Journal: Foods

Article Title: Aerotolerance and Multi-Locus Sequence Typing of Campylobacter jejuni Isolated from Commercial Broiler Processing Plants

doi: 10.3390/foods12173305

Figure Lengend Snippet: Sources and number of C. jejuni isolates from different steps of commercial broiler processing plants.

Article Snippet: The housekeeping genes amplified from genomic DNA from C. jejuni ATCC 29428 were used as a positive control.

Techniques:

Journal: Foods

Article Title: Aerotolerance and Multi-Locus Sequence Typing of Campylobacter jejuni Isolated from Commercial Broiler Processing Plants

doi: 10.3390/foods12173305

Figure Lengend Snippet: Average Log CFU/ml of C. jejuni isolates at 24 h when incubated micro-aerobically at 42 °C in BHI broth with shaking at 200 rpm. The results show the mean and standard deviation of the three independent replicates.

Article Snippet: The housekeeping genes amplified from genomic DNA from C. jejuni ATCC 29428 were used as a positive control.

Techniques: Incubation, Standard Deviation

Journal: Foods

Article Title: Aerotolerance and Multi-Locus Sequence Typing of Campylobacter jejuni Isolated from Commercial Broiler Processing Plants

doi: 10.3390/foods12173305

Figure Lengend Snippet: Average Log CFU/ml of aero-sensitive C. jejuni isolates at 0 h, 12 h, and 24 h time points in an aerobic condition. The results show the mean and standard deviation of the three independent replicates. These isolates did not survive 12 h and 24 h of aerobic exposure.

Article Snippet: The housekeeping genes amplified from genomic DNA from C. jejuni ATCC 29428 were used as a positive control.

Techniques: Standard Deviation

Journal: Foods

Article Title: Aerotolerance and Multi-Locus Sequence Typing of Campylobacter jejuni Isolated from Commercial Broiler Processing Plants

doi: 10.3390/foods12173305

Figure Lengend Snippet: Average Log CFU/ml of intermediate aerotolerant C. jejuni isolates at 0 h, 12 h, and 24 h time points in an aerobic condition. These isolates did not survive 24 h of aerobic exposure. The results show the mean and standard deviation of the three independent replicates.

Article Snippet: The housekeeping genes amplified from genomic DNA from C. jejuni ATCC 29428 were used as a positive control.

Techniques: Standard Deviation

Journal: Foods

Article Title: Aerotolerance and Multi-Locus Sequence Typing of Campylobacter jejuni Isolated from Commercial Broiler Processing Plants

doi: 10.3390/foods12173305

Figure Lengend Snippet: Average Log CFU/ml of hyper aerotolerant C. jejuni isolates at 0 h, 12 h, and 24 h time points in an aerobic condition. The results show the mean and standard deviation of the three independent replicates.

Article Snippet: The housekeeping genes amplified from genomic DNA from C. jejuni ATCC 29428 were used as a positive control.

Techniques: Standard Deviation

Journal: Foods

Article Title: Aerotolerance and Multi-Locus Sequence Typing of Campylobacter jejuni Isolated from Commercial Broiler Processing Plants

doi: 10.3390/foods12173305

Figure Lengend Snippet: Frequency distribution of C. jejuni sequence type (ST) and clonal complexes (CC) among isolates with different aerotolerance levels. AS = Aero-sensitive; IAT = intermediate aerotolerant; HAT = Hyper aerotolerant; UN = Unassigned, not assigned to any clonal complexes.

Article Snippet: The housekeeping genes amplified from genomic DNA from C. jejuni ATCC 29428 were used as a positive control.

Techniques: Sequencing

Journal: Foods

Article Title: Aerotolerance and Multi-Locus Sequence Typing of Campylobacter jejuni Isolated from Commercial Broiler Processing Plants

doi: 10.3390/foods12173305

Figure Lengend Snippet: Reduction in C. jejuni counts when stored at 4 °C for 7 days ( p < 0.001). Means with different letters are statistically different. The error bar represents the pooled standard error of the means.

Article Snippet: The housekeeping genes amplified from genomic DNA from C. jejuni ATCC 29428 were used as a positive control.

Techniques:

Journal: Foods

Article Title: Aerotolerance and Multi-Locus Sequence Typing of Campylobacter jejuni Isolated from Commercial Broiler Processing Plants

doi: 10.3390/foods12173305

Figure Lengend Snippet: Reduction in C. jejuni counts when stored at −20 °C for 14 days ( p < 0.001). Means with different letters are statistically different. The error bar represents the pooled standard error of the means.

Article Snippet: The housekeeping genes amplified from genomic DNA from C. jejuni ATCC 29428 were used as a positive control.

Techniques:

Journal: Microorganisms

Article Title: First Report of Campylobacter jejuni Strains Belonging to ST-21 Clonal Complex Isolated from Human, Poultry and Wild Birds in Croatia: Antimicrobial Resistance and Genetic Distance

doi: 10.3390/microorganisms11081884

Figure Lengend Snippet: Minimum spanning tree (MST) revealing genomic relationship amongst C. jejuni ST-21 CC based on cgMLST profiles. Different colors represent different reservoirs of strains. The numbers next to the samples correlate with the identified STs. The asterisk mark indicates clusters that are phenotypically defined as fluoroquinolones wild-type strains.

Article Snippet: For quality control of susceptibility, to ensure that the results were within acceptable limits, the C. jejuni ATCC 33,560 reference strain was used.

Techniques:

Journal: Microbiology Spectrum

Article Title: Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants

doi: 10.1128/spectrum.01070-23

Figure Lengend Snippet: Clinical and phenotypical data of the Campylobacter jejuni studied isolates ^a

Article Snippet: Locus tag of the C. jejuni subsp. jejuni NCTC 11168 = ATCC 700819 strain (GenBank NC_002163.1 ) was adopted to designate all gene hits identified in the subsequent analyses, whenever it was possible.

Techniques: Isolation

Journal: Microbiology Spectrum

Article Title: Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants

doi: 10.1128/spectrum.01070-23

Figure Lengend Snippet: Treatment timeline of the patients from case A and case B recurrent infections by Campylobacter jejuni . For both cases, bacterial strains isolated from blood or fecal samples are indicated in full and dotted lines, respectively, while the antibiotics used and their duration are shown in colored bold horizontal lines (each bullet corresponds to a week). AZM, azithromycin; ETP, ertapenem; MEM, meropenem; GEN, gentamicin; NEO, neomycin; CFM, Cefixime; CHL, chloramphenicol; TGC, tigecycline.

Article Snippet: Locus tag of the C. jejuni subsp. jejuni NCTC 11168 = ATCC 700819 strain (GenBank NC_002163.1 ) was adopted to designate all gene hits identified in the subsequent analyses, whenever it was possible.

Techniques: Isolation

Journal: Microbiology Spectrum

Article Title: Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants

doi: 10.1128/spectrum.01070-23

Figure Lengend Snippet: Known and putative genetic determinants of antibiotic resistance in Campylobacter jejuni strains recovered from two cases of recurrent infections. (n), number of copies; *, allele mixture; **, MIC 10× higher than other susceptible strains (see Table 1 ); ***, heterogeneous resistance; †, restores TATA box from GAAAAT to TAAAAT in the -10 promoter region; ††, restores the Campylobacter jejuni ribosome binding site (RBS) consensus sequence (AAGGA); †††, affects the Campylobacter jejuni -35 consensus sequence (TTTAAGTnTT); ††††, leads to the loss of a second RBS.

Article Snippet: Locus tag of the C. jejuni subsp. jejuni NCTC 11168 = ATCC 700819 strain (GenBank NC_002163.1 ) was adopted to designate all gene hits identified in the subsequent analyses, whenever it was possible.

Techniques: Binding Assay, Sequencing

Journal: Microbiology Spectrum

Article Title: Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants

doi: 10.1128/spectrum.01070-23

Figure Lengend Snippet: In silico predicted MOMP 3D structure for Campylobacter jejuni isolates from clinical case A and case B. All putative trimeric structures were predicted with the SWISS-MODEL server, after signal peptide cleavage (amino acids 1 to 22). For all panels, key features were colored for better visualization of the identified MOMP mutations (see Fig. 2 for details): β5 and β6 strands (light blue), extracellular loop L3 (orange), calcium (green sphere), and putative calcium-ligands (blue amino acid residues). For A1/A2/A3/A7 isolates, the trimer is shown (A) viewed from the side regarding the outer membrane, and (B) viewed from the inside the cell (looking out). For A4/A5/A6 isolates, only a representative monomer is displayed with the extra Asp residue depicted in red. For case B isolates (C), representative monomers are displayed viewed from the outside the cell (looking in), with the extra Asp residue depicted in red and the amino acid substitution from Gly to Asp marked in purple.

Article Snippet: Locus tag of the C. jejuni subsp. jejuni NCTC 11168 = ATCC 700819 strain (GenBank NC_002163.1 ) was adopted to designate all gene hits identified in the subsequent analyses, whenever it was possible.

Techniques: In Silico