Structured Review

Olympus bx50 fluorescence microscope
MPT0B214 inhibits microtubule assembly in vivo . (A) KB and (B) KB-VIN10 cells were treated with test drugs or the same volume of DMSO in PBS as the control. After 24 h of incubation, cells were lysed in a lysis buffer. Cell lysates were centrifuged to separate polymerized microtubules (pellet) from tubulin dimmers (supernatant), as described in “ Material and Methods ”. After gel electrophoresis and transfer to the nitrocellulose membrane, α-tubulin was visualized by western blot analysis. (C) The effect of MPT0B214 on the organizations of the cellular microtubule network. Cells were treated with 500 nM colchicine, 500 nM paclitaxel, and MPT0B214 at concentrations of 50 nM, 250 nM, and 500 nM for 6 h. After incubation, cells were harvested and fixed with a fix solution. Fixed cells were reacted with monoclonal anti-α-tubulin antibody at room temperature for 2 h. After treatment with an FITC-conjugated secondary antibody, the cellular microtubules were observed using an <t>Olympus-BX50</t> fluorescence microscope.
Bx50 Fluorescence Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 91/100, based on 240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bx50 fluorescence microscope/product/Olympus
Average 91 stars, based on 240 article reviews
Price from $9.99 to $1999.99
bx50 fluorescence microscope - by Bioz Stars, 2020-08
91/100 stars

Images

1) Product Images from "A Novel Synthetic Microtubule Inhibitor, MPT0B214 Exhibits Antitumor Activity in Human Tumor Cells through Mitochondria-Dependent Intrinsic Pathway"

Article Title: A Novel Synthetic Microtubule Inhibitor, MPT0B214 Exhibits Antitumor Activity in Human Tumor Cells through Mitochondria-Dependent Intrinsic Pathway

Journal: PLoS ONE

doi: 10.1371/journal.pone.0058953

MPT0B214 inhibits microtubule assembly in vivo . (A) KB and (B) KB-VIN10 cells were treated with test drugs or the same volume of DMSO in PBS as the control. After 24 h of incubation, cells were lysed in a lysis buffer. Cell lysates were centrifuged to separate polymerized microtubules (pellet) from tubulin dimmers (supernatant), as described in “ Material and Methods ”. After gel electrophoresis and transfer to the nitrocellulose membrane, α-tubulin was visualized by western blot analysis. (C) The effect of MPT0B214 on the organizations of the cellular microtubule network. Cells were treated with 500 nM colchicine, 500 nM paclitaxel, and MPT0B214 at concentrations of 50 nM, 250 nM, and 500 nM for 6 h. After incubation, cells were harvested and fixed with a fix solution. Fixed cells were reacted with monoclonal anti-α-tubulin antibody at room temperature for 2 h. After treatment with an FITC-conjugated secondary antibody, the cellular microtubules were observed using an Olympus-BX50 fluorescence microscope.
Figure Legend Snippet: MPT0B214 inhibits microtubule assembly in vivo . (A) KB and (B) KB-VIN10 cells were treated with test drugs or the same volume of DMSO in PBS as the control. After 24 h of incubation, cells were lysed in a lysis buffer. Cell lysates were centrifuged to separate polymerized microtubules (pellet) from tubulin dimmers (supernatant), as described in “ Material and Methods ”. After gel electrophoresis and transfer to the nitrocellulose membrane, α-tubulin was visualized by western blot analysis. (C) The effect of MPT0B214 on the organizations of the cellular microtubule network. Cells were treated with 500 nM colchicine, 500 nM paclitaxel, and MPT0B214 at concentrations of 50 nM, 250 nM, and 500 nM for 6 h. After incubation, cells were harvested and fixed with a fix solution. Fixed cells were reacted with monoclonal anti-α-tubulin antibody at room temperature for 2 h. After treatment with an FITC-conjugated secondary antibody, the cellular microtubules were observed using an Olympus-BX50 fluorescence microscope.

Techniques Used: In Vivo, Incubation, Lysis, Nucleic Acid Electrophoresis, Western Blot, Fluorescence, Microscopy

2) Product Images from "Cinnamomum cassia Suppresses Caspase-9 through Stimulation of AKT1 in MCF-7 Cells but Not in MDA-MB-231 Cells"

Article Title: Cinnamomum cassia Suppresses Caspase-9 through Stimulation of AKT1 in MCF-7 Cells but Not in MDA-MB-231 Cells

Journal: PLoS ONE

doi: 10.1371/journal.pone.0145216

Morphological assessment of CE-treated and untreated MCF-7 and MDA-MB231 cells. A double fluorescent dye (AO/PI) staining method was used on cells after 24 h incubation with the IC 50 concentration of CE. Apoptosis characteristics such as, early apoptosis (ap) nuclear compaction (nc), chromatin condensation (cc), the membrane plasma blebbing (mb) and the mitotic cells (mt) were observed in CE-treated (C) MCF-7 and (D) MDA-MB231 cells using an Olympus BX50 fluorescence microscope. Untreated controls (A) MCF-7 and (B) MDA-MB-231 were included. (200 X) magnification morphology.
Figure Legend Snippet: Morphological assessment of CE-treated and untreated MCF-7 and MDA-MB231 cells. A double fluorescent dye (AO/PI) staining method was used on cells after 24 h incubation with the IC 50 concentration of CE. Apoptosis characteristics such as, early apoptosis (ap) nuclear compaction (nc), chromatin condensation (cc), the membrane plasma blebbing (mb) and the mitotic cells (mt) were observed in CE-treated (C) MCF-7 and (D) MDA-MB231 cells using an Olympus BX50 fluorescence microscope. Untreated controls (A) MCF-7 and (B) MDA-MB-231 were included. (200 X) magnification morphology.

Techniques Used: Multiple Displacement Amplification, Staining, Incubation, Concentration Assay, Fluorescence, Microscopy

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Staining:

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Article Snippet: .. Spirochetes were stained with the BacTrace FITC-conjugated goat anti- Bb antibody (Kirkegaard and Perry Laboratories) and observed by using an Olympus BX50 fluorescence microscope. ..

Fluorescence:

Article Title: Age‐dependent impact of CaV3.2 T‐type calcium channel deletion on myogenic tone and flow‐mediated vasodilatation in small arteries
Article Snippet: .. A BX50 fluorescence microscope (Olympus) equipped with a UPlanApo 40×/1.00 oil iris high‐quality quartz objective was used for inspection and images were acquired using a Retiga 6000 cooled monochrome CCD camera and Q‐Capture Pro7 software (Q‐Imaging Inc., Surrey, BC, Canada). .. All images were acquired using the exact same camera settings and the same type of final adjustments were applied using Photoshop CS6 (Adobe Systems Inc., San Jose, CA, USA).

Article Title: Cinnamomum cassia Suppresses Caspase-9 through Stimulation of AKT1 in MCF-7 Cells but Not in MDA-MB-231 Cells
Article Snippet: .. Slides were observed under Olympus BX50 fluorescence microscope within 30 min. .. Preparation of cell lysate MCF-7 and MDA-MB-231 cells were seeded into 12-well plates at 5× 106 cells/well and allowed to attach for 24 h. The cells were treated with 100 μg/ml of CE (IC70 ) and incubated at time points (6, 9, 12, 24 and 48 h).

Article Title: A manganese transporter, BB0219 (BmtA), is required for virulence by the Lyme disease spirochete, Borrelia burgdorferi
Article Snippet: .. Spirochetes were stained with the BacTrace FITC-conjugated goat anti- Bb antibody (Kirkegaard and Perry Laboratories) and observed by using an Olympus BX50 fluorescence microscope. ..

Article Title: Inhibition of cereal rust fungi by both class I and II defensins derived from the flowers of Nicotiana alata
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Article Title: Molecular cloning and transcriptional activity of a new Petunia calreticulin gene involved in pistil transmitting tract maturation, progamic phase, and double fertilization
Article Snippet: .. Images were acquired using an Olympus BX50 fluorescence microscope, Olympus XC50 digital color camera, and CellB software (Olympus Soft Imaging Solutions GmbH). .. In silico sequence analysis The Neighbor-joining tree estimated from distances between the plant CRT amino acid sequences was built by Mega version 4 http://www.megasoftware.net/ , using Tajima-Nei distance and 1000 bootstrap (Tamura et al. ).

Article Title: Matrix metalloproteinase-9 contributes to parenchymal hemorrhage and necrosis in the remnant liver after extended hepatectomy in mice
Article Snippet: .. The number of infiltrated neutrophils in the remnant liver was evaluated in five randomly captured 40 × high-power fields (hpf) with an Olympus BX50 fluorescence microscope (Olympus Optical, Tokyo, Japan). .. In situ gelatinolytic activity was determined using 20-μm cryostat sections for the EnzChek Gelatinase assay kit (Molecular Probes, Eugene, OR)[ ].

Article Title: A Novel Synthetic Microtubule Inhibitor, MPT0B214 Exhibits Antitumor Activity in Human Tumor Cells through Mitochondria-Dependent Intrinsic Pathway
Article Snippet: .. The images of cellular microtubules were captured with an Olympus BX50 fluorescence microscope (Dulles, VA). ..

Article Title: Identification and Characterization of Colletotrichum Species Associated with Bitter Rot Disease of Apple in South Korea
Article Snippet: .. Conidia of every isolate were characterized using conidia from 10 days old colonies on PDA media, mounted in a drop of lactophenol and examined using Olympus BX50 Fluorescence Microscope with digital camera (Olympus optical Co., Ltd, Japan), at least 24 conidia were measured for each isolate ( ). .. Appressoria were produced by a slide culture technique ( ).

Imaging:

Article Title: Molecular cloning and transcriptional activity of a new Petunia calreticulin gene involved in pistil transmitting tract maturation, progamic phase, and double fertilization
Article Snippet: .. Images were acquired using an Olympus BX50 fluorescence microscope, Olympus XC50 digital color camera, and CellB software (Olympus Soft Imaging Solutions GmbH). .. In silico sequence analysis The Neighbor-joining tree estimated from distances between the plant CRT amino acid sequences was built by Mega version 4 http://www.megasoftware.net/ , using Tajima-Nei distance and 1000 bootstrap (Tamura et al. ).

Microscopy:

Article Title: Age‐dependent impact of CaV3.2 T‐type calcium channel deletion on myogenic tone and flow‐mediated vasodilatation in small arteries
Article Snippet: .. A BX50 fluorescence microscope (Olympus) equipped with a UPlanApo 40×/1.00 oil iris high‐quality quartz objective was used for inspection and images were acquired using a Retiga 6000 cooled monochrome CCD camera and Q‐Capture Pro7 software (Q‐Imaging Inc., Surrey, BC, Canada). .. All images were acquired using the exact same camera settings and the same type of final adjustments were applied using Photoshop CS6 (Adobe Systems Inc., San Jose, CA, USA).

Article Title: Cinnamomum cassia Suppresses Caspase-9 through Stimulation of AKT1 in MCF-7 Cells but Not in MDA-MB-231 Cells
Article Snippet: .. Slides were observed under Olympus BX50 fluorescence microscope within 30 min. .. Preparation of cell lysate MCF-7 and MDA-MB-231 cells were seeded into 12-well plates at 5× 106 cells/well and allowed to attach for 24 h. The cells were treated with 100 μg/ml of CE (IC70 ) and incubated at time points (6, 9, 12, 24 and 48 h).

Article Title: A manganese transporter, BB0219 (BmtA), is required for virulence by the Lyme disease spirochete, Borrelia burgdorferi
Article Snippet: .. Spirochetes were stained with the BacTrace FITC-conjugated goat anti- Bb antibody (Kirkegaard and Perry Laboratories) and observed by using an Olympus BX50 fluorescence microscope. ..

Article Title: Inhibition of cereal rust fungi by both class I and II defensins derived from the flowers of Nicotiana alata
Article Snippet: .. Images were captured with an Olympus BX50 fluorescence microscope using an excitation wavelength of 460–490 nm and an MWIB filter. ..

Article Title: Molecular cloning and transcriptional activity of a new Petunia calreticulin gene involved in pistil transmitting tract maturation, progamic phase, and double fertilization
Article Snippet: .. Images were acquired using an Olympus BX50 fluorescence microscope, Olympus XC50 digital color camera, and CellB software (Olympus Soft Imaging Solutions GmbH). .. In silico sequence analysis The Neighbor-joining tree estimated from distances between the plant CRT amino acid sequences was built by Mega version 4 http://www.megasoftware.net/ , using Tajima-Nei distance and 1000 bootstrap (Tamura et al. ).

Article Title: Matrix metalloproteinase-9 contributes to parenchymal hemorrhage and necrosis in the remnant liver after extended hepatectomy in mice
Article Snippet: .. The number of infiltrated neutrophils in the remnant liver was evaluated in five randomly captured 40 × high-power fields (hpf) with an Olympus BX50 fluorescence microscope (Olympus Optical, Tokyo, Japan). .. In situ gelatinolytic activity was determined using 20-μm cryostat sections for the EnzChek Gelatinase assay kit (Molecular Probes, Eugene, OR)[ ].

Article Title: A Novel Synthetic Microtubule Inhibitor, MPT0B214 Exhibits Antitumor Activity in Human Tumor Cells through Mitochondria-Dependent Intrinsic Pathway
Article Snippet: .. The images of cellular microtubules were captured with an Olympus BX50 fluorescence microscope (Dulles, VA). ..

Article Title: Identification and Characterization of Colletotrichum Species Associated with Bitter Rot Disease of Apple in South Korea
Article Snippet: .. Conidia of every isolate were characterized using conidia from 10 days old colonies on PDA media, mounted in a drop of lactophenol and examined using Olympus BX50 Fluorescence Microscope with digital camera (Olympus optical Co., Ltd, Japan), at least 24 conidia were measured for each isolate ( ). .. Appressoria were produced by a slide culture technique ( ).

Software:

Article Title: Age‐dependent impact of CaV3.2 T‐type calcium channel deletion on myogenic tone and flow‐mediated vasodilatation in small arteries
Article Snippet: .. A BX50 fluorescence microscope (Olympus) equipped with a UPlanApo 40×/1.00 oil iris high‐quality quartz objective was used for inspection and images were acquired using a Retiga 6000 cooled monochrome CCD camera and Q‐Capture Pro7 software (Q‐Imaging Inc., Surrey, BC, Canada). .. All images were acquired using the exact same camera settings and the same type of final adjustments were applied using Photoshop CS6 (Adobe Systems Inc., San Jose, CA, USA).

Article Title: Molecular cloning and transcriptional activity of a new Petunia calreticulin gene involved in pistil transmitting tract maturation, progamic phase, and double fertilization
Article Snippet: .. Images were acquired using an Olympus BX50 fluorescence microscope, Olympus XC50 digital color camera, and CellB software (Olympus Soft Imaging Solutions GmbH). .. In silico sequence analysis The Neighbor-joining tree estimated from distances between the plant CRT amino acid sequences was built by Mega version 4 http://www.megasoftware.net/ , using Tajima-Nei distance and 1000 bootstrap (Tamura et al. ).

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    Olympus bx50 fluorescent microscope
    Effect of siRNA transfection on cellular morphology, and transfection efficiency of various siRNAs: (a) retinal endothelial cells untransfected, or transfected with H-Ras siRNA or scramble RNA were visualized under an Olympus <t>BX50</t> microscope and imaged
    Bx50 Fluorescent Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 89/100, based on 296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bx50 fluorescent microscope/product/Olympus
    Average 89 stars, based on 296 article reviews
    Price from $9.99 to $1999.99
    bx50 fluorescent microscope - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    Image Search Results


    Effect of siRNA transfection on cellular morphology, and transfection efficiency of various siRNAs: (a) retinal endothelial cells untransfected, or transfected with H-Ras siRNA or scramble RNA were visualized under an Olympus BX50 microscope and imaged

    Journal: Journal of cellular physiology

    Article Title: Diabetic Retinopathy and Signaling Mechanism for Activation of Matrix Metalloproteinase-9

    doi: 10.1002/jcp.22822

    Figure Lengend Snippet: Effect of siRNA transfection on cellular morphology, and transfection efficiency of various siRNAs: (a) retinal endothelial cells untransfected, or transfected with H-Ras siRNA or scramble RNA were visualized under an Olympus BX50 microscope and imaged

    Article Snippet: After treating the sections with 0.1% Tween-20, they were incubated with a mixture of two secondary antibodies (anti-rabbit-FITC conjugated and anti mouse-Texas red, Vector Laboratories, Burlington, CA) for 1 h. The slides were rinsed with PBS, mounted with DAPI containing mounting media (Vector Laboratories), and imaged with Olympus BX50 fluorescent microscope (20× magnification).

    Techniques: Transfection, Microscopy

    MPT0B214 inhibits microtubule assembly in vivo . (A) KB and (B) KB-VIN10 cells were treated with test drugs or the same volume of DMSO in PBS as the control. After 24 h of incubation, cells were lysed in a lysis buffer. Cell lysates were centrifuged to separate polymerized microtubules (pellet) from tubulin dimmers (supernatant), as described in “ Material and Methods ”. After gel electrophoresis and transfer to the nitrocellulose membrane, α-tubulin was visualized by western blot analysis. (C) The effect of MPT0B214 on the organizations of the cellular microtubule network. Cells were treated with 500 nM colchicine, 500 nM paclitaxel, and MPT0B214 at concentrations of 50 nM, 250 nM, and 500 nM for 6 h. After incubation, cells were harvested and fixed with a fix solution. Fixed cells were reacted with monoclonal anti-α-tubulin antibody at room temperature for 2 h. After treatment with an FITC-conjugated secondary antibody, the cellular microtubules were observed using an Olympus-BX50 fluorescence microscope.

    Journal: PLoS ONE

    Article Title: A Novel Synthetic Microtubule Inhibitor, MPT0B214 Exhibits Antitumor Activity in Human Tumor Cells through Mitochondria-Dependent Intrinsic Pathway

    doi: 10.1371/journal.pone.0058953

    Figure Lengend Snippet: MPT0B214 inhibits microtubule assembly in vivo . (A) KB and (B) KB-VIN10 cells were treated with test drugs or the same volume of DMSO in PBS as the control. After 24 h of incubation, cells were lysed in a lysis buffer. Cell lysates were centrifuged to separate polymerized microtubules (pellet) from tubulin dimmers (supernatant), as described in “ Material and Methods ”. After gel electrophoresis and transfer to the nitrocellulose membrane, α-tubulin was visualized by western blot analysis. (C) The effect of MPT0B214 on the organizations of the cellular microtubule network. Cells were treated with 500 nM colchicine, 500 nM paclitaxel, and MPT0B214 at concentrations of 50 nM, 250 nM, and 500 nM for 6 h. After incubation, cells were harvested and fixed with a fix solution. Fixed cells were reacted with monoclonal anti-α-tubulin antibody at room temperature for 2 h. After treatment with an FITC-conjugated secondary antibody, the cellular microtubules were observed using an Olympus-BX50 fluorescence microscope.

    Article Snippet: The images of cellular microtubules were captured with an Olympus BX50 fluorescence microscope (Dulles, VA).

    Techniques: In Vivo, Incubation, Lysis, Nucleic Acid Electrophoresis, Western Blot, Fluorescence, Microscopy

    Morphological assessment of CE-treated and untreated MCF-7 and MDA-MB231 cells. A double fluorescent dye (AO/PI) staining method was used on cells after 24 h incubation with the IC 50 concentration of CE. Apoptosis characteristics such as, early apoptosis (ap) nuclear compaction (nc), chromatin condensation (cc), the membrane plasma blebbing (mb) and the mitotic cells (mt) were observed in CE-treated (C) MCF-7 and (D) MDA-MB231 cells using an Olympus BX50 fluorescence microscope. Untreated controls (A) MCF-7 and (B) MDA-MB-231 were included. (200 X) magnification morphology.

    Journal: PLoS ONE

    Article Title: Cinnamomum cassia Suppresses Caspase-9 through Stimulation of AKT1 in MCF-7 Cells but Not in MDA-MB-231 Cells

    doi: 10.1371/journal.pone.0145216

    Figure Lengend Snippet: Morphological assessment of CE-treated and untreated MCF-7 and MDA-MB231 cells. A double fluorescent dye (AO/PI) staining method was used on cells after 24 h incubation with the IC 50 concentration of CE. Apoptosis characteristics such as, early apoptosis (ap) nuclear compaction (nc), chromatin condensation (cc), the membrane plasma blebbing (mb) and the mitotic cells (mt) were observed in CE-treated (C) MCF-7 and (D) MDA-MB231 cells using an Olympus BX50 fluorescence microscope. Untreated controls (A) MCF-7 and (B) MDA-MB-231 were included. (200 X) magnification morphology.

    Article Snippet: Slides were observed under Olympus BX50 fluorescence microscope within 30 min.

    Techniques: Multiple Displacement Amplification, Staining, Incubation, Concentration Assay, Fluorescence, Microscopy

    CD73 expression in pancreatic islets. (A) Frozen sections of pancreas from NOD and TLR9-/-NOD mice (n=3/group, 6-7 week-old, female) were stained with CD73 (green) and insulin (red) as described in Materials and Methods. The sections were examined and photographed using Olympus fluorescent microscope BX50 after staining. (B, C) CD73 expression in CD4 + and CD8 + T cells. Splenocytes, PLNs and isolated infiltrating immune cells from islets of 5-month-old male NOD mice were stained and examined by flow cytometry. (B) One representative FACS plot is shown. (C) Summary of the percentage of CD73 expressing cells in CD4 + and CD8 + T cells (n=5, *, p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: TLR9 deficiency promotes CD73 expression in T cells and diabetes protection in NOD mice

    doi: 10.4049/jimmunol.1300547

    Figure Lengend Snippet: CD73 expression in pancreatic islets. (A) Frozen sections of pancreas from NOD and TLR9-/-NOD mice (n=3/group, 6-7 week-old, female) were stained with CD73 (green) and insulin (red) as described in Materials and Methods. The sections were examined and photographed using Olympus fluorescent microscope BX50 after staining. (B, C) CD73 expression in CD4 + and CD8 + T cells. Splenocytes, PLNs and isolated infiltrating immune cells from islets of 5-month-old male NOD mice were stained and examined by flow cytometry. (B) One representative FACS plot is shown. (C) Summary of the percentage of CD73 expressing cells in CD4 + and CD8 + T cells (n=5, *, p

    Article Snippet: Pancreatic sections were examined and photographed using an Olympus fluorescent microscope BX50.

    Techniques: Expressing, Mouse Assay, Staining, Microscopy, Isolation, Flow Cytometry, Cytometry, FACS