c casp9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc c casp9
    C Casp9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    c casp9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc c casp9
    C Casp9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    c casp9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc c casp9
    6-Gingerol induced intrinsic apoptosis pathway. (A) Fluorescein-conjugated annex-in V (annexin V-FITC) vs . propidium iodide (PI) staining analysis in NCCIT and NTERA-2 cells following incubation with 200 μM 6-gingerol for 48 h. (B) Western blot of BAX, BCL-2, BCL-xL, <t>C-Casp9,</t> Casp9, and cytochrome c in NCCIT and NTERA-2 cells with 100 or 200 μM 6-gingerol for 48 h. Expression levels were estimated by densitometry and normalized to β-actin. Data were obtained in triplicate. (C) Real-time qPCR analysis showing illustrative expression of BAX , BCL-2 , and <t>caspase</t> <t>9</t> genes in NCCIT and NTERA-2 cells incubated with 100 or 200 μM 6-gingerol for 48 h. Next, the obtained Cp values were normalized to GAPDH mRNA. Controls were set at 100. *** p < 0.001 (ANOVA test). # p < 0.001 vs . control. (D) Western blot of cyto-chrome c in cytosolic and mitochondrial fractions isolated from NCCIT and NTERA-2 cells after 48 h treatment with 200 μM of 6-gingerol. GAPDH and COXIV were the controls for cyto-solic and mitochondrial fractions, respectively. (E) A plot of the ATP concentration following treatment with 200 μM of 6-gingerol in NCCIT and NTERA-2 cells. Controls were set at 100. *** p < 0.001 (Student’s t-test).
    C Casp9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 1 article reviews
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    c casp9 - by Bioz Stars, 2023-03
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    1) Product Images from "Pivotal Role of Iron Homeostasis in the Induction of Mitochondrial Apoptosis by 6-Gingerol Through PTEN Regulated PD-L1 Expression in Embryonic Cancer Cells"

    Article Title: Pivotal Role of Iron Homeostasis in the Induction of Mitochondrial Apoptosis by 6-Gingerol Through PTEN Regulated PD-L1 Expression in Embryonic Cancer Cells

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2021.781720

    6-Gingerol induced intrinsic apoptosis pathway. (A) Fluorescein-conjugated annex-in V (annexin V-FITC) vs . propidium iodide (PI) staining analysis in NCCIT and NTERA-2 cells following incubation with 200 μM 6-gingerol for 48 h. (B) Western blot of BAX, BCL-2, BCL-xL, C-Casp9, Casp9, and cytochrome c in NCCIT and NTERA-2 cells with 100 or 200 μM 6-gingerol for 48 h. Expression levels were estimated by densitometry and normalized to β-actin. Data were obtained in triplicate. (C) Real-time qPCR analysis showing illustrative expression of BAX , BCL-2 , and caspase 9 genes in NCCIT and NTERA-2 cells incubated with 100 or 200 μM 6-gingerol for 48 h. Next, the obtained Cp values were normalized to GAPDH mRNA. Controls were set at 100. *** p < 0.001 (ANOVA test). # p < 0.001 vs . control. (D) Western blot of cyto-chrome c in cytosolic and mitochondrial fractions isolated from NCCIT and NTERA-2 cells after 48 h treatment with 200 μM of 6-gingerol. GAPDH and COXIV were the controls for cyto-solic and mitochondrial fractions, respectively. (E) A plot of the ATP concentration following treatment with 200 μM of 6-gingerol in NCCIT and NTERA-2 cells. Controls were set at 100. *** p < 0.001 (Student’s t-test).
    Figure Legend Snippet: 6-Gingerol induced intrinsic apoptosis pathway. (A) Fluorescein-conjugated annex-in V (annexin V-FITC) vs . propidium iodide (PI) staining analysis in NCCIT and NTERA-2 cells following incubation with 200 μM 6-gingerol for 48 h. (B) Western blot of BAX, BCL-2, BCL-xL, C-Casp9, Casp9, and cytochrome c in NCCIT and NTERA-2 cells with 100 or 200 μM 6-gingerol for 48 h. Expression levels were estimated by densitometry and normalized to β-actin. Data were obtained in triplicate. (C) Real-time qPCR analysis showing illustrative expression of BAX , BCL-2 , and caspase 9 genes in NCCIT and NTERA-2 cells incubated with 100 or 200 μM 6-gingerol for 48 h. Next, the obtained Cp values were normalized to GAPDH mRNA. Controls were set at 100. *** p < 0.001 (ANOVA test). # p < 0.001 vs . control. (D) Western blot of cyto-chrome c in cytosolic and mitochondrial fractions isolated from NCCIT and NTERA-2 cells after 48 h treatment with 200 μM of 6-gingerol. GAPDH and COXIV were the controls for cyto-solic and mitochondrial fractions, respectively. (E) A plot of the ATP concentration following treatment with 200 μM of 6-gingerol in NCCIT and NTERA-2 cells. Controls were set at 100. *** p < 0.001 (Student’s t-test).

    Techniques Used: Staining, Incubation, Western Blot, Expressing, Isolation, Concentration Assay

    c casp9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc c casp9
    C Casp9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc c casp9
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    c casp9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc c casp9
    C Casp9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc c casp9
    C Casp9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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