bx51 light microscope  (Olympus)

 
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    BX61WI BX51WI Fixed Stage Microscope
    Description:
    A Dual Commitment for Physiological Experiments The BX51WI is ideal for all physiological experiments such as patch clamping and intravital microscopy The fixed stage concept and vibration free frame design ensure excellent stability throughout the experiment Use of infrared light protects living cells and offers high penetration depths of thick tissue slices while high NA optics allow magnification changes without moving the objective The BX61WI is the motorized version of the BX51WI fixed stage microscope with a highly accurate Z drive It is the ideal tool for all automated physiological experiments such as patch clamping and intravital microscopy
    Catalog Number:
    BX61WI-BX51WI-FIXED-STAGE-MICROSCOPE
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    Products Upright Microscopes Electrophysiology Microscopes Fixed Stage Microscope
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    Structured Review

    Olympus bx51 light microscope
    BX61WI BX51WI Fixed Stage Microscope
    A Dual Commitment for Physiological Experiments The BX51WI is ideal for all physiological experiments such as patch clamping and intravital microscopy The fixed stage concept and vibration free frame design ensure excellent stability throughout the experiment Use of infrared light protects living cells and offers high penetration depths of thick tissue slices while high NA optics allow magnification changes without moving the objective The BX61WI is the motorized version of the BX51WI fixed stage microscope with a highly accurate Z drive It is the ideal tool for all automated physiological experiments such as patch clamping and intravital microscopy
    https://www.bioz.com/result/bx51 light microscope/product/Olympus
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bx51 light microscope - by Bioz Stars, 2021-05
    97/100 stars

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    Related Articles

    Microscopy:

    Article Title: Temperature effects on synaptic transmission and neuronal function in the visual thalamus
    Article Snippet: After incubating in the NMDG solution for 10 minutes, slices were transferred to a nylon net in a beaker containing room temperature aCSF and allowed to recover for > 1 hour prior to patch clamp experiments. .. For electrophysiology experiments, slices were positioned in a recording chamber on an upright fixed-stage microscope (Olympus BX51WI) and superfused with aCSF supplemented with 60 μM picrotoxin at ~2–4 mL/min. .. Temperature was varied from room temperature (~22–24°C) to warmed bath conditions (~31–34°C) using an in-line solution heater (Warner Instruments) and measured in the bath near the slice.

    Article Title: Endocannabinoid Signaling at Hypothalamic Steroidogenic Factor-1/Proopiomelanocortin Synapses Is Sex- and Diet-Sensitive
    Article Snippet: The signals underwent analog-digital conversion via a Digidata 1322A interface coupled to pClamp 10.5 software (Axon instruments). .. For the transgenic mouse experiments, recordings were made using an Olympus BX51 W1 fixed stage microscope outfitted with infrared differential interference contrast video imaging. .. A Multiclamp 700B preamplifier (Molecular Devices) amplified potentials and passed current through the electrode.

    Article Title: Circadian modulation of neurons and astrocytes controls synaptic plasticity in hippocampal area CA1
    Article Snippet: .. We identified the hippocampus under bright field illumination using an upright fixed-stage microscope (BX51 WI; Olympus Corporation, Center Valley, PA). ..

    Article Title: Bovine sperm-oviduct interactions are characterized by specific sperm behaviour, ultrastructure and tubal reactions which are impacted by sex sorting
    Article Snippet: .. The Delta-T dish was then placed onto a BX51 WI fixed-stage upright microscope (Olympus, Hamburg, Germany) with a specific Delta-T dish holder coupled to a stage and objective heater (Bioptechs Inc., Philadelphia, USA). .. Spermatozoa were classified as either agile (binding at tangential angle of about 30° with an actively beating, undulating tail movement), lagging (binding at lower angle, reduced tail movement at low frequency), immotile (lying flat on cilia, lack of tail movement) or hyperactivated (binding at tangential angle, rotation of the head, whip-like tail movements).

    Article Title: Release from the cone ribbon synapse under bright light conditions can be controlled by the opening of only a few Ca2+ channels
    Article Snippet: .. Retinal slices were rotated 90° to see the retinal layers on an upright fixed-stage microscope (Olympus BHWI or Nikon E600FN, Tokyo, Japan) using a water immersion objective [×40, 0.7 numerical aperture (NA) or ×60, 1.0 NA]. .. Cones were voltage clamped simultaneously with adjacent postsynaptic horizontal cells with a Multiclamp 700A amplifier (Molecular Devices, Sunnyvale, CA).

    Article Title: V3 Interneurons Regulate Locomotor Vigor by Recruitment of Spinal Motor Neurons During Fictive Swimming in Larval Zebrafish
    Article Snippet: For calcium imaging of MNs, Calcium Green-1 AM (Life Technologies, Grand Island, NY) dye was loaded into MNs using the protocol for Rhod-2 AM dye loading described above. .. Larvae were transferred to a fixed-stage Olympus BX51 WI upright microscope with either a 20x / NA 0.5 or a 40x / NA 0.8 water dipping objective lens. .. A field of view containing GCaMP6s-expressing V3-INs in transgenic larvae or Calcium Green-1 AM-loaded MNs in wild type larvae was selected for calcium imaging and a simultaneous peripheral nerve (PN) recording was acquired.

    Article Title: Transduction of group I mGluR-mediated synaptic plasticity by β-arrestin2 signalling
    Article Snippet: Following incubation, individual slices were transferred to a recording chamber and continuously perfused with oxygenated aCSF at room temperature (25 °C). .. Hippocampal CA1 and CA3 pyramidal neurons were visually identified using a BX51WI fixed-stage upright microscope (Olympus, Center Valley, PA) and subsequently used for whole-cell patch clamp recordings with a MultiClamp 700A amplifier (Molecular Devices, Sunnyvale, CA). ..

    Article Title: Electrokinetically-Driven Assembly of Gold Colloids into Nanostructures for Surface-Enhanced Raman Scattering
    Article Snippet: .. Fluorescence microscopy was performed on an Olympus BX fixed stage microscope with a Cy3 filter (Olympus, Richmond Hill, ON, Canada). ..

    Transgenic Assay:

    Article Title: Endocannabinoid Signaling at Hypothalamic Steroidogenic Factor-1/Proopiomelanocortin Synapses Is Sex- and Diet-Sensitive
    Article Snippet: The signals underwent analog-digital conversion via a Digidata 1322A interface coupled to pClamp 10.5 software (Axon instruments). .. For the transgenic mouse experiments, recordings were made using an Olympus BX51 W1 fixed stage microscope outfitted with infrared differential interference contrast video imaging. .. A Multiclamp 700B preamplifier (Molecular Devices) amplified potentials and passed current through the electrode.

    Imaging:

    Article Title: Endocannabinoid Signaling at Hypothalamic Steroidogenic Factor-1/Proopiomelanocortin Synapses Is Sex- and Diet-Sensitive
    Article Snippet: The signals underwent analog-digital conversion via a Digidata 1322A interface coupled to pClamp 10.5 software (Axon instruments). .. For the transgenic mouse experiments, recordings were made using an Olympus BX51 W1 fixed stage microscope outfitted with infrared differential interference contrast video imaging. .. A Multiclamp 700B preamplifier (Molecular Devices) amplified potentials and passed current through the electrode.

    Patch Clamp:

    Article Title: Transduction of group I mGluR-mediated synaptic plasticity by β-arrestin2 signalling
    Article Snippet: Following incubation, individual slices were transferred to a recording chamber and continuously perfused with oxygenated aCSF at room temperature (25 °C). .. Hippocampal CA1 and CA3 pyramidal neurons were visually identified using a BX51WI fixed-stage upright microscope (Olympus, Center Valley, PA) and subsequently used for whole-cell patch clamp recordings with a MultiClamp 700A amplifier (Molecular Devices, Sunnyvale, CA). ..

    Fluorescence:

    Article Title: Electrokinetically-Driven Assembly of Gold Colloids into Nanostructures for Surface-Enhanced Raman Scattering
    Article Snippet: .. Fluorescence microscopy was performed on an Olympus BX fixed stage microscope with a Cy3 filter (Olympus, Richmond Hill, ON, Canada). ..

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    Olympus bx51 light microscope
    Aged mice develop emphysema and airway remodeling after 3 months of CS exposure A. Lung function measurements for dynamic lung compliance were performed in intubated animals after 2 months of CS exposure and in tracheostomized animals after 3 months of CS exposure. B. Representative micrographs of HE-stained lung tissue sections from young and old FA and CS-exposed mice; scale bar 200 μm. C. Quantitative measurement of emphysema was determined by design-based stereology of HE-stained lung tissue sections using an Olympus <t>BX51</t> light microscope equipped with the computer-assisted stereological toolbox newCAST. D. Representative micrographs of Masson's Trichrome staining of lung tissue sections from young and old FA and CS-exposed mice; scale bar 100 μm. E. Total volume of airway collagen per basal membrane was determined via quantitative morphological assessment. Data were combined from 2 independent experiments with n = 8 and are given as mean values ± SD; one-way ANOVA following Bonferroni post test with ** p
    Bx51 Light Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bx51 light microscope/product/Olympus
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bx51 light microscope - by Bioz Stars, 2021-05
    97/100 stars
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    Aged mice develop emphysema and airway remodeling after 3 months of CS exposure A. Lung function measurements for dynamic lung compliance were performed in intubated animals after 2 months of CS exposure and in tracheostomized animals after 3 months of CS exposure. B. Representative micrographs of HE-stained lung tissue sections from young and old FA and CS-exposed mice; scale bar 200 μm. C. Quantitative measurement of emphysema was determined by design-based stereology of HE-stained lung tissue sections using an Olympus BX51 light microscope equipped with the computer-assisted stereological toolbox newCAST. D. Representative micrographs of Masson's Trichrome staining of lung tissue sections from young and old FA and CS-exposed mice; scale bar 100 μm. E. Total volume of airway collagen per basal membrane was determined via quantitative morphological assessment. Data were combined from 2 independent experiments with n = 8 and are given as mean values ± SD; one-way ANOVA following Bonferroni post test with ** p

    Journal: Oncotarget

    Article Title: Inflammaging increases susceptibility to cigarette smoke-induced COPD

    doi: 10.18632/oncotarget.4027

    Figure Lengend Snippet: Aged mice develop emphysema and airway remodeling after 3 months of CS exposure A. Lung function measurements for dynamic lung compliance were performed in intubated animals after 2 months of CS exposure and in tracheostomized animals after 3 months of CS exposure. B. Representative micrographs of HE-stained lung tissue sections from young and old FA and CS-exposed mice; scale bar 200 μm. C. Quantitative measurement of emphysema was determined by design-based stereology of HE-stained lung tissue sections using an Olympus BX51 light microscope equipped with the computer-assisted stereological toolbox newCAST. D. Representative micrographs of Masson's Trichrome staining of lung tissue sections from young and old FA and CS-exposed mice; scale bar 100 μm. E. Total volume of airway collagen per basal membrane was determined via quantitative morphological assessment. Data were combined from 2 independent experiments with n = 8 and are given as mean values ± SD; one-way ANOVA following Bonferroni post test with ** p

    Article Snippet: Quantitative morphometry Design-based stereology was used to analyze sections using an Olympus BX51 light microscope equipped with a computer-assisted stereological toolbox (newCAST, Visiopharm, Hoersholm, Denmark) on HE- or Masson's Trichrome stained lung tissue slides as previously described [ ].

    Techniques: Mouse Assay, Staining, Light Microscopy

    Effect of T. cruzi infection of enteric neuron cultures on neuronal bodies and neurite network. Neurons removed from the myenteric plexus were dissociated, plated, and infected or not with trypomastigotes of the T. cruzi strains Y and Dm28c. Cultures were analyzed at 24, 48, and 72 h post-infection (h.p.i.) after they were fixed using a 4% buffered paraformaldehyde solution. The coverslips were photographed in phase contrast [ (A) , control (CTRL) and (C) , infected]; stained with Giemsa [ (B) , CTRL and (D) , infected]; immunostained with anti-PGP 9.5 [in green; (E) , CTRL and (F – H) , infected] and anti-β-tubulin III [in red; (I) , CTRL and (J) , infected]; (H) Nuclear staining with Hoechst 33342 (in blue). Notice in the inset the presence of Hoechst fluorescent amastigotes labeled with PGP 9.5 inside neuronal cell bodies (arrowheads); β-tubulin and nNOS double-stained neurons (arrowheads) and correspondent phase-contrast images [ (K–M) , CTRL; (N–P) , infected]; (O,P) confocal images, with arrows indicating β-tubulin stained neurons. The double nNOS and β-tubulin infected neurons are indicated by arrowheads. The inset shows Hoechst fluorescent (in blue) amastigotes inside the neurons (arrowheads). (A,C) 10X objective; (B,D,G,H) 20X objective. (E,F,I,J) 40X objective. Scale bar: 3 μm. (K-P) 40X objective. Scale bar: 25 μm (Q) Fluorescence intensity of anti-β-tubulin III labeled neuronal bodies; (R) Fluorescence intensity of anti-β-tubulin III labeled neurites ( n = 4). Representative of two independent experiments in duplicate coverslips). Images were obtained an Olympus BX51 fluorescence microscope, 40X objective. Statistical analysis: Student t -test. Difference in relation to the control group, P

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Neuronal Parasitism, Early Myenteric Neurons Depopulation and Continuous Axonal Networking Damage as Underlying Mechanisms of the Experimental Intestinal Chagas' Disease

    doi: 10.3389/fcimb.2020.583899

    Figure Lengend Snippet: Effect of T. cruzi infection of enteric neuron cultures on neuronal bodies and neurite network. Neurons removed from the myenteric plexus were dissociated, plated, and infected or not with trypomastigotes of the T. cruzi strains Y and Dm28c. Cultures were analyzed at 24, 48, and 72 h post-infection (h.p.i.) after they were fixed using a 4% buffered paraformaldehyde solution. The coverslips were photographed in phase contrast [ (A) , control (CTRL) and (C) , infected]; stained with Giemsa [ (B) , CTRL and (D) , infected]; immunostained with anti-PGP 9.5 [in green; (E) , CTRL and (F – H) , infected] and anti-β-tubulin III [in red; (I) , CTRL and (J) , infected]; (H) Nuclear staining with Hoechst 33342 (in blue). Notice in the inset the presence of Hoechst fluorescent amastigotes labeled with PGP 9.5 inside neuronal cell bodies (arrowheads); β-tubulin and nNOS double-stained neurons (arrowheads) and correspondent phase-contrast images [ (K–M) , CTRL; (N–P) , infected]; (O,P) confocal images, with arrows indicating β-tubulin stained neurons. The double nNOS and β-tubulin infected neurons are indicated by arrowheads. The inset shows Hoechst fluorescent (in blue) amastigotes inside the neurons (arrowheads). (A,C) 10X objective; (B,D,G,H) 20X objective. (E,F,I,J) 40X objective. Scale bar: 3 μm. (K-P) 40X objective. Scale bar: 25 μm (Q) Fluorescence intensity of anti-β-tubulin III labeled neuronal bodies; (R) Fluorescence intensity of anti-β-tubulin III labeled neurites ( n = 4). Representative of two independent experiments in duplicate coverslips). Images were obtained an Olympus BX51 fluorescence microscope, 40X objective. Statistical analysis: Student t -test. Difference in relation to the control group, P

    Article Snippet: Obtaining ImagesFor photographic documentation and to obtain the images needed for morphometric analysis, the slides were photographed using the Olympus BX51 direct light optical microscope equipped with Image-Pro Express 4.0 software (Media Cybernetics, USA) with a resolution of 1,392 × 1,040 pixels.

    Techniques: Infection, Staining, Labeling, Fluorescence, Microscopy

    Mortality curve, infection parameters, and characterization of the inflammation associated with parasitism throughout the infection. Swiss female mice were infected with 50,000 T. cruzi strain Y trypomastigotes. (A) Survival curve: The mice in the control group and those treated with benznidazole (BZ) were followed throughout the time of infection. Control animals (no treatment) showed 100% mortality up to 20 d.p.i.; (B) Inflammatory foci: following HandE staining, 30 fields presenting inflammatory foci in the colon of infected mice were analyzed using an Olympus BX51 optical microscope with a 20X objective. Inflammatory foci were identified by the number of cells (at least 10 cells) and counted for the IAP, ICP3, ICP7, ICP12, and ICP15 groups. ( n = 3 mice). Data are representative of two independent experiments. Statistical analysis: ANOVA one-way with Tukey's post-hoc tests, after logarithmic transformation. Difference in relation to the infected acute phase group (IAP), P ≤ 0.05 (#). Data are shown as mean and standard deviation (SD); (C) The peak of parasitemia was reached at 8 d.p.i.; (D) Sagittal section of the intestine showing the IAP (upper panel) and chronic infected (ICP15.; lower panel) stages of infection. The distribution of inflammation in the acute phase presents as diffuse, coalescent foci whereas in the chronic phase, it persists as smaller foci. The thickness of the inner muscular layer was measured from the submucosal layer to the inner edge of the outer muscular layer (black line; Scale bar: 10 μm. 1X and 5X objective; the Sharp symbol indicates the width of the inner muscular layer); (E) DNA of the parasite: a sample of ~0.5 cm was taken from the proximal and distal colon of acute (IAP) and chronic (ICP3, ICP7, ICP12, ICP15) mice. Tissues were macerated and DNA extraction was performed, followed by PCR to amplify the parasite's DNA. ( n = 9–16 mice). Data are representative of two independent experiments. Statistical analysis: one-way ANOVA with Student-Newman-Keuls pos-hoc test. Time difference from acute (IAP), P

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Neuronal Parasitism, Early Myenteric Neurons Depopulation and Continuous Axonal Networking Damage as Underlying Mechanisms of the Experimental Intestinal Chagas' Disease

    doi: 10.3389/fcimb.2020.583899

    Figure Lengend Snippet: Mortality curve, infection parameters, and characterization of the inflammation associated with parasitism throughout the infection. Swiss female mice were infected with 50,000 T. cruzi strain Y trypomastigotes. (A) Survival curve: The mice in the control group and those treated with benznidazole (BZ) were followed throughout the time of infection. Control animals (no treatment) showed 100% mortality up to 20 d.p.i.; (B) Inflammatory foci: following HandE staining, 30 fields presenting inflammatory foci in the colon of infected mice were analyzed using an Olympus BX51 optical microscope with a 20X objective. Inflammatory foci were identified by the number of cells (at least 10 cells) and counted for the IAP, ICP3, ICP7, ICP12, and ICP15 groups. ( n = 3 mice). Data are representative of two independent experiments. Statistical analysis: ANOVA one-way with Tukey's post-hoc tests, after logarithmic transformation. Difference in relation to the infected acute phase group (IAP), P ≤ 0.05 (#). Data are shown as mean and standard deviation (SD); (C) The peak of parasitemia was reached at 8 d.p.i.; (D) Sagittal section of the intestine showing the IAP (upper panel) and chronic infected (ICP15.; lower panel) stages of infection. The distribution of inflammation in the acute phase presents as diffuse, coalescent foci whereas in the chronic phase, it persists as smaller foci. The thickness of the inner muscular layer was measured from the submucosal layer to the inner edge of the outer muscular layer (black line; Scale bar: 10 μm. 1X and 5X objective; the Sharp symbol indicates the width of the inner muscular layer); (E) DNA of the parasite: a sample of ~0.5 cm was taken from the proximal and distal colon of acute (IAP) and chronic (ICP3, ICP7, ICP12, ICP15) mice. Tissues were macerated and DNA extraction was performed, followed by PCR to amplify the parasite's DNA. ( n = 9–16 mice). Data are representative of two independent experiments. Statistical analysis: one-way ANOVA with Student-Newman-Keuls pos-hoc test. Time difference from acute (IAP), P

    Article Snippet: Obtaining ImagesFor photographic documentation and to obtain the images needed for morphometric analysis, the slides were photographed using the Olympus BX51 direct light optical microscope equipped with Image-Pro Express 4.0 software (Media Cybernetics, USA) with a resolution of 1,392 × 1,040 pixels.

    Techniques: Infection, Mouse Assay, Staining, Microscopy, Transformation Assay, Standard Deviation, DNA Extraction, Polymerase Chain Reaction

    Reactive oxygen species (ROS) accumulation upon wounding and flg22 induction is altered in omg1 leaves compared to Col-0 leaves. (A and B) DCF-DA ROS assay performed on wounded leaf tissues reveals that OMG1 affects cellular ROS production. (A) DCF-DA is a fluorogenic dye that measures hydroxyl, peroxyl and other ROS activity within the cell. A pipette tip was used to wound the surface of the leaves from the WT line, omg1 KO line and rbohd KO line (a known ROS-deficient mutant). Upon wounding, ROS is usually produced around the site of injury. The DCF-DA reagent is then oxidized by ROS resulting in appearance of green fluorescence. Plants were wounded and immediately placed in the DCF-DA solution for 20 min before visualized on the light microscope. (i) WT Col-0 leaves without wounding, (ii) WT Col-0 leaves, (iii) omg1 leaves and (iv) rbohd leaves were placed in DCF-DA solution immediately after wounding. In the WT plants green fluorescence around the wound site indicates the production of ROS. However, in the omg1 KO line and the rbohd KO line, ROS production was not detected. Images were taken on the Olympus BX51 compound scope at 10× magnification, exposure time 55 ms. Scale bars: 100 µm. (B) Quantification of fluorescence was performed using integrated density (IntDen) and corrected total cell fluorescence (CTCF) [CTCF = Integrated density − (Area of selected cell × Mean fluorescence of background readings)] in ImageJ. Statistical analyses were performed using Student’s t -test, bar graphs with different letters show significant difference ( P

    Journal: AoB Plants

    Article Title: A member of the CONSTANS-Like protein family is a putative regulator of reactive oxygen species homeostasis and spaceflight physiological adaptation

    doi: 10.1093/aobpla/ply075

    Figure Lengend Snippet: Reactive oxygen species (ROS) accumulation upon wounding and flg22 induction is altered in omg1 leaves compared to Col-0 leaves. (A and B) DCF-DA ROS assay performed on wounded leaf tissues reveals that OMG1 affects cellular ROS production. (A) DCF-DA is a fluorogenic dye that measures hydroxyl, peroxyl and other ROS activity within the cell. A pipette tip was used to wound the surface of the leaves from the WT line, omg1 KO line and rbohd KO line (a known ROS-deficient mutant). Upon wounding, ROS is usually produced around the site of injury. The DCF-DA reagent is then oxidized by ROS resulting in appearance of green fluorescence. Plants were wounded and immediately placed in the DCF-DA solution for 20 min before visualized on the light microscope. (i) WT Col-0 leaves without wounding, (ii) WT Col-0 leaves, (iii) omg1 leaves and (iv) rbohd leaves were placed in DCF-DA solution immediately after wounding. In the WT plants green fluorescence around the wound site indicates the production of ROS. However, in the omg1 KO line and the rbohd KO line, ROS production was not detected. Images were taken on the Olympus BX51 compound scope at 10× magnification, exposure time 55 ms. Scale bars: 100 µm. (B) Quantification of fluorescence was performed using integrated density (IntDen) and corrected total cell fluorescence (CTCF) [CTCF = Integrated density − (Area of selected cell × Mean fluorescence of background readings)] in ImageJ. Statistical analyses were performed using Student’s t -test, bar graphs with different letters show significant difference ( P

    Article Snippet: Leaves were then rinsed in standard buffer and observed under GFP bandpass filters using the Olympus BX51 compound light microscope at 10× magnification with 55 ms exposure time.

    Techniques: ROS Assay, Activity Assay, Transferring, Mutagenesis, Produced, Fluorescence, Light Microscopy, Mass Spectrometry

    Cellular localization of opsins in projection areas at different time points post-injection. ( a ) Retrogradely transduced neurons in layer III, as well as axons and dendrites in all cortical layers show high expression of opsin constructs in monkey O. A direct comparison of area LIP in animals O (8.5 weeks post-injection) and H (2 years post-injection) shows opsin expression in all parts of retrogradely transduced neurons, as well as potentials axonal projections from FEF in monkey O ( a , b ), but mainly in the cell body and dendrites in monkey H ( c ). Opsins were present in axonal projections to layer I of LIP in monkey O ( d ) but could be barely detected in layer I of LIP in monkey H ( e ). Retrogradely transduced neurons in layer III of area MT showed a similar distribution of opsins within cell bodies and dendrites in monkey O ( f ) and monkey H ( g ). Images were captured with a BX51 Olympus light microscope, controlled by Cell^B software, version 3.4 (Olympus).

    Journal: Scientific Reports

    Article Title: Histological assessment of optogenetic tools to study fronto-visual and fronto-parietal cortical networks in the rhesus macaque

    doi: 10.1038/s41598-020-67752-6

    Figure Lengend Snippet: Cellular localization of opsins in projection areas at different time points post-injection. ( a ) Retrogradely transduced neurons in layer III, as well as axons and dendrites in all cortical layers show high expression of opsin constructs in monkey O. A direct comparison of area LIP in animals O (8.5 weeks post-injection) and H (2 years post-injection) shows opsin expression in all parts of retrogradely transduced neurons, as well as potentials axonal projections from FEF in monkey O ( a , b ), but mainly in the cell body and dendrites in monkey H ( c ). Opsins were present in axonal projections to layer I of LIP in monkey O ( d ) but could be barely detected in layer I of LIP in monkey H ( e ). Retrogradely transduced neurons in layer III of area MT showed a similar distribution of opsins within cell bodies and dendrites in monkey O ( f ) and monkey H ( g ). Images were captured with a BX51 Olympus light microscope, controlled by Cell^B software, version 3.4 (Olympus).

    Article Snippet: Additional histological documentation (Fig. ) was made by a BX51 Olympus light microscope with a Color View I Camera, controlled by Cell^B software, version 3.4 (Olympus).

    Techniques: Injection, Expressing, Construct, Light Microscopy, Software