bodipy fl phosphatidylinositol (Echelon Biosciences)


Structured Review

Bodipy Fl Phosphatidylinositol, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bodipy fl phosphatidylinositol/product/Echelon Biosciences
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Local PI(4,5)P 2 generation controls fusion pore expansion during exocytosis"
Article Title: Local PI(4,5)P 2 generation controls fusion pore expansion during exocytosis
Journal: bioRxiv
doi: 10.1101/2022.01.26.477824

Figure Legend Snippet: (A) Representative example of an INS1 cell expressing the PI(4,5)P 2 sensor PH-PLCδ1 in unstimulated conditions (max pixel intensities for 10 s before stimulation, left), and during stimulation with elevated K + (max pixel intensities during first 20 s of stimulation, right). (B) Three examples (1-3) of single granule exocytosis in K + stimulated cells, as in A. Image frames (upper) show granule (gr) and PI(4,5)P 2 sensor (PIP 2 ) images at the exocytosis site (1s/frame, scalebar 1μm), and traces the quantification of gr (middle) and PIP 2 signal (lower) temporally aligned to the same events. Note temporary increase of gr fluorescence (indicating FR events with arrested fusion pore) and associated PI(4,5)P 2 -signal in examples 1-2, but not 3 (IR events). Black arrows indicate moment of fusion pore opening and content release, red the fusion pore lifetime. (C) Cartoons illustrating the interpretation of events in B. (D-F) Dynamics of phosphatidylinositol species at FR (black) and IR (grey) exocytosis events, quantified as average ΔF/S (see ) for (D) the PI(4)P sensor EGFP-P4M-SidM (107 FR events and 84 IR events in 26 INS1-cells, 3 preps), (E) the PI(4,5)P 2 sensor EGFP-PH-PLCδ1 (164 FR events, 83 IR events in 24 INS1-cells, 3 preps), and (F) the PI(3,4,5)P 3 sensor 4EGFP-GRP1 (83 events in 15 INS1-cells, 3 preps). All signals were aligned to the moment of release (t=0); positive ΔF/S indicates excess sensor fluorescence at the exocytosis site. (G-H) As in D-F but for human islets cells, and aligned to the moment of fusion. (G) PI(4,5)P 2 (228 FR events, 102 IR events in 33 cells), (H) PI(4)P (191 FR events, 234 IR events in 30 cells, 3 preps). (I) PI(4,5)P 2 accumulation (ΔΔF/S) as function of fusion pore lifetime in INS1-cells (I) and human islet cells (J); same experiments as in D and H; circle size indicates events per bin.
Techniques Used: Expressing, Fluorescence

Figure Legend Snippet: (A) Cartoon illustrating phosphatidylinositol synthesis steps. (B) Quantification of PIP5k1c expression in cells expressing scrambled control (scr) or PIP5k1c specific shRNA (K5KD). (C) Exocytosis during 40 s of K + -stimulation quantified in cells expressing NPY. The cells coexpressed combinations of scr, K5KD, EGFP-PH-PLCδ1, and amphiphysin-mCherry, as indicated (scr+PLCδ1, 18 cells; K5KD+PLCδ1, 38 cells; scr+amphiphysin, 16 cells; K5KD+amphiphysin, 38 cells. Values are p-values for scr vs K5KD, non-paired t-test. (D) Fraction of FR events in experiments in C, Values indicate p-values, u-test. (E) Cumulative frequency histograms of NPY release times in C, for scr+PH-PLCδ1 (grey) and K5KD+PH-PLCδ1 (light blue). P=0.006, KS test (F) As in (E) but for scr or K5KD + amphiphysin. P=1.8E-5, KS test. (G) Examples of single granule exocytosis (top), average time course of PH-PLCδ1 signal (ΔF/S ±SEM; lower left), and distribution of the peak amplitudes (ΔΔF/S, non-paired t-test; lower right) for scr+PH-PLCδ1 (grey; 210 FR events in 18 cells, 3 preps) and K5KD+PH-PLCδ1 (light blue; 40 FR events in 38 cells). Same experiments as in C-E. (H) As in (G) but for scr+amphiphysin (grey; 249 FR events, 16 cells) and K5KD+amphiphysin (brown; 32 events, 38 cells).
Techniques Used: Expressing, shRNA

Figure Legend Snippet: (A) Cartoon illustrating phosphatidylinositol synthesis. (B) Quantification of mirRNA based knockout of PI4K2a (K4KD) compared with control INS1 cells. (C-E) Average EGFP-PH-PLCδ1 time course (ΔF/S ±SEM) during K + -stimulation in cells co-expressing NPY-mOrange2; for ctrl (E, 225 events), K4KD (D, 291 events), or ctrl with kinase-4 inhibitor A1 (E, 110 events, 100nM A1 for 10 minutes). All events are spatiotemporally aligned to exocytosis events in the NPY-mOrange2 signal. (F) Distribution of peak amplitudes (ΔΔF/S ±SEM) for transients in C-E, p-values shown are for one-way ANOVA, Fisher posthoc test. (G) Exocytosis during 40 s K + -stimulation in C-E, for control (27 cells), K4KD (33 cells), and A1 (13 cells). p-values are for one-way ANOVA, Fisher posthoc test. (H-L) As in C-G, but for cells expressing EGFP-P4M-SidM in control (H, 264 events, 32 cells), K4KD (I, 245 events, 31 cells), or A1 (J, 103 events, 9 cells). (M-N) Average amphiphysin-mCherry time course for FR exocytosis events (ΔF/S ±SEM, aligned to rising phase of co-expressed NPYmOrange2 signal) in ctrl (M, 291 FR events) and with A1 (N, 142 FR events). (O) Fraction of FR events in cells expressing NPY-EGFP alone (C), or together with amphiphysin-mCherry (Amph), and in presence or absence of A1; ctrl (23 cells), control with A1 (13 cells), Amph (14 cells) or Amph with A1 (13 cells); p-values shown are for u-test. (P) Distribution of peak amplitudes (ΔΔF/S) for events in M-N (p-values for non-paired t-test). (R) Cumulative frequency histograms of NPY release times in (O). P=0.39 for ctrl vs A1, P=0.12 for Amph+A1 vs Amph, and P=1.5E-11 for Amph vs ctrl, KS-test).
Techniques Used: Knock-Out, Expressing
bodipy fl phosphatidylinositol (Echelon Biosciences)


Structured Review

Bodipy Fl Phosphatidylinositol, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bodipy fl phosphatidylinositol/product/Echelon Biosciences
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Local PI(4,5)P 2 generation controls fusion pore expansion during exocytosis"
Article Title: Local PI(4,5)P 2 generation controls fusion pore expansion during exocytosis
Journal: bioRxiv
doi: 10.1101/2022.01.26.477824

Figure Legend Snippet: (A) Representative example of an INS1 cell expressing the PI(4,5)P 2 sensor PH-PLCδ1 in unstimulated conditions (max pixel intensities for 10 s before stimulation, left), and during stimulation with elevated K + (max pixel intensities during first 20 s of stimulation, right). (B) Three examples (1-3) of single granule exocytosis in K + stimulated cells, as in A. Image frames (upper) show granule (gr) and PI(4,5)P 2 sensor (PIP 2 ) images at the exocytosis site (1s/frame, scalebar 1μm), and traces the quantification of gr (middle) and PIP 2 signal (lower) temporally aligned to the same events. Note temporary increase of gr fluorescence (indicating FR events with arrested fusion pore) and associated PI(4,5)P 2 -signal in examples 1-2, but not 3 (IR events). Black arrows indicate moment of fusion pore opening and content release, red the fusion pore lifetime. (C) Cartoons illustrating the interpretation of events in B. (D-F) Dynamics of phosphatidylinositol species at FR (black) and IR (grey) exocytosis events, quantified as average ΔF/S (see ) for (D) the PI(4)P sensor EGFP-P4M-SidM (107 FR events and 84 IR events in 26 INS1-cells, 3 preps), (E) the PI(4,5)P 2 sensor EGFP-PH-PLCδ1 (164 FR events, 83 IR events in 24 INS1-cells, 3 preps), and (F) the PI(3,4,5)P 3 sensor 4EGFP-GRP1 (83 events in 15 INS1-cells, 3 preps). All signals were aligned to the moment of release (t=0); positive ΔF/S indicates excess sensor fluorescence at the exocytosis site. (G-H) As in D-F but for human islets cells, and aligned to the moment of fusion. (G) PI(4,5)P 2 (228 FR events, 102 IR events in 33 cells), (H) PI(4)P (191 FR events, 234 IR events in 30 cells, 3 preps). (I) PI(4,5)P 2 accumulation (ΔΔF/S) as function of fusion pore lifetime in INS1-cells (I) and human islet cells (J); same experiments as in D and H; circle size indicates events per bin.
Techniques Used: Expressing, Fluorescence

Figure Legend Snippet: (A) Cartoon illustrating phosphatidylinositol synthesis steps. (B) Quantification of PIP5k1c expression in cells expressing scrambled control (scr) or PIP5k1c specific shRNA (K5KD). (C) Exocytosis during 40 s of K + -stimulation quantified in cells expressing NPY. The cells coexpressed combinations of scr, K5KD, EGFP-PH-PLCδ1, and amphiphysin-mCherry, as indicated (scr+PLCδ1, 18 cells; K5KD+PLCδ1, 38 cells; scr+amphiphysin, 16 cells; K5KD+amphiphysin, 38 cells. Values are p-values for scr vs K5KD, non-paired t-test. (D) Fraction of FR events in experiments in C, Values indicate p-values, u-test. (E) Cumulative frequency histograms of NPY release times in C, for scr+PH-PLCδ1 (grey) and K5KD+PH-PLCδ1 (light blue). P=0.006, KS test (F) As in (E) but for scr or K5KD + amphiphysin. P=1.8E-5, KS test. (G) Examples of single granule exocytosis (top), average time course of PH-PLCδ1 signal (ΔF/S ±SEM; lower left), and distribution of the peak amplitudes (ΔΔF/S, non-paired t-test; lower right) for scr+PH-PLCδ1 (grey; 210 FR events in 18 cells, 3 preps) and K5KD+PH-PLCδ1 (light blue; 40 FR events in 38 cells). Same experiments as in C-E. (H) As in (G) but for scr+amphiphysin (grey; 249 FR events, 16 cells) and K5KD+amphiphysin (brown; 32 events, 38 cells).
Techniques Used: Expressing, shRNA

Figure Legend Snippet: (A) Cartoon illustrating phosphatidylinositol synthesis. (B) Quantification of mirRNA based knockout of PI4K2a (K4KD) compared with control INS1 cells. (C-E) Average EGFP-PH-PLCδ1 time course (ΔF/S ±SEM) during K + -stimulation in cells co-expressing NPY-mOrange2; for ctrl (E, 225 events), K4KD (D, 291 events), or ctrl with kinase-4 inhibitor A1 (E, 110 events, 100nM A1 for 10 minutes). All events are spatiotemporally aligned to exocytosis events in the NPY-mOrange2 signal. (F) Distribution of peak amplitudes (ΔΔF/S ±SEM) for transients in C-E, p-values shown are for one-way ANOVA, Fisher posthoc test. (G) Exocytosis during 40 s K + -stimulation in C-E, for control (27 cells), K4KD (33 cells), and A1 (13 cells). p-values are for one-way ANOVA, Fisher posthoc test. (H-L) As in C-G, but for cells expressing EGFP-P4M-SidM in control (H, 264 events, 32 cells), K4KD (I, 245 events, 31 cells), or A1 (J, 103 events, 9 cells). (M-N) Average amphiphysin-mCherry time course for FR exocytosis events (ΔF/S ±SEM, aligned to rising phase of co-expressed NPYmOrange2 signal) in ctrl (M, 291 FR events) and with A1 (N, 142 FR events). (O) Fraction of FR events in cells expressing NPY-EGFP alone (C), or together with amphiphysin-mCherry (Amph), and in presence or absence of A1; ctrl (23 cells), control with A1 (13 cells), Amph (14 cells) or Amph with A1 (13 cells); p-values shown are for u-test. (P) Distribution of peak amplitudes (ΔΔF/S) for events in M-N (p-values for non-paired t-test). (R) Cumulative frequency histograms of NPY release times in (O). P=0.39 for ctrl vs A1, P=0.12 for Amph+A1 vs Amph, and P=1.5E-11 for Amph vs ctrl, KS-test).
Techniques Used: Knock-Out, Expressing
bodipy fl labelled phosphatidylinositol lipids (Echelon Biosciences)


Structured Review

Bodipy Fl Labelled Phosphatidylinositol Lipids, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bodipy fl labelled phosphatidylinositol lipids/product/Echelon Biosciences
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Insight into the PTEN – p85α interaction and lipid binding properties of the p85α BH domain"
Article Title: Insight into the PTEN – p85α interaction and lipid binding properties of the p85α BH domain
Journal: Oncotarget
doi: 10.18632/oncotarget.26432

Figure Legend Snippet: ( A ) PIP strip nitrocellulose membrane with spotted lipids (100 pmol per spot). ( B ) PIP strips were blocked and probed with the indicated purified p85α protein (37 nM). Bound proteins were detected with an anti-p85α BH antibody, followed with an infrared secondary antibody and visualized using a LICOR Odyssey infrared scanner. PI = phosphatidylinositol; PI3P = phosphatidylinositol 3-phosphate; PI4P = phosphatidylinositol 4-phosphate; PI5P = phosphatidylinositol 5-phosphate; PI3,4P 2 = phosphatidylinositol 3,4-bisphosphate; PI3,5P 2 = phosphatidylinositol 3,5-bisphosphate; PI4,5P 2 = phosphatidylinositol 4,5-bisphosphate; PI3,4,5P 3 = phosphatidylinositol 3,4,5-trisphosphate.
Techniques Used: Stripping Membranes, Purification

Figure Legend Snippet: ( A ) Changes in fluorescence polarization were measured for Bodipy-FL labelled phosphatidylinositol lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.
Techniques Used: Fluorescence, Labeling, Mutagenesis
pibodipy (Echelon Biosciences)


Structured Review
Pibodipy, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pibodipy/product/Echelon Biosciences
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
bodipy fl phosphatidylinositol 3 phosphate (Echelon Biosciences)


Structured Review
Bodipy Fl Phosphatidylinositol 3 Phosphate, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bodipy fl phosphatidylinositol 3 phosphate/product/Echelon Biosciences
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
bodipy fl phosphatidylinositol (Echelon Biosciences)


Structured Review

Bodipy Fl Phosphatidylinositol, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bodipy fl phosphatidylinositol/product/Echelon Biosciences
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "The RNA-Binding Site of Poliovirus 3C Protein Doubles as a Phosphoinositide-Binding Domain"
Article Title: The RNA-Binding Site of Poliovirus 3C Protein Doubles as a Phosphoinositide-Binding Domain
Journal: Structure (London, England : 1993)
doi: 10.1016/j.str.2017.11.001

Figure Legend Snippet: Docking Analysis Reveals a Putative Phosphatidylinositol 4-Phosphate-Binding Site Near the N Terminus of Poliovirus 3C (A) Two clusters were identified by docking dibutyl-PI4P (red spheres) on 3C surface (gray ribbon). The major cluster (94% of the trials) encompasses residues K12, R13, and R84; the minor cluster (6% of the trials) encompasses residues K108 and R143. One hundred docking runs were performed. The crystal structure of 3C (PDB: 1L1N ) was obtained from the PDB (
Techniques Used: Binding Assay, Sequencing

Figure Legend Snippet:
Techniques Used: Recombinant, Protease Inhibitor, Clone Assay, Mutagenesis, Plasmid Preparation, Software
c 00f6 (Echelon Biosciences)


Structured Review

C 00f6, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c 00f6/product/Echelon Biosciences
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "The RNA-Binding Site of Poliovirus 3C Protein Doubles as a Phosphoinositide-Binding Domain"
Article Title: The RNA-Binding Site of Poliovirus 3C Protein Doubles as a Phosphoinositide-Binding Domain
Journal: Structure (London, England : 1993)
doi: 10.1016/j.str.2017.11.001

Figure Legend Snippet:
Techniques Used: Recombinant, Protease Inhibitor, Clone Assay, Mutagenesis, Plasmid Preparation, Software
bodipy fl phosphatidylinositol (Echelon Biosciences)


Structured Review
Bodipy Fl Phosphatidylinositol, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bodipy fl phosphatidylinositol/product/Echelon Biosciences
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
bodipy fl ptdins 4 p (Echelon Biosciences)


Structured Review
Bodipy Fl Ptdins 4 P, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bodipy fl ptdins 4 p/product/Echelon Biosciences
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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bodipy fl ptdins 4 p echelon biosciences (Echelon Biosciences)


Structured Review
Bodipy Fl Ptdins 4 P Echelon Biosciences, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bodipy fl ptdins 4 p echelon biosciences/product/Echelon Biosciences
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
phosphoinositides (Echelon Biosciences)


Structured Review
Phosphoinositides, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphoinositides/product/Echelon Biosciences
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99