c tropicalis cells  (ATCC)


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    ATCC c tropicalis cells
    a , b Observed species; c , d Chao1 index; e , f PCoA based on unweighted UniFrac distance; g Relative abundance of fungi at the phylum level; h Relative abundance of Ascomycota; i Relative abundance of fungi at the species level; j Relative abundance of C. <t>tropicalis</t> . Data were either analyzed by two-tailed Student’s t-test or by factorial ANOVA. Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01. ns, not significant, n = 38 for diarrheal piglets ( n = 18 for diarrheal male, n = 20 for diarrheal female); n = 27 for control piglets ( n = 13 for control male and n = 14 for control female).
    C Tropicalis Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearance"

    Article Title: Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearance

    Journal: NPJ Biofilms and Microbiomes

    doi: 10.1038/s41522-023-00433-0

    a , b Observed species; c , d Chao1 index; e , f PCoA based on unweighted UniFrac distance; g Relative abundance of fungi at the phylum level; h Relative abundance of Ascomycota; i Relative abundance of fungi at the species level; j Relative abundance of C. tropicalis . Data were either analyzed by two-tailed Student’s t-test or by factorial ANOVA. Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01. ns, not significant, n = 38 for diarrheal piglets ( n = 18 for diarrheal male, n = 20 for diarrheal female); n = 27 for control piglets ( n = 13 for control male and n = 14 for control female).
    Figure Legend Snippet: a , b Observed species; c , d Chao1 index; e , f PCoA based on unweighted UniFrac distance; g Relative abundance of fungi at the phylum level; h Relative abundance of Ascomycota; i Relative abundance of fungi at the species level; j Relative abundance of C. tropicalis . Data were either analyzed by two-tailed Student’s t-test or by factorial ANOVA. Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01. ns, not significant, n = 38 for diarrheal piglets ( n = 18 for diarrheal male, n = 20 for diarrheal female); n = 27 for control piglets ( n = 13 for control male and n = 14 for control female).

    Techniques Used: Two Tailed Test

    a PCA plot (dark, healthy female; red, healthy male; green, diarrheal female; blue, diarrheal male); b Heatmap showing the changes of metabolites; c KEGG analysis revealing the affected pathways; d Relative abundance of choline and phosphocholine (The data were analyzed by factorial ANOVA; n = 18 for diarrheal male, n = 20 for diarrheal female, n = 13 for control male and n = 14 for control female.); e Correlation of C. tropocalis level with the abundance of choline and phosphocholine, as determined by Spearman correlation analyses. f Addition of different contents of phosphocholine on C. tropocalis growth. g The alteration of phosphocholine content in the culture medium with time (The data were analyzed with one-way ANOVA, n = 3). Weaned piglets were orally gavaged with PBS or with 1 × 10 8 C. tropicalis , and then C. tropicalis h and phosphocholine content i in the feces were determined at day 7; Weaned piglets were either fed a basal diet or a basal diet supplemented with 0.1% fluconazole, and then C. tropicalis j and phosphocholine content k in the feces were determined at day 7 (Data were analyzed by two-tailed Student’s t-test, n = 6). Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. ns not significant.
    Figure Legend Snippet: a PCA plot (dark, healthy female; red, healthy male; green, diarrheal female; blue, diarrheal male); b Heatmap showing the changes of metabolites; c KEGG analysis revealing the affected pathways; d Relative abundance of choline and phosphocholine (The data were analyzed by factorial ANOVA; n = 18 for diarrheal male, n = 20 for diarrheal female, n = 13 for control male and n = 14 for control female.); e Correlation of C. tropocalis level with the abundance of choline and phosphocholine, as determined by Spearman correlation analyses. f Addition of different contents of phosphocholine on C. tropocalis growth. g The alteration of phosphocholine content in the culture medium with time (The data were analyzed with one-way ANOVA, n = 3). Weaned piglets were orally gavaged with PBS or with 1 × 10 8 C. tropicalis , and then C. tropicalis h and phosphocholine content i in the feces were determined at day 7; Weaned piglets were either fed a basal diet or a basal diet supplemented with 0.1% fluconazole, and then C. tropicalis j and phosphocholine content k in the feces were determined at day 7 (Data were analyzed by two-tailed Student’s t-test, n = 6). Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. ns not significant.

    Techniques Used: Two Tailed Test

    a Relative abundance of C. tropocalis in the colonic mucosa of healthy control piglets relative to those of diarrheal piglets after normalization to total fungi (18 S) (Data were analyzed by two-tailed Student’s t-test, n = 10). b Hydrogen peroxide content in the colonic tissue; c , d SOD and GPX activity ( n = 6, Data were analyzed with one-way ANOVA and presented as mean ± SEM); e C. tropicalis cells were cultured in yeast extract-peptone-dextrose medium. The cell density was adjusted to an OD 600 of 1.0 and diluted to a 10 -5 dilution by 10-fold serial dilution. The cells were incubated at 37 °C and treated with different levels of hydrogen peroxide, and then the growth of C. tropicalis was assessed. Data were analyzed with one-way ANOVA and presented as mean ± SEM, n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001 ( C. tropicalis cells did not treat with hydrogen peroxide compared with cells treated with 1 mM or 2 mM hydrogen peroxide). f MPO activity in the colonic tissue; Pre-weaned piglets, samples were collected one day before weanling; Post-weaned C, samples were collected from healthy piglets three days after weanling; Post-weaned D, samples were collected from diarrheal piglets three days after weanling, n = 6. g Localization of neutrophil infiltration (MPO-positive, red) and NETs (Histone H2A, green) within the intestinal mucosa. Scale bar = 100 μm. h The MPO-DNA level in cell culture supernatant ( n = 4). i C. tropicalis survival ( C. tropicalis were co-cultured with hydrogen peroxide-treated neutrophils, n = 4). Data were analyzed with one-way ANOVA and presented as mean ± SEM. * P < 0.05, ** P < 0.01.
    Figure Legend Snippet: a Relative abundance of C. tropocalis in the colonic mucosa of healthy control piglets relative to those of diarrheal piglets after normalization to total fungi (18 S) (Data were analyzed by two-tailed Student’s t-test, n = 10). b Hydrogen peroxide content in the colonic tissue; c , d SOD and GPX activity ( n = 6, Data were analyzed with one-way ANOVA and presented as mean ± SEM); e C. tropicalis cells were cultured in yeast extract-peptone-dextrose medium. The cell density was adjusted to an OD 600 of 1.0 and diluted to a 10 -5 dilution by 10-fold serial dilution. The cells were incubated at 37 °C and treated with different levels of hydrogen peroxide, and then the growth of C. tropicalis was assessed. Data were analyzed with one-way ANOVA and presented as mean ± SEM, n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001 ( C. tropicalis cells did not treat with hydrogen peroxide compared with cells treated with 1 mM or 2 mM hydrogen peroxide). f MPO activity in the colonic tissue; Pre-weaned piglets, samples were collected one day before weanling; Post-weaned C, samples were collected from healthy piglets three days after weanling; Post-weaned D, samples were collected from diarrheal piglets three days after weanling, n = 6. g Localization of neutrophil infiltration (MPO-positive, red) and NETs (Histone H2A, green) within the intestinal mucosa. Scale bar = 100 μm. h The MPO-DNA level in cell culture supernatant ( n = 4). i C. tropicalis survival ( C. tropicalis were co-cultured with hydrogen peroxide-treated neutrophils, n = 4). Data were analyzed with one-way ANOVA and presented as mean ± SEM. * P < 0.05, ** P < 0.01.

    Techniques Used: Two Tailed Test, Activity Assay, Cell Culture, Serial Dilution, Incubation

    Relative dectin-1 expression a and MPO-DNA complex ratio b in neutrophils from wild-type mice after treated with 0.02 mM hydrogen peroxide; HP, hydrogen peroxide. Data were analyzed using a two-tailed Student’s t-test and are presented as the mean ± SEM. n = 4. * P < 0.05, ** P < 0.01. Relative ROS content c and dectin-1 gene expression d in the colonic tissue of mice; e Localization of neutrophil infiltration (MPO-positive, green) and NETs (Cit-H3, red) within the intestinal mucosa (Scale bar = 50 μm); f Relative abundance of C. tropicalis in the colonic mucosa ; g Phosphocholine content in the feces; h Fecal water content; i Fecal Na + content; j Colonic histomorphology (Scale bar = 200 μm). WT wild-type mice, KO dectin-1 knockout mice. Dectin-1 knockout and wild-type mice were instilled with 0.2 mL hydrogen peroxide solution in PBS (0.02 mM) via the intrarectal route once a day for 1 week. Data were analyzed using one-way analysis of variance (ANOVA) and presented as the mean ± SEM. n = 6. * P < 0.05, ** P < 0.01.
    Figure Legend Snippet: Relative dectin-1 expression a and MPO-DNA complex ratio b in neutrophils from wild-type mice after treated with 0.02 mM hydrogen peroxide; HP, hydrogen peroxide. Data were analyzed using a two-tailed Student’s t-test and are presented as the mean ± SEM. n = 4. * P < 0.05, ** P < 0.01. Relative ROS content c and dectin-1 gene expression d in the colonic tissue of mice; e Localization of neutrophil infiltration (MPO-positive, green) and NETs (Cit-H3, red) within the intestinal mucosa (Scale bar = 50 μm); f Relative abundance of C. tropicalis in the colonic mucosa ; g Phosphocholine content in the feces; h Fecal water content; i Fecal Na + content; j Colonic histomorphology (Scale bar = 200 μm). WT wild-type mice, KO dectin-1 knockout mice. Dectin-1 knockout and wild-type mice were instilled with 0.2 mL hydrogen peroxide solution in PBS (0.02 mM) via the intrarectal route once a day for 1 week. Data were analyzed using one-way analysis of variance (ANOVA) and presented as the mean ± SEM. n = 6. * P < 0.05, ** P < 0.01.

    Techniques Used: Expressing, Two Tailed Test, Knock-Out

    c tropicalis atcc 7349  (ATCC)


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    ATCC c tropicalis atcc 7349
    Antifungal susceptibility of DL and AmB against Candida species
    C Tropicalis Atcc 7349, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Dehydrocostus lactone inhibits Candida albicans growth and biofilm formation"

    Article Title: Dehydrocostus lactone inhibits Candida albicans growth and biofilm formation

    Journal: AMB Express

    doi: 10.1186/s13568-023-01587-y

    Antifungal susceptibility of DL and AmB against Candida species
    Figure Legend Snippet: Antifungal susceptibility of DL and AmB against Candida species

    Techniques Used:

    c tropicalis ci  (ATCC)


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    ATCC c tropicalis ci
    Disk diffusion test results of OOEt (inhibition zone diameters in mm).
    C Tropicalis Ci, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites"

    Article Title: Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites

    Journal: Microorganisms

    doi: 10.3390/microorganisms11081987

    Disk diffusion test results of OOEt (inhibition zone diameters in mm).
    Figure Legend Snippet: Disk diffusion test results of OOEt (inhibition zone diameters in mm).

    Techniques Used: Diffusion-based Assay, Inhibition

    Pearson correlation test results and 95% confidence interval for the differences of means for the disk diffusion test results of OOEt.
    Figure Legend Snippet: Pearson correlation test results and 95% confidence interval for the differences of means for the disk diffusion test results of OOEt.

    Techniques Used: Diffusion-based Assay

    Minimum inhibitory concentration (MIC) test results.
    Figure Legend Snippet: Minimum inhibitory concentration (MIC) test results.

    Techniques Used: Concentration Assay

    c tropicalis  (ATCC)


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    ATCC c tropicalis
    Antifungal efficiency of the greenly synthesized ZnO-NPs against candidal pathogens.
    C Tropicalis, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Synergistic Anticandidal Effectiveness of Greenly Synthesized Zinc Oxide Nanoparticles with Antifungal Agents against Nosocomial Candidal Pathogens"

    Article Title: Synergistic Anticandidal Effectiveness of Greenly Synthesized Zinc Oxide Nanoparticles with Antifungal Agents against Nosocomial Candidal Pathogens

    Journal: Microorganisms

    doi: 10.3390/microorganisms11081957

    Antifungal efficiency of the greenly synthesized ZnO-NPs against candidal pathogens.
    Figure Legend Snippet: Antifungal efficiency of the greenly synthesized ZnO-NPs against candidal pathogens.

    Techniques Used: Synthesized, Inhibition, Negative Control

    ( a ) Morphological deformations of C. albicans treated with ZnO-NPs (the morphological distortions of treated C. albicans cells are pointed out by arrows). ( b ) Morphological deformations of C. tropicalis treated with ZnO-NPs (the deformations of treated C. tropicalis cells are pointed out by arrows).
    Figure Legend Snippet: ( a ) Morphological deformations of C. albicans treated with ZnO-NPs (the morphological distortions of treated C. albicans cells are pointed out by arrows). ( b ) Morphological deformations of C. tropicalis treated with ZnO-NPs (the deformations of treated C. tropicalis cells are pointed out by arrows).

    Techniques Used:

    The fractional inhibitory concentration index (FICI) of the combined action of biogenic ZnO-NPs and antifungal agents.
    Figure Legend Snippet: The fractional inhibitory concentration index (FICI) of the combined action of biogenic ZnO-NPs and antifungal agents.

    Techniques Used: Concentration Assay

    c tropicalis  (ATCC)


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    ATCC c tropicalis
    The anti-candida activities of C. citratus (The total growth area mm).
    C Tropicalis, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Biological Activity of Biosynthesized Silver Nanoaggregates Prepared from the Aqueous Extract of Cymbopogon citratus against Candida spp."

    Article Title: Biological Activity of Biosynthesized Silver Nanoaggregates Prepared from the Aqueous Extract of Cymbopogon citratus against Candida spp.

    Journal: Nanomaterials

    doi: 10.3390/nano13152198

    The anti-candida activities of C. citratus (The total growth area mm).
    Figure Legend Snippet: The anti-candida activities of C. citratus (The total growth area mm).

    Techniques Used: Negative Control

    c tropicalis atcc 750  (ATCC)


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    ATCC c tropicalis atcc 750
    C Tropicalis Atcc 750, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    c tropicalis atcc 750  (ATCC)


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    ATCC c tropicalis atcc 750
    C Tropicalis Atcc 750, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    c tropicalis atcc 750  (ATCC)


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    ATCC c tropicalis atcc 750
    C Tropicalis Atcc 750, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    c tropicalis atcc 750  (ATCC)


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    ATCC c tropicalis atcc 750
    C Tropicalis Atcc 750, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    c tropicalis dsm 1346  (ATCC)


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    ATCC c tropicalis dsm 1346
    Antimicrobial activity reported for Lentinus crinitus (L.) Fr (Polyporales, Basidiomycota).
    C Tropicalis Dsm 1346, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Probiotic growth-stimulating capacity and antimicrobial activities of aqueous extracts of Lentinus crinitus (L.) Fr (polyporales, basidiomycota) "

    Article Title: Probiotic growth-stimulating capacity and antimicrobial activities of aqueous extracts of Lentinus crinitus (L.) Fr (polyporales, basidiomycota)

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2023.e18738


    Figure Legend Snippet: Antimicrobial activity reported for Lentinus crinitus (L.) Fr (Polyporales, Basidiomycota).

    Techniques Used: Activity Assay

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    ATCC c tropicalis cells
    a , b Observed species; c , d Chao1 index; e , f PCoA based on unweighted UniFrac distance; g Relative abundance of fungi at the phylum level; h Relative abundance of Ascomycota; i Relative abundance of fungi at the species level; j Relative abundance of C. <t>tropicalis</t> . Data were either analyzed by two-tailed Student’s t-test or by factorial ANOVA. Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01. ns, not significant, n = 38 for diarrheal piglets ( n = 18 for diarrheal male, n = 20 for diarrheal female); n = 27 for control piglets ( n = 13 for control male and n = 14 for control female).
    C Tropicalis Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC c tropicalis atcc 7349
    Antifungal susceptibility of DL and AmB against Candida species
    C Tropicalis Atcc 7349, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC c tropicalis ci
    Disk diffusion test results of OOEt (inhibition zone diameters in mm).
    C Tropicalis Ci, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC c tropicalis
    Antifungal efficiency of the greenly synthesized ZnO-NPs against candidal pathogens.
    C Tropicalis, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC c tropicalis atcc 750
    Antifungal efficiency of the greenly synthesized ZnO-NPs against candidal pathogens.
    C Tropicalis Atcc 750, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC c tropicalis dsm 1346
    Antimicrobial activity reported for Lentinus crinitus (L.) Fr (Polyporales, Basidiomycota).
    C Tropicalis Dsm 1346, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a , b Observed species; c , d Chao1 index; e , f PCoA based on unweighted UniFrac distance; g Relative abundance of fungi at the phylum level; h Relative abundance of Ascomycota; i Relative abundance of fungi at the species level; j Relative abundance of C. tropicalis . Data were either analyzed by two-tailed Student’s t-test or by factorial ANOVA. Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01. ns, not significant, n = 38 for diarrheal piglets ( n = 18 for diarrheal male, n = 20 for diarrheal female); n = 27 for control piglets ( n = 13 for control male and n = 14 for control female).

    Journal: NPJ Biofilms and Microbiomes

    Article Title: Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearance

    doi: 10.1038/s41522-023-00433-0

    Figure Lengend Snippet: a , b Observed species; c , d Chao1 index; e , f PCoA based on unweighted UniFrac distance; g Relative abundance of fungi at the phylum level; h Relative abundance of Ascomycota; i Relative abundance of fungi at the species level; j Relative abundance of C. tropicalis . Data were either analyzed by two-tailed Student’s t-test or by factorial ANOVA. Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01. ns, not significant, n = 38 for diarrheal piglets ( n = 18 for diarrheal male, n = 20 for diarrheal female); n = 27 for control piglets ( n = 13 for control male and n = 14 for control female).

    Article Snippet: C. tropicalis cells (ATCC 20026) were cultured in a yeast extract-peptone-dextrose medium.

    Techniques: Two Tailed Test

    a PCA plot (dark, healthy female; red, healthy male; green, diarrheal female; blue, diarrheal male); b Heatmap showing the changes of metabolites; c KEGG analysis revealing the affected pathways; d Relative abundance of choline and phosphocholine (The data were analyzed by factorial ANOVA; n = 18 for diarrheal male, n = 20 for diarrheal female, n = 13 for control male and n = 14 for control female.); e Correlation of C. tropocalis level with the abundance of choline and phosphocholine, as determined by Spearman correlation analyses. f Addition of different contents of phosphocholine on C. tropocalis growth. g The alteration of phosphocholine content in the culture medium with time (The data were analyzed with one-way ANOVA, n = 3). Weaned piglets were orally gavaged with PBS or with 1 × 10 8 C. tropicalis , and then C. tropicalis h and phosphocholine content i in the feces were determined at day 7; Weaned piglets were either fed a basal diet or a basal diet supplemented with 0.1% fluconazole, and then C. tropicalis j and phosphocholine content k in the feces were determined at day 7 (Data were analyzed by two-tailed Student’s t-test, n = 6). Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. ns not significant.

    Journal: NPJ Biofilms and Microbiomes

    Article Title: Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearance

    doi: 10.1038/s41522-023-00433-0

    Figure Lengend Snippet: a PCA plot (dark, healthy female; red, healthy male; green, diarrheal female; blue, diarrheal male); b Heatmap showing the changes of metabolites; c KEGG analysis revealing the affected pathways; d Relative abundance of choline and phosphocholine (The data were analyzed by factorial ANOVA; n = 18 for diarrheal male, n = 20 for diarrheal female, n = 13 for control male and n = 14 for control female.); e Correlation of C. tropocalis level with the abundance of choline and phosphocholine, as determined by Spearman correlation analyses. f Addition of different contents of phosphocholine on C. tropocalis growth. g The alteration of phosphocholine content in the culture medium with time (The data were analyzed with one-way ANOVA, n = 3). Weaned piglets were orally gavaged with PBS or with 1 × 10 8 C. tropicalis , and then C. tropicalis h and phosphocholine content i in the feces were determined at day 7; Weaned piglets were either fed a basal diet or a basal diet supplemented with 0.1% fluconazole, and then C. tropicalis j and phosphocholine content k in the feces were determined at day 7 (Data were analyzed by two-tailed Student’s t-test, n = 6). Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. ns not significant.

    Article Snippet: C. tropicalis cells (ATCC 20026) were cultured in a yeast extract-peptone-dextrose medium.

    Techniques: Two Tailed Test

    a Relative abundance of C. tropocalis in the colonic mucosa of healthy control piglets relative to those of diarrheal piglets after normalization to total fungi (18 S) (Data were analyzed by two-tailed Student’s t-test, n = 10). b Hydrogen peroxide content in the colonic tissue; c , d SOD and GPX activity ( n = 6, Data were analyzed with one-way ANOVA and presented as mean ± SEM); e C. tropicalis cells were cultured in yeast extract-peptone-dextrose medium. The cell density was adjusted to an OD 600 of 1.0 and diluted to a 10 -5 dilution by 10-fold serial dilution. The cells were incubated at 37 °C and treated with different levels of hydrogen peroxide, and then the growth of C. tropicalis was assessed. Data were analyzed with one-way ANOVA and presented as mean ± SEM, n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001 ( C. tropicalis cells did not treat with hydrogen peroxide compared with cells treated with 1 mM or 2 mM hydrogen peroxide). f MPO activity in the colonic tissue; Pre-weaned piglets, samples were collected one day before weanling; Post-weaned C, samples were collected from healthy piglets three days after weanling; Post-weaned D, samples were collected from diarrheal piglets three days after weanling, n = 6. g Localization of neutrophil infiltration (MPO-positive, red) and NETs (Histone H2A, green) within the intestinal mucosa. Scale bar = 100 μm. h The MPO-DNA level in cell culture supernatant ( n = 4). i C. tropicalis survival ( C. tropicalis were co-cultured with hydrogen peroxide-treated neutrophils, n = 4). Data were analyzed with one-way ANOVA and presented as mean ± SEM. * P < 0.05, ** P < 0.01.

    Journal: NPJ Biofilms and Microbiomes

    Article Title: Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearance

    doi: 10.1038/s41522-023-00433-0

    Figure Lengend Snippet: a Relative abundance of C. tropocalis in the colonic mucosa of healthy control piglets relative to those of diarrheal piglets after normalization to total fungi (18 S) (Data were analyzed by two-tailed Student’s t-test, n = 10). b Hydrogen peroxide content in the colonic tissue; c , d SOD and GPX activity ( n = 6, Data were analyzed with one-way ANOVA and presented as mean ± SEM); e C. tropicalis cells were cultured in yeast extract-peptone-dextrose medium. The cell density was adjusted to an OD 600 of 1.0 and diluted to a 10 -5 dilution by 10-fold serial dilution. The cells were incubated at 37 °C and treated with different levels of hydrogen peroxide, and then the growth of C. tropicalis was assessed. Data were analyzed with one-way ANOVA and presented as mean ± SEM, n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001 ( C. tropicalis cells did not treat with hydrogen peroxide compared with cells treated with 1 mM or 2 mM hydrogen peroxide). f MPO activity in the colonic tissue; Pre-weaned piglets, samples were collected one day before weanling; Post-weaned C, samples were collected from healthy piglets three days after weanling; Post-weaned D, samples were collected from diarrheal piglets three days after weanling, n = 6. g Localization of neutrophil infiltration (MPO-positive, red) and NETs (Histone H2A, green) within the intestinal mucosa. Scale bar = 100 μm. h The MPO-DNA level in cell culture supernatant ( n = 4). i C. tropicalis survival ( C. tropicalis were co-cultured with hydrogen peroxide-treated neutrophils, n = 4). Data were analyzed with one-way ANOVA and presented as mean ± SEM. * P < 0.05, ** P < 0.01.

    Article Snippet: C. tropicalis cells (ATCC 20026) were cultured in a yeast extract-peptone-dextrose medium.

    Techniques: Two Tailed Test, Activity Assay, Cell Culture, Serial Dilution, Incubation

    Relative dectin-1 expression a and MPO-DNA complex ratio b in neutrophils from wild-type mice after treated with 0.02 mM hydrogen peroxide; HP, hydrogen peroxide. Data were analyzed using a two-tailed Student’s t-test and are presented as the mean ± SEM. n = 4. * P < 0.05, ** P < 0.01. Relative ROS content c and dectin-1 gene expression d in the colonic tissue of mice; e Localization of neutrophil infiltration (MPO-positive, green) and NETs (Cit-H3, red) within the intestinal mucosa (Scale bar = 50 μm); f Relative abundance of C. tropicalis in the colonic mucosa ; g Phosphocholine content in the feces; h Fecal water content; i Fecal Na + content; j Colonic histomorphology (Scale bar = 200 μm). WT wild-type mice, KO dectin-1 knockout mice. Dectin-1 knockout and wild-type mice were instilled with 0.2 mL hydrogen peroxide solution in PBS (0.02 mM) via the intrarectal route once a day for 1 week. Data were analyzed using one-way analysis of variance (ANOVA) and presented as the mean ± SEM. n = 6. * P < 0.05, ** P < 0.01.

    Journal: NPJ Biofilms and Microbiomes

    Article Title: Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearance

    doi: 10.1038/s41522-023-00433-0

    Figure Lengend Snippet: Relative dectin-1 expression a and MPO-DNA complex ratio b in neutrophils from wild-type mice after treated with 0.02 mM hydrogen peroxide; HP, hydrogen peroxide. Data were analyzed using a two-tailed Student’s t-test and are presented as the mean ± SEM. n = 4. * P < 0.05, ** P < 0.01. Relative ROS content c and dectin-1 gene expression d in the colonic tissue of mice; e Localization of neutrophil infiltration (MPO-positive, green) and NETs (Cit-H3, red) within the intestinal mucosa (Scale bar = 50 μm); f Relative abundance of C. tropicalis in the colonic mucosa ; g Phosphocholine content in the feces; h Fecal water content; i Fecal Na + content; j Colonic histomorphology (Scale bar = 200 μm). WT wild-type mice, KO dectin-1 knockout mice. Dectin-1 knockout and wild-type mice were instilled with 0.2 mL hydrogen peroxide solution in PBS (0.02 mM) via the intrarectal route once a day for 1 week. Data were analyzed using one-way analysis of variance (ANOVA) and presented as the mean ± SEM. n = 6. * P < 0.05, ** P < 0.01.

    Article Snippet: C. tropicalis cells (ATCC 20026) were cultured in a yeast extract-peptone-dextrose medium.

    Techniques: Expressing, Two Tailed Test, Knock-Out

    Antifungal susceptibility of DL and AmB against Candida species

    Journal: AMB Express

    Article Title: Dehydrocostus lactone inhibits Candida albicans growth and biofilm formation

    doi: 10.1186/s13568-023-01587-y

    Figure Lengend Snippet: Antifungal susceptibility of DL and AmB against Candida species

    Article Snippet: C. tropicalis ATCC 7349 , 64 , 128 , 1 , 2.

    Techniques:

    Disk diffusion test results of OOEt (inhibition zone diameters in mm).

    Journal: Microorganisms

    Article Title: Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites

    doi: 10.3390/microorganisms11081987

    Figure Lengend Snippet: Disk diffusion test results of OOEt (inhibition zone diameters in mm).

    Article Snippet: On the other hand, higher MIC values of 34.3 mg/mL were observed for S. enteritidis ATCC 13076, S. typhimurium SL 1344, K. pneumoniae (FI), S. boydii (CI), C. tropicalis (CI), E. coli (MDR), and K. pneumoniae (MDR) when treated with OOEt.

    Techniques: Diffusion-based Assay, Inhibition

    Pearson correlation test results and 95% confidence interval for the differences of means for the disk diffusion test results of OOEt.

    Journal: Microorganisms

    Article Title: Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites

    doi: 10.3390/microorganisms11081987

    Figure Lengend Snippet: Pearson correlation test results and 95% confidence interval for the differences of means for the disk diffusion test results of OOEt.

    Article Snippet: On the other hand, higher MIC values of 34.3 mg/mL were observed for S. enteritidis ATCC 13076, S. typhimurium SL 1344, K. pneumoniae (FI), S. boydii (CI), C. tropicalis (CI), E. coli (MDR), and K. pneumoniae (MDR) when treated with OOEt.

    Techniques: Diffusion-based Assay

    Minimum inhibitory concentration (MIC) test results.

    Journal: Microorganisms

    Article Title: Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites

    doi: 10.3390/microorganisms11081987

    Figure Lengend Snippet: Minimum inhibitory concentration (MIC) test results.

    Article Snippet: On the other hand, higher MIC values of 34.3 mg/mL were observed for S. enteritidis ATCC 13076, S. typhimurium SL 1344, K. pneumoniae (FI), S. boydii (CI), C. tropicalis (CI), E. coli (MDR), and K. pneumoniae (MDR) when treated with OOEt.

    Techniques: Concentration Assay

    Antifungal efficiency of the greenly synthesized ZnO-NPs against candidal pathogens.

    Journal: Microorganisms

    Article Title: Synergistic Anticandidal Effectiveness of Greenly Synthesized Zinc Oxide Nanoparticles with Antifungal Agents against Nosocomial Candidal Pathogens

    doi: 10.3390/microorganisms11081957

    Figure Lengend Snippet: Antifungal efficiency of the greenly synthesized ZnO-NPs against candidal pathogens.

    Article Snippet: The susceptibility of three strains of Candida , namely, C. albicans (ATCC 29213), C. glabrata (ATCC 25922), and C. tropicalis (ATCC 33592), to the biogenic ZnO-NPs was evaluated.

    Techniques: Synthesized, Inhibition, Negative Control

    ( a ) Morphological deformations of C. albicans treated with ZnO-NPs (the morphological distortions of treated C. albicans cells are pointed out by arrows). ( b ) Morphological deformations of C. tropicalis treated with ZnO-NPs (the deformations of treated C. tropicalis cells are pointed out by arrows).

    Journal: Microorganisms

    Article Title: Synergistic Anticandidal Effectiveness of Greenly Synthesized Zinc Oxide Nanoparticles with Antifungal Agents against Nosocomial Candidal Pathogens

    doi: 10.3390/microorganisms11081957

    Figure Lengend Snippet: ( a ) Morphological deformations of C. albicans treated with ZnO-NPs (the morphological distortions of treated C. albicans cells are pointed out by arrows). ( b ) Morphological deformations of C. tropicalis treated with ZnO-NPs (the deformations of treated C. tropicalis cells are pointed out by arrows).

    Article Snippet: The susceptibility of three strains of Candida , namely, C. albicans (ATCC 29213), C. glabrata (ATCC 25922), and C. tropicalis (ATCC 33592), to the biogenic ZnO-NPs was evaluated.

    Techniques:

    The fractional inhibitory concentration index (FICI) of the combined action of biogenic ZnO-NPs and antifungal agents.

    Journal: Microorganisms

    Article Title: Synergistic Anticandidal Effectiveness of Greenly Synthesized Zinc Oxide Nanoparticles with Antifungal Agents against Nosocomial Candidal Pathogens

    doi: 10.3390/microorganisms11081957

    Figure Lengend Snippet: The fractional inhibitory concentration index (FICI) of the combined action of biogenic ZnO-NPs and antifungal agents.

    Article Snippet: The susceptibility of three strains of Candida , namely, C. albicans (ATCC 29213), C. glabrata (ATCC 25922), and C. tropicalis (ATCC 33592), to the biogenic ZnO-NPs was evaluated.

    Techniques: Concentration Assay

    Journal: Heliyon

    Article Title: Probiotic growth-stimulating capacity and antimicrobial activities of aqueous extracts of Lentinus crinitus (L.) Fr (polyporales, basidiomycota)

    doi: 10.1016/j.heliyon.2023.e18738

    Figure Lengend Snippet: Antimicrobial activity reported for Lentinus crinitus (L.) Fr (Polyporales, Basidiomycota).

    Article Snippet: Culture broth/Submerged Fermentation , Crude extract with ethyl acetate. Most active compounds: 1-desoxy-hypnophilin and 6,7 epoxy-4 (15)-hirsutene-5-ol , Antimicrobial activity against: Bacillus cereus DSM 318, Staphylococcus aureus ATCC 13709, Escherichia coli ATCC 9637, Candida albicans ATCC 10231, C. tropicalis DSM 1346, Rhodotorula glutinis DSM 70398 , Ethiopia , 1994 , [ ] .

    Techniques: Activity Assay