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GE Healthcare buffer solution pbs
Buffer Solution Pbs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/buffer solution pbs/product/GE Healthcare
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
buffer solution pbs - by Bioz Stars, 2020-09
99/100 stars

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GST Pulldown Assay:

Article Title: HLA-B-associated transcript 3 (Bat3) stabilizes and activates p53 in a HAUSP-dependent manner
Article Snippet: .. GST pulldown assay Purified His-Bat3 protein and Flag-HAUSP-truncated proteins were incubated with GST-tagged p53 protein or GST control protein in PBS buffer with glutathione agarose (GE Healthcare) for 2 h at 4°C. .. The incubated proteins were then washed and immunoblotted using anti-His, anti-Flag, or anti-GST antibodies.

Filtration:

Article Title: Expression and Characterization of Cryptococcus neoformans Recombinant App1
Article Snippet: .. One hundred microlitre of a 1 mg/ml solution of rApp1 dialyzed into PBS buffer was injected onto a Superdex 75 10/300 GL gel filtration column at 10°C (GE Healthcare Cat # 17-5174-01) using an AKTA fast-performance liquid chromatography system (GE Healthcare), pre-equilibrated with phosphate-buffered saline (PBS) supplemented with 137 mM NaCl, 2.7 mM KCl, 10 mM Na2 HPO4 , and 2 mM KH2 PO4 pH 7.6. .. The molecular weight markers comprised a mixture of aprotinin (6.5 kDa), ribonuclease A (13.7 kDa), carbonic anhydrase (29 kDa), ovalbumin (43 kDa), and conalbumin (75 kDa).

In Vitro:

Article Title: Magnetic Field Promotes Migration of Schwann Cells with Chondroitinase ABC (ChABC)-Loaded Superparamagnetic Nanoparticles Across Astrocyte Boundary in vitro
Article Snippet: .. Cryostat sections were incubated in PBS (HyClone), in conditioned medium collected from PEI-SPIONs/ChABC/SCs cultures, or in PBS (HyClone) containing ChABC (300 mU/mL) for 3 hrs at 37°C to confirm the bioactivity of ChABC to degrade CSPGs in the spinal cord tissue., Moreover, the ability of astrocytes to express CSPGs in vitro was also demonstrated. .. Immunostaining of the incubated sections or astrocytes for CS-56 and GFAP was performed as described in the immunofluorescence staining subsection below.

Size-exclusion Chromatography:

Article Title: Outer Membrane Vesicles Induce Immune Responses to Virulence Proteins and Protect against Colonization by Enterotoxigenic Escherichia coli ▿
Article Snippet: .. CexE-His6 was further purified and then exchanged into PBS buffer (pH 7.4) by size exclusion chromatography (Superdex 200 10/300 GL column; GE Healthcare). .. Heat-labile toxin was obtained from List Biological Laboratories, Campbell, CA.

Purification:

Article Title: Outer Membrane Vesicles Induce Immune Responses to Virulence Proteins and Protect against Colonization by Enterotoxigenic Escherichia coli ▿
Article Snippet: .. CexE-His6 was further purified and then exchanged into PBS buffer (pH 7.4) by size exclusion chromatography (Superdex 200 10/300 GL column; GE Healthcare). .. Heat-labile toxin was obtained from List Biological Laboratories, Campbell, CA.

Article Title: HLA-B-associated transcript 3 (Bat3) stabilizes and activates p53 in a HAUSP-dependent manner
Article Snippet: .. GST pulldown assay Purified His-Bat3 protein and Flag-HAUSP-truncated proteins were incubated with GST-tagged p53 protein or GST control protein in PBS buffer with glutathione agarose (GE Healthcare) for 2 h at 4°C. .. The incubated proteins were then washed and immunoblotted using anti-His, anti-Flag, or anti-GST antibodies.

Concentration Assay:

Article Title: Enhanced Expression and Functional Characterization of the Recombinant Putative Lysozyme-PMAP36 Fusion Protein
Article Snippet: .. The cell cultures at mid-logarithmic phase, OD600 of 0.2, were mixed with PBS (GE Healthcare) and PMAP36-P22 lysozyme fusion protein (final concentration: 64 μM) and incubated for 10 min at 37°C. .. We added EtBr (final concentration: 6 μM) to the reaction mixture and measured the fluorescence using fluorescence spectrometer (Infinite 200® Pro, TECAN, Austria); the excitation and emission wavelengths were 545 and 600 nm, respectively.

Incubation:

Article Title: HLA-B-associated transcript 3 (Bat3) stabilizes and activates p53 in a HAUSP-dependent manner
Article Snippet: .. GST pulldown assay Purified His-Bat3 protein and Flag-HAUSP-truncated proteins were incubated with GST-tagged p53 protein or GST control protein in PBS buffer with glutathione agarose (GE Healthcare) for 2 h at 4°C. .. The incubated proteins were then washed and immunoblotted using anti-His, anti-Flag, or anti-GST antibodies.

Article Title: Enhanced Expression and Functional Characterization of the Recombinant Putative Lysozyme-PMAP36 Fusion Protein
Article Snippet: .. The cell cultures at mid-logarithmic phase, OD600 of 0.2, were mixed with PBS (GE Healthcare) and PMAP36-P22 lysozyme fusion protein (final concentration: 64 μM) and incubated for 10 min at 37°C. .. We added EtBr (final concentration: 6 μM) to the reaction mixture and measured the fluorescence using fluorescence spectrometer (Infinite 200® Pro, TECAN, Austria); the excitation and emission wavelengths were 545 and 600 nm, respectively.

Article Title: Magnetic Field Promotes Migration of Schwann Cells with Chondroitinase ABC (ChABC)-Loaded Superparamagnetic Nanoparticles Across Astrocyte Boundary in vitro
Article Snippet: .. Cryostat sections were incubated in PBS (HyClone), in conditioned medium collected from PEI-SPIONs/ChABC/SCs cultures, or in PBS (HyClone) containing ChABC (300 mU/mL) for 3 hrs at 37°C to confirm the bioactivity of ChABC to degrade CSPGs in the spinal cord tissue., Moreover, the ability of astrocytes to express CSPGs in vitro was also demonstrated. .. Immunostaining of the incubated sections or astrocytes for CS-56 and GFAP was performed as described in the immunofluorescence staining subsection below.

Injection:

Article Title: Expression and Characterization of Cryptococcus neoformans Recombinant App1
Article Snippet: .. One hundred microlitre of a 1 mg/ml solution of rApp1 dialyzed into PBS buffer was injected onto a Superdex 75 10/300 GL gel filtration column at 10°C (GE Healthcare Cat # 17-5174-01) using an AKTA fast-performance liquid chromatography system (GE Healthcare), pre-equilibrated with phosphate-buffered saline (PBS) supplemented with 137 mM NaCl, 2.7 mM KCl, 10 mM Na2 HPO4 , and 2 mM KH2 PO4 pH 7.6. .. The molecular weight markers comprised a mixture of aprotinin (6.5 kDa), ribonuclease A (13.7 kDa), carbonic anhydrase (29 kDa), ovalbumin (43 kDa), and conalbumin (75 kDa).

Binding Assay:

Article Title: TERT alleviates irradiation-induced late rectal injury by reducing hypoxia-induced ROS levels through the activation of NF-κB and autophagy
Article Snippet: .. Detection of apoptosis The digested cells were washed twice with PBS (pH 7.4; HyClone), centrifuged for 5 min at 37°C, and resuspended in binding buffer. .. They were then stained using the Annexin V-FITC Apoptosis Detection kit, followed by the addition of propidium iodide (PI) (both from BD Biosciences, San Jose, CA, USA).

Liquid Chromatography:

Article Title: Expression and Characterization of Cryptococcus neoformans Recombinant App1
Article Snippet: .. One hundred microlitre of a 1 mg/ml solution of rApp1 dialyzed into PBS buffer was injected onto a Superdex 75 10/300 GL gel filtration column at 10°C (GE Healthcare Cat # 17-5174-01) using an AKTA fast-performance liquid chromatography system (GE Healthcare), pre-equilibrated with phosphate-buffered saline (PBS) supplemented with 137 mM NaCl, 2.7 mM KCl, 10 mM Na2 HPO4 , and 2 mM KH2 PO4 pH 7.6. .. The molecular weight markers comprised a mixture of aprotinin (6.5 kDa), ribonuclease A (13.7 kDa), carbonic anhydrase (29 kDa), ovalbumin (43 kDa), and conalbumin (75 kDa).

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  • 90
    GE Healthcare strep tactin hrp detection solution
    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a <t>Strep-Tactin®-HRP</t> conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).
    Strep Tactin Hrp Detection Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/strep tactin hrp detection solution/product/GE Healthcare
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    96
    GE Healthcare pbs
    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a <t>Strep-Tactin®-HRP</t> conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).
    Pbs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 343 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs/product/GE Healthcare
    Average 96 stars, based on 343 article reviews
    Price from $9.99 to $1999.99
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    99
    GE Healthcare bovine serum albumin solution
    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a <t>Strep-Tactin®-HRP</t> conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).
    Bovine Serum Albumin Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine serum albumin solution/product/GE Healthcare
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bovine serum albumin solution - by Bioz Stars, 2020-09
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    92
    GE Healthcare pbs solution
    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a <t>Strep-Tactin®-HRP</t> conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).
    Pbs Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs solution/product/GE Healthcare
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
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    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a Strep-Tactin®-HRP conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).

    Journal: PLoS ONE

    Article Title: Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems

    doi: 10.1371/journal.pone.0152386

    Figure Lengend Snippet: Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a Strep-Tactin®-HRP conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).

    Article Snippet: The membrane was blocked with 1 x PBS, 0.5% Tween 20 supplemented with 3% BSA for 1 h. Then the block solution was discarded and Strep-Tactin®-HRP detection solution (1:100000 dilution in 1 x PBS, 0.1% Tween 20) was directly added to the membrane for 1 h. The membrane was then washed twice with 1 x PBS, 0.1% Tween-20 and twice with 1 x PBS for 1 min before the membrane was developed using ECL™ (GE Healthcare Europe GmbH, Freiburg, Germany, Cat. no.: RPN2232).

    Techniques: Expressing, Transfection, Sequencing, Fluorescence, Microscopy, Western Blot