bodipy fl labelled phosphatidylinositol lipids  (Echelon Biosciences)


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    Echelon Biosciences bodipy fl labelled phosphatidylinositol lipids
    Rotational anisotropy analysis of the lipid binding affinities for the wild type p85α BH domain and several p85α BH domain mutants ( A ) Changes in fluorescence polarization were measured for <t>Bodipy-FL</t> labelled <t>phosphatidylinositol</t> lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.
    Bodipy Fl Labelled Phosphatidylinositol Lipids, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bodipy fl labelled phosphatidylinositol lipids/product/Echelon Biosciences
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bodipy fl labelled phosphatidylinositol lipids - by Bioz Stars, 2022-08
    90/100 stars

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    1) Product Images from "Insight into the PTEN – p85α interaction and lipid binding properties of the p85α BH domain"

    Article Title: Insight into the PTEN – p85α interaction and lipid binding properties of the p85α BH domain

    Journal: Oncotarget

    doi: 10.18632/oncotarget.26432

    Rotational anisotropy analysis of the lipid binding affinities for the wild type p85α BH domain and several p85α BH domain mutants ( A ) Changes in fluorescence polarization were measured for Bodipy-FL labelled phosphatidylinositol lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.
    Figure Legend Snippet: Rotational anisotropy analysis of the lipid binding affinities for the wild type p85α BH domain and several p85α BH domain mutants ( A ) Changes in fluorescence polarization were measured for Bodipy-FL labelled phosphatidylinositol lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.

    Techniques Used: Binding Assay, Fluorescence, Labeling, Mutagenesis

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    Echelon Biosciences bodipy fl labelled phosphatidylinositol lipids
    Rotational anisotropy analysis of the lipid binding affinities for the wild type p85α BH domain and several p85α BH domain mutants ( A ) Changes in fluorescence polarization were measured for <t>Bodipy-FL</t> labelled <t>phosphatidylinositol</t> lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.
    Bodipy Fl Labelled Phosphatidylinositol Lipids, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bodipy fl labelled phosphatidylinositol lipids/product/Echelon Biosciences
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bodipy fl labelled phosphatidylinositol lipids - by Bioz Stars, 2022-08
    90/100 stars
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    Rotational anisotropy analysis of the lipid binding affinities for the wild type p85α BH domain and several p85α BH domain mutants ( A ) Changes in fluorescence polarization were measured for Bodipy-FL labelled phosphatidylinositol lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.

    Journal: Oncotarget

    Article Title: Insight into the PTEN – p85α interaction and lipid binding properties of the p85α BH domain

    doi: 10.18632/oncotarget.26432

    Figure Lengend Snippet: Rotational anisotropy analysis of the lipid binding affinities for the wild type p85α BH domain and several p85α BH domain mutants ( A ) Changes in fluorescence polarization were measured for Bodipy-FL labelled phosphatidylinositol lipids: phosphatidylinositol (PI), phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4,5-bisphosphate (PI4,5P 2 ), and phosphatidylinositol 3,4,5-trisphosphate (PI3,4,5P 3 ), upon mixing with increasing concentrations of wild type p85α BH (105–319) protein. ( B–D ) Changes in fluorescence polarization for the indicated bodipy-labeled lipid were measured for the wild type or p85α BH (105–319) mutant proteins as in panel A; PI3P (B), PI4,5P 2 (C), PI3,4,5P 3 (D). Mean ± SEM is shown for each data point from 3 independent experiments.

    Article Snippet: Reaction mixtures (70 µl), contained Bodipy-FL labelled phosphatidylinositol lipids (50 nm; Echelon Biosciences Cat#: C-00F6, C-03F6, C45F6, and C39F6) in 50 mM Tris pH 7.0, 150 mM NaCl, 1 mM EDTA buffer.

    Techniques: Binding Assay, Fluorescence, Labeling, Mutagenesis