bodipy fl labelled phosphatidylinositol lipids  (Echelon Biosciences)


Bioz Verified Symbol Echelon Biosciences is a verified supplier
Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Echelon Biosciences bodipy fl labelled phosphatidylinositol lipids
    Rotational anisotropy analysis of the lipid binding affinities for the wild type p85α BH domain and several p85α BH domain mutants ( A ) Changes in fluorescence polarization were measured for <t>Bodipy-FL</t> labelled <t>phosphatidylinositol</t> lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.
    Bodipy Fl Labelled Phosphatidylinositol Lipids, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bodipy fl labelled phosphatidylinositol lipids/product/Echelon Biosciences
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bodipy fl labelled phosphatidylinositol lipids - by Bioz Stars, 2022-08
    90/100 stars

    Images

    1) Product Images from "Insight into the PTEN – p85α interaction and lipid binding properties of the p85α BH domain"

    Article Title: Insight into the PTEN – p85α interaction and lipid binding properties of the p85α BH domain

    Journal: Oncotarget

    doi: 10.18632/oncotarget.26432

    Rotational anisotropy analysis of the lipid binding affinities for the wild type p85α BH domain and several p85α BH domain mutants ( A ) Changes in fluorescence polarization were measured for Bodipy-FL labelled phosphatidylinositol lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.
    Figure Legend Snippet: Rotational anisotropy analysis of the lipid binding affinities for the wild type p85α BH domain and several p85α BH domain mutants ( A ) Changes in fluorescence polarization were measured for Bodipy-FL labelled phosphatidylinositol lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.

    Techniques Used: Binding Assay, Fluorescence, Labeling, Mutagenesis

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • bodipy fl labelled phosphatidylinositol lipids  (Echelon Biosciences)
    90
    Echelon Biosciences bodipy fl labelled phosphatidylinositol lipids
    Rotational anisotropy analysis of the lipid binding affinities for the wild type p85α BH domain and several p85α BH domain mutants ( A ) Changes in fluorescence polarization were measured for <t>Bodipy-FL</t> labelled <t>phosphatidylinositol</t> lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.
    Bodipy Fl Labelled Phosphatidylinositol Lipids, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bodipy fl labelled phosphatidylinositol lipids/product/Echelon Biosciences
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bodipy fl labelled phosphatidylinositol lipids - by Bioz Stars, 2022-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Rotational anisotropy analysis of the lipid binding affinities for the wild type p85α BH domain and several p85α BH domain mutants ( A ) Changes in fluorescence polarization were measured for Bodipy-FL labelled phosphatidylinositol lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.

    Journal: Oncotarget

    Article Title: Insight into the PTEN – p85α interaction and lipid binding properties of the p85α BH domain

    doi: 10.18632/oncotarget.26432

    Figure Lengend Snippet: Rotational anisotropy analysis of the lipid binding affinities for the wild type p85α BH domain and several p85α BH domain mutants ( A ) Changes in fluorescence polarization were measured for Bodipy-FL labelled phosphatidylinositol lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.

    Article Snippet: Reaction mixtures (70 µl), contained Bodipy-FL labelled phosphatidylinositol lipids (50 nm; Echelon Biosciences Cat#: C-00F6, C-03F6, C45F6, and C39F6) in 50 mM Tris pH 7.0, 150 mM NaCl, 1 mM EDTA buffer.

    Techniques: Binding Assay, Fluorescence, Labeling, Mutagenesis