Structured Review

Bio-Rad c1000 thermal cycler
C1000 Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 656 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c1000 thermal cycler/product/Bio-Rad
Average 99 stars, based on 656 article reviews
Price from $9.99 to $1999.99
c1000 thermal cycler - by Bioz Stars, 2020-01
99/100 stars

Images

Related Articles

Amplification:

Article Title: Clonal evolution mechanisms in NT5C2 mutant relapsed acute lymphoblastic leukemia
Article Snippet: Amplicon sizes ranged from 75bp to 120bp. .. In line with the manufacturer’s instructions, an average of 7 million droplets were generated by the RainDrop Source instrument and emulsion PCR was performed using the C1000 Thermal Cycler (BioRad, Hercules, CA, USA).

Article Title: An extracellular network of Arabidopsis leucine-rich repeat receptor kinases
Article Snippet: Real-time PCR analyses were performed using a Roche LightCycler96 instrument (Roche Applied Science, Mannheim, Germany) and data analysed by the LightCycler 96 version 1.1 software or BioRad C1000 thermal cycler (BioRad). .. Relative gene expression levels were calculated using the 2-ΔΔCT method.

Article Title: Targeted Genome Editing in Human Repopulating Hematopoietic Stem Cells
Article Snippet: Genomic DNA from ZFN-treated CD34+ cells or their progeny harvested from transplanted mice was amplified using REPLI-g Mini Kit (QIAGEN) and the top ranking candidate off-target genomic loci from our previous study amplified by PCR generating amplicons of 389±20 bp surrounding the potential ZFN binding site. .. In order to build an equimolar library, PCR products were quantified with KAPA Library Quantification Kit for Illumina sequencing platforms (KAPABIOSYSTEMS) on C1000 Thermal Cycler (BIO-RAD) and sequenced on MiSeq Illumina Platform using MiSeq Reagent v.3 (Illumina).

Article Title: Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children
Article Snippet: Three G6PD variants (G6PD Mahidol c.487G > A; G6PD Kaiping c.1388G > A; and G6PD Mediterranean c.563C > T) previously reported in G6PD-deficient individuals in Myanmar were detected by real-time PCR with the BIO-RAD CFX 96 Real-Time System and C1000 Thermal Cycler (Bio-Rad, USA). .. Genotyping PCR, with melting curve analysis using dual-labelled, self-quenched probes, was performed using 25 μL reactions, containing 12.5 μL of iQ Multiplex Power Mix, 0.75 μL each of forward and reverse primers, 0.5 μl each of SNP and WT probes, 9 μL sterile distilled water, and 1 μL genomic DNA.

Mass Spectrometry:

Article Title: An extracellular network of Arabidopsis leucine-rich repeat receptor kinases
Article Snippet: Total RNA was isolated from 1-week-old seedlings grown on 1/2 MS plates using either the GeneMATRIX Universal RNA Purification Kit (EURx) or TRI Reagent (Sigma-Aldrich), followed by DNaseI treatment (Thermo Scientific). .. Real-time PCR analyses were performed using a Roche LightCycler96 instrument (Roche Applied Science, Mannheim, Germany) and data analysed by the LightCycler 96 version 1.1 software or BioRad C1000 thermal cycler (BioRad).

Article Title: Substrate Control in Stereoselective Lanthionine Biosynthesis
Article Snippet: All polymerase chain reactions (PCR) were carried out on a C1000™ thermal cycler (Bio-Rad). .. DNA sequencing was performed by ACGT, Inc. MALDI-TOF MS was carried out on a Bruker Daltonics UltrafleXtreme MALDI TOF/TOF instrument (Bruker).

Positive Control:

Article Title: Mesenchymal stem cells ameliorate inflammatory cytokine-induced impairment of AT-II cells through a keratinocyte growth factor-dependent PI3K/Akt/mTOR signaling pathway
Article Snippet: RT-PCR was conducted following the two step manufacturer's protocol of the PrimeScript™ RT-PCR kit (Takara Bio, Inc., Otsu, Japan) using the C1000 Thermal cycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA). .. First, the cDNA was prepared using the C1000 Thermal cycler, and the PCR reaction system was prepared in tubes with 1 µ l dNTP mixture (25 mM), 1 µ l Oligo (dt) primers (2.5 µ M), 1 µ g template RNA (or positive control RNA) and DEPC-treated water to a final volume of 10 µ l. Tubes were placed into the C1000 Thermal Cycler at 65°C for 5 min for denaturation and annealing. .. Subsequently, reverse transcription was conducted by preparing the following reagent mixture including 10 µ l reaction mixture from denaturation and annealing, 4 µ l 5X PrimeScript buffer, 0.5 µ l RNase inhititor, 0.5 µ l PrimeScript RTase and 5 µ l RNase DEPC-treated water to a final volume of 20 µ l reaction mixture.

Mouse Assay:

Article Title: Targeted Genome Editing in Human Repopulating Hematopoietic Stem Cells
Article Snippet: Genomic DNA from ZFN-treated CD34+ cells or their progeny harvested from transplanted mice was amplified using REPLI-g Mini Kit (QIAGEN) and the top ranking candidate off-target genomic loci from our previous study amplified by PCR generating amplicons of 389±20 bp surrounding the potential ZFN binding site. .. In order to build an equimolar library, PCR products were quantified with KAPA Library Quantification Kit for Illumina sequencing platforms (KAPABIOSYSTEMS) on C1000 Thermal Cycler (BIO-RAD) and sequenced on MiSeq Illumina Platform using MiSeq Reagent v.3 (Illumina).

Quantitative RT-PCR:

Article Title: Generation and selection of pluripotent stem cells for robust differentiation to insulin-secreting cells capable of reversing diabetes in rodents
Article Snippet: Purity and yield of RNA was confirmed using a NanoDrop 2000c spectrophotometer (ThermoFisher Scientific) and isolated RNA was stored at -80°C until RT-qPCR was performed. .. Reverse transcription to cDNA was performed using the iScript cDNA Synthesis Kit (BioRad #1708890) and a C1000 thermal cycler (BioRad). qPCR was performed using 50 ng cDNA in the SsoAdvanced Universal SYBR Green Supermix (BioRad #1725270), and a CFX96 real-time PCR system paired with a C1000 thermal cycler (BioRad).

SYBR Green Assay:

Article Title: Generation and selection of pluripotent stem cells for robust differentiation to insulin-secreting cells capable of reversing diabetes in rodents
Article Snippet: Purity and yield of RNA was confirmed using a NanoDrop 2000c spectrophotometer (ThermoFisher Scientific) and isolated RNA was stored at -80°C until RT-qPCR was performed. .. Reverse transcription to cDNA was performed using the iScript cDNA Synthesis Kit (BioRad #1708890) and a C1000 thermal cycler (BioRad). qPCR was performed using 50 ng cDNA in the SsoAdvanced Universal SYBR Green Supermix (BioRad #1725270), and a CFX96 real-time PCR system paired with a C1000 thermal cycler (BioRad). .. All primers were added at 400 nM and starting cDNA quantity was normalized across reactions with the housekeeping gene GAPDH.

Article Title: Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers
Article Snippet: RNA was isolated from transfected cells using the RNeasy Plus mini kit (Qiagen cat. #74136) and 500 ng of purified RNA was used as template for cDNA synthesis (Life Technologies, cat. #11754). .. Real-time PCR was performed using PerfeCTa SYBR Green FastMix (Quanta Biosciences, cat. #95072) and a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad). .. Baselines were subtracted using the baseline subtraction curve fit analysis mode and thresholds were automatically calculated using the Bio-Rad CFX Manager software version 2.1.

Microarray:

Article Title: Generation and selection of pluripotent stem cells for robust differentiation to insulin-secreting cells capable of reversing diabetes in rodents
Article Snippet: Reverse transcription to cDNA was performed using the iScript cDNA Synthesis Kit (BioRad #1708890) and a C1000 thermal cycler (BioRad). qPCR was performed using 50 ng cDNA in the SsoAdvanced Universal SYBR Green Supermix (BioRad #1725270), and a CFX96 real-time PCR system paired with a C1000 thermal cycler (BioRad). .. Reverse transcription to cDNA was performed using the iScript cDNA Synthesis Kit (BioRad #1708890) and a C1000 thermal cycler (BioRad). qPCR was performed using 50 ng cDNA in the SsoAdvanced Universal SYBR Green Supermix (BioRad #1725270), and a CFX96 real-time PCR system paired with a C1000 thermal cycler (BioRad).

Incubation:

Article Title: Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers
Article Snippet: Cross-links were reversed via overnight incubation at 65°C with sodium dodecyl sulfate, and DNA was purified using MinElute DNA purification columns (Qiagen). .. 10ng of DNA was used for subsequent qPCR reactions using a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad).

Article Title: In vitro isolation of small-molecule-binding aptamers with intrinsic dye-displacement functionality
Article Snippet: Polymerase chain reaction (PCR) was then performed using a BioRad C1000 thermal cycler. .. The double-stranded PCR product was immobilized on a micro-gravity column containing 200 μl of streptavidin-coated agarose resin.

Article Title: Engineering a Rugged Nanoscaffold To Enhance Plug-and-Display Vaccination
Article Snippet: % Conjugation was defined as 100 × [1 – (SpyCatcher-mi3 band after antigen incubation)/ (SpyCatcher-mi3 band in the absence of antigen)]. .. Temperature-Dependent Solubility Assay Thirty μL SpyCatcher-mi3 at 30 μM in 25 mM Tris·HCl, 150 mM NaCl, pH 8.5 was incubated at 25, 37, 55, 75, or 95 °C for 1 h and then cooled to 4 °C for 10 min on a C1000 thermal cycler (Bio-Rad). .. Following heating, aggregated proteins were pelleted by centrifugation at 16,900 g for 30 min at 4 °C.

Article Title: Engineering a Rugged Nanoscaffold To Enhance Plug-and-Display Vaccination
Article Snippet: % Conjugation was defined as 100 × [1 – (SpyCatcher-mi3 band after antigen incubation)/ (SpyCatcher-mi3 band in the absence of antigen)]. .. Thirty μL SpyCatcher-mi3 at 30 μM in 25 mM Tris·HCl, 150 mM NaCl, pH 8.5 was incubated at 25, 37, 55, 75, or 95 °C for 1 h and then cooled to 4 °C for 10 min on a C1000 thermal cycler (Bio-Rad). .. Following heating, aggregated proteins were pelleted by centrifugation at 16,900 g for 30 min at 4 °C.

Article Title: SUMOylation and the HSF1-Regulated Chaperone Network Converge to Promote Proteostasis in Response to Heat Shock
Article Snippet: Recombinant His6-FoxM1 was purified using TALON metal affinity resin and eluted from beads with 400 mM imidazole (pH 8). .. Recombinant MMS21 or FoxM1 was aliquoted into 11 or 16 samples of 50 μl each and incubated at increasing temperatures from 30–70°C for 5 min using gradient settings on a Bio-Rad C1000™ Thermal Cycler or a Veriti ™ 96-Well Thermal Cycler. .. For the experiments described in C, FoxM1 was aliquoted into samples of 50 μl and incubated for the indicated time periods at the indicated temperatures using a thermomixer (Eppendorf).

Activity Assay:

Article Title: Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children
Article Snippet: Three G6PD variants (G6PD Mahidol c.487G > A; G6PD Kaiping c.1388G > A; and G6PD Mediterranean c.563C > T) previously reported in G6PD-deficient individuals in Myanmar were detected by real-time PCR with the BIO-RAD CFX 96 Real-Time System and C1000 Thermal Cycler (Bio-Rad, USA). .. Genotyping PCR, with melting curve analysis using dual-labelled, self-quenched probes, was performed using 25 μL reactions, containing 12.5 μL of iQ Multiplex Power Mix, 0.75 μL each of forward and reverse primers, 0.5 μl each of SNP and WT probes, 9 μL sterile distilled water, and 1 μL genomic DNA.

Expressing:

Article Title: An extracellular network of Arabidopsis leucine-rich repeat receptor kinases
Article Snippet: Real-time PCR analyses were performed using a Roche LightCycler96 instrument (Roche Applied Science, Mannheim, Germany) and data analysed by the LightCycler 96 version 1.1 software or BioRad C1000 thermal cycler (BioRad). .. Real-time PCR analyses were performed using a Roche LightCycler96 instrument (Roche Applied Science, Mannheim, Germany) and data analysed by the LightCycler 96 version 1.1 software or BioRad C1000 thermal cycler (BioRad).

Article Title: Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers
Article Snippet: Real-time PCR was performed using PerfeCTa SYBR Green FastMix (Quanta Biosciences, cat. #95072) and a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad). .. Baselines were subtracted using the baseline subtraction curve fit analysis mode and thresholds were automatically calculated using the Bio-Rad CFX Manager software version 2.1.

Transfection:

Article Title: Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers
Article Snippet: RNA was isolated from transfected cells using the RNeasy Plus mini kit (Qiagen cat. #74136) and 500 ng of purified RNA was used as template for cDNA synthesis (Life Technologies, cat. #11754). .. Real-time PCR was performed using PerfeCTa SYBR Green FastMix (Quanta Biosciences, cat. #95072) and a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad).

Concentration Assay:

Article Title: Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers
Article Snippet: 10ng of DNA was used for subsequent qPCR reactions using a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad). .. 10ng of DNA was used for subsequent qPCR reactions using a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad).

Article Title: In vitro isolation of small-molecule-binding aptamers with intrinsic dye-displacement functionality
Article Snippet: Polymerase chain reaction (PCR) was then performed using a BioRad C1000 thermal cycler. .. Finally, the eluent was collected and neutralized with 0.2 M HCl in a 1.5 ml microcentrifugation tube and concentrated using a 3K molecular weight cut-off spin filter.

Solubility:

Article Title: Engineering a Rugged Nanoscaffold To Enhance Plug-and-Display Vaccination
Article Snippet: % Conjugation was defined as 100 × [1 – (SpyCatcher-mi3 band after antigen incubation)/ (SpyCatcher-mi3 band in the absence of antigen)]. .. Temperature-Dependent Solubility Assay Thirty μL SpyCatcher-mi3 at 30 μM in 25 mM Tris·HCl, 150 mM NaCl, pH 8.5 was incubated at 25, 37, 55, 75, or 95 °C for 1 h and then cooled to 4 °C for 10 min on a C1000 thermal cycler (Bio-Rad). .. Following heating, aggregated proteins were pelleted by centrifugation at 16,900 g for 30 min at 4 °C.

Article Title: Engineering a Rugged Nanoscaffold To Enhance Plug-and-Display Vaccination
Article Snippet: Paragraph title: Temperature-Dependent Solubility Assay ... Thirty μL SpyCatcher-mi3 at 30 μM in 25 mM Tris·HCl, 150 mM NaCl, pH 8.5 was incubated at 25, 37, 55, 75, or 95 °C for 1 h and then cooled to 4 °C for 10 min on a C1000 thermal cycler (Bio-Rad).

SDS Page:

Article Title: Engineering a Rugged Nanoscaffold To Enhance Plug-and-Display Vaccination
Article Snippet: Temperature-Dependent Solubility Assay Thirty μL SpyCatcher-mi3 at 30 μM in 25 mM Tris·HCl, 150 mM NaCl, pH 8.5 was incubated at 25, 37, 55, 75, or 95 °C for 1 h and then cooled to 4 °C for 10 min on a C1000 thermal cycler (Bio-Rad). .. Temperature-Dependent Solubility Assay Thirty μL SpyCatcher-mi3 at 30 μM in 25 mM Tris·HCl, 150 mM NaCl, pH 8.5 was incubated at 25, 37, 55, 75, or 95 °C for 1 h and then cooled to 4 °C for 10 min on a C1000 thermal cycler (Bio-Rad).

Article Title: Engineering a Rugged Nanoscaffold To Enhance Plug-and-Display Vaccination
Article Snippet: Thirty μL SpyCatcher-mi3 at 30 μM in 25 mM Tris·HCl, 150 mM NaCl, pH 8.5 was incubated at 25, 37, 55, 75, or 95 °C for 1 h and then cooled to 4 °C for 10 min on a C1000 thermal cycler (Bio-Rad). .. Thirty μL SpyCatcher-mi3 at 30 μM in 25 mM Tris·HCl, 150 mM NaCl, pH 8.5 was incubated at 25, 37, 55, 75, or 95 °C for 1 h and then cooled to 4 °C for 10 min on a C1000 thermal cycler (Bio-Rad).

Digital PCR:

Article Title: Clonal evolution mechanisms in NT5C2 mutant relapsed acute lymphoblastic leukemia
Article Snippet: The ddPCR reaction further contained 1X TaqMan Genotyping Master Mix (Applied Biosystems, Foster City, CA, USA), 1X dPCR droplet stabilizer (RainDance Techonologies), and 1X TaqMan primers and probes mix (Integrated DNA Technologies, Coralville, IA, USA). .. In line with the manufacturer’s instructions, an average of 7 million droplets were generated by the RainDrop Source instrument and emulsion PCR was performed using the C1000 Thermal Cycler (BioRad, Hercules, CA, USA).

Generated:

Article Title: Clonal evolution mechanisms in NT5C2 mutant relapsed acute lymphoblastic leukemia
Article Snippet: The ddPCR reaction further contained 1X TaqMan Genotyping Master Mix (Applied Biosystems, Foster City, CA, USA), 1X dPCR droplet stabilizer (RainDance Techonologies), and 1X TaqMan primers and probes mix (Integrated DNA Technologies, Coralville, IA, USA). .. In line with the manufacturer’s instructions, an average of 7 million droplets were generated by the RainDrop Source instrument and emulsion PCR was performed using the C1000 Thermal Cycler (BioRad, Hercules, CA, USA). .. Droplet fluorescence of the amplified product was detected by the RainDrop Sense instrument and data analysis was carried out using the RainDrop Analyst II Software (RainDance Techonolgies).

other:

Article Title: Engineering a Rugged Nanoscaffold To Enhance Plug-and-Display Vaccination
Article Snippet: Samples were adjusted to 0.125 mg/mL, before analyzing by DLS as above.

Tandem Mass Spectroscopy:

Article Title: Substrate Control in Stereoselective Lanthionine Biosynthesis
Article Snippet: All polymerase chain reactions (PCR) were carried out on a C1000™ thermal cycler (Bio-Rad). .. DNA sequencing was performed by ACGT, Inc. MALDI-TOF MS was carried out on a Bruker Daltonics UltrafleXtreme MALDI TOF/TOF instrument (Bruker).

Sequencing:

Article Title: Clonal evolution mechanisms in NT5C2 mutant relapsed acute lymphoblastic leukemia
Article Snippet: In line with the manufacturer’s instructions, an average of 7 million droplets were generated by the RainDrop Source instrument and emulsion PCR was performed using the C1000 Thermal Cycler (BioRad, Hercules, CA, USA). .. To determine the detection limit of the assays, we constructed dilution curves of patient tumor cells and cells from the REH cell line.

Article Title: Targeted Genome Editing in Human Repopulating Hematopoietic Stem Cells
Article Snippet: PCR products were purified using Agencourt AMPure XP beads (Beckman Coulter) and adaptors were added by TruSeq DNA LT Sample Prep Kit (Illumina). .. In order to build an equimolar library, PCR products were quantified with KAPA Library Quantification Kit for Illumina sequencing platforms (KAPABIOSYSTEMS) on C1000 Thermal Cycler (BIO-RAD) and sequenced on MiSeq Illumina Platform using MiSeq Reagent v.3 (Illumina). .. Raw paired-end reads were joined with Fastq-Join program from the EA-Utils NGS suite ( http://code.google.com/p/ea-utils/ ) and aligned to the specific genomic target sequences using Burrows-Wheeler Alignment Tool with maximal exact match version, BWA-MEM .

Software:

Article Title: An extracellular network of Arabidopsis leucine-rich repeat receptor kinases
Article Snippet: The cDNAs were used as a template for quantitative real-time PCR. .. Real-time PCR analyses were performed using a Roche LightCycler96 instrument (Roche Applied Science, Mannheim, Germany) and data analysed by the LightCycler 96 version 1.1 software or BioRad C1000 thermal cycler (BioRad). .. FastStart Essential DNA Green Master (Roche) or Maxima SYBR Green/ROX qPCR Master Mix (Thermo Scientific) were used according to manufacturer’s instructions.

Article Title: Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children
Article Snippet: Three G6PD variants (G6PD Mahidol c.487G > A; G6PD Kaiping c.1388G > A; and G6PD Mediterranean c.563C > T) previously reported in G6PD-deficient individuals in Myanmar were detected by real-time PCR with the BIO-RAD CFX 96 Real-Time System and C1000 Thermal Cycler (Bio-Rad, USA). .. The samples were run together with the plasmid control for each targeted mutation; three separate PCR reactions were run in every sample for the three different mutations.

Molecular Weight:

Article Title: In vitro isolation of small-molecule-binding aptamers with intrinsic dye-displacement functionality
Article Snippet: The combined eluted aptamers were concentrated using a 3K molecular weight cut-off spin filter (Millipore) and mixed with 1 μM forward primer ( , FP) and 1 μM biotinylated reverse primer ( , RP-bio) in 1 ml GoTaq Hot Start Master Mix (Promega). .. Polymerase chain reaction (PCR) was then performed using a BioRad C1000 thermal cycler.

Fluorescence:

Article Title: Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children
Article Snippet: Three G6PD variants (G6PD Mahidol c.487G > A; G6PD Kaiping c.1388G > A; and G6PD Mediterranean c.563C > T) previously reported in G6PD-deficient individuals in Myanmar were detected by real-time PCR with the BIO-RAD CFX 96 Real-Time System and C1000 Thermal Cycler (Bio-Rad, USA). .. The samples were run together with the plasmid control for each targeted mutation; three separate PCR reactions were run in every sample for the three different mutations.

Magnetic Beads:

Article Title: Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers
Article Snippet: H3K27ac enrichment was performed by incubation with 5 μg of Abcam ab4729 and 200 μl of sheep anti-rabbit IgG magnetic beads (Life Technologies 11203D) for 16 hrs at 4°. .. 10ng of DNA was used for subsequent qPCR reactions using a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad).

Mutagenesis:

Article Title: Clonal evolution mechanisms in NT5C2 mutant relapsed acute lymphoblastic leukemia
Article Snippet: Probes matching the wild type allele were labeled with VIC fluorescent reporter dye and probes matching the mutant allele were labeled with FAM dye. .. In line with the manufacturer’s instructions, an average of 7 million droplets were generated by the RainDrop Source instrument and emulsion PCR was performed using the C1000 Thermal Cycler (BioRad, Hercules, CA, USA).

Article Title: Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children
Article Snippet: Three G6PD variants (G6PD Mahidol c.487G > A; G6PD Kaiping c.1388G > A; and G6PD Mediterranean c.563C > T) previously reported in G6PD-deficient individuals in Myanmar were detected by real-time PCR with the BIO-RAD CFX 96 Real-Time System and C1000 Thermal Cycler (Bio-Rad, USA). .. The amplification conditions were 3 minutes at 95°C for iQ Multiplex Power Mix activity, followed by 40 cycles of 15 seconds at 95°C for denaturation, and 1 min at 70°C for annealing and extension for G6PD genotyping.

Isolation:

Article Title: Generation and selection of pluripotent stem cells for robust differentiation to insulin-secreting cells capable of reversing diabetes in rodents
Article Snippet: Purity and yield of RNA was confirmed using a NanoDrop 2000c spectrophotometer (ThermoFisher Scientific) and isolated RNA was stored at -80°C until RT-qPCR was performed. .. Reverse transcription to cDNA was performed using the iScript cDNA Synthesis Kit (BioRad #1708890) and a C1000 thermal cycler (BioRad). qPCR was performed using 50 ng cDNA in the SsoAdvanced Universal SYBR Green Supermix (BioRad #1725270), and a CFX96 real-time PCR system paired with a C1000 thermal cycler (BioRad).

Article Title: An extracellular network of Arabidopsis leucine-rich repeat receptor kinases
Article Snippet: Paragraph title: RNA isolation, cDNA synthesis and real-time PCR analysis ... Real-time PCR analyses were performed using a Roche LightCycler96 instrument (Roche Applied Science, Mannheim, Germany) and data analysed by the LightCycler 96 version 1.1 software or BioRad C1000 thermal cycler (BioRad).

Article Title: Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers
Article Snippet: RNA was isolated from transfected cells using the RNeasy Plus mini kit (Qiagen cat. #74136) and 500 ng of purified RNA was used as template for cDNA synthesis (Life Technologies, cat. #11754). .. Real-time PCR was performed using PerfeCTa SYBR Green FastMix (Quanta Biosciences, cat. #95072) and a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad).

Flow Cytometry:

Article Title: Clonal evolution mechanisms in NT5C2 mutant relapsed acute lymphoblastic leukemia
Article Snippet: In line with the manufacturer’s instructions, an average of 7 million droplets were generated by the RainDrop Source instrument and emulsion PCR was performed using the C1000 Thermal Cycler (BioRad, Hercules, CA, USA). .. The REH cell line was confirmed wild type after Sanger sequencing for the locations targeted in the ddPCR.

Labeling:

Article Title: Clonal evolution mechanisms in NT5C2 mutant relapsed acute lymphoblastic leukemia
Article Snippet: Probes matching the wild type allele were labeled with VIC fluorescent reporter dye and probes matching the mutant allele were labeled with FAM dye. .. In line with the manufacturer’s instructions, an average of 7 million droplets were generated by the RainDrop Source instrument and emulsion PCR was performed using the C1000 Thermal Cycler (BioRad, Hercules, CA, USA).

Size-exclusion Chromatography:

Article Title: An extracellular network of Arabidopsis leucine-rich repeat receptor kinases
Article Snippet: Real-time PCR analyses were performed using a Roche LightCycler96 instrument (Roche Applied Science, Mannheim, Germany) and data analysed by the LightCycler 96 version 1.1 software or BioRad C1000 thermal cycler (BioRad). .. Relative gene expression levels were calculated using the 2-ΔΔCT method.

Polymerase Chain Reaction:

Article Title: Clonal evolution mechanisms in NT5C2 mutant relapsed acute lymphoblastic leukemia
Article Snippet: The ddPCR reaction further contained 1X TaqMan Genotyping Master Mix (Applied Biosystems, Foster City, CA, USA), 1X dPCR droplet stabilizer (RainDance Techonologies), and 1X TaqMan primers and probes mix (Integrated DNA Technologies, Coralville, IA, USA). .. In line with the manufacturer’s instructions, an average of 7 million droplets were generated by the RainDrop Source instrument and emulsion PCR was performed using the C1000 Thermal Cycler (BioRad, Hercules, CA, USA). .. Droplet fluorescence of the amplified product was detected by the RainDrop Sense instrument and data analysis was carried out using the RainDrop Analyst II Software (RainDance Techonolgies).

Article Title: Mesenchymal stem cells ameliorate inflammatory cytokine-induced impairment of AT-II cells through a keratinocyte growth factor-dependent PI3K/Akt/mTOR signaling pathway
Article Snippet: RT-PCR was conducted following the two step manufacturer's protocol of the PrimeScript™ RT-PCR kit (Takara Bio, Inc., Otsu, Japan) using the C1000 Thermal cycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA). .. First, the cDNA was prepared using the C1000 Thermal cycler, and the PCR reaction system was prepared in tubes with 1 µ l dNTP mixture (25 mM), 1 µ l Oligo (dt) primers (2.5 µ M), 1 µ g template RNA (or positive control RNA) and DEPC-treated water to a final volume of 10 µ l. Tubes were placed into the C1000 Thermal Cycler at 65°C for 5 min for denaturation and annealing. .. Subsequently, reverse transcription was conducted by preparing the following reagent mixture including 10 µ l reaction mixture from denaturation and annealing, 4 µ l 5X PrimeScript buffer, 0.5 µ l RNase inhititor, 0.5 µ l PrimeScript RTase and 5 µ l RNase DEPC-treated water to a final volume of 20 µ l reaction mixture.

Article Title: Targeted Genome Editing in Human Repopulating Hematopoietic Stem Cells
Article Snippet: PCR products were purified using Agencourt AMPure XP beads (Beckman Coulter) and adaptors were added by TruSeq DNA LT Sample Prep Kit (Illumina). .. In order to build an equimolar library, PCR products were quantified with KAPA Library Quantification Kit for Illumina sequencing platforms (KAPABIOSYSTEMS) on C1000 Thermal Cycler (BIO-RAD) and sequenced on MiSeq Illumina Platform using MiSeq Reagent v.3 (Illumina). .. Raw paired-end reads were joined with Fastq-Join program from the EA-Utils NGS suite ( http://code.google.com/p/ea-utils/ ) and aligned to the specific genomic target sequences using Burrows-Wheeler Alignment Tool with maximal exact match version, BWA-MEM .

Article Title: In vitro isolation of small-molecule-binding aptamers with intrinsic dye-displacement functionality
Article Snippet: The combined eluted aptamers were concentrated using a 3K molecular weight cut-off spin filter (Millipore) and mixed with 1 μM forward primer ( , FP) and 1 μM biotinylated reverse primer ( , RP-bio) in 1 ml GoTaq Hot Start Master Mix (Promega). .. Polymerase chain reaction (PCR) was then performed using a BioRad C1000 thermal cycler. .. The double-stranded PCR product was immobilized on a micro-gravity column containing 200 μl of streptavidin-coated agarose resin.

Article Title: Substrate Control in Stereoselective Lanthionine Biosynthesis
Article Snippet: Structural information of substrate bound to the enzyme will be required to determine whether all calculated transition states can be attained in the context of the protein; at present no structures of LanM enzymes are available. .. All polymerase chain reactions (PCR) were carried out on a C1000™ thermal cycler (Bio-Rad). .. DNA sequencing was performed by ACGT, Inc. MALDI-TOF MS was carried out on a Bruker Daltonics UltrafleXtreme MALDI TOF/TOF instrument (Bruker).

Article Title: Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children
Article Snippet: Three G6PD variants (G6PD Mahidol c.487G > A; G6PD Kaiping c.1388G > A; and G6PD Mediterranean c.563C > T) previously reported in G6PD-deficient individuals in Myanmar were detected by real-time PCR with the BIO-RAD CFX 96 Real-Time System and C1000 Thermal Cycler (Bio-Rad, USA). .. The amplification conditions were 3 minutes at 95°C for iQ Multiplex Power Mix activity, followed by 40 cycles of 15 seconds at 95°C for denaturation, and 1 min at 70°C for annealing and extension for G6PD genotyping.

Article Title: Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers
Article Snippet: Paragraph title: Quantitative reverse-transcription PCR ... Real-time PCR was performed using PerfeCTa SYBR Green FastMix (Quanta Biosciences, cat. #95072) and a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad).

Binding Assay:

Article Title: Targeted Genome Editing in Human Repopulating Hematopoietic Stem Cells
Article Snippet: Genomic DNA from ZFN-treated CD34+ cells or their progeny harvested from transplanted mice was amplified using REPLI-g Mini Kit (QIAGEN) and the top ranking candidate off-target genomic loci from our previous study amplified by PCR generating amplicons of 389±20 bp surrounding the potential ZFN binding site. .. In order to build an equimolar library, PCR products were quantified with KAPA Library Quantification Kit for Illumina sequencing platforms (KAPABIOSYSTEMS) on C1000 Thermal Cycler (BIO-RAD) and sequenced on MiSeq Illumina Platform using MiSeq Reagent v.3 (Illumina).

Construct:

Article Title: Clonal evolution mechanisms in NT5C2 mutant relapsed acute lymphoblastic leukemia
Article Snippet: In line with the manufacturer’s instructions, an average of 7 million droplets were generated by the RainDrop Source instrument and emulsion PCR was performed using the C1000 Thermal Cycler (BioRad, Hercules, CA, USA). .. Droplet fluorescence of the amplified product was detected by the RainDrop Sense instrument and data analysis was carried out using the RainDrop Analyst II Software (RainDance Techonolgies).

IA:

Article Title: Clonal evolution mechanisms in NT5C2 mutant relapsed acute lymphoblastic leukemia
Article Snippet: The ddPCR reaction further contained 1X TaqMan Genotyping Master Mix (Applied Biosystems, Foster City, CA, USA), 1X dPCR droplet stabilizer (RainDance Techonologies), and 1X TaqMan primers and probes mix (Integrated DNA Technologies, Coralville, IA, USA). .. In line with the manufacturer’s instructions, an average of 7 million droplets were generated by the RainDrop Source instrument and emulsion PCR was performed using the C1000 Thermal Cycler (BioRad, Hercules, CA, USA).

Purification:

Article Title: An extracellular network of Arabidopsis leucine-rich repeat receptor kinases
Article Snippet: Total RNA was isolated from 1-week-old seedlings grown on 1/2 MS plates using either the GeneMATRIX Universal RNA Purification Kit (EURx) or TRI Reagent (Sigma-Aldrich), followed by DNaseI treatment (Thermo Scientific). .. Real-time PCR analyses were performed using a Roche LightCycler96 instrument (Roche Applied Science, Mannheim, Germany) and data analysed by the LightCycler 96 version 1.1 software or BioRad C1000 thermal cycler (BioRad).

Article Title: Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers
Article Snippet: Cross-links were reversed via overnight incubation at 65°C with sodium dodecyl sulfate, and DNA was purified using MinElute DNA purification columns (Qiagen). .. 10ng of DNA was used for subsequent qPCR reactions using a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad).

Article Title: Targeted Genome Editing in Human Repopulating Hematopoietic Stem Cells
Article Snippet: PCR products were purified using Agencourt AMPure XP beads (Beckman Coulter) and adaptors were added by TruSeq DNA LT Sample Prep Kit (Illumina). .. In order to build an equimolar library, PCR products were quantified with KAPA Library Quantification Kit for Illumina sequencing platforms (KAPABIOSYSTEMS) on C1000 Thermal Cycler (BIO-RAD) and sequenced on MiSeq Illumina Platform using MiSeq Reagent v.3 (Illumina).

Article Title: Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers
Article Snippet: RNA was isolated from transfected cells using the RNeasy Plus mini kit (Qiagen cat. #74136) and 500 ng of purified RNA was used as template for cDNA synthesis (Life Technologies, cat. #11754). .. Real-time PCR was performed using PerfeCTa SYBR Green FastMix (Quanta Biosciences, cat. #95072) and a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad).

Chromatin Immunoprecipitation:

Article Title: Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers
Article Snippet: Paragraph title: ChIP-qPCR ... 10ng of DNA was used for subsequent qPCR reactions using a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad).

Liquid Chromatography:

Article Title: Substrate Control in Stereoselective Lanthionine Biosynthesis
Article Snippet: All polymerase chain reactions (PCR) were carried out on a C1000™ thermal cycler (Bio-Rad). .. DNA sequencing was performed by ACGT, Inc. MALDI-TOF MS was carried out on a Bruker Daltonics UltrafleXtreme MALDI TOF/TOF instrument (Bruker).

Plasmid Preparation:

Article Title: Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children
Article Snippet: Three G6PD variants (G6PD Mahidol c.487G > A; G6PD Kaiping c.1388G > A; and G6PD Mediterranean c.563C > T) previously reported in G6PD-deficient individuals in Myanmar were detected by real-time PCR with the BIO-RAD CFX 96 Real-Time System and C1000 Thermal Cycler (Bio-Rad, USA). .. The amplification conditions were 3 minutes at 95°C for iQ Multiplex Power Mix activity, followed by 40 cycles of 15 seconds at 95°C for denaturation, and 1 min at 70°C for annealing and extension for G6PD genotyping.

TaqMan Assay:

Article Title: Clonal evolution mechanisms in NT5C2 mutant relapsed acute lymphoblastic leukemia
Article Snippet: Primers and probes for NT5C2 p.R39Q were designed through the Custom TaqMan® Assay Design Tool (CADT) (Life Technologies) with the support of RainDance Technologies. .. In line with the manufacturer’s instructions, an average of 7 million droplets were generated by the RainDrop Source instrument and emulsion PCR was performed using the C1000 Thermal Cycler (BioRad, Hercules, CA, USA).

Real-time Polymerase Chain Reaction:

Article Title: Generation and selection of pluripotent stem cells for robust differentiation to insulin-secreting cells capable of reversing diabetes in rodents
Article Snippet: Purity and yield of RNA was confirmed using a NanoDrop 2000c spectrophotometer (ThermoFisher Scientific) and isolated RNA was stored at -80°C until RT-qPCR was performed. .. Reverse transcription to cDNA was performed using the iScript cDNA Synthesis Kit (BioRad #1708890) and a C1000 thermal cycler (BioRad). qPCR was performed using 50 ng cDNA in the SsoAdvanced Universal SYBR Green Supermix (BioRad #1725270), and a CFX96 real-time PCR system paired with a C1000 thermal cycler (BioRad). .. All primers were added at 400 nM and starting cDNA quantity was normalized across reactions with the housekeeping gene GAPDH.

Article Title: An extracellular network of Arabidopsis leucine-rich repeat receptor kinases
Article Snippet: The cDNAs were used as a template for quantitative real-time PCR. .. Real-time PCR analyses were performed using a Roche LightCycler96 instrument (Roche Applied Science, Mannheim, Germany) and data analysed by the LightCycler 96 version 1.1 software or BioRad C1000 thermal cycler (BioRad). .. FastStart Essential DNA Green Master (Roche) or Maxima SYBR Green/ROX qPCR Master Mix (Thermo Scientific) were used according to manufacturer’s instructions.

Article Title: Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers
Article Snippet: Cross-links were reversed via overnight incubation at 65°C with sodium dodecyl sulfate, and DNA was purified using MinElute DNA purification columns (Qiagen). .. 10ng of DNA was used for subsequent qPCR reactions using a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad). .. Baselines were subtracted using the baseline subtraction curve fit analysis mode and thresholds were automatically calculated using the Bio-Rad CFX Manager software version 2.1.

Article Title: Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children
Article Snippet: DNA samples were extracted from 200 μL of whole blood using the QIAamp DNA Blood Mini Kit (Qiagen, Germany) according to the manufacturer’s recommendation. .. Three G6PD variants (G6PD Mahidol c.487G > A; G6PD Kaiping c.1388G > A; and G6PD Mediterranean c.563C > T) previously reported in G6PD-deficient individuals in Myanmar were detected by real-time PCR with the BIO-RAD CFX 96 Real-Time System and C1000 Thermal Cycler (Bio-Rad, USA). .. Genotyping PCR, with melting curve analysis using dual-labelled, self-quenched probes, was performed using 25 μL reactions, containing 12.5 μL of iQ Multiplex Power Mix, 0.75 μL each of forward and reverse primers, 0.5 μl each of SNP and WT probes, 9 μL sterile distilled water, and 1 μL genomic DNA.

Article Title: Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers
Article Snippet: RNA was isolated from transfected cells using the RNeasy Plus mini kit (Qiagen cat. #74136) and 500 ng of purified RNA was used as template for cDNA synthesis (Life Technologies, cat. #11754). .. Real-time PCR was performed using PerfeCTa SYBR Green FastMix (Quanta Biosciences, cat. #95072) and a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad). .. Baselines were subtracted using the baseline subtraction curve fit analysis mode and thresholds were automatically calculated using the Bio-Rad CFX Manager software version 2.1.

Multiplex Assay:

Article Title: Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children
Article Snippet: Three G6PD variants (G6PD Mahidol c.487G > A; G6PD Kaiping c.1388G > A; and G6PD Mediterranean c.563C > T) previously reported in G6PD-deficient individuals in Myanmar were detected by real-time PCR with the BIO-RAD CFX 96 Real-Time System and C1000 Thermal Cycler (Bio-Rad, USA). .. Genotyping PCR, with melting curve analysis using dual-labelled, self-quenched probes, was performed using 25 μL reactions, containing 12.5 μL of iQ Multiplex Power Mix, 0.75 μL each of forward and reverse primers, 0.5 μl each of SNP and WT probes, 9 μL sterile distilled water, and 1 μL genomic DNA.

Selection:

Article Title: In vitro isolation of small-molecule-binding aptamers with intrinsic dye-displacement functionality
Article Snippet: Target-bound aptamers were then eluted at room temperature with 250 μl of selection buffer containing either 100 μM (Rounds 1–5) or 50 μM (Rounds 6–10) MDPV. .. Polymerase chain reaction (PCR) was then performed using a BioRad C1000 thermal cycler.

Sample Prep:

Article Title: Targeted Genome Editing in Human Repopulating Hematopoietic Stem Cells
Article Snippet: PCR products were purified using Agencourt AMPure XP beads (Beckman Coulter) and adaptors were added by TruSeq DNA LT Sample Prep Kit (Illumina). .. In order to build an equimolar library, PCR products were quantified with KAPA Library Quantification Kit for Illumina sequencing platforms (KAPABIOSYSTEMS) on C1000 Thermal Cycler (BIO-RAD) and sequenced on MiSeq Illumina Platform using MiSeq Reagent v.3 (Illumina).

Spectrophotometry:

Article Title: Generation and selection of pluripotent stem cells for robust differentiation to insulin-secreting cells capable of reversing diabetes in rodents
Article Snippet: Purity and yield of RNA was confirmed using a NanoDrop 2000c spectrophotometer (ThermoFisher Scientific) and isolated RNA was stored at -80°C until RT-qPCR was performed. .. Reverse transcription to cDNA was performed using the iScript cDNA Synthesis Kit (BioRad #1708890) and a C1000 thermal cycler (BioRad). qPCR was performed using 50 ng cDNA in the SsoAdvanced Universal SYBR Green Supermix (BioRad #1725270), and a CFX96 real-time PCR system paired with a C1000 thermal cycler (BioRad).

Gas Chromatography-Mass Spectrometry:

Article Title: Substrate Control in Stereoselective Lanthionine Biosynthesis
Article Snippet: All polymerase chain reactions (PCR) were carried out on a C1000™ thermal cycler (Bio-Rad). .. LC-ESI-Q/TOF MS and MS/MS analyses were conducted using a Synapt G1 instrument with an Acquity UPLC system (Waters), which was equipped with a Jupiter Proteo C12 column (5 µm; 90 Å; 100 × 1.0 mm) (Phenomenex).

DNA Purification:

Article Title: Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers
Article Snippet: Cross-links were reversed via overnight incubation at 65°C with sodium dodecyl sulfate, and DNA was purified using MinElute DNA purification columns (Qiagen). .. 10ng of DNA was used for subsequent qPCR reactions using a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad).

Recombinant:

Article Title: SUMOylation and the HSF1-Regulated Chaperone Network Converge to Promote Proteostasis in Response to Heat Shock
Article Snippet: Recombinant His6-FoxM1 was purified using TALON metal affinity resin and eluted from beads with 400 mM imidazole (pH 8). .. Recombinant MMS21 or FoxM1 was aliquoted into 11 or 16 samples of 50 μl each and incubated at increasing temperatures from 30–70°C for 5 min using gradient settings on a Bio-Rad C1000™ Thermal Cycler or a Veriti ™ 96-Well Thermal Cycler. .. For the experiments described in C, FoxM1 was aliquoted into samples of 50 μl and incubated for the indicated time periods at the indicated temperatures using a thermomixer (Eppendorf).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Bio-Rad qrt pcr analysis
    Relative mRNA levels of EDNs and EDNRs in E18 chicken retina, primary chicken Müller cells and the human MIO-M1 Müller cell line. <t>qRT-PCR</t> analysis of mRNA levels. Bar graphs showing the relative mRNA levels normalized to ß-actin for (A) EDNRA, EDNRB, EDNRB2 and SOX2, and for (B) EDN1, EDN2, EDN3 in chicken E18 retina (Chicken retina), primary chicken Müller cells (Chicken Müller cell) and the human MIO-M1 Müller cell line. Note that EDNRB2 is not found in human. For the MIO-M1 cells the relative mRNA levels of EDNRA and EDNRB are shown. Sox2 is included as an expression reference for the chicken cells. Bar graphs are mean ± SEM, n = 5 (*P
    Qrt Pcr Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qrt pcr analysis/product/Bio-Rad
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    qrt pcr analysis - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    gfp  (Bio-Rad)
    93
    Bio-Rad gfp
    EBOV uAUGs attenuate translation at primary open reading frames (pORFs). A . Diagram depicting the location of each mutated uAUG codon (to UUG) in the indicated EBOV 5′-UTRs. B . Equal copies of mRNA (determined by real time <t>PCR)</t> encoding <t>GFP</t> downstream of individual 5′-UTRs described in 3A were transfected into 293T cells. At 2.5 hours post transfection, cells were harvested and analyzed by flow cytometry. The fold difference in GFP mean fluorescence intensity (M.F.I.) between the paired samples (with and without the uAUG) is indicated. Each bar represents the mean of triplicate samples. Data for the L 5′-UTR are represented with gray bars. C . A real time PCR measurement of GFP mRNA levels present in the transfected cells described in B was determined for each sample. Each sample was normalized to 18s rRNA. D . The same experiment was performed in parallel as in B, but in primary human dendritic cells. Data are values of single transfections. E and F . Select, representative flow cytometry data from experiments 2B and D; depicted in histogram format showing GFP fluorescence of 293T and dendritic cells which were mock transfected (dashed line), transfected with GFP downstream of the WT L 5′-UTR (solid line) or the mutated L 5′-UTR (dotted line). The data are representative of three independent experiments.
    Gfp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp/product/Bio-Rad
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    gfp - by Bioz Stars, 2020-01
    93/100 stars
      Buy from Supplier

    78
    Bio-Rad sybr green based qrt pcr method
    Expression profiling of BdC genes in different B. distachyon organs detected by <t>qRT-PCR.</t> (A) Comparative expression levels of 10 BdC genes in different Brachypodium distachyon organs, including roots, stems and leaves at the two-leaf stage; seed palea, lemma, flag-leaf at 11, and 23 days after anthesis (DPA). (B) Dynamic expression profiles of 10 BdC genes during seed development in Bd21. Relative quantification of the expression levels in developing caryopses was collected between 6 and 28 DPA. Log transform data was used to create the heatmap. Expression data were obtained from three biological replicates. Differences in gene expression changes are shown in color as the scale.
    Sybr Green Based Qrt Pcr Method, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green based qrt pcr method/product/Bio-Rad
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sybr green based qrt pcr method - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    82
    Bio-Rad mor1 genes
    Relative gene expression of the GluN1 (panel A) and the <t>MOR1</t> (panel B) in the midbrain of control and tolerant groups. Each bar represents mean ± SEM of quantified data related to the expression of the respective genes in each group. * P
    Mor1 Genes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mor1 genes/product/Bio-Rad
    Average 82 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mor1 genes - by Bioz Stars, 2020-01
    82/100 stars
      Buy from Supplier

    Image Search Results


    Relative mRNA levels of EDNs and EDNRs in E18 chicken retina, primary chicken Müller cells and the human MIO-M1 Müller cell line. qRT-PCR analysis of mRNA levels. Bar graphs showing the relative mRNA levels normalized to ß-actin for (A) EDNRA, EDNRB, EDNRB2 and SOX2, and for (B) EDN1, EDN2, EDN3 in chicken E18 retina (Chicken retina), primary chicken Müller cells (Chicken Müller cell) and the human MIO-M1 Müller cell line. Note that EDNRB2 is not found in human. For the MIO-M1 cells the relative mRNA levels of EDNRA and EDNRB are shown. Sox2 is included as an expression reference for the chicken cells. Bar graphs are mean ± SEM, n = 5 (*P

    Journal: PLoS ONE

    Article Title: Endothelin B Receptors on Primary Chicken Müller Cells and the Human MIO-M1 Müller Cell Line Activate ERK Signaling via Transactivation of Epidermal Growth Factor Receptors

    doi: 10.1371/journal.pone.0167778

    Figure Lengend Snippet: Relative mRNA levels of EDNs and EDNRs in E18 chicken retina, primary chicken Müller cells and the human MIO-M1 Müller cell line. qRT-PCR analysis of mRNA levels. Bar graphs showing the relative mRNA levels normalized to ß-actin for (A) EDNRA, EDNRB, EDNRB2 and SOX2, and for (B) EDN1, EDN2, EDN3 in chicken E18 retina (Chicken retina), primary chicken Müller cells (Chicken Müller cell) and the human MIO-M1 Müller cell line. Note that EDNRB2 is not found in human. For the MIO-M1 cells the relative mRNA levels of EDNRA and EDNRB are shown. Sox2 is included as an expression reference for the chicken cells. Bar graphs are mean ± SEM, n = 5 (*P

    Article Snippet: QRT-PCR analysis (IQ SyBr Green Supermix and a C1000 Thermal Cycler; Bio-Rad, Hercules, CA, USA) was performed as previously described [ , ].

    Techniques: Quantitative RT-PCR, Expressing

    Expression of HB-EGF in chicken Müller cells and in human MIO-M1 cell line. QRT-PCR analysis of heparin binding epidermal growth factor (HB-EGF), transcription factor SOX2 and cellular retinaldehyde-binding protein (CRALBP) mRNA levels in primary chicken Müller cell and in human MIO-M1 cell cultures. SOX2 and CRALBP were included as expression references known to be expressed in Müller cells. Bar graph showing the relative mRNA levels in relation to β-actin mRNA levels. Bar graph is mean ±SEM, n > 5.

    Journal: PLoS ONE

    Article Title: Endothelin B Receptors on Primary Chicken Müller Cells and the Human MIO-M1 Müller Cell Line Activate ERK Signaling via Transactivation of Epidermal Growth Factor Receptors

    doi: 10.1371/journal.pone.0167778

    Figure Lengend Snippet: Expression of HB-EGF in chicken Müller cells and in human MIO-M1 cell line. QRT-PCR analysis of heparin binding epidermal growth factor (HB-EGF), transcription factor SOX2 and cellular retinaldehyde-binding protein (CRALBP) mRNA levels in primary chicken Müller cell and in human MIO-M1 cell cultures. SOX2 and CRALBP were included as expression references known to be expressed in Müller cells. Bar graph showing the relative mRNA levels in relation to β-actin mRNA levels. Bar graph is mean ±SEM, n > 5.

    Article Snippet: QRT-PCR analysis (IQ SyBr Green Supermix and a C1000 Thermal Cycler; Bio-Rad, Hercules, CA, USA) was performed as previously described [ , ].

    Techniques: Expressing, Quantitative RT-PCR, Binding Assay

    Endothelins and their receptors in retina after excitotoxic injury. (A) Schematic tree depicting orthologs and paralogs of the endothelin receptors (EDNRs) in Aves and Mammalia. EDNR2B has only been found in non-mammalian species. The tree is based on Ensembl Gene tree ID: ENSGT00760000119177. (B) Interactions between the endothelins (EDNs), the EDNRB agonist IRL1620 and the EDNRs. (C) Experimental outline. QRT-PCR analysis of (D) EDNRA, EDNRB, EDNRB2 and (E) EDN1, EDN2 and EDN3 mRNA levels in NMDA- or vehicle- (Control) treated eyes. Bar graphs show the relative mRNA levels normalized to ß-actin. Bar graphs are mean ± SEM, n = 6 (control 2 h), n = 5 (NMDA 2 h), n = 6 (control 12 h), n = 5 (NMDA 12 h), n = 6 (control 24 h), n = 6 (NMDA 24 h, (*P

    Journal: PLoS ONE

    Article Title: Endothelin B Receptors on Primary Chicken Müller Cells and the Human MIO-M1 Müller Cell Line Activate ERK Signaling via Transactivation of Epidermal Growth Factor Receptors

    doi: 10.1371/journal.pone.0167778

    Figure Lengend Snippet: Endothelins and their receptors in retina after excitotoxic injury. (A) Schematic tree depicting orthologs and paralogs of the endothelin receptors (EDNRs) in Aves and Mammalia. EDNR2B has only been found in non-mammalian species. The tree is based on Ensembl Gene tree ID: ENSGT00760000119177. (B) Interactions between the endothelins (EDNs), the EDNRB agonist IRL1620 and the EDNRs. (C) Experimental outline. QRT-PCR analysis of (D) EDNRA, EDNRB, EDNRB2 and (E) EDN1, EDN2 and EDN3 mRNA levels in NMDA- or vehicle- (Control) treated eyes. Bar graphs show the relative mRNA levels normalized to ß-actin. Bar graphs are mean ± SEM, n = 6 (control 2 h), n = 5 (NMDA 2 h), n = 6 (control 12 h), n = 5 (NMDA 12 h), n = 6 (control 24 h), n = 6 (NMDA 24 h, (*P

    Article Snippet: QRT-PCR analysis (IQ SyBr Green Supermix and a C1000 Thermal Cycler; Bio-Rad, Hercules, CA, USA) was performed as previously described [ , ].

    Techniques: Quantitative RT-PCR

    EBOV uAUGs attenuate translation at primary open reading frames (pORFs). A . Diagram depicting the location of each mutated uAUG codon (to UUG) in the indicated EBOV 5′-UTRs. B . Equal copies of mRNA (determined by real time PCR) encoding GFP downstream of individual 5′-UTRs described in 3A were transfected into 293T cells. At 2.5 hours post transfection, cells were harvested and analyzed by flow cytometry. The fold difference in GFP mean fluorescence intensity (M.F.I.) between the paired samples (with and without the uAUG) is indicated. Each bar represents the mean of triplicate samples. Data for the L 5′-UTR are represented with gray bars. C . A real time PCR measurement of GFP mRNA levels present in the transfected cells described in B was determined for each sample. Each sample was normalized to 18s rRNA. D . The same experiment was performed in parallel as in B, but in primary human dendritic cells. Data are values of single transfections. E and F . Select, representative flow cytometry data from experiments 2B and D; depicted in histogram format showing GFP fluorescence of 293T and dendritic cells which were mock transfected (dashed line), transfected with GFP downstream of the WT L 5′-UTR (solid line) or the mutated L 5′-UTR (dotted line). The data are representative of three independent experiments.

    Journal: PLoS Pathogens

    Article Title: An Upstream Open Reading Frame Modulates Ebola Virus Polymerase Translation and Virus Replication

    doi: 10.1371/journal.ppat.1003147

    Figure Lengend Snippet: EBOV uAUGs attenuate translation at primary open reading frames (pORFs). A . Diagram depicting the location of each mutated uAUG codon (to UUG) in the indicated EBOV 5′-UTRs. B . Equal copies of mRNA (determined by real time PCR) encoding GFP downstream of individual 5′-UTRs described in 3A were transfected into 293T cells. At 2.5 hours post transfection, cells were harvested and analyzed by flow cytometry. The fold difference in GFP mean fluorescence intensity (M.F.I.) between the paired samples (with and without the uAUG) is indicated. Each bar represents the mean of triplicate samples. Data for the L 5′-UTR are represented with gray bars. C . A real time PCR measurement of GFP mRNA levels present in the transfected cells described in B was determined for each sample. Each sample was normalized to 18s rRNA. D . The same experiment was performed in parallel as in B, but in primary human dendritic cells. Data are values of single transfections. E and F . Select, representative flow cytometry data from experiments 2B and D; depicted in histogram format showing GFP fluorescence of 293T and dendritic cells which were mock transfected (dashed line), transfected with GFP downstream of the WT L 5′-UTR (solid line) or the mutated L 5′-UTR (dotted line). The data are representative of three independent experiments.

    Article Snippet: Each cDNA was then subjected to real time quantitative PCR with primers specific for GFP (Bio-Rad C1000 Thermal Cycler).

    Techniques: Real-time Polymerase Chain Reaction, Transfection, Flow Cytometry, Cytometry, Fluorescence

    Efficiency of translation initiation at L uAUG is determined by its sequence context. A . L 5′-UTR GFP mRNA reporter constructs were generated such that the uORF is fused in frame to GFP. The nucleotide sequence immediately surrounding the uAUG of each construct is displayed. The first construct (uORF-GFP) includes the first 46 nucleotides of the L 5′-UTR up to the uAUG followed by the entire L uORF sequence placed in frame with the GFP ORF (labeled uORF-GFP). The middle construct (uORF-GFP SK) is identical to the first, but the uAUG is surrounded by a strong Kozak sequence (A at the −3 position and a G at the +4 position, where the A of the AUG is designated as +1). The bottom construct includes the L 5′-UTR, through the uAUG and the second codon of the uORF which was placed in frame with the GFP ORF, but lacks the rest of the uORF. In each case, the start codon for GFP was removed. The number of nucleotides in each construct is indicated and the features of each construct are summarized in the box above the diagram. B . Equal amounts of each in vitro transcribed mRNA were transfected into 293T cells. At 2.5 hours post transfection, cells were harvested and analyzed by flow cytometry. The experiment was performed in triplicate, and a representative sample is displayed for each group. C . GFP mRNA levels, as determined by real time PCR, present in the transfected cells described in B were determined for each sample.

    Journal: PLoS Pathogens

    Article Title: An Upstream Open Reading Frame Modulates Ebola Virus Polymerase Translation and Virus Replication

    doi: 10.1371/journal.ppat.1003147

    Figure Lengend Snippet: Efficiency of translation initiation at L uAUG is determined by its sequence context. A . L 5′-UTR GFP mRNA reporter constructs were generated such that the uORF is fused in frame to GFP. The nucleotide sequence immediately surrounding the uAUG of each construct is displayed. The first construct (uORF-GFP) includes the first 46 nucleotides of the L 5′-UTR up to the uAUG followed by the entire L uORF sequence placed in frame with the GFP ORF (labeled uORF-GFP). The middle construct (uORF-GFP SK) is identical to the first, but the uAUG is surrounded by a strong Kozak sequence (A at the −3 position and a G at the +4 position, where the A of the AUG is designated as +1). The bottom construct includes the L 5′-UTR, through the uAUG and the second codon of the uORF which was placed in frame with the GFP ORF, but lacks the rest of the uORF. In each case, the start codon for GFP was removed. The number of nucleotides in each construct is indicated and the features of each construct are summarized in the box above the diagram. B . Equal amounts of each in vitro transcribed mRNA were transfected into 293T cells. At 2.5 hours post transfection, cells were harvested and analyzed by flow cytometry. The experiment was performed in triplicate, and a representative sample is displayed for each group. C . GFP mRNA levels, as determined by real time PCR, present in the transfected cells described in B were determined for each sample.

    Article Snippet: Each cDNA was then subjected to real time quantitative PCR with primers specific for GFP (Bio-Rad C1000 Thermal Cycler).

    Techniques: Sequencing, Construct, Generated, Labeling, In Vitro, Transfection, Flow Cytometry, Cytometry, Real-time Polymerase Chain Reaction

    Suppression of pORF translation by the uAUG in the L 5′-UTR is position dependent. A . Diagram depicting wild-type and mutated L 5′-UTR-GFP reporter constructs in which the location of the uAUG was altered. For the L uORF, the black box represents the L uORF sequence, while the gray box depicts the remainder of the overlapping uORF that consists of GFP-derived sequences. Nucleotide changes that differ from the wildtype L 5′-UTR sequence are indicated in bold and the underlined sequences highlight the uORFs. Additional mutations were introduced to preserve the Kozak sequence at the −3 and +4 position such that they match that present in the wildtype L 5′-UTR. The reading frame of the uORF for each construct is indicated on the right. B . Equal amounts of in vitro transcribed mRNAs corresponding to the constructs depicted in panel A were transfected into 293T cells. At 2.5 hours post transfection, cells were harvested and analyzed by flow cytometry for GFP fluorescence. Each bar represents the mean of triplicate samples, and all values were calculated relative to the B-actin 5′-UTR GFP control mRNA which was set to 100%. C . A real time PCR measurement of GFP mRNA levels present in the transfected cells described in B was determined for each sample.

    Journal: PLoS Pathogens

    Article Title: An Upstream Open Reading Frame Modulates Ebola Virus Polymerase Translation and Virus Replication

    doi: 10.1371/journal.ppat.1003147

    Figure Lengend Snippet: Suppression of pORF translation by the uAUG in the L 5′-UTR is position dependent. A . Diagram depicting wild-type and mutated L 5′-UTR-GFP reporter constructs in which the location of the uAUG was altered. For the L uORF, the black box represents the L uORF sequence, while the gray box depicts the remainder of the overlapping uORF that consists of GFP-derived sequences. Nucleotide changes that differ from the wildtype L 5′-UTR sequence are indicated in bold and the underlined sequences highlight the uORFs. Additional mutations were introduced to preserve the Kozak sequence at the −3 and +4 position such that they match that present in the wildtype L 5′-UTR. The reading frame of the uORF for each construct is indicated on the right. B . Equal amounts of in vitro transcribed mRNAs corresponding to the constructs depicted in panel A were transfected into 293T cells. At 2.5 hours post transfection, cells were harvested and analyzed by flow cytometry for GFP fluorescence. Each bar represents the mean of triplicate samples, and all values were calculated relative to the B-actin 5′-UTR GFP control mRNA which was set to 100%. C . A real time PCR measurement of GFP mRNA levels present in the transfected cells described in B was determined for each sample.

    Article Snippet: Each cDNA was then subjected to real time quantitative PCR with primers specific for GFP (Bio-Rad C1000 Thermal Cycler).

    Techniques: Construct, Sequencing, Derivative Assay, In Vitro, Transfection, Flow Cytometry, Cytometry, Fluorescence, Real-time Polymerase Chain Reaction

    A screen of EBOV 5' UTRs demonstrates that the polymerase (L) 5'-UTR suppresses GFP expression. A . Diagram of the in vitro generated mRNAs used to assess the impact of 5′-UTRs on translation efficiency. EBOV 5′-UTRs or the β-actin control 5′-UTR were placed upstream of a GFP reporter ORF. Distances in nucleotides from the pAUG are indicated at the top. uAUGs are indicated by (S) and stop codons for uORFs are indicated by (X). Within the UTRs, black boxes indicate uORFs out of frame with GFP, while white boxes indicate uORFs in frame with GFP. The lengths (in nucleotides, including stop codons) of the uORFs are indicated. For the L uORF, the black box represents the L uORF sequence, while the gray box depicts the remainder of the overlapping uORF that consists of GFP-derived sequences. The UTRs are listed according to their order in the EBOV genome. B . Mean GFP fluorescence intensity (M.F.I.) of 293T cells transfected with equal copies (determined by real time PCR) of in vitro transcribed mRNAs encoding GFP corresponding to those diagramed in A. At 2.5 hours post transfection, cells were harvested and analyzed by flow cytometry for GFP expression. The bars are presented according to the order of genes in the EBOV genome, and data highlighting the L 5′-UTR is indicated by a gray bar. Data are the mean of triplicate samples. C . A real time PCR measurement of GFP mRNA levels present in the transfected cells described in B was determined for each sample. Each sample was normalized to 18s rRNA. D . The same mRNAs transfected in B were transfected into primary human dendritic cells (DC). Data are the values from single transfections. E and F . Selected GFP expression data from the experiments described in 2B and D, but depicted in histogram format. 293T cells and dendritic cells were mock transfected (dashed line), or transfected with GFP downstream of either the β-actin (dotted line) or L 5′-UTR (solid line), indicating the L 5′-UTR suppresses GFP expression. The data are representative of three independent experiments.

    Journal: PLoS Pathogens

    Article Title: An Upstream Open Reading Frame Modulates Ebola Virus Polymerase Translation and Virus Replication

    doi: 10.1371/journal.ppat.1003147

    Figure Lengend Snippet: A screen of EBOV 5' UTRs demonstrates that the polymerase (L) 5'-UTR suppresses GFP expression. A . Diagram of the in vitro generated mRNAs used to assess the impact of 5′-UTRs on translation efficiency. EBOV 5′-UTRs or the β-actin control 5′-UTR were placed upstream of a GFP reporter ORF. Distances in nucleotides from the pAUG are indicated at the top. uAUGs are indicated by (S) and stop codons for uORFs are indicated by (X). Within the UTRs, black boxes indicate uORFs out of frame with GFP, while white boxes indicate uORFs in frame with GFP. The lengths (in nucleotides, including stop codons) of the uORFs are indicated. For the L uORF, the black box represents the L uORF sequence, while the gray box depicts the remainder of the overlapping uORF that consists of GFP-derived sequences. The UTRs are listed according to their order in the EBOV genome. B . Mean GFP fluorescence intensity (M.F.I.) of 293T cells transfected with equal copies (determined by real time PCR) of in vitro transcribed mRNAs encoding GFP corresponding to those diagramed in A. At 2.5 hours post transfection, cells were harvested and analyzed by flow cytometry for GFP expression. The bars are presented according to the order of genes in the EBOV genome, and data highlighting the L 5′-UTR is indicated by a gray bar. Data are the mean of triplicate samples. C . A real time PCR measurement of GFP mRNA levels present in the transfected cells described in B was determined for each sample. Each sample was normalized to 18s rRNA. D . The same mRNAs transfected in B were transfected into primary human dendritic cells (DC). Data are the values from single transfections. E and F . Selected GFP expression data from the experiments described in 2B and D, but depicted in histogram format. 293T cells and dendritic cells were mock transfected (dashed line), or transfected with GFP downstream of either the β-actin (dotted line) or L 5′-UTR (solid line), indicating the L 5′-UTR suppresses GFP expression. The data are representative of three independent experiments.

    Article Snippet: Each cDNA was then subjected to real time quantitative PCR with primers specific for GFP (Bio-Rad C1000 Thermal Cycler).

    Techniques: Expressing, In Vitro, Generated, Sequencing, Derivative Assay, Fluorescence, Transfection, Real-time Polymerase Chain Reaction, Flow Cytometry, Cytometry

    pORF translation is suppressed by a strong uAUG Kozak sequence in the L 5′-UTR, but is not affected by a weak uAUG Kozak sequence. A . Diagram depicting the in vitro generated L 5′-UTR GFP mRNA reporter constructs with permutations surrounding the L uAUG. For the L uORF, the black box represents the L uORF sequence, while the gray box depicts the remainder of the overlapping uORF that consists of GFP-derived sequences. Each sequence surrounding the uAUG above the diagram represents a reporter construct. The top is the wildtype nucleotide sequence surrounding the L uAUG (WT L 5′-UTR). The next construct has a strong Kozak sequence (A at the −3 position and a G at the +4 position, where the A of the AUG is designated as +1). Two constructs predicted to have a weak Kozak sequence (uAUG WK1 and WK2) have mutations at the +4 and +4/+5 positions, respectively. The construct labeled “uAUG STOP” has a point mutation that incorporates a stop codon directly after the uAUG start codon. Finally, uUUG and uUCG both ablate the uAUG start codon. Each construct is analyzed in panel B. B . Equal amounts of each in vitro transcribed mRNA were transfected into 293T cells. At 2.5 hours post transfection, cells were harvested and analyzed by flow cytometry. Each bar represents the mean and standard deviation of triplicate samples, and all values were calculated relative to the B-actin 5′-UTR GFP control mRNA, which was set to 100%. C . A real time PCR measurement of GFP mRNA levels present in the transfected cells described in B was determined for each sample.

    Journal: PLoS Pathogens

    Article Title: An Upstream Open Reading Frame Modulates Ebola Virus Polymerase Translation and Virus Replication

    doi: 10.1371/journal.ppat.1003147

    Figure Lengend Snippet: pORF translation is suppressed by a strong uAUG Kozak sequence in the L 5′-UTR, but is not affected by a weak uAUG Kozak sequence. A . Diagram depicting the in vitro generated L 5′-UTR GFP mRNA reporter constructs with permutations surrounding the L uAUG. For the L uORF, the black box represents the L uORF sequence, while the gray box depicts the remainder of the overlapping uORF that consists of GFP-derived sequences. Each sequence surrounding the uAUG above the diagram represents a reporter construct. The top is the wildtype nucleotide sequence surrounding the L uAUG (WT L 5′-UTR). The next construct has a strong Kozak sequence (A at the −3 position and a G at the +4 position, where the A of the AUG is designated as +1). Two constructs predicted to have a weak Kozak sequence (uAUG WK1 and WK2) have mutations at the +4 and +4/+5 positions, respectively. The construct labeled “uAUG STOP” has a point mutation that incorporates a stop codon directly after the uAUG start codon. Finally, uUUG and uUCG both ablate the uAUG start codon. Each construct is analyzed in panel B. B . Equal amounts of each in vitro transcribed mRNA were transfected into 293T cells. At 2.5 hours post transfection, cells were harvested and analyzed by flow cytometry. Each bar represents the mean and standard deviation of triplicate samples, and all values were calculated relative to the B-actin 5′-UTR GFP control mRNA, which was set to 100%. C . A real time PCR measurement of GFP mRNA levels present in the transfected cells described in B was determined for each sample.

    Article Snippet: Each cDNA was then subjected to real time quantitative PCR with primers specific for GFP (Bio-Rad C1000 Thermal Cycler).

    Techniques: Sequencing, In Vitro, Generated, Construct, Derivative Assay, Labeling, Mutagenesis, Transfection, Flow Cytometry, Cytometry, Standard Deviation, Real-time Polymerase Chain Reaction

    Expression profiling of BdC genes in different B. distachyon organs detected by qRT-PCR. (A) Comparative expression levels of 10 BdC genes in different Brachypodium distachyon organs, including roots, stems and leaves at the two-leaf stage; seed palea, lemma, flag-leaf at 11, and 23 days after anthesis (DPA). (B) Dynamic expression profiles of 10 BdC genes during seed development in Bd21. Relative quantification of the expression levels in developing caryopses was collected between 6 and 28 DPA. Log transform data was used to create the heatmap. Expression data were obtained from three biological replicates. Differences in gene expression changes are shown in color as the scale.

    Journal: Frontiers in Plant Science

    Article Title: Molecular Characterization and Expression Profiling of Brachypodium distachyon L. Cystatin Genes Reveal High Evolutionary Conservation and Functional Divergence in Response to Abiotic Stress

    doi: 10.3389/fpls.2017.00743

    Figure Lengend Snippet: Expression profiling of BdC genes in different B. distachyon organs detected by qRT-PCR. (A) Comparative expression levels of 10 BdC genes in different Brachypodium distachyon organs, including roots, stems and leaves at the two-leaf stage; seed palea, lemma, flag-leaf at 11, and 23 days after anthesis (DPA). (B) Dynamic expression profiles of 10 BdC genes during seed development in Bd21. Relative quantification of the expression levels in developing caryopses was collected between 6 and 28 DPA. Log transform data was used to create the heatmap. Expression data were obtained from three biological replicates. Differences in gene expression changes are shown in color as the scale.

    Article Snippet: Transcript levels of cystatins genes were analyzed at relative levels by using the SYBR Green-based qRT-PCR method (CFX96, Bio-Rad Thermal Cycler system C1000 series).

    Techniques: Expressing, Quantitative RT-PCR

    Expression profiles of BdC family members in the leaves of B. distachyon in response to different abiotic stress treatments (cold, H 2 O 2 , CdCl 2 , drought, salt, and ABA) by qRT-PCR . Blocks with colors indicated decreased (green) or increased (red) transcript accumulation relative to the respective control. Filled squares indicate a significant difference from the control ( p

    Journal: Frontiers in Plant Science

    Article Title: Molecular Characterization and Expression Profiling of Brachypodium distachyon L. Cystatin Genes Reveal High Evolutionary Conservation and Functional Divergence in Response to Abiotic Stress

    doi: 10.3389/fpls.2017.00743

    Figure Lengend Snippet: Expression profiles of BdC family members in the leaves of B. distachyon in response to different abiotic stress treatments (cold, H 2 O 2 , CdCl 2 , drought, salt, and ABA) by qRT-PCR . Blocks with colors indicated decreased (green) or increased (red) transcript accumulation relative to the respective control. Filled squares indicate a significant difference from the control ( p

    Article Snippet: Transcript levels of cystatins genes were analyzed at relative levels by using the SYBR Green-based qRT-PCR method (CFX96, Bio-Rad Thermal Cycler system C1000 series).

    Techniques: Expressing, Quantitative RT-PCR

    Relative gene expression of the GluN1 (panel A) and the MOR1 (panel B) in the midbrain of control and tolerant groups. Each bar represents mean ± SEM of quantified data related to the expression of the respective genes in each group. * P

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Association of morphine-induced analgesic tolerance with changes in gene expression of GluN1 and MOR1 in rat spinal cord and midbrain

    doi:

    Figure Lengend Snippet: Relative gene expression of the GluN1 (panel A) and the MOR1 (panel B) in the midbrain of control and tolerant groups. Each bar represents mean ± SEM of quantified data related to the expression of the respective genes in each group. * P

    Article Snippet: Separate multiplex PCR were performed to amplify related cDNAs of the β-actin (as control) and the GluN1 or MOR1 genes (C1000 Thermal Cycler, BIO-RAD, USA).

    Techniques: Expressing

    Relative gene expression of the GluN1 (panel A) and the MOR1 (panel B) in the lumbosacral cord in control and tolerant groups. Each bar represents mean ± SEM of quantified data related to the expression of the respective genes in each group. ** P

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Association of morphine-induced analgesic tolerance with changes in gene expression of GluN1 and MOR1 in rat spinal cord and midbrain

    doi:

    Figure Lengend Snippet: Relative gene expression of the GluN1 (panel A) and the MOR1 (panel B) in the lumbosacral cord in control and tolerant groups. Each bar represents mean ± SEM of quantified data related to the expression of the respective genes in each group. ** P

    Article Snippet: Separate multiplex PCR were performed to amplify related cDNAs of the β-actin (as control) and the GluN1 or MOR1 genes (C1000 Thermal Cycler, BIO-RAD, USA).

    Techniques: Expressing