Structured Review

Olympus bx51 light microscope
Immunolocalization of xylan using the monoclonal antibody LM10. Labelling was carried out on 8 μm-thick transverse sections from stem tissues of eight-week-old plants. A1-I1: xylan immumolocalization in WT, irx9 and MlGT43 complemented irx9 lines. A2-T2: xylan immunolocalization in WT, irx14 and MlGT43 complemented irx14 lines. Signals were detected with Alexa Fluor488-conjugated secondary antibody and observed with a <t>BX51</t> fluorescence microscope (OLYMPUS). Bar = 50 μm
Bx51 Light Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bx51 light microscope/product/Olympus
Average 93 stars, based on 19 article reviews
Price from $9.99 to $1999.99
bx51 light microscope - by Bioz Stars, 2020-08
93/100 stars

Images

1) Product Images from "Functional conservation and divergence of Miscanthus lutarioriparius GT43 gene family in xylan biosynthesis"

Article Title: Functional conservation and divergence of Miscanthus lutarioriparius GT43 gene family in xylan biosynthesis

Journal: BMC Plant Biology

doi: 10.1186/s12870-016-0793-5

Immunolocalization of xylan using the monoclonal antibody LM10. Labelling was carried out on 8 μm-thick transverse sections from stem tissues of eight-week-old plants. A1-I1: xylan immumolocalization in WT, irx9 and MlGT43 complemented irx9 lines. A2-T2: xylan immunolocalization in WT, irx14 and MlGT43 complemented irx14 lines. Signals were detected with Alexa Fluor488-conjugated secondary antibody and observed with a BX51 fluorescence microscope (OLYMPUS). Bar = 50 μm
Figure Legend Snippet: Immunolocalization of xylan using the monoclonal antibody LM10. Labelling was carried out on 8 μm-thick transverse sections from stem tissues of eight-week-old plants. A1-I1: xylan immumolocalization in WT, irx9 and MlGT43 complemented irx9 lines. A2-T2: xylan immunolocalization in WT, irx14 and MlGT43 complemented irx14 lines. Signals were detected with Alexa Fluor488-conjugated secondary antibody and observed with a BX51 fluorescence microscope (OLYMPUS). Bar = 50 μm

Techniques Used: Fluorescence, Microscopy

2) Product Images from "Inflammaging increases susceptibility to cigarette smoke-induced COPD"

Article Title: Inflammaging increases susceptibility to cigarette smoke-induced COPD

Journal: Oncotarget

doi: 10.18632/oncotarget.4027

Aged mice develop emphysema and airway remodeling after 3 months of CS exposure A. Lung function measurements for dynamic lung compliance were performed in intubated animals after 2 months of CS exposure and in tracheostomized animals after 3 months of CS exposure. B. Representative micrographs of HE-stained lung tissue sections from young and old FA and CS-exposed mice; scale bar 200 μm. C. Quantitative measurement of emphysema was determined by design-based stereology of HE-stained lung tissue sections using an Olympus BX51 light microscope equipped with the computer-assisted stereological toolbox newCAST. D. Representative micrographs of Masson's Trichrome staining of lung tissue sections from young and old FA and CS-exposed mice; scale bar 100 μm. E. Total volume of airway collagen per basal membrane was determined via quantitative morphological assessment. Data were combined from 2 independent experiments with n = 8 and are given as mean values ± SD; one-way ANOVA following Bonferroni post test with ** p
Figure Legend Snippet: Aged mice develop emphysema and airway remodeling after 3 months of CS exposure A. Lung function measurements for dynamic lung compliance were performed in intubated animals after 2 months of CS exposure and in tracheostomized animals after 3 months of CS exposure. B. Representative micrographs of HE-stained lung tissue sections from young and old FA and CS-exposed mice; scale bar 200 μm. C. Quantitative measurement of emphysema was determined by design-based stereology of HE-stained lung tissue sections using an Olympus BX51 light microscope equipped with the computer-assisted stereological toolbox newCAST. D. Representative micrographs of Masson's Trichrome staining of lung tissue sections from young and old FA and CS-exposed mice; scale bar 100 μm. E. Total volume of airway collagen per basal membrane was determined via quantitative morphological assessment. Data were combined from 2 independent experiments with n = 8 and are given as mean values ± SD; one-way ANOVA following Bonferroni post test with ** p

Techniques Used: Mouse Assay, Staining, Light Microscopy

3) Product Images from "Functional conservation and divergence of Miscanthus lutarioriparius GT43 gene family in xylan biosynthesis"

Article Title: Functional conservation and divergence of Miscanthus lutarioriparius GT43 gene family in xylan biosynthesis

Journal: BMC Plant Biology

doi: 10.1186/s12870-016-0793-5

Immunolocalization of xylan using the monoclonal antibody LM10. Labelling was carried out on 8 μm-thick transverse sections from stem tissues of eight-week-old plants. A1-I1: xylan immumolocalization in WT, irx9 and MlGT43 complemented irx9 lines. A2-T2: xylan immunolocalization in WT, irx14 and MlGT43 complemented irx14 lines. Signals were detected with Alexa Fluor488-conjugated secondary antibody and observed with a BX51 fluorescence microscope (OLYMPUS). Bar = 50 μm
Figure Legend Snippet: Immunolocalization of xylan using the monoclonal antibody LM10. Labelling was carried out on 8 μm-thick transverse sections from stem tissues of eight-week-old plants. A1-I1: xylan immumolocalization in WT, irx9 and MlGT43 complemented irx9 lines. A2-T2: xylan immunolocalization in WT, irx14 and MlGT43 complemented irx14 lines. Signals were detected with Alexa Fluor488-conjugated secondary antibody and observed with a BX51 fluorescence microscope (OLYMPUS). Bar = 50 μm

Techniques Used: Fluorescence, Microscopy

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Microscopy:

Article Title: In Vitro Methods to Characterize the Effects of Tobacco and Nontobacco Products on Human Platelet Function
Article Snippet: .. Vacuum aspirator Incubator at 37°C 4°C storage Forceps, fine-pointed Microscope with differential interference contrast capabilities (DIC), and 40 and 100× powered lens. (Olympus BX51 light microscope (Olympus, Melville, NY)) Camera attached to microscope (SPOT Pursuit digital camera) Computer capable of running the appropriate imaging software (see manufacturer’s suggestions) Microscope Imaging Software (SPOT RT software (New Hyde Park, NY)) ..

Light Microscopy:

Article Title: Nanosensors for the Chemical Imaging of Acetylcholine Using Magnetic Resonance Imaging
Article Snippet: .. The sections were viewed with Olympus BX51 light microscope. .. Sections processed to determine nonspecific staining by following the same procedures, but with omission of the primary antibody, showed no immunohistochemical labeling.

Article Title: Functional conservation and divergence of Miscanthus lutarioriparius GT43 gene family in xylan biosynthesis
Article Snippet: .. The sections were photographed with a BX51 light microscope (OLYMPUS). .. For the immunolabelling, sections were incubated with the LM10 antibody (1/20 dilution) for 2 h, then washed three times with phosphate-buffered saline, followed by incubation with rabbit anti-rat Alexa Fluor488-conjugated secondary antibody (1/100 dilution) in the dark for 1 h. Images were captured using a BX51 light microscope (OLYMPUS) equipped with fluorescent light.

Article Title: NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly
Article Snippet: .. Ten fields were chosen randomly and imaged under an Olympus BX51 light microscope at ×20 magnification. ..

Article Title: Functional conservation and divergence of Miscanthus lutarioriparius GT43 gene family in xylan biosynthesis
Article Snippet: .. For the immunolabelling, sections were incubated with the LM10 antibody (1/20 dilution) for 2 h, then washed three times with phosphate-buffered saline, followed by incubation with rabbit anti-rat Alexa Fluor488-conjugated secondary antibody (1/100 dilution) in the dark for 1 h. Images were captured using a BX51 light microscope (OLYMPUS) equipped with fluorescent light. .. For transmission electron microscopy, samples were embedded in Spurr’s resin.

Article Title: Inflammaging increases susceptibility to cigarette smoke-induced COPD
Article Snippet: .. Quantitative morphometry Design-based stereology was used to analyze sections using an Olympus BX51 light microscope equipped with a computer-assisted stereological toolbox (newCAST, Visiopharm, Hoersholm, Denmark) on HE- or Masson's Trichrome stained lung tissue slides as previously described [ ]. ..

Article Title: Growth in Hyper-Concentrated Sweet Whey Triggers Multi Stress Tolerance and Spray Drying Survival in Lactobacillus casei BL23: From the Molecular Basis to New Perspectives for Sustainable Probiotic Production
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Article Title: Neuronal representation of environmental boundaries in egocentric coordinates
Article Snippet: .. The locations of lesions or the end of tetrode tracks were identified through the use of light microscopy and images (Supplementary Fig. ) were obtained using a Nikon DXM1200 camera mounted on an Olympus BX51 light microscope. ..

Incubation:

Article Title: Functional conservation and divergence of Miscanthus lutarioriparius GT43 gene family in xylan biosynthesis
Article Snippet: .. For the immunolabelling, sections were incubated with the LM10 antibody (1/20 dilution) for 2 h, then washed three times with phosphate-buffered saline, followed by incubation with rabbit anti-rat Alexa Fluor488-conjugated secondary antibody (1/100 dilution) in the dark for 1 h. Images were captured using a BX51 light microscope (OLYMPUS) equipped with fluorescent light. .. For transmission electron microscopy, samples were embedded in Spurr’s resin.

Imaging:

Article Title: In Vitro Methods to Characterize the Effects of Tobacco and Nontobacco Products on Human Platelet Function
Article Snippet: .. Vacuum aspirator Incubator at 37°C 4°C storage Forceps, fine-pointed Microscope with differential interference contrast capabilities (DIC), and 40 and 100× powered lens. (Olympus BX51 light microscope (Olympus, Melville, NY)) Camera attached to microscope (SPOT Pursuit digital camera) Computer capable of running the appropriate imaging software (see manufacturer’s suggestions) Microscope Imaging Software (SPOT RT software (New Hyde Park, NY)) ..

Staining:

Article Title: Inflammaging increases susceptibility to cigarette smoke-induced COPD
Article Snippet: .. Quantitative morphometry Design-based stereology was used to analyze sections using an Olympus BX51 light microscope equipped with a computer-assisted stereological toolbox (newCAST, Visiopharm, Hoersholm, Denmark) on HE- or Masson's Trichrome stained lung tissue slides as previously described [ ]. ..

Software:

Article Title: In Vitro Methods to Characterize the Effects of Tobacco and Nontobacco Products on Human Platelet Function
Article Snippet: .. Vacuum aspirator Incubator at 37°C 4°C storage Forceps, fine-pointed Microscope with differential interference contrast capabilities (DIC), and 40 and 100× powered lens. (Olympus BX51 light microscope (Olympus, Melville, NY)) Camera attached to microscope (SPOT Pursuit digital camera) Computer capable of running the appropriate imaging software (see manufacturer’s suggestions) Microscope Imaging Software (SPOT RT software (New Hyde Park, NY)) ..

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  • 89
    Olympus photobinocular light microscope olympus bx51
    The effect of HF diet on histopathological changes in the liver. HE-stained preparations were sectioned with blade thickness of 5 μ m and analyzed and photographed using a combined <t>photobinocular</t> light microscope Olympus <t>BX51.</t> (a) Control with normal liver histology /10x/; (b) rimonabant treated control chow diet fed group with normal liver histology /10x/; (c) liver histology in HF group shows mild steatosis with portal inflammatory infiltrate and focal necrotic changes in parenchyma /20x/; (d) liver histology in HFR group shows mild steatosis /10x/.
    Photobinocular Light Microscope Olympus Bx51, supplied by Olympus, used in various techniques. Bioz Stars score: 89/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/photobinocular light microscope olympus bx51/product/Olympus
    Average 89 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    photobinocular light microscope olympus bx51 - by Bioz Stars, 2020-08
    89/100 stars
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    92
    Olympus nanotubes optical microscopy
    ( a ) SEM image of P(PDI-DTT) <t>nanotubes</t> in a bunch. ( b ) TEM image of P(PDI-DTT) nanotubes dispersed on carbon-coated copper grids. ( c ) TEM image of single P(PDI-DTT) nanotube.
    Nanotubes Optical Microscopy, supplied by Olympus, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Olympus darkfield microscopy
    Intracellular study of the nanostars using <t>darkfield</t> microscopy and SERS. Notes: ( A ) Darkfield microscopy pictures where an increase of gold nanostars (yellow) inside the tumor cells (green) is visualized over time. ( B ) SERS spectrum of the corresponding cells labeled with nanostars, showing the highest intensity peak at 1,333 cm −1 (black arrow). Abbreviation: SERS, surface-enhanced Raman scattering.
    Darkfield Microscopy, supplied by Olympus, used in various techniques. Bioz Stars score: 89/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Olympus light brightfield microscope
    Sample preparation for LM and EM. ( a,b ) For LM, HVC (RA) neurons are retrogradely labeled by injecting a fluorescent dextran (fluoro-Ruby) into RA ( a ), and a single labeled neuron is targeted and filled with Neurobiotin under the guidance of 2-photon microscopy ( b ). ( c ) The tissue is then fixed and 100 µm parasagittal sections are sliced through the entirety of HVC. ( d,e ) Sections are further processed to stain Neurobiotin-labeled neurites ( d ) and then imaged in <t>brightfield</t> with a 100x objective ( e ). ( f ) For EM, HVC (RA) neurons are retrogradely labeled with biotinylated dextran (BDA). ( g,h ) A single 200 µm parasagittal section is taken from the center of HVC ( g ) and further processed to stain BDA-labeled neurites ( h ). ( i,j ) A cube of tissue from within the center of HVC is extracted and stained by ROTO (reduced osmium OTO) ( i ) before being imaged via SBEM ( j ). DOI: http://dx.doi.org/10.7554/eLife.24364.004
    Light Brightfield Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The effect of HF diet on histopathological changes in the liver. HE-stained preparations were sectioned with blade thickness of 5 μ m and analyzed and photographed using a combined photobinocular light microscope Olympus BX51. (a) Control with normal liver histology /10x/; (b) rimonabant treated control chow diet fed group with normal liver histology /10x/; (c) liver histology in HF group shows mild steatosis with portal inflammatory infiltrate and focal necrotic changes in parenchyma /20x/; (d) liver histology in HFR group shows mild steatosis /10x/.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Rimonabant Improves Oxidative/Nitrosative Stress in Mice with Nonalcoholic Fatty Liver Disease

    doi: 10.1155/2015/842108

    Figure Lengend Snippet: The effect of HF diet on histopathological changes in the liver. HE-stained preparations were sectioned with blade thickness of 5 μ m and analyzed and photographed using a combined photobinocular light microscope Olympus BX51. (a) Control with normal liver histology /10x/; (b) rimonabant treated control chow diet fed group with normal liver histology /10x/; (c) liver histology in HF group shows mild steatosis with portal inflammatory infiltrate and focal necrotic changes in parenchyma /20x/; (d) liver histology in HFR group shows mild steatosis /10x/.

    Article Snippet: Preparations were analyzed and photographed using a combined photobinocular light microscope Olympus BX51 equipped with Artcore 500MI artery, Co. Ltd. Japan Camera.

    Techniques: Staining, Light Microscopy

    ( a ) SEM image of P(PDI-DTT) nanotubes in a bunch. ( b ) TEM image of P(PDI-DTT) nanotubes dispersed on carbon-coated copper grids. ( c ) TEM image of single P(PDI-DTT) nanotube.

    Journal: Royal Society Open Science

    Article Title: Convenient fabrication of conjugated polymer semiconductor nanotubes and their application in organic electronics

    doi: 10.1098/rsos.180868

    Figure Lengend Snippet: ( a ) SEM image of P(PDI-DTT) nanotubes in a bunch. ( b ) TEM image of P(PDI-DTT) nanotubes dispersed on carbon-coated copper grids. ( c ) TEM image of single P(PDI-DTT) nanotube.

    Article Snippet: Characterization of nanotubes Optical microscopy (Olympus BX51) and scanning electron microscopy (SEM, Hitachi S-4800) were used to characterize the morphology of the nanotubes.

    Techniques: Transmission Electron Microscopy

    TEM images of P(PDI-DTT) nanotubes dispersed on carbon-coated copper grids. Preparation condition: ( a ) without container of chloroform in Petri dish; ( b ) with reverse side as the template.

    Journal: Royal Society Open Science

    Article Title: Convenient fabrication of conjugated polymer semiconductor nanotubes and their application in organic electronics

    doi: 10.1098/rsos.180868

    Figure Lengend Snippet: TEM images of P(PDI-DTT) nanotubes dispersed on carbon-coated copper grids. Preparation condition: ( a ) without container of chloroform in Petri dish; ( b ) with reverse side as the template.

    Article Snippet: Characterization of nanotubes Optical microscopy (Olympus BX51) and scanning electron microscopy (SEM, Hitachi S-4800) were used to characterize the morphology of the nanotubes.

    Techniques: Transmission Electron Microscopy

    Transfer curve of polymer nanotube.

    Journal: Royal Society Open Science

    Article Title: Convenient fabrication of conjugated polymer semiconductor nanotubes and their application in organic electronics

    doi: 10.1098/rsos.180868

    Figure Lengend Snippet: Transfer curve of polymer nanotube.

    Article Snippet: Characterization of nanotubes Optical microscopy (Olympus BX51) and scanning electron microscopy (SEM, Hitachi S-4800) were used to characterize the morphology of the nanotubes.

    Techniques:

    Intracellular study of the nanostars using darkfield microscopy and SERS. Notes: ( A ) Darkfield microscopy pictures where an increase of gold nanostars (yellow) inside the tumor cells (green) is visualized over time. ( B ) SERS spectrum of the corresponding cells labeled with nanostars, showing the highest intensity peak at 1,333 cm −1 (black arrow). Abbreviation: SERS, surface-enhanced Raman scattering.

    Journal: International Journal of Nanomedicine

    Article Title: Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

    doi: 10.2147/IJN.S91340

    Figure Lengend Snippet: Intracellular study of the nanostars using darkfield microscopy and SERS. Notes: ( A ) Darkfield microscopy pictures where an increase of gold nanostars (yellow) inside the tumor cells (green) is visualized over time. ( B ) SERS spectrum of the corresponding cells labeled with nanostars, showing the highest intensity peak at 1,333 cm −1 (black arrow). Abbreviation: SERS, surface-enhanced Raman scattering.

    Article Snippet: After fixation with 4% paraformaldehyde in PBS, these coverslips were mounted on a glass slide and visualized by darkfield microscopy (Olympus BX51 equipped with a U-DCW condenser; Olympus, Berchem, Belgium) or Raman microscopy (type alpha 300R, WITec; 785 nm, 40 mW, 5 seconds integration time, raster of 40×40 μm).

    Techniques: Microscopy, Labeling

    Ex vivo analysis of the nanostar accumulation in different tissues. Notes: ( A ) Darkfield images of the different tissues where nanostars (gold spots) were clearly visualized inside the liver, spleen and in lesser concentration at the tumor border. ( B ) Surface-enhanced Raman scattering spectra of the different tissues where a DTNB spectrum was observed for the spleen, liver, and tumor border. Abbreviation: DTNB, 5,5-dithio-bis-(2-nitrobenzoic acid).

    Journal: International Journal of Nanomedicine

    Article Title: Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

    doi: 10.2147/IJN.S91340

    Figure Lengend Snippet: Ex vivo analysis of the nanostar accumulation in different tissues. Notes: ( A ) Darkfield images of the different tissues where nanostars (gold spots) were clearly visualized inside the liver, spleen and in lesser concentration at the tumor border. ( B ) Surface-enhanced Raman scattering spectra of the different tissues where a DTNB spectrum was observed for the spleen, liver, and tumor border. Abbreviation: DTNB, 5,5-dithio-bis-(2-nitrobenzoic acid).

    Article Snippet: After fixation with 4% paraformaldehyde in PBS, these coverslips were mounted on a glass slide and visualized by darkfield microscopy (Olympus BX51 equipped with a U-DCW condenser; Olympus, Berchem, Belgium) or Raman microscopy (type alpha 300R, WITec; 785 nm, 40 mW, 5 seconds integration time, raster of 40×40 μm).

    Techniques: Ex Vivo, Concentration Assay

    Sample preparation for LM and EM. ( a,b ) For LM, HVC (RA) neurons are retrogradely labeled by injecting a fluorescent dextran (fluoro-Ruby) into RA ( a ), and a single labeled neuron is targeted and filled with Neurobiotin under the guidance of 2-photon microscopy ( b ). ( c ) The tissue is then fixed and 100 µm parasagittal sections are sliced through the entirety of HVC. ( d,e ) Sections are further processed to stain Neurobiotin-labeled neurites ( d ) and then imaged in brightfield with a 100x objective ( e ). ( f ) For EM, HVC (RA) neurons are retrogradely labeled with biotinylated dextran (BDA). ( g,h ) A single 200 µm parasagittal section is taken from the center of HVC ( g ) and further processed to stain BDA-labeled neurites ( h ). ( i,j ) A cube of tissue from within the center of HVC is extracted and stained by ROTO (reduced osmium OTO) ( i ) before being imaged via SBEM ( j ). DOI: http://dx.doi.org/10.7554/eLife.24364.004

    Journal: eLife

    Article Title: EM connectomics reveals axonal target variation in a sequence-generating network

    doi: 10.7554/eLife.24364

    Figure Lengend Snippet: Sample preparation for LM and EM. ( a,b ) For LM, HVC (RA) neurons are retrogradely labeled by injecting a fluorescent dextran (fluoro-Ruby) into RA ( a ), and a single labeled neuron is targeted and filled with Neurobiotin under the guidance of 2-photon microscopy ( b ). ( c ) The tissue is then fixed and 100 µm parasagittal sections are sliced through the entirety of HVC. ( d,e ) Sections are further processed to stain Neurobiotin-labeled neurites ( d ) and then imaged in brightfield with a 100x objective ( e ). ( f ) For EM, HVC (RA) neurons are retrogradely labeled with biotinylated dextran (BDA). ( g,h ) A single 200 µm parasagittal section is taken from the center of HVC ( g ) and further processed to stain BDA-labeled neurites ( h ). ( i,j ) A cube of tissue from within the center of HVC is extracted and stained by ROTO (reduced osmium OTO) ( i ) before being imaged via SBEM ( j ). DOI: http://dx.doi.org/10.7554/eLife.24364.004

    Article Snippet: In brief, a transmitted light brightfield microscope (Olympus BX51, Olympus, Japan), equipped with a motorized x-y-z stage (Maerzhaeuser, Germany), a narrow bandpass (546 ± 5 nm) illumination filter and a 100x magnification oil-immersion objective (numerical aperture 1.4) was used to acquire image stacks from consecutive 100 µm thick brain sections.

    Techniques: Sample Prep, Labeling, Microscopy, Staining