c1000 touch thermal cycler  (bio-rad)

 
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    bio-rad c1000 touch thermal cycler
    C1000 Touch Thermal Cycler, supplied by bio-rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c1000 touch thermal cycler/product/bio-rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c1000 touch thermal cycler - by Bioz Stars, 2021-03
    86/100 stars

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    Quantitative RT-PCR:

    Article Title: Nuclear Factor κB Activation in a Type V Pityriasis Rubra Pilaris Patient Harboring Multiple CARD14 Variants
    Article Snippet: Peripheral blood mononuclear cells and keratinocytes were harvested using TRIzol® Reagent (Invitrogen Corp., Carlsbad, CA, USA), following the manufacturer’s instructions. cDNA was synthesized from 1 µg total RNA using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA). .. Real-time RT-PCR experiments were carried out with the Universal Probe Library system (Roche Diagnostics) using a C1000 Touch Thermal Cycler (Bio-Rad Laboratories). ..

    Purification:

    Article Title: Consensus designs and thermal stability determinants of a human glutamate transporter
    Article Snippet: The eluted eGFP-transporter fusion was digested with PreScission protease overnight at 4°C, concentrated to 100 μL using 100 kDa cutoff membranes (Millipore), ultra-centrifuged for 20 min at 86,900 g, and finally applied to a Superose 6 5/150 gel filtration column equilibrated with 50 mM HEPES/Tris-base, 200 mM NaCl, pH 7.4, 1 mM L-Asp, 0.5 mM TCEP, 0.0632% DDS, 0.01264% CHS and 5% glycerol. .. To evaluate and compare the stability of the EAAT1 consensus designs by HDX-MS, the purified proteins were heated at a single pre-pulse temperature for 20 min in a C1000 Touch Thermal Cycler (BioRad), ultra-centrifuged (86,900 g, 20 min) to clear the solution, and further equilibrated for 1 hr at 20°C before deuterium labeling. .. Deuterium exchange was initiated by adding 40 µL of D2 O buffer (50 mM HEPES, pD 7.4, 200 mM NaCl, 1 mM L-Asp, 5% glycerol, 0.0632% DDS, 0.01264% CHS, 0.5 mM TCEP) to 10 µL of EAAT1 protein solution at ~5 µM (monomer concentration).

    Labeling:

    Article Title: Consensus designs and thermal stability determinants of a human glutamate transporter
    Article Snippet: The eluted eGFP-transporter fusion was digested with PreScission protease overnight at 4°C, concentrated to 100 μL using 100 kDa cutoff membranes (Millipore), ultra-centrifuged for 20 min at 86,900 g, and finally applied to a Superose 6 5/150 gel filtration column equilibrated with 50 mM HEPES/Tris-base, 200 mM NaCl, pH 7.4, 1 mM L-Asp, 0.5 mM TCEP, 0.0632% DDS, 0.01264% CHS and 5% glycerol. .. To evaluate and compare the stability of the EAAT1 consensus designs by HDX-MS, the purified proteins were heated at a single pre-pulse temperature for 20 min in a C1000 Touch Thermal Cycler (BioRad), ultra-centrifuged (86,900 g, 20 min) to clear the solution, and further equilibrated for 1 hr at 20°C before deuterium labeling. .. Deuterium exchange was initiated by adding 40 µL of D2 O buffer (50 mM HEPES, pD 7.4, 200 mM NaCl, 1 mM L-Asp, 5% glycerol, 0.0632% DDS, 0.01264% CHS, 0.5 mM TCEP) to 10 µL of EAAT1 protein solution at ~5 µM (monomer concentration).

    Amplification:

    Article Title: Preliminary Studies on Immune Response and Viral Pathogenesis of Zika Virus in Rhesus Macaques
    Article Snippet: Plates were sealed with the PX1 PCR Plate Sealer (Bio-Rad, Hercules, CA, USA) prior to PCR. .. Target DNA was then amplified with the C1000 Touch Thermal Cycler (Bio-Rad, Hercules, CA, USA) using the following conditions: 1 cycle 48 °C for 1 h, 1 cycle 95 °C for 10 min, 40 cycles 95 °C for 30 s and 60 °C for 1 min, and 1 cycle 98 °C for 10 min. After amplification, the plate was read on a QX200 Droplet Reader (Bio-Rad, Hercules, CA, USA) to determine the number of PCR-positive droplets vs. PCR-negative droplets in the original sample. .. Data acquisition and quantification was performed using QuantaSoft Software (Bio-Rad, Hercules CA, USA).

    Article Title: Description of a new deep-water dogfish shark from Hawaii, with comments on the Squalusmitsukurii species complex in the West Pacific
    Article Snippet: PCR reactions consisting of 7 μL BioMix Red from Bioline (London, UK) at the recommended concentration, 1 μL (3 μg) template DNA, and 1 μL (1.0 μM) each primer (10 μL total PCR volume). .. PCR amplification on a C1000 Touch Thermal Cycler (Bio-Rad; Hercules, California) consisted of an initial denaturation at 95 °C for 4 minutes followed by 36 cycles of 1 min at 95 °C, followed by 30s at 58 °C, and 30s at 72 °C with a final extension at 72 °C for 20 minutes. ..

    Polymerase Chain Reaction:

    Article Title: Preliminary Studies on Immune Response and Viral Pathogenesis of Zika Virus in Rhesus Macaques
    Article Snippet: Plates were sealed with the PX1 PCR Plate Sealer (Bio-Rad, Hercules, CA, USA) prior to PCR. .. Target DNA was then amplified with the C1000 Touch Thermal Cycler (Bio-Rad, Hercules, CA, USA) using the following conditions: 1 cycle 48 °C for 1 h, 1 cycle 95 °C for 10 min, 40 cycles 95 °C for 30 s and 60 °C for 1 min, and 1 cycle 98 °C for 10 min. After amplification, the plate was read on a QX200 Droplet Reader (Bio-Rad, Hercules, CA, USA) to determine the number of PCR-positive droplets vs. PCR-negative droplets in the original sample. .. Data acquisition and quantification was performed using QuantaSoft Software (Bio-Rad, Hercules CA, USA).

    Article Title: Description of a new deep-water dogfish shark from Hawaii, with comments on the Squalusmitsukurii species complex in the West Pacific
    Article Snippet: PCR reactions consisting of 7 μL BioMix Red from Bioline (London, UK) at the recommended concentration, 1 μL (3 μg) template DNA, and 1 μL (1.0 μM) each primer (10 μL total PCR volume). .. PCR amplification on a C1000 Touch Thermal Cycler (Bio-Rad; Hercules, California) consisted of an initial denaturation at 95 °C for 4 minutes followed by 36 cycles of 1 min at 95 °C, followed by 30s at 58 °C, and 30s at 72 °C with a final extension at 72 °C for 20 minutes. ..

    Spectrophotometry:

    Article Title: Respiratory Syncytial Virus Infection in Mice and Detection of Viral Genomes in the Lung Using RT-qPCR
    Article Snippet: Eppendorf centrifuge 5415R (Eppendorf AG, catalog number: 22636570) Note: This product has been discontinued by Eppendorf AG. .. NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, catalog number: ND-1000) BioRad C1000 thermal cycler (Bio-Rad Laboratories, catalog number: 1851148EDU) MP Fastprep-24 homogenizer (MP Biomedicals, catalog number: 116004500) .. Note: This section describes the infection of Balb/C mice with RSV using the intranasal route.

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    • benchtop thermal cycler  (Bio-Rad)
    99
    Bio-Rad benchtop thermal cycler
    Nucleic acid amplification using cavity PCR. a) Results from 2% agarose gel demonstrating a clear trend of increased PCR byproduct with an increase in PCR cycle numbers. b) Image of 2% agarose gel with different heights of cavity PCR chamber. For the comparison, the same volume (2 μL) of PCR byproduct was used for gel electrophoresis. c) Summary of total amplification time for the <t>benchtop</t> and cavity PCR. The reaction time for the benchtop is based on the cycling protocol recommended by the manufacturer. A 70%–85% reduction in total reaction time can be achieved. d) Results from 2% agarose gel demonstrating a trend in band intensity with different concentrations of initial c‐MET cDNA. The lowest concentration of template DNA is 10 −8 ng μL −1 (two copies per μL). e) Results from 2% agarose gel showing the amplification of c‐MET cDNA prepared from NSCLC cell for the heterogeneous biological sample. (Standard: c‐MET cDNA purchased from Sino Biological Inc.) f) Success rate (%) of the cavity PCR with different concentrations of c‐MET cDNA ( n = 7 for 10 −5 , 10 −6 , 10 −7 ng μL −1 , n = 19 for 10 −8 ng μL −1 ).
    Benchtop Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/benchtop thermal cycler/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    benchtop thermal cycler - by Bioz Stars, 2021-03
    99/100 stars
    		  Buy from Supplier
    c1000 touch cfx96 real time system thermal cycler  (Bio-Rad)
    86
    Bio-Rad c1000 touch cfx96 real time system thermal cycler
    Nucleic acid amplification using cavity PCR. a) Results from 2% agarose gel demonstrating a clear trend of increased PCR byproduct with an increase in PCR cycle numbers. b) Image of 2% agarose gel with different heights of cavity PCR chamber. For the comparison, the same volume (2 μL) of PCR byproduct was used for gel electrophoresis. c) Summary of total amplification time for the <t>benchtop</t> and cavity PCR. The reaction time for the benchtop is based on the cycling protocol recommended by the manufacturer. A 70%–85% reduction in total reaction time can be achieved. d) Results from 2% agarose gel demonstrating a trend in band intensity with different concentrations of initial c‐MET cDNA. The lowest concentration of template DNA is 10 −8 ng μL −1 (two copies per μL). e) Results from 2% agarose gel showing the amplification of c‐MET cDNA prepared from NSCLC cell for the heterogeneous biological sample. (Standard: c‐MET cDNA purchased from Sino Biological Inc.) f) Success rate (%) of the cavity PCR with different concentrations of c‐MET cDNA ( n = 7 for 10 −5 , 10 −6 , 10 −7 ng μL −1 , n = 19 for 10 −8 ng μL −1 ).
    C1000 Touch Cfx96 Real Time System Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c1000 touch cfx96 real time system thermal cycler/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c1000 touch cfx96 real time system thermal cycler - by Bioz Stars, 2021-03
    86/100 stars
    		  Buy from Supplier
    cycler  (Bio-Rad)
    98
    Bio-Rad cycler
    Nucleic acid amplification using cavity PCR. a) Results from 2% agarose gel demonstrating a clear trend of increased PCR byproduct with an increase in PCR cycle numbers. b) Image of 2% agarose gel with different heights of cavity PCR chamber. For the comparison, the same volume (2 μL) of PCR byproduct was used for gel electrophoresis. c) Summary of total amplification time for the <t>benchtop</t> and cavity PCR. The reaction time for the benchtop is based on the cycling protocol recommended by the manufacturer. A 70%–85% reduction in total reaction time can be achieved. d) Results from 2% agarose gel demonstrating a trend in band intensity with different concentrations of initial c‐MET cDNA. The lowest concentration of template DNA is 10 −8 ng μL −1 (two copies per μL). e) Results from 2% agarose gel showing the amplification of c‐MET cDNA prepared from NSCLC cell for the heterogeneous biological sample. (Standard: c‐MET cDNA purchased from Sino Biological Inc.) f) Success rate (%) of the cavity PCR with different concentrations of c‐MET cDNA ( n = 7 for 10 −5 , 10 −6 , 10 −7 ng μL −1 , n = 19 for 10 −8 ng μL −1 ).
    Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cycler/product/Bio-Rad
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cycler - by Bioz Stars, 2021-03
    98/100 stars
    		  Buy from Supplier

    Image Search Results


    Nucleic acid amplification using cavity PCR. a) Results from 2% agarose gel demonstrating a clear trend of increased PCR byproduct with an increase in PCR cycle numbers. b) Image of 2% agarose gel with different heights of cavity PCR chamber. For the comparison, the same volume (2 μL) of PCR byproduct was used for gel electrophoresis. c) Summary of total amplification time for the benchtop and cavity PCR. The reaction time for the benchtop is based on the cycling protocol recommended by the manufacturer. A 70%–85% reduction in total reaction time can be achieved. d) Results from 2% agarose gel demonstrating a trend in band intensity with different concentrations of initial c‐MET cDNA. The lowest concentration of template DNA is 10 −8 ng μL −1 (two copies per μL). e) Results from 2% agarose gel showing the amplification of c‐MET cDNA prepared from NSCLC cell for the heterogeneous biological sample. (Standard: c‐MET cDNA purchased from Sino Biological Inc.) f) Success rate (%) of the cavity PCR with different concentrations of c‐MET cDNA ( n = 7 for 10 −5 , 10 −6 , 10 −7 ng μL −1 , n = 19 for 10 −8 ng μL −1 ).

    Journal: Advanced Healthcare Materials

    Article Title: Rapid Optical Cavity PCR

    doi: 10.1002/adhm.201500708

    Figure Lengend Snippet: Nucleic acid amplification using cavity PCR. a) Results from 2% agarose gel demonstrating a clear trend of increased PCR byproduct with an increase in PCR cycle numbers. b) Image of 2% agarose gel with different heights of cavity PCR chamber. For the comparison, the same volume (2 μL) of PCR byproduct was used for gel electrophoresis. c) Summary of total amplification time for the benchtop and cavity PCR. The reaction time for the benchtop is based on the cycling protocol recommended by the manufacturer. A 70%–85% reduction in total reaction time can be achieved. d) Results from 2% agarose gel demonstrating a trend in band intensity with different concentrations of initial c‐MET cDNA. The lowest concentration of template DNA is 10 −8 ng μL −1 (two copies per μL). e) Results from 2% agarose gel showing the amplification of c‐MET cDNA prepared from NSCLC cell for the heterogeneous biological sample. (Standard: c‐MET cDNA purchased from Sino Biological Inc.) f) Success rate (%) of the cavity PCR with different concentrations of c‐MET cDNA ( n = 7 for 10 −5 , 10 −6 , 10 −7 ng μL −1 , n = 19 for 10 −8 ng μL −1 ).

    Article Snippet: Figure a shows an image of the 2% agarose gel from the benchtop thermal cycler (Bio‐Rad C1000 thermal cycler with CFX96 real‐time PCR detection system) and the cavity PCR (750 μm thick PCR chamber) with different cycle numbers from 95 °C to 68 °C.

    Techniques: Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Nucleic Acid Electrophoresis, Concentration Assay