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mouse microglial cell line bv2  (ATCC)


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    Structured Review

    ATCC mouse microglial cell line bv2
    Mouse Microglial Cell Line Bv2, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bv2+cells/EOC+2/pm41633359-382-0-5
    Average 95 stars, based on 104 article reviews
    mouse microglial cell line bv2 - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Oncogenic BRAF V600E induces microglial proliferation through extracellular signal-regulated kinase and neuronal death through c-Jun N-terminal kinase
    Article Snippet: Retroviral particles were produced by transducing HEK293T cells with the pBabe-Puro-BRAF-V600E plasmid (Addgene), the packaging plasmid gag/pol-Retroviral (Addgene), and the envelope plasmid MD2.G using Lipofectamine 2000. .. BV2 cells (ATCC, Manassas, VA, USA, Cat# CRL-2467, RRID: CVCL_5744) were cultured with high-glucose Dulbecco’s Modified Eagle Medium (DMEM; Thermo Fisher Scientific) containing 10% fetal bovine serum (FBS; Thermo Fisher Scientific) and high glucose DMEM/F12 (Thermo Fisher Scientific) containing 10% FBS at 37°C in 5% CO 2 under constant temperature and humidity. .. After transduction with BRAF V600E , BRAF WT , and vector lentivirus, respectively, for 24 hours, cells were cultured for 24 hours in DMEM/F12 without fetal bovine serum (FBS), and then medium and cells were collected for subsequent experiments.

    Article Title: A radiolabeled dendrimer non-invasively identifies and tracks innate immune cell activation in a mouse model of experimental autoimmune encephalomyelitis.
    Article Snippet: Samples were collected into K2EDTA tubes and shipped to Alturas Analytics (Moscow, ID) for bioanalytical measurement using a previously established high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) bioanalytical method. .. BV2 cells (ATCC, Manassas, VA, #CRL-2467) were cultured in the presence of 100ng/mL macrophage colony-stimulating factor (M-CSF, Thermo Fisher Scientific, Waltham, MA, #PMC2044) in serum-free Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, #11885084) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific, #15140122). .. Cells were treated with various concentrations ofH74DS3M8, and confluencewasobserved every 2 h in the Incucyte (Sartorius, Göttingen,Germany)maintained at 37 °C in 5%CO2.

    Article Title: A radiolabeled dendrimer non-invasively identifies and tracks innate immune cell activation in a mouse model of experimental autoimmune encephalomyelitis
    Article Snippet: Samples were collected into K2EDTA tubes and shipped to Alturas Analytics (Moscow, ID) for bioanalytical measurement using a previously established high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) bioanalytical method. .. BV2 cells (ATCC, Manassas, VA, #CRL-2467) were cultured in the presence of 100 ng/mL macrophage colony-stimulating factor (M-CSF, Thermo Fisher Scientific, Waltham, MA, #PMC2044) in serum-free Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, #11885084) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific, #15140122). .. Cells were treated with various concentrations of H74DS3M8, and confluence was observed every 2 h in the Incucyte (Sartorius, Göttingen, Germany) maintained at 37 °C in 5% CO 2 .

    Modification:

    Article Title: Oncogenic BRAF V600E induces microglial proliferation through extracellular signal-regulated kinase and neuronal death through c-Jun N-terminal kinase
    Article Snippet: Retroviral particles were produced by transducing HEK293T cells with the pBabe-Puro-BRAF-V600E plasmid (Addgene), the packaging plasmid gag/pol-Retroviral (Addgene), and the envelope plasmid MD2.G using Lipofectamine 2000. .. BV2 cells (ATCC, Manassas, VA, USA, Cat# CRL-2467, RRID: CVCL_5744) were cultured with high-glucose Dulbecco’s Modified Eagle Medium (DMEM; Thermo Fisher Scientific) containing 10% fetal bovine serum (FBS; Thermo Fisher Scientific) and high glucose DMEM/F12 (Thermo Fisher Scientific) containing 10% FBS at 37°C in 5% CO 2 under constant temperature and humidity. .. After transduction with BRAF V600E , BRAF WT , and vector lentivirus, respectively, for 24 hours, cells were cultured for 24 hours in DMEM/F12 without fetal bovine serum (FBS), and then medium and cells were collected for subsequent experiments.

    Article Title: A radiolabeled dendrimer non-invasively identifies and tracks innate immune cell activation in a mouse model of experimental autoimmune encephalomyelitis.
    Article Snippet: Samples were collected into K2EDTA tubes and shipped to Alturas Analytics (Moscow, ID) for bioanalytical measurement using a previously established high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) bioanalytical method. .. BV2 cells (ATCC, Manassas, VA, #CRL-2467) were cultured in the presence of 100ng/mL macrophage colony-stimulating factor (M-CSF, Thermo Fisher Scientific, Waltham, MA, #PMC2044) in serum-free Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, #11885084) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific, #15140122). .. Cells were treated with various concentrations ofH74DS3M8, and confluencewasobserved every 2 h in the Incucyte (Sartorius, Göttingen,Germany)maintained at 37 °C in 5%CO2.

    Article Title: A radiolabeled dendrimer non-invasively identifies and tracks innate immune cell activation in a mouse model of experimental autoimmune encephalomyelitis
    Article Snippet: Samples were collected into K2EDTA tubes and shipped to Alturas Analytics (Moscow, ID) for bioanalytical measurement using a previously established high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) bioanalytical method. .. BV2 cells (ATCC, Manassas, VA, #CRL-2467) were cultured in the presence of 100 ng/mL macrophage colony-stimulating factor (M-CSF, Thermo Fisher Scientific, Waltham, MA, #PMC2044) in serum-free Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, #11885084) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific, #15140122). .. Cells were treated with various concentrations of H74DS3M8, and confluence was observed every 2 h in the Incucyte (Sartorius, Göttingen, Germany) maintained at 37 °C in 5% CO 2 .



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