article Snippet: Residual PAH was extracted (2:1, acetone:acetonitrile) and separated by reverse‐phase HPLC (Beckman C18 Ultrasphere column), using a Waters 2695 Separation module interfaced to a Waters 470 scanning Fluorescence Detector, incorporating a 50–100% methanol gradient over a 30 min period.

    Techniques: Modification, Mouse Assay, Injection, Concentration Assay, High Performance Liquid Chromatography

    Representative HPLC chromatograms from in vitro incubations with pooled hepatic microsomal fractions from ellipticine-pretreated WT mice with ellipticine (ELLI) and either NADPH or NADH as cofactor. M1: 9-hydroxyellipticine; M2: 12-hydroxyellipticine; M3: 13-hydroxyellipticine. Phenacetin (PA) was used as internal standard.

    Journal: Toxicology and Applied Pharmacology

    Article Title: Application of hepatic cytochrome b5/P450 reductase null (HBRN) mice to study the role of cytochrome b5 in the cytochrome P450-mediated bioactivation of the anticancer drug ellipticine

    doi: 10.1016/j.taap.2019.01.020

    Figure Lengend Snippet: Representative HPLC chromatograms from in vitro incubations with pooled hepatic microsomal fractions from ellipticine-pretreated WT mice with ellipticine (ELLI) and either NADPH or NADH as cofactor. M1: 9-hydroxyellipticine; M2: 12-hydroxyellipticine; M3: 13-hydroxyellipticine. Phenacetin (PA) was used as internal standard.

    Article Snippet: Ellipticine metabolites were extracted twice with ethyl acetate (1 ml), solvent evaporated to dryness, residues dissolved in 25 μl methanol and ellipticine metabolites separated by high performance liquid chromatography (HPLC) (5 μm Ultrasphere ODS Beckman, 4.6 mm × 250 mm preceded by a C18 guard column).

    Techniques: High Performance Liquid Chromatography, In Vitro, Mouse Assay