Journal: PLoS ONE

Article Title: Feedback Regulations of miR-21 and MAPKs via Pdcd4 and Spry1 Are Involved in Arsenite-Induced Cell Malignant Transformation

doi: 10.1371/journal.pone.0057652

Figure Lengend Snippet: Densities of bands were quantified by Eagle Eye II software. GAPDH levels, measured in parallel, served as controls. Arsenite-transformed HELF cells were transfected with 50 nM of control siRNA, NF-κB p65 siRNA, or c-Jun siRNA for 24 h. (A) Western blots and (B) relative protein levels (means ± SD, n = 3) of NF-κB p65 and c-Jun. (C) qRT-PCR analysis of miR-21 expression (means ± SD, n = 3). ** P <0.01 difference from Con siRNA group. (D) Western blots (upper) and RT-PCR (below) analyses for protein and mRNA levels and (E) relative protein levels (means ± SD, n = 3) of Pdcd4, Spry1, cleaved-caspase-3, and caspase-3, ** P <0.01 difference from the control (Con) siRNA group.

Article Snippet: Control siRNA, NF-κB p65 siRNA, and c-Jun siRNA were purchased from Cell Signaling Technology.

Techniques: Software, Transformation Assay, Transfection, Western Blot, Quantitative RT-PCR, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: Interaction between c-jun and Androgen Receptor Determines the Outcome of Taxane Therapy in Castration Resistant Prostate Cancer

doi: 10.1371/journal.pone.0079573

Figure Lengend Snippet: (A) Western blotting analysis showing transfection efficiency of siRNA c-jun in both PC-3 and LNCaP cells. (B) Cell proliferation in LNCaP (upper panel) and PC-3 (lower panel) cells transfected with c-jun siRNA or nontargeted (control) vector. Cells were treated with Doc for 24 and 48 hours. LNCaP cells harbor c-jun siRNA showed a significant decrease in proliferation (p=0.002) while PC-3 cells were not affected.

Article Snippet: For siRNA experiments, PC-3 and LNCaP cells were transfected for 48 hours with 100 nM siRNA c-jun or non-targeting siRNA (Cell Signaling Technology, Danvers, MA, USA).

Techniques: Western Blot, Transfection, Plasmid Preparation