buffer rlt plus  (Qiagen)


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    Structured Review

    Qiagen buffer rlt plus
    Buffer Rlt Plus, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/buffer rlt plus/product/Qiagen
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    buffer rlt plus - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Association between single nucleotide polymorphisms in TLR4, TLR2, TLR9, VDR, NOS2 and CCL5 genes with acute viral bronchiolitis.
    Article Snippet: The cell pellet was then resuspended in buffer RLT Plus and DNA and RNA isolation was performed using the AllPrep DNA/RNA Mini kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol. .. PCR was conducted in a thermocycler Mastercycler Eppendorf® with vapo.protect™ (São Paulo, Brazil) based on the manufacturer's protocol in a final volume of 20 μL containing 3 μL of cDNA (or 1.5 μL cDNA and 1.5 μL DNA; concentration included was 100 ng/μL), 4 μL of 5× RV15 ACE primer (A, B, or C), 3 μL of 8-MOP (8-Methoxypsoralen) solution, and 10 μL of 2× master mix (DNA polymerase and buffer containing dNTPs and dye).

    Isolation:

    Article Title: Association between single nucleotide polymorphisms in TLR4, TLR2, TLR9, VDR, NOS2 and CCL5 genes with acute viral bronchiolitis.
    Article Snippet: .. The cell pellet was then resuspended in buffer RLT Plus and DNA and RNA isolation was performed using the AllPrep DNA/RNA Mini kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol. .. Virus screening cDNA was synthesized using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems/Thermo Fisher Scientific, São Paulo, Brazil) according to the manufacturer's protocol.

    Synthesized:

    Article Title: Association between single nucleotide polymorphisms in TLR4, TLR2, TLR9, VDR, NOS2 and CCL5 genes with acute viral bronchiolitis.
    Article Snippet: The cell pellet was then resuspended in buffer RLT Plus and DNA and RNA isolation was performed using the AllPrep DNA/RNA Mini kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol. .. Virus screening cDNA was synthesized using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems/Thermo Fisher Scientific, São Paulo, Brazil) according to the manufacturer's protocol.

    Concentration Assay:

    Article Title: Association between single nucleotide polymorphisms in TLR4, TLR2, TLR9, VDR, NOS2 and CCL5 genes with acute viral bronchiolitis.
    Article Snippet: The cell pellet was then resuspended in buffer RLT Plus and DNA and RNA isolation was performed using the AllPrep DNA/RNA Mini kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol. .. PCR was conducted in a thermocycler Mastercycler Eppendorf® with vapo.protect™ (São Paulo, Brazil) based on the manufacturer's protocol in a final volume of 20 μL containing 3 μL of cDNA (or 1.5 μL cDNA and 1.5 μL DNA; concentration included was 100 ng/μL), 4 μL of 5× RV15 ACE primer (A, B, or C), 3 μL of 8-MOP (8-Methoxypsoralen) solution, and 10 μL of 2× master mix (DNA polymerase and buffer containing dNTPs and dye).

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    Qiagen rneasy micro kit
    Optimization of EBC ‐based expression analysis for LC diagnosis RTube is suitable for RNA isolation. Two main EBC collection devices, RTube and TurboDECCS, were compared for the total RNA yield ( y ‐axis, ng) obtained using the <t>QIAGEN</t> <t>RNeasy</t> Micro kit and 500 μl EBC as starting material. Triangles and rhombuses are used to denote RNA yield of each individual sample using EBCs collected from the different EBC collection devices. Data are represented as mean ± s.e.m.; n = 6. P ‐values after one‐way ANOVA. 500 μl of EBC is optimal for RNA isolation. Total RNA isolation with the RNeasy Micro kit was performed using 200, 350, 500, or 1,000 μl EBC as starting material. Data are represented as mean ± s.e.m.; n = 4. The High Capacity cDNA Reverse Transcriptase kit is more efficient than EpiScript Reverse Transcriptase. Two RT kits were tested for qRT–PCR‐based analysis of GATA6 Ad. CT values were plotted. Each dot represents the CT value for a technical triplicate. Data are represented as mean ± s.e.m.; n = 3. Serial dilution of cDNA template to determine the linear range of detection of GATA6 Ad. cDNA from control EBC was serially diluted and used as template for qRT–PCR‐based expression analysis of GATA6 Ad. Each dot represents the CT value for a technical triplicate. Data are represented as mean ± s.e.m.; n = 3. Delayed snap‐freezing of EBC after collection compromises mRNA integrity. Top, schematic representation of the precursor mRNAs from GAPDH (E) and HPRT1 (F) showing exons (boxes), introns (lines), and location of primer pairs (arrowheads) used for qRT–PCR‐based expression analysis. Bottom, EBCs were collected and incubated on ice for 0, 5, and 15 min prior to snap‐freezing in liquid nitrogen. Expression of GAPDH and HPRT1 was determined in the EBCs using the indicated primers, and the expression ratios (5′/3′) of each gene were calculated as indicators of mRNA integrity. RNA purified from EBCs with expression ratios of GAPDH and HPRT1 between 0.75 and 1.5 (dashed lines) was considered as acceptable for further analysis. Data are represented as mean ± s.e.m.; n = 3. Each colored triangle represents one individual. EBCs should be thawed on ice, for a maximum of 15 min, before further processing. EBCs, that were stored at −80°C, were thawed on ice (green line) or 25°C (red dashed line) for 15, 30, and 60 min. GAPDH 5′/3′ expression ratios were determined as in (E) as indicators of mRNA integrity. Each triangle refers to the 5′/3′ expression ratio for GAPDH of one sample. Each sample was measured in triplicates and the mean was used for the calculation of the ratio. Long‐term storage at −80°C or transportation on dry ice did not compromise mRNA integrity. EBCs were collected either in Germany (GER, green line) or in Mexico (MEX, red dashed line) and subsequently transported to Germany on dry ice. EBCs were stored at −80°C for 7, 30, or 365 days before they were thawed on ice and further processed in less than 15 min. GAPDH 5′/3′ expression ratios were determined as in (E) as indicators of mRNA integrity.
    Rneasy Micro Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2425 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy micro kit/product/Qiagen
    Average 99 stars, based on 2425 article reviews
    Price from $9.99 to $1999.99
    rneasy micro kit - by Bioz Stars, 2020-04
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    hsc  (Qiagen)
    92
    Qiagen hsc
    G-CSF-induced <t>HSC</t> mobilization requires signals from the muscarinic receptor type-1 ( Chrm1 ) (A) HSCs/mL following G-CSF mobilization in wild-type mice treated with saline or Scopolamine hydrobromide (Scop 1mg/kg; n = 5–8). HSCs defined as Lineage − <t>Sca1</t> + cKit + Flt3 − . (B) Schematic of G-CSF-induced mobilization and analyses. Wild-type mice are denoted by black bars and Chrm1 −/− mice by white bars. (C) HSCs/ml of peripheral blood (Lineage − Sca1 + cKit + Flt3 − ; n = 12–16) ( D) Colony-forming units/ml of peripheral blood determined in vitro (CFU-C; n = 6–13). (E) Lineage − Sca1 + cKit + (LSK) cells/femur at steady state (n = 9). ( F) Reconstitution after competitive BM transplantation of mobilized blood into irradiated hosts ( G) Tri-lineage engraftment of donor cells 16 weeks post transplantation: B220 + cells (B) CD4/CD8 + cells (T) and Mac1 + cells (M; n= 6–7). * p
    Hsc, supplied by Qiagen, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hsc/product/Qiagen
    Average 92 stars, based on 1 article reviews
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    99
    Qiagen buffer rlt plus
    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer <t>RLT</t> <t>Plus</t> ™ prior to RNA extraction.
    Buffer Rlt Plus, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/buffer rlt plus/product/Qiagen
    Average 99 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    buffer rlt plus - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Optimization of EBC ‐based expression analysis for LC diagnosis RTube is suitable for RNA isolation. Two main EBC collection devices, RTube and TurboDECCS, were compared for the total RNA yield ( y ‐axis, ng) obtained using the QIAGEN RNeasy Micro kit and 500 μl EBC as starting material. Triangles and rhombuses are used to denote RNA yield of each individual sample using EBCs collected from the different EBC collection devices. Data are represented as mean ± s.e.m.; n = 6. P ‐values after one‐way ANOVA. 500 μl of EBC is optimal for RNA isolation. Total RNA isolation with the RNeasy Micro kit was performed using 200, 350, 500, or 1,000 μl EBC as starting material. Data are represented as mean ± s.e.m.; n = 4. The High Capacity cDNA Reverse Transcriptase kit is more efficient than EpiScript Reverse Transcriptase. Two RT kits were tested for qRT–PCR‐based analysis of GATA6 Ad. CT values were plotted. Each dot represents the CT value for a technical triplicate. Data are represented as mean ± s.e.m.; n = 3. Serial dilution of cDNA template to determine the linear range of detection of GATA6 Ad. cDNA from control EBC was serially diluted and used as template for qRT–PCR‐based expression analysis of GATA6 Ad. Each dot represents the CT value for a technical triplicate. Data are represented as mean ± s.e.m.; n = 3. Delayed snap‐freezing of EBC after collection compromises mRNA integrity. Top, schematic representation of the precursor mRNAs from GAPDH (E) and HPRT1 (F) showing exons (boxes), introns (lines), and location of primer pairs (arrowheads) used for qRT–PCR‐based expression analysis. Bottom, EBCs were collected and incubated on ice for 0, 5, and 15 min prior to snap‐freezing in liquid nitrogen. Expression of GAPDH and HPRT1 was determined in the EBCs using the indicated primers, and the expression ratios (5′/3′) of each gene were calculated as indicators of mRNA integrity. RNA purified from EBCs with expression ratios of GAPDH and HPRT1 between 0.75 and 1.5 (dashed lines) was considered as acceptable for further analysis. Data are represented as mean ± s.e.m.; n = 3. Each colored triangle represents one individual. EBCs should be thawed on ice, for a maximum of 15 min, before further processing. EBCs, that were stored at −80°C, were thawed on ice (green line) or 25°C (red dashed line) for 15, 30, and 60 min. GAPDH 5′/3′ expression ratios were determined as in (E) as indicators of mRNA integrity. Each triangle refers to the 5′/3′ expression ratio for GAPDH of one sample. Each sample was measured in triplicates and the mean was used for the calculation of the ratio. Long‐term storage at −80°C or transportation on dry ice did not compromise mRNA integrity. EBCs were collected either in Germany (GER, green line) or in Mexico (MEX, red dashed line) and subsequently transported to Germany on dry ice. EBCs were stored at −80°C for 7, 30, or 365 days before they were thawed on ice and further processed in less than 15 min. GAPDH 5′/3′ expression ratios were determined as in (E) as indicators of mRNA integrity.

    Journal: EMBO Molecular Medicine

    Article Title: Non‐invasive lung cancer diagnosis by detection of GATA6 and NKX2‐1 isoforms in exhaled breath condensate

    doi: 10.15252/emmm.201606382

    Figure Lengend Snippet: Optimization of EBC ‐based expression analysis for LC diagnosis RTube is suitable for RNA isolation. Two main EBC collection devices, RTube and TurboDECCS, were compared for the total RNA yield ( y ‐axis, ng) obtained using the QIAGEN RNeasy Micro kit and 500 μl EBC as starting material. Triangles and rhombuses are used to denote RNA yield of each individual sample using EBCs collected from the different EBC collection devices. Data are represented as mean ± s.e.m.; n = 6. P ‐values after one‐way ANOVA. 500 μl of EBC is optimal for RNA isolation. Total RNA isolation with the RNeasy Micro kit was performed using 200, 350, 500, or 1,000 μl EBC as starting material. Data are represented as mean ± s.e.m.; n = 4. The High Capacity cDNA Reverse Transcriptase kit is more efficient than EpiScript Reverse Transcriptase. Two RT kits were tested for qRT–PCR‐based analysis of GATA6 Ad. CT values were plotted. Each dot represents the CT value for a technical triplicate. Data are represented as mean ± s.e.m.; n = 3. Serial dilution of cDNA template to determine the linear range of detection of GATA6 Ad. cDNA from control EBC was serially diluted and used as template for qRT–PCR‐based expression analysis of GATA6 Ad. Each dot represents the CT value for a technical triplicate. Data are represented as mean ± s.e.m.; n = 3. Delayed snap‐freezing of EBC after collection compromises mRNA integrity. Top, schematic representation of the precursor mRNAs from GAPDH (E) and HPRT1 (F) showing exons (boxes), introns (lines), and location of primer pairs (arrowheads) used for qRT–PCR‐based expression analysis. Bottom, EBCs were collected and incubated on ice for 0, 5, and 15 min prior to snap‐freezing in liquid nitrogen. Expression of GAPDH and HPRT1 was determined in the EBCs using the indicated primers, and the expression ratios (5′/3′) of each gene were calculated as indicators of mRNA integrity. RNA purified from EBCs with expression ratios of GAPDH and HPRT1 between 0.75 and 1.5 (dashed lines) was considered as acceptable for further analysis. Data are represented as mean ± s.e.m.; n = 3. Each colored triangle represents one individual. EBCs should be thawed on ice, for a maximum of 15 min, before further processing. EBCs, that were stored at −80°C, were thawed on ice (green line) or 25°C (red dashed line) for 15, 30, and 60 min. GAPDH 5′/3′ expression ratios were determined as in (E) as indicators of mRNA integrity. Each triangle refers to the 5′/3′ expression ratio for GAPDH of one sample. Each sample was measured in triplicates and the mean was used for the calculation of the ratio. Long‐term storage at −80°C or transportation on dry ice did not compromise mRNA integrity. EBCs were collected either in Germany (GER, green line) or in Mexico (MEX, red dashed line) and subsequently transported to Germany on dry ice. EBCs were stored at −80°C for 7, 30, or 365 days before they were thawed on ice and further processed in less than 15 min. GAPDH 5′/3′ expression ratios were determined as in (E) as indicators of mRNA integrity.

    Article Snippet: Exosome pellets were lysed in 350 μl RLT Plus Buffer (RNeasy Micro Kit, Qiagen), and 200 ng of 16S‐ and 23S‐ribosomal Spike‐In RNA (Roche) was added to the lysate.

    Techniques: Expressing, Isolation, Quantitative RT-PCR, Serial Dilution, Incubation, Purification

    G-CSF-induced HSC mobilization requires signals from the muscarinic receptor type-1 ( Chrm1 ) (A) HSCs/mL following G-CSF mobilization in wild-type mice treated with saline or Scopolamine hydrobromide (Scop 1mg/kg; n = 5–8). HSCs defined as Lineage − Sca1 + cKit + Flt3 − . (B) Schematic of G-CSF-induced mobilization and analyses. Wild-type mice are denoted by black bars and Chrm1 −/− mice by white bars. (C) HSCs/ml of peripheral blood (Lineage − Sca1 + cKit + Flt3 − ; n = 12–16) ( D) Colony-forming units/ml of peripheral blood determined in vitro (CFU-C; n = 6–13). (E) Lineage − Sca1 + cKit + (LSK) cells/femur at steady state (n = 9). ( F) Reconstitution after competitive BM transplantation of mobilized blood into irradiated hosts ( G) Tri-lineage engraftment of donor cells 16 weeks post transplantation: B220 + cells (B) CD4/CD8 + cells (T) and Mac1 + cells (M; n= 6–7). * p

    Journal: Cell stem cell

    Article Title: Cholinergic signals from the CNS regulate G-CSF-mediated HSC mobilization from bone marrow via a glucocorticoid signaling relay

    doi: 10.1016/j.stem.2017.01.002

    Figure Lengend Snippet: G-CSF-induced HSC mobilization requires signals from the muscarinic receptor type-1 ( Chrm1 ) (A) HSCs/mL following G-CSF mobilization in wild-type mice treated with saline or Scopolamine hydrobromide (Scop 1mg/kg; n = 5–8). HSCs defined as Lineage − Sca1 + cKit + Flt3 − . (B) Schematic of G-CSF-induced mobilization and analyses. Wild-type mice are denoted by black bars and Chrm1 −/− mice by white bars. (C) HSCs/ml of peripheral blood (Lineage − Sca1 + cKit + Flt3 − ; n = 12–16) ( D) Colony-forming units/ml of peripheral blood determined in vitro (CFU-C; n = 6–13). (E) Lineage − Sca1 + cKit + (LSK) cells/femur at steady state (n = 9). ( F) Reconstitution after competitive BM transplantation of mobilized blood into irradiated hosts ( G) Tri-lineage engraftment of donor cells 16 weeks post transplantation: B220 + cells (B) CD4/CD8 + cells (T) and Mac1 + cells (M; n= 6–7). * p

    Article Snippet: RNA for microarray analysis was isolated by sorting HSC (Lineage− Sca1+ cKit+ Flt3− directly into RLT buffer from the RNeasy Plus Micro kit (Qiagen) according to the manufacturercs instructions.

    Techniques: Mouse Assay, In Vitro, Transplantation Assay, Irradiation

    Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer RLT Plus ™ prior to RNA extraction.

    Journal: Brain, behavior, and immunity

    Article Title: Developmental alcohol exposure impairs synaptic plasticity without overtly altering microglial function in mouse visual cortex

    doi: 10.1016/j.bbi.2017.09.003

    Figure Lengend Snippet: Flow cytometry for isolation of microglia from early adolescent mouse visual cortex. (A) Schematic illustrating the use of fluorescence-activated cell sorting (FACS) to isolate microglia from P28 bilateral V1b. Gating scheme that was used for all samples shows (B) selection of the population containing cells, including microglia; (C) selection of singlets; (D) selection of the propidium iodide (PI) negative population representing live cells; and (E) selection of CD11b+ CD45low microglia that were collected directly into Buffer RLT Plus ™ prior to RNA extraction.

    Article Snippet: For immediate cell lysis and preservation of RNA, microglia (CD11b+, CD45 low) were sorted directly into 1.5mL microcentrifuge tubes (Axygen 311-08-051) containing 600μL Buffer RLT PLUS (Qiagen, 1053393) and 6μL β-mercaptoethanol at 4°C, prepared just prior to sorting.

    Techniques: Flow Cytometry, Cytometry, Isolation, Fluorescence, FACS, Selection, RNA Extraction

    Magnaporthe oryzae inactivation methods before 3 H-FLC treatment. 3 H-FLC accumulation was measured after 24 h in samples that were exposed to various inactivation methods. (Live): no inactivation, (HK): 95°C for 30 min, (AMB): 8 μg/ml of amphotericin B, (Lysis): 600 μL Qiagen RLT buffer, (CFG): 16 μg/ml of caspofungin. Error bars represent standard deviation for each condition. Asterisk indicates a statistical significance of P

    Journal: Frontiers in Microbiology

    Article Title: Accumulation of Azole Drugs in the Fungal Plant Pathogen Magnaporthe oryzae Is the Result of Facilitated Diffusion Influx

    doi: 10.3389/fmicb.2017.01320

    Figure Lengend Snippet: Magnaporthe oryzae inactivation methods before 3 H-FLC treatment. 3 H-FLC accumulation was measured after 24 h in samples that were exposed to various inactivation methods. (Live): no inactivation, (HK): 95°C for 30 min, (AMB): 8 μg/ml of amphotericin B, (Lysis): 600 μL Qiagen RLT buffer, (CFG): 16 μg/ml of caspofungin. Error bars represent standard deviation for each condition. Asterisk indicates a statistical significance of P

    Article Snippet: Methods of cell inactivation included treatment with high concentrations of AMB (8 μg/ml), Qiagen RLT Lysis solution (600 μL), and CFG (16 μg/ml).

    Techniques: Lysis, Standard Deviation