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Olympus bx61 light microscope
Relocation of Rap1p ( a ) and Sir3p ( b ) from telomeres to the nucleolus and changes in expression patterns of Rap1p, Sir3p and Nop2p ( c ) during chronological aging. a, b After 2, 7 and 14 days, Rap1p ( a ) and Sir3p ( b ) immunostaining was performed ( green ). DNA was visualised using DAPI staining ( blue ). Immunostained cells were captured with an Olympus <t>BX61</t> light microscope equipped with a DP72 CCD camera and Olympus CellF software. Nop2p was used as a nucleolar marker ( red ). Typical micrographs of haploid wild type BY4741 are shown. c After 2 and 7 days, changes in the expression of Rap1p, Sir3p and Nop2p were revealed with western blotting. For the loading control, an antibody against actin was used. The chemiluminescence signal was detected using an ECL Plus Western Blotting Detection System (GE Healthcare) and G:BOX imaging system (Syngene). Top haploid strains, bottom diploid strains. (Color figure online)
Bx61 Light Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 91/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bx61 light microscope/product/Olympus
Average 91 stars, based on 102 article reviews
Price from $9.99 to $1999.99
bx61 light microscope - by Bioz Stars, 2020-08
91/100 stars

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1) Product Images from "Links between nucleolar activity, rDNA stability, aneuploidy and chronological aging in the yeast Saccharomyces cerevisiae"

Article Title: Links between nucleolar activity, rDNA stability, aneuploidy and chronological aging in the yeast Saccharomyces cerevisiae

Journal: Biogerontology

doi: 10.1007/s10522-014-9499-y

Relocation of Rap1p ( a ) and Sir3p ( b ) from telomeres to the nucleolus and changes in expression patterns of Rap1p, Sir3p and Nop2p ( c ) during chronological aging. a, b After 2, 7 and 14 days, Rap1p ( a ) and Sir3p ( b ) immunostaining was performed ( green ). DNA was visualised using DAPI staining ( blue ). Immunostained cells were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. Nop2p was used as a nucleolar marker ( red ). Typical micrographs of haploid wild type BY4741 are shown. c After 2 and 7 days, changes in the expression of Rap1p, Sir3p and Nop2p were revealed with western blotting. For the loading control, an antibody against actin was used. The chemiluminescence signal was detected using an ECL Plus Western Blotting Detection System (GE Healthcare) and G:BOX imaging system (Syngene). Top haploid strains, bottom diploid strains. (Color figure online)
Figure Legend Snippet: Relocation of Rap1p ( a ) and Sir3p ( b ) from telomeres to the nucleolus and changes in expression patterns of Rap1p, Sir3p and Nop2p ( c ) during chronological aging. a, b After 2, 7 and 14 days, Rap1p ( a ) and Sir3p ( b ) immunostaining was performed ( green ). DNA was visualised using DAPI staining ( blue ). Immunostained cells were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. Nop2p was used as a nucleolar marker ( red ). Typical micrographs of haploid wild type BY4741 are shown. c After 2 and 7 days, changes in the expression of Rap1p, Sir3p and Nop2p were revealed with western blotting. For the loading control, an antibody against actin was used. The chemiluminescence signal was detected using an ECL Plus Western Blotting Detection System (GE Healthcare) and G:BOX imaging system (Syngene). Top haploid strains, bottom diploid strains. (Color figure online)

Techniques Used: Expressing, Immunostaining, Staining, Light Microscopy, Software, Marker, Western Blot, Imaging

CA-induced aneuploidy. Aneuploidy was assessed using fluorescence in situ hybridisation (FISH) using whole chromosome painting probes (WCPPs). After 2 and 28 days, cells were taken from chronological aging culture and transfer to fresh YPD medium to continue mitotic growth. A biotinylated probe specific to chromosome I or chromosome V (Polish Patent Office, registration number P.404526) was added to the spheroplast-coated slide and its detection was performed with a Star*FISH © Biotin Painting Kit—FITC Label (Cambio). Fluorescent signals were analysed using an Olympus BX61 fluorescence microscope equipped with a DP72 CCD camera and Olympus CellF software. a The frequencies of aneuploidy events (chromosome I or chromosome V): haploid strains ( left ), diploid strains ( right ). Aneuploidy events were counted and presented as a percentage of 200 total cell scores. b Typical micrographs showing chromosome I or chromosome V fluorescent signals are presented ( green ). DNA was visualised using DAPI staining ( blue ). (Color figure online)
Figure Legend Snippet: CA-induced aneuploidy. Aneuploidy was assessed using fluorescence in situ hybridisation (FISH) using whole chromosome painting probes (WCPPs). After 2 and 28 days, cells were taken from chronological aging culture and transfer to fresh YPD medium to continue mitotic growth. A biotinylated probe specific to chromosome I or chromosome V (Polish Patent Office, registration number P.404526) was added to the spheroplast-coated slide and its detection was performed with a Star*FISH © Biotin Painting Kit—FITC Label (Cambio). Fluorescent signals were analysed using an Olympus BX61 fluorescence microscope equipped with a DP72 CCD camera and Olympus CellF software. a The frequencies of aneuploidy events (chromosome I or chromosome V): haploid strains ( left ), diploid strains ( right ). Aneuploidy events were counted and presented as a percentage of 200 total cell scores. b Typical micrographs showing chromosome I or chromosome V fluorescent signals are presented ( green ). DNA was visualised using DAPI staining ( blue ). (Color figure online)

Techniques Used: Fluorescence, In Situ, Hybridization, Fluorescence In Situ Hybridization, Microscopy, Software, Staining

DNA breaks and structural aberrations within chromosome XII during chronological aging. After 2 and 14 days, yeast chromosomes were separated with PFGE and selected bands were cut with a razor blade. DNA breaks and structural aberrations within chromosome XII were visualised via alkaline chromosome comet assay (pH > 13). After electrophoresis, chromosomal DNA was stained with YOYO-1 (Molecular Probes) stain solution ( green ). a DNA breaks ( white arrowheads ) and structural aberrations (here termed as abnormal chromosome morphology) ( pink arrowheads ) were visualised using an Olympus BX61 fluorescence microscope equipped with a DP72 CCD camera and Olympus CellF software. Left haploid strains; right diploid strains; bottom positive control for DNA breaks, haploid wild type treated with 1 mM hydrogen peroxide for 10 min. b, c A total of 100 chromosomes per sample triplicate were analysed and the percentages of DNA damage and structural aberrations were calculated. b Haploid strains, c diploid strains. (Color figure online)
Figure Legend Snippet: DNA breaks and structural aberrations within chromosome XII during chronological aging. After 2 and 14 days, yeast chromosomes were separated with PFGE and selected bands were cut with a razor blade. DNA breaks and structural aberrations within chromosome XII were visualised via alkaline chromosome comet assay (pH > 13). After electrophoresis, chromosomal DNA was stained with YOYO-1 (Molecular Probes) stain solution ( green ). a DNA breaks ( white arrowheads ) and structural aberrations (here termed as abnormal chromosome morphology) ( pink arrowheads ) were visualised using an Olympus BX61 fluorescence microscope equipped with a DP72 CCD camera and Olympus CellF software. Left haploid strains; right diploid strains; bottom positive control for DNA breaks, haploid wild type treated with 1 mM hydrogen peroxide for 10 min. b, c A total of 100 chromosomes per sample triplicate were analysed and the percentages of DNA damage and structural aberrations were calculated. b Haploid strains, c diploid strains. (Color figure online)

Techniques Used: Single Cell Gel Electrophoresis, Electrophoresis, Staining, Fluorescence, Microscopy, Software, Positive Control

CA-mediated changes in nucleolus size and nucleolus/nucleus ratio. To visualise nucleoli, silver staining of nucleolar organiser regions (AgNOR) was performed and nucleoli images were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. The nucleolus size and nucleolus/nucleus ratio were calculated using ImageJ software http://rsbweb.nih.gov/ij/ . a Nucleolus size of chronologically aging haploid strains ( left ) and diploid strains ( right ). b Nucleolus/nucleus ratio of chronologically aging haploid strains ( left ) and diploid strains ( right )
Figure Legend Snippet: CA-mediated changes in nucleolus size and nucleolus/nucleus ratio. To visualise nucleoli, silver staining of nucleolar organiser regions (AgNOR) was performed and nucleoli images were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. The nucleolus size and nucleolus/nucleus ratio were calculated using ImageJ software http://rsbweb.nih.gov/ij/ . a Nucleolus size of chronologically aging haploid strains ( left ) and diploid strains ( right ). b Nucleolus/nucleus ratio of chronologically aging haploid strains ( left ) and diploid strains ( right )

Techniques Used: Silver Staining, Light Microscopy, Software

The nucleolus is fragmented during chronological aging. To visualise nucleoli, silver staining of nucleolar organiser regions (AgNOR) was performed and nucleoli images were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. a A scheme showing CA-mediated changes in nucleolar morphology. A general tendency is presented (both schematic and microscopic representations of the haploid and diploid wild type strain nucleoli are shown). b Graphs showing the percentage of normal ( black ), increased ( light grey ) and fragmented ( dark grey ) nucleoli in chronologically aging haploid strains ( left ) and diploid strains ( right ). A total of 100 cells were analysed, and their nucleolus morphological type was determined [%]. The results represent the mean from at least three independent experiments
Figure Legend Snippet: The nucleolus is fragmented during chronological aging. To visualise nucleoli, silver staining of nucleolar organiser regions (AgNOR) was performed and nucleoli images were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. a A scheme showing CA-mediated changes in nucleolar morphology. A general tendency is presented (both schematic and microscopic representations of the haploid and diploid wild type strain nucleoli are shown). b Graphs showing the percentage of normal ( black ), increased ( light grey ) and fragmented ( dark grey ) nucleoli in chronologically aging haploid strains ( left ) and diploid strains ( right ). A total of 100 cells were analysed, and their nucleolus morphological type was determined [%]. The results represent the mean from at least three independent experiments

Techniques Used: Silver Staining, Light Microscopy, Software

Related Articles

Microscopy:

Article Title: Posterior thalamic nucleus axon terminals have different structure and functional impact in the motor and somatosensory vibrissal cortices
Article Snippet: .. Stereological axon measurements Stereological analyses were carried out with a BX61 light microscope (Olympus, Japan) equipped with a MT12 microcator (0.5-µm resolution; Heidenhain, Traunreut, Germany), a X–Y–Z high-precision motorized microscope stage (ProScan II; Prior Scientific, Cambridge, UK), and an Olympus DP-71 digital camera connected to a computer running the NewCAST stereology software package (Visiopharm, Hørsholm, Denmark). .. In samples from three different Po axon-labeling experiments, absolute axonal length in individual cortical layers of S1BF and M1wk was estimated using the isotropic virtual plane method following a fractionator sampling scheme (Drøjdahl et al. ).

Light Microscopy:

Article Title: Links between nucleolar activity, rDNA stability, aneuploidy and chronological aging in the yeast Saccharomyces cerevisiae
Article Snippet: .. Silver staining of nucleolar argyrophilic proteins was conducted according to Howell and Black ( ) by incubating the cell slides with a colloidal developer containing 50 % AgNO3 in the dark at 37 °C for 15 min. After washing in tap water, the preparations were stained with 5 % Giemsa for 10 s and nucleoli were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. ..

Article Title: Identifying the Role of Complement in Triggering Neuroinflammation after Traumatic Brain Injury
Article Snippet: .. Epifluorescence microscopy was used to image full brain sections using an Olympus BX61 light microscope with Visiopharm image acquisition and analysis software. .. Intensity-based analysis was automatically computed by using MATLAB (MathWorks) by normalizing the intensity of signal to the area of tissue displayed per random filed.

Article Title: Identification of p18INK4c as a Tumor Suppressor Gene in Glioblastoma Multiforme
Article Snippet: .. All imaging was performed on an Olympus BX61 light microscope with a ×40 Plan-Apochromat objective. .. In an effort to identify novel copy number alterations that drive the pathogenesis of GBM, we initially interrogated genomic DNA derived from 35 GBM cell lines and xenografts with Affymetrix SNP microarrays, as described in Materials and Methods.

Article Title: Posterior thalamic nucleus axon terminals have different structure and functional impact in the motor and somatosensory vibrissal cortices
Article Snippet: .. Stereological axon measurements Stereological analyses were carried out with a BX61 light microscope (Olympus, Japan) equipped with a MT12 microcator (0.5-µm resolution; Heidenhain, Traunreut, Germany), a X–Y–Z high-precision motorized microscope stage (ProScan II; Prior Scientific, Cambridge, UK), and an Olympus DP-71 digital camera connected to a computer running the NewCAST stereology software package (Visiopharm, Hørsholm, Denmark). .. In samples from three different Po axon-labeling experiments, absolute axonal length in individual cortical layers of S1BF and M1wk was estimated using the isotropic virtual plane method following a fractionator sampling scheme (Drøjdahl et al. ).

Article Title: A Comparative Study of the Arabidopsis thaliana Guard-Cell Transcriptome and Its Modulation by Sucrose
Article Snippet: .. Clear nail polish was painted on to the leaf impressions, peeled off when dry, and observed at 200X magnification using an Olympus BX61 light microscope. .. All stomata in the microscope’s camera field of view were counted.

Article Title: A comparative analysis of ESM-1 and vascular endothelial cell marker (CD34/CD105) expression on pituitary adenoma invasion
Article Snippet: .. To determine the MVD, tissue sections stained with CD105 and CD34 were examined using an Olympus BX61 light microscope. ..

Article Title: Light reintroduction after dark exposure reactivates plasticity in adults via perisynaptic activation of MMP-9
Article Snippet: .. “Neurolucida (MBF Bioscience, St. Albans, VT) with an Olympus BX61 light microscope was used to trace dendritic morphologies. .. Using a 40X lens (Olympus Plan N, NA=0.65), dendritic arbors of layer 4 neurons were traced, followed by sholl analysis in Neuroexplorer (MBF Bioscience) to identify the region 75 to 100 μm from soma of basolateral dendrite, which were then traced using a 100X lens (Olympus Plan N Oil, NA=1.25).

Article Title: Targeting Extracellular Cyclophilin A Reduces Neuroinflammation and Extends Survival in a Mouse Model of Amyotrophic Lateral Sclerosis
Article Snippet: .. Sections were examined under an Olympus BX61 light microscope. ..

Imaging:

Article Title: Identification of p18INK4c as a Tumor Suppressor Gene in Glioblastoma Multiforme
Article Snippet: .. All imaging was performed on an Olympus BX61 light microscope with a ×40 Plan-Apochromat objective. .. In an effort to identify novel copy number alterations that drive the pathogenesis of GBM, we initially interrogated genomic DNA derived from 35 GBM cell lines and xenografts with Affymetrix SNP microarrays, as described in Materials and Methods.

Staining:

Article Title: Links between nucleolar activity, rDNA stability, aneuploidy and chronological aging in the yeast Saccharomyces cerevisiae
Article Snippet: .. Silver staining of nucleolar argyrophilic proteins was conducted according to Howell and Black ( ) by incubating the cell slides with a colloidal developer containing 50 % AgNO3 in the dark at 37 °C for 15 min. After washing in tap water, the preparations were stained with 5 % Giemsa for 10 s and nucleoli were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. ..

Article Title: A comparative analysis of ESM-1 and vascular endothelial cell marker (CD34/CD105) expression on pituitary adenoma invasion
Article Snippet: .. To determine the MVD, tissue sections stained with CD105 and CD34 were examined using an Olympus BX61 light microscope. ..

Silver Staining:

Article Title: Links between nucleolar activity, rDNA stability, aneuploidy and chronological aging in the yeast Saccharomyces cerevisiae
Article Snippet: .. Silver staining of nucleolar argyrophilic proteins was conducted according to Howell and Black ( ) by incubating the cell slides with a colloidal developer containing 50 % AgNO3 in the dark at 37 °C for 15 min. After washing in tap water, the preparations were stained with 5 % Giemsa for 10 s and nucleoli were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. ..

Epifluorescence Microscopy:

Article Title: Identifying the Role of Complement in Triggering Neuroinflammation after Traumatic Brain Injury
Article Snippet: .. Epifluorescence microscopy was used to image full brain sections using an Olympus BX61 light microscope with Visiopharm image acquisition and analysis software. .. Intensity-based analysis was automatically computed by using MATLAB (MathWorks) by normalizing the intensity of signal to the area of tissue displayed per random filed.

Software:

Article Title: Links between nucleolar activity, rDNA stability, aneuploidy and chronological aging in the yeast Saccharomyces cerevisiae
Article Snippet: .. Silver staining of nucleolar argyrophilic proteins was conducted according to Howell and Black ( ) by incubating the cell slides with a colloidal developer containing 50 % AgNO3 in the dark at 37 °C for 15 min. After washing in tap water, the preparations were stained with 5 % Giemsa for 10 s and nucleoli were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. ..

Article Title: Identifying the Role of Complement in Triggering Neuroinflammation after Traumatic Brain Injury
Article Snippet: .. Epifluorescence microscopy was used to image full brain sections using an Olympus BX61 light microscope with Visiopharm image acquisition and analysis software. .. Intensity-based analysis was automatically computed by using MATLAB (MathWorks) by normalizing the intensity of signal to the area of tissue displayed per random filed.

Article Title: Posterior thalamic nucleus axon terminals have different structure and functional impact in the motor and somatosensory vibrissal cortices
Article Snippet: .. Stereological axon measurements Stereological analyses were carried out with a BX61 light microscope (Olympus, Japan) equipped with a MT12 microcator (0.5-µm resolution; Heidenhain, Traunreut, Germany), a X–Y–Z high-precision motorized microscope stage (ProScan II; Prior Scientific, Cambridge, UK), and an Olympus DP-71 digital camera connected to a computer running the NewCAST stereology software package (Visiopharm, Hørsholm, Denmark). .. In samples from three different Po axon-labeling experiments, absolute axonal length in individual cortical layers of S1BF and M1wk was estimated using the isotropic virtual plane method following a fractionator sampling scheme (Drøjdahl et al. ).

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    Olympus bx61 light microscope
    Relocation of Rap1p ( a ) and Sir3p ( b ) from telomeres to the nucleolus and changes in expression patterns of Rap1p, Sir3p and Nop2p ( c ) during chronological aging. a, b After 2, 7 and 14 days, Rap1p ( a ) and Sir3p ( b ) immunostaining was performed ( green ). DNA was visualised using DAPI staining ( blue ). Immunostained cells were captured with an Olympus <t>BX61</t> light microscope equipped with a DP72 CCD camera and Olympus CellF software. Nop2p was used as a nucleolar marker ( red ). Typical micrographs of haploid wild type BY4741 are shown. c After 2 and 7 days, changes in the expression of Rap1p, Sir3p and Nop2p were revealed with western blotting. For the loading control, an antibody against actin was used. The chemiluminescence signal was detected using an ECL Plus Western Blotting Detection System (GE Healthcare) and G:BOX imaging system (Syngene). Top haploid strains, bottom diploid strains. (Color figure online)
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    Olympus bx61 fluorescent light microscope
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    Image Search Results


    Relocation of Rap1p ( a ) and Sir3p ( b ) from telomeres to the nucleolus and changes in expression patterns of Rap1p, Sir3p and Nop2p ( c ) during chronological aging. a, b After 2, 7 and 14 days, Rap1p ( a ) and Sir3p ( b ) immunostaining was performed ( green ). DNA was visualised using DAPI staining ( blue ). Immunostained cells were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. Nop2p was used as a nucleolar marker ( red ). Typical micrographs of haploid wild type BY4741 are shown. c After 2 and 7 days, changes in the expression of Rap1p, Sir3p and Nop2p were revealed with western blotting. For the loading control, an antibody against actin was used. The chemiluminescence signal was detected using an ECL Plus Western Blotting Detection System (GE Healthcare) and G:BOX imaging system (Syngene). Top haploid strains, bottom diploid strains. (Color figure online)

    Journal: Biogerontology

    Article Title: Links between nucleolar activity, rDNA stability, aneuploidy and chronological aging in the yeast Saccharomyces cerevisiae

    doi: 10.1007/s10522-014-9499-y

    Figure Lengend Snippet: Relocation of Rap1p ( a ) and Sir3p ( b ) from telomeres to the nucleolus and changes in expression patterns of Rap1p, Sir3p and Nop2p ( c ) during chronological aging. a, b After 2, 7 and 14 days, Rap1p ( a ) and Sir3p ( b ) immunostaining was performed ( green ). DNA was visualised using DAPI staining ( blue ). Immunostained cells were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. Nop2p was used as a nucleolar marker ( red ). Typical micrographs of haploid wild type BY4741 are shown. c After 2 and 7 days, changes in the expression of Rap1p, Sir3p and Nop2p were revealed with western blotting. For the loading control, an antibody against actin was used. The chemiluminescence signal was detected using an ECL Plus Western Blotting Detection System (GE Healthcare) and G:BOX imaging system (Syngene). Top haploid strains, bottom diploid strains. (Color figure online)

    Article Snippet: Silver staining of nucleolar argyrophilic proteins was conducted according to Howell and Black ( ) by incubating the cell slides with a colloidal developer containing 50 % AgNO3 in the dark at 37 °C for 15 min. After washing in tap water, the preparations were stained with 5 % Giemsa for 10 s and nucleoli were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software.

    Techniques: Expressing, Immunostaining, Staining, Light Microscopy, Software, Marker, Western Blot, Imaging

    CA-induced aneuploidy. Aneuploidy was assessed using fluorescence in situ hybridisation (FISH) using whole chromosome painting probes (WCPPs). After 2 and 28 days, cells were taken from chronological aging culture and transfer to fresh YPD medium to continue mitotic growth. A biotinylated probe specific to chromosome I or chromosome V (Polish Patent Office, registration number P.404526) was added to the spheroplast-coated slide and its detection was performed with a Star*FISH © Biotin Painting Kit—FITC Label (Cambio). Fluorescent signals were analysed using an Olympus BX61 fluorescence microscope equipped with a DP72 CCD camera and Olympus CellF software. a The frequencies of aneuploidy events (chromosome I or chromosome V): haploid strains ( left ), diploid strains ( right ). Aneuploidy events were counted and presented as a percentage of 200 total cell scores. b Typical micrographs showing chromosome I or chromosome V fluorescent signals are presented ( green ). DNA was visualised using DAPI staining ( blue ). (Color figure online)

    Journal: Biogerontology

    Article Title: Links between nucleolar activity, rDNA stability, aneuploidy and chronological aging in the yeast Saccharomyces cerevisiae

    doi: 10.1007/s10522-014-9499-y

    Figure Lengend Snippet: CA-induced aneuploidy. Aneuploidy was assessed using fluorescence in situ hybridisation (FISH) using whole chromosome painting probes (WCPPs). After 2 and 28 days, cells were taken from chronological aging culture and transfer to fresh YPD medium to continue mitotic growth. A biotinylated probe specific to chromosome I or chromosome V (Polish Patent Office, registration number P.404526) was added to the spheroplast-coated slide and its detection was performed with a Star*FISH © Biotin Painting Kit—FITC Label (Cambio). Fluorescent signals were analysed using an Olympus BX61 fluorescence microscope equipped with a DP72 CCD camera and Olympus CellF software. a The frequencies of aneuploidy events (chromosome I or chromosome V): haploid strains ( left ), diploid strains ( right ). Aneuploidy events were counted and presented as a percentage of 200 total cell scores. b Typical micrographs showing chromosome I or chromosome V fluorescent signals are presented ( green ). DNA was visualised using DAPI staining ( blue ). (Color figure online)

    Article Snippet: Silver staining of nucleolar argyrophilic proteins was conducted according to Howell and Black ( ) by incubating the cell slides with a colloidal developer containing 50 % AgNO3 in the dark at 37 °C for 15 min. After washing in tap water, the preparations were stained with 5 % Giemsa for 10 s and nucleoli were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software.

    Techniques: Fluorescence, In Situ, Hybridization, Fluorescence In Situ Hybridization, Microscopy, Software, Staining

    DNA breaks and structural aberrations within chromosome XII during chronological aging. After 2 and 14 days, yeast chromosomes were separated with PFGE and selected bands were cut with a razor blade. DNA breaks and structural aberrations within chromosome XII were visualised via alkaline chromosome comet assay (pH > 13). After electrophoresis, chromosomal DNA was stained with YOYO-1 (Molecular Probes) stain solution ( green ). a DNA breaks ( white arrowheads ) and structural aberrations (here termed as abnormal chromosome morphology) ( pink arrowheads ) were visualised using an Olympus BX61 fluorescence microscope equipped with a DP72 CCD camera and Olympus CellF software. Left haploid strains; right diploid strains; bottom positive control for DNA breaks, haploid wild type treated with 1 mM hydrogen peroxide for 10 min. b, c A total of 100 chromosomes per sample triplicate were analysed and the percentages of DNA damage and structural aberrations were calculated. b Haploid strains, c diploid strains. (Color figure online)

    Journal: Biogerontology

    Article Title: Links between nucleolar activity, rDNA stability, aneuploidy and chronological aging in the yeast Saccharomyces cerevisiae

    doi: 10.1007/s10522-014-9499-y

    Figure Lengend Snippet: DNA breaks and structural aberrations within chromosome XII during chronological aging. After 2 and 14 days, yeast chromosomes were separated with PFGE and selected bands were cut with a razor blade. DNA breaks and structural aberrations within chromosome XII were visualised via alkaline chromosome comet assay (pH > 13). After electrophoresis, chromosomal DNA was stained with YOYO-1 (Molecular Probes) stain solution ( green ). a DNA breaks ( white arrowheads ) and structural aberrations (here termed as abnormal chromosome morphology) ( pink arrowheads ) were visualised using an Olympus BX61 fluorescence microscope equipped with a DP72 CCD camera and Olympus CellF software. Left haploid strains; right diploid strains; bottom positive control for DNA breaks, haploid wild type treated with 1 mM hydrogen peroxide for 10 min. b, c A total of 100 chromosomes per sample triplicate were analysed and the percentages of DNA damage and structural aberrations were calculated. b Haploid strains, c diploid strains. (Color figure online)

    Article Snippet: Silver staining of nucleolar argyrophilic proteins was conducted according to Howell and Black ( ) by incubating the cell slides with a colloidal developer containing 50 % AgNO3 in the dark at 37 °C for 15 min. After washing in tap water, the preparations were stained with 5 % Giemsa for 10 s and nucleoli were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software.

    Techniques: Single Cell Gel Electrophoresis, Electrophoresis, Staining, Fluorescence, Microscopy, Software, Positive Control

    CA-mediated changes in nucleolus size and nucleolus/nucleus ratio. To visualise nucleoli, silver staining of nucleolar organiser regions (AgNOR) was performed and nucleoli images were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. The nucleolus size and nucleolus/nucleus ratio were calculated using ImageJ software http://rsbweb.nih.gov/ij/ . a Nucleolus size of chronologically aging haploid strains ( left ) and diploid strains ( right ). b Nucleolus/nucleus ratio of chronologically aging haploid strains ( left ) and diploid strains ( right )

    Journal: Biogerontology

    Article Title: Links between nucleolar activity, rDNA stability, aneuploidy and chronological aging in the yeast Saccharomyces cerevisiae

    doi: 10.1007/s10522-014-9499-y

    Figure Lengend Snippet: CA-mediated changes in nucleolus size and nucleolus/nucleus ratio. To visualise nucleoli, silver staining of nucleolar organiser regions (AgNOR) was performed and nucleoli images were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. The nucleolus size and nucleolus/nucleus ratio were calculated using ImageJ software http://rsbweb.nih.gov/ij/ . a Nucleolus size of chronologically aging haploid strains ( left ) and diploid strains ( right ). b Nucleolus/nucleus ratio of chronologically aging haploid strains ( left ) and diploid strains ( right )

    Article Snippet: Silver staining of nucleolar argyrophilic proteins was conducted according to Howell and Black ( ) by incubating the cell slides with a colloidal developer containing 50 % AgNO3 in the dark at 37 °C for 15 min. After washing in tap water, the preparations were stained with 5 % Giemsa for 10 s and nucleoli were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software.

    Techniques: Silver Staining, Light Microscopy, Software

    The nucleolus is fragmented during chronological aging. To visualise nucleoli, silver staining of nucleolar organiser regions (AgNOR) was performed and nucleoli images were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. a A scheme showing CA-mediated changes in nucleolar morphology. A general tendency is presented (both schematic and microscopic representations of the haploid and diploid wild type strain nucleoli are shown). b Graphs showing the percentage of normal ( black ), increased ( light grey ) and fragmented ( dark grey ) nucleoli in chronologically aging haploid strains ( left ) and diploid strains ( right ). A total of 100 cells were analysed, and their nucleolus morphological type was determined [%]. The results represent the mean from at least three independent experiments

    Journal: Biogerontology

    Article Title: Links between nucleolar activity, rDNA stability, aneuploidy and chronological aging in the yeast Saccharomyces cerevisiae

    doi: 10.1007/s10522-014-9499-y

    Figure Lengend Snippet: The nucleolus is fragmented during chronological aging. To visualise nucleoli, silver staining of nucleolar organiser regions (AgNOR) was performed and nucleoli images were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software. a A scheme showing CA-mediated changes in nucleolar morphology. A general tendency is presented (both schematic and microscopic representations of the haploid and diploid wild type strain nucleoli are shown). b Graphs showing the percentage of normal ( black ), increased ( light grey ) and fragmented ( dark grey ) nucleoli in chronologically aging haploid strains ( left ) and diploid strains ( right ). A total of 100 cells were analysed, and their nucleolus morphological type was determined [%]. The results represent the mean from at least three independent experiments

    Article Snippet: Silver staining of nucleolar argyrophilic proteins was conducted according to Howell and Black ( ) by incubating the cell slides with a colloidal developer containing 50 % AgNO3 in the dark at 37 °C for 15 min. After washing in tap water, the preparations were stained with 5 % Giemsa for 10 s and nucleoli were captured with an Olympus BX61 light microscope equipped with a DP72 CCD camera and Olympus CellF software.

    Techniques: Silver Staining, Light Microscopy, Software

    Fluorescence images: (a) Visualization of the nuclei of viable osteoblasts from the Control Group. Obtained with the Olympus BX61® fluorescence light microscope (Olympus Corporation, Shinjuku, Tokyo, Japan) and AxioScope® camera (Carl Zeiss®, OPM, Pico Dental, Germany) with a 20x objective. (b) Visualization of viable osteoblast nuclei of cultures on a machined surface. Images obtained with the Olympus BX61® fluorescence light microscope (Olympus Corporation, Shinjuku, Tokyo, Japan) and AxioScope® camera (Carl Zeiss®, OPM, Pico Dental, Germany) with a 20x objective. (c) Visualization of viable osteoblast nuclei of cultures on an Osseotite® surface. Images obtained with the Olympus BX61® fluorescence light microscope (Olympus Corporation, Shinjuku, Tokyo, Japan) and AxioScope® camera (Carl Zeiss®, OPM, Pico Dental, Germany) with a 20x objective. (d) Visualization of osteoblast cytoskeleton using rhodamine stain of cultured osteoblasts belonging to the Control Group. Images obtained with the Olympus BX61® fluorescence light microscope (Olympus Corporation, Shinjuku, Tokyo, Japan) and AxioScope® camera (Carl Zeiss®, OPM, Pico Dental, Germany) with a 20x objective. (e) Visualization of osteoblast cytoskeleton using rhodamine stain of osteoblasts cultured on a machined surface. Images obtained with the Olympus BX61® fluorescence light microscope (Olympus Corporation, Shinjuku, Tokyo, Japan) and AxioScope® camera (Carl Zeiss®, OPM, Pico Dental, Germany) with a 20x objective. (f) Visualization of osteoblast cytoskeleton using rhodamine stain of osteoblasts cultured on an Osseotite® surface. Images obtained with the Olympus BX61® fluorescence light microscope (Olympus Corporation, Shinjuku, Tokyo, Japan) and AxioScope® camera (Carl Zeiss®, OPM, Pico Dental, Germany) with a 20x objective. (g) Visualization of the Control Group cultured NHOst mitochondria with JC-1 visualized using the LEICA TCS-SP2 laser scanning confocal microscope (Leica Microsystems, Wetzlar, Germany) at 40x. (h) Visualization of NHOst mitochondria cultured on titanium discs with machined surface and stained with JC-1, visualized using the LEICA TCS-SP2 laser scanning confocal microscope (Leica Microsystems, Wetzlar, Germany) at 40x. (i) Visualization of NHOst mitochondria cultured on titanium discs treated with a double acid-etched nitric and hydrofluoric acid surface stained with JC-1 and visualized using the LEICA TCS-SP2 laser scanning confocal microscope (Leica Microsystems, Wetzlar, Germany) at 40x. (j) Visualization of BMP-2 digital images produced by cultured osteoblasts of the Control Group. Images obtained using a confocal microscope (Carl Zeiss®, OPM, Pico Dental, Germany) using a 635 helium-neon laser at 50%, with a 10x objective. (k) Visualization of BMP-2 digital images produced by osteoblasts cultured on a machined surface. Images obtained using a confocal microscope (Carl Zeiss®, OPM, Pico Dental, Germany) using a 635 helium-neon laser at 50%, with a 10x objective. (l) Visualization of BMP-2 digital images produced by osteoblasts cultured on an Osseotite® surface. Images obtained using a confocal microscope (Carl Zeiss®, OPM, Pico Dental, Germany) using a 635 helium-neon laser at 50%, with a 10x objective.

    Journal: Medicina Oral, Patología Oral y Cirugía Bucal

    Article Title: Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium

    doi: 10.4317/medoral.22071

    Figure Lengend Snippet: Fluorescence images: (a) Visualization of the nuclei of viable osteoblasts from the Control Group. Obtained with the Olympus BX61® fluorescence light microscope (Olympus Corporation, Shinjuku, Tokyo, Japan) and AxioScope® camera (Carl Zeiss®, OPM, Pico Dental, Germany) with a 20x objective. (b) Visualization of viable osteoblast nuclei of cultures on a machined surface. Images obtained with the Olympus BX61® fluorescence light microscope (Olympus Corporation, Shinjuku, Tokyo, Japan) and AxioScope® camera (Carl Zeiss®, OPM, Pico Dental, Germany) with a 20x objective. (c) Visualization of viable osteoblast nuclei of cultures on an Osseotite® surface. Images obtained with the Olympus BX61® fluorescence light microscope (Olympus Corporation, Shinjuku, Tokyo, Japan) and AxioScope® camera (Carl Zeiss®, OPM, Pico Dental, Germany) with a 20x objective. (d) Visualization of osteoblast cytoskeleton using rhodamine stain of cultured osteoblasts belonging to the Control Group. Images obtained with the Olympus BX61® fluorescence light microscope (Olympus Corporation, Shinjuku, Tokyo, Japan) and AxioScope® camera (Carl Zeiss®, OPM, Pico Dental, Germany) with a 20x objective. (e) Visualization of osteoblast cytoskeleton using rhodamine stain of osteoblasts cultured on a machined surface. Images obtained with the Olympus BX61® fluorescence light microscope (Olympus Corporation, Shinjuku, Tokyo, Japan) and AxioScope® camera (Carl Zeiss®, OPM, Pico Dental, Germany) with a 20x objective. (f) Visualization of osteoblast cytoskeleton using rhodamine stain of osteoblasts cultured on an Osseotite® surface. Images obtained with the Olympus BX61® fluorescence light microscope (Olympus Corporation, Shinjuku, Tokyo, Japan) and AxioScope® camera (Carl Zeiss®, OPM, Pico Dental, Germany) with a 20x objective. (g) Visualization of the Control Group cultured NHOst mitochondria with JC-1 visualized using the LEICA TCS-SP2 laser scanning confocal microscope (Leica Microsystems, Wetzlar, Germany) at 40x. (h) Visualization of NHOst mitochondria cultured on titanium discs with machined surface and stained with JC-1, visualized using the LEICA TCS-SP2 laser scanning confocal microscope (Leica Microsystems, Wetzlar, Germany) at 40x. (i) Visualization of NHOst mitochondria cultured on titanium discs treated with a double acid-etched nitric and hydrofluoric acid surface stained with JC-1 and visualized using the LEICA TCS-SP2 laser scanning confocal microscope (Leica Microsystems, Wetzlar, Germany) at 40x. (j) Visualization of BMP-2 digital images produced by cultured osteoblasts of the Control Group. Images obtained using a confocal microscope (Carl Zeiss®, OPM, Pico Dental, Germany) using a 635 helium-neon laser at 50%, with a 10x objective. (k) Visualization of BMP-2 digital images produced by osteoblasts cultured on a machined surface. Images obtained using a confocal microscope (Carl Zeiss®, OPM, Pico Dental, Germany) using a 635 helium-neon laser at 50%, with a 10x objective. (l) Visualization of BMP-2 digital images produced by osteoblasts cultured on an Osseotite® surface. Images obtained using a confocal microscope (Carl Zeiss®, OPM, Pico Dental, Germany) using a 635 helium-neon laser at 50%, with a 10x objective.

    Article Snippet: After staining, images were captured using the Olympus BX61® fluorescent light microscope (Olympus Corporation, Shinjuku, Tokyo, Japan) and the AxioScope® camera (Carl Zeiss®, OPM, Pico Dental, Germany) with a 20x objective and a x2.3 zoom.

    Techniques: Fluorescence, Light Microscopy, Staining, Cell Culture, Microscopy, Produced

    Multi‐color fluorescence mini‐endoscopic system and near infrared photoimmunotherapy ( NIR ‐ PIT ) procedure. A, Multi‐color fluorescence imaging system is based on a clinically available fiber optic endoscope and light source. Excitation light is provided by multi‐band excitation filters. Endoscopic images were obtained via a beam splitter, where the white light images were detected using the color‐ CCD camera and the fluorescence images were filtered by multi‐color emission filters and detected with an ( EM )‐ CCD camera. Both images are displayed side by side on the monitor. B, Cylindrical light diffuser with an NIR laser system was used. The cylindrical light diffuser was used through the endoscope. C, The endoscope was inserted into abdominal cavity through a small lower abdominal incision, and the abdominal cavity was inflated with air. Peritoneal cavity was exposed to NIR laser light using the optical diffuser under endoscopic guidance

    Journal: Cancer Science

    Article Title: Endoscopic near infrared photoimmunotherapy using a fiber optic diffuser for peritoneal dissemination of gastric cancer. Endoscopic near infrared photoimmunotherapy using a fiber optic diffuser for peritoneal dissemination of gastric cancer

    doi: 10.1111/cas.13621

    Figure Lengend Snippet: Multi‐color fluorescence mini‐endoscopic system and near infrared photoimmunotherapy ( NIR ‐ PIT ) procedure. A, Multi‐color fluorescence imaging system is based on a clinically available fiber optic endoscope and light source. Excitation light is provided by multi‐band excitation filters. Endoscopic images were obtained via a beam splitter, where the white light images were detected using the color‐ CCD camera and the fluorescence images were filtered by multi‐color emission filters and detected with an ( EM )‐ CCD camera. Both images are displayed side by side on the monitor. B, Cylindrical light diffuser with an NIR laser system was used. The cylindrical light diffuser was used through the endoscope. C, The endoscope was inserted into abdominal cavity through a small lower abdominal incision, and the abdominal cavity was inflated with air. Peritoneal cavity was exposed to NIR laser light using the optical diffuser under endoscopic guidance

    Article Snippet: Paraffin‐embedded sections were stained with H & E. Post NIR‐PIT tissue microscopy (BX61; Olympus America, Melville, NY, USA) was then performed.

    Techniques: Fluorescence, Imaging

    Endoscopic near infrared photoimmunotherapy ( NIR ‐ PIT ) and in vivo effect of NIR ‐ PIT in peritoneal N87 GFP ‐luc tumors. A, NIR ‐ PIT regimen. Endoscopic and bioluminescence images were obtained at each time point as indicated. B, In vivo fluorescence real‐time endoscopic intraperitoneal imaging of N87 GFP ‐luc tumor‐bearing mice. After NIR ‐ PIT IR 700 fluorescence decreased. In contrast, no changes in GFP fluorescence were shown. C, In vivo bioluminescence imaging ( BLI ) of tumor‐bearing mice in response to NIR ‐ PIT . Before NIR ‐ PIT , tumors exhibited high signals on BLI . The tumors treated by NIR ‐ PIT showed a marked decrease in BLI . D, Quantitative luciferase activity (before NIR ‐ PIT is set to 100) showed a significant decrease in endoscopic NIR ‐ PIT groups compared with the 3 control groups (n ≥ 10, ** P

    Journal: Cancer Science

    Article Title: Endoscopic near infrared photoimmunotherapy using a fiber optic diffuser for peritoneal dissemination of gastric cancer. Endoscopic near infrared photoimmunotherapy using a fiber optic diffuser for peritoneal dissemination of gastric cancer

    doi: 10.1111/cas.13621

    Figure Lengend Snippet: Endoscopic near infrared photoimmunotherapy ( NIR ‐ PIT ) and in vivo effect of NIR ‐ PIT in peritoneal N87 GFP ‐luc tumors. A, NIR ‐ PIT regimen. Endoscopic and bioluminescence images were obtained at each time point as indicated. B, In vivo fluorescence real‐time endoscopic intraperitoneal imaging of N87 GFP ‐luc tumor‐bearing mice. After NIR ‐ PIT IR 700 fluorescence decreased. In contrast, no changes in GFP fluorescence were shown. C, In vivo bioluminescence imaging ( BLI ) of tumor‐bearing mice in response to NIR ‐ PIT . Before NIR ‐ PIT , tumors exhibited high signals on BLI . The tumors treated by NIR ‐ PIT showed a marked decrease in BLI . D, Quantitative luciferase activity (before NIR ‐ PIT is set to 100) showed a significant decrease in endoscopic NIR ‐ PIT groups compared with the 3 control groups (n ≥ 10, ** P

    Article Snippet: Paraffin‐embedded sections were stained with H & E. Post NIR‐PIT tissue microscopy (BX61; Olympus America, Melville, NY, USA) was then performed.

    Techniques: In Vivo, Fluorescence, Imaging, Mouse Assay, Luciferase, Activity Assay

    In vivo fluorescence real‐time endoscopic imaging and histological near infrared photoimmunotherapy ( NIR ‐ PIT ) effect. A, Treatment regimen is shown. Biopsy specimens were obtained 6 hours after NIR ‐ PIT . B, In vivo real‐time endoscopic intraperitoneal imaging of N87 GFP ‐luc tumor‐bearing mice. Peritoneal cavity was exposed to NIR light using an optical diffuser through the endoscope. C, In vivo fluorescence real‐time endoscopic intraperitoneal imaging of N87 GFP ‐luc tumor‐bearing mice. Tumors demonstrate GFP and IR 700 fluorescence in mice administered tra‐ IR 700. In the absence of tra‐ IR 700 no IR 700 fluorescence was seen. After NIR ‐ PIT , IR 700 fluorescence decreased. D, Biopsy specimens stained with H E demonstrate few scattered clusters of damaged tumor cells within a background of diffuse cellular necrosis and micro‐hemorrhage with infiltration of inflammatory cells consistent with acute granulation in endoscopic NIR‐PIT group, while no obvious damage was observed in control groups, including tra‐ IR 700 alone without NIR light and NIR light alone without tra‐ IR 700 groups. Yellow scale bars = 100 μm. Black scale bars = 20 μm

    Journal: Cancer Science

    Article Title: Endoscopic near infrared photoimmunotherapy using a fiber optic diffuser for peritoneal dissemination of gastric cancer. Endoscopic near infrared photoimmunotherapy using a fiber optic diffuser for peritoneal dissemination of gastric cancer

    doi: 10.1111/cas.13621

    Figure Lengend Snippet: In vivo fluorescence real‐time endoscopic imaging and histological near infrared photoimmunotherapy ( NIR ‐ PIT ) effect. A, Treatment regimen is shown. Biopsy specimens were obtained 6 hours after NIR ‐ PIT . B, In vivo real‐time endoscopic intraperitoneal imaging of N87 GFP ‐luc tumor‐bearing mice. Peritoneal cavity was exposed to NIR light using an optical diffuser through the endoscope. C, In vivo fluorescence real‐time endoscopic intraperitoneal imaging of N87 GFP ‐luc tumor‐bearing mice. Tumors demonstrate GFP and IR 700 fluorescence in mice administered tra‐ IR 700. In the absence of tra‐ IR 700 no IR 700 fluorescence was seen. After NIR ‐ PIT , IR 700 fluorescence decreased. D, Biopsy specimens stained with H E demonstrate few scattered clusters of damaged tumor cells within a background of diffuse cellular necrosis and micro‐hemorrhage with infiltration of inflammatory cells consistent with acute granulation in endoscopic NIR‐PIT group, while no obvious damage was observed in control groups, including tra‐ IR 700 alone without NIR light and NIR light alone without tra‐ IR 700 groups. Yellow scale bars = 100 μm. Black scale bars = 20 μm

    Article Snippet: Paraffin‐embedded sections were stained with H & E. Post NIR‐PIT tissue microscopy (BX61; Olympus America, Melville, NY, USA) was then performed.

    Techniques: In Vivo, Fluorescence, Imaging, Mouse Assay, Staining

    Microscopy images of skin penetration studies performed on ex vivo human skin from a patient with IBC. (A) Brightfield image of a skin after microneedle insertion for 10 s. (B) Fluorescence image after microneedle insertion for 10 min. The dotted line

    Journal: Advanced materials (Deerfield Beach, Fla.)

    Article Title: Rapidly–Dissolvable Microneedle Patches Via a Highly Scalable and Reproducible Soft Lithography Approach

    doi: 10.1002/adma.201300526

    Figure Lengend Snippet: Microscopy images of skin penetration studies performed on ex vivo human skin from a patient with IBC. (A) Brightfield image of a skin after microneedle insertion for 10 s. (B) Fluorescence image after microneedle insertion for 10 min. The dotted line

    Article Snippet: The remaining sections were H & E stained (CRYO-KIT, Cancer Diagnostics) for brightfield microscopy imaging (Olympus BX61 Upright Brightfield Microscope).

    Techniques: Microscopy, Ex Vivo, Fluorescence

    Brightfield macroscopic images of a microneedle patch. (A) The microneedle array morphology, showing reproducible needles. Scale bar is 200 μm. (B) A curled microneedle array, showing the flexibility of the array. Scale bar is 1 cm. (C) A side

    Journal: Advanced materials (Deerfield Beach, Fla.)

    Article Title: Rapidly–Dissolvable Microneedle Patches Via a Highly Scalable and Reproducible Soft Lithography Approach

    doi: 10.1002/adma.201300526

    Figure Lengend Snippet: Brightfield macroscopic images of a microneedle patch. (A) The microneedle array morphology, showing reproducible needles. Scale bar is 200 μm. (B) A curled microneedle array, showing the flexibility of the array. Scale bar is 1 cm. (C) A side

    Article Snippet: The remaining sections were H & E stained (CRYO-KIT, Cancer Diagnostics) for brightfield microscopy imaging (Olympus BX61 Upright Brightfield Microscope).

    Techniques:

    Images of ex vivo murine skin after testing with PRINT microneedles. (A) Brightfield macroscopic image after testing with microneedle patch for 10 seconds. The pattern of the microneedles can be seen on the skin. In the insert, a single piercing is highlighted.

    Journal: Advanced materials (Deerfield Beach, Fla.)

    Article Title: Rapidly–Dissolvable Microneedle Patches Via a Highly Scalable and Reproducible Soft Lithography Approach

    doi: 10.1002/adma.201300526

    Figure Lengend Snippet: Images of ex vivo murine skin after testing with PRINT microneedles. (A) Brightfield macroscopic image after testing with microneedle patch for 10 seconds. The pattern of the microneedles can be seen on the skin. In the insert, a single piercing is highlighted.

    Article Snippet: The remaining sections were H & E stained (CRYO-KIT, Cancer Diagnostics) for brightfield microscopy imaging (Olympus BX61 Upright Brightfield Microscope).

    Techniques: Ex Vivo