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c 12281  (PromoCell)


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    PromoCell c 12281
    C 12281, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+12281/HPMEC-c+Human+Pulmonary+Microvascular+Endothelial+Cells/pmc12947425-74-10-9
    Average 96 stars, based on 162 article reviews
    c 12281 - by Bioz Stars, 2026-09
    96/100 stars

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    other:

    Article Title: An approach to evaluate the effect of inflammatory microvesicles on Ca 2+ handling in human-induced pluripotent stem cell-derived cardiomyocytes
    Article Snippet: Primary human pulmonary microvascular endothelial cells (HPMEC) were commercially obtained from Promocell (C-12281, Heidelberg, Germany).

    Cell Culture:

    Article Title: TRPV2 channels facilitate pulmonary endothelial barrier recovery after ROS-induced permeability
    Article Snippet: .. Primary human pulmonary microvascular endothelial cells (HPMECs) [ ] from healthy donors were obtained from Promocell (Heidelberg, Germany, #C-12281) and cultured in endothelial cell growth medium MV (Promocell, #C-22020) at 37 °C and 5 % CO 2 , and were kept until passage 12. ..

    Article Title: TRPV2 channels facilitate pulmonary endothelial barrier recovery after ROS-induced permeability
    Article Snippet: .. Cells 80 Primary human pulmonary microvascular endothelial cells (HPMECs) [29] from healthy 81 donors were obtained from Promocell (Heidelberg, Germany, #C-12281) and cultured in 82 endothelial cell growth medium MV (Promocell, #C-22020) at 37 °C and 5 % CO2, and were kept 83 until passage 12. ..

    Article Title: MiR-483-5p has the potential to serve as a biomarker in perioperative respiratory adverse events in children under general anesthesia.
    Article Snippet: .. Establishment of the PRAE cell model Human pulmonary microvascular endothelial cells (HPMECs) were provided by PromoCell (C-12281, Heidelberg, Germany) and cultured in endothelial cell medium (ECM) supplemented with FBS, antibiotics, and endothelial cell growth serum. ..

    Article Title: MiR-483-5p has the potential to serve as a biomarker in perioperative respiratory adverse events in children under general anesthesia
    Article Snippet: .. Human pulmonary microvascular endothelial cells (HPMECs) were provided by PromoCell (C-12281, Heidelberg, Germany) and cultured in endothelial cell medium (ECM) supplemented with FBS, antibiotics, and endothelial cell growth serum. ..

    Article Title: TRPV2 and TRPM2 channels facilitate pulmonary endothelial barrier recovery after ROS-induced permeability
    Article Snippet: .. Primary human pulmonary microvascular endothelial cells (HPMECs) [ ] from healthy donors were obtained from Promocell (Heidelberg, Germany, #C-12281) and cultured in endothelial cell growth medium MV (Promocell, #C-22020) at 37 °C and 5 % CO 2 , and were kept until passage 12. ..

    Article Title: Oxidized Cell‐Free Hemoglobin Induces Mitochondrial Dysfunction by Activation of the Mitochondrial Permeability Transition Pore in the Pulmonary Microvasculature
    Article Snippet: .. Primary human lung microvascular endothelial cells (HLMVECs) were purchased from PromoCell (C‐12281; Heidelberg, Germany) and cultured in Endothelial Cell Growth Media MV2 (C‐22121; PromoCell). .. Cells were grown to confluence on tissue culture plates coated with Attachment Factor Protein (S006100; Gibco) in a humidified 5% CO 2 incubator at 37°C.

    Isolation:

    Article Title: sVEGFR1 Is Enriched in Hepatic Vein Blood—Evidence for a Provisional Hepatic Factor Candidate?
    Article Snippet: .. Primary human pulmonary microvascular endothelial cells (HPMECs) isolated from the lung of a single donor were purchased and used for all experiments (Promocell, #C-12281). .. Mycoplasma-free cells were grown and maintained in Endothelial cell growth medium MV (Promocell, #C-22020) and studied at passage 4–6.



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    Multiplexed ELISA of conditioned media from unstimulated umbilical artery (HUAEC), umbilical vein (HUVEC), dermal microvascular (HDMEC), and pulmonary microvascular (HPMEC) endothelial cells. Conditioned media was collected from 1 well per cell type after 16 hours incubation. Samples were analyzed with a Luminex Magpix device and a custom ProcartaPlex inflammation panel. Data are represented as (A) Heatmap normalized across the range for each protein, with darker tones corresponding to higher expression and less expression for lighter tones. Factors that measured below the limit of detection are indicated with an X. (B) log 10 (average expression) ± SEM for N=4 independent experiments. Factors that measured below the limit of detection are indicated with an X. Full statistical analysis with one-way ANOVA and Tukey-Kramer pairwise comparisons testing was done for average expression across all cell types, with results presented in Supplemental Figure 1.

    Journal: bioRxiv

    Article Title: Endothelial Heterogeneity Across Vascular Beds Impacts Inflammatory Signaling and Neutrophil Adhesion

    doi: 10.64898/2026.05.26.727909

    Figure Lengend Snippet: Multiplexed ELISA of conditioned media from unstimulated umbilical artery (HUAEC), umbilical vein (HUVEC), dermal microvascular (HDMEC), and pulmonary microvascular (HPMEC) endothelial cells. Conditioned media was collected from 1 well per cell type after 16 hours incubation. Samples were analyzed with a Luminex Magpix device and a custom ProcartaPlex inflammation panel. Data are represented as (A) Heatmap normalized across the range for each protein, with darker tones corresponding to higher expression and less expression for lighter tones. Factors that measured below the limit of detection are indicated with an X. (B) log 10 (average expression) ± SEM for N=4 independent experiments. Factors that measured below the limit of detection are indicated with an X. Full statistical analysis with one-way ANOVA and Tukey-Kramer pairwise comparisons testing was done for average expression across all cell types, with results presented in Supplemental Figure 1.

    Article Snippet: The following cells were used: pooled human umbilical vein endothelial cells (HUVEC, PromoCell C-12203, Heidelberg, Germany), human umbilical arterial endothelial cells (HUAEC, PromoCell C-12202, Heidelberg, Germany), adult dermal microvascular endothelial cells (HDMEC, PromoCell C-12212, Heidelberg, Germany), and adult pulmonary microvascular endothelial cells (HPMEC, PromoCell C-12281, Heidelberg, Germany).

    Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Luminex, Expressing

    Multiplexed ELISA of conditioned media from TNF-stimulated (50ng/mL TNF in media) umbilical artery (HUAEC), umbilical vein (HUVEC), dermal microvascular (HDMEC), and pulmonary microvascular (HPMEC) endothelial cells. Conditioned media was collected from 1 well per cell type after 16 hours incubation. Samples were analyzed with a Luminex Magpix device and a custom ProcartaPlex inflammation panel. (A) Heatmap normalized across the range for each protein, with darker tones corresponding to higher expression and less expression for lighter tones. (B) Fold-change expression differences from unstimulated control for factors with >8 fold increase by at least one cell type. Data are average fold change-expression for N=4 independent experiments. One-way ANOVA with Tukey-Kramer pairwise comparisons testing was done for each condition, with asterisks indicating statistical significance between conditions (*p<0.05).

    Journal: bioRxiv

    Article Title: Endothelial Heterogeneity Across Vascular Beds Impacts Inflammatory Signaling and Neutrophil Adhesion

    doi: 10.64898/2026.05.26.727909

    Figure Lengend Snippet: Multiplexed ELISA of conditioned media from TNF-stimulated (50ng/mL TNF in media) umbilical artery (HUAEC), umbilical vein (HUVEC), dermal microvascular (HDMEC), and pulmonary microvascular (HPMEC) endothelial cells. Conditioned media was collected from 1 well per cell type after 16 hours incubation. Samples were analyzed with a Luminex Magpix device and a custom ProcartaPlex inflammation panel. (A) Heatmap normalized across the range for each protein, with darker tones corresponding to higher expression and less expression for lighter tones. (B) Fold-change expression differences from unstimulated control for factors with >8 fold increase by at least one cell type. Data are average fold change-expression for N=4 independent experiments. One-way ANOVA with Tukey-Kramer pairwise comparisons testing was done for each condition, with asterisks indicating statistical significance between conditions (*p<0.05).

    Article Snippet: The following cells were used: pooled human umbilical vein endothelial cells (HUVEC, PromoCell C-12203, Heidelberg, Germany), human umbilical arterial endothelial cells (HUAEC, PromoCell C-12202, Heidelberg, Germany), adult dermal microvascular endothelial cells (HDMEC, PromoCell C-12212, Heidelberg, Germany), and adult pulmonary microvascular endothelial cells (HPMEC, PromoCell C-12281, Heidelberg, Germany).

    Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Luminex, Expressing, Control

    Multiplexed ELISA of conditioned media from P. aeuriginosa -stimulated (0.05 OD in media) umbilical artery (HUAEC), umbilical vein (HUVEC), dermal microvascular (HDMEC), and pulmonary microvascular (HPMEC) endothelial cells. Conditioned media was collected from 1 well per cell type after 16 hours incubation. Samples were analyzed with a Luminex Magpix device and a custom ProcartaPlex inflammation panel. (A) Heatmap normalized across the range for each protein, with darker tones corresponding to higher expression and less expression for lighter tones. (B) Fold-change expression differences from unstimulated control for factors with >8 fold increase by at least one cell type. Data are average fold change-expression for N=4 independent experiments. One-way ANOVA with Tukey-Kramer pairwise comparisons testing was done for each condition, with asterisks indicating statistical significance between conditions (*p<0.05).

    Journal: bioRxiv

    Article Title: Endothelial Heterogeneity Across Vascular Beds Impacts Inflammatory Signaling and Neutrophil Adhesion

    doi: 10.64898/2026.05.26.727909

    Figure Lengend Snippet: Multiplexed ELISA of conditioned media from P. aeuriginosa -stimulated (0.05 OD in media) umbilical artery (HUAEC), umbilical vein (HUVEC), dermal microvascular (HDMEC), and pulmonary microvascular (HPMEC) endothelial cells. Conditioned media was collected from 1 well per cell type after 16 hours incubation. Samples were analyzed with a Luminex Magpix device and a custom ProcartaPlex inflammation panel. (A) Heatmap normalized across the range for each protein, with darker tones corresponding to higher expression and less expression for lighter tones. (B) Fold-change expression differences from unstimulated control for factors with >8 fold increase by at least one cell type. Data are average fold change-expression for N=4 independent experiments. One-way ANOVA with Tukey-Kramer pairwise comparisons testing was done for each condition, with asterisks indicating statistical significance between conditions (*p<0.05).

    Article Snippet: The following cells were used: pooled human umbilical vein endothelial cells (HUVEC, PromoCell C-12203, Heidelberg, Germany), human umbilical arterial endothelial cells (HUAEC, PromoCell C-12202, Heidelberg, Germany), adult dermal microvascular endothelial cells (HDMEC, PromoCell C-12212, Heidelberg, Germany), and adult pulmonary microvascular endothelial cells (HPMEC, PromoCell C-12281, Heidelberg, Germany).

    Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Luminex, Expressing, Control