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Bio-Rad c1000 touch thermal cycler
C1000 Touch Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c1000 touch thermal cycler/product/Bio-Rad
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
c1000 touch thermal cycler - by Bioz Stars, 2021-04
99/100 stars

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Polymerase Chain Reaction:

Article Title: Versatile protein tagging in cells with split fluorescent protein
Article Snippet: To optimize PCR annealing temperatures and characterize detection efficiencies, we mixed Bio-Rad ddPCR Supermix for Probes (12.5 μl), FAM- and HEX probe and primer premixture (1.25 μl each), and mixture of a genomic DNA (100 ng) and a knock-in allele plasmid (0.5 pg or series of diluted plasmid) in 25 μl total volume. .. Droplet generation, PCR reaction and droplets read were performed by a QX100 Droplet Generator, a C1000 Thermal Cycler and a QX100 Droplet Reader (Bio-Rad), respectively according to the instructions from the manufacturer. .. Droplets were analysed by QuantSoft software (Bio-Rad).

Incubation:

Article Title: pH Dependence of the Stress Regulator DksA
Article Snippet: DksA (50 μM) was mixed with 5X SYPRO Orange (the stock concentration is not disclosed by the manufacturer) in 50 mM HEPES buffer at pH 6, 7 and 8 with 100 mM NaCl. .. The 25 μl reaction was incubated in a C1000 Thermal Cycler (Bio-Rad, Hercules, CA) for 25 minutes at 25°C followed by a gradual increase (1°C/30 sec) in temperature; with fluorescence intensity recorded every 1°. .. The unfolding temperature (Tu) is defined as the temperature at which the fluorescence increase reaches the half-maximum, and was estimated separately from three independent experiments and then averaged.

Size-exclusion Chromatography:

Article Title: pH Dependence of the Stress Regulator DksA
Article Snippet: DksA (50 μM) was mixed with 5X SYPRO Orange (the stock concentration is not disclosed by the manufacturer) in 50 mM HEPES buffer at pH 6, 7 and 8 with 100 mM NaCl. .. The 25 μl reaction was incubated in a C1000 Thermal Cycler (Bio-Rad, Hercules, CA) for 25 minutes at 25°C followed by a gradual increase (1°C/30 sec) in temperature; with fluorescence intensity recorded every 1°. .. The unfolding temperature (Tu) is defined as the temperature at which the fluorescence increase reaches the half-maximum, and was estimated separately from three independent experiments and then averaged.

Fluorescence:

Article Title: pH Dependence of the Stress Regulator DksA
Article Snippet: DksA (50 μM) was mixed with 5X SYPRO Orange (the stock concentration is not disclosed by the manufacturer) in 50 mM HEPES buffer at pH 6, 7 and 8 with 100 mM NaCl. .. The 25 μl reaction was incubated in a C1000 Thermal Cycler (Bio-Rad, Hercules, CA) for 25 minutes at 25°C followed by a gradual increase (1°C/30 sec) in temperature; with fluorescence intensity recorded every 1°. .. The unfolding temperature (Tu) is defined as the temperature at which the fluorescence increase reaches the half-maximum, and was estimated separately from three independent experiments and then averaged.

Real-time Polymerase Chain Reaction:

Article Title: The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer
Article Snippet: .. Quantitative real-time PCR was performed for CTSK and GAPDH (housekeeping gene) using a C1000 Touch Thermal Cycler CFX96TM Real-Time System (Bio-Rad, Shanghai, China) per the Universal SYBR Green qPCR Supermix (UE, Suzhou, China) instructions. .. Real-time PCR was triplicated for each cDNA sample.

Article Title: Type II collagen and glycosaminoglycan expression induction in primary human chondrocyte by TGF-β1
Article Snippet: Reverse transcriptase-polymerase chain reaction (RT-PCR) was performed with a SuperScript II reverse transcriptase (Invitrogen by Life Technologies, Grand Island, NY, USA) and random primer mix (NEB, Ipswich, MA, USA). .. For qPCR, C1000 thermal cycler system with CFX96 real-time PCR detection systems (Bio-Rad, Hercules, CA, USA) and IQTM SYPR® Green supermix (Bio-Rad, Hercules, CA, USA) were used with the following primers: type II collagen (forward, 5'-CCCTGAGTGGAAGAGTGGAG-3', reverse, 5'-GAGGCGTGAGGTCTTCTGTG-3'), type I collagen (forward, 5'-CGATGGCTGCACGAGTCACAC-3', reverse, 5'-CAGGTTGGGATGGAGGGAGTTTAC-3'), GAPDH (forward, 5'- TCGACAGTCAGCCGCATCTTCTTT-3', reverse, 5'-ACCAAATCCGTTGACTCCGACCTT-3'). ..

Article Title: Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase
Article Snippet: .. Ten nanograms of DNA was used for subsequent qPCR reactions using a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad, 1855195). .. Baselines were subtracted using the baseline subtraction curve fit analysis mode and thresholds were automatically calculated using the Bio-Rad CFX Manager software version 2.1.

Article Title: Identification of TBK1 complexes required for the phosphorylation of IRF3 and the production of interferon β
Article Snippet: Polymerase chain reaction (PCR) mixes were assembled using the SsoFast™ EvaGreen® Supermix (Bio-Rad). .. Reactions were performed with the SYBR Green (plus melting curve analysis) programme on the C1000 thermal cycler quantitative PCR system (Bio-Rad). .. The concentration of IFNβ released into the cell culture medium was determined by using the LegendMax mouse IFNβ ELISA kit (Biolegend).

SYBR Green Assay:

Article Title: The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer
Article Snippet: .. Quantitative real-time PCR was performed for CTSK and GAPDH (housekeeping gene) using a C1000 Touch Thermal Cycler CFX96TM Real-Time System (Bio-Rad, Shanghai, China) per the Universal SYBR Green qPCR Supermix (UE, Suzhou, China) instructions. .. Real-time PCR was triplicated for each cDNA sample.

Article Title: Identification of TBK1 complexes required for the phosphorylation of IRF3 and the production of interferon β
Article Snippet: Polymerase chain reaction (PCR) mixes were assembled using the SsoFast™ EvaGreen® Supermix (Bio-Rad). .. Reactions were performed with the SYBR Green (plus melting curve analysis) programme on the C1000 thermal cycler quantitative PCR system (Bio-Rad). .. The concentration of IFNβ released into the cell culture medium was determined by using the LegendMax mouse IFNβ ELISA kit (Biolegend).

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  • 99
    Bio-Rad benchtop thermal cycler
    Nucleic acid amplification using cavity PCR. a) Results from 2% agarose gel demonstrating a clear trend of increased PCR byproduct with an increase in PCR cycle numbers. b) Image of 2% agarose gel with different heights of cavity PCR chamber. For the comparison, the same volume (2 μL) of PCR byproduct was used for gel electrophoresis. c) Summary of total amplification time for the <t>benchtop</t> and cavity PCR. The reaction time for the benchtop is based on the cycling protocol recommended by the manufacturer. A 70%–85% reduction in total reaction time can be achieved. d) Results from 2% agarose gel demonstrating a trend in band intensity with different concentrations of initial c‐MET cDNA. The lowest concentration of template DNA is 10 −8 ng μL −1 (two copies per μL). e) Results from 2% agarose gel showing the amplification of c‐MET cDNA prepared from NSCLC cell for the heterogeneous biological sample. (Standard: c‐MET cDNA purchased from Sino Biological Inc.) f) Success rate (%) of the cavity PCR with different concentrations of c‐MET cDNA ( n = 7 for 10 −5 , 10 −6 , 10 −7 ng μL −1 , n = 19 for 10 −8 ng μL −1 ).
    Benchtop Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/benchtop thermal cycler/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    benchtop thermal cycler - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    86
    Bio-Rad c1000 touch thermal cycler with 96 deep well reaction module
    Nucleic acid amplification using cavity PCR. a) Results from 2% agarose gel demonstrating a clear trend of increased PCR byproduct with an increase in PCR cycle numbers. b) Image of 2% agarose gel with different heights of cavity PCR chamber. For the comparison, the same volume (2 μL) of PCR byproduct was used for gel electrophoresis. c) Summary of total amplification time for the <t>benchtop</t> and cavity PCR. The reaction time for the benchtop is based on the cycling protocol recommended by the manufacturer. A 70%–85% reduction in total reaction time can be achieved. d) Results from 2% agarose gel demonstrating a trend in band intensity with different concentrations of initial c‐MET cDNA. The lowest concentration of template DNA is 10 −8 ng μL −1 (two copies per μL). e) Results from 2% agarose gel showing the amplification of c‐MET cDNA prepared from NSCLC cell for the heterogeneous biological sample. (Standard: c‐MET cDNA purchased from Sino Biological Inc.) f) Success rate (%) of the cavity PCR with different concentrations of c‐MET cDNA ( n = 7 for 10 −5 , 10 −6 , 10 −7 ng μL −1 , n = 19 for 10 −8 ng μL −1 ).
    C1000 Touch Thermal Cycler With 96 Deep Well Reaction Module, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c1000 touch thermal cycler with 96 deep well reaction module/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c1000 touch thermal cycler with 96 deep well reaction module - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Bio-Rad c1000 touch thermal cycler
    Nucleic acid amplification using cavity PCR. a) Results from 2% agarose gel demonstrating a clear trend of increased PCR byproduct with an increase in PCR cycle numbers. b) Image of 2% agarose gel with different heights of cavity PCR chamber. For the comparison, the same volume (2 μL) of PCR byproduct was used for gel electrophoresis. c) Summary of total amplification time for the <t>benchtop</t> and cavity PCR. The reaction time for the benchtop is based on the cycling protocol recommended by the manufacturer. A 70%–85% reduction in total reaction time can be achieved. d) Results from 2% agarose gel demonstrating a trend in band intensity with different concentrations of initial c‐MET cDNA. The lowest concentration of template DNA is 10 −8 ng μL −1 (two copies per μL). e) Results from 2% agarose gel showing the amplification of c‐MET cDNA prepared from NSCLC cell for the heterogeneous biological sample. (Standard: c‐MET cDNA purchased from Sino Biological Inc.) f) Success rate (%) of the cavity PCR with different concentrations of c‐MET cDNA ( n = 7 for 10 −5 , 10 −6 , 10 −7 ng μL −1 , n = 19 for 10 −8 ng μL −1 ).
    C1000 Touch Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c1000 touch thermal cycler/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c1000 touch thermal cycler - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Nucleic acid amplification using cavity PCR. a) Results from 2% agarose gel demonstrating a clear trend of increased PCR byproduct with an increase in PCR cycle numbers. b) Image of 2% agarose gel with different heights of cavity PCR chamber. For the comparison, the same volume (2 μL) of PCR byproduct was used for gel electrophoresis. c) Summary of total amplification time for the benchtop and cavity PCR. The reaction time for the benchtop is based on the cycling protocol recommended by the manufacturer. A 70%–85% reduction in total reaction time can be achieved. d) Results from 2% agarose gel demonstrating a trend in band intensity with different concentrations of initial c‐MET cDNA. The lowest concentration of template DNA is 10 −8 ng μL −1 (two copies per μL). e) Results from 2% agarose gel showing the amplification of c‐MET cDNA prepared from NSCLC cell for the heterogeneous biological sample. (Standard: c‐MET cDNA purchased from Sino Biological Inc.) f) Success rate (%) of the cavity PCR with different concentrations of c‐MET cDNA ( n = 7 for 10 −5 , 10 −6 , 10 −7 ng μL −1 , n = 19 for 10 −8 ng μL −1 ).

    Journal: Advanced Healthcare Materials

    Article Title: Rapid Optical Cavity PCR

    doi: 10.1002/adhm.201500708

    Figure Lengend Snippet: Nucleic acid amplification using cavity PCR. a) Results from 2% agarose gel demonstrating a clear trend of increased PCR byproduct with an increase in PCR cycle numbers. b) Image of 2% agarose gel with different heights of cavity PCR chamber. For the comparison, the same volume (2 μL) of PCR byproduct was used for gel electrophoresis. c) Summary of total amplification time for the benchtop and cavity PCR. The reaction time for the benchtop is based on the cycling protocol recommended by the manufacturer. A 70%–85% reduction in total reaction time can be achieved. d) Results from 2% agarose gel demonstrating a trend in band intensity with different concentrations of initial c‐MET cDNA. The lowest concentration of template DNA is 10 −8 ng μL −1 (two copies per μL). e) Results from 2% agarose gel showing the amplification of c‐MET cDNA prepared from NSCLC cell for the heterogeneous biological sample. (Standard: c‐MET cDNA purchased from Sino Biological Inc.) f) Success rate (%) of the cavity PCR with different concentrations of c‐MET cDNA ( n = 7 for 10 −5 , 10 −6 , 10 −7 ng μL −1 , n = 19 for 10 −8 ng μL −1 ).

    Article Snippet: Figure a shows an image of the 2% agarose gel from the benchtop thermal cycler (Bio‐Rad C1000 thermal cycler with CFX96 real‐time PCR detection system) and the cavity PCR (750 μm thick PCR chamber) with different cycle numbers from 95 °C to 68 °C.

    Techniques: Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Nucleic Acid Electrophoresis, Concentration Assay