bx63 fluorescence microscope  (Olympus)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Name:
    BX63 Automated Fluorescence Microscope
    Description:
    Fully Motorized and Easy to Use Flexible and easy to use the fully motorized BX63 microscope uses the motorized nosepiece to focus onto the sample enabling the stage to be fixed for added stability The smooth silent motorized stage is driven by ultrasonic piezo technology for precise operation
    Catalog Number:
    BX63-AUTOMATED-FLUORESCENCE-MICROSCOPE
    Price:
    None
    Category:
    Products Upright Microscopes Fluorescence Microscopes BX63
    Buy from Supplier


    Structured Review

    Olympus bx63 fluorescence microscope
    BX63 Automated Fluorescence Microscope
    Fully Motorized and Easy to Use Flexible and easy to use the fully motorized BX63 microscope uses the motorized nosepiece to focus onto the sample enabling the stage to be fixed for added stability The smooth silent motorized stage is driven by ultrasonic piezo technology for precise operation
    https://www.bioz.com/result/bx63 fluorescence microscope/product/Olympus
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bx63 fluorescence microscope - by Bioz Stars, 2021-07
    97/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Isolation of Genetically Tractable Most-Wanted Bacteria by Metaparental Mating
    Article Snippet: Vectors with putative deletions were further characterized by restriction digest analysis using the 8 bp cutting restriction enzymes flanking the individual vector modules. .. Transconjugants carrying pEHR512112 were examined using an Olympus BX 63 microscope fitted with a DP80 camera, Xcite LED light source and fluorescence filter cube U-FBN (excitation 470–495 nm, emission 510 nm). .. Images were captured using the Olympus cellSens modular imaging software platform and processed using the ImageJ software package ( http://imagej.nih.gov/ij/ ).

    Article Title: Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy
    Article Snippet: .. Each part of the experiment was imaged with Olympus BX63 microscope equipped with the PPM device, DP80 camera and LUCPlanFLN 40 × objective. .. Enzymatic digestion RNase and DNase enzymes were used from Invitrogen by Thermo Fisher Scientific RecoverAll Kit which is used for RNA purification.

    Article Title: Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy
    Article Snippet: We therefore explored this phenomenon using the technique of polychromatic polarization microscopy (PPM), which we found to provide strong and distinct signals for IBI. .. Microscope set up Polychromatic polarization micrographs of IBI were acquired using an Olympus BX63 microscope equipped with a DP80 camera and a custom spectral polarization state generator and analyzer (Supplementary Fig. ). ..

    Article Title: MIAQuant, a novel system for automatic segmentation, measurement, and localization comparison of different biomarkers from serialized histological slices
    Article Snippet: After incubation with primary antibodies (Abs) we used as relevation system REAL Detection System, Alkaline Phosphatase /RED (red color) or UltraVision™ Quanto Detection System HRP (brown color) according to the manufacturer’s instructions. .. Images were acquired by Aperio Scanscope Cs (Aperio Technologies, Vista, CA, USA, color CCD camera, 14 μm x 14 μm pixel size), Olympus BX63 equipped with DP80 camera (color CCD camera, 6.45 μm x 6.45 μm pixel size) and software cellSens (Shinjuku Monolith, Tokyo, Japan) or by Nikon Eclipse E600 microscope equipped with DS-Fi1 camera (color CCD camera, 3.4 μm x 3.4 μm) and software Nis- Elements AR3.10 (Tochigi Nikon Corporation, Tochigi, Japan). .. These imaging systems have been used to acquire images whose pixel size ranges from 5000x5000 pixels to 35000x35000, and whose resolution is in the range.

    Article Title: Buffered EGFR signaling regulated by spitz to argos expression ratio is critical for patterning the Drosophila eye
    Article Snippet: The secondary antibody was diluted to 1:1000 in nuclease-free blocking solution and incubated with tissues for 3 hours at room temperature on a rotating mixer. .. Image acquisitionThe tissues were imaged on an Olympus BX63 upright widefield fluorescence microscope with a Retiga 6000 (Qimaging) CCD monochrome camera. ..

    Article Title: 2,4-Diaminothieno[3,2-d]pyrimidines, a new class of anthelmintic with activity against adult and egg stages of whipworm
    Article Snippet: Embryonation assay One hundred unembryonated eggs were treated with water, 1% v/v DMSO in water or test compounds at a final concentration of 100 μM (unless stated) with 1% v/v DMSO, in the dark at 26°C, either for 56 days or for shorter periods as described. .. Images were collected on an Olympus BX63 upright microscope using a 60x / 1.42 PlanApo N (Oil) objective and captured and white-balanced using an DP80 camera (Olympus) in monochrome mode through CellSens Dimension v1.16 (Olympus). ..

    Article Title: Evolution of Estrogen Receptor Status from Primary Tumors to Metastasis and Serially Collected Circulating Tumor Cells
    Article Snippet: .. The cells were scanned using a BX63 Upright Microscope (Olympus Corporation, LRI, Lund, Sweden) and CellSense Dimension software. ..

    Article Title: 2,4-Diaminothieno[3,2-d]pyrimidines, a new class of anthelmintic with activity against adult and egg stages of whipworm
    Article Snippet: .. Unembryonated eggs were soaked in 100 μM OX03147 at 26°C for the duration specified and then embryonation determined and eggs imaged using an Olympus BX63 microscope. ..

    Fluorescence:

    Article Title: Isolation of Genetically Tractable Most-Wanted Bacteria by Metaparental Mating
    Article Snippet: Vectors with putative deletions were further characterized by restriction digest analysis using the 8 bp cutting restriction enzymes flanking the individual vector modules. .. Transconjugants carrying pEHR512112 were examined using an Olympus BX 63 microscope fitted with a DP80 camera, Xcite LED light source and fluorescence filter cube U-FBN (excitation 470–495 nm, emission 510 nm). .. Images were captured using the Olympus cellSens modular imaging software platform and processed using the ImageJ software package ( http://imagej.nih.gov/ij/ ).

    Article Title: Buffered EGFR signaling regulated by spitz to argos expression ratio is critical for patterning the Drosophila eye
    Article Snippet: The secondary antibody was diluted to 1:1000 in nuclease-free blocking solution and incubated with tissues for 3 hours at room temperature on a rotating mixer. .. Image acquisitionThe tissues were imaged on an Olympus BX63 upright widefield fluorescence microscope with a Retiga 6000 (Qimaging) CCD monochrome camera. ..

    Software:

    Article Title: MIAQuant, a novel system for automatic segmentation, measurement, and localization comparison of different biomarkers from serialized histological slices
    Article Snippet: After incubation with primary antibodies (Abs) we used as relevation system REAL Detection System, Alkaline Phosphatase /RED (red color) or UltraVision™ Quanto Detection System HRP (brown color) according to the manufacturer’s instructions. .. Images were acquired by Aperio Scanscope Cs (Aperio Technologies, Vista, CA, USA, color CCD camera, 14 μm x 14 μm pixel size), Olympus BX63 equipped with DP80 camera (color CCD camera, 6.45 μm x 6.45 μm pixel size) and software cellSens (Shinjuku Monolith, Tokyo, Japan) or by Nikon Eclipse E600 microscope equipped with DS-Fi1 camera (color CCD camera, 3.4 μm x 3.4 μm) and software Nis- Elements AR3.10 (Tochigi Nikon Corporation, Tochigi, Japan). .. These imaging systems have been used to acquire images whose pixel size ranges from 5000x5000 pixels to 35000x35000, and whose resolution is in the range.

    Article Title: Evolution of Estrogen Receptor Status from Primary Tumors to Metastasis and Serially Collected Circulating Tumor Cells
    Article Snippet: .. The cells were scanned using a BX63 Upright Microscope (Olympus Corporation, LRI, Lund, Sweden) and CellSense Dimension software. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Olympus bx 63 microscope
    Analysis of transconjugants carrying pEHR512112 by fluorescence microscopy. The transconjugants were recovered and purified on M2SC based medium. Colonies were re-suspended in sterile anaerobic diluent and individual cells were visualised using an Olympus <t>BX</t> 63 microscope. Images were captured using the Olympus cellSens modular imaging software platform and processed using the ImageJ software package. A scale bar of 10 μm is included for reference.
    Bx 63 Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bx 63 microscope/product/Olympus
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bx 63 microscope - by Bioz Stars, 2021-07
    97/100 stars
      Buy from Supplier

    Image Search Results


    Analysis of transconjugants carrying pEHR512112 by fluorescence microscopy. The transconjugants were recovered and purified on M2SC based medium. Colonies were re-suspended in sterile anaerobic diluent and individual cells were visualised using an Olympus BX 63 microscope. Images were captured using the Olympus cellSens modular imaging software platform and processed using the ImageJ software package. A scale bar of 10 μm is included for reference.

    Journal: Scientific Reports

    Article Title: Isolation of Genetically Tractable Most-Wanted Bacteria by Metaparental Mating

    doi: 10.1038/srep13282

    Figure Lengend Snippet: Analysis of transconjugants carrying pEHR512112 by fluorescence microscopy. The transconjugants were recovered and purified on M2SC based medium. Colonies were re-suspended in sterile anaerobic diluent and individual cells were visualised using an Olympus BX 63 microscope. Images were captured using the Olympus cellSens modular imaging software platform and processed using the ImageJ software package. A scale bar of 10 μm is included for reference.

    Article Snippet: Transconjugants carrying pEHR512112 were examined using an Olympus BX 63 microscope fitted with a DP80 camera, Xcite LED light source and fluorescence filter cube U-FBN (excitation 470–495 nm, emission 510 nm).

    Techniques: Fluorescence, Microscopy, Purification, Imaging, Software

    Unembryonated T . muris eggs treated with diaminothienopyrimidines have altered embryonation. Unembyronated eggs were soaked in 100 μM compound (unless specified otherwise) at 26°C (unless specified otherwise) for the duration of the embryonation process (56–60 days) and then embryonation determined and eggs imaged using an Olympus BX63 microscope. Scale bar indicates 10 μm. (a) Typical embryonated egg and (b) unembryonated egg. (c) treatment with DATPs increased the incidence of unembryonated eggs. Representative pictures of (d) DMSO, (e) OX02925 , (f) OX02926 , (g) OX03143 and (h) OX03147 100 μM and (i) OX03147 1 μM soaked T . muris eggs. (j) Unembyronated eggs soaked in OX03147 at room temperature for 56 days and embryonation determined. (k) Unembyronated eggs soaked in OX03147 at 26°C for 56 days and larval length calculated using ImageJ.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: 2,4-Diaminothieno[3,2-d]pyrimidines, a new class of anthelmintic with activity against adult and egg stages of whipworm

    doi: 10.1371/journal.pntd.0006487

    Figure Lengend Snippet: Unembryonated T . muris eggs treated with diaminothienopyrimidines have altered embryonation. Unembyronated eggs were soaked in 100 μM compound (unless specified otherwise) at 26°C (unless specified otherwise) for the duration of the embryonation process (56–60 days) and then embryonation determined and eggs imaged using an Olympus BX63 microscope. Scale bar indicates 10 μm. (a) Typical embryonated egg and (b) unembryonated egg. (c) treatment with DATPs increased the incidence of unembryonated eggs. Representative pictures of (d) DMSO, (e) OX02925 , (f) OX02926 , (g) OX03143 and (h) OX03147 100 μM and (i) OX03147 1 μM soaked T . muris eggs. (j) Unembyronated eggs soaked in OX03147 at room temperature for 56 days and embryonation determined. (k) Unembyronated eggs soaked in OX03147 at 26°C for 56 days and larval length calculated using ImageJ.

    Article Snippet: Images were collected on an Olympus BX63 upright microscope using a 60x / 1.42 PlanApo N (Oil) objective and captured and white-balanced using an DP80 camera (Olympus) in monochrome mode through CellSens Dimension v1.16 (Olympus).

    Techniques: Microscopy

    Segmentations computed by MIAQuant on images with differing characteristics. Sample images in the left column (A,C,E,G,I) and segmentation results in the right column (B,D,F,H,J). MIAQuant represents the segmented markers both with binary images (B and D) and with their original RGB color (F, H, J); this choice allows to highlight the specific hue characterizing each of the different marker areas. The shown sample images were acquired with different instruments: Aperio Scanscope Cs (A), Olympus BX63 equipped with DP89 camera and software cellSens (C,E), and Nikon Eclipse E600 microscope equipped with DS-Fi1 camera and software Nis-Elements AR3.10 (G-I). A,B) Immunostaining of atherosclerosis plaque with podoplanin (D2-40 Abs) stained with alkaline phosphatase in red. C-F) B-cell lymphoma xenograft model stained with Ki67 Abs stained with peroxidase in brown. E) The image is acquired with a much higher magnification than that of C. G,H) Histochemical staining with Alcian blue of normal colon tissue. I,J) Histochemical staining with Oil Red of cultured cells.

    Journal: European Journal of Histochemistry : EJH

    Article Title: MIAQuant, a novel system for automatic segmentation, measurement, and localization comparison of different biomarkers from serialized histological slices

    doi: 10.4081/ejh.2017.2838

    Figure Lengend Snippet: Segmentations computed by MIAQuant on images with differing characteristics. Sample images in the left column (A,C,E,G,I) and segmentation results in the right column (B,D,F,H,J). MIAQuant represents the segmented markers both with binary images (B and D) and with their original RGB color (F, H, J); this choice allows to highlight the specific hue characterizing each of the different marker areas. The shown sample images were acquired with different instruments: Aperio Scanscope Cs (A), Olympus BX63 equipped with DP89 camera and software cellSens (C,E), and Nikon Eclipse E600 microscope equipped with DS-Fi1 camera and software Nis-Elements AR3.10 (G-I). A,B) Immunostaining of atherosclerosis plaque with podoplanin (D2-40 Abs) stained with alkaline phosphatase in red. C-F) B-cell lymphoma xenograft model stained with Ki67 Abs stained with peroxidase in brown. E) The image is acquired with a much higher magnification than that of C. G,H) Histochemical staining with Alcian blue of normal colon tissue. I,J) Histochemical staining with Oil Red of cultured cells.

    Article Snippet: Images were acquired by Aperio Scanscope Cs (Aperio Technologies, Vista, CA, USA, color CCD camera, 14 μm x 14 μm pixel size), Olympus BX63 equipped with DP80 camera (color CCD camera, 6.45 μm x 6.45 μm pixel size) and software cellSens (Shinjuku Monolith, Tokyo, Japan) or by Nikon Eclipse E600 microscope equipped with DS-Fi1 camera (color CCD camera, 3.4 μm x 3.4 μm) and software Nis- Elements AR3.10 (Tochigi Nikon Corporation, Tochigi, Japan).

    Techniques: Marker, Software, Microscopy, Immunostaining, Staining, Cell Culture

    spitz -to- argos ratio is important for proper ommatidial patterning. (A) smFISH was performed in eye imaginal discs from represented crosses for spitz and argos mRNA in the two rows. In the third row, adult eye phenotypes are shown. The Elav > UAS spitz dsRNA progeny shows perfectly arranged ommatidia like the wildtype CantonS, while the other two crosses show rough eye phenotypes. Scale bar for widefield fluorescence images is 10μm and SEM images is 100μm. (B) and (C) mRNA numbers for spitz and argos were counted in photoreceptors and non-photoreceptors in respective crosses. spitz and argos mRNA show variation in number as expected from the respective crosses. (D) spitz -to- argos mRNA ratio in eye field irrespective of cell type (PR or non-PR) was calculated as dosage was known to be important for ommatidial pattern formation (N=9 tissues in all the crosses). Most remarkably, knocking down spitz in PR cells with a Elav driver, knocks down argos numbers too, and overall the ratio is unchanged. When a GMR driver is used to knockdown either argos or spitz in the full field, the ratio is higher or lower than wildtype. Error bars in (B), (C) and (D) are standard errors of mean.

    Journal: bioRxiv

    Article Title: Buffered EGFR signaling regulated by spitz to argos expression ratio is critical for patterning the Drosophila eye

    doi: 10.1101/2021.05.19.444784

    Figure Lengend Snippet: spitz -to- argos ratio is important for proper ommatidial patterning. (A) smFISH was performed in eye imaginal discs from represented crosses for spitz and argos mRNA in the two rows. In the third row, adult eye phenotypes are shown. The Elav > UAS spitz dsRNA progeny shows perfectly arranged ommatidia like the wildtype CantonS, while the other two crosses show rough eye phenotypes. Scale bar for widefield fluorescence images is 10μm and SEM images is 100μm. (B) and (C) mRNA numbers for spitz and argos were counted in photoreceptors and non-photoreceptors in respective crosses. spitz and argos mRNA show variation in number as expected from the respective crosses. (D) spitz -to- argos mRNA ratio in eye field irrespective of cell type (PR or non-PR) was calculated as dosage was known to be important for ommatidial pattern formation (N=9 tissues in all the crosses). Most remarkably, knocking down spitz in PR cells with a Elav driver, knocks down argos numbers too, and overall the ratio is unchanged. When a GMR driver is used to knockdown either argos or spitz in the full field, the ratio is higher or lower than wildtype. Error bars in (B), (C) and (D) are standard errors of mean.

    Article Snippet: Image acquisitionThe tissues were imaged on an Olympus BX63 upright widefield fluorescence microscope with a Retiga 6000 (Qimaging) CCD monochrome camera.

    Techniques: Fluorescence

    Overexpression of EGFR completely disrupts ommatidial pattern from the adult eye. (A) Eye imaginal discs of GMR > UAS EGFR CA /TM6 progeny stained for spitz and argos mRNA. From the same cross, EGFR CA and non-EGFR CA larvae were distinguished using the tubby phenotype. Scale bar for widefield images is 10μm. Exclusive expression of spitz and argos is lost in EGFR CA which leads to change in the spitz -to- argos ratio represented in (B) [CantonS N=9, EGFR CA N=10, non-EGFR CA N=9]. (C) 170X SEM images of GMR > UAS EGFR CA /TM6 progeny. The ommatidial pattern is lost when EGFR is constitutively active and the size of the eye is also reduced. Heatshock was administered for 6 hrs to EGFR CA and non-EGFR CA larvae for maximal activation of the cassette. Scale bar is 10μm. (D) 170X SEM images of GMR > UAS EGFR CA adult flies corresponding to different time points of heat shock at 29°C. N=8 for all time points. (E) spitz -to- argos ratios in eye discs corresponding to different time points of heat shock at 29°C. Quantification of absolute mRNA numbers and ratios represented in all the above plots were calculated in the eye field irrespective of the cell type. Error bars in all the plots are standard errors of mean. (F) Adult eyes corresponding to different time points of heat shock at 29°C. Scale bar is 100μm. No rough-eye phenotype is seen for 20 min of heatshock, though a difference in the spitz -to- argos ratio is already seen at this point. Beyond this the eyes are fully rough, and the phenotype is fully penetrant. This is indicative of developmental buffering.

    Journal: bioRxiv

    Article Title: Buffered EGFR signaling regulated by spitz to argos expression ratio is critical for patterning the Drosophila eye

    doi: 10.1101/2021.05.19.444784

    Figure Lengend Snippet: Overexpression of EGFR completely disrupts ommatidial pattern from the adult eye. (A) Eye imaginal discs of GMR > UAS EGFR CA /TM6 progeny stained for spitz and argos mRNA. From the same cross, EGFR CA and non-EGFR CA larvae were distinguished using the tubby phenotype. Scale bar for widefield images is 10μm. Exclusive expression of spitz and argos is lost in EGFR CA which leads to change in the spitz -to- argos ratio represented in (B) [CantonS N=9, EGFR CA N=10, non-EGFR CA N=9]. (C) 170X SEM images of GMR > UAS EGFR CA /TM6 progeny. The ommatidial pattern is lost when EGFR is constitutively active and the size of the eye is also reduced. Heatshock was administered for 6 hrs to EGFR CA and non-EGFR CA larvae for maximal activation of the cassette. Scale bar is 10μm. (D) 170X SEM images of GMR > UAS EGFR CA adult flies corresponding to different time points of heat shock at 29°C. N=8 for all time points. (E) spitz -to- argos ratios in eye discs corresponding to different time points of heat shock at 29°C. Quantification of absolute mRNA numbers and ratios represented in all the above plots were calculated in the eye field irrespective of the cell type. Error bars in all the plots are standard errors of mean. (F) Adult eyes corresponding to different time points of heat shock at 29°C. Scale bar is 100μm. No rough-eye phenotype is seen for 20 min of heatshock, though a difference in the spitz -to- argos ratio is already seen at this point. Beyond this the eyes are fully rough, and the phenotype is fully penetrant. This is indicative of developmental buffering.

    Article Snippet: Image acquisitionThe tissues were imaged on an Olympus BX63 upright widefield fluorescence microscope with a Retiga 6000 (Qimaging) CCD monochrome camera.

    Techniques: Over Expression, Staining, Expressing, Activation Assay