Journal:

Article Title: Effect of 4-trifluoromethyl derivatives of salicylate on nuclear factor κ B-dependent transcription in human astrocytoma cells

doi: 10.1038/sj.bjp.0703820

Figure Lengend Snippet: Effect of HTB on the activation of JNK and p38-MAP kinase tyrosine phosphorylation. 1321N1 cells were incubated with 100 u ml−1 TNF-α for 15 min, in the presence and absence of 3 mM HTB. At the end of this period cell lysates were collected for the assay of P-GST-c-Jun phosphorylation with phosphospecific anti-c-Jun antibody. This is a representative experiment of three identical ones (upper panel). The lower panel shows a typical experiment carried out under identical conditions, in which cell lysates were used for immunoprecipitation with anti-p38-antibody, SDS – PAGE separation of the immunoprecipitate, and blotting with antiphosphotyrosine antibody. P-Y, phosphotyrosine.

Article Snippet: Phosphorylated GST-IκB was resolved by 10% SDS – PAGE and detected with the Bio-Rad Molecular Imager FX system. c-Jun N-terminal kinase was assayed using GST-c-Jun fusion protein as the substrate.

Techniques: Activation Assay, Incubation, Immunoprecipitation, SDS Page